The Schmidtea mediterranea database as a molecular resource for studying platyhelminthes, stem cells and regeneration

Overview
TitleThe Schmidtea mediterranea database as a molecular resource for studying platyhelminthes, stem cells and regeneration
AuthorsSánchez Alvarado A, Newmark PA, Robb SM, Juste R
TypeJournal Article
Journal NameDevelopment (Cambridge, England)
Volume129
Issue24
Year2002
Page(s)5659-65
CitationSánchez Alvarado A, Newmark PA, Robb SM, Juste R. The Schmidtea mediterranea database as a molecular resource for studying platyhelminthes, stem cells and regeneration. Development (Cambridge, England). 2002 Dec; 129(24):5659-65.

Abstract

Platyhelminthes are excellent models for the study of stem cell biology, regeneration and the regulation of scale and proportion. In addition, parasitic forms infect millions of people worldwide. Therefore, it is puzzling that they remain relatively unexplored at the molecular level. We present the characterization of approximately 3,000 non-redundant cDNAs from a clonal line of the planarian Schmidtea mediterranea. The obtained cDNA sequences, homology comparisons and high-throughput whole-mount in situ hybridization data form part of the S. mediterranea database (SmedDb; http://planaria.neuro.utah.edu). Sixty-nine percent of the cDNAs analyzed share similarities with sequences deposited in GenBank and dbEST. The remaining gene transcripts failed to match sequences in other organisms, even though a large number of these (approximately 80%) contained putative open reading frames. Taken together, the molecular resources presented in this study, along with the ability of abrogating gene expression in planarians using RNA interference technology, pave the way for a systematic study of the remarkable biological properties displayed by Platyhelminthes.

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Additional details for this publication include:
Property NameValue
Journal CountryEngland
Publication ModelPrint
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
Journal AbbreviationDevelopment
Publication Date2002 Dec
pISSN0950-1991
ISSN0950-1991
Publication TypeResearch Support, Non-U.S. Gov't
Publication TypeResearch Support, U.S. Gov't, P.H.S.
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PMID: PubMedPMID:12421706