Tubulin beta chain

Overview
NameTubulin beta chain
Smed IDSMED30035933
Length (bp)1388
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tubulin beta chain (SMED30035933) t-SNE clustered cells

Violin plots show distribution of expression levels for Tubulin beta chain (SMED30035933) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30035933

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testisSMED30035933h1SMcG0016933 Contig4330_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30035933h1SMcG0016933 Contig4330_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30035933h1SMcG0016933 Contig4330_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile stagecDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB2A (tubulin beta 2A class IIa [Source:HGNC Symbol;Acc:HGNC:12412])

HSP 1 Score: 698.738 bits (1802), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEA G + VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKESE+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET++IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB2B (tubulin beta 2B class IIb [Source:HGNC Symbol;Acc:HGNC:30829])

HSP 1 Score: 698.738 bits (1802), Expect = 0.000e+0
Identity = 333/410 (81.22%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATG + VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKESE+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB4B (tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:HGNC:20771])

HSP 1 Score: 697.197 bits (1798), Expect = 0.000e+0
Identity = 331/410 (80.73%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 696.427 bits (1796), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 378/410 (92.20%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQL+RI+VYYNEATGG+ VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQ+FD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMAVTFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 696.427 bits (1796), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 378/410 (92.20%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQL+RI+VYYNEATGG+ VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQ+FD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMAVTFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-4 (Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot;Acc:P41937])

HSP 1 Score: 697.582 bits (1799), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEA+GG+ VPR  L DLEP TMDSV+ GP+G LFRPDNF +G +GAGNNWAKGHYT+GAEL+D+VL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DETF IDNEALYDICFRTLKL+ PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR+ +V +LTQQMFD KN+MAACDPRHG+YLTVAAMFRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TF+GN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGAYNGDSDLQLERINVYYNEASGGKYVPRACLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESCDCLQGFQMTHSLGGGTGSGMGTLLISKIREEYPDRIMMTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSMTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGVKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: ben-1 (Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q18817])

HSP 1 Score: 697.197 bits (1798), Expect = 0.000e+0
Identity = 326/410 (79.51%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+  DG YKG+SDLQLERINVYYNEA GG+ VPR +L DLEP TMDSV+ GP+G LFRPDNF +G +GAGNNWAKGHYT+GAEL+D+VL++VRKE+E C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ +FS  PSPKVSD VVEPYNATL++HQLVE  DETF IDNEALYDICFRTLKLSNPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPL+++ +Q YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAAMFRGR+S++EVD++M+NVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHVQAGQCGNQIGAKFWEVISDEHGIQPDGTYKGESDLQLERINVYYNEANGGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLSNPTYGDLNHLVSVTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGAQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDDQMMNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-1 (Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:O17921])

HSP 1 Score: 687.567 bits (1773), Expect = 0.000e+0
Identity = 320/410 (78.05%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+G+KFWEVISDEHG+  DG +KG+SDLQLERI+VYYNEA  G+ VPR +L DLEP TMDSV+ GP+G LFRPDNF +G +GAGNNWAKGHYT+GAEL+D+VL+++RKE+E C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREEFPDRI+ +FS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDIC+RTLKL+NPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPL+++ +Q YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAAMFRGR+S++EVDE+ML+VQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TF+GN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGESDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGAQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-1 (Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:O17921])

HSP 1 Score: 687.567 bits (1773), Expect = 0.000e+0
Identity = 320/410 (78.05%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+G+KFWEVISDEHG+  DG +KG+SDLQLERI+VYYNEA  G+ VPR +L DLEP TMDSV+ GP+G LFRPDNF +G +GAGNNWAKGHYT+GAEL+D+VL+++RKE+E C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREEFPDRI+ +FS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDIC+RTLKL+NPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPL+++ +Q YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAAMFRGR+S++EVDE+ML+VQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TF+GN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGESDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGAQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-2 (Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P52275])

HSP 1 Score: 687.182 bits (1772), Expect = 0.000e+0
Identity = 319/410 (77.80%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+G+KFWEVISDEHG+  DG +KG++DLQLERI+VYYNEA  G+ VPR +L DLEP TMDSV+ GP+G LFRPDNF +G +GAGNNWAKGHYT+GAEL+D+VL+++RKE+E C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ +FS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDIC+RTLKL+NPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPL+++ +Q YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAAMFRGR+S++EVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TF+GN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGTQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub56D (gene:FBgn0284243 transcript:FBtr0086536)

HSP 1 Score: 694.886 bits (1792), Expect = 0.000e+0
Identity = 327/410 (79.76%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWE+ISDEHG+   G Y GDSDLQLERINVYYNEA+GG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ T+S  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLN+QNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub85D (gene:FBgn0003889 transcript:FBtr0082046)

HSP 1 Score: 686.411 bits (1770), Expect = 0.000e+0
Identity = 326/410 (79.51%), Postives = 371/410 (90.49%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+G KFWEVISDEH +   G Y GDSDLQLERINVYYNEATG + VPR IL DLEP TMDSV+ G +G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKESE C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLN+QNKNS++FVEWIP+N KTAVCDIPPRG+KM+ TFIGN+TAIQE+FKR++EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGGKFWEVISDEHCIDATGTYYGDSDLQLERINVYYNEATGAKYVPRAILVDLEPGTMDSVRSGAFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSFFVEWIPNNCKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0343443)

HSP 1 Score: 678.707 bits (1750), Expect = 0.000e+0
Identity = 325/415 (78.31%), Postives = 374/415 (90.12%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEAT-----GGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV +Q GQCGNQ+GAKFWE+IS+EHG+  +G Y GDSDLQLER++VYYNEA+     GG+ VPR IL DLEP TM+SV+ GPYG LFRPDNF YG +GAGNNWAKGHYT+GAEL+D+VL++VRKE E C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ T+S  PSPKVSD VVEPYNATL+IHQLVE  DET+ IDNEALYDICFRTLK+SNP+YGDLNHLVS TMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+ML VQNKNS+YFVEWIP+NVKTAVCDIPP+G+KM+ TFIGN TAIQE+FKRI+EQ++AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDSNGIYVGDSDLQLERVSVYYNEASVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKVSNPSYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLAVQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTE 415          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0072270)

HSP 1 Score: 678.322 bits (1749), Expect = 0.000e+0
Identity = 325/416 (78.12%), Postives = 374/416 (89.90%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEAT------GGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV +Q GQCGNQ+GAKFWE+IS+EHG+  +G Y GDSDLQLER++VYYNEA+      GG+ VPR IL DLEP TM+SV+ GPYG LFRPDNF YG +GAGNNWAKGHYT+GAEL+D+VL++VRKE E C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ T+S  PSPKVSD VVEPYNATL+IHQLVE  DET+ IDNEALYDICFRTLK+SNP+YGDLNHLVS TMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+ML VQNKNS+YFVEWIP+NVKTAVCDIPP+G+KM+ TFIGN TAIQE+FKRI+EQ++AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDSNGIYVGDSDLQLERVSVYYNEASAVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKVSNPSYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLAVQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTE 416          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub97EF (gene:FBgn0003890 transcript:FBtr0300611)

HSP 1 Score: 670.618 bits (1729), Expect = 0.000e+0
Identity = 318/410 (77.56%), Postives = 367/410 (89.51%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+G+KFWE+ISDEHG+  +G+Y G+S LQ ERI+VYYNEA+ G+ VPR +L DLEP TMDSV+  P G LFRPDNF YG +GAGNNWAKGHYT+GAELIDSVLE++RKESE C+CLQGFQ+ HSLGGGTGSG+GTLL+SKIREE+PDRI+ +FS  PSPKVSD VVEPYNATL+IHQLVE  DETF IDNEALYDICFRTLKLS+PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLT++ SQ YR  +V +LTQQMFD KN+M ACDPRHG+YLTVA +FRG +S+KEVD +M NVQ+KNS+YFVEWIP+NVK AVCDIPPRG+KM+ TFIGN+TAIQEIFKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEIISDEHGIDPNGYYHGESALQHERIDVYYNEASSGKYVPRAVLIDLEPGTMDSVRQSPVGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELIDSVLEVLRKESEGCDCLQGFQLAHSLGGGTGSGLGTLLISKIREEYPDRIMNSFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETFCIDNEALYDICFRTLKLSSPTYGDLNHLVSVTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTAKGSQQYRALTVAELTQQMFDAKNMMTACDPRHGRYLTVACIFRGPMSMKEVDTQMYNVQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMSATFIGNSTAIQEIFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 699.123 bits (1803), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 699.123 bits (1803), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 699.123 bits (1803), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 699.123 bits (1803), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:153426 (zgc:153426 [Source:ZFIN;Acc:ZDB-GENE-060929-220])

