Placenta specific protein 8

Overview


Name	Placenta specific protein 8
Smed ID	SMED30035840
Length (bp)	629

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Placenta specific protein 8 (SMED30035840) t-SNE clustered cells

Violin plots show distribution of expression levels for Placenta specific protein 8 (SMED30035840) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Placenta specific protein 8 (SMED30035840) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Placenta specific protein 8 (SMED30035840) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30035840

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 7

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30035840	SMESG000072304.1	Contig902	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30035840	SMESG000050216.1	Contig902	GPL15192	PMID:23079596 Forsthoefel et al., 2012
pharynx	SMED30035840	SMESG000050216.1	dd_Smed_v4_564_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30035840	SMESG000050216.1	dd_Smed_v4_564_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30035840	SMESG000050216.1	dd_Smed_v4_564_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30035840	SMESG000050216.1	dd_Smed_v6_564_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
intestinal phagocyte	SMED30035840	SMESG000050216.1	dd_Smed_v6_564_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Placenta specific protein 8 vs. Ensembl Zebrafish
Match: ponzr5 (plac8 onzin related protein 5 [Source:ZFIN;Acc:ZDB-GENE-081104-325])

HSP 1 Score: 53.1434 bits (126), Expect = 7.407e-9
Identity = 30/76 (39.47%), Postives = 42/76 (55.26%), Query Frame = 1

Query:  223 FFFGCF--YTQYLMSKYGECCCAACL-----IPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMD 429
            F F CF  +      K+GEC C   L     IPA  +A+R+  R+R+ I G++  DC  S +CG C  CQI R+M+
Sbjct:   34 FSFWCFPCFACSTARKHGECLCLPLLDGFGFIPAITLAMRVSVRSRYGIKGSICKDCAYSTFCGACSWCQISREMN 109

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BLAST of Placenta specific protein 8 vs. Ensembl Zebrafish
Match: ponzr5 (plac8 onzin related protein 5 [Source:ZFIN;Acc:ZDB-GENE-081104-325])

HSP 1 Score: 53.1434 bits (126), Expect = 7.477e-9
Identity = 30/76 (39.47%), Postives = 42/76 (55.26%), Query Frame = 1

Query:  223 FFFGCF--YTQYLMSKYGECCCAACL-----IPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMD 429
            F F CF  +      K+GEC C   L     IPA  +A+R+  R+R+ I G++  DC  S +CG C  CQI R+M+
Sbjct:   37 FSFWCFPCFACSTARKHGECLCLPLLDGFGFIPAITLAMRVSVRSRYGIKGSICKDCAYSTFCGACSWCQISREMN 112

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BLAST of Placenta specific protein 8 vs. Ensembl Zebrafish
Match: ponzr2 (plac8 onzin related protein 2 [Source:ZFIN;Acc:ZDB-GENE-050411-41])

HSP 1 Score: 51.6026 bits (122), Expect = 2.522e-8
Identity = 32/77 (41.56%), Postives = 38/77 (49.35%), Query Frame = 1

Query:  223 FFFGCF--YTQYLMSKYGECCCAACL-------IPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDM 426
            F F CF  YT      YGEC C   L       IP   M++R   R R+ I G M NDC  S +C  CV CQ+ R+M
Sbjct:   38 FAFWCFSCYTCIQARNYGECLCLPLLDIFCGGMIPPITMSIRTSMRQRYGIQGTMCNDCVLSTFCRPCVWCQMSREM 114

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Query:  223 FFFGCF--YTQYLMSKYGECCCAACL-------IPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDM 426
            F F CF  YT      YGEC C   L       IP   M++R   R R+ I G M NDC  S +C  CV CQ+ R+M
Sbjct:   38 FAFWCFSCYTCIQARNYGECLCLPLLDIFCGGMIPPITMSIRTSMRQRYGIQGTMCNDCVLSTFCRPCVWCQMSREM 114

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BLAST of Placenta specific protein 8 vs. Ensembl Zebrafish
Match: plac8l1 (PLAC8 like 1 [Source:ZFIN;Acc:ZDB-GENE-091204-165])

