M-phase inducer phosphatase

Overview


Name	M-phase inducer phosphatase
Smed ID	SMED30035864
Length (bp)	1645

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of M-phase inducer phosphatase (SMED30035864) t-SNE clustered cells

Violin plots show distribution of expression levels for M-phase inducer phosphatase (SMED30035864) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of M-phase inducer phosphatase (SMED30035864) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for M-phase inducer phosphatase (SMED30035864) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30035864

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30035864	SMESG000054042.1	SmedASXL_010065	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30035864	SMESG000054042.1	SmedASXL_010065	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
neoblast	SMED30035864	SMESG000054042.1	dd_Smed_v4_7176_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
testis	SMED30035864	SMESG000054042.1	KU852688	smed_ncbi_20200123	PMID:27330085 Iyer et al., 2016
male germ cell	SMED30035864	SMESG000054042.1	KU852688	smed_ncbi_20200123	PMID:27330085 Iyer et al., 2016

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Human
Match: CDC25C (cell division cycle 25C [Source:HGNC Symbol;Acc:HGNC:1727])

HSP 1 Score: 110.923 bits (276), Expect = 1.107e-25
Identity = 55/160 (34.38%), Postives = 90/160 (56.25%), Query Frame = 1

Query:  718 SLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            S  H  +  ++ +T++ LL G++   +  + +IDCR+PYEY GGHI  ++N+Y+  ++  +F +  +P +  D Q  +                  +F+CEFSS RGP++  CLR  D+  +L   P + +P +Y+L GGY +FF +Y ELC PQ Y  M
Sbjct:  223 SGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLK--KPIVPLDTQKRI----------------IIVFHCEFSSERGPRMCRCLREEDR--SLNQYPALYYPELYILKGGYRDFFPEYMELCEPQSYCPM 362

[Close]

Query:  718 SLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            S  H  +  ++ +T++ LL G++   +  + +IDCR+PYEY GGHI  ++N+Y+  ++  +F +  +P +  D Q  +                  +F+CEFSS RGP++  CLR  D+  +L   P + +P +Y+L GGY +FF +Y ELC PQ Y  M
Sbjct:  223 SGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLK--KPIVPLDTQKRI----------------IIVFHCEFSSERGPRMCRCLREEDR--SLNQYPALYYPELYILKGGYRDFFPEYMELCEPQSYCPM 362

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Human
Match: CDC25C (cell division cycle 25C [Source:HGNC Symbol;Acc:HGNC:1727])

HSP 1 Score: 110.923 bits (276), Expect = 1.807e-25
Identity = 55/160 (34.38%), Postives = 90/160 (56.25%), Query Frame = 1

Query:  718 SLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            S  H  +  ++ +T++ LL G++   +  + +IDCR+PYEY GGHI  ++N+Y+  ++  +F +  +P +  D Q  +                  +F+CEFSS RGP++  CLR  D+  +L   P + +P +Y+L GGY +FF +Y ELC PQ Y  M
Sbjct:  266 SGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLK--KPIVPLDTQKRI----------------IIVFHCEFSSERGPRMCRCLREEDR--SLNQYPALYYPELYILKGGYRDFFPEYMELCEPQSYCPM 405

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Human
Match: CDC25C (cell division cycle 25C [Source:HGNC Symbol;Acc:HGNC:1727])

HSP 1 Score: 110.923 bits (276), Expect = 1.926e-25
Identity = 55/160 (34.38%), Postives = 90/160 (56.25%), Query Frame = 1

Query:  718 SLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            S  H  +  ++ +T++ LL G++   +  + +IDCR+PYEY GGHI  ++N+Y+  ++  +F +  +P +  D Q  +                  +F+CEFSS RGP++  CLR  D+  +L   P + +P +Y+L GGY +FF +Y ELC PQ Y  M
Sbjct:  296 SGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLK--KPIVPLDTQKRI----------------IIVFHCEFSSERGPRMCRCLREEDR--SLNQYPALYYPELYILKGGYRDFFPEYMELCEPQSYCPM 435

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Human
Match: CDC25C (cell division cycle 25C [Source:HGNC Symbol;Acc:HGNC:1727])

HSP 1 Score: 110.923 bits (276), Expect = 1.926e-25
Identity = 55/160 (34.38%), Postives = 90/160 (56.25%), Query Frame = 1

Query:  718 SLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            S  H  +  ++ +T++ LL G++   +  + +IDCR+PYEY GGHI  ++N+Y+  ++  +F +  +P +  D Q  +                  +F+CEFSS RGP++  CLR  D+  +L   P + +P +Y+L GGY +FF +Y ELC PQ Y  M
Sbjct:  296 SGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLK--KPIVPLDTQKRI----------------IIVFHCEFSSERGPRMCRCLREEDR--SLNQYPALYYPELYILKGGYRDFFPEYMELCEPQSYCPM 435

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Celegans
Match: cdc-25.2 (M-phase inducer phosphatase cdc-25.2 [Source:UniProtKB/Swiss-Prot;Acc:O44628])

HSP 1 Score: 90.1225 bits (222), Expect = 5.428e-19
Identity = 57/164 (34.76%), Postives = 76/164 (46.34%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSD-YIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPE-----LCTPQGYISMKQTRYAL 1218
            I+ +TL D+          D YI+IDCR+PYEY+ GHI N+IN +                   D   V K+F   N  +     K  IFYCEFS  RGPK+A  LR +D+  N+   P+  +  +YVL  GY  FF    E     LC P  Y  M    + +
Sbjct:  226 ITAETLRDIFFRLSEKEFDDKYILIDCRYPYEYNRGHIKNAINHF-------------------DRVTVSKIFYDENGRKRC--NKIPIFYCEFSQARGPKMAYALRQVDRELNVNHYPKCDYEEMYVLDLGYRNFFFAANEANITNLCQPHAYCEMHDKEHTM 368

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Celegans
Match: cdc-25.2 (M-phase inducer phosphatase cdc-25.2 [Source:UniProtKB/Swiss-Prot;Acc:O44628])

HSP 1 Score: 88.9669 bits (219), Expect = 6.007e-19
Identity = 57/164 (34.76%), Postives = 76/164 (46.34%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSD-YIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPE-----LCTPQGYISMKQTRYAL 1218
            I+ +TL D+          D YI+IDCR+PYEY+ GHI N+IN +                   D   V K+F   N  +     K  IFYCEFS  RGPK+A  LR +D+  N+   P+  +  +YVL  GY  FF    E     LC P  Y  M    + +
Sbjct:  119 ITAETLRDIFFRLSEKEFDDKYILIDCRYPYEYNRGHIKNAINHF-------------------DRVTVSKIFYDENGRKRC--NKIPIFYCEFSQARGPKMAYALRQVDRELNVNHYPKCDYEEMYVLDLGYRNFFFAANEANITNLCQPHAYCEMHDKEHTM 261

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Celegans
Match: cdc-25.2 (M-phase inducer phosphatase cdc-25.2 [Source:UniProtKB/Swiss-Prot;Acc:O44628])

HSP 1 Score: 89.7373 bits (221), Expect = 6.039e-19
Identity = 57/164 (34.76%), Postives = 76/164 (46.34%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSD-YIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPE-----LCTPQGYISMKQTRYAL 1218
            I+ +TL D+          D YI+IDCR+PYEY+ GHI N+IN +                   D   V K+F   N  +     K  IFYCEFS  RGPK+A  LR +D+  N+   P+  +  +YVL  GY  FF    E     LC P  Y  M    + +
Sbjct:  204 ITAETLRDIFFRLSEKEFDDKYILIDCRYPYEYNRGHIKNAINHF-------------------DRVTVSKIFYDENGRKRC--NKIPIFYCEFSQARGPKMAYALRQVDRELNVNHYPKCDYEEMYVLDLGYRNFFFAANEANITNLCQPHAYCEMHDKEHTM 346

