G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30035950
Length (bp)1889
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30035950) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30035950) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30035950) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30035950) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035950

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30035950SMESG000055047.1 dd_Smed_v4_15925_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30035950SMESG000055047.1 dd_Smed_v4_15925_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30035950SMESG000055047.1 dd_Smed_v4_15925_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30035950SMESG000055047.1 dd_Smed_v4_15925_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A267FQY4 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012459g3 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.473e-21
Identity = 88/367 (23.98%), Postives = 166/367 (45.23%), Query Frame = 3 
Query:  210 LYELFLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGF-CKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTSVFIVSTSVMCVYILHRTGIEKDRNEYFHNMDCTLTEKGYC-ECTKSK--------ISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFRKSFMEIFGFKKLFTSNFET--QLSSERKSS 1274
            LY+ F+P  ++ DR +T   +VLG+ GNT+S IVW  P +R   ++ +    +S+ DL +LLL   Q+ +  W  N+   + F C    VL  + ++ S LL +   ++  VS+  P   E   + +    +   + V  VL+++ +  F +          C +  +      +Y V   +V   L     +  ++M +  + R       +++           G   +C  +           N++    + T ++  + FF +  ++P  +   L+   N+G + +TD EVL DPVW+R+F +   K +  E+ L+ +SL  +I+L    QFR    +IF   +  +    +  +LS +R SS
Sbjct:   22 LYDGFIPNVMRFDRVVTPIWYVLGLFGNTLSAIVWLSPEVRRNNSSAVYLAALSLWDLAFLLLHTFQELKEAW--NISTQNTFTCSCLPVLLLTAQYLSPLLVLGFTVERWVSVCFPFRRESLCTPRRACSVVGAMTVGVVLWNLWQLYFKT---------GCEVAEE-RYGWTAYTVGTEVVISGLVPAAALVFNLMVLREIRRINSASAAHKFGAAAPSAADNGGGADQCGNASRDAGGGGAGGNTERKFRTTTVTLLCVSFFVILTTLPAGMVYGLQSYANFGSINMTDEEVLADPVWQRHFAMTRVKKVIDEVSLSHYSLNFLIYLATGMQFRLRVADIFRRIRRISCCCRSADELSGQRHSS 376
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A267DSG4 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012459g1 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 3.377e-21
Identity = 83/344 (24.13%), Postives = 156/344 (45.35%), Query Frame = 3 
Query:  210 LYELFLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGF-CKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTSVFIVSTSVMCVYILHRTGIEKDRNEYFHNMDCTLTEKGYC-ECTKSK--------ISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFRKSFMEIF 1211
            LY+ F+P  ++ DR +T   +VLG+ GNT+S IVW  P +R   ++ +    +S+ DL +LLL   Q+ +  W  N+   + F C    VL  + ++ S LL +   ++  VS+  P   E   + +    +   + V  VL+++ +  F +          C +  +      +Y V   +V   L     +  ++M +  + R       +++           G   +C  +           N++    + T ++  + FF +  ++P  +   L+   N+G + +TD EVL DPVW+R+F +   K +  E+ L+ +SL  +I+L    QFR    +IF
