Threonine--tRNA ligase, cytoplasmic
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035804 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Human
Match: TARS1 (threonyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:11572]) HSP 1 Score: 504.212 bits (1297), Expect = 3.760e-170 Identity = 245/504 (48.61%), Postives = 335/504 (66.47%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K D K IKV +G + K TPY I + L+++ +I+KVN + WDL++PL D +E L FE++ A+ +W S+A ++ +E ++ + GF D L+ +SS F S+E + FER+++ E +F ++ K ++ E++++ ++YRC LID+C P VR+TG++K LKI ++ S + +LQRIYG+SFP+ ++L W QEEA R+HR IG +QEL+FFH SPGS F LP+G +I N LI+ IR +YRKRGF EV TP IF + LW TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE EI+ LDF++ VY + F F L+LSTRP+ FLGDIE+W+ AE+QL+ SL +FG+ W LNSGDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 75 KAEKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLEALCKKIIKEKQAFERLEVKKETLLAMFKYNKFKCRILNEKVNTPT--TTVYRCGPLIDLCRGPHVRHTGKIKALKIHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVE-KELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 575 HSP 2 Score: 115.931 bits (289), Expect = 3.760e-170 Identity = 60/126 (47.62%), Postives = 82/126 (65.08%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVI+HRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V EYA +V QQ DA F+ D DL + +KI N + +YN IL++G+KE S V++R + H + E I++ +
Sbjct: 584 KRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQ 709
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Human
Match: TARS1 (threonyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:11572]) HSP 1 Score: 504.212 bits (1297), Expect = 3.760e-170 Identity = 245/504 (48.61%), Postives = 335/504 (66.47%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K D K IKV +G + K TPY I + L+++ +I+KVN + WDL++PL D +E L FE++ A+ +W S+A ++ +E ++ + GF D L+ +SS F S+E + FER+++ E +F ++ K ++ E++++ ++YRC LID+C P VR+TG++K LKI ++ S + +LQRIYG+SFP+ ++L W QEEA R+HR IG +QEL+FFH SPGS F LP+G +I N LI+ IR +YRKRGF EV TP IF + LW TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE EI+ LDF++ VY + F F L+LSTRP+ FLGDIE+W+ AE+QL+ SL +FG+ W LNSGDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 75 KAEKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLEALCKKIIKEKQAFERLEVKKETLLAMFKYNKFKCRILNEKVNTPT--TTVYRCGPLIDLCRGPHVRHTGKIKALKIHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVE-KELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 575 HSP 2 Score: 115.931 bits (289), Expect = 3.760e-170 Identity = 60/126 (47.62%), Postives = 82/126 (65.08%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVI+HRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V EYA +V QQ DA F+ D DL + +KI N + +YN IL++G+KE S V++R + H + E I++ +
Sbjct: 584 KRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQ 709
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Human
Match: TARS3 (threonyl-tRNA synthetase 3 [Source:HGNC Symbol;Acc:HGNC:24728]) HSP 1 Score: 504.212 bits (1297), Expect = 1.830e-168 Identity = 238/510 (46.67%), Postives = 339/510 (66.47%), Query Frame = 3
Query: 186 LLDKNTKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
LL K D I V +G K TPY + + +L+ S +I+KVNG WDL++PL DS +E L F+N+ A+ +W S+A +L +E + + GF D ++++++SS + ++E+ + FER+++ EI ++F ++ K ++ E++++ ++YRC LID+C+ P VR+TG++K +KI ++ S +LQRIYG+SFP+ +++ W QEEA R+HR IG+EQELFFFH+ SPGS F LPRG I N L D IRE+Y KR F+EV +P ++ ++LWE SGHW HY EN+F ++ K+ F++KPMNCP HCL+++H+ R +++P+R ADFG LHR EL+G LSGLTR+R+F QDDAHIFC +QIE+EI+ L F++ VY F F L+LSTRP+NFLG+IE+WN AE+QL+ SL FG+ W +N GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 148 LLAIYGKKGDTSNIITVRVADGQTVQGEVWKTTPYQVAAEISQELAESTVIAKVNGELWDLDRPLEGDSSLELLTFDNEEAQAVYWHSSAHILGEAMELYYGGHLCYGPPIENGFYYDMFIEDRAVSSTELSALENICKAIIKEKQPFERLEVSKEILLEMFKYNKFKCRILNEKVNTAT--TTVYRCGPLIDLCKGPHVRHTGKIKTIKIFKNSSTYWEGNPEMETLQRIYGISFPDNKMMRDWEKFQEEAKNRDHRKIGKEQELFFFHDLSPGSCFFLPRGAFIYNTLTDFIREEYHKRDFTEVLSPNMYNSKLWEASGHWQHYSENMFTFEIE-KDTFALKPMNCPGHCLMFAHRPRSWREMPIRFADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCTVEQIEEEIKGCLQFLQSVYSTFGFSFQLNLSTRPENFLGEIEMWNEAEKQLQNSLMDFGEPWKMNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTY 654 HSP 2 Score: 95.5153 bits (236), Expect = 1.330e-19 Identity = 47/91 (51.65%), Postives = 63/91 (69.23%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCIL 2047
