Mitochondrial 28S ribosomal protein S21

Overview
NameMitochondrial 28S ribosomal protein S21
Smed IDSMED30035873
Length (bp)544
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Mitochondrial 28S ribosomal protein S21 (SMED30035873) t-SNE clustered cells

Violin plots show distribution of expression levels for Mitochondrial 28S ribosomal protein S21 (SMED30035873) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Mitochondrial 28S ribosomal protein S21 (SMED30035873) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Mitochondrial 28S ribosomal protein S21 (SMED30035873) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035873

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30035873 SmedASXL_019114SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
posterior sideSMED30035873 dd_Smed_v4_1993_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30035873 dd_Smed_v4_1993_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30035873 OX_Smed_1.0.17066ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Human
Match: MRPS21 (mitochondrial ribosomal protein S21 [Source:HGNC Symbol;Acc:HGNC:14046])

HSP 1 Score: 82.4185 bits (202), Expect = 1.591e-20
Identity = 36/82 (43.90%), Postives = 57/82 (69.51%), Query Frame = 2 
Query:   65 KNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPW 310
            K+ KF+ R+++V + +V++AY  L  +++ D + E +K + YYEKP   R R  YERC+RIY+ EM RKI+ +M+ +R DPW
Sbjct:    3 KHLKFIARTVMVQEGNVESAYRTLNRILTMDGLIEDIKHRRYYEKPCCRRQRESYERCRRIYNMEMARKINFLMRKNRADPW 84
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Human
Match: MRPS21 (mitochondrial ribosomal protein S21 [Source:HGNC Symbol;Acc:HGNC:14046])

HSP 1 Score: 82.4185 bits (202), Expect = 1.591e-20
Identity = 36/82 (43.90%), Postives = 57/82 (69.51%), Query Frame = 2 
Query:   65 KNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPW 310
            K+ KF+ R+++V + +V++AY  L  +++ D + E +K + YYEKP   R R  YERC+RIY+ EM RKI+ +M+ +R DPW
Sbjct:    3 KHLKFIARTVMVQEGNVESAYRTLNRILTMDGLIEDIKHRRYYEKPCCRRQRESYERCRRIYNMEMARKINFLMRKNRADPW 84
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Celegans
Match: mrps-21 (Mitochondrial Ribosomal Protein, Small [Source:UniProtKB/TrEMBL;Acc:Q9GYI2])

HSP 1 Score: 46.595 bits (109), Expect = 5.227e-7
Identity = 21/83 (25.30%), Postives = 49/83 (59.04%), Query Frame = 2 
Query:   56 IYFKNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKD 304
            I+  + +F  R+++V +NDV  A+  L  +M ++ + + ++  ++Y+KP + R  +  E    I++ +M RK+  +++ +R D
Sbjct:   23 IWNSHPRFATRTVMVQNNDVDGAFGLLNRLMDSEGMLKIIRRTQFYQKPYMQRKTLSMEASTAIFNEDMNRKMKFLVRKNRPD 105
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Fly
Match: mRpS21 (gene:FBgn0044511 transcript:FBtr0082764)

HSP 1 Score: 64.3142 bits (155), Expect = 8.400e-14
Identity = 28/82 (34.15%), Postives = 54/82 (65.85%), Query Frame = 2 
Query:   65 KNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPW 310
            ++ +FL R++LV +N+V+ A   L  V+  +++ ++ +   +YEKP   R R+ +E+CK IY+ +M RKI  V++ +R +P+
Sbjct:    2 RHVQFLARTVLVQNNNVEEACRLLNRVLGKEELLDQFRRTRFYEKPYQVRRRINFEKCKAIYNEDMNRKIQFVLRKNRAEPF 83
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Fly
Match: mRpS21 (gene:FBgn0044511 transcript:FBtr0335459)

HSP 1 Score: 64.3142 bits (155), Expect = 8.400e-14
Identity = 28/82 (34.15%), Postives = 54/82 (65.85%), Query Frame = 2 
Query:   65 KNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPW 310
            ++ +FL R++LV +N+V+ A   L  V+  +++ ++ +   +YEKP   R R+ +E+CK IY+ +M RKI  V++ +R +P+
Sbjct:    2 RHVQFLARTVLVQNNNVEEACRLLNRVLGKEELLDQFRRTRFYEKPYQVRRRINFEKCKAIYNEDMNRKIQFVLRKNRAEPF 83
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Zebrafish
Match: mrps21 (mitochondrial ribosomal protein S21 [Source:ZFIN;Acc:ZDB-GENE-070615-13])

