Cleavage and polyadenylation specificity factor subunit 4

Overview
NameCleavage and polyadenylation specificity factor subunit 4
Smed IDSMED30035837
Length (bp)508
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cleavage and polyadenylation specificity factor subunit 4 (SMED30035837) t-SNE clustered cells

Violin plots show distribution of expression levels for Cleavage and polyadenylation specificity factor subunit 4 (SMED30035837) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035837

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30035837SMESG000073125.1 SmedASXL_014907SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Human
Match: CPSF4 (cleavage and polyadenylation specific factor 4 [Source:HGNC Symbol;Acc:HGNC:2327])

HSP 1 Score: 135.191 bits (339), Expect = 8.377e-55
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ I+A+   IKF +E  +E Q+G   LPF  M+KSG  VC F++   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.4413 bits (241), Expect = 8.377e-55
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Human
Match: CPSF4 (cleavage and polyadenylation specific factor 4 [Source:HGNC Symbol;Acc:HGNC:2327])

HSP 1 Score: 134.42 bits (337), Expect = 1.812e-54
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ I+A+   IKF +E  +E Q+G   LPF  M+KSG  VC F++   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.0561 bits (240), Expect = 1.812e-54
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Human
Match: CPSF4L (cleavage and polyadenylation specific factor 4 like [Source:HGNC Symbol;Acc:HGNC:33632])

HSP 1 Score: 119.783 bits (299), Expect = 2.768e-46
Identity = 51/99 (51.52%), Postives = 68/99 (68.69%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++A      F  E  +E Q G G LPF  M+KS   VC+F+   +C  G+ CP RH +G++ VVCKHWLRGLCKKGD C+FLH+YD+ +MPECYF+
Sbjct:    1 MQEVIAGLERFTFAFEKDVEMQKGTGLLPFQGMDKSASAVCNFFTKGLCEKGKLCPFRHDRGEKMVVCKHWLRGLCKKGDHCKFLHQYDLTRMPECYFY 99

HSP 2 Score: 84.3445 bits (207), Expect = 2.768e-46
Identity = 35/62 (56.45%), Postives = 46/62 (74.19%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            T+     F+SK+G+C NKEC FLH+ P+ K QDC WYD+GFC++GP CK RHV R+ C NY+
Sbjct:   91 TRMPECYFYSKFGDCSNKECSFLHVKPAFKSQDCPWYDQGFCKDGPLCKYRHVPRIMCLNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Human
Match: CPSF4 (cleavage and polyadenylation specific factor 4 [Source:HGNC Symbol;Acc:HGNC:2327])

HSP 1 Score: 135.961 bits (341), Expect = 4.328e-40
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ I+A+   IKF +E  +E Q+G   LPF  M+KSG  VC F++   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Human
Match: CPSF4L (cleavage and polyadenylation specific factor 4 like [Source:HGNC Symbol;Acc:HGNC:33632])

HSP 1 Score: 78.9518 bits (193), Expect = 4.675e-29
Identity = 35/63 (55.56%), Postives = 46/63 (73.02%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRN-GPHCKNRHVRRVACKNYI 503
            T+     F+SK+G+C NKEC FLH+ P+ K QDC WYD+GFC++ GP CK RHV R+ C NY+
Sbjct:   27 TRMPECYFYSKFGDCSNKECSFLHVKPAFKSQDCPWYDQGFCKDAGPLCKYRHVPRIMCLNYL 89

HSP 2 Score: 67.0106 bits (162), Expect = 4.675e-29
Identity = 27/34 (79.41%), Postives = 32/34 (94.12%), Query Frame = 2 
Query:  242 VVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            VVCKHWLRGLCKKGD C+FLH+YD+ +MPECYF+
Sbjct:    2 VVCKHWLRGLCKKGDHCKFLHQYDLTRMPECYFY 35
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Celegans
Match: cpsf-4 (Cleavage and Polyadenylation Specificity Factor [Source:UniProtKB/TrEMBL;Acc:Q7YTG9])

HSP 1 Score: 120.939 bits (302), Expect = 5.203e-50
Identity = 55/104 (52.88%), Postives = 68/104 (65.38%), Query Frame = 2 
Query:   32 FWLNKMEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            F    M  +V+N +     VE  L +Q G+ E PF  +++SG  VC      +C  G  CPLRHI G++ VVCKHWLRGLCKKGD CEFLHEYD+ KMPEC+FF
Sbjct:   10 FAPQTMTDLVSNITAPMTDVEEALFNQRGMKEAPFRDLDRSGKAVCRKNKLKMCPFGPTCPLRHIDGEKAVVCKHWLRGLCKKGDQCEFLHEYDLTKMPECFFF 113

HSP 2 Score: 94.3597 bits (233), Expect = 5.203e-50
Identity = 40/62 (64.52%), Postives = 47/62 (75.81%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     FFSKY  C N+ECPF HIDP TK++DC WYDRGFCR+GP+CK+RH RR  C NY+
Sbjct:  105 TKMPECFFFSKYSACSNRECPFRHIDPETKMKDCPWYDRGFCRHGPYCKHRHRRRAVCPNYL 166
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Celegans
Match: cpsf-4 (Cleavage and Polyadenylation Specificity Factor [Source:UniProtKB/TrEMBL;Acc:Q7YTG9])

HSP 1 Score: 120.939 bits (302), Expect = 5.203e-50
Identity = 55/104 (52.88%), Postives = 68/104 (65.38%), Query Frame = 2 
Query:   32 FWLNKMEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            F    M  +V+N +     VE  L +Q G+ E PF  +++SG  VC      +C  G  CPLRHI G++ VVCKHWLRGLCKKGD CEFLHEYD+ KMPEC+FF
Sbjct:   10 FAPQTMTDLVSNITAPMTDVEEALFNQRGMKEAPFRDLDRSGKAVCRKNKLKMCPFGPTCPLRHIDGEKAVVCKHWLRGLCKKGDQCEFLHEYDLTKMPECFFF 113

