40S ribosomal protein S13

Overview
Name40S ribosomal protein S13
Smed IDSMED30035816
Length (bp)593
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 40S ribosomal protein S13 (SMED30035816) t-SNE clustered cells

Violin plots show distribution of expression levels for 40S ribosomal protein S13 (SMED30035816) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 40S ribosomal protein S13 (SMED30035816) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 40S ribosomal protein S13 (SMED30035816) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30035816

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30035816SMESG000042470.1 SMESG000042447.1 SmedASXL_018668SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v4_360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v4_360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v4_360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v4_360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v4_360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v4_360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v4_360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v4_360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v4_360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v4_360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30035816SMESG000042470.1 SMESG000042447.1 dd_Smed_v6_360_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 40S ribosomal protein S13 vs. Ensembl Human
Match: RPS13 (ribosomal protein S13 [Source:HGNC Symbol;Acc:HGNC:10386])

HSP 1 Score: 228.024 bits (580), Expect = 1.310e-76
Identity = 107/151 (70.86%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRIHRLARYYKT+ VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Human
Match: RPS13 (ribosomal protein S13 [Source:HGNC Symbol;Acc:HGNC:10386])

HSP 1 Score: 154.066 bits (388), Expect = 1.756e-47
Identity = 72/107 (67.29%), Postives = 89/107 (83.18%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPK 412
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN K
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRK 107          
BLAST of 40S ribosomal protein S13 vs. Ensembl Human
Match: RPS13 (ribosomal protein S13 [Source:HGNC Symbol;Acc:HGNC:10386])

HSP 1 Score: 92.0485 bits (227), Expect = 1.052e-23
Identity = 50/116 (43.10%), Postives = 64/116 (55.17%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPS-----------------------------------KIGVFLRDTLGVASTKTFATSTVLRILKAKGLA 334
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE IYKLAKKGL PS                                   +IGV LRD+ GVA  +    + +LRILK+KGLA
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIVLLLSPSLECSGAISAHCNLHLLDSSDSPASASRIGVILRDSHGVAQVRFVTGNKILRILKSKGLA 116          
BLAST of 40S ribosomal protein S13 vs. Ensembl Celegans
Match: rps-13 (40S ribosomal protein S13 [Source:UniProtKB/Swiss-Prot;Acc:P51404])

HSP 1 Score: 211.846 bits (538), Expect = 9.608e-71
Identity = 96/151 (63.58%), Postives = 126/151 (83.44%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG+++SA PY+RS+P W K+ AEE+++ I K+AKKGL+PS+IGV LRD+ GV   +  A + + RILK+KG+APE+PEDLY+L+KKAVA+RKHLER+ KD D K+RLILVESRIHRLARYYKT+  L P+WKYE+ TA++LVS
Sbjct:    1 MGRMHNPGKGMAKSAIPYRRSVPSWQKMTAEEVQDQIVKMAKKGLRPSQIGVILRDSHGVGQVRRLAGNKIFRILKSKGMAPELPEDLYHLVKKAVAIRKHLERSRKDIDSKYRLILVESRIHRLARYYKTKRQLPPTWKYESGTAASLVS 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Fly
Match: RpS13 (gene:FBgn0010265 transcript:FBtr0100541)

HSP 1 Score: 221.861 bits (564), Expect = 1.545e-74
Identity = 104/151 (68.87%), Postives = 127/151 (84.11%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKGISQSA PY+R++P W KLNA+++KE I KL KKGL PSKIG+ LRD+ GVA  +    + +LRI+K+ GL P+IPEDLY++IKKAVA+RKHLERN KDKD KFRLILVESRIHRLARYYKT+ VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGISQSALPYRRTVPSWLKLNADDVKEQIKKLGKKGLTPSKIGIILRDSHGVAQVRFVNGNKILRIMKSVGLKPDIPEDLYHMIKKAVAIRKHLERNRKDKDGKFRLILVESRIHRLARYYKTKSVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Fly
Match: RpS13 (gene:FBgn0010265 transcript:FBtr0346454)

