SMED30035983

Overview
NameSMED30035983
Smed IDSMED30035983
Length (bp)1501
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30035983 (SMED30035983) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30035983 (SMED30035983) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30035983 (SMED30035983) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30035983 (SMED30035983) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30035983

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30035983SMESG000032618.1 SMESG000032616.1 SmedASXL_008392SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
neuronSMED30035983SMESG000032618.1 SMESG000032616.1 dd_Smed_v4_34828_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30035983SMESG000032618.1 SMESG000032616.1 dd_Smed_v6_34828_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30035983SMESG000032618.1 SMESG000032616.1 OX_Smed_1.0.00874ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30035983 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002254:40243..41744 -7493
v31.002254:49712..51212 +7299
Homology
BLAST of SMED30035983 vs. TrEMBL
Match: A0A1S6KMI4 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 621.698 bits (1602), Expect = 0.000e+0
Identity = 325/330 (98.48%), Postives = 327/330 (99.09%), Query Frame = 2
Query:  503 KAFRFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSGWPTWDNFVSVYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINMESTKRNPCLDVADNVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNPPTYEKDIYWSEIPTITEPYIRNYFYYPDCFKTTISAKLNCKIVKSNVLKFHINLIGEIKTXXXXXXXXIVNLLSDVXXXXXXXXXXXXXTIVEFLEYLAKVPRIIF 1492
            K  RFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSGWPTWDNFVSVYIHGKWQLPSS+TAGYAIPDTTGNAYIYLQKTFINMESTKRNPCL+VADNVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNPPTYEKDIYWSEIPTITEPYIRNYFYYPDCFKTTISAKLNCKIVKSNVLKFHINLIGEIKT SESSSYSIVNLLSDVGGILGLYGGSGCLTIVEFLEYLAKVPRIIF
Sbjct:   29 KHSRFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSGWPTWDNFVSVYIHGKWQLPSSTTAGYAIPDTTGNAYIYLQKTFINMESTKRNPCLNVADNVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNPPTYEKDIYWSEIPTITEPYIRNYFYYPDCFKTTISAKLNCKIVKSNVLKFHINLIGEIKTFSESSSYSIVNLLSDVGGILGLYGGSGCLTIVEFLEYLAKVPRIIF 358          

HSP 2 Score: 70.0922 bits (170), Expect = 2.867e-9
Identity = 31/31 (100.00%), Postives = 31/31 (100.00%), Query Frame = 3
Query:  420 MKSGAFVDPVLPVFKCVTYSDGNCRQILKHS 512
            MKSGAFVDPVLPVFKCVTYSDGNCRQILKHS
Sbjct:    1 MKSGAFVDPVLPVFKCVTYSDGNCRQILKHS 31          
BLAST of SMED30035983 vs. Planmine SMEST
Match: SMESG000032616.1 (SMESG000032616.1)

HSP 1 Score: 624.78 bits (1610), Expect = 0.000e+0
Identity = 335/376 (89.10%), Postives = 346/376 (92.02%), Query Frame = 2
Query:  371 NRPNQFIIAAITNTIRYEVRS--FCRPSVAGFQMCYIQ*WELPPNPKAFRFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSGWPTWDNFVSVYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINMESTKRNPCLDVADNVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNPPTYEKDIYWSEIPTITEPYIRNYFYYPDCFKTTISAKLNCKIVKSNVLKFHINLIGEIKTXXXXXXXXIVNLLSDVXXXXXXXXXXXXXTIVEFLEYLAKVPRIIF 1492
            N+ +Q  + ++ + I + ++S  F  P +  F+             K  RFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSGWPTWDNFVSVYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINMESTKRNPCLDVADNVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNPPTYEKDIYWSEIPTITEPYIRNYFYYPDCFKTTISAKLNCKIVKSNVLKFHINLIGEIKTSSESSSYSIVNLLSDVGGILGLYGGSGCLTIVEFLEYLAKVPRIIF
Sbjct:  128 NQTDQISLLSLQSLIPFGMKSGAFVDPVLPVFKCVTYSDGNCRQILKHSRFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSGWPTWDNFVSVYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINMESTKRNPCLDVADNVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNPPTYEKDIYWSEIPTITEPYIRNYFYYPDCFKTTISAKLNCKIVKSNVLKFHINLIGEIKTSSESSSYSIVNLLSDVGGILGLYGGSGCLTIVEFLEYLAKVPRIIF 503          