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Celegans
Match: cdc-25.1 (M-phase inducer phosphatase cdc-25.1 [Source:UniProtKB/Swiss-Prot;Acc:O44552])

HSP 1 Score: 88.5817 bits (218), Expect = 2.393e-18
Identity = 43/140 (30.71%), Postives = 70/140 (50.00%), Query Frame = 1

Query:  805 YIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYP--ELCTPQGYISMKQTRYAL 1218
            YII+DCRFP+EY GGH+  +IN++   +                   +  +F   N     F  +  IFYCE+S  RGP +A  +R +D++ N    P V++P +Y+L  GY   +      ++C P  YI M  + +++
Sbjct:  309 YIIVDCRFPFEYKGGHVKGAINLFRHDK-------------------IKPIFFPENGEASSFQNRVPIFYCEYSQKRGPTMAHAVRSIDRVLNELRYPHVEYPEMYLLDYGYKSLWSTAECRQICEPCSYIPMTHSSFSM 429

[Close]

Query:  805 YIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYP--ELCTPQGYISMKQTRYAL 1218
            YII+DCRFP+EY GGH+  +IN++   +                   +  +F   N     F  +  IFYCE+S  RGP +A  +R +D++ N    P V++P +Y+L  GY   +      ++C P  YI M  + +++
Sbjct:  309 YIIVDCRFPFEYKGGHVKGAINLFRHDK-------------------IKPIFFPENGEASSFQNRVPIFYCEYSQKRGPTMAHAVRSIDRVLNELRYPHVEYPEMYLLDYGYKSLWSTAECRQICEPCSYIPMTHSSFSM 429

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Fly
Match: stg (gene:FBgn0003525 transcript:FBtr0085397)

HSP 1 Score: 124.02 bits (310), Expect = 4.027e-30
Identity = 64/172 (37.21%), Postives = 94/172 (54.65%), Query Frame = 1

Query:  697 LPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            LP+M      H  +  IS++T++ LL G +S  ++ Y IIDCR+PYE++GGHI  + N+YT  Q++  F           L       +   ++E        IF+CEFSS RGPK++  LR LD+  N  + P + +P +Y+LH GY EFF  + ELC P  Y +M    Y
Sbjct:  287 LPLMEG---RHRDLKSISSETVARLLKGEFSDKVASYRIIDCRYPYEFEGGHIEGAKNLYTTEQILDEF-----------LTVQQTELQQQQNAESGHKRNIIIFHCEFSSERGPKMSRFLRNLDRERNTNAYPALHYPEIYLLHNGYKEFFESHVELCEPHAYRTMLDPAY 444

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Fly
Match: stg (gene:FBgn0003525 transcript:FBtr0334867)

HSP 1 Score: 124.02 bits (310), Expect = 4.027e-30
Identity = 64/172 (37.21%), Postives = 94/172 (54.65%), Query Frame = 1

Query:  697 LPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            LP+M      H  +  IS++T++ LL G +S  ++ Y IIDCR+PYE++GGHI  + N+YT  Q++  F           L       +   ++E        IF+CEFSS RGPK++  LR LD+  N  + P + +P +Y+LH GY EFF  + ELC P  Y +M    Y
Sbjct:  287 LPLMEG---RHRDLKSISSETVARLLKGEFSDKVASYRIIDCRYPYEFEGGHIEGAKNLYTTEQILDEF-----------LTVQQTELQQQQNAESGHKRNIIIFHCEFSSERGPKMSRFLRNLDRERNTNAYPALHYPEIYLLHNGYKEFFESHVELCEPHAYRTMLDPAY 444

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Fly
Match: twe (gene:FBgn0002673 transcript:FBtr0331444)

HSP 1 Score: 109.768 bits (273), Expect = 1.554e-25
Identity = 57/164 (34.76%), Postives = 86/164 (52.44%), Query Frame = 1

Query:  715 SSLNHLLIPEISNQTLSDLLVGRYSCYLS---DYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            + + H  +  IS+ TL+ L+ G +   L     Y IIDCR+PYE+ GGHI  + N+YT  Q+ + F                     P  + +  + + ++F+CEFSS RGPKL   LR  D+  +  + P + +P +Y+LH GY EFF  Y +LC P  Y+ M
Sbjct:  236 TGIRHRDLKTISSDTLARLIQGEFDEQLGSQGGYEIIDCRYPYEFLGGHIRGAKNLYTRGQIQEAF---------------------PTLTSNQENRRIYVFHCEFSSERGPKLLRYLRSNDRSQHTHNYPALDYPELYILHNGYKEFFGLYSQLCQPSQYVPM 378

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Fly
Match: twe (gene:FBgn0002673 transcript:FBtr0331445)

HSP 1 Score: 109.768 bits (273), Expect = 1.554e-25
Identity = 57/164 (34.76%), Postives = 86/164 (52.44%), Query Frame = 1

Query:  715 SSLNHLLIPEISNQTLSDLLVGRYSCYLS---DYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            + + H  +  IS+ TL+ L+ G +   L     Y IIDCR+PYE+ GGHI  + N+YT  Q+ + F                     P  + +  + + ++F+CEFSS RGPKL   LR  D+  +  + P + +P +Y+LH GY EFF  Y +LC P  Y+ M
Sbjct:  236 TGIRHRDLKTISSDTLARLIQGEFDEQLGSQGGYEIIDCRYPYEFLGGHIRGAKNLYTRGQIQEAF---------------------PTLTSNQENRRIYVFHCEFSSERGPKLLRYLRSNDRSQHTHNYPALDYPELYILHNGYKEFFGLYSQLCQPSQYVPM 378

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Fly
Match: twe (gene:FBgn0002673 transcript:FBtr0331446)

HSP 1 Score: 109.768 bits (273), Expect = 1.554e-25
Identity = 57/164 (34.76%), Postives = 86/164 (52.44%), Query Frame = 1

Query:  715 SSLNHLLIPEISNQTLSDLLVGRYSCYLS---DYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            + + H  +  IS+ TL+ L+ G +   L     Y IIDCR+PYE+ GGHI  + N+YT  Q+ + F                     P  + +  + + ++F+CEFSS RGPKL   LR  D+  +  + P + +P +Y+LH GY EFF  Y +LC P  Y+ M
Sbjct:  236 TGIRHRDLKTISSDTLARLIQGEFDEQLGSQGGYEIIDCRYPYEFLGGHIRGAKNLYTRGQIQEAF---------------------PTLTSNQENRRIYVFHCEFSSERGPKLLRYLRSNDRSQHTHNYPALDYPELYILHNGYKEFFGLYSQLCQPSQYVPM 378

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Zebrafish
Match: cdc25d (cell division cycle 25 homolog d [Source:ZFIN;Acc:ZDB-GENE-090313-74])

HSP 1 Score: 131.724 bits (330), Expect = 3.075e-33
Identity = 68/167 (40.72%), Postives = 98/167 (58.68%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQ-STQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYALRSPINPYL 1242
            IS QT++ L+ G++   + D++I+DCR+PYEY GGHI  ++N+YT  Q+ Q   Q S Q  +           +S    E+    K  IF+CEFSS RGP L + LR LD+  N+   P + +P +Y+L GGY  F   YP+LC P GY+SM+Q  Y  R  +N +L
Sbjct:  204 ISAQTVASLIRGQFGSAVEDFLIVDCRYPYEYQGGHIKGAVNLYTEHQVQQVLHQASAQVSVSTSRLCGRSASDSLPEDEESPSRKLIIFHCEFSSERGPHLCQYLRRLDRCVNV--YPSLHYPELYLLLGGYKHFHASYPDLCDPCGYVSMRQREY--REQLNSFL 366

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Zebrafish
Match: cdc25b (cell division cycle 25B [Source:ZFIN;Acc:ZDB-GENE-000330-5])

HSP 1 Score: 95.1301 bits (235), Expect = 3.230e-20
Identity = 54/156 (34.62%), Postives = 82/156 (52.56%), Query Frame = 1