HSP 1 Score: 698.353 bits (1801), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 378/410 (92.20%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQL+RI+VYYNEATGG+ VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR+ SV +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLSVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: znf330 (zinc finger protein 330 [Source:Xenbase;Acc:XB-GENE-1010548])

HSP 1 Score: 698.353 bits (1801), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATG + VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: reps2 (RALBP1 associated Eps domain containing 2 [Source:Xenbase;Acc:XB-GENE-985000])

HSP 1 Score: 698.353 bits (1801), Expect = 0.000e+0
Identity = 331/410 (80.73%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: ubxn11 (UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-985480])

HSP 1 Score: 697.967 bits (1800), Expect = 0.000e+0
Identity = 331/410 (80.73%), Postives = 378/410 (92.20%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEA+GG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSV+++VRKESE+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR+ +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVMDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDSKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb (tubulin beta class I [Source:NCBI gene;Acc:448742])

HSP 1 Score: 696.427 bits (1796), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 378/410 (92.20%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQL+RI+VYYNEATGG+ VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQ+FD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMAVTFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb3 (tubulin beta 3 class III [Source:NCBI gene;Acc:395045])

HSP 1 Score: 669.848 bits (1727), Expect = 0.000e+0
Identity = 322/410 (78.54%), Postives = 368/410 (89.76%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERI+VYYNEA+  + VPR IL DLEP TMDSV+ G +G LFRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE E C+CLQGFQ+ HSLGGGTGSGMGTLL+SK+REE+PDRI+ TFS  PSPKVSD VVEPYNATL+IHQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTT LRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLT+R SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVA +FRGR+S+KEVDE+ML +Q+KNS+YFVEWIP+NVK AVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPCGNYIGDSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2b (tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:MGI:1920960])

HSP 1 Score: 698.738 bits (1802), Expect = 0.000e+0
Identity = 333/410 (81.22%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATG + VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKESE+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2a (tubulin, beta 2A class IIA [Source:MGI Symbol;Acc:MGI:107861])

HSP 1 Score: 698.738 bits (1802), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEA G + VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKESE+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET++IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4b (tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:MGI:1915472])

HSP 1 Score: 697.197 bits (1798), Expect = 0.000e+0
Identity = 331/410 (80.73%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb5 (tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI:107812])

HSP 1 Score: 696.427 bits (1796), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 378/410 (92.20%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQL+RI+VYYNEATGG+ VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQ+FD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMAVTFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4a (tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:MGI:107848])

HSP 1 Score: 694.115 bits (1790), Expect = 0.000e+0
Identity = 330/410 (80.49%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG  VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+D+VL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREEFPDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+ML+VQ+KNS+YFVEWIP+NVKTAVCDIPPRG+KMA TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|Q9NFZ6|TBB2_ECHMU (Tubulin beta-2 chain OS=Echinococcus multilocularis OX=6211 GN=TUB-2 PE=3 SV=1)

HSP 1 Score: 700.664 bits (1807), Expect = 0.000e+0
Identity = 330/410 (80.49%), Postives = 378/410 (92.20%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEA+GG+ VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL+++RKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ T+S  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  SV +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TF+GN+TAIQE+F+R++EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRAGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVIRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMVTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFVGNSTAIQELFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|O44388|TBB_TRITR (Tubulin beta chain OS=Trichuris trichiura OX=36087 PE=3 SV=1)

HSP 1 Score: 700.279 bits (1806), Expect = 0.000e+0
Identity = 334/410 (81.46%), Postives = 378/410 (92.20%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEA+GG+ VPR IL DLEP TMDSV+ GP+G+LFRPDNF +G +GAGNNWAKGHYT+GAEL+D+VL++VRKESE+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DETF IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKN+AYFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRAGPFGTLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQGYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNNAYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P30883|TBB4_XENLA (Tubulin beta-4 chain OS=Xenopus laevis OX=8355 GN=tubb4 PE=2 SV=1)

HSP 1 Score: 698.738 bits (1802), Expect = 0.000e+0
Identity = 331/410 (80.73%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P32882|TBB2_CHICK (Tubulin beta-2 chain OS=Gallus gallus OX=9031 PE=1 SV=1)

HSP 1 Score: 698.738 bits (1802), Expect = 0.000e+0
Identity = 333/410 (81.22%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATG + VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKESE+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|Q3KRE8|TBB2B_RAT (Tubulin beta-2B chain OS=Rattus norvegicus OX=10116 GN=Tubb2b PE=1 SV=1)

HSP 1 Score: 698.738 bits (1802), Expect = 0.000e+0
Identity = 333/410 (81.22%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATG + VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKESE+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A419PIP4 (Tubulin beta chain OS=Clonorchis sinensis OX=79923 GN=tbb-4 PE=3 SV=1)

HSP 1 Score: 724.161 bits (1868), Expect = 0.000e+0
Identity = 342/410 (83.41%), Postives = 385/410 (93.90%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ+GQCGNQMGAKFWEVISDEHG++  GFY+GD+DLQLERI VYYNEA GG+ VPR+IL DLEP TMD+V+CGP G LFRPDN+ +G TGAGNNWAKGHYTDGAELID+VLE+VRKE+E CEC+QGFQVVH+LGGGTG+GMGTLL++K+REEFPDR+VC+FST PSPKVSDVVVEPYNATL+IHQLVE  DETF IDNEALYDIC RTLKLS PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL TN++PFPRLHFF+PGF+PLTSRHS PYR+ +V DLT QMFD KN+MAACDPRHGKYLTVAAMFRGR+S+KEVDE+MLNVQN+NSAYFVEWIP+NVKTAVCDIPPRG+KM+VTFIGNNTAIQEIFKR+ EQ+AAM+RR+AFLHWYTGEGMDEMEF+E
Sbjct:    1 MREIVHIQIGQCGNQMGAKFWEVISDEHGITNTGFYQGDTDLQLERICVYYNEAIGGKFVPRSILCDLEPGTMDAVRCGPVGQLFRPDNYVFGSTGAGNNWAKGHYTDGAELIDNVLEVVRKEAELCECMQGFQVVHALGGGTGAGMGTLLINKVREEFPDRVVCSFSTVPSPKVSDVVVEPYNATLSIHQLVENCDETFCIDNEALYDICNRTLKLSTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLATNMVPFPRLHFFMPGFSPLTSRHSMPYRSNTVQDLTHQMFDSKNMMAACDPRHGKYLTVAAMFRGRMSMKEVDEQMLNVQNRNSAYFVEWIPNNVKTAVCDIPPRGLKMSVTFIGNNTAIQEIFKRVGEQFAAMFRRRAFLHWYTGEGMDEMEFSE 410          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A0V0J3W3 (Tubulin beta chain OS=Schistocephalus solidus OX=70667 GN=TR83001 PE=3 SV=1)

