SMED30036000
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30036000 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30036000 aligns in the following genomic locations:
Homology
BLAST of SMED30036000 vs. TrEMBL
Match: A0A0V1ANV1 (BED-type domain-containing protein (Fragment) OS=Trichinella spiralis OX=6334 GN=T01_9761 PE=4 SV=1) HSP 1 Score: 121.709 bits (304), Expect = 3.523e-26 Identity = 77/243 (31.69%), Postives = 129/243 (53.09%), Query Frame = -1 Query: 460 KLKEFQIDI*RDIISSTHDATAVIVKYGIILGITSQLCYNHGLHLAITDILYKKKPTTSFYE---------EDEEIPVYVEHA--VENSKDMTI-NSFGDANIVFGQHE*DYFLAKNNILQGYVKDKED*LLQLMLDMKIHWNSLIKMIKHFIQIKDLVNKALSDLGQ*KN--SDRHIAILKEF*DALLPLETAVKELGKNSATLLTAEGVYKFVFERLEECGMDLSKKLLKQIKNRINERRD 1146 KL++F++D+ DI++ D+ V+ K G +LGI QLCYN+G+HLA+ D++Y S ED++ Y+E+A ++N D + N + KN+ LQ +D L L++D ++ WNS++ ++ F+++KD V AL L N + + ++ + +AL P+ V LG+ +L TAE V +F+ L+E D++KKL +KNRI ERR+ Sbjct: 184 KLEQFELDMKTDIVALVTDSAFVMRKIGRLLGIEHQLCYNNGIHLAVVDVIYCVSNCASVESFNSSDDENCEDDDFDEYLENAPDIKNEFDAEVGNVVKKVRKIVASFR--RSPVKNDCLQKMCEDMLGKNLALIMDSRVRWNSMLTNMRRFLEMKDCVKSALKSLSDVDNFLYETELTLISDIVNALEPVAVCVNALGRKDCSLATAETVLEFLLRNLKEQRSDIAKKLFNAMKNRIEERRN 424
BLAST of SMED30036000 vs. TrEMBL
Match: A0A0J7KNG8 (Dimer_Tnp_hAT domain-containing protein OS=Lasius niger OX=67767 GN=RF55_8155 PE=4 SV=1) HSP 1 Score: 108.612 bits (270), Expect = 5.895e-22 Identity = 83/263 (31.56%), Postives = 132/263 (50.19%), Query Frame = -1 Query: 463 VENKLKEFQIDI*RDIISSTHDATAVIVKYGIILGITSQLCYNHGLHLAITDILYKKKPT-----------------------TSFYEEDEE-------IPVYVEHAVENSKDMTINSFGDANIVFGQHE*DYFLAKNN-ILQGYVKDKED*LLQLMLDMKIHWNSLIKMIKHFIQIKDLVNKALSDL-GQ*KNSDRHIAILKEF*DALLPLETAVKELGKNSATLLTAEGVYKFVFERLEECGMDLSKKLLKQIKNRINERR 1155 VE LK F I+I DI++ T D V++K G ++ Q C+ HG+ LAI DILYK P + ++D E IP V+ A N KD+ I +F + KN+ ILQ Y++++ D L+L+LD K WNSL+ M++ F ++ ++KAL DL + + ++ + + ++L P++ VK L + ATL+TAE +F+ E+L+ LS KL ++ RI +RR Sbjct: 119 VEEHLKIFNINIKDDIVAITADGPNVMIKVGNLMPCFQQRCFAHGIQLAIIDILYKDLPEEEAQDQEGLRRFQEIDNADQSELSDVDDDDNEGSFIVDCIPSSVKIA-SNYKDL-ITKVRKVVKIFRRSP-----TKNDLILQKYIQEEHDKRLELILDCKTRWNSLVNMLERFYIVRLCISKALIDLRSEIRFTETEWFAINDLKNSLQPVKLGVKVLCRRDATLITAETTVRFILEKLDNQSTPLSTKLAAALRRRIKQRR 374
BLAST of SMED30036000 vs. TrEMBL
Match: A0A4C1WY75 (Uncharacterized protein OS=Eumeta variegata OX=151549 GN=EVAR_35857_1 PE=4 SV=1) HSP 1 Score: 103.99 bits (258), Expect = 1.474e-20 Identity = 76/257 (29.57%), Postives = 125/257 (48.64%), Query Frame = -1 Query: 457 KLKEFQIDI*RDIISSTHDATAVIVKYGIILGITSQLCYNHGLHLAITDILYKKKPTTSFYEEDEEIPVYVEHAVENSKDMTINSFGDANIVFG--------QHE*DYFLAK--------------NNILQGYVKDKED*LLQLMLDMKIHWNSLIKMIKHFIQIKDLVNKALSDLGQ*KN-----SDRHIAILKEF*DALLPLETAVKELGKNSATLLTAEGVYKFVFERLEECGMDLSKKLLKQIKNRINERRDK 1146 KL EF + + DI+ T