Query:  733 LIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQST-QPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            + PEI  + +S    G++   +    +IDCR+PYEY+GGHI  ++N++   Q+  YF +S   PD  +  + VL                  IF+CEFSS RGP++   +R  D+  N    P + +P +Y+L GGY EFF  +  +C PQ Y  M
Sbjct:  398 ITPEIMVKAMS----GQFQDLVERLFVIDCRYPYEYEGGHIKGALNLHQEDQIEDYFLRSPILPDCPK--KRVL-----------------LIFHCEFSSERGPRMCRYVRERDRFVN--EYPNLHYPELYILKGGYKEFFQIHKAICEPQMYRPM 528

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Xenopus
Match: cdc25c (cell division cycle 25C [Source:NCBI gene;Acc:100124300])

HSP 1 Score: 113.235 bits (282), Expect = 5.367e-26
Identity = 59/151 (39.07%), Postives = 84/151 (55.63%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            I+ +TL+ L+ G +S  + +  IIDCR+PYEYDGGHI  ++N++   ++  YF +  QP      Q  L                  +F+CEFSS RGPK+   LR  D+  N    P + +P +Y+L GGY +FF +Y ELC PQGY  M
Sbjct:  385 ITAETLAALIHGDFSSLVENVFIIDCRYPYEYDGGHIKGALNLHRQEEVTDYFLK--QPLAPSVAQKRL----------------IIVFHCEFSSERGPKMCRFLREEDRARN--EYPGLYYPELYLLKGGYKDFFPEYKELCEPQGYCPM 515

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Xenopus
Match: cdc25c (cell division cycle 25C [Source:NCBI gene;Acc:100124300])

HSP 1 Score: 113.235 bits (282), Expect = 6.158e-26
Identity = 59/151 (39.07%), Postives = 84/151 (55.63%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            I+ +TL+ L+ G +S  + +  IIDCR+PYEYDGGHI  ++N++   ++  YF +  QP      Q  L                  +F+CEFSS RGPK+   LR  D+  N    P + +P +Y+L GGY +FF +Y ELC PQGY  M
Sbjct:  395 ITAETLAALIHGDFSSLVENVFIIDCRYPYEYDGGHIKGALNLHRQEEVTDYFLK--QPLAPSVAQKRL----------------IIVFHCEFSSERGPKMCRFLREEDRARN--EYPGLYYPELYLLKGGYKDFFPEYKELCEPQGYCPM 525

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BLAST of M-phase inducer phosphatase vs. Ensembl Xenopus
Match: ENSXETT00000036492.1 (M-phase inducer phosphatase 3-like [Source:NCBI gene;Acc:100497395])

HSP 1 Score: 110.153 bits (274), Expect = 1.737e-25
Identity = 64/167 (38.32%), Postives = 84/167 (50.30%), Query Frame = 1

Query:  697 LPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            LP+ R     H  +  IS  TL+ LL G Y   +  Y I+DCR+PYEY GGHI  + N+Y    +   F               LK    P S      T   IF+CEFSS R PKL   LR LD+  N    P + +P +Y+L GGY EF+ K+   C P+GY++M
Sbjct:  226 LPLERGK---HQELKYISCTTLARLLRGGYQDVVQQYQIVDCRYPYEYAGGHIKGAYNLYKEEHISDTF---------------LKNATHPKS------TTLLIFHCEFSSERAPKLCRLLRNLDR--NANRYPHLHYPELYILKGGYKEFYEKFKGFCEPRGYVNM 366

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BLAST of M-phase inducer phosphatase vs. Ensembl Xenopus
Match: man2b1 (mannosidase, alpha, class 2B, member 1 [Source:Xenbase;Acc:XB-GENE-958698])

HSP 1 Score: 109.383 bits (272), Expect = 8.151e-25
Identity = 58/160 (36.25%), Postives = 89/160 (55.62%), Query Frame = 1

Query:  718 SLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            S  H  +  I+ + +  +L GR+  ++   +IIDCR+PYEY+GGHI  +IN++  ++  +YF ++  P M    + V+                  IF+CEFSS RGP++ + LR  D+  N    P++ +P +Y+LHGGY EFF K    CTPQ Y  M
Sbjct:  354 SGRHQELKYITPELMVLILNGRFDPFIERCVIIDCRYPYEYEGGHIQGAINLHMEQEAEEYFLKN--PVMSNGSKRVI-----------------LIFHCEFSSERGPRMCKFLREKDRDRN--EYPQLYYPELYILHGGYKEFFQKCKTYCTPQTYRPM 492

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Xenopus
Match: man2b1 (mannosidase, alpha, class 2B, member 1 [Source:Xenbase;Acc:XB-GENE-958698])

HSP 1 Score: 109.383 bits (272), Expect = 8.867e-25
Identity = 58/160 (36.25%), Postives = 89/160 (55.62%), Query Frame = 1

Query:  718 SLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            S  H  +  I+ + +  +L GR+  ++   +IIDCR+PYEY+GGHI  +IN++  ++  +YF ++  P M    + V+                  IF+CEFSS RGP++ + LR  D+  N    P++ +P +Y+LHGGY EFF K    CTPQ Y  M
Sbjct:  333 SGRHQELKYITPELMVLILNGRFDPFIERCVIIDCRYPYEYEGGHIQGAINLHMEQEAEEYFLKN--PVMSNGSKRVI-----------------LIFHCEFSSERGPRMCKFLREKDRDRN--EYPQLYYPELYILHGGYKEFFQKCKTYCTPQTYRPM 471

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Mouse
Match: Cdc25b (cell division cycle 25B [Source:MGI Symbol;Acc:MGI:99701])

HSP 1 Score: 112.079 bits (279), Expect = 1.072e-25
Identity = 56/156 (35.90%), Postives = 85/156 (54.49%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            IS +T+  LL G++S  +  ++I+DCR+PYEY+GGHI N++N+   R    +  Q  +P M   L   + L                IF+CEFSS RGP++   +R  D+ +N    P + +P +Y+L GGY EFF ++P  C PQ Y  M    +
Sbjct:  385 ISPETMVALLTGKFSNIVEKFVIVDCRYPYEYEGGHIKNAVNLPLERDAETFLLQ--RPIMPCSLDKRIIL----------------IFHCEFSSERGPRMCRFIRERDRAAN--DYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAF 520

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Mouse
Match: Cdc25b (cell division cycle 25B [Source:MGI Symbol;Acc:MGI:99701])

HSP 1 Score: 112.079 bits (279), Expect = 1.179e-25
Identity = 56/156 (35.90%), Postives = 85/156 (54.49%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            IS +T+  LL G++S  +  ++I+DCR+PYEY+GGHI N++N+   R    +  Q  +P M   L   + L                IF+CEFSS RGP++   +R  D+ +N    P + +P +Y+L GGY EFF ++P  C PQ Y  M    +
Sbjct:  411 ISPETMVALLTGKFSNIVEKFVIVDCRYPYEYEGGHIKNAVNLPLERDAETFLLQ--RPIMPCSLDKRIIL----------------IFHCEFSSERGPRMCRFIRERDRAAN--DYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAF 546

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Mouse
Match: Cdc25c (cell division cycle 25C [Source:MGI Symbol;Acc:MGI:88350])

HSP 1 Score: 106.301 bits (264), Expect = 3.682e-24
Identity = 54/151 (35.76%), Postives = 86/151 (56.95%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            IS  T++ LL G++   +  + IIDCR+PYEY GGHI  ++N+++ +++ ++F +  +P +  D+Q  +                  +F CEFSS RGP++   LR  D+   L   P + +P +Y+L GGY +FF +Y ELC PQ Y  M
Sbjct:  278 ISPDTVAALLSGKFQSVIERFYIIDCRYPYEYLGGHILGALNLHSQKELHEFFLR--KPVVPLDIQKRV----------------IIVFLCEFSSERGPRMCRSLREKDR--ALNQYPALYYPELYILKGGYRDFFPEYMELCDPQSYCPM 408

