An in situ hybridization protocol for planarian embryos: monitoring myosin heavy chain gene expression

Overview
TitleAn in situ hybridization protocol for planarian embryos: monitoring myosin heavy chain gene expression
AuthorsCardona A, Fernández J, Solana J, Romero R
TypeJournal Article
Journal NameDevelopment genes and evolution
Volume215
Issue9
Year2005
Page(s)482-88
CitationCardona A, Fernández J, Solana J, Romero R. An in situ hybridization protocol for planarian embryos: monitoring myosin heavy chain gene expression. Development genes and evolution. 2005 Sep; 215(9):482-88.

Abstract

The monitoring of gene expression is fundamental for understanding developmental biology. Here we report a successful experimental protocol for in situ hybridization in both whole-mount and sectioned planarian embryos. Conventional in situ hybridization techniques in developmental biology are used on whole-mount preparations. However, given that the inherent lack of external morphological markers in planarian embryos hinders the proper interpretation of gene expression data in whole-mount preparations, here we used sectioned material. We discuss the advantages of sectioned versus whole-mount preparations, namely, better probe penetration, improved tissue preservation, and the possibility to interpret gene expression in relation to internal morphological markers such as the epidermis, the embryonic and definitive pharynges, and the gastrodermis. Optimal fixatives and embedding methods for sectioning are also discussed.

Properties
Additional details for this publication include:
Property NameValue
Journal AbbreviationDev. Genes Evol.
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryGermany
Publication ModelPrint-Electronic
ISSN0949-944X
pISSN0949-944X
Publication Date2005 Sep
Publication TypeResearch Support, Non-U.S. Gov't
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DatabaseAccession
PMID: PMID:16010545