D +V+ K G ++ QLCY HG+ L ITD++YKK ++ EE E+ + + E + + N + F E ++K N++LQ Y++ + +QL+LD K W+S+ MI + ++K V K L DL N +D A+L + + L P++ AV+ L + L++AE +F+ +L LS+KL+ + NRI+ERR K Sbjct: 71 KLSEFDLSLDNDIVGITTDGASVMKKVGRLIEPLQQLCYAHGVQLGITDVIYKKTVQSNPEEEFEDPELNTNRSAEEEEVLGDNPDNYDGLYFSLPIRQVDLGTEYQMIISKLRKCVKLFKNSPTKNDMLQIYIQQEFGKTIQLVLDCKTRWSSICNMISTYNRVKQCVAKTLVDLSLSSNPEYFFTDEEHAVLIDLENTLQPVKLAVEVLCRQDIDLVSAEHTLRFMVRKLGNLKTPLSEKLVSSMNNRISERRIK 327
BLAST of SMED30036000 vs. TrEMBL
Match: A0A4Y2LA02 (Uncharacterized protein OS=Araneus ventricosus OX=182803 GN=AVEN_237838_1 PE=4 SV=1) HSP 1 Score: 100.523 bits (249), Expect = 2.796e-19 Identity = 79/238 (33.19%), Postives = 118/238 (49.58%), Query Frame = -1 Query: 532 VENKLKEFQIDI*RDIISSTHDATAVIVKYGIILGITSQLCYNHGLHLAITDILYKK--------KPTTS----------FYEEDEEIPVYVEHAVEN-----SKDMTINSFGDANIV------FGQHE*DYFLAKNNILQGYVKDKED*LLQLMLDMKIHWNSLIKMIKHFIQIKDLVNKALSDLGQ-*KNSDRHIAILKEF*DALLPLETAVKELGKNSATLLTAEGVYKFVFERL 1155 ++N+L+ F +D DII T D +V+VK G ++ QLC++HGLHLA+ DILYKK + TS + EE V V + S+ I + D V F + Y + LQ YVK+ L L+LD + WN L+ MI+ F ++K ++KAL D+G K SD +K+ D+L P + A++ L + +TLLTAE KF+ +L Sbjct: 208 IKNRLESFGLDSDTDIIGVTTDGASVMVKVGKLMSCYHQLCFSHGLHLAVVDILYKKNIEREEAHQEITSNESDTDDDDDTNDTHEEQGVTVTTTTDPRHLHLSRAEVIPRYNDLQKVRKVVKLFKRSPTKY----DTYLQKYVKEDTCKQLSLILDCRTRWNGLLAMIERFHKLKVCIDKALIDMGSDTKFSDLEWTKIKDLIDSLQPSKLAIEALCRRDSTLLTAETTLKFILGKL 441
BLAST of SMED30036000 vs. TrEMBL
Match: A0A4Y2BSA6 (Putative RNA-binding protein 19 OS=Araneus ventricosus OX=182803 GN=Rbm19 PE=4 SV=1) HSP 1 Score: 100.138 bits (248), Expect = 7.269e-19 Identity = 82/258 (31.78%), Postives = 128/258 (49.61%), Query Frame = -1 Query: 463 FQIDI*RDIISSTHDATAVIVKYGIILGITSQLCYNHGLHLAITDILYKKKPTTSFYEEDEEIPVYVEHAVENSKDM---------TINSFGD--------ANIV----------------FGQHE*DYFLAKNNILQGYVKDKED*LLQLMLDMKIHWNSLIKMIKHFIQIKDLVNKALSDLG-Q*KNSDRHIAILKEF*DALLPLETAVKELGKNSATLLTAEGVYKFVFERLEECGMDLSKKLLKQIKNRINERR 1134 F +D DII T D +V++K G ++ QLC+ HGL LA+ DILYKK + +E+E + + + +DM T+ + D A ++ F + Y + LQ YVK+ L L+LD WNSL+ MI+ F ++K ++KAL D+G + K S+ + +K+ D+L P + AV+ L + +TLLTAE KF+ E+L LS +L + ++ RI ERR Sbjct: 753 FSLDSDTDIIGVTTDGASVMIKVGKLMSCYHQLCFAHGLQLAMVDILYKK----NIEKEEEHQEITSNESDTDDEDMNDTHEEQGVTVTATTDPRHLHLTRAEVIPRYYDLLQKVRKVMKLFKRSPTKYDM----YLQKYVKEDTGKELSLILDCCTCWNSLLAMIERFHKLKVCIDKALIDIGSETKFSNLEWSKIKDLIDSLQPFKLAVEALCRRDSTLLTAETTLKFILEKLLTQDTVLSAQLSEVLRVRIKERR 1002
BLAST of SMED30036000 vs. Planmine SMEST