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Mouse
Match: Cdc25a (cell division cycle 25A [Source:MGI Symbol;Acc:MGI:103198])

HSP 1 Score: 105.531 bits (262), Expect = 1.272e-23
Identity = 50/156 (32.05%), Postives = 87/156 (55.77%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            IS + ++ +L G+++  + +++IIDCR+PYEY+GGHI  ++N++   ++  +  ++  P +  D + V+                  +F+CEFSS RGP++   +R  D+L N    P++ +P +YVL GGY EFF+K    C P  Y  M    +
Sbjct:  350 ISPEIMASVLNGKFAGLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKN--PIVPTDGKRVI-----------------VVFHCEFSSERGPRMCRYVRERDRLGN--EYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDF 484

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Mouse
Match: Cdc25a (cell division cycle 25A [Source:MGI Symbol;Acc:MGI:103198])

HSP 1 Score: 100.138 bits (248), Expect = 3.149e-22
Identity = 46/138 (33.33%), Postives = 82/138 (59.42%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIK 1158
            IS + ++ +L G+++  + +++IIDCR+PYEY+GGHI  ++N++   ++  +  ++  P +  D + V+                  +F+CEFSS RGP++   +R  D+L N    P++ +P +YVL GGY EFF+K
Sbjct:  289 ISPEIMASVLNGKFAGLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKN--PIVPTDGKRVI-----------------VVFHCEFSSERGPRMCRYVRERDRLGN--EYPKLHYPELYVLKGGYKEFFLK 405

[Close]

BLAST of M-phase inducer phosphatase vs. UniProt/SwissProt
Match: sp|P20483|MPIP_DROME (M-phase inducer phosphatase OS=Drosophila melanogaster OX=7227 GN=stg PE=1 SV=2)

HSP 1 Score: 124.02 bits (310), Expect = 4.037e-29
Identity = 64/172 (37.21%), Postives = 94/172 (54.65%), Query Frame = 1

Query:  697 LPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            LP+M      H  +  IS++T++ LL G +S  ++ Y IIDCR+PYE++GGHI  + N+YT  Q++  F           L       +   ++E        IF+CEFSS RGPK++  LR LD+  N  + P + +P +Y+LH GY EFF  + ELC P  Y +M    Y
Sbjct:  287 LPLMEG---RHRDLKSISSETVARLLKGEFSDKVASYRIIDCRYPYEFEGGHIEGAKNLYTTEQILDEF-----------LTVQQTELQQQQNAESGHKRNIIIFHCEFSSERGPKMSRFLRNLDRERNTNAYPALHYPEIYLLHNGYKEFFESHVELCEPHAYRTMLDPAY 444

[Close]

BLAST of M-phase inducer phosphatase vs. UniProt/SwissProt
Match: sp|P30308|MPI1A_XENLA (M-phase inducer phosphatase 1-A OS=Xenopus laevis OX=8355 GN=cdc25-1-a PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 2.411e-25
Identity = 60/151 (39.74%), Postives = 85/151 (56.29%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            I+ +TL+ L+ G ++  +  + IIDCR+PYEYDGGHI ++ N++   ++  YF Q  QP     +Q  L                  IF+CEFSS RGPK+   LR  D+ SN    P + +P +Y+L GGY +FF +Y ELC PQ Y  M
Sbjct:  385 ITGETLAALMHGDFNSLVEKFFIIDCRYPYEYDGGHIKSAFNLHRQEEVTDYFLQ--QPLTPLMVQKRL----------------IIIFHCEFSSERGPKMCRFLREEDRASN--DYPSLYYPELYLLKGGYKDFFPEYKELCEPQSYCPM 515

[Close]

BLAST of M-phase inducer phosphatase vs. UniProt/SwissProt
Match: sp|P30310|MPIP2_XENLA (M-phase inducer phosphatase 2 OS=Xenopus laevis OX=8355 GN=cdc25-2 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 4.363e-25
Identity = 60/151 (39.74%), Postives = 85/151 (56.29%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            I+ +TL+ L+ G ++  +  + IIDCR+PYEYDGGHI ++ N++   ++  YF Q  QP     +Q  L                  IF+CEFSS RGPK+   LR  D+ SN    P + +P +Y+L GGY +FF +Y ELC PQ Y  M
Sbjct:  434 ITGETLAALMHGDFNSLVEKFFIIDCRYPYEYDGGHIKSAFNLHRQEEVTDYFLQ--QPLTPLMVQKRL----------------IIIFHCEFSSERGPKMCRFLREEDRASN--DYPSLYYPELYLLKGGYKDFFPEYKELCEPQSYCPM 564

[Close]

BLAST of M-phase inducer phosphatase vs. UniProt/SwissProt
Match: sp|P30311|MPIP3_XENLA (M-phase inducer phosphatase 3 OS=Xenopus laevis OX=8355 GN=cdc25-3 PE=1 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 6.809e-25
Identity = 60/151 (39.74%), Postives = 84/151 (55.63%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            I+ +TL+ L+ G ++  +  + IIDCR+PYEYDGGHI ++ N++   ++  YF Q  QP      Q  L                  IF+CEFSS RGPK+   LR  D+ SN    P + +P +Y+L GGY +FF +Y ELC PQ Y  M
Sbjct:  404 ITGETLAALMHGDFNSLVEKFFIIDCRYPYEYDGGHIKSAFNLHRQDEVTDYFLQ--QPLTPLMAQKRL----------------IIIFHCEFSSERGPKMCRSLREEDRASN--DYPSLYYPELYLLKGGYKDFFPEYKELCEPQSYCPM 534

[Close]

BLAST of M-phase inducer phosphatase vs. UniProt/SwissProt
Match: sp|P30306|MPIP2_MOUSE (M-phase inducer phosphatase 2 OS=Mus musculus OX=10090 GN=Cdc25b PE=1 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 8.248e-25
Identity = 56/156 (35.90%), Postives = 85/156 (54.49%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            IS +T+  LL G++S  +  ++I+DCR+PYEY+GGHI N++N+   R    +  Q  +P M   L   + L                IF+CEFSS RGP++   +R  D+ +N    P + +P +Y+L GGY EFF ++P  C PQ Y  M    +
Sbjct:  411 ISPETMVALLTGKFSNIVEKFVIVDCRYPYEYEGGHIKNAVNLPLERDAETFLLQ--RPIMPCSLDKRIIL----------------IFHCEFSSERGPRMCRFIRERDRAAN--DYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAF 546

[Close]

BLAST of M-phase inducer phosphatase vs. TrEMBL
Match: A0A172RVS8 (CDC25-2 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=CDC25-2 PE=2 SV=2)

HSP 1 Score: 555.058 bits (1429), Expect = 0.000e+0
Identity = 266/267 (99.63%), Postives = 266/267 (99.63%), Query Frame = 1

Query:  643 LRQRHQRSYQKISNQLSPLPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYALRSPINPYLMANSPTKHGNLTVESDSVRVPNVLTPKLTDEVTCTLYRKQQINCQRQLRCDIKRLVDIVQTITKNND 1443
            LRQRHQR YQKISNQLSPLPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYALRSPINPYLMANSPTKHGNLTVESDSVRVPNVLTPKLTDEVTCTLYRKQQINCQRQLRCDIKRLVDIVQTITKNND
Sbjct:    1 LRQRHQRRYQKISNQLSPLPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYALRSPINPYLMANSPTKHGNLTVESDSVRVPNVLTPKLTDEVTCTLYRKQQINCQRQLRCDIKRLVDIVQTITKNND 267

[Close]

BLAST of M-phase inducer phosphatase vs. TrEMBL
Match: A0A224YSX0 (M-phase inducer phosphatase OS=Rhipicephalus zambeziensis OX=60191 PE=4 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 3.649e-32
Identity = 63/152 (41.45%), Postives = 93/152 (61.18%), Query Frame = 1

