SMED30036011
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30036011 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30036011 aligns in the following genomic locations:
Homology
BLAST of SMED30036011 vs. Planmine SMEST
Match: SMESG000072549.1 (SMESG000072549.1) HSP 1 Score: 778.859 bits (2010), Expect = 0.000e+0 Identity = 433/443 (97.74%), Postives = 434/443 (97.97%), Query Frame = 2 Query: 2 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFYNEKLTIKLDPDRAVKGFHITCRNYTITLLNDIHDYEEDIFNKFQDILSYILLYDNQSIRFEYLLTPDVYQCGCKINSSAIMANEVKLDKLFQFDVRDKTFFPKGIWSKMHFYXXXXXXXXXXXXXXXXXXXAMIENYFMLLDDIHSKLFLLFGDIHVRTFNXXXXXXXXXISSDMNFISKMFHLYRLEHQEVSFHISFDSNYELLKHTLSNMYELNDAAYKAIRSKLMQRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLKLKPINPVLPKHSVSNKESEAGYLESKPATXXXXXXXXXXXXYYTFPSPDVKQTERRSEIRKSVRKSLKILDRNDIIRFQKFSVI 1330 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFYNEKLTIKLDPDRAVKGFHITCRNYTITLLNDIHDYEEDIFNKFQDILSYILLYDNQSIRFEYLLTPDVYQCGCKINSSAIMANEVKLDKLFQFDVRDKTFFPKGIWSKMHFYIILLEFGWLLLYLFLFIIFAMIENYF+LLDDIHSKLFLLFGDIHVRTFNKKYYYKCYYISSDMNFISKMFHLYRLEHQEVSFHISFDSNYELLKHTLSNMYELNDAAYKAIRSKLMQRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLKLKPI PVLPKHSVSNKESEAGYLESKPATMDMFSSGSFDMSYYTFPSP SEIRKSVRKSLKILDRNDIIRFQKFSVI Sbjct: 760 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFYNEKLTIKLDPDRAVKGFHITCRNYTITLLNDIHDYEEDIFNKFQDILSYILLYDNQSIRFEYLLTPDVYQCGCKINSSAIMANEVKLDKLFQFDVRDKTFFPKGIWSKMHFYIILLEFGWLLLYLFLFIIFAMIENYFLLLDDIHSKLFLLFGDIHVRTFNKKYYYKCYYISSDMNFISKMFHLYRLEHQEVSFHISFDSNYELLKHTLSNMYELNDAAYKAIRSKLMQRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLKLKPITPVLPKHSVSNKESEAGYLESKPATMDMFSSGSFDMSYYTFPSP--------SEIRKSVRKSLKILDRNDIIRFQKFSVI 1194
BLAST of SMED30036011 vs. Planmine SMEST
Match: SMESG000072549.1 (SMESG000072549.1) HSP 1 Score: 778.089 bits (2008), Expect = 0.000e+0 Identity = 433/443 (97.74%), Postives = 434/443 (97.97%), Query Frame = 2 Query: 2 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFYNEKLTIKLDPDRAVKGFHITCRNYTITLLNDIHDYEEDIFNKFQDILSYILLYDNQSIRFEYLLTPDVYQCGCKINSSAIMANEVKLDKLFQFDVRDKTFFPKGIWSKMHFYXXXXXXXXXXXXXXXXXXXAMIENYFMLLDDIHSKLFLLFGDIHVRTFNXXXXXXXXXISSDMNFISKMFHLYRLEHQEVSFHISFDSNYELLKHTLSNMYELNDAAYKAIRSKLMQRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLKLKPINPVLPKHSVSNKESEAGYLESKPATXXXXXXXXXXXXYYTFPSPDVKQTERRSEIRKSVRKSLKILDRNDIIRFQKFSVI 1330 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFYNEKLTIKLDPDRAVKGFHITCRNYTITLLNDIHDYEEDIFNKFQDILSYILLYDNQSIRFEYLLTPDVYQCGCKINSSAIMANEVKLDKLFQFDVRDKTFFPKGIWSKMHFYIILLEFGWLLLYLFLFIIFAMIENYF+LLDDIHSKLFLLFGDIHVRTFNKKYYYKCYYISSDMNFISKMFHLYRLEHQEVSFHISFDSNYELLKHTLSNMYELNDAAYKAIRSKLMQRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLKLKPI PVLPKHSVSNKESEAGYLESKPATMDMFSSGSFDMSYYTFPSP SEIRKSVRKSLKILDRNDIIRFQKFSVI Sbjct: 959 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFYNEKLTIKLDPDRAVKGFHITCRNYTITLLNDIHDYEEDIFNKFQDILSYILLYDNQSIRFEYLLTPDVYQCGCKINSSAIMANEVKLDKLFQFDVRDKTFFPKGIWSKMHFYIILLEFGWLLLYLFLFIIFAMIENYFLLLDDIHSKLFLLFGDIHVRTFNKKYYYKCYYISSDMNFISKMFHLYRLEHQEVSFHISFDSNYELLKHTLSNMYELNDAAYKAIRSKLMQRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLKLKPITPVLPKHSVSNKESEAGYLESKPATMDMFSSGSFDMSYYTFPSP--------SEIRKSVRKSLKILDRNDIIRFQKFSVI 1393