Match: SMESG000050481.1 (SMESG000050481.1) HSP 1 Score: 124.405 bits (311), Expect = 1.849e-32 Identity = 80/203 (39.41%), Postives = 114/203 (56.16%), Query Frame = -1 Query: 460 ILGITSQLCYNHGLHLAITDILYKKK---PTTSFYEEDEEIPVYVEHAVENSKDMTINSFGDANIVFGQHE*DYFLAKNNILQGYVKDKED*LLQLMLDMKIHWNSLIKMIKHFIQIKDLVNKALSDLGQ*KNSDRHIAILKEF*DALLPLETAVKELGKNSATLLTAEGVYKFVFERLEECGMDLSKKLLKQIKNRINERRD 1059 +LG+ +QLC+NHGLHLA+ D Y K P F + + I +E VE D T + + D N+ F + D + + K L+L LD+K WNSL MI F+ + + VNK+L +L + I L+ D L P+ETAV EL KN A L+TAEG YKF+FE+L+ +++KKL+ IK R++ERRD Sbjct: 4 LLGVLNQLCHNHGLHLAVMDCFYIPKTSIPNEVFDKTENFIDEGMEEEVEG--DSTDDQYSDYNLEFIEENEDAMTLNIDQTIKNLSTKIGEKLKLKLDIKTRWNSLSVMIGRFLILSECVNKSLLELNFDPIPENDIFELQNLVDFLAPVETAVTELSKNDANLITAEGAYKFLFEKLQPMQANIAKKLIHVIKKRMDERRD 204
BLAST of SMED30036000 vs. Planmine SMEST
Match: SMESG000052347.1 (SMESG000052347.1) HSP 1 Score: 117.472 bits (293), Expect = 2.328e-32 Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = -3 Query: 203 MTLLIFLETAAFIALVVEIFKRIHPPVILXXXXXXXXXXXXXXXXXXESLSSEVCNKLSLQKQLENAIASITHVEKQNEESCT 451 MTLLIFLETAA IALVVEIFKRIHPPVILNDDYEEITEEITIENEDEESLSSEVCNKLSLQKQLENAIASITHVEKQNEESCT Sbjct: 1 MTLLIFLETAASIALVVEIFKRIHPPVILNDDYEEITEEITIENEDEESLSSEVCNKLSLQKQLENAIASITHVEKQNEESCT 83
BLAST of SMED30036000 vs. Planmine SMEST
Match: SMESG000081415.1 (SMESG000081415.1) HSP 1 Score: 125.176 bits (313), Expect = 1.235e-31 Identity = 86/245 (35.10%), Postives = 135/245 (55.10%), Query Frame = -1 Query: 457 VENKLKEFQIDI*RDIISSTHDATAVIVKYGIILGITSQLCYNHGLHLAITDILYKKKPTTSFYEEDEEIPVYVEHAVENSKDMTINSF-GDANIVFGQH----E*DYF------LAKNNILQGYVKDKED*LLQLMLDMKIHWNSLIKMIKHFIQIKDLVNKALSDLGQ*K-NSDRHIAILKEF*DALLPLETAVKELGKNSATLLTAEGVYKFVFERLEECGMDLSKKLLKQIKNRINERRDK 1155 VE KL ++ I + R+I++ST D ++V+ KY + I +Q C NH +HLA+ + Y K + E +A + + NSF GD N+ E Y +N+ILQ YV+DKE L+L+LD + WNSLI M++ + ++ + AL ++G+ +++ IL + L P E AVKEL K+++TLLTAEGV F+F ++++ L KK +K RI+ERR+K Sbjct: 129 VEQKLMDYGIKL-RNILASTQDVSSVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDAISDSE-------NADGDEDENQFNSFAGDHNLNIDNDIDNTEYTYIRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIHMMERLVLLQVRIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNK 365
BLAST of SMED30036000 vs. Planmine SMEST
Match: SMESG000077975.1 (SMESG000077975.1) HSP 1 Score: 122.094 bits (305), Expect = 1.906e-30 Identity = 88/260 (33.85%), Postives = 137/260 (52.69%), Query Frame = -1 Query: 457 VENKLKEFQIDI*RDIISSTHDATAVIVKYGIILGITSQLCYNHGLHLAITDILYKKK--------PTTSFYEEDE----EIPVYVEHAVENSKDMT--INSFGDANI------------VFGQHE*DYFLAKNNILQGYVKDKED*LLQLMLDMKIHWNSLIKMIKHFIQIKDLVNKALSDLGQ*K-NSDRHIAILKEF*DALLPLETAVKELGKNSATLLTAEGVYKFVFERLEECGMDLSKKLLKQIKNRINERRDK 1155 VE KL ++ I + R+I++ST D +V+ KY + I +Q C NH +HLA+ + Y K + +EDE Y ++N D T S DANI F + +N+ILQ YV+DKE L+L+LD + WNS+I M++ + ++ + AL ++G+ +++ IL + L P E AVKEL K+++TLLTAEGV F+F ++++ L KK +K RI+ERR+K Sbjct: 195 VEQKLMDYGIKL-RNILTSTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGYHNLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSP-----VRNDILQRYVEDKEGKKLKLILDCRTRWNSIIPMMERLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNK 448
