40S ribosomal protein S20

Overview
Name40S ribosomal protein S20
Smed IDSMED30036013
Length (bp)466
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 40S ribosomal protein S20 (SMED30036013) t-SNE clustered cells

Violin plots show distribution of expression levels for 40S ribosomal protein S20 (SMED30036013) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 40S ribosomal protein S20 (SMED30036013) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 40S ribosomal protein S20 (SMED30036013) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30036013

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30036013SMESG000002673.1 SmedASXL_003092SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30036013SMESG000002673.1 dd_Smed_v4_123_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30036013SMESG000002673.1 dd_Smed_v4_123_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30036013SMESG000002673.1 dd_Smed_v4_123_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30036013SMESG000002673.1 dd_Smed_v4_123_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30036013SMESG000002673.1 dd_Smed_v4_123_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30036013SMESG000002673.1 dd_Smed_v4_123_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30036013SMESG000002673.1 dd_Smed_v4_123_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30036013SMESG000002673.1 dd_Smed_v4_123_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30036013SMESG000002673.1 dd_Smed_v4_123_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30036013SMESG000002673.1 dd_Smed_v4_123_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30036013SMESG000002673.1 SMESG000002662.1 Contig35333newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30036013SMESG000002673.1 SMESG000002662.1 Contig35333uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neoblastSMED30036013SMESG000002673.1 dd_Smed_v6_123_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30036013SMESG000002673.1 dd_Smed_v6_123_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 40S ribosomal protein S20 vs. Ensembl Human
Match: RPS20 (ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC:10405])

HSP 1 Score: 145.976 bits (367), Expect = 1.882e-45
Identity = 70/109 (64.22%), Postives = 87/109 (79.82%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE    + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   11 VEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. Ensembl Human
Match: RPS20 (ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC:10405])

HSP 1 Score: 145.976 bits (367), Expect = 1.882e-45
Identity = 70/109 (64.22%), Postives = 87/109 (79.82%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE    + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   11 VEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. Ensembl Human
Match: RPS20 (ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC:10405])

HSP 1 Score: 139.428 bits (350), Expect = 1.882e-42
Identity = 66/100 (66.00%), Postives = 81/100 (81.00%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSV 394
            VE    + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V
Sbjct:   11 VEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGV 110          
BLAST of 40S ribosomal protein S20 vs. Ensembl Human
Match: RPS20 (ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC:10405])

HSP 1 Score: 139.428 bits (350), Expect = 1.882e-42
Identity = 66/100 (66.00%), Postives = 81/100 (81.00%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSV 394
            VE    + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V
Sbjct:   11 VEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGV 110          
BLAST of 40S ribosomal protein S20 vs. Ensembl Human
Match: RPS20 (ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC:10405])

HSP 1 Score: 94.3597 bits (233), Expect = 9.652e-26
Identity = 42/64 (65.62%), Postives = 53/64 (82.81%), Query Frame = 2
Query:  230 MPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            MPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:    1 MPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 64          
BLAST of 40S ribosomal protein S20 vs. Ensembl Celegans
Match: rps-20 (Ribosomal Protein, Small subunit [Source:UniProtKB/TrEMBL;Acc:Q8WQA8])

HSP 1 Score: 129.798 bits (325), Expect = 1.557e-39
Identity = 58/100 (58.00%), Postives = 81/100 (81.00%), Query Frame = 2
Query:  119 RIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASE 418
            RIR+TLTS+ VKPLEKV   L++ AK +++ V+GP+RMPT+  RITTRKTPCGEGSKTWDR QM++HKR+++L    +++++I SI+IEP V  EVT ++
Sbjct:   18 RIRLTLTSQNVKPLEKVCAQLIDGAKNEHLIVKGPIRMPTKVLRITTRKTPCGEGSKTWDRFQMRIHKRLINLHAPAEVLRQITSISIEPGVDIEVTRAD 117          
BLAST of 40S ribosomal protein S20 vs. Ensembl Celegans
Match: rps-20 (Ribosomal Protein, Small subunit [Source:UniProtKB/TrEMBL;Acc:Q8WQA8])