HSP 1 Score: 75.8702 bits (185), Expect = 3.358e-18
Identity = 36/80 (45.00%), Postives = 51/80 (63.75%), Query Frame = 2 
Query:   74 KFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWL 313
            +F+ R+++V + +V  AY  L  V+S D I E +K + Y+EKP   + R  YE CKRIY+ EM RKI  + K  R+DPWL
Sbjct:    6 RFVSRTVMVQNGNVDLAYGFLNRVLSTDGIIESVKRRRYFEKPCRKQIRKNYENCKRIYNTEMSRKIGFIAKAQREDPWL 85
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Zebrafish
Match: mrps21 (mitochondrial ribosomal protein S21 [Source:ZFIN;Acc:ZDB-GENE-070615-13])

HSP 1 Score: 75.8702 bits (185), Expect = 3.358e-18
Identity = 36/80 (45.00%), Postives = 51/80 (63.75%), Query Frame = 2 
Query:   74 KFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWL 313
            +F+ R+++V + +V  AY  L  V+S D I E +K + Y+EKP   + R  YE CKRIY+ EM RKI  + K  R+DPWL
Sbjct:    6 RFVSRTVMVQNGNVDLAYGFLNRVLSTDGIIESVKRRRYFEKPCRKQIRKNYENCKRIYNTEMSRKIGFIAKAQREDPWL 85
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Xenopus
Match: mrps21 (mitochondrial ribosomal protein S21 [Source:NCBI gene;Acc:549094])

HSP 1 Score: 69.707 bits (169), Expect = 1.112e-15
Identity = 32/79 (40.51%), Postives = 51/79 (64.56%), Query Frame = 2 
Query:   74 KFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPW 310
            KF+ R+++V   +V  AY  L  +++ D I ++ + + YYEKP   R R  YE C+RIY+ EM  K+S +M+ +R+DPW
Sbjct:    6 KFIARTVMVPSGNVDMAYKMLNRILTVDGIVDEARRRRYYEKPCNKRRRENYENCRRIYNSEMAGKVSFLMRKNRQDPW 84
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Xenopus
Match: mrps21 (mitochondrial ribosomal protein S21 [Source:NCBI gene;Acc:549094])

HSP 1 Score: 70.0922 bits (170), Expect = 1.455e-15
Identity = 32/79 (40.51%), Postives = 51/79 (64.56%), Query Frame = 2 
Query:   74 KFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPW 310
            KF+ R+++V   +V  AY  L  +++ D I ++ + + YYEKP   R R  YE C+RIY+ EM  K+S +M+ +R+DPW
Sbjct:   24 KFIARTVMVPSGNVDMAYKMLNRILTVDGIVDEARRRRYYEKPCNKRRRENYENCRRIYNSEMAGKVSFLMRKNRQDPW 102
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Mouse
Match: Mrps21 (mitochondrial ribosomal protein S21 [Source:MGI Symbol;Acc:MGI:1913542])

HSP 1 Score: 81.2629 bits (199), Expect = 3.807e-20
Identity = 35/83 (42.17%), Postives = 56/83 (67.47%), Query Frame = 2 
Query:   65 KNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWL 313
            K+ KF+ R+++V + +V+ AY  L  +++ D ++E +  + YYEKP   R R  YE C+RIY+ EM RKI+ +M+ +R DPWL
Sbjct:    3 KHLKFIARTVMVQEGNVEGAYRTLNRILTTDGLTEVISRRRYYEKPCRRRQRESYETCRRIYNMEMARKINFLMRKNRADPWL 85
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Mouse
Match: Mrps21 (mitochondrial ribosomal protein S21 [Source:MGI Symbol;Acc:MGI:1913542])

HSP 1 Score: 81.2629 bits (199), Expect = 3.807e-20
Identity = 35/83 (42.17%), Postives = 56/83 (67.47%), Query Frame = 2 
Query:   65 KNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWL 313
            K+ KF+ R+++V + +V+ AY  L  +++ D ++E +  + YYEKP   R R  YE C+RIY+ EM RKI+ +M+ +R DPWL
Sbjct:    3 KHLKFIARTVMVQEGNVEGAYRTLNRILTTDGLTEVISRRRYYEKPCRRRQRESYETCRRIYNMEMARKINFLMRKNRADPWL 85
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. UniProt/SwissProt
Match: sp|P82921|RT21_HUMAN (28S ribosomal protein S21, mitochondrial OS=Homo sapiens OX=9606 GN=MRPS21 PE=1 SV=3)