HSP 1 Score: 724.161 bits (1868), Expect = 0.000e+0
Identity = 346/410 (84.39%), Postives = 384/410 (93.66%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQVGQCGNQMGAKFWEV+SDEHG+S +G YKGDSDLQLERI+VYYNEATGG+ VPR ILTDLEP TMD+V+ GP G+LFRPDNF +G TGAGNNWAKGHYTDGAELIDSV E+VRKE+E  ECLQGFQ+ H+LGGGTG+GMGTLL+SKIREEFPDRI+CTFST PSPKVSDVVVEPYNATL++HQLVE +DETF IDNEALYDIC RTLKL +PTYGDLNHLVSAT+SG+TTCLRFPGQLNADLRKL TN++PFPRLHFF+PGF+PLTSR+SQ YRTYSV DLT QMFD KN+MAACDPRHGKYLTVAAMFRGR+S+KEVDE MLNVQNKNSAYFVEWIP+NVKTAVCDIPPRG+KMAVTFIGNNTAIQ IFKR+ EQ+AAM++R+AFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQVGQCGNQMGAKFWEVVSDEHGISPEGLYKGDSDLQLERISVYYNEATGGKFVPRAILTDLEPGTMDAVRSGPVGNLFRPDNFVFGATGAGNNWAKGHYTDGAELIDSVTEVVRKEAEYSECLQGFQITHALGGGTGAGMGTLLISKIREEFPDRIICTFSTVPSPKVSDVVVEPYNATLSVHQLVENSDETFCIDNEALYDICNRTLKLVSPTYGDLNHLVSATVSGITTCLRFPGQLNADLRKLATNMVPFPRLHFFMPGFSPLTSRYSQAYRTYSVQDLTHQMFDFKNMMAACDPRHGKYLTVAAMFRGRISMKEVDENMLNVQNKNSAYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNNTAIQHIFKRVGEQFAAMFKRRAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A4S2LW18 (Tubulin beta chain OS=Opisthorchis felineus OX=147828 GN=CRM22_004465 PE=3 SV=1)

HSP 1 Score: 723.005 bits (1865), Expect = 0.000e+0
Identity = 342/410 (83.41%), Postives = 385/410 (93.90%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ+GQCGNQMGAKFWEVISDEHG++  GFY+GD+DLQLERI VYYNEA GG+ VPR+IL DLEP TMD+V+CGP G LFRPDN+ +G TGAGNNWAKGHYTDGAELID+VLE+VRKE+E CEC+QGFQVVH+LGGGTG+GMGTLL++K+REEFPDR+VC+FST PSPKVSDVVVEPYNATL+IHQLVE  DETF IDNEALYDIC RTLKLS PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL TN++PFPRLHFF+PGF+PLTSRHS PYR+ +V DLT QMFD KN+MAACDPRHGKYLTVAAMFRGR+S+KEVDE+MLNVQN+NSAYFVEWIP+NVKTAVCDIPPRG+KM+VTFIGNNTAIQEIFKR+ EQ+AAM+RR+AFLHWYTGEGMDEMEF+E
Sbjct:    1 MREIVHIQIGQCGNQMGAKFWEVISDEHGITNTGFYQGDTDLQLERICVYYNEAIGGKFVPRSILCDLEPGTMDAVRCGPVGQLFRPDNYVFGSTGAGNNWAKGHYTDGAELIDNVLEVVRKEAELCECMQGFQVVHALGGGTGAGMGTLLINKVREEFPDRVVCSFSTVPSPKVSDVVVEPYNATLSIHQLVENCDETFCIDNEALYDICNRTLKLSTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLATNMVPFPRLHFFMPGFSPLTSRHSMPYRSNTVQDLTHQMFDNKNMMAACDPRHGKYLTVAAMFRGRMSMKEVDEQMLNVQNRNSAYFVEWIPNNVKTAVCDIPPRGLKMSVTFIGNNTAIQEIFKRVGEQFAAMFRRRAFLHWYTGEGMDEMEFSE 410          
BLAST of Tubulin beta chain vs. TrEMBL
Match: I6TL06 (Tubulin beta chain OS=Spirometra erinaceieuropaei OX=99802 PE=2 SV=1)

HSP 1 Score: 722.62 bits (1864), Expect = 0.000e+0
Identity = 344/410 (83.90%), Postives = 384/410 (93.66%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQVGQCGNQMGAKFWEV+SDEHG+S +G YKGDSDLQLERI+VYYNEATGG+ VPR +LTDLEP TMD+V+ GP G+LFRPDNF +G TGAGNNWAKGHYTDGAELIDSV E+VRKE+E  ECLQGFQ+ H+LGGGTG+GMGTLL+SKIREEFPDRI+C+FST PSPKVSDVVVEPYNATL++HQLVE +DETF IDNEALYDIC RTLKL +PTYGDLNHLVSAT+SG+TTCLRFPGQLNADLRKL TN++PFPRLHFF+PGF+PLTSR+SQ YRTYSV DLT QMFD KN+MAACDPRHGKYLTVAAMFRGR+S+KEVDE MLNVQNKNSAYFVEWIP+NVKTAVCDIPPRG+KMAVTFIGNNTAIQ IFKR+ EQ+AAM++R+AFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQVGQCGNQMGAKFWEVVSDEHGISPEGLYKGDSDLQLERISVYYNEATGGKFVPRAVLTDLEPGTMDAVRSGPVGNLFRPDNFVFGATGAGNNWAKGHYTDGAELIDSVTEVVRKEAEYSECLQGFQITHALGGGTGAGMGTLLISKIREEFPDRIICSFSTVPSPKVSDVVVEPYNATLSVHQLVENSDETFCIDNEALYDICNRTLKLVSPTYGDLNHLVSATVSGITTCLRFPGQLNADLRKLATNMVPFPRLHFFMPGFSPLTSRYSQAYRTYSVQDLTHQMFDFKNMMAACDPRHGKYLTVAAMFRGRISMKEVDENMLNVQNKNSAYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNNTAIQHIFKRVGEQFAAMFKRRAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A074ZDG4 (Tubulin beta chain OS=Opisthorchis viverrini OX=6198 GN=T265_07310 PE=3 SV=1)

HSP 1 Score: 721.079 bits (1860), Expect = 0.000e+0
Identity = 341/410 (83.17%), Postives = 384/410 (93.66%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ+GQCGNQMGAKFWEVISDEHG++  GFY+GD+DLQLERI VYYNEA GG+ VPR+IL DLEP TMD+V+CGP G LFRPDN+ +G TGAGNNWAKGHYTDGAELID+VLE+VRKE+E CEC+QGFQVVH+LGGGTG+GMGTLL++K+REEFPDR+VC+FST PSPKVSDVVVEPYNATL+IHQLVE  DETF IDNEALYDIC RTLKLS PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL TN++PFPRLHFF+PGF+PLTSRHS PYR+ +V DLT QMFD KN+MAACDPRHGKYLTVAAMFRGR+S+KEVDE+MLNVQN+NSAYFVEWIP+NVKTAVCDIPPRG+KM+VTFIGNNTAIQEIFKR+ EQ+ AM+RR+AFLHWYTGEGMDEMEF+E
Sbjct:    1 MREIVHIQIGQCGNQMGAKFWEVISDEHGITNTGFYQGDTDLQLERICVYYNEAIGGKFVPRSILCDLEPGTMDAVRCGPVGQLFRPDNYVFGSTGAGNNWAKGHYTDGAELIDNVLEVVRKEAELCECMQGFQVVHALGGGTGAGMGTLLINKVREEFPDRVVCSFSTVPSPKVSDVVVEPYNATLSIHQLVENCDETFCIDNEALYDICNRTLKLSTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLATNMVPFPRLHFFMPGFSPLTSRHSMPYRSNTVQDLTHQMFDNKNMMAACDPRHGKYLTVAAMFRGRMSMKEVDEQMLNVQNRNSAYFVEWIPNNVKTAVCDIPPRGLKMSVTFIGNNTAIQEIFKRVGEQFVAMFRRRAFLHWYTGEGMDEMEFSE 410          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB2A (tubulin beta-2A chain [Source:NCBI gene;Acc:103041736])