HSP 2 Score: 292.738 bits (748), Expect = 3.711e-93
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 3
Query:   54 AHGFIRLVLFKHRKIVITFWIVVIILSIVAFAYHLYVLSLRYTGVPLSTKLAFSPFNQDWPSVTICFDNPNLYGGNSTNYIPYYNNSFQNVSSLVREFWSSPYVNQTDXXXXXXXXXXXPFGMKSGAFVDPVLPVFKCVTYSDGNCRQILKHS 512
            AHGFIRLVLFKHRKIVITFWIVVIILSIVAFAYHLYVLSLRYTGVPLSTKLAFSPFNQDWPSVTICFDNPNLYGGNSTNYIPYYNNSFQNVSSLVREFWSSPYVNQTDQISLLSLQSLIPFGMKSGAFVDPVLPVFKCVTYSDGNCRQILKHS
Sbjct:   24 AHGFIRLVLFKHRKIVITFWIVVIILSIVAFAYHLYVLSLRYTGVPLSTKLAFSPFNQDWPSVTICFDNPNLYGGNSTNYIPYYNNSFQNVSSLVREFWSSPYVNQTDQISLLSLQSLIPFGMKSGAFVDPVLPVFKCVTYSDGNCRQILKHS 176          
BLAST of SMED30035983 vs. Planmine SMEST
Match: SMESG000032618.1 (SMESG000032618.1)

HSP 1 Score: 464.922 bits (1195), Expect = 1.707e-161
Identity = 230/275 (83.64%), Postives = 242/275 (88.00%), Query Frame = 2
Query:  371 NRPNQFIIAAITNTIRYEVRS--FCRPSVAGFQMCYIQ*WELPPNPKAFRFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSGWPTWDNFVSVYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINMESTKRNPCLDVADNVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNPPTYEK 1189
            N+ +Q  + ++ + I + ++S  F  P +  F+             K  RFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSGWPTWDNFVSVYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINMESTKRNPCLDVADNVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNP   +K
Sbjct:  128 NQTDQISLLSLQSLIPFGMKSGAFVDPVLPVFKCVTYSDGNCRQILKHSRFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSGWPTWDNFVSVYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINMESTKRNPCLDVADNVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNPQLMKK 402          

HSP 2 Score: 294.278 bits (752), Expect = 9.916e-95
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 3
Query:   54 AHGFIRLVLFKHRKIVITFWIVVIILSIVAFAYHLYVLSLRYTGVPLSTKLAFSPFNQDWPSVTICFDNPNLYGGNSTNYIPYYNNSFQNVSSLVREFWSSPYVNQTDXXXXXXXXXXXPFGMKSGAFVDPVLPVFKCVTYSDGNCRQILKHS 512
            AHGFIRLVLFKHRKIVITFWIVVIILSIVAFAYHLYVLSLRYTGVPLSTKLAFSPFNQDWPSVTICFDNPNLYGGNSTNYIPYYNNSFQNVSSLVREFWSSPYVNQTDQISLLSLQSLIPFGMKSGAFVDPVLPVFKCVTYSDGNCRQILKHS
Sbjct:   24 AHGFIRLVLFKHRKIVITFWIVVIILSIVAFAYHLYVLSLRYTGVPLSTKLAFSPFNQDWPSVTICFDNPNLYGGNSTNYIPYYNNSFQNVSSLVREFWSSPYVNQTDQISLLSLQSLIPFGMKSGAFVDPVLPVFKCVTYSDGNCRQILKHS 176          

HSP 3 Score: 70.0922 bits (170), Expect = 1.191e-12
Identity = 36/43 (83.72%), Postives = 36/43 (83.72%), Query Frame = 3
Query: 1146 GTIIFINSTPQLMKKTFIGLKSQLLQSHTFEIISIIPIALKLQ 1274
            G   F    PQLMKKTFIGLKSQLLQSHTFEIISIIPIALKLQ
Sbjct:  388 GNNYFYQFNPQLMKKTFIGLKSQLLQSHTFEIISIIPIALKLQ 430          
BLAST of SMED30035983 vs. Planmine SMEST
Match: SMESG000053471.1 (SMESG000053471.1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.374e-23
Identity = 49/156 (31.41%), Postives = 80/156 (51.28%), Query Frame = 3
Query:   54 AHGFIRLVLFKHRKIVITFWIVVIILSIVAFAYHLYVLSLRYTGVPLSTKLAFSPFNQDWPSVTICFDNPNLYGGNSTNYIPYYNN--SFQNVSSLVREFWSS-------PYVNQTDXXXXXXXXXXXPFGMKSGAFVDPVLPVFKCVTYSDGNCR 494
            AH + +L      K     WI ++ILS + F YH Y L+L++   P+STK+ +      +PS+TIC++ P  +  N +N +P +N+  +F  V  L  EFW         PY +    +  +   S+IP   K+   VDP +P+ KC   ++ +CR
Sbjct:   16 AHAYCKLTSHYKNKYRKGIWIFILILSCIIFIYHFYTLALKFMSNPISTKIKYDFQIDTYPSITICYNMP-FFIQNFSNPVPRFNSKMNFSVVDMLTMEFWDRNLNLINVPYGD----LFKIYFHSIIPLADKAAVLVDPRIPLVKCFMDNEYDCR 166          

