Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea

Overview
TitleMolecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea
AuthorsChong T, Stary JM, Wang Y, Newmark PA
TypeJournal Article
Journal NameBMC developmental biology
Volume11
IssueN/A
Year2011
Page(s)69
CitationChong T, Stary JM, Wang Y, Newmark PA. Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea. BMC developmental biology. 2011; 11:69.

Abstract

BACKGROUND
The freshwater planarian Schmidtea mediterranea exhibits two distinct reproductive modes. Individuals of the sexual strain are cross-fertilizing hermaphrodites with reproductive organs that develop post-embryonically. By contrast, individuals of the asexual strain reproduce exclusively by transverse fission and fail to develop reproductive organs. These different reproductive strains are associated with distinct karyotypes, making S. mediterranea a useful model for studying germline development and sexual differentiation.

RESULTS
To identify genes expressed differentially between these strains, we performed microarray analyses and identified >800 genes that were upregulated in the sexual planarian. From these, we characterized 24 genes by fluorescent in situ hybridization (FISH), revealing their expression in male germ cells or accessory reproductive organs. To identify additional markers of the planarian reproductive system, we also used immuno- and fluorescent lectin staining, identifying several antibodies and lectins that labeled structures associated with reproductive organs.

CONCLUSIONS
Collectively, these cell-type specific markers will enable future efforts to characterize genes that are important for reproductive development in the planarian.

Properties
Additional details for this publication include:
Property NameValue
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryEngland
DOI10.1186/1471-213X-11-69
Elocation10.1186/1471-213X-11-69
Publication ModelElectronic
ISSN1471-213X
eISSN1471-213X
Publication Date2011
Journal AbbreviationBMC Dev. Biol.
Publication TypeResearch Support, N.I.H., Extramural
Publication TypeResearch Support, Non-U.S. Gov't
Publication TypeResearch Support, U.S. Gov't, Non-P.H.S.
Cross References
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DatabaseAccession
PMID: PMID:22074376