HSP 1 Score: 221.861 bits (564), Expect = 1.545e-74
Identity = 104/151 (68.87%), Postives = 127/151 (84.11%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKGISQSA PY+R++P W KLNA+++KE I KL KKGL PSKIG+ LRD+ GVA  +    + +LRI+K+ GL P+IPEDLY++IKKAVA+RKHLERN KDKD KFRLILVESRIHRLARYYKT+ VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGISQSALPYRRTVPSWLKLNADDVKEQIKKLGKKGLTPSKIGIILRDSHGVAQVRFVNGNKILRIMKSVGLKPDIPEDLYHMIKKAVAIRKHLERNRKDKDGKFRLILVESRIHRLARYYKTKSVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Fly
Match: RpS13 (gene:FBgn0010265 transcript:FBtr0079724)

HSP 1 Score: 221.861 bits (564), Expect = 1.545e-74
Identity = 104/151 (68.87%), Postives = 127/151 (84.11%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKGISQSA PY+R++P W KLNA+++KE I KL KKGL PSKIG+ LRD+ GVA  +    + +LRI+K+ GL P+IPEDLY++IKKAVA+RKHLERN KDKD KFRLILVESRIHRLARYYKT+ VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGISQSALPYRRTVPSWLKLNADDVKEQIKKLGKKGLTPSKIGIILRDSHGVAQVRFVNGNKILRIMKSVGLKPDIPEDLYHMIKKAVAIRKHLERNRKDKDGKFRLILVESRIHRLARYYKTKSVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Zebrafish
Match: rps13 (ribosomal protein S13 [Source:ZFIN;Acc:ZDB-GENE-040625-52])

HSP 1 Score: 229.18 bits (583), Expect = 2.419e-77
Identity = 108/151 (71.52%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE I+KLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLILVESRIHRLARYYKTR VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIFKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILVESRIHRLARYYKTRRVLAPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Zebrafish
Match: rps13 (ribosomal protein S13 [Source:ZFIN;Acc:ZDB-GENE-040625-52])

HSP 1 Score: 229.18 bits (583), Expect = 2.419e-77
Identity = 108/151 (71.52%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE I+KLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLILVESRIHRLARYYKTR VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIFKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILVESRIHRLARYYKTRRVLAPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Zebrafish
Match: rps13 (ribosomal protein S13 [Source:ZFIN;Acc:ZDB-GENE-040625-52])

HSP 1 Score: 154.066 bits (388), Expect = 4.101e-48
Identity = 74/122 (60.66%), Postives = 95/122 (77.87%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRI 457
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE I+KLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN     +KF L    +R+
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIFKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERN-----RKFVLCFHRTRM 117          
BLAST of 40S ribosomal protein S13 vs. Ensembl Xenopus
Match: tm4sf1 (transmembrane 4 L six family member 1 [Source:Xenbase;Acc:XB-GENE-853117])

HSP 1 Score: 195.282 bits (495), Expect = 7.929e-64
Identity = 97/147 (65.99%), Postives = 117/147 (79.59%), Query Frame = 2
Query:  104 HSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            H  G  +S S +P     P W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRI RLARYYKTR VL P+WKYE++TASALV+
Sbjct:    5 HRMGLTLSCSFSP-----PSWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIPRLARYYKTRRVLPPNWKYESSTASALVA 146          
BLAST of 40S ribosomal protein S13 vs. Ensembl Xenopus
Match: tm4sf1 (transmembrane 4 L six family member 1 [Source:Xenbase;Acc:XB-GENE-853117])

HSP 1 Score: 188.734 bits (478), Expect = 1.843e-61
Identity = 91/127 (71.65%), Postives = 109/127 (85.83%), Query Frame = 2
Query:  164 WFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRI RLARYYKTR VL P+WKYE++TASALV+
Sbjct:   10 WLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIPRLARYYKTRRVLPPNWKYESSTASALVA 136          
BLAST of 40S ribosomal protein S13 vs. Ensembl Xenopus
Match: tm4sf1 (transmembrane 4 L six family member 1 [Source:Xenbase;Acc:XB-GENE-853117])

HSP 1 Score: 173.711 bits (439), Expect = 2.748e-55
Identity = 84/117 (71.79%), Postives = 99/117 (84.62%), Query Frame = 2
Query:  161 GWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWK 511
            GW KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRI RLARYYKTR VL P+WK
Sbjct:   32 GWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIPRLARYYKTRRVLPPNWK 148          
BLAST of 40S ribosomal protein S13 vs. Ensembl Xenopus
Match: p2ry6 (pyrimidinergic receptor P2Y, G-protein coupled, 6 [Source:Xenbase;Acc:XB-GENE-955049])