Sbjct:   22 LYDGFIPNVMRFDRVVTPIWYVLGLFGNTLSAIVWLSPEVRRNNSSAVYLAALSLWDLAFLLLHTFQELKEAW--NISTQNTFTCSCLPVLLLTAQYLSPLLVLGFTVERWVSVCFPFRRESLCTPRRACSVVGAMTVGVVLWNLWQLYFKT---------GCEVAEE-RYGWTAYTVGTEVVISGLVPAAALVFNLMVLREIRRINSASAAHKFGAAAPSAADNGGGAGQCGNASRDAGGGGAGGNTERKFRTTTVTLLCVSFFVILTTLPAGMVYGLQSYANFGSINMTDEEVLADPVWQRHFAMTRVKKVIDEVSLSHYSLNFLIYLATGMQFRLRVADIF 353
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A267FY03 (G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012459g2 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 5.041e-21
Identity = 83/344 (24.13%), Postives = 155/344 (45.06%), Query Frame = 3 
Query:  210 LYELFLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGF-CKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTSVFIVSTSVMCVYILHRTGIEKDRNEYFHNMDCTLTEKGYC-ECTKSK--------ISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFRKSFMEIF 1211
            LY+ F+P  ++ DR +T   +VLG+ GNT+S IVW  P +R   ++ +    +S+ DL +LLL   Q+ +  W  N+   + F C    VL  + ++ S LL +   ++  VS+  P   E   + +    +   + V  VL+++ +  F +          C +  +      +Y V   +V   L     +  ++M +  + R        ++           G   +C  +           N++    + T ++  + FF +  ++P  +   L+   N+G + +TD EVL DPVW+R+F +   K +  E+ L+ +SL  +I+L    QFR    +IF
Sbjct:   59 LYDGFIPNVMRFDRVVTPIWYVLGLFGNTLSAIVWLSPEVRRNNSSAVYLAALSLWDLAFLLLHTFQELKEAW--NISTQNTFTCSCLPVLLLTAQYLSPLLVLGFTVERWVSVCFPFRRELLCTPRRACSVVGAMTVGVVLWNLWQLYFKT---------GCEVAEE-RYGWTAYTVGTEVVISGLVPAAALVFNLMVLREIRRINSASAARKFGAAAPSVADNGGGAGQCGNASRDAGGGGAGGNTERKFRTTTVTLLCVSFFVILTTLPAGMVYGLQSYANFGSINMTDEEVLADPVWQRHFAMTRVKKVIDEVSLSHYSLNFLIYLATGMQFRLRVADIF 390
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0L8I0D0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22039887mg PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 4.278e-20
Identity = 80/339 (23.60%), Postives = 156/339 (46.02%), Query Frame = 3 
Query:  222 FLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGFCKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTSVFIVSTSVMCVYILHRTGIEKDRNEYFHNMDCTLTEKGYCECTKSKISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFRKSFMEIFGFKKLFTSN 1238
            ++P ++ +DRY T   +++G  GN +S IVW   R+R      L  I  ++ DLF+L L ++ + +  W   V      C  + VL  +C++   L  +   ++  +S+ +P   E Y + +   ++   L ++S   S  +G F   DS     K C  PR+  + +  + ++ W+V+F    +F++   ++ ++                N+   L  +     +K ++  +++   + T  +  + F+ +F  +P  +A SL     YG + +++ E   DP W+R+F     K I +E+ LT F     I+L   + FRK        +KL TS+
Sbjct:    3 YVPESIYVDRYTTPFLYIIGFPGNILSFIVWIQRRMRHSSGYYLAAI--ALADLFFLALQIVHEAQSAWFLTVLDYPIICGGYPVLFMTCQYLPPLFVLGFTVERYISVCHPFKRERYCTTRRAKIVILALIILSFSLSSMQGYFWHYDSA---CKSCS-PREWAINTSFWTIWTWIVEFL---IFMLVPGLVLIF----------------NILVILEVR---RMSKIELHGNEQRTSATTLMLLTVSFYLIFTVLPVTVATSLYTTFPYGKLGMSEEETRADPKWQRFFTYVFIKAIVNEIGLTHFVFNFFIYLITGRLFRKE------LEKLITSS 307
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: V4A7E3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_162598 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.301e-17
Identity = 85/382 (22.25%), Postives = 164/382 (42.93%), Query Frame = 3 
Query:  180 NVFKKHSTTSLYELFLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGFCKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKWTYLITFLLAVVSVLFSIAEG----VFSSIDSENIQMKECLLPRDILLTSKSYK-VYYWLVKFFLTSVFIVSTSVMCVYILHRTGIEKDRNEYFHNMDCTLTEKGYCECTKSKISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFRKSFMEI---FGFKKLFTSNFETQLSSERKSSLRQKSKPIS 1301
            +VF+++S   L + F P +    R +    +++G IGN IS ++W+  R+R   ++ +    +S+ D  +LLL ++      W    +  +  C++F  L    ++ S LL +   I+  +++ +P   E + + K   +I  +L + S++ + A+      +S  DS NI+      P+  +  + S+K ++ W  +  +    +V   V+   IL    I K  N                    S+ S       + T ++  + F+ +   +   +   LE L  +GD+ L+D E+  DP W + FN   A+ I   + L+ ++  I I+    K FRK  + I   +G               ER S +     P+S
Sbjct:   11 HVFQRYSIPQLLDTFAPGSPTAGRIVPPIWYMVGFIGNPISALIWFGKRMRRNNSSAIYLGALSISDFCFLLLHMLYILHSTWGYRTYNTESGCEVFNFLFYVPQYLSTLLVLGFTIERYIAVVHPFLKEKWCTVKRASIIVLVLTIFSMVIASAQTYIWYYYSVTDSCNIR------PQAQVGGTMSFKHIWTWFTEVLI--FILVPLLVLIFNILVLREIFKLSN----------------NGVISRQSGGSNSTTASTVTLLAVSFYLIVTQLTSTLVYCLEQLFEHGDIYLSDEEIRADPAWSKLFNYLTARKIIEVISLSHYACYIFIYALTGKHFRKEILYILTCYGRSSWLDKTCSKTHRGERYSMVSGNGNPMS 368
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000055047.1 (SMESG000055047.1)

HSP 1 Score: 1075.46 bits (2780), Expect = 0.000e+0
Identity = 554/560 (98.93%), Postives = 557/560 (99.46%), Query Frame = 3 
Query:  141 MTSVKKSAPFLEMNVFKKHSTTSLYELFLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGFCKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTSVFIVSTSVMCVYILHRTGIEKDRNEYFHNMDCTLTEKGYCECTKSKISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFRKSFMEIFGFKKLFTSNFETQLSSERKSSLRQKSKPISYYIDRSKNSYPFQKNQTLDKHLNNNYKINKFSNHSNPVSPRNVSSIKRVPKVQTLERRNARNQTCQKIEPIYYSVPWSTSSRHPSHYCRKTLPMNEMTTSIKTVRXXXXXXXXEFHNVCSEFEYYXXXXXXXXXXXPKMFVKNCHCNESPNSETDSDKFVLKYKKYGKRLHQV 1820
            MTS+KKSAPFLEMNVFKKHSTTSLYELFLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGFCKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTSVFIVSTSVMC+YILHRTGIEKDRNEYFHNMDCTLTEKGYCECTKSKISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGDVRLTDFEVLDDPVWERYFNIEGAK+IFHELYLTKFSLKIIIFLFCTKQFRKSFMEIFGFKKLFTSNFETQLSSERKSSLRQKSKPISYYIDRSKNSYPFQKNQTLDKHLNNNYKINKFSNHSNPVSPRNVSSIKRVPKVQTLERRNARNQTCQKIEPIYYSVPWSTSSRHPSHYCRKTLPMNEMTTSIKT RKPKKIPKKEFHNVCSEFEYYSPVASSSSD SPKMFVKNCHCNESPNSE DSDKFVLKYKKYGKRLHQV
Sbjct:    1 MTSIKKSAPFLEMNVFKKHSTTSLYELFLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGFCKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTSVFIVSTSVMCIYILHRTGIEKDRNEYFHNMDCTLTEKGYCECTKSKISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKVIFHELYLTKFSLKIIIFLFCTKQFRKSFMEIFGFKKLFTSNFETQLSSERKSSLRQKSKPISYYIDRSKNSYPFQKNQTLDKHLNNNYKINKFSNHSNPVSPRNVSSIKRVPKVQTLERRNARNQTCQKIEPIYYSVPWSTSSRHPSHYCRKTLPMNEMTTSIKTERKPKKIPKKEFHNVCSEFEYYSPVASSSSDGSPKMFVKNCHCNESPNSEADSDKFVLKYKKYGKRLHQV 560
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000031888.1 (SMESG000031888.1)

HSP 1 Score: 101.293 bits (251), Expect = 8.216e-23
Identity = 80/343 (23.32%), Postives = 161/343 (46.94%), Query Frame = 3 