HSP 2 Score: 94.3597 bits (233), Expect = 5.203e-50
Identity = 40/62 (64.52%), Postives = 47/62 (75.81%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     FFSKY  C N+ECPF HIDP TK++DC WYDRGFCR+GP+CK+RH RR  C NY+
Sbjct:  105 TKMPECFFFSKYSACSNRECPFRHIDPETKMKDCPWYDRGFCRHGPYCKHRHRRRAVCPNYL 166
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Fly
Match: Clp (gene:FBgn0015621 transcript:FBtr0078011)

HSP 1 Score: 133.265 bits (334), Expect = 1.997e-52
Identity = 62/101 (61.39%), Postives = 73/101 (72.28%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNN--ICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++AN SG++F  E  L +QVG   LPF  M+KS   VC+F   N   C  G  CP RHI+GDRT+VCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MDILLANVSGLQFKAERDLIEQVGAIPLPFYGMDKSIAAVCNFITRNGQECDKGSACPFRHIRGDRTIVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 101

HSP 2 Score: 90.5077 bits (223), Expect = 1.997e-52
Identity = 36/62 (58.06%), Postives = 49/62 (79.03%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+S++  C NKECPFLHIDP +K++DC WY RGFCR+GPHC+++H+RRV C +Y+
Sbjct:   93 TKMPECYFYSRFNACHNKECPFLHIDPQSKVKDCPWYKRGFCRHGPHCRHQHLRRVLCMDYL 154
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Zebrafish
Match: cpsf4 (cleavage and polyadenylation specific factor 4 [Source:ZFIN;Acc:ZDB-GENE-990415-180])

HSP 1 Score: 133.265 bits (334), Expect = 1.798e-54
Identity = 59/99 (59.60%), Postives = 71/99 (71.72%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++A    IKF +E  +E Q+G   LPF  M+KSG  VC ++M   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQELIATVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEYFMRAACMKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.4413 bits (241), Expect = 1.798e-54
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPDCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Zebrafish
Match: zc3h3 (zinc finger CCCH-type containing 3 [Source:ZFIN;Acc:ZDB-GENE-030131-9399])

HSP 1 Score: 49.6766 bits (117), Expect = 8.941e-13
Identity = 23/54 (42.59%), Postives = 28/54 (51.85%), Query Frame = 2 
Query:  191 CTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGD-DCEFLHEYDMNKMPECYFFFQ 349
            C  G  CP  H   D+  VC  +LRG CKK D  C F H+    KMP C +F +
Sbjct:  708 CNHGNTCPYIH-DPDKVAVCTRFLRGTCKKTDGTCPFSHKVAKEKMPVCSYFLK 760

HSP 2 Score: 40.817 bits (94), Expect = 8.941e-13
Identity = 16/40 (40.00%), Postives = 23/40 (57.50%), Query Frame = 3 
Query:  354 GECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRH 473
            G C N  CP+ H+  S K + C+ + RG+C  G  CK +H
Sbjct:  761 GICNNSSCPYSHVYVSRKAEVCEDFVRGYCPQGDKCKKKH 800

HSP 3 Score: 46.9802 bits (110), Expect = 3.512e-6
Identity = 23/63 (36.51%), Postives = 34/63 (53.97%), Query Frame = 2 
Query:  122 GELPFS-KMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTV-VCKHWLRGLCKKGDDCEFLH 304
            G  PFS K+ K  + VC +++  IC     CP  H+   R   VC+ ++RG C +GD C+  H
Sbjct:  739 GTCPFSHKVAKEKMPVCSYFLKGICN-NSSCPYSHVYVSRKAEVCEDFVRGYCPQGDKCKKKH 800
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Xenopus
Match: ubxn7 (UBX domain protein 7 [Source:Xenbase;Acc:XB-GENE-5809953])

HSP 1 Score: 132.109 bits (331), Expect = 9.913e-54
Identity = 58/99 (58.59%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++A    I+F +E  +E Q+G   LPF  M+KSG  VC F++ + C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQELIACVDHIRFDLELAVEQQLGAQPLPFPGMDKSGAAVCEFFLKSACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.0561 bits (240), Expect = 9.913e-54
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Xenopus
Match: ubxn7 (UBX domain protein 7 [Source:Xenbase;Acc:XB-GENE-5809953])

HSP 1 Score: 132.109 bits (331), Expect = 1.009e-38
Identity = 58/99 (58.59%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++A    I+F +E  +E Q+G   LPF  M+KSG  VC F++ + C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQELIACVDHIRFDLELAVEQQLGAQPLPFPGMDKSGAAVCEFFLKSACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Xenopus
Match: cpsf4l (cleavage and polyadenylation specific factor 4-like [Source:NCBI gene;Acc:549557])

HSP 1 Score: 132.109 bits (331), Expect = 5.475e-38
Identity = 60/119 (50.42%), Postives = 75/119 (63.03%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFFFQIWRMPE*GMPFSAYRPVN 403
            M+ ++A+     F +E  +E Q G   LPF  M+KSG  VC FY+  IC  G  CP RH+ G++TVVCKHWLRGLCKKGD CEFLHEYDM +MPECYF+ +         PF    P +
Sbjct:    1 MQELIASIEKFSFDLEKDIEGQRGALLLPFQGMDKSGAAVCDFYVKGICRKGSTCPFRHLNGEKTVVCKHWLRGLCKKGDQCEFLHEYDMGRMPECYFYSKFGECSNKDCPFLHIDPAS 119

HSP 2 Score: 95.9005 bits (237), Expect = 4.386e-24
Identity = 38/55 (69.09%), Postives = 49/55 (89.09%), Query Frame = 3 
Query:  339 FFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            F+SK+GEC NK+CPFLHIDP++K++DC WYDRGFC++GP CK+RH RRV C NY+
Sbjct:   98 FYSKFGECSNKDCPFLHIDPASKVKDCPWYDRGFCKHGPACKHRHTRRVMCANYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Xenopus
Match: ubxn7 (UBX domain protein 7 [Source:Xenbase;Acc:XB-GENE-5809953])