BLAST of SMED30036011 vs. Planmine SMEST
Match: SMESG000072549.1 (SMESG000072549.1) HSP 1 Score: 777.704 bits (2007), Expect = 0.000e+0 Identity = 433/443 (97.74%), Postives = 434/443 (97.97%), Query Frame = 2 Query: 2 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFYNEKLTIKLDPDRAVKGFHITCRNYTITLLNDIHDYEEDIFNKFQDILSYILLYDNQSIRFEYLLTPDVYQCGCKINSSAIMANEVKLDKLFQFDVRDKTFFPKGIWSKMHFYXXXXXXXXXXXXXXXXXXXAMIENYFMLLDDIHSKLFLLFGDIHVRTFNXXXXXXXXXISSDMNFISKMFHLYRLEHQEVSFHISFDSNYELLKHTLSNMYELNDAAYKAIRSKLMQRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLKLKPINPVLPKHSVSNKESEAGYLESKPATXXXXXXXXXXXXYYTFPSPDVKQTERRSEIRKSVRKSLKILDRNDIIRFQKFSVI 1330 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFYNEKLTIKLDPDRAVKGFHITCRNYTITLLNDIHDYEEDIFNKFQDILSYILLYDNQSIRFEYLLTPDVYQCGCKINSSAIMANEVKLDKLFQFDVRDKTFFPKGIWSKMHFYIILLEFGWLLLYLFLFIIFAMIENYF+LLDDIHSKLFLLFGDIHVRTFNKKYYYKCYYISSDMNFISKMFHLYRLEHQEVSFHISFDSNYELLKHTLSNMYELNDAAYKAIRSKLMQRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLKLKPI PVLPKHSVSNKESEAGYLESKPATMDMFSSGSFDMSYYTFPSP SEIRKSVRKSLKILDRNDIIRFQKFSVI Sbjct: 885 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFYNEKLTIKLDPDRAVKGFHITCRNYTITLLNDIHDYEEDIFNKFQDILSYILLYDNQSIRFEYLLTPDVYQCGCKINSSAIMANEVKLDKLFQFDVRDKTFFPKGIWSKMHFYIILLEFGWLLLYLFLFIIFAMIENYFLLLDDIHSKLFLLFGDIHVRTFNKKYYYKCYYISSDMNFISKMFHLYRLEHQEVSFHISFDSNYELLKHTLSNMYELNDAAYKAIRSKLMQRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLKLKPITPVLPKHSVSNKESEAGYLESKPATMDMFSSGSFDMSYYTFPSP--------SEIRKSVRKSLKILDRNDIIRFQKFSVI 1319
BLAST of SMED30036011 vs. Planmine SMEST
Match: SMESG000077924.1 (SMESG000077924.1) HSP 1 Score: 220.32 bits (560), Expect = 1.688e-65 Identity = 151/389 (38.82%), Postives = 226/389 (58.10%), Query Frame = 2 Query: 2 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFYNEKLTIKLDPDRAVKGFHITC-----------------RNYTITLLNDIHDYEEDIFNKFQDILSYILLYDNQSIRFEYLLTPDVYQCGCKINSSAIMANEVKLDKLFQFDVR--DKTFFPKGIWSKMHFYXXXXXXXXXXXXXXXXXXXAMIENYFMLLDDIHSKLFLLFGDIHVRTFNXXXXXXXXXISSDMNFISKMFHLYRLEHQEVSFHISF-----DSNYELLKHTLSNMYELNDAAYKAIRSKLM------QRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLK 1078 YRN+SV +Y++Q ++EPI++N+D+YYP+ + I+DF +NSLFFY T F+ + +EF++ L ++DG YQIF++YRS+ + E+LSYAFY+ KL I LDP R ++G++ITC NYTI LL+D +Y+ + + F D L+ ++LYDNQSI F Y + P +Y C C IN+ +I++ + + KLF DV+ D +FFPKG W++ H Y+ LL F WLL Y +F I A+I NY + L +I L+ ++G + + F K+YYK Y I+ D +F YR+ + SF S D N L+K +ELN+ + R KL QR L I + S E + +K RRK I D +L+ Sbjct: 110 YRNLSVPVYSIQRLTEPIIINMDLYYPIKILITDFISNSLFFYTTKTIFVTTLSKEFIVLNLLHTTVDGLYQIFFSYRSTEIKIHRSEILSYAFYSGKLMIILDPFRTLRGYYITCMTNSIDRLFFFEWYCFFENYTIKLLSDYKNYDPVLLDLFYDELTNLILYDNQSIYFGYSVKPAMYFCECVINTYSILSEPIVIRKLFPLDVKIFDMSFFPKGNWNQAHPYVFLLMFLWLLKYFLVFNILALINNYHIKLKEIDLNLYFMYGHLKTKCFTNKFYYKLYNITVDQDFYRHYMKKYRIIRKFSSFVTSTNPLVSDMNASLIKEKQFRRFELNEETFFGNRRKLRFHSTRKQRVLS-IDSGSVEIM-MDKIEDRRKLTIGKMDKSLQ 496