HSP 1 Score: 701.819 bits (1810), Expect = 0.000e+0
Identity = 331/411 (80.54%), Postives = 378/411 (91.97%), Query Frame = 3
Query:  156 HMREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            +MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y+G+S+LQLERINVYYNEATG + VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKESE C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DETF+IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  SV +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+ML+VQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+F+RI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:   77 NMREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGSYQGESELQLERINVYYNEATGSKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 487          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: zgc:55461 (tubulin beta-4B chain [Source:NCBI gene;Acc:103024068])

HSP 1 Score: 699.123 bits (1803), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMA TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB1 (tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HGNC:16257])

HSP 1 Score: 582.793 bits (1501), Expect = 0.000e+0
Identity = 283/410 (69.02%), Postives = 333/410 (81.22%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q+GQCGNQ+G+K  E+                             +  GG+ VPR +L DLEP TMDSV+    G LFRPDNF +G++GAGNNWAKGHYT+GAEL++ V++ VR E+E+C+CLQGFQ VHSLGGGTGSGMGTL+++KIREE+PDRI+ +FS  PSPKVSD VVEPYNATL+IHQL+E  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVS TMSGVTT LRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLT+R SQ YR  +V +LTQQMFD +N+M ACDPR G+YLTVA +FRGR+S KEVDE+ML +Q KNS YFV+WIP NVK AVCDIPPRG+KMA TFIGNNTAIQEIF+R+ EQ+A M+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQIGQCGNQIGSKVTEI--------------------------RLIDTCGGKYVPRALLVDLEPGTMDSVRGSRIGQLFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRNEAESCDCLQGFQFVHSLGGGTGSGMGTLIINKIREEYPDRIMNSFSIMPSPKVSDTVVEPYNATLSIHQLIENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDARNMMTACDPRRGRYLTVAGIFRGRMSTKEVDEQMLAIQQKNSNYFVDWIPHNVKVAVCDIPPRGLKMASTFIGNNTAIQEIFRRVGEQFAMMFRRKAFLHWYTGEGMDEMEFTE 384          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000019550.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000026.1:1405913:1407004:-1 gene:ENSAMXG00000018987.2 transcript:ENSAMXT00000019550.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 546.969 bits (1408), Expect = 0.000e+0
Identity = 264/322 (81.99%), Postives = 301/322 (93.48%), Query Frame = 3
Query:  423 NFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            + + G +GAGNNWAKGHYT+GAEL+DSVL++VRKESE+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQ+FD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMAVTFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    8 HLSAGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 329          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000019223.2 (tubulin alpha-1 chain [Source:NCBI gene;Acc:103031418])

HSP 1 Score: 340.502 bits (872), Expect = 7.987e-113
Identity = 175/421 (41.57%), Postives = 252/421 (59.86%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLER---INVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPR--------GMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MRE + I VGQ G Q G   WE+   EHG+  DG  +  + L   R    N ++N  + GR VPR ++ DLEP+ +D V+ G Y  LF P+    G   A NN+A+GHYT G E+ID VLE VRK ++ C  LQGF + HS GGGTGSG  +LL+ ++  ++  +    F+ YP+P+VS  VVEPYN+ L  H  +E +D  F +DNEA+YDIC R L +  P+Y +LN L+   +S +T  LRF G LN DL +  TNL+P+PR+HF +  ++P+ S     +   SV ++T   F+  N M  CDPRHGKY+    ++RG +  K+V+  + N++ K S  FV+W P+  K  +   PP          ++ AV  + N TAI E + R+  ++  MY ++AF+HWY GEGM+E EF+E
Sbjct:    1 MRECISIHVGQAGIQTGNACWELFCLEHGVGPDGVMQDVAALSNSRQDPFNTFFNTGSSGRHVPRAVMVDLEPTVVDEVRSGIYRQLFHPEQLISGKEDAANNYARGHYTVGKEIIDVVLERVRKLTDQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPSYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISTEKAYHEQLSVAEITSACFEPSNQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIANIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 421          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubb2 (tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GENE-050522-384])

HSP 1 Score: 604.749 bits (1558), Expect = 0.000e+0
Identity = 292/375 (77.87%), Postives = 340/375 (90.67%), Query Frame = 3
Query:  213 KFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTF-STYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRK 1334
            +FWEVISDEHG+   G Y GDSDLQLERINVYYNEA+GG+ VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+  +++ T  ++ PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRK
Sbjct:    1 QFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYIIQVLYTVVASGPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRK 375          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 284.263 bits (726), Expect = 5.335e-93
Identity = 144/349 (41.26%), Postives = 211/349 (60.46%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQL--ERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPS 1199
            MRE + I VGQ G Q+G   WE+   EHG+  DG    D  +    +  N +++E   G+ VPR +  DLEP+ +D V+ G Y  LF P+    G   A NN+A+GHYT G E++D VL+ +RK ++ C  LQGF + HS GGGTGSG  +LL+ ++  ++  +    F+ YP+P+VS  VVEPYN+ L  H  +E +D  F +DNEA+YDIC R L +  PTY +LN L+   +S +T  LRF G LN DL +  TNL+P+PR+HF +  +AP+ S     +   SV ++T   F+  N M  CDPRHGKY+    ++RG +  K+V+  +  ++ K +  FV+W P+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPT 349          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubg1 (tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-836])

HSP 1 Score: 243.047 bits (619), Expect = 7.618e-76
Identity = 131/419 (31.26%), Postives = 233/419 (55.61%), Query Frame = 3
Query:  162 REIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGH--TGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSP-KVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHS-QPYRTYSVHDLTQQMFDCKNLMAACDPRHGK---YLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPP---RGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            REI+ +Q+GQCGNQ+G +FW+ +  EHG+S DG  +  +    +R +V++ +A     +PR +L DLEP  ++S+    Y +L+ P+N        GAGNNWA GH + G ++ + + +++ +E++  + L+   +   L     + +      K    FP +++ T+S +P+  ++SDVVV+PYN+ L + +L + AD    +DN AL  I    L + NP++  +N LVS  MS  TT LR+PG +N DL  L  +LIP PRLHF + G+ PLT+  S    R  +V D+ +++   KN+M +          Y+ +  + +G +   +V + +  ++ +  A F+ W P++++ A+    P      +++   + N+T+I  +F R  +QY  + +R+AFL  +  E M + + +E
Sbjct:    3 REIITLQLGQCGNQIGFEFWKQLCAEHGISPDGILEDFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVINSIVNSSYANLYNPENIYLSEHGGGAGNNWASGH-SQGEKIHEEIFDIIDREADGSDSLEKCPLTRRLSATNWNELC-----KYFCRFPKKLIQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLTSAHRVSGLMMANHTSISSLFDRTCKQYDKLRKREAFLDQFRKEDMFKEDLSE 415          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001139.1 (pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 gene:ENSPMAG00000001018.1 transcript:ENSPMAT00000001139.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 231.106 bits (588), Expect = 3.200e-72
Identity = 130/353 (36.83%), Postives = 195/353 (55.24%), Query Frame = 3
Query:  162 REIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQL---ERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCG----PYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPS 1199
            R+ + I +GQ G QMG   WE+   EHG+  DG      D+        N +++E   G+ VPR I  DLEPS +    CG      G   R      G     +   +G    G ++   VL   R +SE C  LQGF + HS GGGTGSG  +LL+ ++  ++  +    F  YP+P+VS  VVEPYNA L  H  +E +D +F +DNEA+YDIC + L +  P+Y +LN L++  +S +T  LRF G LN DL +  TNL+P+PR+HF +  +AP+ S     +   SV ++T   F+  N M  CDPR+GKY+    ++RG +  K+V+  +  ++++ +  FV+W P+
Sbjct:    3 RDCISIHIGQAGVQMGNACWELYCLEHGIKPDGHLATHPDVTSVVDSSFNTFFSETGSGKHVPRAIFIDLEPSVIGE-PCGLRTLTVGGXLRMSMMLAGREVRSSRIWRGQKGGGRKICSRVLRRNRPQSERCTGLQGFLLFHSFGGGTGSGFTSLLMERLSADYGKKSKLEFCIYPAPQVSTAVVEPYNAILTTHTTLEHSDCSFMVDNEAIYDICRKKLDIDFPSYFNLNRLIAQMVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLTTYAPVISAEKAYHEQLSVAEITNACFEPSNQMVKCDPRNGKYMACCLLYRGDIVPKDVNTAIAIIKSRRAVRFVDWCPT 354          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009623.1 (pep scaffold:Pmarinus_7.0:GL485233:4234:10583:1 gene:ENSPMAG00000008696.1 transcript:ENSPMAT00000009623.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 221.09 bits (562), Expect = 3.999e-71
Identity = 114/159 (71.70%), Postives = 140/159 (88.05%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKG-DSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREE 632
            MREIV++Q GQCGNQ+G+KFWEVIS+EHG+   G Y+G ++ LQ+ERINVYY+EAT G+ VPR I+ DLEP TMD+V+ GP G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE
Sbjct:    1 MREIVHVQAGQCGNQVGSKFWEVISEEHGIDRTGSYRGYNARLQMERINVYYSEATSGKFVPRAIMVDLEPGTMDAVRSGPLGHMFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREE 159          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB2 (Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria [Source:SGD;Acc:S000001857])