HSP 1 Score: 131.724 bits (330), Expect = 7.318e-38
Identity = 61/117 (52.14%), Postives = 76/117 (64.96%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFFFQIWRMPE*GMPFSAYRP 397
            M+ ++A    I+F +E  +E Q+G   LPF  M+KSG  VC F++ + C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+ +         PF    P
Sbjct:    1 MQELIACVDHIRFDLELAVEQQLGAQPLPFPGMDKSGAAVCEFFLKSACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKFGECSNKECPFLHIDP 117

HSP 2 Score: 97.0561 bits (240), Expect = 1.793e-24
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Xenopus
Match: cpsf4l (cleavage and polyadenylation specific factor 4-like [Source:NCBI gene;Acc:549557])

HSP 1 Score: 101.679 bits (252), Expect = 1.981e-26
Identity = 51/119 (42.86%), Postives = 65/119 (54.62%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFFFQIWRMPE*GMPFSAYRPVN 403
            M+ ++A+     F +E  +E Q G   LPF  M+ S                  CP RH+ G++TVVCKHWLRGLCKKGD CEFLHEYDM +MPECYF+ +         PF    P +
Sbjct:    1 MQELIASIEKFSFDLEKDIEGQRGALLLPFQGMDSST-----------------CPFRHLNGEKTVVCKHWLRGLCKKGDQCEFLHEYDMGRMPECYFYSKFGECSNKDCPFLHIDPAS 102

HSP 2 Score: 95.9005 bits (237), Expect = 3.098e-24
Identity = 38/55 (69.09%), Postives = 49/55 (89.09%), Query Frame = 3 
Query:  339 FFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            F+SK+GEC NK+CPFLHIDP++K++DC WYDRGFC++GP CK+RH RRV C NY+
Sbjct:   81 FYSKFGECSNKDCPFLHIDPASKVKDCPWYDRGFCKHGPACKHRHTRRVMCANYL 135
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Mouse
Match: Cpsf4 (cleavage and polyadenylation specific factor 4 [Source:MGI Symbol;Acc:MGI:1861602])

HSP 1 Score: 134.806 bits (338), Expect = 7.350e-55
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ I+A+   IKF +E  +E Q+G   LPF  M+KSG  VC F++   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.4413 bits (241), Expect = 7.350e-55
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Mouse
Match: Cpsf4 (cleavage and polyadenylation specific factor 4 [Source:MGI Symbol;Acc:MGI:1861602])

HSP 1 Score: 134.42 bits (337), Expect = 1.464e-54
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ I+A+   IKF +E  +E Q+G   LPF  M+KSG  VC F++   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.0561 bits (240), Expect = 1.464e-54
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Mouse
Match: Cpsf4 (cleavage and polyadenylation specific factor 4 [Source:MGI Symbol;Acc:MGI:1861602])

HSP 1 Score: 134.42 bits (337), Expect = 1.476e-54
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ I+A+   IKF +E  +E Q+G   LPF  M+KSG  VC F++   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.0561 bits (240), Expect = 1.476e-54
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Mouse
Match: Cpsf4 (cleavage and polyadenylation specific factor 4 [Source:MGI Symbol;Acc:MGI:1861602])

HSP 1 Score: 107.071 bits (266), Expect = 2.121e-46
Identity = 46/66 (69.70%), Postives = 52/66 (78.79%), Query Frame = 2 
Query:  146 NKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            +KSG  VC F++   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    2 DKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 67

HSP 2 Score: 97.0561 bits (240), Expect = 2.121e-46
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   59 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 120
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Mouse
Match: Cpsf4l (cleavage and polyadenylation specific factor 4-like [Source:MGI Symbol;Acc:MGI:1277182])

HSP 1 Score: 118.242 bits (295), Expect = 1.056e-42
Identity = 52/98 (53.06%), Postives = 67/98 (68.37%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYF 340
            ME ++A   G  F  E  +E Q G G LPF  M+KS   VC+F+   +C  G  CPLRH +G++ VVCKHWLRGLC+K D C+FLH+YD++KMP CYF
Sbjct:    1 MEEVIAGLQGFTFAFELDVESQKGTGLLPFQGMDKSSSAVCNFFAKGLCVKGMLCPLRHEQGEKLVVCKHWLRGLCRKSDCCDFLHQYDVSKMPVCYF 98

HSP 2 Score: 73.1738 bits (178), Expect = 1.056e-42
Identity = 33/57 (57.89%), Postives = 40/57 (70.18%), Query Frame = 3 
Query:  339 FFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRN-GPHCKNRHVRRVACKNYIN 506
            F SK+G C NKEC FLH+ P  K+QDC WY++GFC+  GP CK RHV +V C NY  
Sbjct:   98 FHSKFGNCSNKECLFLHLKPVLKLQDCPWYNQGFCKEVGPLCKYRHVHQVLCPNYFT 154
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. UniProt/SwissProt
Match: sp|O95639|CPSF4_HUMAN (Cleavage and polyadenylation specificity factor subunit 4 OS=Homo sapiens OX=9606 GN=CPSF4 PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 3.316e-54
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ I+A+   IKF +E  +E Q+G   LPF  M+KSG  VC F++   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.4413 bits (241), Expect = 3.316e-54
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. UniProt/SwissProt
Match: sp|O19137|CPSF4_BOVIN (Cleavage and polyadenylation specificity factor subunit 4 OS=Bos taurus OX=9913 GN=CPSF4 PE=1 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 7.175e-54
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ I+A+   IKF +E  +E Q+G   LPF  M+KSG  VC F++   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.0561 bits (240), Expect = 7.175e-54
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. UniProt/SwissProt
Match: sp|Q5FVR7|CPSF4_RAT (Cleavage and polyadenylation specificity factor subunit 4 OS=Rattus norvegicus OX=10116 GN=Cpsf4 PE=2 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 7.794e-54
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ I+A+   IKF +E  +E Q+G   LPF  M+KSG  VC F++   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.0561 bits (240), Expect = 7.794e-54
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. UniProt/SwissProt
Match: sp|Q6DJP7|CPSF4_XENLA (Cleavage and polyadenylation specificity factor subunit 4 OS=Xenopus laevis OX=8355 GN=cpsf4 PE=2 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 5.234e-53
Identity = 57/99 (57.58%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++A    ++F +E  +E Q+G   LPF  M+KSG  VC F++ + C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQELIACVDHLRFDLELAVEQQLGAQPLPFPGMDKSGAAVCEFFLKSACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.4413 bits (241), Expect = 5.234e-53
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. UniProt/SwissProt
Match: sp|Q9VPT8|CPSF4_DROME (Cleavage and polyadenylation specificity factor subunit 4 OS=Drosophila melanogaster OX=7227 GN=Clp PE=1 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.344e-51
Identity = 62/101 (61.39%), Postives = 73/101 (72.28%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNN--ICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++AN SG++F  E  L +QVG   LPF  M+KS   VC+F   N   C  G  CP RHI+GDRT+VCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MDILLANVSGLQFKAERDLIEQVGAIPLPFYGMDKSIAAVCNFITRNGQECDKGSACPFRHIRGDRTIVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 101