BLAST of SMED30036000 vs. Planmine SMEST
Match: SMESG000012010.1 (SMESG000012010.1) HSP 1 Score: 119.783 bits (299), Expect = 1.859e-29 Identity = 83/236 (35.17%), Postives = 125/236 (52.97%), Query Frame = -1 Query: 457 VENKLKEFQIDI*RDIISSTHDATAVIVKYGIILGITSQLCYNHGLHLAITDILYKKK---PTTSFYEEDEEIPVYVEHAVENSKDMTINSFGDANIVFGQHE*DYFLAKNNILQGYVKDKED*LLQLMLDMKIHWNSLIKMIKHFIQIKDLVNKALSDLGQ*KNSDRHIAILKEF*DALLPLETAVKELGKNSATLLTAEGVYKFVFERLEECGMDLSKKLLKQIKNRINERRDK 1155 VE KL +F+I I D+I ST+DA VI KY ILG+ +QLC+NH LHLA+ D Y K P F + + + +E VE D T + + D N+ F +++ ED ++ + I+++ IK + + VN +L +L + I L+ D L P+E AV EL +N + L+ AEGVYKF+FE+L+ +++KKL+ IK R+NER D Sbjct: 86 VEAKLTDFEIFIKNDVIVSTNDAATVIKKYDNILGVFNQLCHNHALHLAVMDCFYIPKTSIPNQVFDDTENFVDEGMEEEVEG--DSTEDQYSDYNLEF------------------IEENED-----VMTLNINFDQTIK------NLIECVNNSLLELNFDPIPENDIFALQNLVDVLAPVEIAVTELSQNDSNLIQAEGVYKFLFEKLQPIQTNIAKKLILTIKKRMNERIDP 290 HSP 2 Score: 65.855 bits (159), Expect = 1.683e-11 Identity = 32/70 (45.71%), Postives = 46/70 (65.71%), Query Frame = -1 Query: 457 DRHIAILKEF*DALLPLETAVKELGKNSATLLTAEGVYKFVFERLEECGMDLSKKLLKQIKNRINERRDK 666 + I L+ D L P+E AV EL +N + L+ AEGVYKF+FE+L+ +++KKL+ IK R+NER D Sbjct: 364 ENDIFALQNLVDVLAPVEIAVTELSQNDSNLIQAEGVYKFLFEKLQPIQTNIAKKLILTIKKRMNERIDP 433 The following BLAST results are available for this feature:
BLAST of SMED30036000 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30036000 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30036000 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30036000 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30036000 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30036000 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30036000 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30036000 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30036000 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30036000 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30036000 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30036000 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30036000 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30036000 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30036000 ID=SMED30036000|Name=SMED30036000|organism=Schmidtea mediterranea sexual|type=transcript|length=1173bpback to top protein sequence of SMED30036000-orf-1 >SMED30036000-orf-1 ID=SMED30036000-orf-1|Name=SMED30036000-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=134bp MTLLIFLETAAFIALVVEIFKRIHPPVILNDDYEEITEEITIENEDEESLback to top Annotated Terms
The following terms have been associated with this transcript:
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