HSP 1 Score: 45.0542 bits (105), Expect = 8.041e-6
Identity = 28/74 (37.84%), Postives = 36/74 (48.65%), Query Frame = 1

Query:  226 FFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATK 447
            F  C     L  +YGEC C   L+P    A+R+  R R KI GN+  D  T   C    LCQ+ R+M   L T+
Sbjct:   65 FVPCCLDLSLAHQYGECMCLP-LLPGSTFAMRVGIRERFKIRGNVCEDWMTVYCCYPLALCQMIREMKRRLKTQ 137

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BLAST of Placenta specific protein 8 vs. Ensembl Xenopus
Match: ENSXETT00000008293.1 (novel protein similar to cornifelin [Source:NCBI gene;Acc:733751])

HSP 1 Score: 48.521 bits (114), Expect = 3.606e-7
Identity = 23/59 (38.98%), Postives = 34/59 (57.63%), Query Frame = 1

Query:  253 LMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMD 429
            +   YGECCC   L+   ++ALR   R R+ I G +  DC    +CG C LCQ+ R+++
Sbjct:   56 VAQDYGECCCLP-LMGGTILALRTGIRERYHIQGTICTDCLCVTFCGPCALCQMARELE 113

[Close]

BLAST of Placenta specific protein 8 vs. Ensembl Xenopus
Match: ifi35 (interferon induced protein 35 [Source:Xenbase;Acc:XB-GENE-1010748])

HSP 1 Score: 48.521 bits (114), Expect = 4.591e-7
Identity = 23/59 (38.98%), Postives = 34/59 (57.63%), Query Frame = 1

Query:  253 LMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMD 429
            +   YGECCC   L+   ++ALR   R R+ I G +  DC    +CG C LCQ+ R+++
Sbjct:   66 VAQDYGECCCLP-LMGGTILALRTGIRERYHIQGTICTDCLCVTFCGPCALCQMARELE 123

[Close]

BLAST of Placenta specific protein 8 vs. Ensembl Xenopus
Match: ifi35 (interferon induced protein 35 [Source:Xenbase;Acc:XB-GENE-1010748])

HSP 1 Score: 48.1358 bits (113), Expect = 5.803e-7
Identity = 23/59 (38.98%), Postives = 34/59 (57.63%), Query Frame = 1

Query:  253 LMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMD 429
            +   YGECCC   L+   ++ALR   R R+ I G +  DC    +CG C LCQ+ R+++
Sbjct:   64 VAQDYGECCCLP-LMGGTILALRTGIRERYHIQGTICTDCLCVTFCGPCALCQMARELE 121

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BLAST of Placenta specific protein 8 vs. Ensembl Xenopus
Match: abtb1 (ankyrin repeat and BTB (POZ) domain containing 1 [Source:Xenbase;Acc:XB-GENE-976579])

HSP 1 Score: 47.7506 bits (112), Expect = 6.420e-7
Identity = 25/67 (37.31%), Postives = 36/67 (53.73%), Query Frame = 1

Query:  226 FFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDM 426
            F  C     +   +GECCC  CL    V+A+R   R R+ I G++ ND     +CG C LCQ+ R++
Sbjct:   42 FVPCILACKVSQDFGECCCLPCLF-GSVLAVRTGIRERYHIEGSICNDWVCLSFCGQCTLCQMAREL 107

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BLAST of Placenta specific protein 8 vs. Ensembl Xenopus
Match: myo19 (myosin XIX [Source:Xenbase;Acc:XB-GENE-999897])

HSP 1 Score: 47.3654 bits (111), Expect = 1.042e-6
Identity = 22/59 (37.29%), Postives = 36/59 (61.02%), Query Frame = 1

Query:  253 LMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMD 429
            +   YGECCC   ++   ++A+R   R R++I G + NDC    +CG C LCQ+ R+++
Sbjct:   51 VAQDYGECCCLP-VLNGTIIAMRTGIRERYQIPGTICNDCVCLTFCGPCTLCQMARELN 108