HSP 2 Score: 53.1434 bits (126), Expect = 1.374e-23
Identity = 56/210 (26.67%), Postives = 94/210 (44.76%), Query Frame = 2
Query:  515 FVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSG-WPTWDNFVSVYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINME---STKRNPCLDVADNVEI-SDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCN 1129
            F+  N+ C +      N +   I S  I   W  S L    S  +  ++ F++VY+H     P+    G A+ +   N Y      F+N+    S  R  C DV +++++ S  + +I +  YS+ LC  I+ Q  ++  C CIS  L IP  F   +  N + +CH I   LL        +  +  ++ C++ +  N+   SC Q CN
Sbjct:  173 FIYENRPCIS-----GNVSMPKITSIKIAIFWPYSFLYPINSNPFINYNTFLAVYLHPLGTYPN----GLAL-EAVKNQY------FLNINYELSYNRLVCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLL--DASNSTVESIIERVKCHDKL-INNMSYSCPQKCN 363          
BLAST of SMED30035983 vs. Planmine SMEST
Match: SMESG000079503.1 (SMESG000079503.1)

HSP 1 Score: 53.1434 bits (126), Expect = 3.403e-7
Identity = 42/162 (25.93%), Postives = 78/162 (48.15%), Query Frame = 2
Query:  701 SSSTAGYAIPDTTGNAYIYLQKTFINMESTKRNPCLDVADNVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLL---------LNMTDFQHLNQLKAKLSCYENVYFNSPVD--SCIQYCNNAIYNGNN 1153
            S+  +G+    TT N  I +Q  F+      +N CL   + ++I  +   I   +Y++ LC M+  QN ++  C+C S  L IP  +S ++  +   FCH +    +         +N+ + +   QL +   C+++ + NS +D  SCI  C +  Y+ +N
Sbjct:  263 STRISGFKQFMTTINLNIRIQNEFL-----VKNSCLSNPEPMKILSYGNAILQFSYTKSLCLMVKKQNYIFQNCKCFSEALPIPN-YSDNITIDIVPFCHKLSKGFIEYLKTIPNPINILNNKETAQLTSSSVCHDH-FLNSWIDDGSCISPCTSKSYSWSN 417          
The following BLAST results are available for this feature:
BLAST of SMED30035983 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A1S6KMI42.867e-998.48Uncharacterized protein OS=Schmidtea mediterranea ... [more]
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BLAST of SMED30035983 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035983 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000032616.13.711e-9389.10SMESG000032616.1[more]
SMESG000032618.11.707e-16183.64SMESG000032618.1[more]
SMESG000053471.11.374e-2331.41SMESG000053471.1[more]
SMESG000079503.13.403e-725.93SMESG000079503.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035983 ID=SMED30035983|Name=SMED30035983|organism=Schmidtea mediterranea sexual|type=transcript|length=1501bp
GTAAAAGTGAATTATTGAAAGAAGAGCTTGTAGAATTTTTAGAAACGACA
ACTGCTCACGGATTCATTAGACTAGTTCTGTTTAAGCACAGGAAAATAGT
TATAACATTTTGGATTGTTGTCATAATACTTTCCATAGTGGCCTTTGCTT
ACCATTTATATGTTTTGTCTTTGAGATACACTGGAGTTCCGTTGAGCACC
AAATTAGCCTTTTCACCATTCAATCAAGATTGGCCGAGCGTAACAATTTG
CTTTGACAATCCCAATTTATATGGCGGCAATTCTACAAATTATATTCCAT
ATTATAACAATAGCTTTCAAAACGTGTCGAGTTTAGTCCGGGAATTTTGG
TCCAGTCCGTACGTAAATCAAACCGACCAAATCAGTTTATTATCGCTGCA
ATCACTAATACCATTCGGTATGAAGTCCGGAGCTTTTGTCGACCCAGTGT