+RPVIIHRAILGSVER +AIL E+ GKWPFWL+PRQV+V+ V +YA +V + + GF+ D DL + + +KI N + +YN IL
Sbjct: 663 KRPVIIHRAILGSVERMIAILSENYGGKWPFWLSPRQVMVIPVGPTCEKYALQVSSEFFEEGFMADVDLDHSCTLNKKIRNAQLAQYNFIL 753
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Human
Match: TARS3 (threonyl-tRNA synthetase 3 [Source:HGNC Symbol;Acc:HGNC:24728]) HSP 1 Score: 504.212 bits (1297), Expect = 1.830e-168 Identity = 238/510 (46.67%), Postives = 339/510 (66.47%), Query Frame = 3
Query: 186 LLDKNTKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
LL K D I V +G K TPY + + +L+ S +I+KVNG WDL++PL DS +E L F+N+ A+ +W S+A +L +E + + GF D ++++++SS + ++E+ + FER+++ EI ++F ++ K ++ E++++ ++YRC LID+C+ P VR+TG++K +KI ++ S +LQRIYG+SFP+ +++ W QEEA R+HR IG+EQELFFFH+ SPGS F LPRG I N L D IRE+Y KR F+EV +P ++ ++LWE SGHW HY EN+F ++ K+ F++KPMNCP HCL+++H+ R +++P+R ADFG LHR EL+G LSGLTR+R+F QDDAHIFC +QIE+EI+ L F++ VY F F L+LSTRP+NFLG+IE+WN AE+QL+ SL FG+ W +N GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 148 LLAIYGKKGDTSNIITVRVADGQTVQGEVWKTTPYQVAAEISQELAESTVIAKVNGELWDLDRPLEGDSSLELLTFDNEEAQAVYWHSSAHILGEAMELYYGGHLCYGPPIENGFYYDMFIEDRAVSSTELSALENICKAIIKEKQPFERLEVSKEILLEMFKYNKFKCRILNEKVNTAT--TTVYRCGPLIDLCKGPHVRHTGKIKTIKIFKNSSTYWEGNPEMETLQRIYGISFPDNKMMRDWEKFQEEAKNRDHRKIGKEQELFFFHDLSPGSCFFLPRGAFIYNTLTDFIREEYHKRDFTEVLSPNMYNSKLWEASGHWQHYSENMFTFEIE-KDTFALKPMNCPGHCLMFAHRPRSWREMPIRFADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCTVEQIEEEIKGCLQFLQSVYSTFGFSFQLNLSTRPENFLGEIEMWNEAEKQLQNSLMDFGEPWKMNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTY 654 HSP 2 Score: 95.5153 bits (236), Expect = 1.330e-19 Identity = 47/91 (51.65%), Postives = 63/91 (69.23%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCIL 2047
+RPVIIHRAILGSVER +AIL E+ GKWPFWL+PRQV+V+ V +YA +V + + GF+ D DL + + +KI N + +YN IL
Sbjct: 663 KRPVIIHRAILGSVERMIAILSENYGGKWPFWLSPRQVMVIPVGPTCEKYALQVSSEFFEEGFMADVDLDHSCTLNKKIRNAQLAQYNFIL 753
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Human
Match: TARS3 (threonyl-tRNA synthetase 3 [Source:HGNC Symbol;Acc:HGNC:24728]) HSP 1 Score: 505.368 bits (1300), Expect = 1.839e-168 Identity = 238/510 (46.67%), Postives = 339/510 (66.47%), Query Frame = 3
Query: 186 LLDKNTKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
LL K D I V +G K TPY + + +L+ S +I+KVNG WDL++PL DS +E L F+N+ A+ +W S+A +L +E + + GF D ++++++SS + ++E+ + FER+++ EI ++F ++ K ++ E++++ ++YRC LID+C+ P VR+TG++K +KI ++ S +LQRIYG+SFP+ +++ W QEEA R+HR IG+EQELFFFH+ SPGS F LPRG I N L D IRE+Y KR F+EV +P ++ ++LWE SGHW HY EN+F ++ K+ F++KPMNCP HCL+++H+ R +++P+R ADFG LHR EL+G LSGLTR+R+F QDDAHIFC +QIE+EI+ L F++ VY F F L+LSTRP+NFLG+IE+WN AE+QL+ SL FG+ W +N GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 148 LLAIYGKKGDTSNIITVRVADGQTVQGEVWKTTPYQVAAEISQELAESTVIAKVNGELWDLDRPLEGDSSLELLTFDNEEAQAVYWHSSAHILGEAMELYYGGHLCYGPPIENGFYYDMFIEDRAVSSTELSALENICKAIIKEKQPFERLEVSKEILLEMFKYNKFKCRILNEKVNTAT--TTVYRCGPLIDLCKGPHVRHTGKIKTIKIFKNSSTYWEGNPEMETLQRIYGISFPDNKMMRDWEKFQEEAKNRDHRKIGKEQELFFFHDLSPGSCFFLPRGAFIYNTLTDFIREEYHKRDFTEVLSPNMYNSKLWEASGHWQHYSENMFTFEIE-KDTFALKPMNCPGHCLMFAHRPRSWREMPIRFADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCTVEQIEEEIKGCLQFLQSVYSTFGFSFQLNLSTRPENFLGEIEMWNEAEKQLQNSLMDFGEPWKMNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTY 654 HSP 2 Score: 108.227 bits (269), Expect = 1.164e-23 Identity = 57/115 (49.57%), Postives = 77/115 (66.96%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR----KGHGE 2107
+RPVIIHRAILGSVER +AIL E+ GKWPFWL+PRQV+V+ V +YA +V + + GF+ D DL + + +KI N + +YN IL++G+KE V+VR K HGE
Sbjct: 663 KRPVIIHRAILGSVERMIAILSENYGGKWPFWLSPRQVMVIPVGPTCEKYALQVSSEFFEEGFMADVDLDHSCTLNKKIRNAQLAQYNFILVVGEKEKIDNAVNVRTRDNKIHGE 777
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Celegans
Match: tars-1 (Threonine--tRNA ligase, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:P52709]) HSP 1 Score: 483.797 bits (1244), Expect = 4.146e-162 Identity = 230/498 (46.18%), Postives = 326/498 (65.46%), Query Frame = 3