HSP 1 Score: 49.2914 bits (116), Expect = 5.349e-8
Identity = 24/47 (51.06%), Postives = 31/47 (65.96%), Query Frame = 2
Query:  101 MHSSGKGISQSATPYKRSIPGWFK---LNAEEIKENIYKLAKKGLQP 232
            M++  KG+SQSA PY+RS P W K   L ++ + E IYKLA K L P
Sbjct:    4 MYAPRKGLSQSALPYRRSGPTWLKGVTLTSDNVNEQIYKLAMKDLTP 50          
BLAST of 40S ribosomal protein S13 vs. Ensembl Mouse
Match: Rps13 (ribosomal protein S13 [Source:MGI Symbol;Acc:MGI:1915302])

HSP 1 Score: 228.024 bits (580), Expect = 8.995e-77
Identity = 107/151 (70.86%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRIHRLARYYKT+ VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Mouse
Match: Rps13 (ribosomal protein S13 [Source:MGI Symbol;Acc:MGI:1915302])

HSP 1 Score: 209.92 bits (533), Expect = 7.994e-70
Identity = 99/140 (70.71%), Postives = 119/140 (85.00%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWK 511
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRIHRLARYYKT+ VL P+WK
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWK 140          
BLAST of 40S ribosomal protein S13 vs. Ensembl Mouse
Match: Rps13 (ribosomal protein S13 [Source:MGI Symbol;Acc:MGI:1915302])

HSP 1 Score: 79.7221 bits (195), Expect = 8.073e-20
Identity = 34/51 (66.67%), Postives = 43/51 (84.31%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIG 244
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE IYKLAKKGL PS+IG
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIG 51          
BLAST of 40S ribosomal protein S13 vs. UniProt/SwissProt
Match: sp|O77303|RS13_LUMRU (40S ribosomal protein S13 OS=Lumbricus rubellus OX=35632 GN=RPS13 PE=2 SV=3)

HSP 1 Score: 232.646 bits (592), Expect = 6.990e-78
Identity = 114/151 (75.50%), Postives = 128/151 (84.77%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKGISQSA PY+RS+P W KL  +++KE IYKLAKKGL PS+IGV LRD+ GVA T+  A + +LRILKAKGLAP IPEDLY LIKKAVA+RKHLERN KDKD KFRLILVESRIHRLARYYKTR VL P WKYE+ATASALV+
Sbjct:    1 MGRMHAPGKGISQSALPYRRSVPTWLKLGPDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQTRHVAGNKILRILKAKGLAPTIPEDLYFLIKKAVAIRKHLERNRKDKDSKFRLILVESRIHRLARYYKTRKVLPPVWKYESATASALVA 151          
BLAST of 40S ribosomal protein S13 vs. UniProt/SwissProt
Match: sp|P49393|RS13_XENLA (40S ribosomal protein S13 OS=Xenopus laevis OX=8355 GN=rps13 PE=3 SV=2)

HSP 1 Score: 229.18 bits (583), Expect = 1.950e-76
Identity = 108/151 (71.52%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRIHRLARYYKTR VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTRRVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. UniProt/SwissProt
Match: sp|P62278|RS13_RAT (40S ribosomal protein S13 OS=Rattus norvegicus OX=10116 GN=Rps13 PE=1 SV=2)

HSP 1 Score: 228.024 bits (580), Expect = 6.292e-76
Identity = 107/151 (70.86%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRIHRLARYYKT+ VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. UniProt/SwissProt
Match: sp|P62301|RS13_MOUSE (40S ribosomal protein S13 OS=Mus musculus OX=10090 GN=Rps13 PE=1 SV=2)

HSP 1 Score: 228.024 bits (580), Expect = 6.292e-76
Identity = 107/151 (70.86%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRIHRLARYYKT+ VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. UniProt/SwissProt
Match: sp|P62277|RS13_HUMAN (40S ribosomal protein S13 OS=Homo sapiens OX=9606 GN=RPS13 PE=1 SV=2)

