Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain

Overview
TitleScale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain
AuthorsHama H, Kurokawa H, Kawano H, Ando R, Shimogori T, Noda H, Fukami K, Sakaue-Sawano A, Miyawaki A
TypeJournal Article
Journal NameNature neuroscience
Volume14
Issue11
Year2011
Page(s)1481-8
CitationHama H, Kurokawa H, Kawano H, Ando R, Shimogori T, Noda H, Fukami K, Sakaue-Sawano A, Miyawaki A. Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Nature neuroscience. 2011 Aug 30; 14(11):1481-8.

Abstract

Optical methods for viewing neuronal populations and projections in the intact mammalian brain are needed, but light scattering prevents imaging deep into brain structures. We imaged fixed brain tissue using Scale, an aqueous reagent that renders biological samples optically transparent but completely preserves fluorescent signals in the clarified structures. In Scale-treated mouse brain, neurons labeled with genetically encoded fluorescent proteins were visualized at an unprecedented depth in millimeter-scale networks and at subcellular resolution. The improved depth and scale of imaging permitted comprehensive three-dimensional reconstructions of cortical, callosal and hippocampal projections whose extent was limited only by the working distance of the objective lenses. In the intact neurogenic niche of the dentate gyrus, Scale allowed the quantitation of distances of neural stem cells to blood vessels. Our findings suggest that the Scale method will be useful for light microscopy-based connectomics of cellular networks in brain and other tissues.

Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic
ISSN1546-1726
eISSN1546-1726
Publication Date2011 Aug 30
Journal AbbreviationNat. Neurosci.
DOI10.1038/nn.2928
Elocation10.1038/nn.2928
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States
Publication TypeResearch Support, Non-U.S. Gov't
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PMID: PMID:21878933