HSP 2 Score: 90.5077 bits (223), Expect = 1.344e-51
Identity = 36/62 (58.06%), Postives = 49/62 (79.03%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+S++  C NKECPFLHIDP +K++DC WY RGFCR+GPHC+++H+RRV C +Y+
Sbjct:   93 TKMPECYFYSRFNACHNKECPFLHIDPQSKVKDCPWYKRGFCRHGPHCRHQHLRRVLCMDYL 154
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. TrEMBL
Match: G4VRQ2 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_037380 PE=4 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 4.167e-64
Identity = 72/99 (72.73%), Postives = 82/99 (82.83%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++A+ S I F +ESQLE+Q+GLG LPFS MNKSG GVC F++ N C +   CPLRHIK DRTVVCKHWLRGLCKKGDDCEFLHEYDM KMPECYFF
Sbjct:    1 MDNLIADVSDIDFIIESQLENQIGLGCLPFSNMNKSGAGVCRFFITNQCPLNNLCPLRHIKADRTVVCKHWLRGLCKKGDDCEFLHEYDMTKMPECYFF 99

HSP 2 Score: 112.849 bits (281), Expect = 4.167e-64
Identity = 50/63 (79.37%), Postives = 55/63 (87.30%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 506
            TK     FFSK+GEC NKECPFLHIDP++K+QDC WYDRGFCRNGP C+NRHVRRVACKNYIN
Sbjct:   91 TKMPECYFFSKFGECMNKECPFLHIDPASKVQDCPWYDRGFCRNGPLCRNRHVRRVACKNYIN 153
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. TrEMBL
Match: A0A183Q9N7 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 4.167e-64
Identity = 72/99 (72.73%), Postives = 82/99 (82.83%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++A+ S I F +ESQLE+Q+GLG LPFS MNKSG GVC F++ N C +   CPLRHIK DRTVVCKHWLRGLCKKGDDCEFLHEYDM KMPECYFF
Sbjct:    1 MDNLIADVSDIDFIIESQLENQIGLGCLPFSNMNKSGAGVCRFFITNQCPLNNLCPLRHIKADRTVVCKHWLRGLCKKGDDCEFLHEYDMTKMPECYFF 99

HSP 2 Score: 112.849 bits (281), Expect = 4.167e-64
Identity = 50/63 (79.37%), Postives = 55/63 (87.30%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 506
            TK     FFSK+GEC NKECPFLHIDP++K+QDC WYDRGFCRNGP C+NRHVRRVACKNYIN
Sbjct:   91 TKMPECYFFSKFGECMNKECPFLHIDPASKVQDCPWYDRGFCRNGPLCRNRHVRRVACKNYIN 153
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. TrEMBL
Match: A0A419PCI0 (Cleavage and polyadenylation specificity factor subunit 4 OS=Clonorchis sinensis OX=79923 GN=CPSF4 PE=4 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 6.910e-64
Identity = 71/99 (71.72%), Postives = 82/99 (82.83%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++A+ S I F +E+QL+ Q+GLG LPFS MNKSG GVC F++ N CT+   CPLRHIK DRTVVCKHWLRGLCKKGDDCEFLHEYDM KMPECYFF
Sbjct:    1 MDNLIADVSDIDFVIETQLDKQIGLGCLPFSNMNKSGAGVCKFFIINQCTLSNLCPLRHIKADRTVVCKHWLRGLCKKGDDCEFLHEYDMTKMPECYFF 99

HSP 2 Score: 113.62 bits (283), Expect = 6.910e-64
Identity = 50/63 (79.37%), Postives = 55/63 (87.30%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 506
            TK     FFSK+GEC NKECPFLHIDP++K+QDC WYDRGFCRNGP C+NRHVRRVACKNYIN
Sbjct:   91 TKMPECYFFSKFGECMNKECPFLHIDPASKVQDCPWYDRGFCRNGPMCRNRHVRRVACKNYIN 153
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. TrEMBL
Match: A0A074Z8G2 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_15517 PE=4 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 9.315e-64
Identity = 71/99 (71.72%), Postives = 82/99 (82.83%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++A+ S I F +E+QL+ Q+GLG LPFS MNKSG GVC F++ N CT+   CPLRHIK DRTVVCKHWLRGLCKKGDDCEFLHEYDM KMPECYFF
Sbjct:    1 MDNLIADVSDIDFVIETQLDKQIGLGCLPFSNMNKSGAGVCKFFIINQCTLSNLCPLRHIKADRTVVCKHWLRGLCKKGDDCEFLHEYDMTKMPECYFF 99

HSP 2 Score: 113.235 bits (282), Expect = 9.315e-64
Identity = 50/63 (79.37%), Postives = 55/63 (87.30%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 506
            TK     FFSK+GEC NKECPFLHIDP++K+QDC WYDRGFCRNGP C+NRHVRRVACKNYIN
Sbjct:   91 TKMPECYFFSKFGECMNKECPFLHIDPASKVQDCPWYDRGFCRNGPMCRNRHVRRVACKNYIN 153
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. TrEMBL
Match: A0A095B3Z9 (Cleavage and polyadenylation specificity factor subunit 4 (Fragment) OS=Schistosoma haematobium OX=6185 GN=MS3_10495 PE=4 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 9.472e-64
Identity = 72/99 (72.73%), Postives = 82/99 (82.83%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++A+ S I F +ESQLE+Q+GLG LPFS MNKSG GVC F++ N C +   CPLRHIK DRTVVCKHWLRGLCKKGDDCEFLHEYDM KMPECYFF
Sbjct:    1 MDNLIADVSDIDFIIESQLENQIGLGCLPFSNMNKSGAGVCRFFIINQCPLNNLCPLRHIKADRTVVCKHWLRGLCKKGDDCEFLHEYDMTKMPECYFF 99