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BLAST of Placenta specific protein 8 vs. UniProt/SwissProt
Match: sp|Q6DK93|CNFN_XENTR (Cornifelin homolog OS=Xenopus tropicalis OX=8364 GN=cnfn PE=3 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 3.401e-6
Identity = 25/67 (37.31%), Postives = 36/67 (53.73%), Query Frame = 1

Query:  226 FFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDM 426
            F  C     +   +GECCC  CL    V+A+R   R R+ I G++ ND     +CG C LCQ+ R++
Sbjct:   42 FVPCILACKVSQDFGECCCLPCLF-GSVLAVRTGIRERYHIEGSICNDWVCLSFCGQCTLCQMAREL 107

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BLAST of Placenta specific protein 8 vs. UniProt/SwissProt
Match: sp|Q6DK99|CNFNB_XENLA (Cornifelin homolog B OS=Xenopus laevis OX=8355 GN=cnfn-b PE=3 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 5.922e-6
Identity = 25/67 (37.31%), Postives = 35/67 (52.24%), Query Frame = 1

Query:  226 FFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDM 426
            F  C     +   YGECCC  CL    V+A+R   R R+ I G++ ND     +C  C LCQ+ R++
Sbjct:   42 FVPCILACKVSKDYGECCCLPCLF-GSVLAVRTGIRERYHIEGSICNDWVCLSFCAPCTLCQMAREL 107

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BLAST of Placenta specific protein 8 vs. TrEMBL
Match: G7Y8A7 (PLAC8-like protein 1 OS=Clonorchis sinensis OX=79923 GN=CLF_102652 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 1.331e-15
Identity = 51/150 (34.00%), Postives = 77/150 (51.33%), Query Frame = 1

Query:   34 PYNKFQEKP-------PGYGGEFQPVIQNQPYAGGX-XXXXXXXXXXNIGGRDWXXXXXXXXXXXXXXXXVFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLE 459
            PYN  + KP       P +    QP+ Q  P        V   +   + G RDW   +C C ++  +C     F      ++   YGECCC   ++P   M L ++HR+RH+I+G++  DCCT + CG C LC++ RDM +V A KG+L+
Sbjct:   12 PYNSSEAKPLYPTEMQPAFPSPAQPLYQTPPAVPTVNVIVEQPKPVVSEGYRDWKDGLCDCGNNCGNCILTALFYPCMVCHMYKLYGECCCTPLVVPMADMVLSVKHRSRHRIVGSVAGDCCTFMCCGPCALCRLYRDMTFVEALKGSLQ 161

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BLAST of Placenta specific protein 8 vs. TrEMBL
Match: A0A5J4NRE5 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0010981 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 4.116e-15
Identity = 36/80 (45.00%), Postives = 51/80 (63.75%), Query Frame = 1

Query:  220 VFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLE 459
             FF+ C    ++  +YGECCC  C+IP   M L ++HR+RH+I+G++  DCCT + C  C LC++ RDM YV    GTL 
Sbjct:   74 AFFYPCVEC-HMYQRYGECCCTPCVIPMSGMVLAVKHRSRHRIVGSISGDCCTFMCCAPCALCRLYRDMVYVEGVNGTLS 152

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BLAST of Placenta specific protein 8 vs. TrEMBL
Match: A0A0P0DI19 (Placenta specific protein 8 OS=Dugesia japonica OX=6161 GN=Plac 8 PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.069e-14
Identity = 38/79 (48.10%), Postives = 53/79 (67.09%), Query Frame = 1

Query:  220 VFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTL 456
             FF  C+     M +Y EC C++ L+P  +MALRIQHRNRH I G + +DC  S   G C +CQ+KRDM ++++TKG+L
Sbjct:   54 TFFPQCYLCCMYM-EYNECFCSSLLVPNSLMALRIQHRNRHGIKGTICDDCIKSTCYGPCTMCQLKRDMKHIISTKGSL 131

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BLAST of Placenta specific protein 8 vs. TrEMBL
Match: A0A4V3SFF2 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_004386 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.351e-14
Identity = 35/80 (43.75%), Postives = 53/80 (66.25%), Query Frame = 1