HSP 1 Score: 82.4185 bits (202), Expect = 7.640e-20
Identity = 36/82 (43.90%), Postives = 57/82 (69.51%), Query Frame = 2 
Query:   65 KNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPW 310
            K+ KF+ R+++V + +V++AY  L  +++ D + E +K + YYEKP   R R  YERC+RIY+ EM RKI+ +M+ +R DPW
Sbjct:    3 KHLKFIARTVMVQEGNVESAYRTLNRILTMDGLIEDIKHRRYYEKPCCRRQRESYERCRRIYNMEMARKINFLMRKNRADPW 84
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. UniProt/SwissProt
Match: sp|P58059|RT21_MOUSE (28S ribosomal protein S21, mitochondrial OS=Mus musculus OX=10090 GN=Mrps21 PE=1 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 2.663e-19
Identity = 35/83 (42.17%), Postives = 56/83 (67.47%), Query Frame = 2 
Query:   65 KNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWL 313
            K+ KF+ R+++V + +V+ AY  L  +++ D ++E +  + YYEKP   R R  YE C+RIY+ EM RKI+ +M+ +R DPWL
Sbjct:    3 KHLKFIARTVMVQEGNVEGAYRTLNRILTTDGLTEVISRRRYYEKPCRRRQRESYETCRRIYNMEMARKINFLMRKNRADPWL 85
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. UniProt/SwissProt
Match: sp|P82920|RT21_BOVIN (28S ribosomal protein S21, mitochondrial OS=Bos taurus OX=9913 GN=MRPS21 PE=1 SV=3)

HSP 1 Score: 78.5666 bits (192), Expect = 2.688e-18
Identity = 35/82 (42.68%), Postives = 55/82 (67.07%), Query Frame = 2 
Query:   65 KNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPW 310
            K+ KF+ R+++V + +V+ AY  L  +++ D + E +K + YYEKP   R R  YE C+RIY+ EM RKI+ +M+ +R DPW
Sbjct:    3 KHLKFIARTVMVQEGNVEGAYRTLNRILTMDGLIEDIKRRRYYEKPCRRRQRESYETCRRIYNMEMARKINFLMRKNRADPW 84
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. TrEMBL
Match: A0A0R3SJX6 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS5239 PE=4 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 4.614e-31
Identity = 52/93 (55.91%), Postives = 72/93 (77.42%), Query Frame = 2 
Query:   38 AKSATQIYFKNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWLR 316
            +++A + YF+N +FL++++LVNDNDV +A+  L  ++  D++   +K+QEYYEKPT  R R+MYERCKRIYD EM RKI+ VMK DR DPWLR
Sbjct:    3 SRTALETYFRNVRFLRKTVLVNDNDVNSAFGALNRILRNDRVVNTVKAQEYYEKPTRMRRRIMYERCKRIYDNEMSRKINFVMKTDRPDPWLR 95
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. TrEMBL
Match: A0A068YDR1 (Mitochondrial 28S ribosomal protein S21 OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000908700 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.784e-29
Identity = 51/94 (54.26%), Postives = 70/94 (74.47%), Query Frame = 2 
Query:   35 GAKSATQIYFKNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWLR 316
             +++A + YF N +FL++++ V DNDV +A+  L  ++  DK+   +K+QEYYEKPT  R R+MYERCKRIYD EM RKI+ VMK+DR DPWLR
Sbjct:    2 ASRTALETYFNNIRFLRKTVFVKDNDVTSAFAALNKILRNDKVINVIKAQEYYEKPTRMRRRVMYERCKRIYDAEMSRKINFVMKVDRPDPWLR 95
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. TrEMBL
Match: A0A183WIR3 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS12013 PE=4 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 2.079e-29
Identity = 49/96 (51.04%), Postives = 72/96 (75.00%), Query Frame = 2 
Query:   29 MSGAKSATQIYFKNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWLR 316
            MS +     ++FKN++FLK++++V ++D   AY  L  +++ DK+   +K+QEYYE+PTVWR R+MYERCKRIYD EM RKIS+V++ +R DPW R
Sbjct:   19 MSRSTLPVDVFFKNFRFLKKTVVVKNDDYNAAYKALSKILTTDKVKNTIKAQEYYERPTVWRRRIMYERCKRIYDSEMARKISLVVRTNRIDPWPR 114
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. TrEMBL
Match: A0A068WDZ5 (Mitochondrial 28S ribosomal protein S21 OS=Echinococcus granulosus OX=6210 GN=EgrG_000908700 PE=4 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 3.245e-29
Identity = 51/94 (54.26%), Postives = 70/94 (74.47%), Query Frame = 2 
Query:   35 GAKSATQIYFKNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWLR 316
             +++A + YF N +FL++++ V DNDV +A+  L  ++  DK+   +K+QEYYEKPT  R R+MYERCKRIYD EM RKI+ VMK+DR DPWLR
Sbjct:    2 TSRTALETYFNNIRFLRKTVFVKDNDVTSAFAALNKILRNDKVINVIKAQEYYEKPTRMRRRVMYERCKRIYDAEMSRKINFVMKVDRPDPWLR 95
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. TrEMBL
Match: A0A5K3F9J4 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 5.123e-29
Identity = 50/94 (53.19%), Postives = 70/94 (74.47%), Query Frame = 2 
Query:   35 GAKSATQIYFKNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWLR 316
             +++A + YF N +FL++++LV DNDV +A+  L  ++  D++ + +K+QEYYE PT  R R+MYERCKRIYD EM RKI+ VMK DR DPWLR
Sbjct:    2 ASRTALETYFNNVRFLRKTVLVKDNDVTSAFAALNKILRNDRVIKTIKAQEYYETPTRMRRRVMYERCKRIYDSEMARKINFVMKADRPDPWLR 95
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Cavefish
Match: mrps21 (mitochondrial ribosomal protein S21 [Source:NCBI gene;Acc:103021318])

