Timeline: days (d) post egg capsule deposition at 20˚C. Gray bars indicate time windows for RNA-Sequencing samples. Double headed arrows: time windows for Stages (S) S1-S8.
Click here for Single Embryo RNA-Sequencing Analysis Material and Methods
| Stage Name | Time | Description | Illustration |
|---|---|---|---|
Stage 1 | D0-D2 | Zygote and dispersed blastomere cleavage in a yolk syncytium. Days 0-2 post-egg capsule deposition at 20C. | |
Stage 2 | D2-D3 | Sphere formation. A fraction of the blastomeres differentiate into temporary embryonic tissues that provide form and function to the embryo. Undifferentiated blastomeres remain in the embryonic wall. Days 2-3 post-egg capsule deposition at 20C. |  |
Stage 3 | D2-D4 | Dispersed blastomere cleavage in the embryonic wall of nascent spheres. Yolk ingestion into the gut cavity. Days 2-4 post-egg capsule deposition at 20C. |  |
Stage 4 | D3-D5 | Dispersed blastomere cleavage in the embryonic wall. Yolk ingestion into the gut cavity. Early embryonic gut formation. Days 3-5 post-egg capsule deposition at 20C. |  |
Stage 5 | D5-D7 | Organogenesis onset. Downregulation of early embryo enriched transcripts and birth of adult lineages within the blastomere population. Early embryonic gut formation continues. Yolk ingestion into the gut cavity. Days 5-7 post-egg capsule deposition at 20C. |  |
Stage 6 | D7-D9 | Organogenesis and morphogenesis. Definitive organ formation. Temporary embryonic cell types degenerate. Embryo elongation. Days 7-9 post-egg capsule deposition at 20C. |  |
Stage 7 | D9-D11 | Organogenesis and organ maturation continues. Embryos elongated. Eyes just visible. Onset of gliding motility. Days 9-11 post-egg capsule deposition at 20C. |  |
Stage 8 | D13-D15 | Organogenesis and organ maturation continues. Hatching. Days 13-15 post-egg capsule deposition at 20C. |  |