Query: 228 IKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVY-GILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKYF 1715
I++ +G ++ + TP+ I + + L+ + +I+KVNG WDL++P ++ +E L F++D A+ FW S+A +L +E +GF D +N++I F I+ V FER+++ E ++F ++ K+ +I E++H+ ++YRC LID+C P VR+TG++K + I ++ S + SLQR+YG+SFP+ + L W QEEA KR+HR +G+E +LFFFH SPGSAF P+G HI NKL+D IR+QYR+RGF+EV TP ++ +LWETSGHW HY E++FKI ++ KEEF +KPMNCP HCL++ H +LP R ADFG LHR E++G L+GLTR+R+F QDDAHIFCR DQI +EI++ LDF+++ Y + F F L+LSTRP+ FLG+IE W+ AE L +L G+ W LN GDGAFYGPKIDI I+DAL R QCATIQLDFQLP +F+L YF
Sbjct: 41 IQITLPDGKIHEGKTWRTTPFEIAERISKGLAEAAVIAKVNGAVWDLDRPFEGNAKLELLKFDDDEAKQVFWHSSAHVLGEAMERYCGGHLCYGPPIQEGFYYDMWHENRTICPDDFPKIDQIVKAAVKDKQKFERLEMTKEDLLEMFKYNEFKVRIITEKIHTPK--TTVYRCGPLIDLCRGPHVRHTGKVKAMAITKNSSSYWEGKADAESLQRLYGISFPDSKQLKEWQKLQEEAAKRDHRKLGKEHDLFFFHQLSPGSAFWYPKGAHIYNKLVDFIRKQYRRRGFTEVITPNMYNKKLWETSGHWQHYSEDMFKIEVE-KEEFGLKPMNCPGHCLMFGHMPHTYNELPFRFADFGVLHRNEMSGALTGLTRVRRFQQDDAHIFCRQDQISEEIKQCLDFLEYAYEKVFGFTFKLNLSTRPEGFLGNIETWDKAEADLTNALNASGRKWVLNPGDGAFYGPKIDITIQDALKRNFQCATIQLDFQLPNQFDLSYF 535 HSP 2 Score: 103.219 bits (256), Expect = 1.399e-22 Identity = 55/130 (42.31%), Postives = 82/130 (63.08%), Query Frame = 2
Query: 1766 EGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDLR-NLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+G +RPV+IHRA+LGSVER AIL ES GKWPFWL+PRQ +++V + +YAN+V +QI +AGF + + M +++ + ++N IL+IG KE ++ V+VR L +LI KF+
Sbjct: 538 KGEKQRPVMIHRAVLGSVERMTAILTESYGGKWPFWLSPRQCKIITVHESVRDYANDVKKQIFEAGFEIEYEENCGDTMNKQVRKAQLAQFNFILVIGAKEKENGTVNVRTRDNAVRGEVALDKLISKFR 667
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Celegans
Match: tars-1 (Threonine--tRNA ligase, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:P52709]) HSP 1 Score: 483.797 bits (1244), Expect = 4.146e-162 Identity = 230/498 (46.18%), Postives = 326/498 (65.46%), Query Frame = 3
Query: 228 IKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVY-GILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKYF 1715
I++ +G ++ + TP+ I + + L+ + +I+KVNG WDL++P ++ +E L F++D A+ FW S+A +L +E +GF D +N++I F I+ V FER+++ E ++F ++ K+ +I E++H+ ++YRC LID+C P VR+TG++K + I ++ S + SLQR+YG+SFP+ + L W QEEA KR+HR +G+E +LFFFH SPGSAF P+G HI NKL+D IR+QYR+RGF+EV TP ++ +LWETSGHW HY E++FKI ++ KEEF +KPMNCP HCL++ H +LP R ADFG LHR E++G L+GLTR+R+F QDDAHIFCR DQI +EI++ LDF+++ Y + F F L+LSTRP+ FLG+IE W+ AE L +L G+ W LN GDGAFYGPKIDI I+DAL R QCATIQLDFQLP +F+L YF
Sbjct: 41 IQITLPDGKIHEGKTWRTTPFEIAERISKGLAEAAVIAKVNGAVWDLDRPFEGNAKLELLKFDDDEAKQVFWHSSAHVLGEAMERYCGGHLCYGPPIQEGFYYDMWHENRTICPDDFPKIDQIVKAAVKDKQKFERLEMTKEDLLEMFKYNEFKVRIITEKIHTPK--TTVYRCGPLIDLCRGPHVRHTGKVKAMAITKNSSSYWEGKADAESLQRLYGISFPDSKQLKEWQKLQEEAAKRDHRKLGKEHDLFFFHQLSPGSAFWYPKGAHIYNKLVDFIRKQYRRRGFTEVITPNMYNKKLWETSGHWQHYSEDMFKIEVE-KEEFGLKPMNCPGHCLMFGHMPHTYNELPFRFADFGVLHRNEMSGALTGLTRVRRFQQDDAHIFCRQDQISEEIKQCLDFLEYAYEKVFGFTFKLNLSTRPEGFLGNIETWDKAEADLTNALNASGRKWVLNPGDGAFYGPKIDITIQDALKRNFQCATIQLDFQLPNQFDLSYF 535 HSP 2 Score: 103.219 bits (256), Expect = 1.399e-22 Identity = 55/130 (42.31%), Postives = 82/130 (63.08%), Query Frame = 2
Query: 1766 EGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDLR-NLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+G +RPV+IHRA+LGSVER AIL ES GKWPFWL+PRQ +++V + +YAN+V +QI +AGF + + M +++ + ++N IL+IG KE ++ V+VR L +LI KF+
Sbjct: 538 KGEKQRPVMIHRAVLGSVERMTAILTESYGGKWPFWLSPRQCKIITVHESVRDYANDVKKQIFEAGFEIEYEENCGDTMNKQVRKAQLAQFNFILVIGAKEKENGTVNVRTRDNAVRGEVALDKLISKFR 667
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Celegans
Match: tars-1 (Threonine--tRNA ligase, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:P52709]) HSP 1 Score: 483.797 bits (1244), Expect = 4.146e-162 Identity = 230/498 (46.18%), Postives = 326/498 (65.46%), Query Frame = 3
Query: 228 IKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVY-GILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKYF 1715
I++ +G ++ + TP+ I + + L+ + +I+KVNG WDL++P ++ +E L F++D A+ FW S+A +L +E +GF D +N++I F I+ V FER+++ E ++F ++ K+ +I E++H+ ++YRC LID+C P VR+TG++K + I ++ S + SLQR+YG+SFP+ + L W QEEA KR+HR +G+E +LFFFH SPGSAF P+G HI NKL+D IR+QYR+RGF+EV TP ++ +LWETSGHW HY E++FKI ++ KEEF +KPMNCP HCL++ H +LP R ADFG LHR E++G L+GLTR+R+F QDDAHIFCR DQI +EI++ LDF+++ Y + F F L+LSTRP+ FLG+IE W+ AE L +L G+ W LN GDGAFYGPKIDI I+DAL R QCATIQLDFQLP +F+L YF