HSP 1 Score: 85.8853 bits (211), Expect = 3.645e-22
Identity = 40/80 (50.00%), Postives = 53/80 (66.25%), Query Frame = 2 
Query:   74 KFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWL 313
            +F+ R+++V D +V  AYN L  ++S D I E +K + YYEKP   R R  YE CKRIY  EM RKIS + + DR+DPWL
Sbjct:    6 RFVARTVMVRDGNVDAAYNALTRILSVDGIVETVKRKRYYEKPCRRRQRENYENCKRIYHAEMARKISFISRADRQDPWL 85
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Nematostella
Match: EDO36220 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ67])

HSP 1 Score: 57.7658 bits (138), Expect = 1.051e-11
Identity = 30/83 (36.14%), Postives = 52/83 (62.65%), Query Frame = 2 
Query:   68 NYKFLKRSLLVND-NDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKD-PW 310
            ++ FL R+++V + + V  A   L+ VM+ +K  + ++ +  YEKPT+ R R  YE   R+Y+ EM+RK+  +MK  R++ PW
Sbjct:    3 HHSFLARTIMVAEKSGVSGAIRALRNVMAQEKHLKDIQLKRRYEKPTIKRRRKTYESSLRLYNSEMQRKVDFIMKGQRRETPW 85
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Medaka
Match: mrps21 (mitochondrial ribosomal protein S21 [Source:NCBI gene;Acc:101163341])

HSP 1 Score: 75.8702 bits (185), Expect = 3.252e-18
Identity = 33/83 (39.76%), Postives = 54/83 (65.06%), Query Frame = 2 
Query:   65 KNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWL 313
            ++ +F+ R+++V D ++  AY  L  +++ D I + +K + YYEKP   R R  +E CKRIY+ EM RKI+ + + +R DPWL
Sbjct:    3 RHLRFVARTVMVQDGNIDAAYKTLNRLLTQDGIIDTVKRKRYYEKPCRERQRKNFENCKRIYNTEMARKIAFISRTNRDDPWL 85
[Close]
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Planmine SMEST
Match: SMESG000059504.1 (SMESG000059504.1)