Query:  220 VFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLE 459
              F+ C    ++  +YGECCC   ++P   M L ++HR+RH+I+G++  DCCT L CG C LC++ RDM +V A KG+L+
Sbjct:   82 TLFYPCVEC-HMYKRYGECCCTPLVVPMAGMVLSVKHRSRHRIVGSVAGDCCTFLCCGPCALCRLYRDMVFVEAIKGSLQ 160

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BLAST of Placenta specific protein 8 vs. TrEMBL
Match: A0A4E0RMG0 (Placenta-specific gene 8 protein OS=Fasciola hepatica OX=6192 GN=D915_001276 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 6.884e-14
Identity = 44/104 (42.31%), Postives = 58/104 (55.77%), Query Frame = 1

Query:  151 NIGGRDWXXXXXXXXXXXXXXXXVFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLEL 462
             I  RDW   +C C  D +SC CV    C Y  Y+  +Y E  C    IP P+M LR  HR R +I+G++  DC TS +C  C LCQ+ RDM Y   T+G L++
Sbjct:   19 QIPFRDWHDGLCQCSSDWKSCACVTLCTCCYMCYMFKRYNENVCTPLFIPTPIMMLRTYHRGRERIVGSLFRDCVTSAFCPWCSLCQLDRDMKYQEITRGYLDV 122

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BLAST of Placenta specific protein 8 vs. Ensembl Cavefish
Match: ponzr6 (cornifelin homolog [Source:NCBI gene;Acc:103028345])

HSP 1 Score: 53.1434 bits (126), Expect = 7.962e-9
Identity = 28/67 (41.79%), Postives = 36/67 (53.73%), Query Frame = 1

Query:  259 SKYGECCCAAC-------LIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVL 438
             KYGEC C          LIP    ++R   R R+ I G+M +DCC S  CG+C  CQI R++ Y L
Sbjct:   52 KKYGECMCLPLVELCFGGLIPPVTYSMRSSMRERYHIQGSMCDDCCVSTCCGICAWCQIARELKYRL 118

[Close]

BLAST of Placenta specific protein 8 vs. Ensembl Cavefish
Match: ponzr6 (cornifelin homolog [Source:NCBI gene;Acc:103028345])

HSP 1 Score: 51.9878 bits (123), Expect = 2.211e-8
Identity = 27/65 (41.54%), Postives = 35/65 (53.85%), Query Frame = 1

Query:  259 SKYGECCCAAC-------LIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDY 432
             KYGEC C          LIP    ++R   R R+ I G+M +DCC S  CG+C  CQI R++ Y
Sbjct:   54 KKYGECMCLPLVELCFGGLIPPVTYSMRSSMRERYHIQGSMCDDCCVSTCCGICAWCQIARELKY 118

[Close]

Query:  259 SKYGECCCAAC-------LIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDY 432
             KYGEC C          LIP    ++R   R R+ I G+M +DCC S  CG+C  CQI R++ Y
Sbjct:   54 KKYGECMCLPLVELCFGGLIPPVTYSMRSSMRERYHIQGSMCDDCCVSTCCGICAWCQIARELKY 118

[Close]

BLAST of Placenta specific protein 8 vs. Ensembl Cavefish
Match: ponzr6 (cornifelin homolog [Source:NCBI gene;Acc:103028345])

HSP 1 Score: 51.6026 bits (122), Expect = 2.380e-8
Identity = 27/65 (41.54%), Postives = 35/65 (53.85%), Query Frame = 1

Query:  259 SKYGECCCAAC-------LIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDY 432
             KYGEC C          LIP    ++R   R R+ I G+M +DCC S  CG+C  CQI R++ Y
Sbjct:   54 KKYGECMCLPLVELCFGGLIPPVTYSMRSSMRERYHIQGSMCDDCCVSTCCGICAWCQIARELKY 118

[Close]

BLAST of Placenta specific protein 8 vs. Ensembl Cavefish
Match: ponzr6 (cornifelin homolog [Source:NCBI gene;Acc:103028345])

HSP 1 Score: 51.6026 bits (122), Expect = 2.533e-8
Identity = 27/65 (41.54%), Postives = 35/65 (53.85%), Query Frame = 1

