Serine/threonine-protein kinase fused

Overview


Name	Serine/threonine-protein kinase fused
Smed ID	SMED30035808
Length (bp)	1308

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Serine/threonine-protein kinase fused (SMED30035808) t-SNE clustered cells

Violin plots show distribution of expression levels for Serine/threonine-protein kinase fused (SMED30035808) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Serine/threonine-protein kinase fused (SMED30035808) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Serine/threonine-protein kinase fused (SMED30035808) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30035808

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 8

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30035808	SMESG000028762.1	Contig4255	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30035808	SMESG000078507.1 SMESG000078509.1	Contig4255	GPL15192	PMID:23079596 Forsthoefel et al., 2012
pharynx	SMED30035808	SMESG000078509.1 SMESG000078507.1	dd_Smed_v4_4259_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30035808	SMESG000078509.1 SMESG000078507.1	dd_Smed_v4_4259_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30035808	SMESG000078509.1 SMESG000078507.1	dd_Smed_v4_4259_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30035808	SMESG000078509.1 SMESG000078507.1	Contig37593	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30035808	SMESG000078509.1 SMESG000078507.1	Contig37593	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
tail region	SMED30035808	SMESG000078509.1 SMESG000078507.1	dd_Smed_v6_4259_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Human
Match: PAK1 (p21 (RAC1) activated kinase 1 [Source:HGNC Symbol;Acc:HGNC:8590])

HSP 1 Score: 83.5741 bits (205), Expect = 1.034e-17
Identity = 55/157 (35.03%), Postives = 82/157 (52.23%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYG-CSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            + E +I  +  E L+ L+ LH  +++H DIK +NILL   G   L D+G C+++   Q+     K+    GT  + APE+    +Y  K DIWSLGI+ IEMIEG+   LN+      Y    N   + +    +S   R+ L +CL  D+  R SA
Sbjct:   83 MDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQS-----KRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSA 234

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Human
Match: STK36 (serine/threonine kinase 36 [Source:HGNC Symbol;Acc:HGNC:17209])

HSP 1 Score: 83.5741 bits (205), Expect = 1.890e-17
Identity = 49/152 (32.24%), Postives = 79/152 (51.97%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKD----KNINFKFRLRF---ISENLRNILMKCLTNDMAARSS 611
            + E+++  +A +++  L  LH  +ILH D+KP+NILL K G   LCD+G ++ +   T ++   K    GT  Y +PE+ +   Y   AD+WS+G ++ E+  G    YA       ++  K  +R+   IS   +N L   LT D   R S
Sbjct:   97 LPEDQVQAIAAQLVSALYYLHSHRILHRDMKPQNILLAKGGGIKLCDFGFARAMSTNTMVLTSIK----GTPLYMSPELVEERPYDHTADLWSVGCILYELAVGTPPFYATSIFQLVSLILKDPVRWPSTISPCFKNFLQGLLTKDPRQRLS 244

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Human
Match: PAK4 (p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;Acc:HGNC:16059])

HSP 1 Score: 85.5001 bits (210), Expect = 2.281e-17
Identity = 49/156 (31.41%), Postives = 83/156 (53.21%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDK---------NINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L VLH   ++H DIK ++ILL   GR  L D+G     C Q +  V ++ +  GT  + APE+     Y  + DIWSLGI++IEM++G+   + +           N+  + + L  +S +L+  L + L  D A R++A
Sbjct:  259 MNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFG----FCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATA 410

[Close]

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDK---------NINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L VLH   ++H DIK ++ILL   GR  L D+G     C Q +  V ++ +  GT  + APE+     Y  + DIWSLGI++IEM++G+   + +           N+  + + L  +S +L+  L + L  D A R++A
Sbjct:  259 MNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFG----FCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATA 410

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Human
Match: PAK4 (p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;Acc:HGNC:16059])

HSP 1 Score: 85.1149 bits (209), Expect = 3.614e-17
Identity = 49/156 (31.41%), Postives = 83/156 (53.21%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDK---------NINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L VLH   ++H DIK ++ILL   GR  L D+G     C Q +  V ++ +  GT  + APE+     Y  + DIWSLGI++IEM++G+   + +           N+  + + L  +S +L+  L + L  D A R++A
Sbjct:  322 MNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFG----FCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATA 473

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Celegans
Match: pak-2 (Serine/threonine-protein kinase pak-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EFU0])

HSP 1 Score: 83.9593 bits (206), Expect = 3.202e-17
Identity = 55/180 (30.56%), Postives = 91/180 (50.56%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKL-----NQYAKDKNINFKFRLRF-----ISENLRNILMKCLTNDMAARSSAXXXXXXXVFKDIQNT*LIKLII*Q 686
            ++E +I  ++ ++L  L  LH  K++H DIK ++ILL + G   L D+G     C Q +  V ++ +  GT  + A E+     Y  +ADIWS GI++IEM+EG+        +   K I  +   RF     +S  L  +L  C+  D+  R  A++LL    F   Q++  I  ++ Q
Sbjct:  322 MTEPQIATISRQVLGALDFLHARKVIHRDIKSDSILLKRDGTVKLTDFG----FCGQLSEEVPRRRSLVGTPYWTAAEVIAREPYDTRADIWSFGIMLIEMVEGEPPFFNDQPFQAMKRIRDEHEARFSRHAKVSVELSELLSHCIVKDVNKRWPAKDLLRHPFFAKAQHSSSIAPLLLQ 497

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Celegans
Match: pak-2 (Serine/threonine-protein kinase pak-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EFU0])

HSP 1 Score: 83.9593 bits (206), Expect = 3.486e-17
Identity = 55/180 (30.56%), Postives = 91/180 (50.56%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKL-----NQYAKDKNINFKFRLRF-----ISENLRNILMKCLTNDMAARSSAXXXXXXXVFKDIQNT*LIKLII*Q 686
            ++E +I  ++ ++L  L  LH  K++H DIK ++ILL + G   L D+G     C Q +  V ++ +  GT  + A E+     Y  +ADIWS GI++IEM+EG+        +   K I  +   RF     +S  L  +L  C+  D+  R  A++LL    F   Q++  I  ++ Q
Sbjct:  320 MTEPQIATISRQVLGALDFLHARKVIHRDIKSDSILLKRDGTVKLTDFG----FCGQLSEEVPRRRSLVGTPYWTAAEVIAREPYDTRADIWSFGIMLIEMVEGEPPFFNDQPFQAMKRIRDEHEARFSRHAKVSVELSELLSHCIVKDVNKRWPAKDLLRHPFFAKAQHSSSIAPLLLQ 495

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Celegans
Match: cst-1 (Serine/threonine-protein kinase cst-1 Serine/threonine-protein kinase cst-1 37kDa subunit Serine/threonine-protein kinase cst-1 18kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:Q9NB31])

HSP 1 Score: 80.4925 bits (197), Expect = 4.565e-16
Identity = 48/109 (44.04%), Postives = 61/109 (55.96%), Query Frame = 3

Query:  165 RVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK 491
            R PL SE EI  +  + LK L+ LH  K +H DIK  NILL+  G A L D+G    +  Q    + K+    GT  + APE+ +   Y  KADIWSLGI  IEM EG+
Sbjct:  123 RKPL-SEQEISAVLRDTLKGLQYLHDLKKIHRDIKAGNILLNTDGIAKLADFG----VAGQLTDTMAKRNTVIGTPFWMAPEVIEEIGYDTKADIWSLGITAIEMAEGR 226

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Celegans
Match: cst-1 (Serine/threonine-protein kinase cst-1 Serine/threonine-protein kinase cst-1 37kDa subunit Serine/threonine-protein kinase cst-1 18kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:Q9NB31])

HSP 1 Score: 80.4925 bits (197), Expect = 4.716e-16
Identity = 48/109 (44.04%), Postives = 61/109 (55.96%), Query Frame = 3

Query:  165 RVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK 491
            R PL SE EI  +  + LK L+ LH  K +H DIK  NILL+  G A L D+G    +  Q    + K+    GT  + APE+ +   Y  KADIWSLGI  IEM EG+
Sbjct:  123 RKPL-SEQEISAVLRDTLKGLQYLHDLKKIHRDIKAGNILLNTDGIAKLADFG----VAGQLTDTMAKRNTVIGTPFWMAPEVIEEIGYDTKADIWSLGITAIEMAEGR 226

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Celegans
Match: cst-2 (Caenorhabditis STE20-like kinase [Source:UniProtKB/TrEMBL;Acc:Q304D8])

HSP 1 Score: 79.7221 bits (195), Expect = 5.491e-16
Identity = 48/109 (44.04%), Postives = 61/109 (55.96%), Query Frame = 3

Query:  165 RVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK 491
            R PL SE EI  +  + LK L+ LH  K +H DIK  NILL+  G A L D+G    +  Q    + K+    GT  + APE+ +   Y  KADIWSLGI  IEM EG+
Sbjct:  123 RKPL-SEQEISAVLRDTLKGLQYLHDLKKIHRDIKAGNILLNTDGIAKLADFG----VAGQLTDTMAKRNTVIGTPFWMAPEVIEEIGYDTKADIWSLGITAIEMAEGR 226

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Fly
Match: Akt1 (gene:FBgn0010379 transcript:FBtr0083226)

HSP 1 Score: 80.1073 bits (196), Expect = 8.876e-16
Identity = 49/152 (32.24%), Postives = 77/152 (50.66%), Query Frame = 3