HSP 1 Score: 196.052 bits (497), Expect = 1.779e-65
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 2 
Query:   29 MSGAKSATQIYFKNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWLR 316
            MSGAKSATQIYFKNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWLR
Sbjct:    1 MSGAKSATQIYFKNYKFLKRSLLVNDNDVQNAYNRLKWVMSADKISEKLKSQEYYEKPTVWRNRMMYERCKRIYDYEMRRKISMVMKLDRKDPWLR 96
The following BLAST results are available for this feature:
BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
MRPS211.591e-2043.90mitochondrial ribosomal protein S21 [Source:HGNC S... [more]
MRPS211.591e-2043.90mitochondrial ribosomal protein S21 [Source:HGNC S... [more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps-215.227e-725.30Mitochondrial Ribosomal Protein, Small [Source:Un... [more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mRpS218.400e-1434.15gene:FBgn0044511 transcript:FBtr0082764[more]
mRpS218.400e-1434.15gene:FBgn0044511 transcript:FBtr0335459[more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrps213.358e-1845.00mitochondrial ribosomal protein S21 [Source:ZFIN;A... [more]
mrps213.358e-1845.00mitochondrial ribosomal protein S21 [Source:ZFIN;A... [more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrps211.112e-1540.51mitochondrial ribosomal protein S21 [Source:NCBI g... [more]
mrps211.455e-1540.51mitochondrial ribosomal protein S21 [Source:NCBI g... [more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mrps213.807e-2042.17mitochondrial ribosomal protein S21 [Source:MGI Sy... [more]
Mrps213.807e-2042.17mitochondrial ribosomal protein S21 [Source:MGI Sy... [more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|P82921|RT21_HUMAN7.640e-2043.9028S ribosomal protein S21, mitochondrial OS=Homo s... [more]
sp|P58059|RT21_MOUSE2.663e-1942.1728S ribosomal protein S21, mitochondrial OS=Mus mu... [more]
sp|P82920|RT21_BOVIN2.688e-1842.6828S ribosomal protein S21, mitochondrial OS=Bos ta... [more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3SJX64.614e-3155.91Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A068YDR11.784e-2954.26Mitochondrial 28S ribosomal protein S21 OS=Echinoc... [more]
A0A183WIR32.079e-2951.04Uncharacterized protein OS=Trichobilharzia regenti... [more]
A0A068WDZ53.245e-2954.26Mitochondrial 28S ribosomal protein S21 OS=Echinoc... [more]
A0A5K3F9J45.123e-2953.19Uncharacterized protein OS=Mesocestoides corti OX=... [more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps213.645e-2250.00mitochondrial ribosomal protein S21 [Source:NCBI g... [more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO362201.051e-1136.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps213.252e-1839.76mitochondrial ribosomal protein S21 [Source:NCBI g... [more]
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BLAST of Mitochondrial 28S ribosomal protein S21 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000059504.11.779e-65100.00SMESG000059504.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035873 ID=SMED30035873|Name=Mitochondrial 28S ribosomal protein S21|organism=Schmidtea mediterranea sexual|type=transcript|length=544bp
TTCATTTTATCAGTTTTGTTTAAAATAAATGTCTGGTGCCAAAAGCGCTA
CTCAAATATACTTTAAAAATTATAAATTTTTAAAAAGATCCTTGCTAGTT
AATGACAATGACGTTCAAAATGCTTATAATCGTTTAAAATGGGTAATGAG
TGCTGACAAAATATCAGAAAAACTTAAGTCTCAAGAATATTATGAAAAAC
CAACTGTGTGGAGAAATCGTATGATGTATGAAAGATGTAAAAGAATTTAT
GATTATGAAATGCGTCGGAAAATAAGTATGGTAATGAAACTTGATCGTAA
AGACCCTTGGCTTCGTTAATTATGTGAAATAAAATTAGAAAATAAAGTGC
TAATTTATCTAATGTGTTCAAAATTTAATTAATATAAATTAAATTCTTGT
TGATTATATTTTGTGATTTATTTACATAATTTTCTTTCCGATTGTCATCA
TGTTCGTAGTCTAAAGTTTTATATTTATGAAAAATACGGTGCTTTGTTTG
ATTCCTATATTCAAACATCTATGCTAAATAATATAATTTTATTG
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000418head
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR001911Ribosomal_S21
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001911Ribosomal protein S21PFAMPF01165Ribosomal_S21coord: 24..74
e-value: 4.4E-12
score: 45.5
IPR001911Ribosomal protein S21TIGRFAMTIGR00030TIGR00030coord: 23..74
e-value: 2.8E-12
score: 44.3
IPR001911Ribosomal protein S21PANTHERPTHR21109MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S21coord: 13..94
NoneNo IPR availablePANTHERPTHR21109:SF1328S RIBOSOMAL PROTEIN S21, MITOCHONDRIALcoord: 13..94