Query:  259 SKYGECCCAAC-------LIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDY 432
             KYGEC C          LIP    ++R   R R+ I G+M +DCC S  CG+C  CQI R++ Y
Sbjct:   54 KKYGECMCLPLVELCFGGLIPPVTYSMRSSMRERYHIQGSMCDDCCVSTCCGICAWCQIARELKY 118

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BLAST of Placenta specific protein 8 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009354.1 (pep scaffold:Pmarinus_7.0:GL483774:7229:14014:-1 gene:ENSPMAG00000008464.1 transcript:ENSPMAT00000009354.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 43.5134 bits (101), Expect = 3.892e-6
Identity = 21/60 (35.00%), Postives = 33/60 (55.00%), Query Frame = 1

Query:  253 LMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDY 432
            + S +GEC C   ++P  +MALR   R RH I G + +D  +   C  C  CQ+ R++ +
Sbjct:   51 VASDFGECFCVP-MLPGALMALRTGIRERHHIEGTVFDDFVSLWCCNHCTTCQMARELRH 109

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BLAST of Placenta specific protein 8 vs. Ensembl Medaka
Match: ponzr3 (cornifelin homolog A [Source:NCBI gene;Acc:101160195])

HSP 1 Score: 45.8246 bits (107), Expect = 2.449e-6
Identity = 28/75 (37.33%), Postives = 36/75 (48.00%), Query Frame = 1

Query:  223 FFFGCF--YTQYLMSKYGECCCAACL-----IPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDM 426
            F F CF  +      K GEC C   L     IP    A+R+  R ++ I G +  DC  S +CG C  CQI R+M
Sbjct:   38 FAFWCFPCFACITSKKAGECLCLPLLDGFGCIPPITTAMRVSIRKQYGIEGTICRDCVFSFFCGPCSWCQISREM 112

[Close]

BLAST of Placenta specific protein 8 vs. Ensembl Medaka
Match: plac8.1 (placenta associated 8, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-050809-124])

HSP 1 Score: 44.669 bits (104), Expect = 3.571e-6
Identity = 24/60 (40.00%), Postives = 34/60 (56.67%), Query Frame = 1

Query:  253 LMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDY 432
            + S  GECC      P     +R  +R R+ I G+M NDC  SL+C +C  CQ+KRD+ +
Sbjct:   35 IASDMGECCMFGTGYP-----IRSVYRTRYNIKGSMCNDCLMSLFCPVCSTCQLKRDIKH 89

[Close]

BLAST of Placenta specific protein 8 vs. Ensembl Medaka
Match: ENSORLT00000028572.1 (cornifelin homolog [Source:NCBI gene;Acc:101172524])

HSP 1 Score: 44.669 bits (104), Expect = 5.961e-6
Identity = 39/115 (33.91%), Postives = 57/115 (49.57%), Query Frame = 1

Query:   82 QPVIQNQPYAGGXXXXXXXXXXXNIGGRDWXXXXXXXXXXXXXXXXVFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDM 426
             PV+ +QP AGG  T        N+   +WS+ +CSC +D   C   FF     + Y   KYGE  C  C +P  + A+R   R  + I G + ND      CGLC +C++ R++
Sbjct:    3 NPVVTHQPGAGGYGT--------NVQTGEWSTGLCSCFNDCLICALGFFCPAGLSCYTAHKYGENFCLGC-VPGGMTAMRTHMRLTYGIQGTVCNDAIMVFCCGLCEMCRMAREI 108

[Close]

BLAST of Placenta specific protein 8 vs. Planmine SMEST
Match: SMESG000050216.1 (SMESG000050216.1)

HSP 1 Score: 225.713 bits (574), Expect = 5.063e-76
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 1

Query:   25 MNDPYNKFQEKPPGYGGEFQPVIQNQPYAGGXXXXXXXXXXXNIGGRDWXXXXXXXXXXXXXXXXVFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLEL 462
            MNDPYNKFQEKPPGYGGEFQPVIQNQPYAGGQTTVIVQQTTQNIGGRDWSSPMCSCCDDMESCCCVFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLEL
Sbjct:    1 MNDPYNKFQEKPPGYGGEFQPVIQNQPYAGGQTTVIVQQTTQNIGGRDWSSPMCSCCDDMESCCCVFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLEL 146