Query:  174 LISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRL-------RFISENLRNILMKCLTNDMAAR 605
            + +E+       EI+  L  LH   I++ D+K EN+LLDK G   + D+G    +C + +I   +   +F GT +Y APE+  +N Y    D W  G+V+ EMI G+L  Y +D ++ F   L       R I++  +N+L   L  D   R
Sbjct:  279 IFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFG----LCKE-DITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKR 425

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Fly
Match: Akt1 (gene:FBgn0010379 transcript:FBtr0344470)

HSP 1 Score: 80.1073 bits (196), Expect = 8.876e-16
Identity = 49/152 (32.24%), Postives = 77/152 (50.66%), Query Frame = 3

Query:  174 LISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRL-------RFISENLRNILMKCLTNDMAAR 605
            + +E+       EI+  L  LH   I++ D+K EN+LLDK G   + D+G    +C + +I   +   +F GT +Y APE+  +N Y    D W  G+V+ EMI G+L  Y +D ++ F   L       R I++  +N+L   L  D   R
Sbjct:  279 IFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFG----LCKE-DITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKR 425

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Fly
Match: Akt1 (gene:FBgn0010379 transcript:FBtr0344717)

HSP 1 Score: 80.1073 bits (196), Expect = 8.876e-16
Identity = 49/152 (32.24%), Postives = 77/152 (50.66%), Query Frame = 3

Query:  174 LISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRL-------RFISENLRNILMKCLTNDMAAR 605
            + +E+       EI+  L  LH   I++ D+K EN+LLDK G   + D+G    +C + +I   +   +F GT +Y APE+  +N Y    D W  G+V+ EMI G+L  Y +D ++ F   L       R I++  +N+L   L  D   R
Sbjct:  279 IFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFG----LCKE-DITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKR 425

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Fly
Match: Akt1 (gene:FBgn0010379 transcript:FBtr0083227)

HSP 1 Score: 80.1073 bits (196), Expect = 8.876e-16
Identity = 49/152 (32.24%), Postives = 77/152 (50.66%), Query Frame = 3

Query:  174 LISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRL-------RFISENLRNILMKCLTNDMAAR 605
            + +E+       EI+  L  LH   I++ D+K EN+LLDK G   + D+G    +C + +I   +   +F GT +Y APE+  +N Y    D W  G+V+ EMI G+L  Y +D ++ F   L       R I++  +N+L   L  D   R
Sbjct:  279 IFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFG----LCKE-DITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKR 425

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Fly
Match: Akt1 (gene:FBgn0010379 transcript:FBtr0083228)

HSP 1 Score: 79.7221 bits (195), Expect = 1.252e-15
Identity = 49/152 (32.24%), Postives = 77/152 (50.66%), Query Frame = 3

Query:  174 LISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRL-------RFISENLRNILMKCLTNDMAAR 605
            + +E+       EI+  L  LH   I++ D+K EN+LLDK G   + D+G    +C + +I   +   +F GT +Y APE+  +N Y    D W  G+V+ EMI G+L  Y +D ++ F   L       R I++  +N+L   L  D   R
Sbjct:  360 IFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFG----LCKE-DITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKR 506

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Zebrafish
Match: pak7 (p21 protein (Cdc42/Rac)-activated kinase 7 [Source:ZFIN;Acc:ZDB-GENE-040426-2085])

HSP 1 Score: 82.0333 bits (201), Expect = 7.189e-18
Identity = 51/157 (32.48%), Postives = 79/157 (50.32%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +LK L  LH   ++H DIK ++ILL   GR  L D+G     C Q +  V K+ +  GT  + APE+     Y  + DIWSLGI++IEM++G+   Y  +  +    R+R            +S  LR  L   L  + + R+SA
Sbjct:    2 MNEEQIATVCLSVLKALSYLHTQGVIHRDIKSDSILLTSDGRIKLSDFG----FCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMVDGE-PPYFNEPPLQAMRRIRDNLPPRLKESHKVSSVLRAFLELMLVREPSQRASA 153

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Zebrafish
Match: pak6b (p21 protein (Cdc42/Rac)-activated kinase 6b [Source:ZFIN;Acc:ZDB-GENE-041014-121])

HSP 1 Score: 85.8853 bits (211), Expect = 1.668e-17
Identity = 54/157 (34.39%), Postives = 81/157 (51.59%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L  LH   ++H DIK ++ILL   GR  L D+G     C Q +  V K+ +  GT  + APE+     Y  + DIWSLGI+++EM++G+   Y  +  I+   RLR            IS  LR+ L   LT +   RSSA
Sbjct:  424 LTEEQIATVCESVLQALCYLHSQGVIHRDIKSDSILLTLDGRVKLSDFG----FCAQISKDVPKRRSLVGTPYWMAPEVVSKTPYGTEVDIWSLGIMVVEMVDGE-PPYFSETPISAMKRLRDEPPPTARNASKISPVLRDFLDSMLTREPQQRSSA 575

[Close]

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L  LH   ++H DIK ++ILL   GR  L D+G     C Q +  V K+ +  GT  + APE+     Y  + DIWSLGI+++EM++G+   Y  +  I+   RLR            IS  LR+ L   LT +   RSSA
Sbjct:  424 LTEEQIATVCESVLQALCYLHSQGVIHRDIKSDSILLTLDGRVKLSDFG----FCAQISKDVPKRRSLVGTPYWMAPEVVSKTPYGTEVDIWSLGIMVVEMVDGE-PPYFSETPISAMKRLRDEPPPTARNASKISPVLRDFLDSMLTREPQQRSSA 575

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Zebrafish
Match: pak6a (p21 protein (Cdc42/Rac)-activated kinase 6a [Source:ZFIN;Acc:ZDB-GENE-130410-1])

HSP 1 Score: 85.1149 bits (209), Expect = 2.860e-17
Identity = 50/157 (31.85%), Postives = 83/157 (52.87%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            +SE +I  +   +L+ L  LH   ++H DIK ++ILL   GR  L D+G     C Q +  + K+ +  GT  + APE+   + Y  + D+WSLGI+++EM++G+   +++      K RLR            +S  L++ L + LT D   R+SA
Sbjct:  510 LSEEQIATVCEAVLQALAYLHSQGVIHRDIKSDSILLSLDGRIKLSDFG----FCAQISKDIPKRKSLVGTPYWMAPEVISKSPYGTEVDVWSLGIMVVEMVDGEPPYFSETPVAAMK-RLRDELAPTVRNVHQVSPMLKDFLDRMLTRDPLERASA 661

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Zebrafish
Match: pak6a (p21 protein (Cdc42/Rac)-activated kinase 6a [Source:ZFIN;Acc:ZDB-GENE-130410-1])

HSP 1 Score: 85.1149 bits (209), Expect = 2.900e-17
Identity = 50/157 (31.85%), Postives = 83/157 (52.87%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            +SE +I  +   +L+ L  LH   ++H DIK ++ILL   GR  L D+G     C Q +  + K+ +  GT  + APE+   + Y  + D+WSLGI+++EM++G+   +++      K RLR            +S  L++ L + LT D   R+SA
Sbjct:  410 LSEEQIATVCEAVLQALAYLHSQGVIHRDIKSDSILLSLDGRIKLSDFG----FCAQISKDIPKRKSLVGTPYWMAPEVISKSPYGTEVDVWSLGIMVVEMVDGEPPYFSETPVAAMK-RLRDELAPTVRNVHQVSPMLKDFLDRMLTRDPLERASA 561

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Xenopus
Match: syt2 (synaptotagmin 2 [Source:Xenbase;Acc:XB-GENE-981211])

HSP 1 Score: 85.8853 bits (211), Expect = 2.679e-17
Identity = 54/157 (34.39%), Postives = 82/157 (52.23%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L+ LH   ++H DIK ++ILL   GR  L D+G     C Q +  V K+ +  GT  + APE+    SY  + DIWSLGI++IEM++G+   Y  D  +    RLR             S  LR+ L + L  D A R++A
Sbjct:  472 LNEEQIATVCKSVLQALEYLHSQGVIHRDIKSDSILLTLDGRIKLSDFG----FCAQISKDVPKRKSLVGTPYWMAPEVILRCSYGTEVDIWSLGIMVIEMVDGE-PPYFSDSPVQAMKRLRDSPPPKLKNSHKASPILRDFLERMLVRDAAERATA 623

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Xenopus
Match: syt2 (synaptotagmin 2 [Source:Xenbase;Acc:XB-GENE-981211])

HSP 1 Score: 85.8853 bits (211), Expect = 2.761e-17
Identity = 54/157 (34.39%), Postives = 82/157 (52.23%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L+ LH   ++H DIK ++ILL   GR  L D+G     C Q +  V K+ +  GT  + APE+    SY  + DIWSLGI++IEM++G+   Y  D  +    RLR             S  LR+ L + L  D A R++A
Sbjct:  490 LNEEQIATVCKSVLQALEYLHSQGVIHRDIKSDSILLTLDGRIKLSDFG----FCAQISKDVPKRKSLVGTPYWMAPEVILRCSYGTEVDIWSLGIMVIEMVDGE-PPYFSDSPVQAMKRLRDSPPPKLKNSHKASPILRDFLERMLVRDAAERATA 641

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Xenopus
Match: qsox2 (quiescin sulfhydryl oxidase 2 [Source:Xenbase;Acc:XB-GENE-986398])