HSP 1 Score: 592.038 bits (1525), Expect = 0.000e+0
Identity = 278/410 (67.80%), Postives = 349/410 (85.12%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREI++I  GQCGNQ+GA FWE I  EHGL  +G Y G  D+Q ER+NVY+NEA+ G+ VPR+I  DLEP T+D+V+    G+LFRPDN+ +G + AGN WAKGHYT+GAEL+DSV++++R+E+E C+ LQGFQ+ HSLGGGTGSGMGTLL+SKIREEFPDR++ TFS  PSPK SD VVEPYNATL++HQLVE +DETF IDNEALYDIC RTLKL+ P+YGDLN+LVS+ MSGVTT LR+PGQLN+DLRKL  NL+PFPRLHFF+ G+APLT+  SQ +R+ +V +LTQQMFD KN+MAA DPR+G+YLTVAA FRG++SVKEV++EM  VQ+KNS YFVEWIP+NV+TAVC + P+G+ MA TFI N+T+IQE+FKR+ +Q++AM++RKAFLHWYT EGMDE+EF+E
Sbjct:    1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQKERLNVYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPELTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAVCSVAPQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEFSE 410          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB1 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication [Source:SGD;Acc:S000004550])

HSP 1 Score: 336.65 bits (862), Expect = 3.243e-112
Identity = 178/421 (42.28%), Postives = 260/421 (61.76%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYK-GDSDLQ--LERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRG--------MKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MRE++ I VGQ G Q+G   WE+ S EHG+  DG  + G S  +   E  + +++E   G+ VPR I  DLEP+ +D V+ GPY  LF P+    G   A NN+A+GHYT G E++  VL+ +RK ++ C+ LQGF   HSLGGGTGSG+G+LLL ++  E+  +    F+ YP+P+VS  VVEPYN  L  H  +E AD TF +DNEA+YD+C R L +  P++ +LN+L++  +S VT  LRF G LN DL +  TNL+P+PR+HF +  ++P+ S+    + + SV ++T   F+  N M  CDPR GKY+    ++RG +  ++V   +  V+NK +   V+W P+  K  +C  PP          +  AV  + N T+I E +KRI  ++  MY ++AF+HWY GEGM+E EFTE
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKPDGHLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRNGPYKDLFHPEQLISGKEDAANNYARGHYTVGREILGDVLDRIRKLADQCDGLQGFLFTHSLGGGTGSGLGSLLLEELSAEYGKKSKLEFAVYPAPQVSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCKRNLDIPRPSFANLNNLIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYSPVLSKSKAFHESNSVSEITNACFEPGNQMVKCDPRDGKYMATCLLYRGDVVTRDVQRAVEQVKNKKTVQLVDWCPTGFKIGICYEPPTATPNSQLATVDRAVCMLSNTTSIAEAWKRIDRKFDLMYAKRAFVHWYVGEGMEEGEFTE 421          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB3 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication [Source:SGD;Acc:S000004593])

HSP 1 Score: 335.109 bits (858), Expect = 1.298e-111
Identity = 178/421 (42.28%), Postives = 258/421 (61.28%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYK-GDSDLQ--LERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPR--------GMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MRE++ I VGQ G Q+G   WE+ S EHG+  DG  + G S  +   E  + +++E   G+ VPR I  DLEP+ +D V+ G +  LF P+    G   A NN+A+GHYT G E++D V E +RK ++ C+ LQGF   HSLGGGTGSG+G+LLL  +  E+  +    F+ YP+P++S  VVEPYN  L  H  +E AD TF +DNEA+YDIC R L +S P++ +LN L++  +S VT  LRF G LN DL +  TNL+P+PR+HF +  +AP+ S+    + + SV ++T   F+  N M  CDP  GKY+    ++RG +  ++V   +  V+NK +   V+W P+  K  +C  PP          +  AV  + N TAI + +KRI +++  MY ++AF+HWY GEGM+E EFTE
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKEDGHLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAANNYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLENLSYEYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNLGISRPSFSNLNGLIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPILSKKRATHESNSVSEITNACFEPGNQMVKCDPTKGKYMANCLLYRGDVVTRDVQRAVEQVKNKKTVQMVDWCPTGFKIGICYEPPSVIPSSELANVDRAVCMLSNTTAIADAWKRIDQKFDLMYAKRAFVHWYVGEGMEEGEFTE 421          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB4 (Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004202])

HSP 1 Score: 174.096 bits (440), Expect = 1.549e-49
Identity = 118/410 (28.78%), Postives = 205/410 (50.00%), Query Frame = 3
Query:  165 EIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLER---INVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDN--FAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQ---PYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNV------KTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWY 1352
            EI+ +Q GQCGN +G   W  ++ EH +  DG  +   D   ER      ++ E +  +  PR I+ D EPS +  V+   +   F P N   A     AGN+WA G Y  G    D +L  + KE ++ +  +GFQ++HS+ GGTGSG+G+ LL  + + +P +I+ T+S +P+ + S+VVV+ YN  LA+ +L+E +D T   DN +L +I  +  +  N      N L+S  +S VT  +RFP  + + +  + + LIP P LHF  P F P TS +      ++ +S +D+   + D  N + +    +  Y  V     G +  +++   M  +Q +    F  W  S +      ++    + P   +++   + N + +  +F+     +  ++ + AFL+ Y
Sbjct:    4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQL-PDSSTERDDDTKPFFRENSRNKFTPRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVFPA-RSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNY 407          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO39945 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8I3])

HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0
Identity = 333/410 (81.22%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+ Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAAMFRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHCQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO35958 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJX9])

HSP 1 Score: 697.582 bits (1799), Expect = 0.000e+0
Identity = 331/410 (80.73%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV +Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR++L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+C+QGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAAMFRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVSLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRSVLIDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELMDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSIVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO42890 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S027])

HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0
Identity = 321/410 (78.29%), Postives = 369/410 (90.00%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFW    +EHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+C+QGFQ+ HSLGGGTGSGM TLL+ KIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRL FF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDP HG+YLTVAAMFRGR+S+KE+DE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFW----NEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMVTLLILKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLPFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPLHGRYLTVAAMFRGRMSMKELDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 406          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO49518 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGG3])