Sbjct: 41 IQITLPDGKIHEGKTWRTTPFEIAERISKGLAEAAVIAKVNGAVWDLDRPFEGNAKLELLKFDDDEAKQVFWHSSAHVLGEAMERYCGGHLCYGPPIQEGFYYDMWHENRTICPDDFPKIDQIVKAAVKDKQKFERLEMTKEDLLEMFKYNEFKVRIITEKIHTPK--TTVYRCGPLIDLCRGPHVRHTGKVKAMAITKNSSSYWEGKADAESLQRLYGISFPDSKQLKEWQKLQEEAAKRDHRKLGKEHDLFFFHQLSPGSAFWYPKGAHIYNKLVDFIRKQYRRRGFTEVITPNMYNKKLWETSGHWQHYSEDMFKIEVE-KEEFGLKPMNCPGHCLMFGHMPHTYNELPFRFADFGVLHRNEMSGALTGLTRVRRFQQDDAHIFCRQDQISEEIKQCLDFLEYAYEKVFGFTFKLNLSTRPEGFLGNIETWDKAEADLTNALNASGRKWVLNPGDGAFYGPKIDITIQDALKRNFQCATIQLDFQLPNQFDLSYF 535 HSP 2 Score: 103.219 bits (256), Expect = 1.399e-22 Identity = 55/130 (42.31%), Postives = 82/130 (63.08%), Query Frame = 2
Query: 1766 EGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDLR-NLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+G +RPV+IHRA+LGSVER AIL ES GKWPFWL+PRQ +++V + +YAN+V +QI +AGF + + M +++ + ++N IL+IG KE ++ V+VR L +LI KF+
Sbjct: 538 KGEKQRPVMIHRAVLGSVERMTAILTESYGGKWPFWLSPRQCKIITVHESVRDYANDVKKQIFEAGFEIEYEENCGDTMNKQVRKAQLAQFNFILVIGAKEKENGTVNVRTRDNAVRGEVALDKLISKFR 667
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Celegans
Match: tars-1 (Threonine--tRNA ligase, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:P52709]) HSP 1 Score: 483.797 bits (1244), Expect = 1.631e-161 Identity = 230/498 (46.18%), Postives = 326/498 (65.46%), Query Frame = 3
Query: 228 IKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVY-GILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKYF 1715
I++ +G ++ + TP+ I + + L+ + +I+KVNG WDL++P ++ +E L F++D A+ FW S+A +L +E +GF D +N++I F I+ V FER+++ E ++F ++ K+ +I E++H+ ++YRC LID+C P VR+TG++K + I ++ S + SLQR+YG+SFP+ + L W QEEA KR+HR +G+E +LFFFH SPGSAF P+G HI NKL+D IR+QYR+RGF+EV TP ++ +LWETSGHW HY E++FKI ++ KEEF +KPMNCP HCL++ H +LP R ADFG LHR E++G L+GLTR+R+F QDDAHIFCR DQI +EI++ LDF+++ Y + F F L+LSTRP+ FLG+IE W+ AE L +L G+ W LN GDGAFYGPKIDI I+DAL R QCATIQLDFQLP +F+L YF
Sbjct: 82 IQITLPDGKIHEGKTWRTTPFEIAERISKGLAEAAVIAKVNGAVWDLDRPFEGNAKLELLKFDDDEAKQVFWHSSAHVLGEAMERYCGGHLCYGPPIQEGFYYDMWHENRTICPDDFPKIDQIVKAAVKDKQKFERLEMTKEDLLEMFKYNEFKVRIITEKIHTPK--TTVYRCGPLIDLCRGPHVRHTGKVKAMAITKNSSSYWEGKADAESLQRLYGISFPDSKQLKEWQKLQEEAAKRDHRKLGKEHDLFFFHQLSPGSAFWYPKGAHIYNKLVDFIRKQYRRRGFTEVITPNMYNKKLWETSGHWQHYSEDMFKIEVE-KEEFGLKPMNCPGHCLMFGHMPHTYNELPFRFADFGVLHRNEMSGALTGLTRVRRFQQDDAHIFCRQDQISEEIKQCLDFLEYAYEKVFGFTFKLNLSTRPEGFLGNIETWDKAEADLTNALNASGRKWVLNPGDGAFYGPKIDITIQDALKRNFQCATIQLDFQLPNQFDLSYF 576 HSP 2 Score: 103.219 bits (256), Expect = 1.650e-22 Identity = 55/130 (42.31%), Postives = 82/130 (63.08%), Query Frame = 2
Query: 1766 EGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDLR-NLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+G +RPV+IHRA+LGSVER AIL ES GKWPFWL+PRQ +++V + +YAN+V +QI +AGF + + M +++ + ++N IL+IG KE ++ V+VR L +LI KF+
Sbjct: 579 KGEKQRPVMIHRAVLGSVERMTAILTESYGGKWPFWLSPRQCKIITVHESVRDYANDVKKQIFEAGFEIEYEENCGDTMNKQVRKAQLAQFNFILVIGAKEKENGTVNVRTRDNAVRGEVALDKLISKFR 708
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Fly
Match: ThrRS (gene:FBgn0027081 transcript:FBtr0080333) HSP 1 Score: 484.567 bits (1246), Expect = 6.905e-166 Identity = 230/495 (46.46%), Postives = 326/495 (65.86%), Query Frame = 3
Query: 228 IKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
IKV +G + + TPY + + + L+++ +ISKVNG WDL++ L + ++ L F++ A+ FW S+A ++ +E ++ + GF D L+ + IS+ + ++E V +FER+++ ++F ++ K+ ++ + ++ ++Y+C LID+C P VR+TG++K LKI ++ S + +LQR+YG+SFP+ + L W QEEA KR+HR IG EQELFFFH SPGS F PRG HI N L+ I+ +YRKRGF EV +P I+ +LW TSGHW HY EN+F + KE+F++KPMNCP HCLI+ ++ R ++LPLR+ADFG LHR EL+G L+GLTR+R+F QDDAHIFC P+QI+ E++ L+F+K+VY I F F L LSTRPDN+LG++E WN AE+ L +SL +FG W N GDGAFYGPKIDI I DAL R HQCATIQLDFQLP+RFNL Y