HSP 1 Score: 84.3445 bits (207), Expect = 4.583e-17
Identity = 54/156 (34.62%), Postives = 79/156 (50.64%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            + E EI  +  E L+ L+ LH  +++H DIK +NILL   G   L D+G     C Q +   +K+    GT  + APE+    +Y  K D+WSLGI+ IEM+EG+   LN+      Y    N   + +     S   R+ L +CL  D+  R SA
Sbjct:  256 MQEGEIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGAVKLTDFG----FCAQISPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDVWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPQLQEPEKKSPAFRDFLSRCLEMDVEKRGSA 407

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Xenopus
Match: pou5f3.2 (POU class 5 homeobox 3, gene 2 [Source:Xenbase;Acc:XB-GENE-919841])

HSP 1 Score: 84.3445 bits (207), Expect = 7.029e-17
Identity = 51/156 (32.69%), Postives = 82/156 (52.56%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDK---------NINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +LK L VLH   ++H DIK ++ILL   GR  L D+G     C Q N  V ++ +  GT  + APE+     Y  + DIWSLGI++IEM++G+   + +           N+  K +  + +S  L+  L + L  D + R+SA
Sbjct:  404 MNEEQIATVCVSVLKALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFG----FCAQVNKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPKLKNAQKVSPLLKGFLDRLLVRDPSQRASA 555

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Xenopus
Match: qsox2 (quiescin sulfhydryl oxidase 2 [Source:Xenbase;Acc:XB-GENE-986398])

HSP 1 Score: 83.9593 bits (206), Expect = 7.272e-17
Identity = 54/156 (34.62%), Postives = 79/156 (50.64%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            + E EI  +  E L+ L+ LH  +++H DIK +NILL   G   L D+G     C Q +   +K+    GT  + APE+    +Y  K D+WSLGI+ IEM+EG+   LN+      Y    N   + +     S   R+ L +CL  D+  R SA
Sbjct:  295 MQEGEIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGAVKLTDFG----FCAQISPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDVWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPQLQEPEKKSPAFRDFLSRCLEMDVEKRGSA 446

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Mouse
Match: Pak1 (p21 (RAC1) activated kinase 1 [Source:MGI Symbol;Acc:MGI:1339975])

HSP 1 Score: 82.8037 bits (203), Expect = 1.453e-16
Identity = 55/157 (35.03%), Postives = 82/157 (52.23%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYG-CSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            + E +I  +  E L+ L+ LH  +++H DIK +NILL   G   L D+G C+++   Q+     K+    GT  + APE+    +Y  K DIWSLGI+ IEMIEG+   LN+      Y    N   + +    +S   R+ L +CL  D+  R SA
Sbjct:  360 MDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQS-----KRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSA 511

[Close]

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYG-CSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            + E +I  +  E L+ L+ LH  +++H DIK +NILL   G   L D+G C+++   Q+     K+    GT  + APE+    +Y  K DIWSLGI+ IEMIEG+   LN+      Y    N   + +    +S   R+ L +CL  D+  R SA
Sbjct:  360 MDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQS-----KRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSA 511

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Mouse
Match: Pak4 (p21 (RAC1) activated kinase 4 [Source:MGI Symbol;Acc:MGI:1917834])

HSP 1 Score: 82.8037 bits (203), Expect = 1.588e-16
Identity = 49/156 (31.41%), Postives = 82/156 (52.56%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDK-------NINFKFRLRFI---SENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L VLH   ++H DIK ++ILL   GR  L D+G     C Q +  V ++ +  GT  + APE+     Y  + DIWSLG+++IEM++G+   + +           N   RL+ +   S +L+  L + L  D A R++A
Sbjct:  414 MNEEQIAAVCLAVLQALAVLHAQGVIHRDIKSDSILLTHDGRVKLSDFG----FCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGVMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKASPSLKGFLDRLLVRDPAQRATA 565

[Close]

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDK-------NINFKFRLRFI---SENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L VLH   ++H DIK ++ILL   GR  L D+G     C Q +  V ++ +  GT  + APE+     Y  + DIWSLG+++IEM++G+   + +           N   RL+ +   S +L+  L + L  D A R++A
Sbjct:  414 MNEEQIAAVCLAVLQALAVLHAQGVIHRDIKSDSILLTHDGRVKLSDFG----FCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGVMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKASPSLKGFLDRLLVRDPAQRATA 565

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Mouse
Match: Pak6 (p21 (RAC1) activated kinase 6 [Source:MGI Symbol;Acc:MGI:2679420])

HSP 1 Score: 82.8037 bits (203), Expect = 1.813e-16
Identity = 51/157 (32.48%), Postives = 82/157 (52.23%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L  LH   ++H DIK ++ILL   GR  L D+G     C Q +  V K+ +  GT  + APE+   + Y+ + DIWSLGI++IEM++G+   Y  D  +    RLR            +S  LR+ L + L  +   R++A
Sbjct:  499 LNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFG----FCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGE-PPYFSDSPVQAMKRLRDSAPPKLKNSYKVSPVLRDFLDRMLVREPQERATA 650

[Close]

BLAST of Serine/threonine-protein kinase fused vs. UniProt/SwissProt
Match: sp|Q10056|SHK2_SCHPO (Serine/threonine-protein kinase shk2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=shk2 PE=3 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 6.124e-20
Identity = 59/157 (37.58%), Postives = 86/157 (54.78%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK-----------LNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAARSSA 614
            ++E +I ++  E  K ++ LH   I+H DIK +N+LLD SG   + D+G    + N+TN    K+    GT  + APE+ K N Y  K DIWSLGI+IIEMIE +           L   AK+     K +   +S+NL++ L  CLT D   R++A
Sbjct:  406 LTEAQIARICLETCKGIQHLHARNIIHRDIKSDNVLLDNSGNIKITDFGFCARLSNRTN----KRVTMVGTPYWMAPEVVKQNEYGTKVDIWSLGIMIIEMIENEPPYLREDPIRALYLIAKNGTPTLK-KPNLVSKNLKSFLNSCLTIDTIFRATA 557

[Close]

BLAST of Serine/threonine-protein kinase fused vs. UniProt/SwissProt
Match: sp|Q86IX1|DST1_DICDI (Serine/threonine-protein kinase dst1 OS=Dictyostelium discoideum OX=44689 GN=dst1 PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.632e-18
Identity = 61/162 (37.65%), Postives = 84/162 (51.85%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLRFI---------------SENLRNILMKCLTNDMAARSSA 614
            ISE+EI  +  E LK L  LH++K +H DIK  NILL+  G   L D+G S  + N        K N+F GT  + APE+ + N Y  KAD+WSLGI  IEM EG       + N++   R+ F+               S+  ++ + KCLT D A R +A
Sbjct:  121 ISEDEIALICREALKGLNYLHEFKKIHRDIKGGNILLNDRGEVKLADFGVSAQLFN-----TFSKRNTFVGTPYWMAPEVIQENKYDGKADVWSLGITAIEMAEG----LPPNSNVH-PMRVIFMIPREESPALTDKSIWSDKFQDFISKCLTKDPAERPTA 272

[Close]

BLAST of Serine/threonine-protein kinase fused vs. UniProt/SwissProt
Match: sp|O14427|CLA4_CANAX (Serine/threonine-protein kinase CLA4 OS=Candida albicans OX=5476 GN=CLA4 PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 4.748e-17
Identity = 59/157 (37.58%), Postives = 87/157 (55.41%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYG-CSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKF-RLRFISENLRNILMKCLTNDMAARSSA 614
            ++E +I  + +E LK L+ LHK  I+H DIK +N+LLD  G   + D+G C+KL  +Q N    K+    GT  + APE+ K   Y +K D+WSLGI+ IEMIEG+   LN+      Y    N   K  +   +S +++  L  CL  D+  R+S 
Sbjct:  775 LNEKQIATICFETLKGLQHLHKKHIIHRDIKSDNVLLDAYGNVKITDFGFCAKL-TDQRN----KRATMVGTPYWMAPEVVKQKEYDEKVDVWSLGIMTIEMIEGEPPYLNEEPLKALYLIATNGTPKLKKPELLSNSIKKFLSICLCVDVRYRAST 926

[Close]

BLAST of Serine/threonine-protein kinase fused vs. UniProt/SwissProt
Match: sp|Q5APR8|CLA4_CANAL (Serine/threonine-protein kinase CLA4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CLA4 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 4.845e-17
Identity = 59/157 (37.58%), Postives = 87/157 (55.41%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYG-CSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKF-RLRFISENLRNILMKCLTNDMAARSSA 614
            ++E +I  + +E LK L+ LHK  I+H DIK +N+LLD  G   + D+G C+KL  +Q N    K+    GT  + APE+ K   Y +K D+WSLGI+ IEMIEG+   LN+      Y    N   K  +   +S +++  L  CL  D+  R+S 
Sbjct:  780 LNEKQIATICFETLKGLQHLHKKHIIHRDIKSDNVLLDAYGNVKITDFGFCAKL-TDQRN----KRATMVGTPYWMAPEVVKQKEYDEKVDVWSLGIMTIEMIEGEPPYLNEEPLKALYLIATNGTPKLKKPELLSNSIKKFLSICLCVDVRYRAST 931

[Close]

BLAST of Serine/threonine-protein kinase fused vs. UniProt/SwissProt
Match: sp|Q9P7J8|GAD8_SCHPO (Serine/threonine-protein kinase gad8 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gad8 PE=1 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.988e-16
Identity = 53/136 (38.97%), Postives = 80/136 (58.82%), Query Frame = 3