Query:  757 TLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            TL+D+L G+Y   + DY +IDCR+P+E+DGGHI N+INVY+   +M  F + +   +  D + +L                  +F+CEFSS RGPKL+  LR +D+  N E  P +K+P +YVL GGY  F+ ++ +LCTPQGY+ M+   Y
Sbjct:  200 TLADVLQGKYDSVVDDYTVIDCRYPFEFDGGHIKNAINVYSSEHLMSEFLEKSA--LTPDSRRIL------------------VFHCEFSSERGPKLSRLLREMDRQINNEQYPALKYPEIYVLEGGYKAFYEEFQDLCTPQGYVPMRHKDY 331

[Close]

BLAST of M-phase inducer phosphatase vs. TrEMBL
Match: A0A4W4EKQ8 (Cell division cycle 25 homolog d OS=Electrophorus electricus OX=8005 PE=4 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.088e-31
Identity = 68/173 (39.31%), Postives = 107/173 (61.85%), Query Frame = 1

Query:  697 LPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFD-TKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            LP+ R   + H  +  +S++T++ L+ G++S  + D++IIDCR+PYEY+GGHI  ++N+YT  Q+ Q F Q++   ++ + +         +SS       K  +F+CEFSS RGP+LA  LRGLD+  N    P + +P VY+L GGYN F+ + PELC P+GY+ M    +
Sbjct:  166 LPVER---VEHRDLHCVSSETVAALIRGQFSDVVEDFLIIDCRYPYEYNGGHIKGAVNLYTESQIQQAFLQASAGQVQINEEASSPSSSPSSSSSSSQSLRKLLVFHCEFSSERGPRLAHYLRGLDRALNALLYPVLLYPEVYLLQGGYNSFYTQCPELCVPRGYVHMHHGEF 335

[Close]

BLAST of M-phase inducer phosphatase vs. TrEMBL
Match: A0A4W4EKU8 (Cell division cycle 25 homolog d OS=Electrophorus electricus OX=8005 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.172e-31
Identity = 68/173 (39.31%), Postives = 107/173 (61.85%), Query Frame = 1

Query:  697 LPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFD-TKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            LP+ R   + H  +  +S++T++ L+ G++S  + D++IIDCR+PYEY+GGHI  ++N+YT  Q+ Q F Q++   ++ + +         +SS       K  +F+CEFSS RGP+LA  LRGLD+  N    P + +P VY+L GGYN F+ + PELC P+GY+ M    +
Sbjct:  186 LPVER---VEHRDLHCVSSETVAALIRGQFSDVVEDFLIIDCRYPYEYNGGHIKGAVNLYTESQIQQAFLQASAGQVQINEEASSPSSSPSSSSSSSQSLRKLLVFHCEFSSERGPRLAHYLRGLDRALNALLYPVLLYPEVYLLQGGYNSFYTQCPELCVPRGYVHMHHGEF 355

[Close]

BLAST of M-phase inducer phosphatase vs. TrEMBL
Match: A0A172RVQ2 (CDC25-3 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=CDC25-3 PE=2 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 3.187e-30
Identity = 76/169 (44.97%), Postives = 107/169 (63.31%), Query Frame = 1

Query:  697 LPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYI-IIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDL-QHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            LPI  E++  HL IP+IS+ TL+ L+ G ++  L++ I I DCRFPYEY  GHI NS NV+  +QM+    Q+  P  E  L +H        N   DD     ++FYCE+SS+R P LA+C+R LD++ N+++   +  P++Y+L GGY EFF  YP LC PQGY+SM
Sbjct:   66 LPITTETT-GHLFIPQISSLTLAKLISGNHT--LNNPIEIFDCRFPYEYSAGHIVNSKNVHN-KQML---LQTLFPISESTLSRHSTDDALKENLKNDD---TIYVFYCEYSSYRAPDLAQCIRELDRIVNVQA-GNLFIPNIYILTGGYREFFYSYPTLCCPQGYMSM 223

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Cavefish
Match: cdc25d (cell division cycle 25 homolog d [Source:ZFIN;Acc:ZDB-GENE-090313-74])

HSP 1 Score: 95.1301 bits (235), Expect = 2.655e-21
Identity = 67/212 (31.60%), Postives = 103/212 (48.58%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHI--SNSINVYTCRQMMQYFCQS-----TQPDMEQDLQHVLKLFESPNSS----------------------EDDFDT---KAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYALRSPINPYLMANSPTKHGNLTVE 1284
            +S QT++ L+  ++S  + D++IIDCR+PYE+ GGHI    ++N+YT  Q+ Q F QS     T P  +QD +       SP +                       +D   +   K  +F+CEFSS RGP+  + LR LD+  N ++ P + +P +Y+L GGY  F+  YP+LC P  Y+          S I     + S      +T E
Sbjct:   87 VSAQTVAGLITAQFSAVVEDFLIIDCRYPYEFKGGHIKLQGAVNLYTEHQIHQAFLQSFSNFQTSPGAQQDPR-------SPEAGAVGGVQGPGSGGSRSSAVSGEIKDGGSSCPRKLIVFHCEFSSERGPRC-QYLRELDRAVNTQNYPLLLYPELYLLQGGYKHFYTHYPDLCDPCNYVHASSGLQGAASTIQQEKGSASQQTANQITAE 290

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Cavefish
Match: CDC25B (cell division cycle 25B [Source:HGNC Symbol;Acc:HGNC:1726])

HSP 1 Score: 92.8189 bits (229), Expect = 1.535e-19
Identity = 52/171 (30.41%), Postives = 85/171 (49.71%), Query Frame = 1

Query:  700 PIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRY 1212
            P +   S  H  +  I+ + +   + G++S  +    +IDCR+PYE++GGHI  ++N++   Q+   F   +QP + Q     + L                +F+CEFSS RGP++   +R  D+  +L   P + +P +YVL GGY +FF  +   C PQ Y  M    Y
Sbjct:  376 PALPTVSGKHQDLKYITPEVMVAAMSGQFSDVVERLFVIDCRYPYEFEGGHIKGALNLHLEEQIEDRFF--SQPFLPQSPDKRVLL----------------VFHCEFSSERGPRMCRFVRERDR--DLNEYPNLHYPELYVLKGGYKDFFPLHKRECEPQDYRPMVHEDY 526

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Yeast
Match: MIH1 (Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 [Source:SGD;Acc:S000004639])

HSP 1 Score: 103.99 bits (258), Expect = 5.288e-24
Identity = 57/156 (36.54%), Postives = 85/156 (54.49%), Query Frame = 1

Query:  733 LIPEISNQTLSDLLVGRY-SCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
              P IS +TL ++L       + +   IIDCRF YEY GGHI NS+N+++ R  ++Y             + + K+  S  S+ +   T   I +CEFSS RGP LA  LR  D++ N +  P++ +P + +L GGY   F  +PELC P+ Y+ M
Sbjct:  240 FFPRISPETLKNILQNNMCESFYNSCRIIDCRFEYEYTGGHIINSVNIHS-RDELEY-------------EFIHKVLHSDTSNNNTLPT-LLIIHCEFSSHRGPSLASHLRNCDRIINQDHYPKLFYPDILILDGGYKAVFDNFPELCYPRQYVGM 380

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Nematostella
Match: EDO39841 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8Z1])

HSP 1 Score: 103.605 bits (257), Expect = 1.055e-25
Identity = 56/157 (35.67%), Postives = 86/157 (54.78%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYA 1215
            IS +T++ L+ G Y   + +Y IIDCR+P+E+DGGH+  +IN+YT  +M++ F  +  P  ++  + V+                  IF+CEFSS RGP +   LR  D+  +  S P + +P +Y+L GGY  FF    E C PQ Y+ M    Y+
Sbjct:   36 ISPETVTKLINGDYKDEIDEYYIIDCRYPFEFDGGHVKGAINIYTKDEMLKQFIDT--PKCKEGKRVVV------------------IFHCEFSSKRGPDMCRFLRNRDRDIHRPSYPSLFYPELYLLDGGYKNFFNTCKEHCEPQNYVLMLDENYS 172