Sbjct: 52 IKVTLPDGKQVDATSWETTPYEVARGISQGLADNTVISKVNGEVWDLDRVLEGNCTLQLLKFDDPEAQAVFWHSSAHIMGEAMERIYGGHLCYGPPIENGFYYDMHLEGEGISTNDYGAMEGLVKQIVKEKQNFERLEMKKSDLLEMFKYNEFKVRILNEKVTTDR-TTVYKCGSLIDLCRGPHVRHTGKVKALKITKNSSTYWEGKADAETLQRVYGISFPDPKQLKEWEKLQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLMGFIKAEYRKRGFQEVISPNIYNAKLWMTSGHWQHYAENMFSFEAE-KEKFALKPMNCPGHCLIFDNRNRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAPEQIKSEMKGCLEFLKYVYTIFGFSFQLVLSTRPDNYLGELEQWNDAEKALAESLNEFGMPWKENPGDGAFYGPKIDITIMDALKRAHQCATIQLDFQLPIRFNLSY 544 HSP 2 Score: 120.939 bits (302), Expect = 6.905e-166 Identity = 62/133 (46.62%), Postives = 86/133 (64.66%), Query Frame = 2
Query: 1757 LRNEGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+ ++G +RPVIIHRAILGSVER +AIL E+ +GKWPFWL+PRQV+VV V + +YA V Q+ DAGF+ + D M +KI N + ++N IL++G KE S V+VR + H + ELI K +
Sbjct: 545 IADDGEKKRPVIIHRAILGSVERMIAILTENFAGKWPFWLSPRQVMVVPVGPAYDQYAQSVRDQLHDAGFMSEADCDAGDTMNKKIRNAQLAQFNFILVVGDKERSSNTVNVRTRDNKVHGEVSVAELITKLQ 677
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Fly
Match: ThrRS (gene:FBgn0027081 transcript:FBtr0080331) HSP 1 Score: 484.567 bits (1246), Expect = 6.905e-166 Identity = 230/495 (46.46%), Postives = 326/495 (65.86%), Query Frame = 3
Query: 228 IKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
IKV +G + + TPY + + + L+++ +ISKVNG WDL++ L + ++ L F++ A+ FW S+A ++ +E ++ + GF D L+ + IS+ + ++E V +FER+++ ++F ++ K+ ++ + ++ ++Y+C LID+C P VR+TG++K LKI ++ S + +LQR+YG+SFP+ + L W QEEA KR+HR IG EQELFFFH SPGS F PRG HI N L+ I+ +YRKRGF EV +P I+ +LW TSGHW HY EN+F + KE+F++KPMNCP HCLI+ ++ R ++LPLR+ADFG LHR EL+G L+GLTR+R+F QDDAHIFC P+QI+ E++ L+F+K+VY I F F L LSTRPDN+LG++E WN AE+ L +SL +FG W N GDGAFYGPKIDI I DAL R HQCATIQLDFQLP+RFNL Y
Sbjct: 52 IKVTLPDGKQVDATSWETTPYEVARGISQGLADNTVISKVNGEVWDLDRVLEGNCTLQLLKFDDPEAQAVFWHSSAHIMGEAMERIYGGHLCYGPPIENGFYYDMHLEGEGISTNDYGAMEGLVKQIVKEKQNFERLEMKKSDLLEMFKYNEFKVRILNEKVTTDR-TTVYKCGSLIDLCRGPHVRHTGKVKALKITKNSSTYWEGKADAETLQRVYGISFPDPKQLKEWEKLQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLMGFIKAEYRKRGFQEVISPNIYNAKLWMTSGHWQHYAENMFSFEAE-KEKFALKPMNCPGHCLIFDNRNRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAPEQIKSEMKGCLEFLKYVYTIFGFSFQLVLSTRPDNYLGELEQWNDAEKALAESLNEFGMPWKENPGDGAFYGPKIDITIMDALKRAHQCATIQLDFQLPIRFNLSY 544 HSP 2 Score: 120.939 bits (302), Expect = 6.905e-166 Identity = 62/133 (46.62%), Postives = 86/133 (64.66%), Query Frame = 2
Query: 1757 LRNEGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+ ++G +RPVIIHRAILGSVER +AIL E+ +GKWPFWL+PRQV+VV V + +YA V Q+ DAGF+ + D M +KI N + ++N IL++G KE S V+VR + H + ELI K +
Sbjct: 545 IADDGEKKRPVIIHRAILGSVERMIAILTENFAGKWPFWLSPRQVMVVPVGPAYDQYAQSVRDQLHDAGFMSEADCDAGDTMNKKIRNAQLAQFNFILVVGDKERSSNTVNVRTRDNKVHGEVSVAELITKLQ 677
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Fly
Match: ThrRS (gene:FBgn0027081 transcript:FBtr0080332) HSP 1 Score: 483.797 bits (1244), Expect = 8.154e-166 Identity = 230/495 (46.46%), Postives = 326/495 (65.86%), Query Frame = 3
Query: 228 IKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
IKV +G + + TPY + + + L+++ +ISKVNG WDL++ L + ++ L F++ A+ FW S+A ++ +E ++ + GF D L+ + IS+ + ++E V +FER+++ ++F ++ K+ ++ + ++ ++Y+C LID+C P VR+TG++K LKI ++ S + +LQR+YG+SFP+ + L W QEEA KR+HR IG EQELFFFH SPGS F PRG HI N L+ I+ +YRKRGF EV +P I+ +LW TSGHW HY EN+F + KE+F++KPMNCP HCLI+ ++ R ++LPLR+ADFG LHR EL+G L+GLTR+R+F QDDAHIFC P+QI+ E++ L+F+K+VY I F F L LSTRPDN+LG++E WN AE+ L +SL +FG W N GDGAFYGPKIDI I DAL R HQCATIQLDFQLP+RFNL Y
Sbjct: 109 IKVTLPDGKQVDATSWETTPYEVARGISQGLADNTVISKVNGEVWDLDRVLEGNCTLQLLKFDDPEAQAVFWHSSAHIMGEAMERIYGGHLCYGPPIENGFYYDMHLEGEGISTNDYGAMEGLVKQIVKEKQNFERLEMKKSDLLEMFKYNEFKVRILNEKVTTDR-TTVYKCGSLIDLCRGPHVRHTGKVKALKITKNSSTYWEGKADAETLQRVYGISFPDPKQLKEWEKLQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLMGFIKAEYRKRGFQEVISPNIYNAKLWMTSGHWQHYAENMFSFEAE-KEKFALKPMNCPGHCLIFDNRNRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAPEQIKSEMKGCLEFLKYVYTIFGFSFQLVLSTRPDNYLGELEQWNDAEKALAESLNEFGMPWKENPGDGAFYGPKIDITIMDALKRAHQCATIQLDFQLPIRFNLSY 601 HSP 2 Score: 121.324 bits (303), Expect = 8.154e-166 Identity = 62/133 (46.62%), Postives = 86/133 (64.66%), Query Frame = 2
Query: 1757 LRNEGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+ ++G +RPVIIHRAILGSVER +AIL E+ +GKWPFWL+PRQV+VV V + +YA V Q+ DAGF+ + D M +KI N + ++N IL++G KE S V+VR + H + ELI K +
Sbjct: 602 IADDGEKKRPVIIHRAILGSVERMIAILTENFAGKWPFWLSPRQVMVVPVGPAYDQYAQSVRDQLHDAGFMSEADCDAGDTMNKKIRNAQLAQFNFILVVGDKERSSNTVNVRTRDNKVHGEVSVAELITKLQ 734