HSP 1 Score: 129.798 bits (325), Expect = 1.557e-39
Identity = 58/100 (58.00%), Postives = 81/100 (81.00%), Query Frame = 2
Query:  119 RIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASE 418
            RIR+TLTS+ VKPLEKV   L++ AK +++ V+GP+RMPT+  RITTRKTPCGEGSKTWDR QM++HKR+++L    +++++I SI+IEP V  EVT ++
Sbjct:   18 RIRLTLTSQNVKPLEKVCAQLIDGAKNEHLIVKGPIRMPTKVLRITTRKTPCGEGSKTWDRFQMRIHKRLINLHAPAEVLRQITSISIEPGVDIEVTRAD 117          
BLAST of 40S ribosomal protein S20 vs. Ensembl Fly
Match: RpS20 (gene:FBgn0019936 transcript:FBtr0083964)

HSP 1 Score: 143.28 bits (360), Expect = 1.282e-44
Identity = 68/105 (64.76%), Postives = 83/105 (79.05%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVT 409
            V + A V RIRITLTS+ V+ LE V  DL+N AK +N++V+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV+KI SI IEP V  EVT
Sbjct:   13 VGDSASVHRIRITLTSRNVRSLENVCRDLINGAKNQNLRVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRIIDLHSPSEIVKKITSINIEPGVEVEVT 117          
BLAST of 40S ribosomal protein S20 vs. Ensembl Xenopus
Match: pdss2 (decaprenyl diphosphate synthase subunit 2 [Source:Xenbase;Acc:XB-GENE-945063])

HSP 1 Score: 146.362 bits (368), Expect = 1.243e-45
Identity = 71/108 (65.74%), Postives = 89/108 (82.41%), Query Frame = 2
Query:   98 EEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            +EVA + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   13 QEVA-IHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. Ensembl Mouse
Match: Rps20 (ribosomal protein S20 [Source:MGI Symbol;Acc:MGI:1914677])

HSP 1 Score: 145.976 bits (367), Expect = 1.292e-45
Identity = 70/109 (64.22%), Postives = 87/109 (79.82%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE    + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   11 VEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. UniProt/SwissProt
Match: sp|P60868|RS20_RAT (40S ribosomal protein S20 OS=Rattus norvegicus OX=10116 GN=Rps20 PE=3 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 9.040e-45
Identity = 70/109 (64.22%), Postives = 87/109 (79.82%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE    + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   11 VEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. UniProt/SwissProt
Match: sp|P60867|RS20_MOUSE (40S ribosomal protein S20 OS=Mus musculus OX=10090 GN=Rps20 PE=1 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 9.040e-45
Identity = 70/109 (64.22%), Postives = 87/109 (79.82%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE    + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   11 VEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. UniProt/SwissProt
Match: sp|Q4R5D0|RS20_MACFA (40S ribosomal protein S20 OS=Macaca fascicularis OX=9541 GN=RPS20 PE=3 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 9.040e-45
Identity = 70/109 (64.22%), Postives = 87/109 (79.82%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE    + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   11 VEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. UniProt/SwissProt
Match: sp|P60866|RS20_HUMAN (40S ribosomal protein S20 OS=Homo sapiens OX=9606 GN=RPS20 PE=1 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 9.040e-45
Identity = 70/109 (64.22%), Postives = 87/109 (79.82%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE    + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   11 VEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. UniProt/SwissProt
Match: sp|Q3ZBH8|RS20_BOVIN (40S ribosomal protein S20 OS=Bos taurus OX=9913 GN=RPS20 PE=3 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 9.040e-45
Identity = 70/109 (64.22%), Postives = 87/109 (79.82%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE    + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   11 VEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. TrEMBL
Match: A0A1B6ECP0 (Ribosomal_S10 domain-containing protein OS=Clastoptera arizonana OX=38151 GN=g.31555 PE=3 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 2.026e-43
Identity = 73/113 (64.60%), Postives = 87/113 (76.99%), Query Frame = 2
Query:   83 SDKKVEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            ++K   E AQV RIRITLTSK +K LEKV  DL+   K+KN+KV+GP+RMPT+  RITTRKTPCGEGSKTWDR QM+VHKRV+DL    DIVQ I SI IEP V  EVT +++
Sbjct:    8 TEKPTTETAQVHRIRITLTSKNIKSLEKVCADLMIEVKKKNLKVKGPIRMPTKVLRITTRKTPCGEGSKTWDRFQMRVHKRVIDLQSPSDIVQDITSINIEPGVEVEVTIADS 120          
BLAST of 40S ribosomal protein S20 vs. TrEMBL
Match: A0A1B6CVB7 (Ribosomal_S10 domain-containing protein OS=Clastoptera arizonana OX=38151 GN=g.31554 PE=3 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 2.048e-43
Identity = 72/112 (64.29%), Postives = 89/112 (79.46%), Query Frame = 2
Query:   86 DKKVEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            +K   E AQV RIRITLTSK +K LEKV  DL+  AK++N+KV+GPVRMPT+  RITTRKTPCGEGSKTWDR QM+VHKRV+DL    +IV++I SI+IEP V  EVT +++
Sbjct:    9 EKPTTETAQVHRIRITLTSKNMKSLEKVCADLIGGAKKQNLKVKGPVRMPTKVLRITTRKTPCGEGSKTWDRFQMRVHKRVIDLHSPSEIVKQITSISIEPGVEVEVTIADS 120          
BLAST of 40S ribosomal protein S20 vs. TrEMBL
Match: A0A1B6CA74 (Ribosomal_S10 domain-containing protein OS=Clastoptera arizonana OX=38151 GN=g.31556 PE=3 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 3.172e-43
Identity = 73/112 (65.18%), Postives = 86/112 (76.79%), Query Frame = 2
Query:   86 DKKVEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            +K   E AQV RIRITLTSK +K LEKV  DL+   K+KN+KV+GP+RMPT+  RITTRKTPCGEGSKTWDR QM+VHKRV+DL    DIVQ I SI IEP V  EVT +++
Sbjct:    9 EKPTTETAQVHRIRITLTSKNIKSLEKVCADLMIEVKKKNLKVKGPIRMPTKVLRITTRKTPCGEGSKTWDRFQMRVHKRVIDLQSPSDIVQDITSINIEPGVEVEVTIADS 120          
BLAST of 40S ribosomal protein S20 vs. TrEMBL
Match: Q6DJJ7 (MGC82136 protein OS=Xenopus laevis OX=8355 GN=MGC82136 PE=2 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 6.057e-43
Identity = 70/109 (64.22%), Postives = 88/109 (80.73%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE+   + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   11 VEQEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. TrEMBL
Match: A0A4W3J6B2 (Ribosomal_S10 domain-containing protein OS=Callorhinchus milii OX=7868 PE=3 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 6.611e-43
Identity = 70/109 (64.22%), Postives = 88/109 (80.73%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE+   + RIRITLTS+ VK LEKV  DL+  AK+KN+KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   11 VEQEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. Ensembl Cavefish
Match: RPS20 (ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC:10405])