Query:  210 EILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFR------LRF---ISENLRNILMKCLT 587
            E+L  L+ LH++ +++ D+KPENILLD +G   LCD+G  KL   +T+     + N+F GT +Y APE+   + Y+   D W+LG+++ EMI G    Y  D+NIN  +R      LRF   I E  +++L   LT
Sbjct:  336 ELLVALECLHEFNVIYRDLKPENILLDYTGHIALCDFGLCKLNMAKTD-----RTNTFCGTPEYLAPELLLGHGYTKVVDWWTLGVLLYEMITGLPPFY--DENINEMYRKILQDPLRFPDNIDEKAKDLLSGLLT 464

[Close]

BLAST of Serine/threonine-protein kinase fused vs. TrEMBL
Match: A0A2H1VRC7 (SFRICE_024974 OS=Spodoptera frugiperda OX=7108 GN=SFRICE_024974 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.905e-18
Identity = 52/153 (33.99%), Postives = 88/153 (57.52%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEG----------KLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAAR 605
            ++E+++ K+ ++++  L  LH  ++LH D+KP+N+LLD SGRA LCD+G ++++ N T+I+   K    GT  Y APE+ +   Y  +AD+WSLG ++ E+I G          +L +  + K + +     FIS + R+ L   L  D   R
Sbjct:   97 LNEDQVKKITWDLVSALYYLHSHRVLHRDLKPQNVLLDSSGRAKLCDFGLARIMTNATHILTSIK----GTPLYMAPELIEEKPYDHQADLWSLGCIVYELIAGQPPFCTMSIWQLVRMIRHKPVQWP---SFISSDARSFLQGLLHKDPMKR 242

[Close]

BLAST of Serine/threonine-protein kinase fused vs. TrEMBL
Match: A0A5J4WA07 (Putative NEK/NEK2 protein kinase OS=Streblomastix strix OX=222440 GN=EZS28_012766 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 4.953e-18
Identity = 52/144 (36.11%), Postives = 83/144 (57.64%), Query Frame = 3

Query:  207 YEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEG--------KLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAARSSA 614
            Y+IL  LK LH  +I+H D+KPENI LD+ G A   D+G + +I +++ +      N+ GTQ+Y+ PE    N  ++ +DIW+LG++++EMI G        +    A  KN  FK    ++ + L+ IL+  + +D A R SA
Sbjct:  122 YQILMVLKHLHSLRIVHRDLKPENIFLDQDGNAKTGDFGLALMIQSRSEV------NAAGTQNYQPPEAINFNQMTEASDIWALGVIVVEMITGVHPFQGRTQDETIANIKNGRFKALPDYVKDELKEILISMINSDPAKRPSA 259

[Close]

BLAST of Serine/threonine-protein kinase fused vs. TrEMBL
Match: A0A2W1BW45 (Protein kinase domain-containing protein OS=Helicoverpa armigera OX=29058 GN=HaOG202020 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 8.625e-18
Identity = 52/153 (33.99%), Postives = 86/153 (56.21%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEG----------KLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAAR 605
            ++E ++ K+ ++++  L  LH  ++LH D+KP+N+LLD SGRA LCD+G ++++ N T+I+   K    GT  Y APE+ +   Y  +AD+WSLG ++ E+I G          +L +  + K + +     FIS   R+ L   L  D   R
Sbjct:   97 LNEEQVKKITWDLVSALYYLHSHRVLHRDLKPQNVLLDSSGRAKLCDFGLARIMTNATHILTSIK----GTPLYMAPELIEEKPYDHQADLWSLGCIVYELIAGQPPFCTMSIWQLVRMIRHKPVQWP---SFISTEARSFLQGLLHKDPMKR 242

[Close]

BLAST of Serine/threonine-protein kinase fused vs. TrEMBL
Match: A0A437BWK5 (Protein kinase domain-containing protein OS=Chilo suppressalis OX=168631 GN=evm_000625 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.687e-17
Identity = 52/155 (33.55%), Postives = 89/155 (57.42%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEG----------KLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAARSS 611
            ++E+++ K+ ++++  L  LH  ++LH D+KP+N+LLD +GRA LCD+G ++++ N T+I+   K    GT  Y APE+ +   Y  +AD+WSLG ++ E++ G          +L +  K K + +     FIS + R+ L   L  D   R S
Sbjct:   97 LNEDQVKKITWDLVSALYYLHSHRVLHRDLKPQNVLLDSTGRAKLCDFGLARIMTNSTHILTSIK----GTPLYMAPELIEEKPYDHQADLWSLGCIVYELMAGQPPFCTMSIWQLVRMIKHKPVQWP---SFISIDARSFLQGLLHKDPMKRMS 244

[Close]

BLAST of Serine/threonine-protein kinase fused vs. TrEMBL
Match: A0A5J4VSA7 (Putative NEK/NEK2 protein kinase (Fragment) OS=Streblomastix strix OX=222440 GN=EZS28_019029 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.157e-17
Identity = 50/144 (34.72%), Postives = 83/144 (57.64%), Query Frame = 3

Query:  207 YEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEG--KLNQYAKD------KNINFKFRLRFISENLRNILMKCLTNDMAARSSA 614
            Y++L  LK LH  +I+H D+KPENI LD+ G A   D+G + +I +++ +      N+ GTQ+Y++PE    N  ++ +DIW+LG++++EMI G        +D      KN  FK    ++   L+ +L+  + +D A R SA
Sbjct:  122 YQVLMVLKHLHSLRIVHRDLKPENIFLDQDGNAKTGDFGLALMIQSRSEV------NAAGTQNYQSPEAINFNQMTEASDIWALGVIVVEMITGVHPFQGRTQDETVQNIKNGRFKALPDYVKGELKEMLISMINSDPAKRPSA 259

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Cavefish
Match: pak6b (serine/threonine-protein kinase PAK 6-like [Source:NCBI gene;Acc:103030407])

HSP 1 Score: 84.7297 bits (208), Expect = 3.093e-17
Identity = 52/157 (33.12%), Postives = 80/157 (50.96%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L  LH   ++H DIK ++ILL   GR  L D+G     C Q +  + K+ +  GT  + APE+     Y  + D+WSLGI+++EM++G+   Y  D  +    RLR            IS  L++ L   LT D   RSSA
Sbjct:  487 LTEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRIKLSDFG----FCAQISKDIPKRKSLVGTPYWMAPEVISKTPYGTEVDVWSLGIMVVEMVDGE-PPYFSDTPVAAMKRLRDEPAPSVRNSSKISPVLKDFLDSMLTRDPLQRSSA 638

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Cavefish
Match: pak1 (p21 protein (Cdc42/Rac)-activated kinase 1 [Source:ZFIN;Acc:ZDB-GENE-030826-29])

HSP 1 Score: 82.8037 bits (203), Expect = 9.446e-17
Identity = 55/157 (35.03%), Postives = 82/157 (52.23%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYG-CSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            + E +I  +  E L+ L+ LH  +++H DIK +NILL   G   L D+G C+++   Q+     K+    GT  + APE+    +Y  K DIWSLGI+ IEMIEG+   LN+      Y    N   + +    +S   R+ L +CL  D+  R SA
Sbjct:  313 MDEAQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQS-----KRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSSVFRDFLNRCLEMDVEKRGSA 464

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Cavefish
Match: pak1 (p21 protein (Cdc42/Rac)-activated kinase 1 [Source:ZFIN;Acc:ZDB-GENE-030826-29])

HSP 1 Score: 82.8037 bits (203), Expect = 1.022e-16
Identity = 55/157 (35.03%), Postives = 82/157 (52.23%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYG-CSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            + E +I  +  E L+ L+ LH  +++H DIK +NILL   G   L D+G C+++   Q+     K+    GT  + APE+    +Y  K DIWSLGI+ IEMIEG+   LN+      Y    N   + +    +S   R+ L +CL  D+  R SA
Sbjct:  339 MDEAQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQS-----KRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSSVFRDFLNRCLEMDVEKRGSA 490

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Cavefish
Match: pak1 (p21 protein (Cdc42/Rac)-activated kinase 1 [Source:ZFIN;Acc:ZDB-GENE-030826-29])

HSP 1 Score: 82.4185 bits (202), Expect = 1.407e-16
Identity = 55/157 (35.03%), Postives = 82/157 (52.23%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYG-CSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            + E +I  +  E L+ L+ LH  +++H DIK +NILL   G   L D+G C+++   Q+     K+    GT  + APE+    +Y  K DIWSLGI+ IEMIEG+   LN+      Y    N   + +    +S   R+ L +CL  D+  R SA
Sbjct:  320 MDEAQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQS-----KRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSSVFRDFLNRCLEMDVEKRGSA 471

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Cavefish
Match: pak6a (p21 (RAC1) activated kinase 6 [Source:NCBI gene;Acc:103035957])

HSP 1 Score: 82.8037 bits (203), Expect = 1.627e-16
Identity = 48/157 (30.57%), Postives = 83/157 (52.87%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            +SE +I  +   +L+ L  LH   ++H DIK ++ILL   GR  L D+G     C Q +  + K+ +  GT  + APE+   + Y  + D+WS+GI+++EM++G+   +++      K RLR            +S  L++ L + LT +   R+SA
Sbjct:  516 LSEEQIATVCEAVLQALAYLHSQGVIHRDIKSDSILLSLDGRIKLSDFG----FCAQISKDIPKRKSLVGTPYWMAPEVISKSPYGTEVDVWSMGIMVVEMVDGEPPYFSETPVAAMK-RLRDEPAPTIRNIQQVSPVLKDFLDRMLTREPMERASA 667