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Fly
Match: ThrRS (gene:FBgn0027081 transcript:FBtr0346436) HSP 1 Score: 483.797 bits (1244), Expect = 8.154e-166 Identity = 230/495 (46.46%), Postives = 326/495 (65.86%), Query Frame = 3
Query: 228 IKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
IKV +G + + TPY + + + L+++ +ISKVNG WDL++ L + ++ L F++ A+ FW S+A ++ +E ++ + GF D L+ + IS+ + ++E V +FER+++ ++F ++ K+ ++ + ++ ++Y+C LID+C P VR+TG++K LKI ++ S + +LQR+YG+SFP+ + L W QEEA KR+HR IG EQELFFFH SPGS F PRG HI N L+ I+ +YRKRGF EV +P I+ +LW TSGHW HY EN+F + KE+F++KPMNCP HCLI+ ++ R ++LPLR+ADFG LHR EL+G L+GLTR+R+F QDDAHIFC P+QI+ E++ L+F+K+VY I F F L LSTRPDN+LG++E WN AE+ L +SL +FG W N GDGAFYGPKIDI I DAL R HQCATIQLDFQLP+RFNL Y
Sbjct: 109 IKVTLPDGKQVDATSWETTPYEVARGISQGLADNTVISKVNGEVWDLDRVLEGNCTLQLLKFDDPEAQAVFWHSSAHIMGEAMERIYGGHLCYGPPIENGFYYDMHLEGEGISTNDYGAMEGLVKQIVKEKQNFERLEMKKSDLLEMFKYNEFKVRILNEKVTTDR-TTVYKCGSLIDLCRGPHVRHTGKVKALKITKNSSTYWEGKADAETLQRVYGISFPDPKQLKEWEKLQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLMGFIKAEYRKRGFQEVISPNIYNAKLWMTSGHWQHYAENMFSFEAE-KEKFALKPMNCPGHCLIFDNRNRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAPEQIKSEMKGCLEFLKYVYTIFGFSFQLVLSTRPDNYLGELEQWNDAEKALAESLNEFGMPWKENPGDGAFYGPKIDITIMDALKRAHQCATIQLDFQLPIRFNLSY 601 HSP 2 Score: 121.324 bits (303), Expect = 8.154e-166 Identity = 62/133 (46.62%), Postives = 86/133 (64.66%), Query Frame = 2
Query: 1757 LRNEGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+ ++G +RPVIIHRAILGSVER +AIL E+ +GKWPFWL+PRQV+VV V + +YA V Q+ DAGF+ + D M +KI N + ++N IL++G KE S V+VR + H + ELI K +
Sbjct: 602 IADDGEKKRPVIIHRAILGSVERMIAILTENFAGKWPFWLSPRQVMVVPVGPAYDQYAQSVRDQLHDAGFMSEADCDAGDTMNKKIRNAQLAQFNFILVVGDKERSSNTVNVRTRDNKVHGEVSVAELITKLQ 734
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Fly
Match: mRpL39 (gene:FBgn0036462 transcript:FBtr0075672) HSP 1 Score: 71.633 bits (174), Expect = 4.306e-13 Identity = 56/235 (23.83%), Postives = 109/235 (46.38%), Query Frame = 3
Query: 234 VMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNG-LFWDLNKPLVNDSDIEFLLF---ENDIARDAFWRSTAFLLNGILEHLFSSET-YRSHS------NDKGFICDFIL--KNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSE-NKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPN 896
+ +NG+++ TP+ Q+ + ++ ++G + WD+++PL ++ L F E + AFWR+ +F+L L F E + HS F+ D +L +N + +++ + + A ER+D+ ++A+++F S K + + + + N ++LYR D IDI P+V +T L K V S + ++ + RI GV+ P+
Sbjct: 70 TLVMNGNIS-------TPFNCAQHLSEGHCKRSALALIDGSVPWDMHRPLQESCTLQLLNFHVSEPHVVNKAFWRTCSFMLGAALNRAFKPEANLQLHSFPGPNIKSGSFVHDIVLQTQNWEPGKEEMRALSAEMVKLAAQDLRIERLDVQQDLAQEMFKDSKYKSEQLPSISQQTNGRVTLYRLGDHIDISRGPMVASTSFLG--KCVISAAHKVAEEGPSGAFYRIQGVALPS 295
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Zebrafish
Match: tars1 (threonyl-tRNA synthetase 1 [Source:ZFIN;Acc:ZDB-GENE-041010-218]) HSP 1 Score: 496.508 bits (1277), Expect = 2.896e-166 Identity = 238/504 (47.22%), Postives = 337/504 (66.87%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + + K IK+ +G + + K TPY + + L+++ +ISKVN WDL++PL D + FL F+++ A+ +W S+A ++ +E ++ + GF D L N+ +SS F +E+ + FER+++ E ++F ++ K ++ E++ + ++YRC LID+C P VR+TG++K +KI ++ S + SLQRIYG+SFP+ ++L W QEEA R+HR +G EQ+LFFFH+ SPGS F LP+G +I N LI+ IR +YRKRGF EV TP I+ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC DQIE EI+ LDF++ VY + F F L+LSTRP+ FLG+ E+W+ AE+QL+ SL FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL +
Sbjct: 70 KAAKESKPIKITLPDGKVVDGESWKTTPYQVACGISQGLADNTVISKVNNGVWDLDRPLEEDCSLVFLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLDNEGVSSNDFPCLENLCKKIIKEKQPFERLEVKKETLLEMFKYNKFKCRILNEKVTTPT--TTVYRCGPLIDLCRGPHVRHTGKIKAMKIHKNSSTYWEGKADMESLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKLGREQDLFFFHDLSPGSCFFLPKGAYIYNTLIEFIRSEYRKRGFQEVVTPNIYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCSMDQIESEIKGCLDFLRTVYDVFGFTFKLNLSTRPEKFLGEPEVWDQAEKQLENSLNDFGEKWVLNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTF 570 HSP 2 Score: 108.997 bits (271), Expect = 4.030e-24 Identity = 56/126 (44.44%), Postives = 78/126 (61.90%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AIL E+ GKWP WL+PRQV+VV + EYA V ++ G + D DL + +KI N + +YN IL++G+KE S V+VR + H L + +++ K