HSP 1 Score: 595.119 bits (1533), Expect = 0.000e+0
Identity = 278/412 (67.48%), Postives = 343/412 (83.25%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYK--GDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREI+ IQVGQCGNQ+G +FW  I++EHG+   G      D+  + +RI+VY++E  GGR VPR+IL DLEP TMD+V+ GP G++FRPDNF    +GA NNWAKGHY++GAELID++ +++RKE+E C+C+QG Q++ +LGGGTGSG+GTL+LSKIREE+PDRI+  FS  PSPKVS+ VVEPYNATL++HQLVE+ DE F  DNEALYDIC RTLKL+ P Y DLN LVS  M G+T  LRFPGQLN DLRKL  N++PFPRLHFF+PGFAPL SR +Q Y+  SV +LTQQMFD +N+M ACDPRHG+YLTVAA+FRG++S++EVDE+MLN QNKNS +FVEWIP+NVKT VCDI P+G+KMA TF+GNNTAIQE+FKR+ EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIINIQVGQCGNQIGTEFWRDIAEEHGIDPTGAKSLIEDTHGRFDRIDVYFHEGNGGRYVPRSILVDLEPGTMDAVRGGPLGNMFRPDNFVNSQSGAANNWAKGHYSEGAELIDNIFDVLRKEAENCDCMQGVQLIQALGGGTGSGLGTLILSKIREEYPDRILSAFSVVPSPKVSETVVEPYNATLSVHQLVEVVDEDFMFDNEALYDICCRTLKLAKPNYADLNQLVSMAMCGLTASLRFPGQLNGDLRKLAVNMVPFPRLHFFMPGFAPLASRANQQYQNVSVEELTQQMFDARNMMTACDPRHGRYLTVAAIFRGKMSMREVDEQMLNTQNKNSPFFVEWIPNNVKTTVCDIAPKGLKMAATFVGNNTAIQELFKRVGEQFTAMFRRKAFLHWYTGEGMDEMEFTE 412          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO36734 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHY4])

HSP 1 Score: 547.354 bits (1409), Expect = 0.000e+0
Identity = 263/318 (82.70%), Postives = 298/318 (93.71%), Query Frame = 3
Query:  435 GHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAAMFRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    3 GQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 320          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb5 (tubulin beta chain [Source:NCBI gene;Acc:101157969])

HSP 1 Score: 697.582 bits (1799), Expect = 0.000e+0
Identity = 333/410 (81.22%), Postives = 378/410 (92.20%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQL+RI+VYYNEATGG+ VPR IL DLEP TMDSV+ GP+G +FRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKESE+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQ+FD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KMAVTFIGN+TAIQE+FKRI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb2 (tubulin beta-2B chain [Source:NCBI gene;Acc:101162245])

HSP 1 Score: 697.197 bits (1798), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 376/410 (91.71%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQ GQCGNQ+GAKFWEVISDEHG+   G Y+GDSDLQLERINVYYNEA+G + VPR IL DLEP TMDSV+ GP+G LFRPDNF +G +GAGNNWAKGHYT+GAEL+D+VL++VRKESE C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DETF IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  SV +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S++EVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+F+RI+EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYQGDSDLQLERINVYYNEASGSKYVPRAILVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000045809.1 (tubulin beta-6 chain [Source:NCBI gene;Acc:101166121])

HSP 1 Score: 647.121 bits (1668), Expect = 0.000e+0
Identity = 303/410 (73.90%), Postives = 361/410 (88.05%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q+GQCGNQ+G+KFWEVIS+EHG++  G Y+GDS LQLERINVY++EA GG+ VPR +L DLEP TMDS++    G+LFRPDNF +G++GAGNNWAKGHYT+GAEL++ V++ VR ESE C+CLQGFQ+VHSLGGGTGSGMGTL+++KI+EEFPDRI+ TFS  PSPKVSD VVEPYNATL++HQL+E AD TF IDNEALYDICFRTLKL+ PTYGDLNHLV  TMSGVTT LRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLT+R SQ YR  +V +LTQQMFD +N+M ACDPR G+YLTVA +FRG++S KEVDE+ML +Q KNS YFV+WIP NVK AVCDIPP+G++MA TFIGNNTAIQEIF+R+ EQ++ M+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHVQIGQCGNQIGSKFWEVISEEHGINATGIYEGDSTLQLERINVYFSEAYGGKYVPRALLVDLEPGTMDSIRGSRIGALFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRHESEGCDCLQGFQLVHSLGGGTGSGMGTLIINKIKEEFPDRIMNTFSIMPSPKVSDTVVEPYNATLSVHQLLENADATFCIDNEALYDICFRTLKLTTPTYGDLNHLVCMTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDARNMMTACDPRRGRYLTVAGIFRGKMSTKEVDEQMLAIQQKNSTYFVDWIPHNVKVAVCDIPPKGLRMASTFIGNNTAIQEIFRRVGEQFSLMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000033163.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 339.732 bits (870), Expect = 1.550e-112
Identity = 176/420 (41.90%), Postives = 251/420 (59.76%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQL--ERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPR--------GMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MRE + I VGQ G Q+G   WE+   EHG+  DG    D  +    +  N +++E   G+ VPR +  DLEP+ +D V+ G Y  LF P+    G   A NN+A+GHYT G E+ID VL+ +RK ++ C  LQGF V HS GGGTGSG  +LL+ ++  ++  +    FS YP+P+VS  VVEPYNA L  H  +E +D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +  TNL+P+PR+HF +  +AP+ S     +   SV ++T   F+  N M  CDPRHGKY+    ++RG +  K+V+  +  ++ K S  FV+W P+  K  +   PP          ++ AV  + N TAI E + R+  ++  MY ++AF+HWY GEGM+E EF+E
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000020578.2 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 339.732 bits (870), Expect = 1.550e-112
Identity = 176/420 (41.90%), Postives = 251/420 (59.76%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQL--ERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPR--------GMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MRE + I VGQ G Q+G   WE+   EHG+  DG    D  +    +  N +++E   G+ VPR +  DLEP+ +D V+ G Y  LF P+    G   A NN+A+GHYT G E+ID VL+ +RK ++ C  LQGF V HS GGGTGSG  +LL+ ++  ++  +    FS YP+P+VS  VVEPYNA L  H  +E +D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +  TNL+P+PR+HF +  +AP+ S     +   SV ++T   F+  N M  CDPRHGKY+    ++RG +  K+V+  +  ++ K S  FV+W P+  K  +   PP          ++ AV  + N TAI E + R+  ++  MY ++AF+HWY GEGM+E EF+E
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000062548.1 (SMESG000062548.1)

HSP 1 Score: 781.556 bits (2017), Expect = 0.000e+0
Identity = 378/410 (92.20%), Postives = 378/410 (92.20%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELV                                SKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELV-------------------------------FSKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 379          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000048308.1 (SMESG000048308.1)

HSP 1 Score: 743.421 bits (1918), Expect = 0.000e+0
Identity = 356/410 (86.83%), Postives = 390/410 (95.12%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV+IQVGQCGNQMG KFWEVISDEHG+SVDGFYKGDSDLQLERI VYYNEATGG+ VPR ILTDLEP TMDS++CG  G+LFRPDNF +G TGAGNNWAKGHYTDGAELID+V+E+VRKESE CECLQGFQ+VH+LGGGTG+GMGTLL+SKIREEFPDRIVC+FS  PSPKVSDVVVEPYNATL+IHQLVE  DET+ IDNEALYDIC RTLKL++PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL TN+IPFPRLHFF+PGF+PLTSRHSQPYR YSV DLTQQMFDCKN+MAACDPRHGKYLTVAAMFRGR+S+KEVDE+MLNVQNKNSAYFVEWIP+NVKTAVCDIPPRG+KM+VTFIGNNTAIQEIFKR+ EQ++AM+RR+AFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHIQVGQCGNQMGGKFWEVISDEHGISVDGFYKGDSDLQLERICVYYNEATGGKFVPRAILTDLEPGTMDSLRCGVVGNLFRPDNFIFGSTGAGNNWAKGHYTDGAELIDNVIEVVRKESENCECLQGFQIVHALGGGTGAGMGTLLISKIREEFPDRIVCSFSCVPSPKVSDVVVEPYNATLSIHQLVENCDETYCIDNEALYDICNRTLKLTSPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLATNMIPFPRLHFFMPGFSPLTSRHSQPYRCYSVQDLTQQMFDCKNMMAACDPRHGKYLTVAAMFRGRMSMKEVDEQMLNVQNKNSAYFVEWIPNNVKTAVCDIPPRGLKMSVTFIGNNTAIQEIFKRVCEQFSAMFRRRAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000074579.1 (SMESG000074579.1)