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004683.1 (pep scaffold:Pmarinus_7.0:GL488517:199:5283:1 gene:ENSPMAG00000004265.1 transcript:ENSPMAT00000004683.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 78.9518 bits (193), Expect = 1.186e-17
Identity = 44/115 (38.26%), Postives = 64/115 (55.65%), Query Frame = 3

Query:  174 LISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDK 515
            + +E ++     E+   L  LH   I++ D+KPENILLD+SG   L D+G SK      +I   KK  SF GT +Y APE+     ++  AD WS G+++ EM+ G L    KD+
Sbjct:   29 MFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDESGHIGLTDFGLSK-----ESIDHEKKAYSFCGTVEYMAPEVVNRKGHTHSADWWSFGVLMFEMLTGTLPFQGKDR 138

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Sea Lamprey
Match: pak4 (p21 protein (Cdc42/Rac)-activated kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040704-69])

HSP 1 Score: 80.1073 bits (196), Expect = 1.998e-16
Identity = 48/156 (30.77%), Postives = 81/156 (51.92%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDK---------NINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +L+ L  LH   ++H DIK ++ILL   GR  L D+G     C Q +  V ++ +  GT  + APE+     Y  + D+WSLGI++IEM++G+   + +           N+  K + ++  S  LR  L + L  D A R++A
Sbjct:  510 MNEEQIATVCVSVLRALAYLHGQGVIHRDIKSDSILLTADGRIKLSDFG----FCAQVSKEVTRRKSLVGTPYWMAPEVISRLPYGPEVDVWSLGIMVIEMVDGEPPYFNEPPLQAMRRIRDNVPPKMKNIQKASPLLRGFLDQMLVRDPALRATA 661

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007414.1 (pep scaffold:Pmarinus_7.0:GL476981:230882:244405:1 gene:ENSPMAG00000006689.1 transcript:ENSPMAT00000007414.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 74.7146 bits (182), Expect = 4.165e-15
Identity = 45/146 (30.82%), Postives = 75/146 (51.37%), Query Frame = 3

Query:  174 LISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAARSS 611
            L SE++I+    ++   LK +H  KILH DIK +NI L K+G   L D+G ++++ + T +         GT  Y +PEI +N  Y+ K+D+W+LG V+ EM                  R  F + N++N+++K +       S+
Sbjct:   99 LFSEDQILDWLVQLCLALKHVHDRKILHRDIKSQNIFLTKAGIVQLGDFGIARVLNSTTELAR----TCIGTPYYLSPEICENKPYNHKSDMWALGCVLYEMC---------------TLRHAFEAGNMKNLVLKIIRGSFPPVSA 225

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Sea Lamprey
Match: STK36 (serine/threonine kinase 36 [Source:HGNC Symbol;Acc:HGNC:17209])

HSP 1 Score: 76.2554 bits (186), Expect = 5.577e-15
Identity = 48/157 (30.57%), Postives = 77/157 (49.04%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRL------------RFISENLRNILMKCLTNDMAARSS 611
            + E ++ ++A +++  L  LH  +ILH D+KP+NILL K G   LCD+G ++ +   T ++   K    GT  Y +PE+ +   Y   AD+WS+G ++ E+  G    Y      N  F+L            + +S   RN L   LT +   R S
Sbjct:   97 LPEEQVREIACQLVSALYYLHSHRILHRDMKPQNILLGKGGVVKLCDFGFARAMSINTLVLTSIK----GTPLYMSPELVEEKPYDHTADLWSMGCILYELFVGTPPFYT-----NSIFQLVSLIIKDPVKWPKNMSSEFRNFLQGLLTKEPKLRLS 244

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Sea Lamprey
Match: PRKCD (protein kinase C delta [Source:HGNC Symbol;Acc:HGNC:9399])

HSP 1 Score: 74.7146 bits (182), Expect = 8.957e-15
Identity = 46/144 (31.94%), Postives = 71/144 (49.31%), Query Frame = 3

Query:  204 AYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFK-------FRLRFISENLRNILMKCLTNDMAARSSA 614
            A E++  L+ LH   I++ D+K +N+LLD+ G   + D+G  K      N+         GT DY APEI    +Y    D WS+G++I EM+ G+   Y  D++  F+          R++S++ RNIL      D   R  A
Sbjct:  359 AAEVICGLQFLHSHNIIYRDLKLDNVLLDREGHVKIADFGMCKEHVGAGNLAT----TFCGTPDYIAPEILLGRAYGTSVDWWSMGVLIYEMLIGQSPFYGSDEDELFEAIKGHTPHYPRWLSDSSRNILSCLFERDHTKRLGA 498

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Yeast
Match: TPK1 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication [Source:SGD;Acc:S000003700])

HSP 1 Score: 83.1889 bits (204), Expect = 6.007e-18
Identity = 50/149 (33.56%), Postives = 79/149 (53.02%), Query Frame = 3

Query:  186 NEIIKL-AYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEG--------KLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAAR 605
            N + K  A E+   L+ LH   I++ D+KPENILLDK+G   + D+G +K + + T  +        GT DY APE+     Y+   D WS GI+I EM+ G         +  Y K  N   +F   F +E+++++L + +T D++ R
Sbjct:  184 NPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILLDKNGHIKITDFGFAKYVPDVTYTLC-------GTPDYIAPEVVSTKPYNKSIDWWSFGILIYEMLAGYTPFYDSNTMKTYEKILNAELRFP-PFFNEDVKDLLSRLITRDLSQR 324

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Yeast
Match: MKK1 (MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication [Source:SGD;Acc:S000005757])

HSP 1 Score: 83.9593 bits (206), Expect = 6.955e-18
Identity = 43/106 (40.57%), Postives = 62/106 (58.49%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKL 494
            ISE  + K+A  +L+ L  LH+ K++H DIKP+NILL+++G+  LCD+G S    N             GT  Y APE  +   YS  +D+WSLG+ I+E+  GK 
Sbjct:  321 ISEKVLGKIAEAVLRGLSYLHEKKVIHRDIKPQNILLNENGQVKLCDFGVSGEAVNSLATTFT------GTSFYMAPERIQGQPYSVTSDVWSLGLTILEVANGKF 420

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Yeast
Match: SKM1 (Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication [Source:SGD;Acc:S000005473])

HSP 1 Score: 83.5741 bits (205), Expect = 1.073e-17
Identity = 58/166 (34.94%), Postives = 88/166 (53.01%), Query Frame = 3

Query:  147 RSNPNFRVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYG-CSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ------YAKDKNINFKFR-LRFISENLRNILMKCLTNDMAARSS 611
            RSN       ++EN++  +  E  + LK LH  KI+H DIK +NILL+  G   + D+G C +L   ++     K+    GT  + APEI     Y +K D+WSLGI++IEMIEG+   LN+      Y    N + K R    +S+  +  L  CL  ++ +R+S
Sbjct:  469 RSNTGEHSSPLNENQMAYIVKETCQGLKFLHNKKIIHRDIKSDNILLNSQGLVKITDFGFCVELTEKRS-----KRATMVGTPYWMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLNEDPLKALYLIANNGSPKLRHPESVSKQTKQFLDACLQVNVESRAS 629

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Yeast
Match: STE7 (Signal transducing MAP kinase kinase; involved in pheromone response where it phosphorylates Fus3p; involved in the pseudohyphal/invasive growth pathway where it phosphorylates of Kss1p; phosphorylated by Ste11p; degraded by ubiquitin pathway [Source:SGD;Acc:S000002318])

HSP 1 Score: 82.0333 bits (201), Expect = 2.863e-17
Identity = 43/103 (41.75%), Postives = 65/103 (63.11%), Query Frame = 3

Query:  192 IIKLAYEILKELKVLHK-WKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKL 494
            I K+AY +L  L  L++ +KI+H DIKP N+L++  G+  LCD+G SK + N          ++F GT  Y +PE  + N YS K D+WSLG++IIE++ G+ 
Sbjct:  307 ISKIAYGVLNGLDHLYRQYKIIHRDIKPSNVLINSKGQIKLCDFGVSKKLINSI-------ADTFVGTSTYMSPERIQGNVYSIKGDVWSLGLMIIELVTGEF 402

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Yeast
Match: MKK2 (MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication [Source:SGD;Acc:S000006061])

HSP 1 Score: 81.6481 bits (200), Expect = 3.410e-17
Identity = 43/110 (39.09%), Postives = 61/110 (55.45%), Query Frame = 3

Query:  165 RVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKL 494
            R   ISE  I K+A  +L+ L  LH+ K++H DIKP+NILL++ G   LCD+G S    N   +         GT  Y APE  +   YS   D+WSLG+ ++E+  G+ 
Sbjct:  310 RGGRISERVIGKIAESVLRGLSYLHERKVIHRDIKPQNILLNEKGEIKLCDFGVSGEAVNSLAMTFT------GTSFYMAPERIQGQPYSVTCDVWSLGLTLLEVAGGRF 413

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Nematostella
Match: EDO45846 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRL1])

HSP 1 Score: 77.0258 bits (188), Expect = 9.381e-16
Identity = 51/172 (29.65%), Postives = 86/172 (50.00%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSAXXXXXXXVFKDIQNT 659
            +SE ++  +   +LK L  LH   ++H DIK ++ILL  +G   L D+G     C Q    + ++ +  GT  + APE+     Y  + DIWSLGI+++EM++G+   Y  +  +    +LR            IS  L++ L   L  D   R++A ELL     +++ N+
Sbjct:  120 LSEEQVACVCRAVLKALTFLHSQGVIHRDIKSDSILLTSNGTVKLSDFG----FCAQVTEDMPRRKSLVGTPYWMAPEVISRKPYGTEVDIWSLGIMVLEMVDGE-PPYFNEPPLQAMRKLRDLEPPTSRNPIQISPRLQSFLDNALIRDPLYRATAFELLNHPFIRNVGNS 286