HSP 1 Score: 144.436 bits (363), Expect = 4.409e-45
Identity = 72/117 (61.54%), Postives = 90/117 (76.92%), Query Frame = 2
Query:   80 MSD---KKVEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            MSD     VE    + RIRITLTS+ VK LEKV  DL+  AK+K++KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:    1 MSDTGKAPVETEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKSLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 117          
BLAST of 40S ribosomal protein S20 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005563.1 (pep scaffold:Pmarinus_7.0:GL480041:10158:12223:1 gene:ENSPMAG00000005038.1 transcript:ENSPMAT00000005563.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 136.732 bits (343), Expect = 1.140e-42
Identity = 64/109 (58.72%), Postives = 83/109 (76.15%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            V+    + RIRITLTS+    LEK+  DL+  AK KN++V+GPVRMPT+  RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   12 VDSEVSIHRIRITLTSRNAWYLEKICADLIRGAKDKNLRVKGPVRMPTKVLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 120          
BLAST of 40S ribosomal protein S20 vs. Ensembl Yeast
Match: RPS20 (Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 [Source:SGD;Acc:S000001007])

HSP 1 Score: 103.605 bits (257), Expect = 6.547e-30
Identity = 51/97 (52.58%), Postives = 70/97 (72.16%), Query Frame = 2
Query:  125 RITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTAS 415
            RITLTS +VK LE V +++V  A+Q N+  +GPVR+PT+  +I+TRKTP GEGSKTW+  +M++HKR +DL     IV++I  ITIEP V  EV  +
Sbjct:   23 RITLTSTKVKQLENVSSNIVKNAEQHNLVKKGPVRLPTKVLKISTRKTPNGEGSKTWETYEMRIHKRYIDLEAPVQIVKRITQITIEPGVDVEVVVA 119          
BLAST of 40S ribosomal protein S20 vs. Ensembl Nematostella
Match: EDO37788 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SES9])