HSP 1 Score: 228.024 bits (580), Expect = 6.292e-76
Identity = 107/151 (70.86%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG+SQSA PY+RS+P W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRIHRLARYYKT+ VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. TrEMBL
Match: T1FX82 (Ribosomal_S13_N domain-containing protein OS=Helobdella robusta OX=6412 GN=20213430 PE=3 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 3.082e-76
Identity = 112/151 (74.17%), Postives = 132/151 (87.42%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKGISQS+ PY+RS+P W KL+++++KE IYKLAKKGL PS+IGV LRD+ GVA  K+  ++ +LRILKAKGLAPEIPEDLY LIKKAVA+RKHLERN KDKD KFRLILVESRIHRLARYYKT+ VL P+WKYE++TASALVS
Sbjct:    1 MGRMHAPGKGISQSSLPYRRSVPTWLKLSSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVKSVTSNKILRILKAKGLAPEIPEDLYFLIKKAVAIRKHLERNRKDKDAKFRLILVESRIHRLARYYKTKKVLPPTWKYESSTASALVS 151          
BLAST of 40S ribosomal protein S13 vs. TrEMBL
Match: A0A1S3JW81 (40S ribosomal protein S13 OS=Lingula unguis OX=7574 GN=LOC106176642 PE=3 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 2.754e-75
Identity = 111/151 (73.51%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            MVRMH+ GKGISQSA PY+RSIP W KL ++++KE IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILKAKGLAP+IPEDLY+LIKKAVA+RKHLERN KD D KFRLILVESRIHRLARYYK++ VL P+WKYE+ATASALV+
Sbjct:    1 MVRMHAPGKGISQSALPYRRSIPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKAKGLAPDIPEDLYHLIKKAVAIRKHLERNRKDTDSKFRLILVESRIHRLARYYKSKKVLPPNWKYESATASALVA 151          
BLAST of 40S ribosomal protein S13 vs. TrEMBL
Match: A8UAD8 (Putative ribosomal protein S13 OS=Barentsia elongata OX=478378 PE=2 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 8.986e-75
Identity = 110/151 (72.85%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKGISQSA PY+RS+  W KL+++++KE I+KLAKKGL PS+IGV LRD+ GVA  +    + +LRILKAKGLAPEIPEDLY+LIKKAV++RKHLERN KDKD KFRLILVESRIHRLARYYKTR VL P+WKYE+ATASALV+
Sbjct:    1 MGRMHAPGKGISQSALPYRRSVATWLKLSSDDVKEQIFKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKAKGLAPEIPEDLYHLIKKAVSIRKHLERNRKDKDSKFRLILVESRIHRLARYYKTRQVLAPNWKYESATASALVA 151          
BLAST of 40S ribosomal protein S13 vs. TrEMBL
Match: A0A0L8G2L9 (Ribosomal_S13_N domain-containing protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22001297mg PE=3 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 2.599e-74
Identity = 109/151 (72.19%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH  GKGISQSA PY+RS+P W KL+++++KE IYKLAKKGL PSKIGV LRD+ GVA  +    + +LRILKAKGLAP+IPEDLY+LIKKAV+VRKH+ERN KDKD KFRLIL+ESRIHRLARYYKT+ VL P+WKYE++TASALV+
Sbjct:    1 MGRMHCPGKGISQSALPYRRSVPTWLKLSSDDVKEQIYKLAKKGLTPSKIGVILRDSHGVAQVRFVTGNKILRILKAKGLAPKIPEDLYHLIKKAVSVRKHMERNRKDKDSKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. TrEMBL
Match: A0A2R5LGI4 (Putative 40s ribosomal protein s13 OS=Ornithodoros turicata OX=34597 PE=3 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 2.900e-74
Identity = 108/151 (71.52%), Postives = 128/151 (84.77%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH  GKGISQSA PY+RS+P W KL A+++KE+IYKLAKKGL PS+IGV LRD+ GVA  +    + +LRILKAKGLAP++PEDLY LIKKAVA+RKHLERN KD+D KFRLILVESRIHRL RYYKT+ VL PSW+YE++TASALV+
Sbjct:    1 MGRMHCGGKGISQSALPYRRSVPTWLKLTADDVKEHIYKLAKKGLTPSQIGVILRDSHGVAQVRWVTGNKILRILKAKGLAPQLPEDLYCLIKKAVAIRKHLERNRKDRDSKFRLILVESRIHRLTRYYKTKRVLAPSWRYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Cavefish
Match: rps13 (ribosomal protein S13 [Source:NCBI gene;Acc:103021988])