HSP 2 Score: 112.849 bits (281), Expect = 9.472e-64
Identity = 50/63 (79.37%), Postives = 55/63 (87.30%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 506
            TK     FFSK+GEC NKECPFLHIDP++K+QDC WYDRGFCRNGP C+NRHVRRVACKNYIN
Sbjct:   91 TKMPECYFFSKFGECMNKECPFLHIDPASKVQDCPWYDRGFCRNGPLCRNRHVRRVACKNYIN 153
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Cavefish
Match: cpsf4 (cleavage and polyadenylation specific factor 4 [Source:NCBI gene;Acc:103032556])

HSP 1 Score: 135.191 bits (339), Expect = 4.706e-55
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++AN   +KF +E  +E Q+G   LPF  M+KSG  VC F+M   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQELIANVDHLKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFMKAACLKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.4413 bits (241), Expect = 4.706e-55
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPDCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Cavefish
Match: cpsf4 (cleavage and polyadenylation specific factor 4 [Source:NCBI gene;Acc:103032556])

HSP 1 Score: 135.191 bits (339), Expect = 5.690e-55
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++AN   +KF +E  +E Q+G   LPF  M+KSG  VC F+M   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQELIANVDHLKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFMKAACLKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.4413 bits (241), Expect = 5.690e-55
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPDCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Cavefish
Match: cpsf4 (cleavage and polyadenylation specific factor 4 [Source:NCBI gene;Acc:103032556])

HSP 1 Score: 136.346 bits (342), Expect = 1.550e-40
Identity = 60/99 (60.61%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++AN   +KF +E  +E Q+G   LPF  M+KSG  VC F+M   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQELIANVDHLKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFMKAACLKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Cavefish
Match: zc3h3 (zinc finger CCCH-type containing 3 [Source:ZFIN;Acc:ZDB-GENE-030131-9399])

HSP 1 Score: 49.2914 bits (116), Expect = 4.570e-13
Identity = 22/54 (40.74%), Postives = 28/54 (51.85%), Query Frame = 2 
Query:  191 CTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGD-DCEFLHEYDMNKMPECYFFFQ 349
            C  G  CP  H   D+  VC  +LRG CK+ D  C F H+    KMP C +F +
Sbjct:  772 CNRGSSCPYIH-DPDKVAVCTRFLRGTCKQTDGTCPFSHKVSKEKMPVCSYFLK 824

HSP 2 Score: 42.3578 bits (98), Expect = 4.570e-13
Identity = 17/40 (42.50%), Postives = 22/40 (55.00%), Query Frame = 3 
Query:  354 GECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRH 473
            G C N  CP+ H+  S K   CQ + RG+C  G  CK +H
Sbjct:  825 GICNNSSCPYSHVYVSRKADVCQDFIRGYCPQGEKCKKKH 864

HSP 3 Score: 46.595 bits (109), Expect = 4.089e-6
Identity = 22/63 (34.92%), Postives = 35/63 (55.56%), Query Frame = 2 
Query:  122 GELPFS-KMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTV-VCKHWLRGLCKKGDDCEFLH 304
            G  PFS K++K  + VC +++  IC     CP  H+   R   VC+ ++RG C +G+ C+  H
Sbjct:  803 GTCPFSHKVSKEKMPVCSYFLKGICN-NSSCPYSHVYVSRKADVCQDFIRGYCPQGEKCKKKH 864
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Cavefish
Match: zc3h3 (zinc finger CCCH-type containing 3 [Source:ZFIN;Acc:ZDB-GENE-030131-9399])

HSP 1 Score: 49.2914 bits (116), Expect = 4.604e-13
Identity = 22/54 (40.74%), Postives = 28/54 (51.85%), Query Frame = 2 
Query:  191 CTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGD-DCEFLHEYDMNKMPECYFFFQ 349
            C  G  CP  H   D+  VC  +LRG CK+ D  C F H+    KMP C +F +
Sbjct:  772 CNRGSSCPYIH-DPDKVAVCTRFLRGTCKQTDGTCPFSHKVSKEKMPVCSYFLK 824

HSP 2 Score: 42.3578 bits (98), Expect = 4.604e-13
Identity = 17/40 (42.50%), Postives = 22/40 (55.00%), Query Frame = 3 
Query:  354 GECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRH 473
            G C N  CP+ H+  S K   CQ + RG+C  G  CK +H
Sbjct:  825 GICNNSSCPYSHVYVSRKADVCQDFIRGYCPQGEKCKKKH 864

HSP 3 Score: 46.595 bits (109), Expect = 4.102e-6
Identity = 22/63 (34.92%), Postives = 35/63 (55.56%), Query Frame = 2 
Query:  122 GELPFS-KMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTV-VCKHWLRGLCKKGDDCEFLH 304
            G  PFS K++K  + VC +++  IC     CP  H+   R   VC+ ++RG C +G+ C+  H
Sbjct:  803 GTCPFSHKVSKEKMPVCSYFLKGICN-NSSCPYSHVYVSRKADVCQDFIRGYCPQGEKCKKKH 864
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Yeast
Match: YTH1 (Essential RNA-binding component of cleavage and polyadenylation factor; contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000006311])

HSP 1 Score: 72.7886 bits (177), Expect = 4.412e-26
Identity = 39/105 (37.14%), Postives = 51/105 (48.57%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMN----NICTMGQRCPLRHIKG--DRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M  I  + +   F  E  L  +      P          +C FY +      C  G  CP +H+       +VC+HWLRGLCKK D CE+LHEY++ KMPEC FF
Sbjct:    1 MSLIHPDTAKYPFKFEPFLRQEYSFSLDP-------DRPICEFYNSREGPKSCPRGPLCPKKHVLPIFQNKIVCRHWLRGLCKKNDQCEYLHEYNLRKMPECVFF 98