[Close]

BLAST of Placenta specific protein 8 vs. Planmine SMEST
Match: SMESG000050229.1 (SMESG000050229.1)

HSP 1 Score: 111.694 bits (278), Expect = 4.734e-31
Identity = 82/149 (55.03%), Postives = 102/149 (68.46%), Query Frame = 1

Query:   16 SDKMNDPYNKFQEKPPGYGGEFQPVIQNQPYAGGXXXXXXXXXXXNIGGRDWXXXXXXXXXXXXXXXXVFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLEL 462
            +D  N+P     ++PP Y G    V   QP   GQTTVI+QQ   + GGR WSS +CSC DDMESCCCV      YT  L S+YGECCC++C +P  + ALRIQHRNRHKI G++LND CT+++C     CQ+KRDMDYV+ATKGTL +
Sbjct:   14 ADDANEP---ILQQPPAYPG----VSVGQPSYPGQTTVIIQQPNISSGGRLWSSGICSCFDDMESCCCVMLCPTCYTARLYSRYGECCCSSCCVPNALFALRIQHRNRHKISGSILNDMCTAVFCYCLSACQMKRDMDYVVATKGTLAM 155

[Close]

BLAST of Placenta specific protein 8 vs. Planmine SMEST
Match: SMESG000050221.1 (SMESG000050221.1)

HSP 1 Score: 105.145 bits (261), Expect = 1.155e-28
Identity = 76/150 (50.67%), Postives = 99/150 (66.00%), Query Frame = 1

Query:   19 DKMNDPYNKFQEKPPGYGGEFQPVIQNQPYAG--GXXXXXXXXXXXNIGGRDWXXXXXXXXXXXXXXXXVFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLEL 462
             +++D  +   ++PP Y G          Y G  GQTTVI+QQ + + GGR WSS +CSC DDMESCCC       Y   L S+Y EC C++C IP  + ALRIQHRNRHK+ G +LNDC T+ +C LC +CQ+KRDMDYV+ATKG+L +
Sbjct:    5 SRIDDSTDPILQQPPEYPG---ISTHQTSYVGNHGQTTVIIQQPSISTGGRLWSSGICSCFDDMESCCCTILLPTCYIGRLYSRYNECICSSCCIPNALFALRIQHRNRHKVSGTILNDCMTASWCYLCAVCQLKRDMDYVVATKGSLAM 151

[Close]

BLAST of Placenta specific protein 8 vs. Planmine SMEST
Match: SMESG000050210.1 (SMESG000050210.1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.176e-25
Identity = 44/73 (60.27%), Postives = 57/73 (78.08%), Query Frame = 1

Query:  241 YTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLE 459
            Y  ++  +Y ECCC ACL+    M LRIQHRNRHKIMG++LNDCCT+L C  C +CQ+KRDM+YV++TKG+L 
Sbjct:   66 YLCHMYCQYDECCCTACLVGCSPMILRIQHRNRHKIMGSILNDCCTTLCCMQCSICQLKRDMNYVISTKGSLH 138

[Close]

BLAST of Placenta specific protein 8 vs. Planmine SMEST
Match: SMESG000050208.1 (SMESG000050208.1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.084e-17
Identity = 35/72 (48.61%), Postives = 47/72 (65.28%), Query Frame = 1

Query:  241 YTQYLMSKYGECCCAACLIPAPVMALRIQHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTL 456
            Y  +L  +Y ECCC  C +    + L +QHRNRHKI G + NDC  S  C  C +C++KRDMD+V+ TKG+L
Sbjct:   60 YVCHLYMRYDECCCTGCCLMGNPIYLAVQHRNRHKIQGTIFNDCLESFLCYQCSICRLKRDMDHVMNTKGSL 131

The following BLAST results are available for this feature:

BLAST of Placenta specific protein 8 vs. Ensembl Human
BLAST of Placenta specific protein 8 vs. Ensembl Celegans
BLAST of Placenta specific protein 8 vs. Ensembl Fly
BLAST of Placenta specific protein 8 vs. Ensembl Zebrafish
BLAST of Placenta specific protein 8 vs. Ensembl Xenopus
BLAST of Placenta specific protein 8 vs. Ensembl Mouse
BLAST of Placenta specific protein 8 vs. UniProt/SwissProt
BLAST of Placenta specific protein 8 vs. TrEMBL
BLAST of Placenta specific protein 8 vs. Ensembl Cavefish
BLAST of Placenta specific protein 8 vs. Ensembl Sea Lamprey
BLAST of Placenta specific protein 8 vs. Ensembl Yeast
BLAST of Placenta specific protein 8 vs. Ensembl Nematostella
BLAST of Placenta specific protein 8 vs. Ensembl Medaka
BLAST of Placenta specific protein 8 vs. Planmine SMEST

BLAST of Placenta specific protein 8 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Placenta specific protein 8 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Placenta specific protein 8 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Placenta specific protein 8 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ponzr5	7.407e-9	39.47	plac8 onzin related protein 5 [Source:ZFIN;Acc:ZDB...	[more]
ponzr5	7.477e-9	39.47	plac8 onzin related protein 5 [Source:ZFIN;Acc:ZDB...	[more]
ponzr2	2.522e-8	41.56	plac8 onzin related protein 2 [Source:ZFIN;Acc:ZDB...	[more]
ponzr2	2.522e-8	41.56	plac8 onzin related protein 2 [Source:ZFIN;Acc:ZDB...	[more]
plac8l1	8.041e-6	37.84	PLAC8 like 1 [Source:ZFIN;Acc:ZDB-GENE-091204-165]	[more]

BLAST of Placenta specific protein 8 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSXETT00000008293.1	3.606e-7	38.98	novel protein similar to cornifelin [Source:NCBI g...	[more]
ifi35	4.591e-7	38.98	interferon induced protein 35 [Source:Xenbase;Acc:...	[more]
ifi35	5.803e-7	38.98	interferon induced protein 35 [Source:Xenbase;Acc:...	[more]
abtb1	6.420e-7	37.31	ankyrin repeat and BTB (POZ) domain containing 1 [...	[more]
myo19	1.042e-6	37.29	myosin XIX [Source:Xenbase;Acc:XB-GENE-999897]	[more]

BLAST of Placenta specific protein 8 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Placenta specific protein 8 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2


Match Name	E-value	Identity	Description
sp\|Q6DK93\|CNFN_XENTR	3.401e-6	37.31	Cornifelin homolog OS=Xenopus tropicalis OX=8364 G...	[more]
sp\|Q6DK99\|CNFNB_XENLA	5.922e-6	37.31	Cornifelin homolog B OS=Xenopus laevis OX=8355 GN=...	[more]

BLAST of Placenta specific protein 8 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
G7Y8A7	1.331e-15	34.00	PLAC8-like protein 1 OS=Clonorchis sinensis OX=799...	[more]
A0A5J4NRE5	4.116e-15	45.00	Uncharacterized protein OS=Paragonimus westermani ...	[more]
A0A0P0DI19	1.069e-14	48.10	Placenta specific protein 8 OS=Dugesia japonica OX...	[more]
A0A4V3SFF2	1.351e-14	43.75	Uncharacterized protein OS=Opisthorchis felineus O...	[more]
A0A4E0RMG0	6.884e-14	42.31	Placenta-specific gene 8 protein OS=Fasciola hepat...	[more]

BLAST of Placenta specific protein 8 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ponzr6	7.962e-9	41.79	cornifelin homolog [Source:NCBI gene;Acc:103028345...	[more]
ponzr6	2.211e-8	41.54	cornifelin homolog [Source:NCBI gene;Acc:103028345...	[more]
ponzr6	2.211e-8	41.54	cornifelin homolog [Source:NCBI gene;Acc:103028345...	[more]
ponzr6	2.380e-8	41.54	cornifelin homolog [Source:NCBI gene;Acc:103028345...	[more]
ponzr6	2.533e-8	41.54	cornifelin homolog [Source:NCBI gene;Acc:103028345...	[more]