HSP 1 Score: 226.868 bits (577), Expect = 2.020e-76
Identity = 106/151 (70.20%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG++QSA PY+RS+P W KL ++++KE I+KLAKKGL PS+IGV LRD+ GVA  +  A + +LRILK+KGLAP++PEDLY+LIKKAVA RKHLERN KDKD KFRLILVESRIHRLARYYK+R VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLAQSALPYRRSVPTWLKLTSDDVKEQIFKLAKKGLTPSQIGVILRDSHGVAQVRFVAGNKILRILKSKGLAPDLPEDLYHLIKKAVATRKHLERNRKDKDAKFRLILVESRIHRLARYYKSRRVLAPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Cavefish
Match: rps13 (ribosomal protein S13 [Source:NCBI gene;Acc:103021988])

HSP 1 Score: 226.868 bits (577), Expect = 2.020e-76
Identity = 106/151 (70.20%), Postives = 130/151 (86.09%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG++QSA PY+RS+P W KL ++++KE I+KLAKKGL PS+IGV LRD+ GVA  +  A + +LRILK+KGLAP++PEDLY+LIKKAVA RKHLERN KDKD KFRLILVESRIHRLARYYK+R VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLAQSALPYRRSVPTWLKLTSDDVKEQIFKLAKKGLTPSQIGVILRDSHGVAQVRFVAGNKILRILKSKGLAPDLPEDLYHLIKKAVATRKHLERNRKDKDAKFRLILVESRIHRLARYYKSRRVLAPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002163.1 (pep scaffold:Pmarinus_7.0:GL483698:6396:13204:-1 gene:ENSPMAG00000001957.1 transcript:ENSPMAT00000002163.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 196.823 bits (499), Expect = 2.487e-65
Identity = 95/153 (62.09%), Postives = 122/153 (79.74%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPG--WFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKG+SQSA PY+RS+P   W KL ++++KE I+KLAKKGL PS+IGV LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLER+ K++    R   +ESRIHRLARYY  + VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGLSQSALPYRRSVPTQQWLKLTSDDVKEQIFKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERSRKEESMAPRRCFLESRIHRLARYYNFKRVLPPNWKYESSTASALVA 153          
BLAST of 40S ribosomal protein S13 vs. Ensembl Yeast
Match: RPS13 (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 [Source:SGD;Acc:S000002471])

HSP 1 Score: 214.927 bits (546), Expect = 1.035e-72
Identity = 103/151 (68.21%), Postives = 121/151 (80.13%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMHS+GKGIS SA PY R+ P WFKL++E + E I K A+KGL PS+IGV LRD  GV   +    + ++RILK+ GLAPEIPEDLY LIKKAV+VRKHLERN KDKD KFRLIL+ESRIHRLARYY+T  VL P+WKYE+ATASALV+
Sbjct:    1 MGRMHSAGKGISSSAIPYSRNAPAWFKLSSESVIEQIVKYARKGLTPSQIGVLLRDAHGVTQARVITGNKIMRILKSNGLAPEIPEDLYYLIKKAVSVRKHLERNRKDKDAKFRLILIESRIHRLARYYRTVAVLPPNWKYESATASALVN 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Nematostella
Match: EDO33960 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQP1])

HSP 1 Score: 221.861 bits (564), Expect = 6.780e-75
Identity = 105/151 (69.54%), Postives = 127/151 (84.11%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+ GKGIS SA PY+RS+P W KL ++++KE +YKLAKKGL PS+IGV LRD+ GVA  +    + +LRILKAKGLAP +PEDLY LIKKAVAVRKHLE+N KDKD KFRLIL+ESRIHRLARY+KT+ VL P+WKYE++TASALV+
Sbjct:    1 MGRMHAPGKGISGSALPYRRSVPTWQKLTSDDVKEQMYKLAKKGLTPSQIGVILRDSYGVAQVRYITGNKILRILKAKGLAPSLPEDLYCLIKKAVAVRKHLEKNRKDKDSKFRLILIESRIHRLARYFKTKRVLPPNWKYESSTASALVA 151          
BLAST of 40S ribosomal protein S13 vs. Ensembl Medaka
Match: rps13 (ribosomal protein S13 [Source:NCBI gene;Acc:101154861])

HSP 1 Score: 205.297 bits (521), Expect = 5.182e-68
Identity = 103/151 (68.21%), Postives = 127/151 (84.11%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            M RMH+  KG+SQSA PY+RS+P W KL ++++KE I+KLAKKGL PS+I V LRD+ GVA  +    + +LRILK+KGLAP++PEDLY+LIKKAVAVRKHLERN KDKD KFRLIL+ESRIHRLARYYKT+ VL P+WK E++TASALV+
Sbjct:    1 MGRMHAP-KGLSQSALPYRRSVPTWLKLTSDDVKEQIFKLAKKGLTPSQI-VILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLAPNWK-ESSTASALVA 148          
BLAST of 40S ribosomal protein S13 vs. Planmine SMEST
Match: SMESG000042447.1 (SMESG000042447.1)