HSP 2 Score: 60.077 bits (144), Expect = 4.412e-26
Identity = 28/63 (44.44%), Postives = 41/63 (65.08%), Query Frame = 3 
Query:  321 KCQNVIFFSKYGEC-QNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 506
            K    +FFSK G C Q+ +C +LHIDP++KI  C+ Y+ GFC  G  C  RH+++V C+ Y+ 
Sbjct:   91 KMPECVFFSKNGYCTQSPDCQYLHIDPASKIPKCENYEMGFCPLGSSCPRRHIKKVFCQRYMT 153
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Nematostella
Match: EDO40074 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8B4])

HSP 1 Score: 130.954 bits (328), Expect = 3.625e-53
Identity = 57/99 (57.58%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ IVA+ + I   +E QL++Q+G   LPF  M+KS   VC F++  +C  G  CP RH  G++TVVCKHWLRGLCKKGD CEFLH+YDM KMPECYF+
Sbjct:    1 MQDIVASIADITLEIEYQLKNQIGCISLPFPGMDKSSAAVCDFFVKGLCNRGAYCPFRHTSGEKTVVCKHWLRGLCKKGDQCEFLHQYDMTKMPECYFY 99

HSP 2 Score: 93.9745 bits (232), Expect = 3.625e-53
Identity = 40/62 (64.52%), Postives = 51/62 (82.26%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NK+C +LHIDP +KI+DC WYDRGFC++GPHC++RHVRRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKKCQYLHIDPQSKIKDCPWYDRGFCKHGPHCRHRHVRRVLCINYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Nematostella
Match: EDO33924 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQV2])

HSP 1 Score: 38.1206 bits (87), Expect = 3.867e-6
Identity = 18/45 (40.00%), Postives = 27/45 (60.00%), Query Frame = 3 
Query:  339 FFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRH 473
            FF K G C N +CP+ H++ S K   C+ + +G+C  G  CK +H
Sbjct:   86 FFLK-GTCFNDDCPYSHVNVSNKAAICEDFVKGYCPLGQQCKKKH 129

HSP 2 Score: 28.4906 bits (62), Expect = 3.867e-6
Identity = 16/46 (34.78%), Postives = 21/46 (45.65%), Query Frame = 2 
Query:  191 CTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGD-DCEFLHEYDMNKM 325
            C  G +C   H    +  VC  +L+G CK  D  C F H  D  K+
Sbjct:   20 CNKGDKCKYIH-DPSKVAVCTKFLKGKCKNTDGTCTFSHRIDKEKV 64
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Medaka
Match: cpsf4 (cleavage and polyadenylation specific factor 4 [Source:NCBI gene;Acc:101173062])

HSP 1 Score: 136.732 bits (343), Expect = 1.250e-55
Identity = 61/99 (61.62%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++AN   IKF +E  +E Q+G   LPF  M+KSG  VC F+M   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQELLANVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGSAVCEFFMKGTCMKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 98.2117 bits (243), Expect = 1.250e-55
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPDCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Medaka
Match: cpsf4 (cleavage and polyadenylation specific factor 4 [Source:NCBI gene;Acc:101173062])

HSP 1 Score: 135.961 bits (341), Expect = 2.747e-55
Identity = 61/99 (61.62%), Postives = 72/99 (72.73%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ ++AN   IKF +E  +E Q+G   LPF  M+KSG  VC F+M   C  G  CP RHI G++TVVCKHWLRGLCKKGD CEFLHEYDM KMPECYF+
Sbjct:    1 MQELLANVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGSAVCEFFMKGTCMKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFY 99

HSP 2 Score: 97.8265 bits (242), Expect = 2.747e-55
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYI 503
            TK     F+SK+GEC NKECPFLHIDP +KI+DC WYDRGFC++GP C++RH RRV C NY+
Sbjct:   91 TKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPDCRHRHTRRVICVNYL 152
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Medaka
Match: zc3h3 (zinc finger CCCH-type containing 3 [Source:NCBI gene;Acc:101168697])

HSP 1 Score: 46.2098 bits (108), Expect = 4.421e-12
Identity = 21/54 (38.89%), Postives = 27/54 (50.00%), Query Frame = 2 
Query:  191 CTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGD-DCEFLHEYDMNKMPECYFFFQ 349
            C  G  C   H   D+  VC  +LRG CK+ D  C F H+    KMP C +F +
Sbjct:  653 CNRGTSCTFIH-DPDKVAVCTRFLRGTCKREDGTCPFSHKVSKEKMPVCSYFLK 705

HSP 2 Score: 41.9726 bits (97), Expect = 4.421e-12
Identity = 16/49 (32.65%), Postives = 29/49 (59.18%), Query Frame = 3 
Query:  354 GECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNY 500
            G C N +CP+ H+  S+K + C+ + +G+C  G  CK +H   + C ++
Sbjct:  706 GICNNSDCPYSHVYVSSKAEVCEDFVKGYCPEGEKCKKKHT--LVCSDF 752
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Medaka
Match: zc3h3 (zinc finger CCCH-type containing 3 [Source:NCBI gene;Acc:101168697])

HSP 1 Score: 46.2098 bits (108), Expect = 4.606e-12
Identity = 21/54 (38.89%), Postives = 27/54 (50.00%), Query Frame = 2 
Query:  191 CTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGD-DCEFLHEYDMNKMPECYFFFQ 349
            C  G  C   H   D+  VC  +LRG CK+ D  C F H+    KMP C +F +
Sbjct:  653 CNRGTSCTFIH-DPDKVAVCTRFLRGTCKREDGTCPFSHKVSKEKMPVCSYFLK 705

HSP 2 Score: 41.9726 bits (97), Expect = 4.606e-12
Identity = 16/49 (32.65%), Postives = 29/49 (59.18%), Query Frame = 3 
Query:  354 GECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNY 500
            G C N +CP+ H+  S+K + C+ + +G+C  G  CK +H   + C ++
Sbjct:  706 GICNNSDCPYSHVYVSSKAEVCEDFVKGYCPEGEKCKKKHT--LVCSDF 752
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Medaka
Match: zc3h6 (zinc finger CCCH-type containing 6 [Source:NCBI gene;Acc:101161746])