[Close]

BLAST of M-phase inducer phosphatase vs. Ensembl Medaka
Match: ENSORLT00000022793.2 (cell division cycle 25B [Source:NCBI gene;Acc:101168663])

HSP 1 Score: 91.6633 bits (226), Expect = 3.345e-19
Identity = 47/151 (31.13%), Postives = 78/151 (51.66%), Query Frame = 1

Query:  745 ISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            I++  +   L G +   +   I+IDCR+PYE++GGHI  ++N +   Q+ ++               +L+    P+S E        +F+CEFSS RGP++   +R  D+   L   P++ +P +Y+L GGY +FF  +   C PQ Y  M
Sbjct:  407 ITSDMMVAALSGHFHEVVDQVIVIDCRYPYEFEGGHIKGALNFHLESQVEEF---------------LLRTPIVPSSPEKRV---VVVFHCEFSSERGPRMCRFIRERDRA--LNDYPKLHYPELYILKGGYKDFFPHFQAQCEPQAYRPM 537

[Close]

BLAST of M-phase inducer phosphatase vs. Planmine SMEST
Match: SMESG000054042.1 (SMESG000054042.1)

HSP 1 Score: 853.203 bits (2203), Expect = 0.000e+0
Identity = 455/459 (99.13%), Postives = 456/459 (99.35%), Query Frame = 1

Query:   67 FVEMNKRNSSHLATELKDSRKISKSLEVPDFDLCPSKLPDWDVSSLKTLSNSTIXXXXXXXXXXXXXMNLRSRRRCSINPFDLSSDCGDSDILEIKYSLRSKKRQLHXXXXXXXXXXXXFTSTVDGESNAWSQIDSYSMTGGDICNMYEFLDNVPNEKLMETIDKNKVHSSINEXXXXXXXXXXXXLAFCYELRQRHQRSYQKISNQLSPLPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYALRSPINPYLMANSPTKHGNLTVESDSVRVPNVLTPKLTDEVTCTLYRKQQINCQRQLRCDIKRLVDIVQTITKNND 1443
             +EMNKRNSSHLATELKDSRKISKSLEVPDFDLCPSKLPDWDVSSLKTLSNSTIDSSRDSFGSNSSGMNLRSRRRCSINPFDLSSDCGDSDILEIKYSLRSKKRQLHSKSSSIINCNKN TSTVDGESNAWSQIDSYSMTGGDICNMYEFLDNVPNEKLMETIDKNKVHSSINESDCDDSDKKKPKLAFCYELRQRHQR YQKISNQLSPLPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYALRSPINPYLMANSPTKHGNLTVESDSVRVPNVLTPKLTDEVTCTLYRKQQINCQRQLRCDIKRLVDIVQTITKNND
Sbjct:   15 ILEMNKRNSSHLATELKDSRKISKSLEVPDFDLCPSKLPDWDVSSLKTLSNSTIDSSRDSFGSNSSGMNLRSRRRCSINPFDLSSDCGDSDILEIKYSLRSKKRQLHSKSSSIINCNKNLTSTVDGESNAWSQIDSYSMTGGDICNMYEFLDNVPNEKLMETIDKNKVHSSINESDCDDSDKKKPKLAFCYELRQRHQRRYQKISNQLSPLPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYALRSPINPYLMANSPTKHGNLTVESDSVRVPNVLTPKLTDEVTCTLYRKQQINCQRQLRCDIKRLVDIVQTITKNND 473

[Close]

BLAST of M-phase inducer phosphatase vs. Planmine SMEST
Match: SMESG000065054.1 (SMESG000065054.1)

HSP 1 Score: 158.688 bits (400), Expect = 4.879e-43
Identity = 83/180 (46.11%), Postives = 124/180 (68.89%), Query Frame = 1

Query:  697 LPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFC---QS-TQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYALRS 1224
            LPI  +S+ N +LIPEIS+ TL+++L G ++   ++  I DCRFPYEY GGHI+N+INVYT +Q++Q      QS T   +E+D+ ++ K  +         D   ++FYCEFSS+R PK+A+CLRGLD+L++  S   +  P++YVL+ GY +F+ +YP+ C P+ Y+SMKQTRY LR+
Sbjct:  173 LPIASDSTHN-VLIPEISSLTLANILSGNHTL-DNNITIFDCRFPYEYSGGHIANAINVYTSQQLLQILFPIKQSCTDRKLEEDISNLNKKLKR--------DNTIYVFYCEFSSYRAPKMAQCLRGLDRLAHCGSRD-LFIPNIYVLNAGYRDFYNEYPKFCFPRNYVSMKQTRYPLRN 341

[Close]

BLAST of M-phase inducer phosphatase vs. Planmine SMEST
Match: SMESG000065054.1 (SMESG000065054.1)

HSP 1 Score: 147.132 bits (370), Expect = 6.567e-39
Identity = 77/176 (43.75%), Postives = 116/176 (65.91%), Query Frame = 1

Query:  697 LPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISMKQTRYALRS 1224
            LPI  +S+ N +LIPEIS+ TL+++L G ++   ++  I DCRFPYEY GGHI+N+IN  T R++            E+D+ ++ K  +         D   ++FYCEFSS+R PK+A+CLRGLD+L++  S   +  P++YVL+ GY +F+ +YP+ C P+ Y+SMKQTRY LR+
Sbjct:  173 LPIASDSTHN-VLIPEISSLTLANILSGNHTL-DNNITIFDCRFPYEYSGGHIANAINSCTDRKL------------EEDISNLNKKLKR--------DNTIYVFYCEFSSYRAPKMAQCLRGLDRLAHCGSRD-LFIPNIYVLNAGYRDFYNEYPKFCFPRNYVSMKQTRYPLRN 325

[Close]

BLAST of M-phase inducer phosphatase vs. Planmine SMEST
Match: SMESG000075488.1 (SMESG000075488.1)

HSP 1 Score: 125.946 bits (315), Expect = 8.407e-31
Identity = 64/171 (37.43%), Postives = 100/171 (58.48%), Query Frame = 1

Query:  724 NHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQ-------------DLQHVLKLFESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            N + I E+S   ++ ++   ++     Y IIDCR+PYEYDGGH+  ++N+YT  Q++ Y   + Q D+E              DL + +   + PNSS   + T+AFIF+CEFSS RGP++A C+R +D+  N      + FP V+++  GY EF+ K+  LC+P GY+ M
Sbjct:  267 NDIKITEVSADFVAKIIENDFTSDSYAYHIIDCRYPYEYDGGHLLGAVNLYTPWQLLNYIFLA-QNDLEYLKQPVENTEDSMVDLINTIINIKKPNSS---WKTEAFIFHCEFSSVRGPEMARCMRNMDRYVNFSCQSNLFFPKVHIMKKGYREFYSKHKSLCSPCGYVKM 433

[Close]

BLAST of M-phase inducer phosphatase vs. Planmine SMEST
Match: SMESG000025390.1 (SMESG000025390.1)

HSP 1 Score: 110.538 bits (275), Expect = 1.879e-26
Identity = 59/173 (34.10%), Postives = 96/173 (55.49%), Query Frame = 1