HSP 1 Score: 307.76 bits (787), Expect = 1.378e-108
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 2
Query:   92 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS
Sbjct:    1 MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 151          
BLAST of 40S ribosomal protein S13 vs. Planmine SMEST
Match: SMESG000042447.1 (SMESG000042447.1)

HSP 1 Score: 301.597 bits (771), Expect = 3.645e-106
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 2
Query:  101 MHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 544
            MHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS
Sbjct:    1 MHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKIGVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRKHLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALVS 148          
The following BLAST results are available for this feature:
BLAST of 40S ribosomal protein S13 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
RPS131.310e-7670.86ribosomal protein S13 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS131.756e-4767.29ribosomal protein S13 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS131.052e-2343.10ribosomal protein S13 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of 40S ribosomal protein S13 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps-139.608e-7163.5840S ribosomal protein S13 [Source:UniProtKB/Swiss... [more]
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BLAST of 40S ribosomal protein S13 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
RpS131.545e-7468.87gene:FBgn0010265 transcript:FBtr0100541[more]
RpS131.545e-7468.87gene:FBgn0010265 transcript:FBtr0346454[more]
RpS131.545e-7468.87gene:FBgn0010265 transcript:FBtr0079724[more]
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BLAST of 40S ribosomal protein S13 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rps132.419e-7771.52ribosomal protein S13 [Source:ZFIN;Acc:ZDB-GENE-04... [more]
rps132.419e-7771.52ribosomal protein S13 [Source:ZFIN;Acc:ZDB-GENE-04... [more]
rps134.101e-4860.66ribosomal protein S13 [Source:ZFIN;Acc:ZDB-GENE-04... [more]
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BLAST of 40S ribosomal protein S13 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
tm4sf17.929e-6465.99transmembrane 4 L six family member 1 [Source:Xenb... [more]
tm4sf11.843e-6171.65transmembrane 4 L six family member 1 [Source:Xenb... [more]
tm4sf12.748e-5571.79transmembrane 4 L six family member 1 [Source:Xenb... [more]
p2ry65.349e-851.06pyrimidinergic receptor P2Y, G-protein coupled, 6 ... [more]
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BLAST of 40S ribosomal protein S13 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Rps138.995e-7770.86ribosomal protein S13 [Source:MGI Symbol;Acc:MGI:1... [more]
Rps137.994e-7070.71ribosomal protein S13 [Source:MGI Symbol;Acc:MGI:1... [more]
Rps138.073e-2066.67ribosomal protein S13 [Source:MGI Symbol;Acc:MGI:1... [more]
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BLAST of 40S ribosomal protein S13 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O77303|RS13_LUMRU6.990e-7875.5040S ribosomal protein S13 OS=Lumbricus rubellus OX... [more]
sp|P49393|RS13_XENLA1.950e-7671.5240S ribosomal protein S13 OS=Xenopus laevis OX=835... [more]
sp|P62278|RS13_RAT6.292e-7670.8640S ribosomal protein S13 OS=Rattus norvegicus OX=... [more]
sp|P62301|RS13_MOUSE6.292e-7670.8640S ribosomal protein S13 OS=Mus musculus OX=10090... [more]
sp|P62277|RS13_HUMAN6.292e-7670.8640S ribosomal protein S13 OS=Homo sapiens OX=9606 ... [more]
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BLAST of 40S ribosomal protein S13 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1FX823.082e-7674.17Ribosomal_S13_N domain-containing protein OS=Helob... [more]
A0A1S3JW812.754e-7573.5140S ribosomal protein S13 OS=Lingula unguis OX=757... [more]
A8UAD88.986e-7572.85Putative ribosomal protein S13 OS=Barentsia elonga... [more]
A0A0L8G2L92.599e-7472.19Ribosomal_S13_N domain-containing protein OS=Octop... [more]
A0A2R5LGI42.900e-7471.52Putative 40s ribosomal protein s13 OS=Ornithodoros... [more]
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BLAST of 40S ribosomal protein S13 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps132.020e-7670.20ribosomal protein S13 [Source:NCBI gene;Acc:103021... [more]
rps132.020e-7670.20ribosomal protein S13 [Source:NCBI gene;Acc:103021... [more]
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BLAST of 40S ribosomal protein S13 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000002163.12.487e-6562.09pep scaffold:Pmarinus_7.0:GL483698:6396:13204:-1 g... [more]
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BLAST of 40S ribosomal protein S13 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
RPS131.035e-7268.21Protein component of the small (40S) ribosomal sub... [more]
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BLAST of 40S ribosomal protein S13 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO339606.780e-7569.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 40S ribosomal protein S13 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps135.182e-6868.21ribosomal protein S13 [Source:NCBI gene;Acc:101154... [more]
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BLAST of 40S ribosomal protein S13 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000042447.11.378e-108100.00SMESG000042447.1[more]
SMESG000042447.13.645e-106100.00SMESG000042447.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035816 ID=SMED30035816|Name=40S ribosomal protein S13|organism=Schmidtea mediterranea sexual|type=transcript|length=593bp
TTTATTTTAATTATAAACATCTTATATAAAATATATTTGTTTAAATTTTC
TTGTTAAAGTTTATACTAAAAATTTAAATTATAAATTAATCATGGTTCGT
ATGCATTCATCTGGAAAGGGTATATCCCAAAGTGCAACTCCTTACAAACG
ATCGATTCCTGGATGGTTTAAGTTAAATGCTGAAGAAATTAAAGAAAATA
TATATAAATTAGCCAAAAAAGGCCTACAACCTTCAAAAATTGGTGTATTT
TTGAGAGATACATTAGGTGTTGCAAGCACTAAAACCTTTGCAACTAGTAC
GGTCTTAAGAATTTTAAAAGCAAAGGGCCTTGCTCCAGAAATTCCAGAAG
ATTTATACAATTTGATCAAAAAAGCTGTTGCTGTAAGAAAACATCTCGAA
AGAAATCCAAAAGACAAAGATCAAAAATTCCGTTTGATCTTAGTTGAAAG
CCGTATTCACAGATTAGCTAGATATTACAAAACTAGACATGTTTTGGAAC
CATCATGGAAATACGAAGCTGCTACGGCATCAGCTTTAGTTTCATAAGAA
ATTTTAAATAAAACTTTGGATTTAACTTTAAAAAAAAAGATCG
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protein sequence of SMED30035816-orf-1

