Our sexually reproducing S. mediterranea (Smed) stocks are descendants of animals collected in Sardinia by Dr. Maria Pala in 1999. We maintain Smed in 1x Montjuic water at 20˚C in the dark, and feed them homogenized beef liver as described in (Cebrià and Newmark, 2005). Our worms are currently housed in static and recirculating cultures. Static cultures subjected to intensive cutting and/or feeding regimens are supplemented with 100 µg/mL gentamicin sulfate (Gemini Bioproducts, #400-100P). For further information about recirculating systems for planaria culture, please contact SIMR Aquatics Facility Supervisor Shane Merryman (firstname.lastname@example.org).
Egg capsules are collected daily from outbred cohorts of sexually mature adults cultured at low density (~6-8 animals per 400 mL culture), and are stored in dated petri dishes at 20˚C in constant darkness until use. At collection, egg capsules are deemed to be 1 day post egg capsule deposition. To promote fertility, sexually mature animals used for egg capsule collections are replaced every 3-4 months with either juveniles (6-8 weeks post hatching) or adult regenerates (6-8 weeks post cut).