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Nematostella
Match: EDO31934 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWI4])

HSP 1 Score: 77.7962 bits (190), Expect = 1.054e-15
Identity = 44/106 (41.51%), Postives = 62/106 (58.49%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK 491
            I E  I  +  E+LK L  LH  K LH DIK  N+L+ ++G   L D+G +  + +  N     K N+F GT  + APE+ K ++Y  KADIWSLGI  IE+ +G+
Sbjct:  103 IEEFYIATILREVLKGLDYLHTEKKLHRDIKAANVLMSETGDVKLADFGVAGQLTDTLN-----KRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELAKGE 203

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Nematostella
Match: EDO33667 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRJ0])

HSP 1 Score: 77.0258 bits (188), Expect = 3.093e-15
Identity = 39/105 (37.14%), Postives = 58/105 (55.24%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK 491
            + E +I  +  E L+ L+ LH   ++H DIK +NILL   G+  L D+G    I  + N    K+    GT  + APE+     Y  + D+WSLGI+ IEM+EG+
Sbjct:  317 MDEGQIAAVCRECLQALEFLHSNGVIHRDIKSDNILLGMDGQVKLTDFGFCATITPEQN----KRSTMVGTPYWMAPEVVTRKQYGPRVDVWSLGIMAIEMVEGE 417

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Nematostella
Match: EDO37800 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEW2])

HSP 1 Score: 75.8702 bits (185), Expect = 5.674e-15
Identity = 52/142 (36.62%), Postives = 73/142 (51.41%), Query Frame = 3

Query:  204 AYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDK-----NINFK-FRLRF-ISENLRNILMKCLTNDMAAR 605
            A EI   +  LH  +I++ D+KPENILLD  G   L D+G  K   +++        ++F GT +Y APE+ +   Y    D W LG V+ EM+ G    Y++D      NI  K  RLR  IS++ R+IL   L  D   R
Sbjct:  179 AAEIASAIGYLHGLQIIYRDLKPENILLDSKGHIVLTDFGLCKEGIDRSGTT-----STFCGTPEYLAPEVLRKQDYDKSVDWWCLGAVLYEMMYGLPPFYSRDTAEMYDNILHKPLRLRTNISQSARHILEGLLQKDKTKR 315

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Nematostella
Match: EDO31895 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWM6])

HSP 1 Score: 75.485 bits (184), Expect = 1.043e-14
Identity = 43/115 (37.39%), Postives = 62/115 (53.91%), Query Frame = 3

Query:  174 LISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF-GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDK 515
            + +E ++     E+   L  LH   I++ D+KPENILLD SG   L D+G SK   +       +K  SF GT +Y APE+     +S  AD WS G+++ EM+ G L    KD+
Sbjct:  105 MFTEEDVKFYLAELALALDHLHGLGIIYRDLKPENILLDASGHVALTDFGLSKESEHD------QKTYSFCGTVEYMAPEVVNRRGHSQAADWWSYGVLMFEMLTGSLPFQGKDR 213

HSP 2 Score: 58.151 bits (139), Expect = 3.646e-9
Identity = 32/108 (29.63%), Postives = 55/108 (50.93%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILL-DKSGRAT---LCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEG 488
            +SE E   + Y +   +  LH+  ++H D+KP NI+  D SG      + D+G +K +     +++        T ++ APE+ K   Y    DIWSLG+++  M+ G
Sbjct:  445 LSEREAGNIMYTLTSTIAFLHEEGVVHRDLKPSNIMYADDSGTPESLRIVDFGFAKQMRATNGLLM----TPCYTANFVAPEVLKKQGYDAACDIWSLGVLLYTMLSG 548

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Medaka
Match: pak6a (p21 (RAC1) activated kinase 6 [Source:NCBI gene;Acc:101175447])

HSP 1 Score: 84.7297 bits (208), Expect = 3.951e-17
Identity = 49/157 (31.21%), Postives = 83/157 (52.87%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLR-----------FISENLRNILMKCLTNDMAARSSA 614
            +SE +I  +   +L+ L  LH   ++H DIK ++ILL   GR  L D+G     C Q +  + K+ +  GT  + APE+   + Y  + D+WS+GI+++EM++G+   +++      K RLR            +S  L++ L + LT D   R+SA
Sbjct:  512 LSEEQIATVCEAVLQALAYLHSQGVIHRDIKSDSILLTLDGRVKLSDFG----FCAQISKDIPKRKSLVGTPYWMAPEVISKSPYGTEVDVWSMGIMVVEMVDGEPPYFSETPVAAMK-RLRDEQAPTVRNISQVSPVLKHFLDRMLTRDPLERASA 663

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Medaka
Match: ENSORLT00000044704.1 (serine/threonine-protein kinase 4 [Source:NCBI gene;Acc:101175466])

HSP 1 Score: 83.9593 bits (206), Expect = 4.538e-17
Identity = 57/157 (36.31%), Postives = 79/157 (50.32%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAK----------DKNINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            +SE EI  + +  LK L+ LH  + +H DIK  NILL+  G+A L D+G    +  Q    + K+    GT  + APE+ +   Y+  ADIWSLGI  IEM EGK   YA             N    FR     SEN R+ + +CL  +   R++A
Sbjct:  124 LSEEEIATVVHSTLKGLEYLHFMRKIHRDIKAGNILLNAEGQAKLADFG----VAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGK-PPYADIHPMRAIFMIPTNPPPTFRNPDLWSENFRDFVSQCLVKNPENRATA 275

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Medaka
Match: ENSORLT00000024938.2 (serine/threonine-protein kinase 4 [Source:NCBI gene;Acc:101175466])

HSP 1 Score: 83.5741 bits (205), Expect = 5.610e-17
Identity = 57/157 (36.31%), Postives = 79/157 (50.32%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAK----------DKNINFKFR-LRFISENLRNILMKCLTNDMAARSSA 614
            +SE EI  + +  LK L+ LH  + +H DIK  NILL+  G+A L D+G    +  Q    + K+    GT  + APE+ +   Y+  ADIWSLGI  IEM EGK   YA             N    FR     SEN R+ + +CL  +   R++A
Sbjct:  124 LSEEEIATVVHSTLKGLEYLHFMRKIHRDIKAGNILLNAEGQAKLADFG----VAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGK-PPYADIHPMRAIFMIPTNPPPTFRNPDLWSENFRDFVSQCLVKNPENRATA 275

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Medaka
Match: pak4 (p21 (RAC1) activated kinase 4 [Source:NCBI gene;Acc:101162543])

HSP 1 Score: 82.8037 bits (203), Expect = 1.136e-16
Identity = 50/156 (32.05%), Postives = 80/156 (51.28%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEG----------KLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAARSSA 614
            ++E +I  +   +LK L VLH   ++H DIK ++ILL   GR  L D+G     C Q +  V ++ +  GT  + APE+     Y  + DIWSLGI++IEM++G          K  +  +D +      L  +S  L+  L + L  + A R++A
Sbjct:  389 MNEEQIATVCVSVLKALSVLHSQGVIHRDIKSDSILLTHDGRVKLSDFG----FCAQVSKEVQRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNSPPKLKNLHKVSPLLKGFLDRMLVREPAQRATA 540

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Medaka
Match: PAK3 (serine/threonine-protein kinase PAK 3 [Source:NCBI gene;Acc:101162700])

HSP 1 Score: 82.8037 bits (203), Expect = 1.302e-16
Identity = 54/157 (34.39%), Postives = 78/157 (49.68%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGK---LNQ--------YAKDKNINFKFRLRFISENLRNILMKCLTNDMAARSSA 614
            + E +I  +  E L+ L  LH  +++H DIK +NILL   G   L D+G     C Q     +K+    GT  + APE+    +Y  K DIWSLGI+ IEM+EG+   LN+         A +     +   R +S   R+ L +CL  D+  R SA
Sbjct:  370 MDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFG----FCAQITPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPER-LSSVFRDFLNRCLEMDVDRRGSA 521

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Planmine SMEST
Match: SMESG000078507.1 (SMESG000078507.1)

HSP 1 Score: 419.083 bits (1076), Expect = 4.483e-141
Identity = 217/219 (99.09%), Postives = 218/219 (99.54%), Query Frame = 3

Query:    3 VRDININEEIKYLELFLKYRGLMSYNKKICAAFSLFEKSLDNKIKITIRSNPNFRVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAARSSAXXXXXXXVFKDIQNT 659
            VR+ININEEIKYLELF KYRGLMSYNKKICAAFSLFEKSLDNKIKITIRSNPNFRVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAARSSAEELLELEVFKDIQNT
Sbjct:  458 VREININEEIKYLELFPKYRGLMSYNKKICAAFSLFEKSLDNKIKITIRSNPNFRVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAARSSAEELLELEVFKDIQNT 676

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Planmine SMEST
Match: SMESG000078509.1 (SMESG000078509.1)

HSP 1 Score: 369.777 bits (948), Expect = 1.415e-128
Identity = 191/197 (96.95%), Postives = 192/197 (97.46%), Query Frame = 3