BLAST of Placenta specific protein 8 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000009354.1	3.892e-6	35.00	pep scaffold:Pmarinus_7.0:GL483774:7229:14014:-1 g...	[more]

BLAST of Placenta specific protein 8 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Placenta specific protein 8 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Placenta specific protein 8 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ponzr3	2.449e-6	37.33	cornifelin homolog A [Source:NCBI gene;Acc:1011601...	[more]
plac8.1	3.571e-6	40.00	placenta associated 8, tandem duplicate 1 [Source:...	[more]
ENSORLT00000028572.1	5.961e-6	33.91	cornifelin homolog [Source:NCBI gene;Acc:101172524...	[more]

BLAST of Placenta specific protein 8 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000050216.1	5.063e-76	100.00	SMESG000050216.1	[more]
SMESG000050229.1	4.734e-31	55.03	SMESG000050229.1	[more]
SMESG000050221.1	1.155e-28	50.67	SMESG000050221.1	[more]
SMESG000050210.1	1.176e-25	60.27	SMESG000050210.1	[more]
SMESG000050208.1	1.084e-17	48.61	SMESG000050208.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30035840 ID=SMED30035840|Name=Placenta specific protein 8|organism=Schmidtea mediterranea sexual|type=transcript|length=629bp
TAAATCATTAAAGTAAGCGATAAAATGAACGATCCATATAATAAATTCCA
AGAAAAGCCGCCCGGTTATGGGGGTGAATTCCAGCCAGTAATTCAAAATC
AACCATATGCGGGGGGTCAGACGACAGTGATAGTTCAACAAACGACGCAA
AACATAGGTGGCAGAGATTGGAGTTCGCCTATGTGTTCATGTTGTGATGA
CATGGAATCATGTTGTTGCGTATTCTTTTTTGGCTGTTTTTATACTCAAT
ATCTTATGTCGAAATACGGAGAATGTTGTTGCGCTGCATGTTTAATTCCA
GCGCCCGTTATGGCTCTCCGAATTCAACATAGAAATCGACACAAAATCAT
GGGTAATATGCTAAATGATTGCTGCACCTCTCTGTATTGTGGACTGTGCG
TTCTCTGCCAAATCAAGCGAGACATGGATTATGTCCTGGCGACAAAGGGA
ACCTTGGAATTGTGAATTCAATCAGAATTTTAATGATTTTATTATTTTTT
TATTAATATTATAAAATAGAACATTAAATTTAAAAATTAGATATCTTTTT
TACTTTGAACTAAAAAACTAAAAGTATTTTTGAAAATTTTTCTATGTAAT
AATATTAAATCTGTTGAAAAACTGTTGCA

protein sequence of SMED30035840-orf-1

>SMED30035840-orf-1 ID=SMED30035840-orf-1|Name=SMED30035840-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=147bp
MNDPYNKFQEKPPGYGGEFQPVIQNQPYAGGQTTVIVQQTTQNIGGRDWS
SPMCSCCDDMESCCCVFFFGCFYTQYLMSKYGECCCAACLIPAPVMALRI
QHRNRHKIMGNMLNDCCTSLYCGLCVLCQIKRDMDYVLATKGTLEL*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000070	intestinal phagocyte
PLANA:0000420	parapharyngeal region

Vocabulary: **INTERPRO**
Term	Definition
IPR006461	PLAC_motif_containing

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006461	PLAC8 motif-containing protein	TIGRFAM	TIGR01571	TIGR01571	coord: 47..134 e-value: 5.2E-12 score: 44.4
IPR006461	PLAC8 motif-containing protein	PFAM	PF04749	PLAC8	coord: 49..132 e-value: 2.6E-11 score: 44.4
IPR006461	PLAC8 motif-containing protein	PANTHER	PTHR15907	FAMILY NOT NAMED	coord: 21..136
None	No IPR available	PANTHER	PTHR15907:SF93	PLACENTA-SPECIFIC 8, TANDEM DUPLICATE 1-RELATED	coord: 21..136

Main menu

Placenta specific protein 8

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 7

Owlery

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