HSP 1 Score: 47.7506 bits (112), Expect = 1.798e-6
Identity = 21/67 (31.34%), Postives = 36/67 (53.73%), Query Frame = 2 
Query:  152 SGVGVCHFYMNNICTMGQRCPLRH---IKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            +G  +C +++   C  G++C   H   +   +  +CK +L+G C KGD C ++H    N+ P C FF
Sbjct:  315 NGRYICKYFLEGRCIKGEQCKFEHELVVPDKKKELCKFYLQGYCSKGDHCIYMH----NEYP-CKFF 376
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Planmine SMEST
Match: SMESG000073125.1 (SMESG000073125.1)

HSP 1 Score: 209.92 bits (533), Expect = 3.205e-85
Identity = 99/99 (100.00%), Postives = 99/99 (100.00%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF
Sbjct:    1 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 99

HSP 2 Score: 123.25 bits (308), Expect = 3.205e-85
Identity = 56/56 (100.00%), Postives = 56/56 (100.00%), Query Frame = 3 
Query:  339 FFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 506
            FFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN
Sbjct:   98 FFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 153
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Planmine SMEST
Match: SMESG000073169.1 (SMESG000073169.1)

HSP 1 Score: 152.14 bits (383), Expect = 1.386e-53
Identity = 67/99 (67.68%), Postives = 82/99 (82.83%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            MEF+VA+    KF VE+Q E+Q+GLGELPF KM+KS  GVC  ++ N C +G RCPLRHIKG RT+VCKH+L GLCKKGD+CE+LHEY+MNKMPECYF+
Sbjct:    1 MEFLVADVHNYKFIVEAQFEEQIGLGELPFIKMDKSRAGVCQLFLENNCILGDRCPLRHIKGHRTIVCKHYLNGLCKKGDECEYLHEYNMNKMPECYFY 99

HSP 2 Score: 75.0998 bits (183), Expect = 1.386e-53
Identity = 34/56 (60.71%), Postives = 43/56 (76.79%), Query Frame = 3 
Query:  339 FFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 506
            F+SK+GECQN EC + H     +I++C WYDRGFCRNGP+C   H R++ACKNYIN
Sbjct:   98 FYSKFGECQNSECMYTHT--KFQIKNCPWYDRGFCRNGPNCNFTHERKIACKNYIN 151
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Planmine SMEST
Match: SMESG000073172.1 (SMESG000073172.1)

HSP 1 Score: 112.464 bits (280), Expect = 8.423e-40
Identity = 50/99 (50.51%), Postives = 65/99 (65.66%), Query Frame = 2 
Query:   47 MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFF 343
            M+ +VA+ S   F +   LE Q+G+  LPF KM+K    VC++Y+   C +   CP RH+      VCKH+L GLCKKGD CEFLH YDM+KMP+CYFF
Sbjct:    1 MDSLVADDSKFHFAINDLLEKQIGVATLPFPKMDKCKAPVCNWYLAGTCELDDLCPHRHMNDRSNTVCKHYLNGLCKKGDSCEFLHMYDMSKMPDCYFF 99

HSP 2 Score: 68.9366 bits (167), Expect = 8.423e-40
Identity = 33/63 (52.38%), Postives = 42/63 (66.67%), Query Frame = 3 
Query:  318 TKCQNVIFFSKYGECQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 506
            +K  +  FF+KYGECQN+ C + H     +I +C WYDRGFC  GP CK  H  ++ACKNYIN
Sbjct:   91 SKMPDCYFFTKYGECQNESCAYTHN--RVEIPNCPWYDRGFCNYGPACKLLHEWKIACKNYIN 151
[Close]
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Planmine SMEST
Match: SMESG000074889.1 (SMESG000074889.1)

HSP 1 Score: 100.138 bits (248), Expect = 6.982e-30
Identity = 44/88 (50.00%), Postives = 60/88 (68.18%), Query Frame = 2 
Query:   77 IKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICTMGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYF 340
             +F +ES ++ +VGL  LPF KM+KS   +C  +++  C +G  CP RH+  +   VCKH+L GLCKKGD CEFLH YDM+KM +C F
Sbjct:    9 FEFNIESLIDKKVGLSTLPFLKMDKSKAPICFGFLSGNCDLGNFCPHRHVNNNANTVCKHYLNGLCKKGDACEFLHAYDMSKMADCQF 96