Query:  706 MRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCRFPYEYDGGHISNSINVYTCRQMMQYF-------CQSTQPDMEQDLQHVLKL--FESPNSSEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYVLHGGYNEFFIKYPELCTPQGYISM 1197
            + E + N + I +I    +  ++   Y+    +Y IID RFP+E++GGHI  ++N+YT   + QYF        QS + ++E  +++++ L  F S             IF+CEFSS R PK+AE LR +D+ +NL+  P + +P +++L GGY  F++   ELC P  Y  M
Sbjct:   92 ISEPTDNIITIEDIDVDRVKKIVENNYTIDGLNYCIIDSRFPFEFNGGHIIGAVNLYTPLDVFQYFFGPDQLKTQSDKDNLELVVRNIINLQKFSS---------NLNLIFHCEFSSARAPKMAEELRCIDRFANLDCYPHLFYPKIFILKGGYRAFYMNNKELCEPSSYRKM 255

The following BLAST results are available for this feature:

BLAST of M-phase inducer phosphatase vs. Ensembl Human
BLAST of M-phase inducer phosphatase vs. Ensembl Celegans
BLAST of M-phase inducer phosphatase vs. Ensembl Fly
BLAST of M-phase inducer phosphatase vs. Ensembl Zebrafish
BLAST of M-phase inducer phosphatase vs. Ensembl Xenopus
BLAST of M-phase inducer phosphatase vs. Ensembl Mouse
BLAST of M-phase inducer phosphatase vs. UniProt/SwissProt
BLAST of M-phase inducer phosphatase vs. TrEMBL
BLAST of M-phase inducer phosphatase vs. Ensembl Cavefish
BLAST of M-phase inducer phosphatase vs. Ensembl Sea Lamprey
BLAST of M-phase inducer phosphatase vs. Ensembl Yeast
BLAST of M-phase inducer phosphatase vs. Ensembl Nematostella
BLAST of M-phase inducer phosphatase vs. Ensembl Medaka
BLAST of M-phase inducer phosphatase vs. Planmine SMEST

BLAST of M-phase inducer phosphatase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CDC25C	1.107e-25	34.38	cell division cycle 25C [Source:HGNC Symbol;Acc:HG...	[more]
CDC25C	1.107e-25	34.38	cell division cycle 25C [Source:HGNC Symbol;Acc:HG...	[more]
CDC25C	1.807e-25	34.38	cell division cycle 25C [Source:HGNC Symbol;Acc:HG...	[more]
CDC25C	1.926e-25	34.38	cell division cycle 25C [Source:HGNC Symbol;Acc:HG...	[more]
CDC25C	1.926e-25	34.38	cell division cycle 25C [Source:HGNC Symbol;Acc:HG...	[more]

BLAST of M-phase inducer phosphatase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
cdc-25.2	5.428e-19	34.76	M-phase inducer phosphatase cdc-25.2 [Source:UniP...	[more]
cdc-25.2	6.007e-19	34.76	M-phase inducer phosphatase cdc-25.2 [Source:UniP...	[more]
cdc-25.2	6.039e-19	34.76	M-phase inducer phosphatase cdc-25.2 [Source:UniP...	[more]
cdc-25.1	2.393e-18	30.71	M-phase inducer phosphatase cdc-25.1 [Source:UniP...	[more]
cdc-25.1	2.393e-18	30.71	M-phase inducer phosphatase cdc-25.1 [Source:UniP...	[more]

BLAST of M-phase inducer phosphatase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
stg	4.027e-30	37.21	gene:FBgn0003525 transcript:FBtr0085397	[more]
stg	4.027e-30	37.21	gene:FBgn0003525 transcript:FBtr0334867	[more]
twe	1.554e-25	34.76	gene:FBgn0002673 transcript:FBtr0331444	[more]
twe	1.554e-25	34.76	gene:FBgn0002673 transcript:FBtr0331445	[more]
twe	1.554e-25	34.76	gene:FBgn0002673 transcript:FBtr0331446	[more]

BLAST of M-phase inducer phosphatase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
cdc25d	3.075e-33	40.72	cell division cycle 25 homolog d [Source:ZFIN;Acc:...	[more]
cdc25b	3.230e-20	34.62	cell division cycle 25B [Source:ZFIN;Acc:ZDB-GENE-...	[more]

BLAST of M-phase inducer phosphatase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
cdc25c	5.367e-26	39.07	cell division cycle 25C [Source:NCBI gene;Acc:1001...	[more]
cdc25c	6.158e-26	39.07	cell division cycle 25C [Source:NCBI gene;Acc:1001...	[more]
ENSXETT00000036492.1	1.737e-25	38.32	M-phase inducer phosphatase 3-like [Source:NCBI ge...	[more]
man2b1	8.151e-25	36.25	mannosidase, alpha, class 2B, member 1 [Source:Xen...	[more]
man2b1	8.867e-25	36.25	mannosidase, alpha, class 2B, member 1 [Source:Xen...	[more]

BLAST of M-phase inducer phosphatase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Cdc25b	1.072e-25	35.90	cell division cycle 25B [Source:MGI Symbol;Acc:MGI...	[more]
Cdc25b	1.179e-25	35.90	cell division cycle 25B [Source:MGI Symbol;Acc:MGI...	[more]
Cdc25c	3.682e-24	35.76	cell division cycle 25C [Source:MGI Symbol;Acc:MGI...	[more]
Cdc25a	1.272e-23	32.05	cell division cycle 25A [Source:MGI Symbol;Acc:MGI...	[more]
Cdc25a	3.149e-22	33.33	cell division cycle 25A [Source:MGI Symbol;Acc:MGI...	[more]

BLAST of M-phase inducer phosphatase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P20483\|MPIP_DROME	4.037e-29	37.21	M-phase inducer phosphatase OS=Drosophila melanoga...	[more]
sp\|P30308\|MPI1A_XENLA	2.411e-25	39.74	M-phase inducer phosphatase 1-A OS=Xenopus laevis ...	[more]
sp\|P30310\|MPIP2_XENLA	4.363e-25	39.74	M-phase inducer phosphatase 2 OS=Xenopus laevis OX...	[more]
sp\|P30311\|MPIP3_XENLA	6.809e-25	39.74	M-phase inducer phosphatase 3 OS=Xenopus laevis OX...	[more]
sp\|P30306\|MPIP2_MOUSE	8.248e-25	35.90	M-phase inducer phosphatase 2 OS=Mus musculus OX=1...	[more]

BLAST of M-phase inducer phosphatase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A172RVS8	0.000e+0	99.63	CDC25-2 protein (Fragment) OS=Schmidtea mediterran...	[more]
A0A224YSX0	3.649e-32	41.45	M-phase inducer phosphatase OS=Rhipicephalus zambe...	[more]
A0A4W4EKQ8	1.088e-31	39.31	Cell division cycle 25 homolog d OS=Electrophorus ...	[more]
A0A4W4EKU8	1.172e-31	39.31	Cell division cycle 25 homolog d OS=Electrophorus ...	[more]
A0A172RVQ2	3.187e-30	44.97	CDC25-3 protein (Fragment) OS=Schmidtea mediterran...	[more]

BLAST of M-phase inducer phosphatase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
cdc25d	2.655e-21	31.60	cell division cycle 25 homolog d [Source:ZFIN;Acc:...	[more]
CDC25B	1.535e-19	30.41	cell division cycle 25B [Source:HGNC Symbol;Acc:HG...	[more]

BLAST of M-phase inducer phosphatase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of M-phase inducer phosphatase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
MIH1	5.288e-24	36.54	Protein tyrosine phosphatase involved in cell cycl...	[more]

BLAST of M-phase inducer phosphatase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO39841	1.055e-25	35.67	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of M-phase inducer phosphatase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSORLT00000022793.2	3.345e-19	31.13	cell division cycle 25B [Source:NCBI gene;Acc:1011...	[more]