HSP 1 Score: 701.819 bits (1810), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR IL DLEP TMDSV+ GP+G LFRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAAMFRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+N KTAVCDIPPRG+KM+VTFIGN+T+IQE+FKR++EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNAKTAVCDIPPRGLKMSVTFIGNSTSIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000074423.1 (SMESG000074423.1)

HSP 1 Score: 701.819 bits (1810), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR IL DLEP TMDSV+ GP+G LFRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DET+ IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAAMFRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+N KTAVCDIPPRG+KM+VTFIGN+T+IQE+FKR++EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNAKTAVCDIPPRGLKMSVTFIGNSTSIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000035755.1 (SMESG000035755.1)

HSP 1 Score: 701.049 bits (1808), Expect = 0.000e+0
Identity = 332/410 (80.98%), Postives = 377/410 (91.95%), Query Frame = 3
Query:  159 MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVYYNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNNWAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHXXXXXXXXXXXXXXXXKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETFTIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLMAACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVKTAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTGEGMDEMEFTE 1388
            MREIV++Q GQCGNQ+GAKFWEVISDEHG+   G Y GDSDLQLERINVYYNEATGG+ VPR +L DLEP TMDSV+ GP+G LFRPDNF +G +GAGNNWAKGHYT+GAEL+DSVL++VRKE+E+C+CLQGFQ+ HSLGGGTGSGMGTLL+SKIREE+PDRI+ TFS  PSPKVSD VVEPYNATL++HQLVE  DETF IDNEALYDICFRTLKL+ PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKL  N++PFPRLHFF+PGFAPLTSR SQ YR  +V +LTQQMFD KN+MAACDPRHG+YLTVAA+FRGR+S+KEVDE+MLNVQNKNS+YFVEWIP+NVKTAVCDIPPRG+KM+ TFIGN+TAIQE+FKR++EQ+ AM+RRKAFLHWYTGEGMDEMEFTE
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410          
The following BLAST results are available for this feature:
BLAST of Tubulin beta chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBB2A0.000e+080.98tubulin beta 2A class IIa [Source:HGNC Symbol;Acc:... [more]
TUBB2B0.000e+081.22tubulin beta 2B class IIb [Source:HGNC Symbol;Acc:... [more]
TUBB4B0.000e+080.73tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:... [more]
TUBB0.000e+080.98tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB0.000e+080.98tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Tubulin beta chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tbb-40.000e+080.98Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot... [more]
ben-10.000e+079.51Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q... [more]
tbb-10.000e+078.05Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:O... [more]
tbb-10.000e+078.05Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:O... [more]
tbb-20.000e+077.80Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot... [more]
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BLAST of Tubulin beta chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
betaTub56D0.000e+079.76gene:FBgn0284243 transcript:FBtr0086536[more]
betaTub85D0.000e+079.51gene:FBgn0003889 transcript:FBtr0082046[more]
betaTub60D0.000e+078.31gene:FBgn0003888 transcript:FBtr0343443[more]
betaTub60D0.000e+078.13gene:FBgn0003888 transcript:FBtr0072270[more]
betaTub97EF0.000e+077.56gene:FBgn0003890 transcript:FBtr0300611[more]
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BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:554610.000e+080.98zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+080.98zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+080.98zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+080.98zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:1534260.000e+080.98zgc:153426 [Source:ZFIN;Acc:ZDB-GENE-060929-220][more]
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BLAST of Tubulin beta chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
znf3300.000e+080.98zinc finger protein 330 [Source:Xenbase;Acc:XB-GEN... [more]
reps20.000e+080.73RALBP1 associated Eps domain containing 2 [Source:... [more]
ubxn110.000e+080.73UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-... [more]
tubb0.000e+080.98tubulin beta class I [Source:NCBI gene;Acc:448742][more]
tubb30.000e+078.54tubulin beta 3 class III [Source:NCBI gene;Acc:395... [more]
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BLAST of Tubulin beta chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tubb2b0.000e+081.22tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:... [more]
Tubb2a0.000e+080.98tubulin, beta 2A class IIA [Source:MGI Symbol;Acc:... [more]
Tubb4b0.000e+080.73tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:... [more]
Tubb50.000e+080.98tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI... [more]
Tubb4a0.000e+080.49tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:... [more]
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BLAST of Tubulin beta chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9NFZ6|TBB2_ECHMU0.000e+080.49Tubulin beta-2 chain OS=Echinococcus multiloculari... [more]
sp|O44388|TBB_TRITR0.000e+081.46Tubulin beta chain OS=Trichuris trichiura OX=36087... [more]
sp|P30883|TBB4_XENLA0.000e+080.73Tubulin beta-4 chain OS=Xenopus laevis OX=8355 GN=... [more]
sp|P32882|TBB2_CHICK0.000e+081.22Tubulin beta-2 chain OS=Gallus gallus OX=9031 PE=1... [more]
sp|Q3KRE8|TBB2B_RAT0.000e+081.22Tubulin beta-2B chain OS=Rattus norvegicus OX=1011... [more]
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BLAST of Tubulin beta chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A419PIP40.000e+083.41Tubulin beta chain OS=Clonorchis sinensis OX=79923... [more]
A0A0V0J3W30.000e+084.39Tubulin beta chain OS=Schistocephalus solidus OX=7... [more]
A0A4S2LW180.000e+083.41Tubulin beta chain OS=Opisthorchis felineus OX=147... [more]
I6TL060.000e+083.90Tubulin beta chain OS=Spirometra erinaceieuropaei ... [more]
A0A074ZDG40.000e+083.17Tubulin beta chain OS=Opisthorchis viverrini OX=61... [more]
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BLAST of Tubulin beta chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBB2A0.000e+080.54tubulin beta-2A chain [Source:NCBI gene;Acc:103041... [more]
zgc:554610.000e+080.98tubulin beta-4B chain [Source:NCBI gene;Acc:103024... [more]
TUBB10.000e+069.02tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HG... [more]
ENSAMXT00000019550.20.000e+081.99pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000019223.27.987e-11341.57tubulin alpha-1 chain [Source:NCBI gene;Acc:103031... [more]
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BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb20.000e+077.87tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GE... [more]
TUBA3C5.335e-9341.26tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
tubg17.618e-7631.26tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-... [more]
ENSPMAT00000001139.13.200e-7236.83pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 g... [more]
ENSPMAT00000009623.13.999e-7171.70pep scaffold:Pmarinus_7.0:GL485233:4234:10583:1 ge... [more]
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BLAST of Tubulin beta chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
TUB20.000e+067.80Beta-tubulin; associates with alpha-tubulin (Tub1p... [more]
TUB13.243e-11242.28Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB31.298e-11142.28Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB41.549e-4928.78Gamma-tubulin; involved in nucleating microtubules... [more]
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BLAST of Tubulin beta chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO399450.000e+081.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO359580.000e+080.73Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO428900.000e+078.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495180.000e+067.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO367340.000e+082.70Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tubulin beta chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb50.000e+081.22tubulin beta chain [Source:NCBI gene;Acc:101157969... [more]
tubb20.000e+080.98tubulin beta-2B chain [Source:NCBI gene;Acc:101162... [more]
ENSORLT00000045809.10.000e+073.90tubulin beta-6 chain [Source:NCBI gene;Acc:1011661... [more]
ENSORLT00000033163.11.550e-11241.90tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
ENSORLT00000020578.21.550e-11241.90tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
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BLAST of Tubulin beta chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000062548.10.000e+092.20SMESG000062548.1[more]
SMESG000048308.10.000e+086.83SMESG000048308.1[more]
SMESG000074579.10.000e+080.98SMESG000074579.1[more]
SMESG000074423.10.000e+080.98SMESG000074423.1[more]
SMESG000035755.10.000e+080.98SMESG000035755.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035933 ID=SMED30035933|Name=Tubulin beta chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1388bp
TTGAATGTATAATATTTCACGTATGTGTAATAAAAAGGCTCCTTATACTC
GTTGCTACGATATTTATTTAAATCAATTTTATTATTTAAAAGTTTAAAAA
TATCAATTAGAAAAAATAAATATCGTAGCAACGAGAATAAGGAGCCTAAT
TATTACACATGCGTGAAATAGTATACATTCAAGTAGGACAATGTGGAAAT
CAGATGGGAGCTAAGTTTTGGGAAGTAATTTCTGATGAACATGGTTTAAG
TGTTGACGGATTTTACAAAGGTGACAGTGATTTACAGTTAGAAAGAATCA
ATGTTTACTACAACGAAGCCACTGGTGGTCGTTTGGTACCTAGAACAATA
TTGACTGATTTAGAACCAAGTACAATGGACTCAGTAAAATGCGGACCATA
TGGAAGCTTATTTCGCCCAGATAACTTTGCCTATGGGCATACAGGAGCCG
GTAATAATTGGGCTAAAGGCCATTATACAGATGGTGCTGAACTAATTGAT
TCTGTTCTTGAATTGGTGCGAAAAGAATCTGAAGCATGTGAATGCTTACA
AGGTTTTCAGGTAGTTCATTCACTTGGTGGAGGTACTGGGTCTGGAATGG
GTACTTTGTTATTAAGTAAAATACGTGAAGAATTTCCGGATCGAATTGTT
TGCACATTTTCCACTTATCCTTCTCCAAAAGTATCAGATGTTGTAGTTGA
GCCATATAATGCCACTTTGGCAATTCATCAGTTAGTTGAGATGGCAGATG
AAACTTTCACAATAGACAACGAAGCTCTTTATGATATTTGTTTTAGAACA
TTAAAACTTAGTAATCCAACTTATGGTGATTTAAATCATTTAGTTAGTGC
GACAATGTCTGGAGTCACAACATGTTTAAGATTTCCTGGTCAACTAAATG
CAGATTTGAGAAAATTAGGAACAAATTTAATACCATTTCCTAGATTGCAT
TTTTTTGTACCTGGATTTGCACCACTAACAAGTAGACATTCGCAACCCTA
TAGAACTTATTCTGTTCATGATCTTACTCAGCAAATGTTTGATTGTAAGA
ATTTAATGGCAGCTTGTGATCCAAGACATGGAAAATATTTAACAGTAGCA
GCAATGTTTAGAGGAAGATTATCCGTTAAAGAAGTTGATGAAGAAATGCT
AAATGTTCAAAATAAAAATAGTGCATATTTCGTCGAATGGATACCAAGTA
ATGTAAAAACTGCAGTTTGTGATATTCCTCCAAGAGGAATGAAGATGGCA
GTGACCTTTATTGGAAACAACACAGCAATACAAGAGATTTTTAAGAGAAT
TGCGGAACAATATGCAGCAATGTATAGACGAAAAGCTTTTCTACATTGGT
ATACTGGAGAAGGAATGGATGAAATGGAGTTTACTGAA
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protein sequence of SMED30035933-orf-1