HSP 2 Score: 47.7506 bits (112), Expect = 6.982e-30
Identity = 21/49 (42.86%), Postives = 32/49 (65.31%), Query Frame = 3 
Query:  360 CQNKECPFLHIDPSTKIQDCQWYDRGFCRNGPHCKNRHVRRVACKNYIN 506
            C N++C + H     +IQ C WYDRGFC+ G  C  +HV+++ C N++N
Sbjct:  103 CTNEDCIYNH--RKEEIQACPWYDRGFCKLGNTCNLKHVQKIMCNNFLN 149
The following BLAST results are available for this feature:
BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CPSF48.377e-5560.61cleavage and polyadenylation specific factor 4 [So... [more]
CPSF41.812e-5460.61cleavage and polyadenylation specific factor 4 [So... [more]
CPSF4L2.768e-4651.52cleavage and polyadenylation specific factor 4 lik... [more]
CPSF44.328e-4060.61cleavage and polyadenylation specific factor 4 [So... [more]
CPSF4L4.675e-2955.56cleavage and polyadenylation specific factor 4 lik... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
cpsf-45.203e-5052.88Cleavage and Polyadenylation Specificity Factor [... [more]
cpsf-45.203e-5052.88Cleavage and Polyadenylation Specificity Factor [... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Clp1.997e-5261.39gene:FBgn0015621 transcript:FBtr0078011[more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
cpsf41.798e-5459.60cleavage and polyadenylation specific factor 4 [So... [more]
zc3h38.941e-1342.59zinc finger CCCH-type containing 3 [Source:ZFIN;Ac... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ubxn79.913e-5458.59UBX domain protein 7 [Source:Xenbase;Acc:XB-GENE-5... [more]
ubxn71.009e-3858.59UBX domain protein 7 [Source:Xenbase;Acc:XB-GENE-5... [more]
cpsf4l5.475e-3850.42cleavage and polyadenylation specific factor 4-lik... [more]
ubxn77.318e-3852.14UBX domain protein 7 [Source:Xenbase;Acc:XB-GENE-5... [more]
cpsf4l1.981e-2642.86cleavage and polyadenylation specific factor 4-lik... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Cpsf47.350e-5560.61cleavage and polyadenylation specific factor 4 [So... [more]
Cpsf41.464e-5460.61cleavage and polyadenylation specific factor 4 [So... [more]
Cpsf41.476e-5460.61cleavage and polyadenylation specific factor 4 [So... [more]
Cpsf42.121e-4669.70cleavage and polyadenylation specific factor 4 [So... [more]
Cpsf4l1.056e-4253.06cleavage and polyadenylation specific factor 4-lik... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O95639|CPSF4_HUMAN3.316e-5460.61Cleavage and polyadenylation specificity factor su... [more]
sp|O19137|CPSF4_BOVIN7.175e-5460.61Cleavage and polyadenylation specificity factor su... [more]
sp|Q5FVR7|CPSF4_RAT7.794e-5460.61Cleavage and polyadenylation specificity factor su... [more]
sp|Q6DJP7|CPSF4_XENLA5.234e-5357.58Cleavage and polyadenylation specificity factor su... [more]
sp|Q9VPT8|CPSF4_DROME1.344e-5161.39Cleavage and polyadenylation specificity factor su... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G4VRQ24.167e-6472.73Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A183Q9N74.167e-6472.73Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A419PCI06.910e-6471.72Cleavage and polyadenylation specificity factor su... [more]
A0A074Z8G29.315e-6471.72Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A095B3Z99.472e-6472.73Cleavage and polyadenylation specificity factor su... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cpsf44.706e-5560.61cleavage and polyadenylation specific factor 4 [So... [more]
cpsf45.690e-5560.61cleavage and polyadenylation specific factor 4 [So... [more]
cpsf41.550e-4060.61cleavage and polyadenylation specific factor 4 [So... [more]
zc3h34.570e-1340.74zinc finger CCCH-type containing 3 [Source:ZFIN;Ac... [more]
zc3h34.604e-1340.74zinc finger CCCH-type containing 3 [Source:ZFIN;Ac... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
YTH14.412e-2637.14Essential RNA-binding component of cleavage and po... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO400743.625e-5357.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO339243.867e-640.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cpsf41.250e-5561.62cleavage and polyadenylation specific factor 4 [So... [more]
cpsf42.747e-5561.62cleavage and polyadenylation specific factor 4 [So... [more]
zc3h34.421e-1238.89zinc finger CCCH-type containing 3 [Source:NCBI ge... [more]
zc3h34.606e-1238.89zinc finger CCCH-type containing 3 [Source:NCBI ge... [more]
zc3h61.798e-631.34zinc finger CCCH-type containing 6 [Source:NCBI ge... [more]
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BLAST of Cleavage and polyadenylation specificity factor subunit 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000073125.13.205e-85100.00SMESG000073125.1[more]
SMESG000073169.11.386e-5367.68SMESG000073169.1[more]
SMESG000073172.18.423e-4050.51SMESG000073172.1[more]
SMESG000074889.16.982e-3050.00SMESG000074889.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035837 ID=SMED30035837|Name=Cleavage and polyadenylation specificity factor subunit 4|organism=Schmidtea mediterranea sexual|type=transcript|length=508bp
TAAAATTATAACTGATATAACTAGTTGTTAATTTTGGTTAAATAAAATGG
AATTTATAGTTGCTAATGCAAGTGGAATCAAATTCACAGTTGAAAGTCAA
CTGGAGGATCAAGTTGGTCTTGGTGAATTGCCATTTTCAAAAATGAACAA
GTCTGGTGTTGGAGTTTGTCATTTTTATATGAATAATATTTGTACAATGG
GTCAGCGTTGTCCACTGAGACACATAAAGGGTGATAGGACAGTTGTTTGT
AAGCATTGGTTGAGAGGCTTATGTAAAAAGGGAGATGATTGTGAATTTTT
ACACGAATATGATATGAACAAAATGCCAGAATGTTATTTTTTTTTCCAAA
TATGGCGAATGCCAGAATAAGGAATGCCCTTTTCTGCATATCGACCCGTC
AACTAAAATACAAGATTGTCAGTGGTACGATAGAGGATTTTGCAGAAATG
GTCCACACTGCAAAAATAGACATGTCAGACGGGTGGCTTGTAAAAATTAT
ATCAATGG
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protein sequence of SMED30035837-orf-1

>SMED30035837-orf-1 ID=SMED30035837-orf-1|Name=SMED30035837-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=108bp
MEFIVANASGIKFTVESQLEDQVGLGELPFSKMNKSGVGVCHFYMNNICT
MGQRCPLRHIKGDRTVVCKHWLRGLCKKGDDCEFLHEYDMNKMPECYFFF
QIWRMPE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036855Znf_CCCH_sf
IPR000571Znf_CCCH
Vocabulary: molecular function
TermDefinition
GO:0046872metal ion binding
GO:0003676nucleic acid binding
GO:0008270zinc ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000571Zinc finger, CCCH-typeSMARTSM00356c3hfinal6coord: 35..61
e-value: 1.2
score: 15.8
coord: 62..88
e-value: 1.8E-7
score: 40.8
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 62..89
score: 15.751
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 90..107
score: 8.174
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 35..59
score: 9.0
NoneNo IPR availablePFAMPF18345zf_CCCH_4coord: 68..86
e-value: 2.7E-7
score: 30.4
NoneNo IPR availableGENE3DG3DSA:4.10.1000.10coord: 58..107
e-value: 1.2E-21
score: 78.3
NoneNo IPR availablePANTHERPTHR23102ZINC FINGER CLIPPER -RELATEDcoord: 2..107
IPR036855Zinc finger, CCCH-type superfamilySUPERFAMILYSSF90229CCCH zinc fingercoord: 64..87