Query:  198 STTSLYELFLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGFCKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTS---VFIVSTSVMCVYILHRTGIEKDRNEYFHNMDCTLTEKGYCECTK----SKISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFRKSFME 1205
            S+  L  +F P  + IDRY+TL   ++G IGN  S I+W  P++R + ++ +    +S+ D   L+  +IQD +  W+ NV +   FC I+ VL +  + +S +L +   I+  V++  P   +   + K   +++F L +  + ++I  G F  I +E  +   C+  +     +   K Y +  +   +    + I+  +++ +  ++R               C  T K    C +    ++  N++   L  T  +  + F+  F ++P  I  +L+ +    D  +   ++  DP W+ YF +   K +  EL ++ ++L  +I+L   K FRK F++
Sbjct:   10 SSIELNLMFSPNRIYIDRYMTLIWLLIGYIGNIFSFIIWIEPKMRRENSSAIYLAALSLNDFLVLVFVMIQDLQYSWNKNV-LRYSFCSIYMVLSTCSQLFSPVLVLAFTIERWVAICYPFKRQSLCTPKRAIIVSFTLYLFVLCYNIYNG-FMWIYNE--RFDSCVFNQLETNGTDFQKQYIFATEIVFSGCVPLLILGFNILVIKEMYRIT------------KCNRT-KSMGNCPQFHDPNQRFNNERSFLFTTIMLLAVSFYLFFTTLPGGIMFALQQVITMPDENMNLSKIPSDPKWQFYFRVSIIKAVIDELCISHYALNFVIYLVTGKAFRKQFID 335
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000031888.1 (SMESG000031888.1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.921e-18
Identity = 72/324 (22.22%), Postives = 150/324 (46.30%), Query Frame = 3 
Query:  255 LTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGFCKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTS---VFIVSTSVMCVYILHRTGIEKDRNEYFHNMDCTLTEKGYCECTK----SKISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFRKSFME 1205
            +TL   ++G IGN  S I+W  P++R + ++ +    +S+ D   L+  +IQD +  W+ NV +   FC I+ VL +  + +S +L +   I+  V++  P   +   + K   +++F L +  + ++I  G F  I +E  +   C+  +     +   K Y +  +   +    + I+  +++ +  ++R               C  T K    C +    ++  N++   L  T  +  + F+  F ++P  I  +L+ +    D  +   ++  DP W+ YF +   K +  EL ++ ++L  +I+L   K FRK F++
Sbjct:    1 MTLIWLLIGYIGNIFSFIIWIEPKMRRENSSAIYLAALSLNDFLVLVFVMIQDLQYSWNKNV-LRYSFCSIYMVLSTCSQLFSPVLVLAFTIERWVAICYPFKRQSLCTPKRAIIVSFTLYLFVLCYNIYNG-FMWIYNE--RFDSCVFNQLETNGTDFQKQYIFATEIVFSGCVPLLILGFNILVIKEMYRIT------------KCNRT-KSMGNCPQFHDPNQRFNNERSFLFTTIMLLAVSFYLFFTTLPGGIMFALQQVITMPDENMNLSKIPSDPKWQFYFRVSIIKAVIDELCISHYALNFVIYLVTGKAFRKQFID 307
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000018637.1 (SMESG000018637.1)

HSP 1 Score: 73.559 bits (179), Expect = 1.073e-13
Identity = 83/351 (23.65%), Postives = 154/351 (43.87%), Query Frame = 3 
Query:  216 ELFLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGFCKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEY--YKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTSVFIVSTSVMCVYILHRTGIEKDRNE----YFHNMDCTLTEKGYCECTKSKISNSDEDHLSL---TKSVTIIGFFAVFISIPQLIAVSL----EPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFRKSFMEIFGFKKLF 1229
            ++FLP + Q+  Y       +G++GN IS +VW   +LR   ++ +    +++ D F ++ ++I      W   +    G CK + V+ +  ++ SILL     I+  +++  P   E    ++R W       +A   +  S+   VF      + Q   C +P+  +    +Y V Y L    L  +F     V+ + +  R  +E  R       F  +               K S + E+ +S    T ++ ++ FF +  ++P L AV +     P  N   V+    ++ +DP+W+++F + GAK I   L L+ +   I+I+L  +  FR   M   GF KL+
