Neuropeptide Y prohormone-5
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035859 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of Neuropeptide Y prohormone-5 vs. UniProt/SwissProt
Match: sp|Q27441|NPY_APLCA (Pro-neuropeptide Y OS=Aplysia californica OX=6500 GN=NPY PE=1 SV=1) HSP 1 Score: 44.669 bits (104), Expect = 5.392e-6 Identity = 26/78 (33.33%), Postives = 41/78 (52.56%), Query Frame = 1 Query: 91 IILLMAVLGAMWLSGIQTDDR----VDIRKSIFSSPEALRRYLLQMNEYLAIVARPRYGKRSLPIDKNRLQFDNYENY 312 +IL++ +L M + ++ D+ R F+S + LR+YL +NEY +I+ RPR+GKR K N E Y Sbjct: 4 VILVVLLLSCMAVLSVRADNSEMLAPPPRPEEFTSAQQLRQYLAALNEYYSIMGRPRFGKRGDSFRKREFFRTNGERY 81
BLAST of Neuropeptide Y prohormone-5 vs. TrEMBL
Match: E3CTI3 (Neuropeptide Y prohormone-5 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 176.407 bits (446), Expect = 2.276e-55 Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 1 Query: 61 MLIGYNCKSGIILLMAVLGAMWLSGIQTDDRVDIRKSIFSSPEALRRYLLQMNEYLAIVARPRYGKRSLPIDKNRLQFDNYENYFK 318 MLIGYNCKSGIILLMAVLGAMWLSGIQTDDRVDIRKSIFSSPEALRRYLLQMNEYLAIVARPRYGKRSLPID NRLQFDNYENYFK Sbjct: 1 MLIGYNCKSGIILLMAVLGAMWLSGIQTDDRVDIRKSIFSSPEALRRYLLQMNEYLAIVARPRYGKRSLPIDMNRLQFDNYENYFK 86
BLAST of Neuropeptide Y prohormone-5 vs. TrEMBL
Match: A0A193PF77 (Neuropeptide-28 (Fragment) OS=Dugesia japonica OX=6161 GN=DjNp28 PE=2 SV=1) HSP 1 Score: 65.4698 bits (158), Expect = 4.575e-12 Identity = 31/48 (64.58%), Postives = 38/48 (79.17%), Query Frame = 1 Query: 97 LLMAVLGAMWLSGIQTDDRVDIRKSIFSSPEALRRYLLQMNEYLAIVA 240 +++ L +++ +D RVDIRKSIFSSPEALRRYL QMNEYLAIVA Sbjct: 1 IVLVFLLSLFFQETYSDSRVDIRKSIFSSPEALRRYLQQMNEYLAIVA 48
BLAST of Neuropeptide Y prohormone-5 vs. TrEMBL
Match: E3CTL5 (Neuropeptide Y prohormone-9 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 50.447 bits (119), Expect = 7.338e-6 Identity = 21/32 (65.62%), Postives = 27/32 (84.38%), Query Frame = 1 Query: 169 SIFSSPEALRRYLLQMNEYLAIVARPRYGKRS 264 S+FS PE LR YL Q+NEY+A+ +RPRYGKR+ Sbjct: 34 SLFSGPEDLRNYLRQLNEYIALSSRPRYGKRN 65
BLAST of Neuropeptide Y prohormone-5 vs. Planmine SMEST
Match: SMESG000048959.1 (SMESG000048959.1) HSP 1 Score: 176.407 bits (446), Expect = 6.330e-59 Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 1 Query: 61 MLIGYNCKSGIILLMAVLGAMWLSGIQTDDRVDIRKSIFSSPEALRRYLLQMNEYLAIVARPRYGKRSLPIDKNRLQFDNYENYFK 318 MLIGYNCKSGIILLMAVLGAMWLSGIQTDDRVDIRKSIFSSPEALRRYLLQMNEYLAIVARPRYGKRSLPID NRLQFDNYENYFK Sbjct: 1 MLIGYNCKSGIILLMAVLGAMWLSGIQTDDRVDIRKSIFSSPEALRRYLLQMNEYLAIVARPRYGKRSLPIDMNRLQFDNYENYFK 86
BLAST of Neuropeptide Y prohormone-5 vs. Planmine SMEST
Match: SMESG000048959.1 (SMESG000048959.1) HSP 1 Score: 148.288 bits (373), Expect = 5.631e-48 Identity = 71/72 (98.61%), Postives = 71/72 (98.61%), Query Frame = 1 Query: 103 MAVLGAMWLSGIQTDDRVDIRKSIFSSPEALRRYLLQMNEYLAIVARPRYGKRSLPIDKNRLQFDNYENYFK 318 MAVLGAMWLSGIQTDDRVDIRKSIFSSPEALRRYLLQMNEYLAIVARPRYGKRSLPID NRLQFDNYENYFK Sbjct: 1 MAVLGAMWLSGIQTDDRVDIRKSIFSSPEALRRYLLQMNEYLAIVARPRYGKRSLPIDMNRLQFDNYENYFK 72
BLAST of Neuropeptide Y prohormone-5 vs. Planmine SMEST
Match: SMESG000069090.1 (SMESG000069090.1) HSP 1 Score: 45.8246 bits (107), Expect = 1.039e-7 Identity = 18/33 (54.55%), Postives = 23/33 (69.70%), Query Frame = 1 Query: 163 RKSIFSSPEALRRYLLQMNEYLAIVARPRYGKR 261 + F PE LR Y+ ++NEY AIV RPR+GKR Sbjct: 29 KPEFFDDPELLRNYIKKLNEYFAIVGRPRFGKR 61 The following BLAST results are available for this feature:
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of Neuropeptide Y prohormone-5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 3
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035859 ID=SMED30035859|Name=Neuropeptide Y prohormone-5|organism=Schmidtea mediterranea sexual|type=transcript|length=343bpback to top protein sequence of SMED30035859-orf-1 >SMED30035859-orf-1 ID=SMED30035859-orf-1|Name=SMED30035859-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp LLETELDISIFSNTKIFYRKMLIGYNCKSGIILLMAVLGAMWLSGIQTDDback to top Aliases
The feature 'Neuropeptide Y prohormone-5' has the following synonyms
Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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