>SMED30035816-orf-1 ID=SMED30035816-orf-1|Name=SMED30035816-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=152bp
MVRMHSSGKGISQSATPYKRSIPGWFKLNAEEIKENIYKLAKKGLQPSKI
GVFLRDTLGVASTKTFATSTVLRILKAKGLAPEIPEDLYNLIKKAVAVRK
HLERNPKDKDQKFRLILVESRIHRLARYYKTRHVLEPSWKYEAATASALV
S*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR023029Ribosomal_S15P
IPR000589Ribosomal_S15
IPR012606Ribosomal_S13/S15_N
IPR009068S15_NS1_RNA-bd
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
GO:0005840ribosome
GO:0030529intracellular ribonucleoprotein complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012606Ribosomal protein S13/S15, N-terminalSMARTSM01386Ribosomal_S13_N_2coord: 1..60
e-value: 2.7E-29
score: 113.3
IPR012606Ribosomal protein S13/S15, N-terminalPFAMPF08069Ribosomal_S13_Ncoord: 1..60
e-value: 1.9E-26
score: 91.9
IPR000589Ribosomal protein S15SMARTSM01387Ribosomal_S15_2coord: 70..149
e-value: 1.8E-10
score: 50.8
IPR000589Ribosomal protein S15PFAMPF00312Ribosomal_S15coord: 75..146
e-value: 3.1E-14
score: 53.0
IPR000589Ribosomal protein S15CDDcd00353Ribosomal_S15p_S13ecoord: 71..142
e-value: 1.36E-22
score: 83.3214
NoneNo IPR availableGENE3DG3DSA:1.10.8.1030coord: 35..82
e-value: 1.1E-19
score: 71.9
NoneNo IPR availableGENE3DG3DSA:1.10.287.10coord: 83..151
e-value: 4.4E-36
score: 124.7
IPR023029Ribosomal protein S15PPANTHERPTHR11885RIBOSOMAL PROTEIN S15P/S13Ecoord: 1..150
IPR009068S15/NS1, RNA-bindingSUPERFAMILYSSF47060S15/NS1 RNA-binding domaincoord: 70..142