Sbjct: 579 KRPVIIHRAILGSVERMIAILTENYGGKWPLWLSPRQVMVVPIGPTCDEYAQRVQKEFHKVGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKSSDTVNVRTRDNKVHGERSLTDCMERLK 704
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Zebrafish
Match: tars1 (threonyl-tRNA synthetase 1 [Source:ZFIN;Acc:ZDB-GENE-041010-218]) HSP 1 Score: 449.514 bits (1155), Expect = 9.621e-152 Identity = 224/504 (44.44%), Postives = 326/504 (64.68%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + + K IK+ +G + + K TPY + + L+++ +ISKVN WDL++PL D + FL F+++ A+ +W S+A ++ +E ++ + GF D L N+ +SS F +E+ + FER+++ E ++F ++ K ++ E++ + ++YRC LID+C P VR+TG++K +KI ++ S + SLQRIYG+SFP+ ++L W QEEA R+HR +G EQ+LFFFH+ SPGS F LP+G +I N LI+ IR +YRKRGF EV TP I+ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC DQIE EI+ LDF++ VY + F F L+LSTRP+ FLG+ E+W+ AE+ ++ S +LN G + + IDI IKDA+ R HQCATIQLDFQLP+RFNL +
Sbjct: 52 KAAKESKPIKITLPDGKVVDGESWKTTPYQVACGISQGLADNTVISKVNNGVWDLDRPLEEDCSLVFLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLDNEGVSSNDFPCLENLCKKIIKEKQPFERLEVKKETLLEMFKYNKFKCRILNEKVTTPT--TTVYRCGPLIDLCRGPHVRHTGKIKAMKIHKNSSTYWEGKADMESLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKLGREQDLFFFHDLSPGSCFFLPKGAYIYNTLIEFIRSEYRKRGFQEVVTPNIYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCSMDQIESEIKGCLDFLRTVYDVFGFTFKLNLSTRPEKFLGEPEVWDQAEKVKFTTV-----SITLNFGADSTF---IDIQIKDAIGRYHQCATIQLDFQLPIRFNLTF 544 HSP 2 Score: 108.997 bits (271), Expect = 9.621e-152 Identity = 56/126 (44.44%), Postives = 78/126 (61.90%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AIL E+ GKWP WL+PRQV+VV + EYA V ++ G + D DL + +KI N + +YN IL++G+KE S V+VR + H L + +++ K
Sbjct: 553 KRPVIIHRAILGSVERMIAILTENYGGKWPLWLSPRQVMVVPIGPTCDEYAQRVQKEFHKVGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKSSDTVNVRTRDNKVHGERSLTDCMERLK 678
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Zebrafish
Match: tars1 (threonyl-tRNA synthetase 1 [Source:ZFIN;Acc:ZDB-GENE-041010-218]) HSP 1 Score: 440.654 bits (1132), Expect = 4.010e-149 Identity = 219/504 (43.45%), Postives = 317/504 (62.90%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + + K IK+ +G + + K TPY + + L+++ +ISKVN WDL++PL D + FL F+++ A+ +W S+A ++ +E ++ + GF D L N+ +SS F +E+ + FER+++ E ++F ++ K ++ E++ + ++YRC LID+C P VRNTG++K +KI ++ S + SLQRIYG+SFP+ ++L W QEEA R+HR +G EQ+LFFFH+ SPGS F LP+G +I N LI+ IR +YRKRGF EV TP I+ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC DQIE EI+ LDF++ VY + F F L+LSTRP+ FLG+ E++ ++L + IDI IKDA+ R HQCATIQLDFQLP+RFNL +
Sbjct: 52 KAAKESKPIKITLPDGKVVDGESWKTTPYQVACGISQGLADNTVISKVNNGVWDLDRPLEEDCSLVFLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLDNEGVSSNDFPCLENLCKKIIKEKQPFERLEVKKETLLEMFKYNKFKCRILNEKVTTPT--TTVYRCGPLIDLCRGPHVRNTGKIKAMKIHKNSSTYWEGKADMESLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKLGREQDLFFFHDLSPGSCFFLPKGAYIYNTLIEFIRSEYRKRGFQEVVTPNIYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCSMDQIESEIKGCLDFLRTVYDVFGFTFKLNLSTRPEKFLGEPEVYKQMNKELPEHF--------------------IDIQIKDAIGRYHQCATIQLDFQLPIRFNLTF 532 HSP 2 Score: 108.997 bits (271), Expect = 4.010e-149 Identity = 56/126 (44.44%), Postives = 78/126 (61.90%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AIL E+ GKWP WL+PRQV+VV + EYA V ++ G + D DL + +KI N + +YN IL++G+KE S V+VR + H L + +++ K
Sbjct: 541 KRPVIIHRAILGSVERMIAILTENYGGKWPLWLSPRQVMVVPIGPTCDEYAQRVQKEFHKVGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKSSDTVNVRTRDNKVHGERSLTDCMERLK 666
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Zebrafish
Match: tars1 (threonyl-tRNA synthetase 1 [Source:ZFIN;Acc:ZDB-GENE-041010-218]) HSP 1 Score: 449.129 bits (1154), Expect = 3.475e-148 Identity = 224/504 (44.44%), Postives = 326/504 (64.68%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + + K IK+ +G + + K TPY + + L+++ +ISKVN WDL++PL D + FL F+++ A+ +W S+A ++ +E ++ + GF D L N+ +SS F +E+ + FER+++ E ++F ++ K ++ E++ + ++YRC LID+C P VR+TG++K +KI ++ S + SLQRIYG+SFP+ ++L W QEEA R+HR +G EQ+LFFFH+ SPGS F LP+G +I N LI+ IR +YRKRGF EV TP I+ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC DQIE EI+ LDF++ VY + F F L+LSTRP+ FLG+ E+W+ AE+ ++ S +LN G + + IDI IKDA+ R HQCATIQLDFQLP+RFNL +