>SMED30035933-orf-1 ID=SMED30035933-orf-1|Name=SMED30035933-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=410bp
MREIVYIQVGQCGNQMGAKFWEVISDEHGLSVDGFYKGDSDLQLERINVY
YNEATGGRLVPRTILTDLEPSTMDSVKCGPYGSLFRPDNFAYGHTGAGNN
WAKGHYTDGAELIDSVLELVRKESEACECLQGFQVVHSLGGGTGSGMGTL
LLSKIREEFPDRIVCTFSTYPSPKVSDVVVEPYNATLAIHQLVEMADETF
TIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADL
RKLGTNLIPFPRLHFFVPGFAPLTSRHSQPYRTYSVHDLTQQMFDCKNLM
AACDPRHGKYLTVAAMFRGRLSVKEVDEEMLNVQNKNSAYFVEWIPSNVK
TAVCDIPPRGMKMAVTFIGNNTAIQEIFKRIAEQYAAMYRRKAFLHWYTG
EGMDEMEFTE
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0002030early male germ cell
PLANA:0003304testis primordium
Vocabulary: INTERPRO
TermDefinition
IPR037103Tubulin/FtsZ_C_sf
IPR036525Tubulin/FtsZ_GTPase_sf
IPR013838Beta-tubulin_BS
IPR002453Beta_tubulin
IPR000217Tubulin
IPR003008Tubulin_FtsZ_GTPase
IPR018316Tubulin/FtsZ_2-layer-sand-dom
IPR023123Tubulin_C
IPR017975Tubulin_CS
IPR008280Tub_FtsZ_C
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
GO:0007010cytoskeleton organization
Vocabulary: molecular function
TermDefinition
GO:0005525GTP binding
GO:0005200structural constituent of cytoskeleton
GO:0003924GTPase activity
GO:0000166nucleotide binding
Vocabulary: cellular component
TermDefinition
GO:0005874microtubule
GO:0005737cytoplasm
GO:0005856cytoskeleton
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002453Beta tubulinPRINTSPR01163BETATUBULINcoord: 329..343
score: 78.02
coord: 230..239
score: 83.43
coord: 377..388
score: 56.29
coord: 212..224
score: 79.21
coord: 347..370
score: 73.17
coord: 88..99
score: 67.0
coord: 311..329
score: 68.57
coord: 41..58
score: 81.05
coord: 245..258
score: 73.1
coord: 108..126
score: 66.59
coord: 152..164
score: 72.53
coord: 265..281
score: 71.06
IPR000217TubulinPRINTSPR01161TUBULINcoord: 93..104
score: 69.02
coord: 151..172
score: 48.52
coord: 370..398
score: 58.96
coord: 51..70
score: 58.02
coord: 10..30
score: 67.1
coord: 132..150
score: 78.03
coord: 190..210
score: 65.31
coord: 106..130
score: 55.49
coord: 176..189
score: 68.58
IPR000217TubulinPANTHERPTHR11588TUBULINcoord: 2..410
IPR003008Tubulin/FtsZ, GTPase domainSMARTSM00864Tubulin_4coord: 47..244
e-value: 2.6E-65
score: 232.9
IPR003008Tubulin/FtsZ, GTPase domainPFAMPF00091Tubulincoord: 3..211
e-value: 6.5E-66
score: 222.3
IPR018316Tubulin/FtsZ, 2-layer sandwich domainSMARTSM00865Tubulin_C_4coord: 246..383
e-value: 2.6E-40
score: 149.9
IPR018316Tubulin/FtsZ, 2-layer sandwich domainPFAMPF03953Tubulin_Ccoord: 261..382
e-value: 1.8E-41
score: 141.2
IPR023123Tubulin, C-terminalGENE3DG3DSA:1.10.287.600coord: 374..410
e-value: 2.3E-19
score: 70.9
IPR037103Tubulin/FtsZ, C-terminal domain superfamilyGENE3DG3DSA:3.30.1330.20coord: 264..373
e-value: 6.1E-53
score: 179.8
IPR036525Tubulin/FtsZ, GTPase domain superfamilyGENE3DG3DSA:3.40.50.1440coord: 1..263
e-value: 1.0E-118
score: 397.6
IPR036525Tubulin/FtsZ, GTPase domain superfamilySUPERFAMILYSSF52490Tubulin nucleotide-binding domain-likecoord: 1..243
NoneNo IPR availablePANTHERPTHR11588:SF340TUBULIN BETA CHAINcoord: 2..410
NoneNo IPR availableCDDcd02187beta_tubulincoord: 2..410
e-value: 0.0
score: 777.514
IPR017975Tubulin, conserved sitePROSITEPS00227TUBULINcoord: 140..146
IPR013838Beta tubulin, autoregulation binding sitePROSITEPS00228TUBULIN_B_AUTOREGcoord: 1..4
IPR008280Tubulin/FtsZ, C-terminalSUPERFAMILYSSF55307Tubulin C-terminal domain-likecoord: 244..410