HSP 1 Score: 144.821 bits (364), Expect = 1.013e-45
Identity = 69/108 (63.89%), Postives = 87/108 (80.56%), Query Frame = 2
Query:   98 EEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            EE AQ+ RIRITLTS+ VK LEKV  DL+  AK+K +KV+GPVRMPT+  RITTRKTPCGEGSKTWDR +M++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   12 EEEAQIHRIRITLTSRNVKSLEKVCADLIRGAKEKKLKVKGPVRMPTKFLRITTRKTPCGEGSKTWDRYEMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. Ensembl Nematostella
Match: EDO37787 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SES9])

HSP 1 Score: 144.821 bits (364), Expect = 1.013e-45
Identity = 69/108 (63.89%), Postives = 87/108 (80.56%), Query Frame = 2
Query:   98 EEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            EE AQ+ RIRITLTS+ VK LEKV  DL+  AK+K +KV+GPVRMPT+  RITTRKTPCGEGSKTWDR +M++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   12 EEEAQIHRIRITLTSRNVKSLEKVCADLIRGAKEKKLKVKGPVRMPTKFLRITTRKTPCGEGSKTWDRYEMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 119          
BLAST of 40S ribosomal protein S20 vs. Ensembl Medaka
Match: zgc:56231 (ribosomal protein S20 [Source:NCBI gene;Acc:101166754])

HSP 1 Score: 144.436 bits (363), Expect = 4.451e-45
Identity = 69/109 (63.30%), Postives = 87/109 (79.82%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE    + RIRITLTS+ VK LEKV  DL+  AK+K++KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   12 VESEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKSLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 120          
BLAST of 40S ribosomal protein S20 vs. Ensembl Medaka
Match: zgc:56231 (ribosomal protein S20 [Source:NCBI gene;Acc:101166754])

HSP 1 Score: 145.591 bits (366), Expect = 4.888e-45
Identity = 69/109 (63.30%), Postives = 87/109 (79.82%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEA 421
            VE    + RIRITLTS+ VK LEKV  DL+  AK+K++KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +IV++I SI+IEP V  EVT ++A
Sbjct:   45 VESEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKSLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA 153          
BLAST of 40S ribosomal protein S20 vs. Ensembl Medaka
Match: zgc:56231 (ribosomal protein S20 [Source:NCBI gene;Acc:101166754])

HSP 1 Score: 124.405 bits (311), Expect = 2.326e-33
Identity = 57/87 (65.52%), Postives = 70/87 (80.46%), Query Frame = 2
Query:   95 VEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDI 355
            VE    + RIRITLTS+ VK LEKV  DL+  AK+K++KV+GPVRMPT+T RITTRKTPCGEGSKTWDR QM++HKR++DL    +I
Sbjct:   11 VESEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKSLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEI 97          
BLAST of 40S ribosomal protein S20 vs. Planmine SMEST
Match: SMESG000002673.1 (SMESG000002673.1)

