Germline Defects Caused by Smed-boule RNA-Interference Reveal That Egg Capsule Deposition Occurs Independently of Fertilization, Ovulation, Mating, or the Presence of Gametes in Planarian Flatworms

Overview
TitleGermline Defects Caused by Smed-boule RNA-Interference Reveal That Egg Capsule Deposition Occurs Independently of Fertilization, Ovulation, Mating, or the Presence of Gametes in Planarian Flatworms
AuthorsSteiner JK, Tasaki J, Rouhana L
TypeJournal Article
Journal NamePLoS genetics
Volume12
Issue5
Year2016
Page(s)e1006030
CitationSteiner JK, Tasaki J, Rouhana L. Germline Defects Caused by Smed-boule RNA-Interference Reveal That Egg Capsule Deposition Occurs Independently of Fertilization, Ovulation, Mating, or the Presence of Gametes in Planarian Flatworms. PLoS genetics. 2016 May; 12(5):e1006030.

Abstract

Few animals are known to lay eggs in the absence of ovulation or copulation, as it is presumably energetically wasteful and subjected to negative selection. Characterization of Smed-boule, a member of the DAZ family of germline RNA-binding proteins, revealed that egg capsule (or capsule) production and deposition occurs independently of the presence of gametes in the planarian flatworm Schmidtea mediterranea. Reduction of Smed-boule expression by RNA-interference (RNAi) causes ablation of spermatogonial stem cells and the inability of ovarian germline stem cells to undergo oogenesis. Although animals subjected to Smed-boule RNAi lose their gametes and become sterile, they continue to lay egg capsules. Production of sterile capsules is even observed in virgin Smed-boule(RNAi) and control planarians maintained in complete isolation, demonstrating that egg production in S. mediterranea occurs independently of ovulation, fertilization, or mating. Evidence suggests that this is a conserved feature amongst Platyhelminthes, and therefore relevant to the pathology and dissemination of parasitic flatworms. These findings demonstrate that Smed-boule functions at different stages during male and female germline stem cell development, and also demonstrate that egg capsule production by planarian flatworms occurs independently of signals produced by mating or ova.

Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
ISSN1553-7404
eISSN1553-7404
Publication Date2016 May
Journal AbbreviationPLoS Genet.
DOI10.1371/journal.pgen.1006030
Elocation10.1371/journal.pgen.1006030
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States
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PMID: PMID:27149082