Query:   69 MSYNKKICAAFSLFEKSLDNKIKITIRSNPNFRVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAARSSAXXXXXXXVFKDIQNT 659
            MSYNKKICAAF LFEKSLDNKIKITIRSNPNFRVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEIS+NNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINF FRLRFISENLRNILMKCL  D AARSSAEELLELEVFKDIQNT
Sbjct:    1 MSYNKKICAAFLLFEKSLDNKIKITIRSNPNFRVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISENNSYSDKADIWSLGIVIIEMIEGKLNQYAKDKNINFAFRLRFISENLRNILMKCLITDPAARSSAEELLELEVFKDIQNT 197

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Planmine SMEST
Match: SMESG000078505.1 (SMESG000078505.1)

HSP 1 Score: 202.986 bits (515), Expect = 1.189e-58
Identity = 128/222 (57.66%), Postives = 162/222 (72.97%), Query Frame = 3

Query:    3 VRDININEEIKYLELFLKYRGLMSYNKKICAAFSLFEKSLDNKIKITIRSNPNFRVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSD--KADIWSLGIVIIEMIEGKLNQYAK-DKNINFKFRLRFISENLRNILMKCLTNDMAARSSAXXXXXXXVFKDIQNT 659
            VR+ININEEIKY +L   YRGLM Y+++IC AFSLFE+SLD KIK   + +        +E +I+++AYEILKELK LHKW+I+H DIKPENILLDK+GRATLCDYGCSK+IC+QT++ ++ K +  GT  Y APE+ K    SD  K+DIWSLGIVIIEM+EGKL+   K    IN +  L+F S  L+ IL KCL  D   R SA+ELL+L+ FKDIQ+ 
Sbjct:  399 VREININEEIKYFKLLSNYRGLMRYDREICVAFSLFEESLDKKIKSYCQKSEE------NEKKILRVAYEILKELKELHKWEIIHRDIKPENILLDKNGRATLCDYGCSKVICSQTSLQIN-KSDLAGTLGYIAPELIKGKPQSDKTKSDIWSLGIVIIEMMEGKLSSDIKFSHKINLE-ALQFKSVKLKKILKKCLKTDPDKRLSAKELLQLDAFKDIQSA 612

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Planmine SMEST
Match: SMESG000078505.1 (SMESG000078505.1)

HSP 1 Score: 202.216 bits (513), Expect = 4.275e-58
Identity = 128/222 (57.66%), Postives = 162/222 (72.97%), Query Frame = 3

Query:    3 VRDININEEIKYLELFLKYRGLMSYNKKICAAFSLFEKSLDNKIKITIRSNPNFRVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSD--KADIWSLGIVIIEMIEGKLNQYAK-DKNINFKFRLRFISENLRNILMKCLTNDMAARSSAXXXXXXXVFKDIQNT 659
            VR+ININEEIKY +L   YRGLM Y+++IC AFSLFE+SLD KIK   + +        +E +I+++AYEILKELK LHKW+I+H DIKPENILLDK+GRATLCDYGCSK+IC+QT++ ++ K +  GT  Y APE+ K    SD  K+DIWSLGIVIIEM+EGKL+   K    IN +  L+F S  L+ IL KCL  D   R SA+ELL+L+ FKDIQ+ 
Sbjct:  448 VREININEEIKYFKLLSNYRGLMRYDREICVAFSLFEESLDKKIKSYCQKSEE------NEKKILRVAYEILKELKELHKWEIIHRDIKPENILLDKNGRATLCDYGCSKVICSQTSLQIN-KSDLAGTLGYIAPELIKGKPQSDKTKSDIWSLGIVIIEMMEGKLSSDIKFSHKINLE-ALQFKSVKLKKILKKCLKTDPDKRLSAKELLQLDAFKDIQSA 661

[Close]

BLAST of Serine/threonine-protein kinase fused vs. Planmine SMEST
Match: SMESG000024565.1 (SMESG000024565.1)

HSP 1 Score: 103.219 bits (256), Expect = 9.877e-25
Identity = 63/158 (39.87%), Postives = 92/158 (58.23%), Query Frame = 3

Query:  177 ISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGRATLCDYGCSKLICNQTNIIVHKKPNSF--GTQDYEAPEISKNNSYSDKADIWSLGIVIIEMIEGKLNQYAK---------DKNINFKFRLR-FISENLRNILMKCLTNDMAARSSA 614
            I ENE IK++Y+IL ++  LH+ +I+H DIKP+NI+LD +G A L D+G SK + N T  I+ +K  ++  GT +Y APEI +   YS K DIWS G+V++EM+ G    + K          K + ++F+    IS      +  CLT D   R SA
Sbjct:   93 IHENEKIKISYQILTQIHELHRLQIVHRDIKPDNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPPRVVIELKKMVEYEFKPNLLISIEFNQFIKWCLTVDPNERPSA 250

The following BLAST results are available for this feature:

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Human
BLAST of Serine/threonine-protein kinase fused vs. Ensembl Celegans
BLAST of Serine/threonine-protein kinase fused vs. Ensembl Fly
BLAST of Serine/threonine-protein kinase fused vs. Ensembl Zebrafish
BLAST of Serine/threonine-protein kinase fused vs. Ensembl Xenopus
BLAST of Serine/threonine-protein kinase fused vs. Ensembl Mouse
BLAST of Serine/threonine-protein kinase fused vs. UniProt/SwissProt
BLAST of Serine/threonine-protein kinase fused vs. TrEMBL
BLAST of Serine/threonine-protein kinase fused vs. Ensembl Cavefish
BLAST of Serine/threonine-protein kinase fused vs. Ensembl Sea Lamprey
BLAST of Serine/threonine-protein kinase fused vs. Ensembl Yeast
BLAST of Serine/threonine-protein kinase fused vs. Ensembl Nematostella
BLAST of Serine/threonine-protein kinase fused vs. Ensembl Medaka
BLAST of Serine/threonine-protein kinase fused vs. Planmine SMEST

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
PAK1	1.034e-17	35.03	p21 (RAC1) activated kinase 1 [Source:HGNC Symbol;...	[more]
STK36	1.890e-17	32.24	serine/threonine kinase 36 [Source:HGNC Symbol;Acc...	[more]
PAK4	2.281e-17	31.41	p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;...	[more]
PAK4	2.281e-17	31.41	p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;...	[more]
PAK4	3.614e-17	31.41	p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;...	[more]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pak-2	3.202e-17	30.56	Serine/threonine-protein kinase pak-2 [Source:Uni...	[more]
pak-2	3.486e-17	30.56	Serine/threonine-protein kinase pak-2 [Source:Uni...	[more]
cst-1	4.565e-16	44.04	Serine/threonine-protein kinase cst-1 Serine/threo...	[more]
cst-1	4.716e-16	44.04	Serine/threonine-protein kinase cst-1 Serine/threo...	[more]
cst-2	5.491e-16	44.04	Caenorhabditis STE20-like kinase [Source:UniProtK...	[more]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Akt1	8.876e-16	32.24	gene:FBgn0010379 transcript:FBtr0083226	[more]
Akt1	8.876e-16	32.24	gene:FBgn0010379 transcript:FBtr0344470	[more]
Akt1	8.876e-16	32.24	gene:FBgn0010379 transcript:FBtr0344717	[more]
Akt1	8.876e-16	32.24	gene:FBgn0010379 transcript:FBtr0083227	[more]
Akt1	1.252e-15	32.24	gene:FBgn0010379 transcript:FBtr0083228	[more]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pak7	7.189e-18	32.48	p21 protein (Cdc42/Rac)-activated kinase 7 [Source...	[more]
pak6b	1.668e-17	34.39	p21 protein (Cdc42/Rac)-activated kinase 6b [Sourc...	[more]
pak6b	1.668e-17	34.39	p21 protein (Cdc42/Rac)-activated kinase 6b [Sourc...	[more]
pak6a	2.860e-17	31.85	p21 protein (Cdc42/Rac)-activated kinase 6a [Sourc...	[more]
pak6a	2.900e-17	31.85	p21 protein (Cdc42/Rac)-activated kinase 6a [Sourc...	[more]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
syt2	2.679e-17	34.39	synaptotagmin 2 [Source:Xenbase;Acc:XB-GENE-981211...	[more]
syt2	2.761e-17	34.39	synaptotagmin 2 [Source:Xenbase;Acc:XB-GENE-981211...	[more]
qsox2	4.583e-17	34.62	quiescin sulfhydryl oxidase 2 [Source:Xenbase;Acc:...	[more]
pou5f3.2	7.029e-17	32.69	POU class 5 homeobox 3, gene 2 [Source:Xenbase;Acc...	[more]
qsox2	7.272e-17	34.62	quiescin sulfhydryl oxidase 2 [Source:Xenbase;Acc:...	[more]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Pak1	1.453e-16	35.03	p21 (RAC1) activated kinase 1 [Source:MGI Symbol;A...	[more]
Pak1	1.453e-16	35.03	p21 (RAC1) activated kinase 1 [Source:MGI Symbol;A...	[more]
Pak4	1.588e-16	31.41	p21 (RAC1) activated kinase 4 [Source:MGI Symbol;A...	[more]
Pak4	1.588e-16	31.41	p21 (RAC1) activated kinase 4 [Source:MGI Symbol;A...	[more]
Pak6	1.813e-16	32.48	p21 (RAC1) activated kinase 6 [Source:MGI Symbol;A...	[more]