Sbjct:   18 QIFLPISYQLQLYSIPIFLTVGILGNPISFVVWVSRKLRRNNSSAVYIAMLAINDFFVVICSIIYYLRHSWGFGIIDIRGICKFYMVIFTFLQYNSILLVFGFTIERWIAVCYPFRRERCCTENRAW-------IACGIIFSSVFISVFPLFFVWHYQ-PLCSVPKHWI--ENTYFVTYSLT---LEILFSALLPVITLAVNIRVVVEMYRISETIVTFPKLSAVNQNFEIPSQKSRKHSKNTENSISFKSTTITLLLVSFFQILTTLP-LGAVYICQIFYPEGNATSVK----QMQNDPIWKKHFQMHGAKDIIDLLSLSHYCFNIVIYLASSHLFRNHLM---GFVKLY 347
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000018637.1 (SMESG000018637.1)

HSP 1 Score: 73.559 bits (179), Expect = 1.264e-13
Identity = 83/351 (23.65%), Postives = 154/351 (43.87%), Query Frame = 3 
Query:  216 ELFLPTALQIDRYLTLTSFVLGVIGNTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNVFINDGFCKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEY--YKSRKWTYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKVYYWLVKFFLTSVFIVSTSVMCVYILHRTGIEKDRNE----YFHNMDCTLTEKGYCECTKSKISNSDEDHLSL---TKSVTIIGFFAVFISIPQLIAVSL----EPLNNYGDVRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFRKSFMEIFGFKKLF 1229
            ++FLP + Q+  Y       +G++GN IS +VW   +LR   ++ +    +++ D F ++ ++I      W   +    G CK + V+ +  ++ SILL     I+  +++  P   E    ++R W       +A   +  S+   VF      + Q   C +P+  +    +Y V Y L    L  +F     V+ + +  R  +E  R       F  +               K S + E+ +S    T ++ ++ FF +  ++P L AV +     P  N   V+    ++ +DP+W+++F + GAK I   L L+ +   I+I+L  +  FR   M   GF KL+
Sbjct:   63 QIFLPISYQLQLYSIPIFLTVGILGNPISFVVWVSRKLRRNNSSAVYIAMLAINDFFVVICSIIYYLRHSWGFGIIDIRGICKFYMVIFTFLQYNSILLVFGFTIERWIAVCYPFRRERCCTENRAW-------IACGIIFSSVFISVFPLFFVWHYQ-PLCSVPKHWI--ENTYFVTYSLT---LEILFSALLPVITLAVNIRVVVEMYRISETIVTFPKLSAVNQNFEIPSQKSRKHSKNTENSISFKSTTITLLLVSFFQILTTLP-LGAVYICQIFYPEGNATSVK----QMQNDPIWKKHFQMHGAKDIIDLLSLSHYCFNIVIYLASSHLFRNHLM---GFVKLY 392
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267FQY42.473e-2123.98G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A267DSG43.377e-2124.13G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A267FY035.041e-2124.13G_PROTEIN_RECEP_F1_2 domain-containing protein (Fr... [more]
A0A0L8I0D04.278e-2023.60G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
V4A7E31.301e-1722.25G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000055047.10.000e+098.93SMESG000055047.1[more]
SMESG000031888.18.216e-2323.32SMESG000031888.1[more]
SMESG000031888.12.921e-1822.22SMESG000031888.1[more]
SMESG000018637.11.073e-1323.65SMESG000018637.1[more]
SMESG000018637.11.264e-1323.65SMESG000018637.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035950 ID=SMED30035950|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1889bp
TTATACCAAAATGGTAAAAGAACACAATTCCGTCCATAAAATTATTCATG
CTGTTACTATTAGTGTTATGCTAATGAAGGTTCATATTTTCAGCATAAAA
TTATTTATTCATTTTAAGCGTTAACATTGAATATTTGCATATGACTAGTG
TAAAGAAATCTGCCCCCTTTCTAGAGATGAATGTTTTTAAAAAACATTCA
ACGACATCTCTTTATGAGCTTTTCCTTCCAACAGCTCTGCAAATAGATCG
GTACCTTACCCTCACCTCATTCGTACTGGGAGTTATAGGTAACACGATCT
CAATCATTGTCTGGTGGCATCCCAGATTAAGAATAAAAGAAGCCGCCGTT
TTGACTTTTATTTTTATTTCAGTTTTAGATTTGTTCTATTTGCTGTTGAC