BLAST of M-phase inducer phosphatase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000054042.1	0.000e+0	99.13	SMESG000054042.1	[more]
SMESG000065054.1	4.879e-43	46.11	SMESG000065054.1	[more]
SMESG000065054.1	6.567e-39	43.75	SMESG000065054.1	[more]
SMESG000075488.1	8.407e-31	37.43	SMESG000075488.1	[more]
SMESG000025390.1	1.879e-26	34.10	SMESG000025390.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30035864 ID=SMED30035864|Name=M-phase inducer phosphatase|organism=Schmidtea mediterranea sexual|type=transcript|length=1645bp
ATAATTCTAAATTTATTTAACGACCTAGAATATATTGAATAATCTCATTT
TATATTGAATTTTATTTTCGTTGAAATGAATAAAAGAAATTCAAGCCATC
TAGCTACAGAATTAAAAGATTCGAGAAAAATTTCTAAATCTCTGGAAGTT
CCTGATTTTGATTTATGTCCTTCAAAGTTGCCAGATTGGGATGTATCTAG
TTTGAAAACCTTGAGCAATTCCACGATAGATAGTTCTCGAGATTCATTTG
GATCAAACTCTAGTGGCATGAATTTACGAAGTCGTCGCCGCTGCTCTATT
AACCCGTTTGACTTGTCATCAGATTGTGGCGACAGCGATATTCTAGAAAT
CAAATATTCTCTGCGCAGTAAAAAGAGGCAATTACATTCGAAATCATCTT
CGATAATAAACTGTAATAAAAATTTCACGTCTACGGTTGATGGTGAGTCA
AATGCTTGGTCTCAAATTGATTCGTATTCCATGACAGGAGGGGATATTTG
TAACATGTATGAATTTCTGGACAATGTCCCCAATGAGAAATTAATGGAAA
CTATTGATAAAAATAAAGTTCATTCTTCAATTAATGAATCCGATTGCGAT
GACTCAGATAAAAAGAAACCAAAATTGGCGTTTTGTTATGAATTGAGACA
ACGGCACCAGCGAAGTTATCAAAAAATATCAAATCAACTGTCACCGTTAC
CCATAATGCGTGAATCGTCCTTGAATCATTTATTAATTCCCGAAATATCT
AATCAAACTCTTTCTGATTTGTTAGTCGGCCGATATTCGTGTTATTTATC
AGATTATATAATAATTGATTGTCGTTTCCCGTACGAATACGACGGTGGTC
ACATTAGCAACTCCATAAATGTGTACACTTGTCGACAAATGATGCAATAT
TTCTGTCAGTCAACTCAACCTGACATGGAGCAGGATCTCCAGCACGTATT
AAAGTTATTTGAATCTCCAAATTCTTCTGAAGATGATTTTGATACGAAAG
CTTTCATCTTTTACTGTGAATTCTCATCCTTTCGAGGACCTAAATTAGCT
GAATGCCTCCGAGGACTGGATAAGCTCTCTAATCTTGAGTCTCTCCCGAG
AGTAAAATTCCCTCATGTTTACGTTTTGCATGGAGGATATAATGAGTTTT
TTATTAAATATCCAGAATTATGTACTCCACAAGGATATATTAGTATGAAA
CAGACGCGATATGCTTTACGATCACCAATAAATCCGTATTTGATGGCCAA
TTCTCCGACCAAGCATGGTAATCTCACTGTTGAATCCGACAGTGTTAGAG
TGCCCAACGTCCTCACTCCGAAACTCACCGATGAAGTTACTTGTACATTA
TATAGGAAGCAACAGATAAACTGCCAGAGACAATTACGATGTGATATTAA
GCGTCTTGTTGATATAGTGCAAACGATAACAAAAAACAATGACATTATTC
GTTTTTTTAAGTTTGAAGAAATATTTTGAAGTTTTTCATCTCTATAAATG
AATAAAGTTTTAGGTTTATTATTTCAAGTTGGTTTGCGTCCATACTTTGT
TTTTATTATAAATACATAATTTATTTCCATATGAAATGATTTTAGTTATT
AATTTTATTTAATTTGTATAAATGGTTTCTCGTTTATGAGTATGT

protein sequence of SMED30035864-orf-1

>SMED30035864-orf-1 ID=SMED30035864-orf-1|Name=SMED30035864-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=468bp
MNKRNSSHLATELKDSRKISKSLEVPDFDLCPSKLPDWDVSSLKTLSNST
IDSSRDSFGSNSSGMNLRSRRRCSINPFDLSSDCGDSDILEIKYSLRSKK
RQLHSKSSSIINCNKNFTSTVDGESNAWSQIDSYSMTGGDICNMYEFLDN
VPNEKLMETIDKNKVHSSINESDCDDSDKKKPKLAFCYELRQRHQRSYQK
ISNQLSPLPIMRESSLNHLLIPEISNQTLSDLLVGRYSCYLSDYIIIDCR
FPYEYDGGHISNSINVYTCRQMMQYFCQSTQPDMEQDLQHVLKLFESPNS
SEDDFDTKAFIFYCEFSSFRGPKLAECLRGLDKLSNLESLPRVKFPHVYV
LHGGYNEFFIKYPELCTPQGYISMKQTRYALRSPINPYLMANSPTKHGNL
TVESDSVRVPNVLTPKLTDEVTCTLYRKQQINCQRQLRCDIKRLVDIVQT
ITKNNDIIRFFKFEEIF*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:1902751	positive regulation of cell cycle G2/M phase transition
GO:0006470	protein dephosphorylation
GO:0000087	mitotic M phase
GO:0000086	G2/M transition of mitotic cell cycle
GO:0000278	mitotic cell cycle
GO:0007030	Golgi organization
GO:0007049	cell cycle
GO:0007067	mitotic nuclear division
GO:0007099	centriole replication
GO:0007346	regulation of mitotic cell cycle
GO:0007369	gastrulation
GO:0007422	peripheral nervous system development
GO:0007424	open tracheal system development
GO:0007488	histoblast morphogenesis
GO:0007498	mesoderm development
GO:0008283	cell proliferation
GO:0008284	positive regulation of cell proliferation
GO:0035335	peptidyl-tyrosine dephosphorylation
GO:0045792	negative regulation of cell size
GO:0045930	negative regulation of mitotic cell cycle
GO:0045931	positive regulation of mitotic cell cycle
GO:0045977	positive regulation of mitotic cell cycle, embryonic
GO:0046331	lateral inhibition
GO:0051301	cell division
GO:0060305	regulation of cell diameter

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000208	testis
PLANA:0000228	male germ cell
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036873	Rhodanese-like_dom_sf
IPR018247	EF_Hand_1_Ca_BS
IPR000751	MPI_Phosphatase
IPR001763	Rhodanese-like_dom

Vocabulary: **cellular component**
Term	Definition
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005694	chromosome

Vocabulary: **molecular function**
Term	Definition
GO:0004725	protein tyrosine phosphatase activity
GO:0004721	phosphoprotein phosphatase activity
GO:0008138	protein tyrosine/serine/threonine phosphatase activity
GO:0016787	hydrolase activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000751	M-phase inducer phosphatase	PRINTS	PR00716	MPIPHPHTASE	coord: 246..266 score: 66.95 coord: 224..244 score: 25.77
IPR001763	Rhodanese-like domain	SMART	SM00450	rhod_4	coord: 225..364 e-value: 7.1E-10 score: 48.8
IPR001763	Rhodanese-like domain	PFAM	PF00581	Rhodanese	coord: 244..358 e-value: 4.5E-12 score: 46.4
IPR001763	Rhodanese-like domain	PROSITE	PS50206	RHODANESE_3	coord: 240..367 score: 15.216
IPR036873	Rhodanese-like domain superfamily	GENE3D	G3DSA:3.40.250.10		coord: 193..383 e-value: 9.9E-49 score: 167.1
IPR036873	Rhodanese-like domain superfamily	SUPERFAMILY	SSF52821	Rhodanese/Cell cycle control phosphatase	coord: 221..379
None	No IPR available	PANTHER	PTHR10828:SF17	CDC25-LIKE PROTEIN PHOSPHATASE TWINE-RELATED	coord: 68..379
None	No IPR available	PANTHER	PTHR10828	M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25	coord: 68..379

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M-phase inducer phosphatase

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Owlery

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