BLAST of Serine/threonine-protein kinase fused vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q10056\|SHK2_SCHPO	6.124e-20	37.58	Serine/threonine-protein kinase shk2 OS=Schizosacc...	[more]
sp\|Q86IX1\|DST1_DICDI	1.632e-18	37.65	Serine/threonine-protein kinase dst1 OS=Dictyostel...	[more]
sp\|O14427\|CLA4_CANAX	4.748e-17	37.58	Serine/threonine-protein kinase CLA4 OS=Candida al...	[more]
sp\|Q5APR8\|CLA4_CANAL	4.845e-17	37.58	Serine/threonine-protein kinase CLA4 OS=Candida al...	[more]
sp\|Q9P7J8\|GAD8_SCHPO	1.988e-16	38.97	Serine/threonine-protein kinase gad8 OS=Schizosacc...	[more]

BLAST of Serine/threonine-protein kinase fused vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A2H1VRC7	2.905e-18	33.99	SFRICE_024974 OS=Spodoptera frugiperda OX=7108 GN=...	[more]
A0A5J4WA07	4.953e-18	36.11	Putative NEK/NEK2 protein kinase OS=Streblomastix ...	[more]
A0A2W1BW45	8.625e-18	33.99	Protein kinase domain-containing protein OS=Helico...	[more]
A0A437BWK5	1.687e-17	33.55	Protein kinase domain-containing protein OS=Chilo ...	[more]
A0A5J4VSA7	2.157e-17	34.72	Putative NEK/NEK2 protein kinase (Fragment) OS=Str...	[more]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pak6b	3.093e-17	33.12	serine/threonine-protein kinase PAK 6-like [Source...	[more]
pak1	9.446e-17	35.03	p21 protein (Cdc42/Rac)-activated kinase 1 [Source...	[more]
pak1	1.022e-16	35.03	p21 protein (Cdc42/Rac)-activated kinase 1 [Source...	[more]
pak1	1.407e-16	35.03	p21 protein (Cdc42/Rac)-activated kinase 1 [Source...	[more]
pak6a	1.627e-16	30.57	p21 (RAC1) activated kinase 6 [Source:NCBI gene;Ac...	[more]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSPMAT00000004683.1	1.186e-17	38.26	pep scaffold:Pmarinus_7.0:GL488517:199:5283:1 gene...	[more]
pak4	1.998e-16	30.77	p21 protein (Cdc42/Rac)-activated kinase 4 [Source...	[more]
ENSPMAT00000007414.1	4.165e-15	30.82	pep scaffold:Pmarinus_7.0:GL476981:230882:244405:1...	[more]
STK36	5.577e-15	30.57	serine/threonine kinase 36 [Source:HGNC Symbol;Acc...	[more]
PRKCD	8.957e-15	31.94	protein kinase C delta [Source:HGNC Symbol;Acc:HGN...	[more]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
TPK1	6.007e-18	33.56	cAMP-dependent protein kinase catalytic subunit; p...	[more]
MKK1	6.955e-18	40.57	MAPKK involved in the protein kinase C signaling p...	[more]
SKM1	1.073e-17	34.94	Member of the PAK family of serine/threonine prote...	[more]
STE7	2.863e-17	41.75	Signal transducing MAP kinase kinase; involved in ...	[more]
MKK2	3.410e-17	39.09	MAPKK involved in the protein kinase C signaling p...	[more]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO45846	9.381e-16	29.65	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO31934	1.054e-15	41.51	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO33667	3.093e-15	37.14	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO37800	5.674e-15	36.62	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO31895	1.043e-14	37.39	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Serine/threonine-protein kinase fused vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pak6a	3.951e-17	31.21	p21 (RAC1) activated kinase 6 [Source:NCBI gene;Ac...	[more]
ENSORLT00000044704.1	4.538e-17	36.31	serine/threonine-protein kinase 4 [Source:NCBI gen...	[more]
ENSORLT00000024938.2	5.610e-17	36.31	serine/threonine-protein kinase 4 [Source:NCBI gen...	[more]
pak4	1.136e-16	32.05	p21 (RAC1) activated kinase 4 [Source:NCBI gene;Ac...	[more]
PAK3	1.302e-16	34.39	serine/threonine-protein kinase PAK 3 [Source:NCBI...	[more]

BLAST of Serine/threonine-protein kinase fused vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000078507.1	4.483e-141	99.09	SMESG000078507.1	[more]
SMESG000078509.1	1.415e-128	96.95	SMESG000078509.1	[more]
SMESG000078505.1	1.189e-58	57.66	SMESG000078505.1	[more]
SMESG000078505.1	4.275e-58	57.66	SMESG000078505.1	[more]
SMESG000024565.1	9.877e-25	39.87	SMESG000024565.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30035808 ID=SMED30035808|Name=Serine/threonine-protein kinase fused|organism=Schmidtea mediterranea sexual|type=transcript|length=1308bp
GCGTCCGCGATATTAATATAAATGAAGAAATAAAATATTTGGAATTGTTT
CTAAAGTATCGCGGGTTGATGAGCTATAACAAAAAGATCTGTGCCGCATT
CTCCTTATTTGAAAAATCGTTGGACAACAAAATAAAAATAACTATTAGAA
GTAATCCTAATTTTCGAGTTCCTCTAATTAGCGAAAATGAGATTATTAAG
TTGGCATACGAAATCCTCAAAGAATTGAAAGTGCTCCACAAATGGAAAAT
TCTTCATCTCGATATAAAACCCGAAAATATTCTGTTGGATAAAAGTGGAA
GGGCTACCCTATGTGATTATGGCTGCTCAAAACTGATTTGTAATCAAACA
AATATAATAGTACATAAGAAACCAAATTCCTTTGGAACTCAGGACTACGA
GGCTCCGGAAATATCAAAAAATAATTCTTATTCTGATAAAGCCGACATTT
GGTCGCTTGGCATTGTAATAATTGAAATGATAGAAGGAAAATTAAATCAA
TACGCAAAGGATAAAAATATCAATTTTAAATTTCGTTTGCGATTTATTTC
GGAAAATTTAAGAAATATTTTAATGAAATGCCTAACTAATGATATGGCTG
CAAGATCATCTGCAGAAGAACTTCTAGAACTGGAAGTTTTCAAGGATATT
CAGAACACATAATTAATTAAATTAATAATTTGACAGCATTCATTAAAAAT
AATTCGAATGAAATGAAAATTTTATGTTATAAAAATTATTTATTATTATT
ACTTTTGTTATGATTGTTATAAGAACATTTTTTGATTTTCAACTCTGATT
TCTTTTAATTTATAAAAAACATTGTATTTTCTAAAACATTTGGCTTTGAA
ACNGNTTTNAATATTTTCAACTCTGTTGTTGTTGTCGTCAGTGTTTTTAA
AGCACGGTATGTAACGAGAATGCAGTTCTGAAGGAAAGAAAGAGATGCAG
AGAGAGCAAAGGCTGAGAGGAACTGATGTATTTCTATTTTTTTTAAAGAA
AATGTTTTTAATTGTTTCTGTTTTGTAAATGTGAGGCTTTGAAAAGTGTG
AAACTCCAAGGATCCGAAAACGTTGGACATCGCTATGGAACAAAACAAAT
GTATATTTTGCTAAGTGAAAAACACTATCTTACAGCTGAAAGAATTTTTG
TTTTCCTAGGTTTAGTTTTACGTGGGATTTTCTTTTTCCTCCTGTATAAT
ATGTAAATATCGCGAGCTGAACCTGGCTGCCATGCGCAGCCTGGCTCCGA
GGAGCAGCTCCAGGTCCTGGACTTGGTGCCGCAGTGTGAGCAATACTCTC
CAGCAGAA

protein sequence of SMED30035808-orf-1

>SMED30035808-orf-1 ID=SMED30035808-orf-1|Name=SMED30035808-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=220bp
VRDININEEIKYLELFLKYRGLMSYNKKICAAFSLFEKSLDNKIKITIRS
NPNFRVPLISENEIIKLAYEILKELKVLHKWKILHLDIKPENILLDKSGR
ATLCDYGCSKLICNQTNIIVHKKPNSFGTQDYEAPEISKNNSYSDKADIW
SLGIVIIEMIEGKLNQYAKDKNINFKFRLRFISENLRNILMKCLTNDMAA
RSSAEELLELEVFKDIQNT*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037	heme binding
GO:0004672	protein kinase activity
GO:0005524	ATP binding
GO:0000166	nucleotide binding
GO:0004674	protein serine/threonine kinase activity
GO:0016301	kinase activity
GO:0016740	transferase activity

Vocabulary: **biological process**
Term	Definition
GO:0055114	oxidation-reduction process
GO:0006468	protein phosphorylation
GO:0016310	phosphorylation

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000070	intestinal phagocyte
PLANA:0000421	tail

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR008271	Ser/Thr_kinase_AS
IPR011009	Kinase-like_dom_sf

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 1..213 e-value: 7.9E-12 score: 35.2
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 2..212 e-value: 5.0E-34 score: 117.9
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..213 score: 28.994
None	No IPR available	GENE3D	G3DSA:1.10.510.10		coord: 55..219 e-value: 6.7E-40 score: 138.5
None	No IPR available	PIRSF	PIRSF000654	ILK	coord: 55..218 e-value: 3.0E-20 score: 69.9
None	No IPR available	PANTHER	PTHR44305	FAMILY NOT NAMED	coord: 26..209
None	No IPR available	PANTHER	PTHR44305:SF12	TYROSINE-PROTEIN KINASE C03B1.5-RELATED	coord: 26..209
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 83..95
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	SSF56112	Protein kinase-like (PK-like)	coord: 2..217