ATTGATTCAGGATTTTGAGCGAGTTTGGTCGACAAATGTATTCATAAATG
ATGGATTTTGTAAGATTTTTAAAGTCCTCCAAAGCAGTTGTGAGTTCTAT
TCAATTCTCCTGACCATGCTCGTCTGTATACAGCACATTGTGTCCCTGAA
GAATCCCGACTCCTATGAATATTACAAATCAAGAAAGTGGACTTATTTAA
TAACTTTTTTATTGGCAGTAGTTTCGGTTTTATTTTCAATTGCTGAAGGA
GTTTTCTCATCAATAGACAGCGAGAACATCCAAATGAAAGAATGTTTACT
TCCTAGGGATATTTTGTTGACAAGCAAAAGTTACAAGGTTTATTATTGGT
TGGTGAAATTCTTTTTGACTTCAGTATTCATTGTCTCAACATCGGTAATG
TGCGTATACATTCTCCATAGGACCGGAATAGAAAAGGATAGAAATGAGTA
TTTCCATAATATGGATTGCACTTTAACCGAAAAGGGTTATTGTGAATGCA
CCAAGAGTAAGATCTCGAATTCGGATGAAGATCATTTATCTCTGACAAAG
TCTGTAACAATAATCGGATTCTTTGCCGTCTTCATCAGCATACCTCAACT
GATTGCAGTCAGTCTGGAGCCACTTAATAATTATGGGGATGTGAGATTGA
CAGATTTTGAGGTACTGGACGACCCCGTATGGGAACGCTATTTTAACATC
GAAGGTGCAAAAATAATATTTCATGAGTTGTACTTAACCAAATTTTCACT
AAAGATTATAATTTTTTTATTTTGTACAAAACAGTTCCGTAAAAGTTTTA
TGGAAATATTTGGCTTTAAAAAGTTGTTTACGTCTAATTTTGAAACTCAA
TTATCGTCTGAAAGAAAGTCAAGTTTACGCCAAAAATCAAAGCCAATCTC
ATACTACATCGATAGAAGCAAGAATTCCTATCCATTCCAAAAGAATCAAA
CACTTGACAAACATTTAAACAATAATTACAAAATCAACAAATTCTCAAAT
CATTCAAATCCAGTTTCTCCAAGGAATGTTAGCAGTATCAAAAGAGTTCC
AAAAGTTCAAACCCTTGAAAGACGAAATGCAAGAAATCAAACATGTCAGA
AAATCGAGCCGATTTATTATTCCGTTCCTTGGTCAACTTCATCGAGACAT
CCTTCGCATTATTGTAGAAAGACTCTACCAATGAATGAGATGACCACAAG
CATCAAAACCGTAAGGAAACCGAAAAAAATTCCAAAGAAAGAATTTCATA
ATGTTTGCAGTGAATTTGAATATTACAGTCCAGTTGCATCAAGTTCTTCC
GATGAGTCGCCAAAAATGTTCGTAAAGAACTGCCATTGTAATGAAAGTCC
GAATTCGGAAACGGATTCCGATAAATTTGTACTGAAATATAAAAAATATG
GAAAACGATTGCATCAAGTTTAATATGGAAATGGAAATTTTTCACGGTTT
TGATCTGATTTATTGCACTCATGTTCTTTATAAATAATA
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protein sequence of SMED30035950-orf-1

>SMED30035950-orf-1 ID=SMED30035950-orf-1|Name=SMED30035950-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=561bp
MTSVKKSAPFLEMNVFKKHSTTSLYELFLPTALQIDRYLTLTSFVLGVIG
NTISIIVWWHPRLRIKEAAVLTFIFISVLDLFYLLLTLIQDFERVWSTNV
FINDGFCKIFKVLQSSCEFYSILLTMLVCIQHIVSLKNPDSYEYYKSRKW
TYLITFLLAVVSVLFSIAEGVFSSIDSENIQMKECLLPRDILLTSKSYKV
YYWLVKFFLTSVFIVSTSVMCVYILHRTGIEKDRNEYFHNMDCTLTEKGY
CECTKSKISNSDEDHLSLTKSVTIIGFFAVFISIPQLIAVSLEPLNNYGD
VRLTDFEVLDDPVWERYFNIEGAKIIFHELYLTKFSLKIIIFLFCTKQFR
KSFMEIFGFKKLFTSNFETQLSSERKSSLRQKSKPISYYIDRSKNSYPFQ
KNQTLDKHLNNNYKINKFSNHSNPVSPRNVSSIKRVPKVQTLERRNARNQ
TCQKIEPIYYSVPWSTSSRHPSHYCRKTLPMNEMTTSIKTVRKPKKIPKK
EFHNVCSEFEYYSPVASSSSDESPKMFVKNCHCNESPNSETDSDKFVLKY
KKYGKRLHQV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 76..295
e-value: 3.3E-8
score: 33.2
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 32..363
e-value: 4.2E-25
score: 90.3
NoneNo IPR availablePANTHERPTHR24229NEUROPEPTIDES RECEPTORcoord: 39..386
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 35..353
e-value: 3.08891E-9
score: 56.102
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 28..375
NoneNo IPR availableTMHMMTMhelixcoord: 266..288
NoneNo IPR availableTMHMMTMhelixcoord: 70..89
NoneNo IPR availableTMHMMTMhelixcoord: 150..172
NoneNo IPR availableTMHMMTMhelixcoord: 38..60
NoneNo IPR availableTMHMMTMhelixcoord: 203..225
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 50..225
score: 12.267