BLAST of SMED30036011 vs. Planmine SMEST
Match: SMESG000077924.1 (SMESG000077924.1) HSP 1 Score: 105.145 bits (261), Expect = 2.420e-24 Identity = 45/95 (47.37%), Postives = 71/95 (74.74%), Query Frame = 2 Query: 2 YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIKEIPREFVMFILHSNSMDGFYQIFYNYRSSSSHFLKRELLSYAFY 286 YRN+SV +Y++Q ++EPI++N+D+YYP+ + I+DF +NSLFFY T F+ + +EF++ L ++DG YQIF++YRS+ + E+LSYAFY Sbjct: 110 YRNLSVPVYSIQRLTEPIIINMDLYYPIKILITDFISNSLFFYTTKTIFVTTLSKEFIVLNLLHTTVDGLYQIFFSYRSTEIKIHRSEILSYAFY 204 HSP 2 Score: 50.447 bits (119), Expect = 1.636e-6 Identity = 64/185 (34.59%), Postives = 93/185 (50.27%), Query Frame = 2 Query: 557 FQFDVRDKTFFPKGIWSKMHFYXXXXXXXXXXXXXXXXXXXAMIENYFMLLDDIHSKLFLLFGDIHVRTFNXXXXXXXXXISSDMNFISKMFHLYRLEHQEVSFHISF-----DSNYELLKHTLSNMYELNDAAYKAIRSKLM------QRNLKRIGTYSFETLPFEKRAKRRKSKIKHKDTTLK 1078 + F + D +FFPKG W++ H Y+ LL F WLL Y +F I A+I NY + L +I L+ ++G + + F K+YYK Y I+ D +F YR+ + SF S D N L+K +ELN+ + R KL QR L I + S E + +K RRK I D +L+ Sbjct: 201 YAFYIFDMSFFPKGNWNQAHPYVFLLMFLWLLKYFLVFNILALINNYHIKLKEIDLNLYFMYGHLKTKCFTNKFYYKLYNITVDQDFYRHYMKKYRIIRKFSSFVTSTNPLVSDMNASLIKEKQFRRFELNEETFFGNRRKLRFHSTRKQRVLS-IDSGSVEIM-MDKIEDRRKLTIGKMDKSLQ 383 The following BLAST results are available for this feature:
BLAST of SMED30036011 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30036011 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30036011 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30036011 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30036011 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30036011 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30036011 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30036011 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30036011 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30036011 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30036011 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30036011 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30036011 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30036011 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30036011 ID=SMED30036011|Name=SMED30036011|organism=Schmidtea mediterranea sexual|type=transcript|length=1368bpback to top protein sequence of SMED30036011-orf-1 >SMED30036011-orf-1 ID=SMED30036011-orf-1|Name=SMED30036011-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=450bp YRNVSVYLYNVQSISEPIVVNIDMYYPLTLFISDFFTNSLFFYRASTTFIback to top |