TGCCGGTTTTCAAATGTGTTACATACAGTGATGGGAATTGCCGCCAAATC
CTAAAGCATTCAGATTCGTCATGAATAATAAAATTTGTGCAACAGTTGAT
TTACAAGAAAGTAACACTACAAATAATGTCATTCATTCATTTTCTATTAT
TTACTTATGGATGAGTTCAACTTTAAATACATACGTGTCAGGTTGGCCGA
CATGGGATAACTTTGTTTCGGTTTACATACACGGGAAATGGCAATTGCCA
TCATCTAGCACCGCCGGTTACGCAATTCCCGATACTACAGGAAATGCATA
CATATATTTGCAAAAAACCTTTATCAATATGGAATCTACAAAGAGAAATC
CTTGTTTGGACGTTGCTGACAATGTGGAAATTTCCGATTTCGACGGCCAA
ATTAGGAGCTTGAATTATTCCCAGCAACTCTGCAAAATGATCAACATCCA
AAATCAACTGTGGGATAAATGCCAATGTATCTCTTCGAATTTGTTGATTC
CAGGGCGATTTTCTCGCCATCTCAATGAGAATCAGAACATATTTTGCCAT
CACATTCACAGCAACCTCCTGTTGAATATGACAGATTTCCAGCATCTAAA
TCAGCTCAAGGCAAAATTGAGTTGCTATGAAAACGTGTATTTCAATAGTC
CCGTTGATTCGTGTATTCAATATTGTAATAATGCCATTTACAACGGGAAC
AATTATTTTTATCAATTCAACCCCCCAACTTATGAAAAAGACATTTATTG
GTCTGAAATCCCAACTATTACAGAGCCATACATTCGAAATTATTTCTATT
ATCCCGATTGCTTTAAAACTACAATAAGCGCAAAATTAAACTGTAAAATT
GTGAAAAGTAACGTTTTAAAGTTTCACATAAACTTAATAGGTGAAATCAA
AACATCTTCAGAATCCTCCAGTTACAGCATTGTGAATTTGCTGTCTGATG
TTGGCGGAATCCTGGGACTTTACGGAGGCAGCGGATGTCTGACCATTGTG
GAGTTTCTGGAATATCTCGCTAAAGTTCCCAGAATAATATTCTAATTACA
G
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protein sequence of SMED30035983-orf-1

>SMED30035983-orf-1 ID=SMED30035983-orf-1|Name=SMED30035983-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=174bp
KSELLKEELVEFLETTTAHGFIRLVLFKHRKIVITFWIVVIILSIVAFAY
HLYVLSLRYTGVPLSTKLAFSPFNQDWPSVTICFDNPNLYGGNSTNYIPY
YNNSFQNVSSLVREFWSSPYVNQTDQISLLSLQSLIPFGMKSGAFVDPVL
PVFKCVTYSDGNCRQILKHSDSS*
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protein sequence of SMED30035983-orf-2

>SMED30035983-orf-2 ID=SMED30035983-orf-2|Name=SMED30035983-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=325bp
MNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSGWPTWDNFVS
VYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINMESTKRNPCLDVAD
NVEISDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRH
LNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQ
YCNNAIYNGNNYFYQFNPPTYEKDIYWSEIPTITEPYIRNYFYYPDCFKT
TISAKLNCKIVKSNVLKFHINLIGEIKTSSESSSYSIVNLLSDVGGILGL
YGGSGCLTIVEFLEYLAKVPRIIF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR001873ENaC
Vocabulary: biological process
TermDefinition
GO:0006814sodium ion transport
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0005272sodium channel activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001873Epithelial sodium channelPFAMPF00858ASCcoord: 12..107
e-value: 1.7E-12
score: 47.0
NoneNo IPR availableGENE3DG3DSA:2.60.470.10coord: 64..85
e-value: 2.1E-5
score: 25.7
NoneNo IPR availableGENE3DG3DSA:1.10.287.770coord: 36..63
e-value: 2.1E-5
score: 25.7
NoneNo IPR availableGENE3DG3DSA:1.10.3590.10coord: 86..98
e-value: 2.1E-5
score: 25.7
NoneNo IPR availableTMHMMTMhelixcoord: 32..54