HSP 1 Score: 235.728 bits (600), Expect = 2.580e-81
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 2
Query:   80 MSDKKVEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEAVQE 430
            MSDKKVEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEAVQE
Sbjct:    1 MSDKKVEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVRMPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSITIEPSVYFEVTASEAVQE 117          
The following BLAST results are available for this feature:
BLAST of 40S ribosomal protein S20 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPS201.882e-4564.22ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS201.882e-4564.22ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS201.882e-4266.00ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS201.882e-4266.00ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS209.652e-2665.63ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of 40S ribosomal protein S20 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps-201.557e-3958.00Ribosomal Protein, Small subunit [Source:UniProtK... [more]
rps-201.557e-3958.00Ribosomal Protein, Small subunit [Source:UniProtK... [more]
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BLAST of 40S ribosomal protein S20 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
RpS201.282e-4464.76gene:FBgn0019936 transcript:FBtr0083964[more]
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BLAST of 40S ribosomal protein S20 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 40S ribosomal protein S20 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pdss21.243e-4565.74decaprenyl diphosphate synthase subunit 2 [Source:... [more]
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BLAST of 40S ribosomal protein S20 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Rps201.292e-4564.22ribosomal protein S20 [Source:MGI Symbol;Acc:MGI:1... [more]
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BLAST of 40S ribosomal protein S20 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P60868|RS20_RAT9.040e-4564.2240S ribosomal protein S20 OS=Rattus norvegicus OX=... [more]
sp|P60867|RS20_MOUSE9.040e-4564.2240S ribosomal protein S20 OS=Mus musculus OX=10090... [more]
sp|Q4R5D0|RS20_MACFA9.040e-4564.2240S ribosomal protein S20 OS=Macaca fascicularis O... [more]
sp|P60866|RS20_HUMAN9.040e-4564.2240S ribosomal protein S20 OS=Homo sapiens OX=9606 ... [more]
sp|Q3ZBH8|RS20_BOVIN9.040e-4564.2240S ribosomal protein S20 OS=Bos taurus OX=9913 GN... [more]
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BLAST of 40S ribosomal protein S20 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1B6ECP02.026e-4364.60Ribosomal_S10 domain-containing protein OS=Clastop... [more]
A0A1B6CVB72.048e-4364.29Ribosomal_S10 domain-containing protein OS=Clastop... [more]
A0A1B6CA743.172e-4365.18Ribosomal_S10 domain-containing protein OS=Clastop... [more]
Q6DJJ76.057e-4364.22MGC82136 protein OS=Xenopus laevis OX=8355 GN=MGC8... [more]
A0A4W3J6B26.611e-4364.22Ribosomal_S10 domain-containing protein OS=Callorh... [more]
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BLAST of 40S ribosomal protein S20 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
RPS204.409e-4561.54ribosomal protein S20 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of 40S ribosomal protein S20 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000005563.11.140e-4258.72pep scaffold:Pmarinus_7.0:GL480041:10158:12223:1 g... [more]
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BLAST of 40S ribosomal protein S20 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
RPS206.547e-3052.58Protein component of the small (40S) ribosomal sub... [more]
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BLAST of 40S ribosomal protein S20 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO377881.013e-4563.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377871.013e-4563.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 40S ribosomal protein S20 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
zgc:562314.451e-4563.30ribosomal protein S20 [Source:NCBI gene;Acc:101166... [more]
zgc:562314.888e-4563.30ribosomal protein S20 [Source:NCBI gene;Acc:101166... [more]
zgc:562312.326e-3365.52ribosomal protein S20 [Source:NCBI gene;Acc:101166... [more]
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BLAST of 40S ribosomal protein S20 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000002673.12.580e-81100.00SMESG000002673.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30036013 ID=SMED30036013|Name=40S ribosomal protein S20|organism=Schmidtea mediterranea sexual|type=transcript|length=466bp
ATTAGTTTTAAATATAAAACAAAATTTGTTTAAATTTTTGTTTAACAATT
TTATAAACTATTTTGAATAATATTAAACAATGTCTGACAAAAAAGTTGAA
GAAGTCGCACAAGTCGATAGAATTAGAATAACTCTTACTTCAAAAGAGGT
CAAACCATTGGAGAAAGTTGTTACTGATTTAGTTAACTTGGCTAAACAAA
AGAACATCAAAGTTAGAGGACCAGTTCGTATGCCAACTCAAACTAGAAGA
ATTACCACTCGTAAAACACCATGTGGTGAAGGTTCCAAAACTTGGGATCG
TCTTCAAATGAAAGTACACAAACGAGTTTTAGACTTGACTGGAGCCAAAG
ACATCGTTCAAAAGATCAACTCAATTACTATCGAACCTTCAGTTTATTTC
GAAGTAACTGCTTCTGAAGCTGTACAAGAATAAATGAAATAAATTTTTTC
TGGAATTAAAAAAAAA
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protein sequence of SMED30036013-orf-1

>SMED30036013-orf-1 ID=SMED30036013-orf-1|Name=SMED30036013-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=118bp
MSDKKVEEVAQVDRIRITLTSKEVKPLEKVVTDLVNLAKQKNIKVRGPVR
MPTQTRRITTRKTPCGEGSKTWDRLQMKVHKRVLDLTGAKDIVQKINSIT
IEPSVYFEVTASEAVQE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000039gamma neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR030395GP_PDE_dom
IPR001848Ribosomal_S10
IPR027486Ribosomal_S10_dom
IPR018268Ribosomal_S10_CS
Vocabulary: molecular function
TermDefinition
GO:0008081phosphoric diester hydrolase activity
GO:0003735structural constituent of ribosome
GO:0003723RNA binding
Vocabulary: biological process
TermDefinition
GO:0006629lipid metabolic process
GO:0006412translation
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0005622intracellular
GO:0005737cytoplasm
GO:0015935small ribosomal subunit
GO:0030529intracellular ribonucleoprotein complex