Sbjct: 52 KAAKESKPIKITLPDGKVVDGESWKTTPYQVACGISQGLADNTVISKVNNGVWDLDRPLEEDCSLVFLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLDNEGVSSNDFPCLENLCKKIIKEKQPFERLEVKKETLLEMFKYNKFKCRILNEKVTTPT--TTVYRCGPLIDLCRGPHVRHTGKIKAMKIHKNSSTYWEGKADMESLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKLGREQDLFFFHDLSPGSCFFLPKGAYIYNTLIEFIRSEYRKRGFQEVVTPNIYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCSMDQIESEIKGCLDFLRTVYDVFGFTFKLNLSTRPEKFLGEPEVWDQAEKVKFTTV-----SITLNFGADSTF---IDIQIKDAIGRYHQCATIQLDFQLPIRFNLTF 544 HSP 2 Score: 94.3597 bits (233), Expect = 1.561e-19 Identity = 46/93 (49.46%), Postives = 61/93 (65.59%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILII 2053
+RPVIIHRAILGSVER +AIL E+ GKWP WL+PRQV+VV + EYA V ++ G + D DL + +KI N + +YN IL++
Sbjct: 553 KRPVIIHRAILGSVERMIAILTENYGGKWPLWLSPRQVMVVPIGPTCDEYAQRVQKEFHKVGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVL 645
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Zebrafish
Match: tars1 (threonyl-tRNA synthetase 1 [Source:ZFIN;Acc:ZDB-GENE-041010-218]) HSP 1 Score: 440.654 bits (1132), Expect = 4.774e-145 Identity = 219/504 (43.45%), Postives = 317/504 (62.90%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + + K IK+ +G + + K TPY + + L+++ +ISKVN WDL++PL D + FL F+++ A+ +W S+A ++ +E ++ + GF D L N+ +SS F +E+ + FER+++ E ++F ++ K ++ E++ + ++YRC LID+C P VRNTG++K +KI ++ S + SLQRIYG+SFP+ ++L W QEEA R+HR +G EQ+LFFFH+ SPGS F LP+G +I N LI+ IR +YRKRGF EV TP I+ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC DQIE EI+ LDF++ VY + F F L+LSTRP+ FLG+ E++ ++L + IDI IKDA+ R HQCATIQLDFQLP+RFNL +
Sbjct: 52 KAAKESKPIKITLPDGKVVDGESWKTTPYQVACGISQGLADNTVISKVNNGVWDLDRPLEEDCSLVFLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLDNEGVSSNDFPCLENLCKKIIKEKQPFERLEVKKETLLEMFKYNKFKCRILNEKVTTPT--TTVYRCGPLIDLCRGPHVRNTGKIKAMKIHKNSSTYWEGKADMESLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKLGREQDLFFFHDLSPGSCFFLPKGAYIYNTLIEFIRSEYRKRGFQEVVTPNIYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCSMDQIESEIKGCLDFLRTVYDVFGFTFKLNLSTRPEKFLGEPEVYKQMNKELPEHF--------------------IDIQIKDAIGRYHQCATIQLDFQLPIRFNLTF 532 HSP 2 Score: 94.3597 bits (233), Expect = 1.666e-19 Identity = 46/93 (49.46%), Postives = 61/93 (65.59%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILII 2053
+RPVIIHRAILGSVER +AIL E+ GKWP WL+PRQV+VV + EYA V ++ G + D DL + +KI N + +YN IL++
Sbjct: 541 KRPVIIHRAILGSVERMIAILTENYGGKWPLWLSPRQVMVVPIGPTCDEYAQRVQKEFHKVGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVL 633
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Xenopus
Match: tars (threonyl-tRNA synthetase 1 [Source:NCBI gene;Acc:407892]) HSP 1 Score: 505.753 bits (1301), Expect = 3.657e-170 Identity = 238/503 (47.32%), Postives = 337/503 (67.00%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + + K IKV +G + K TPY I + L+++ +++KVNG WDL++PL D + L F+++ A+ +W S+A ++ +E ++ + GF D L++ +SS F S+E+ + FER+++ E ++F ++ K ++ + ++YRC LID+C P VR+TG++K LK+ ++ S + +LQR+YG+SFP+ ++L W QEEA R+HR IG EQELFFFH SPGS F LP+G +I N+LI IR++YRKRGF EV TP ++ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLR+ADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE+EI+ LDF++ VY + F F L+LSTRP+ FLGDIE+WN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 38 KAAKESKAIKVTLPDGKQVDAESWKSTPYQIACGISQGLADNTVVAKVNGSVWDLDRPLEEDCTLALLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEDGGVSSNDFTSLENLCKKIMKEKQQFERLEVTKETLLEMFKYNKFKCRILNE-KVDTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKVHKNSSTYWEGKADMETLQRVYGISFPDPKMLKEWEKFQEEAKNRDHRKIGREQELFFFHELSPGSCFFLPKGAYIYNQLIAFIRQEYRKRGFQEVVTPNVYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEEEIKSCLDFLRAVYNVFGFTFKLNLSTRPEKFLGDIEVWNQAEKQLENSLNEFGEKWVLNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 538 HSP 2 Score: 106.301 bits (264), Expect = 3.646e-23 Identity = 58/125 (46.40%), Postives = 79/125 (63.20%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
+RPVIIHRAILGSVER +AIL E+ GKWP WL+P+QV+VV V EYA +V +Q AG + D DL + +KI N + +YN IL+IG+KE S V++R + H + E I++
Sbjct: 547 KRPVIIHRAILGSVERMIAILTENFGGKWPLWLSPQQVMVVPVGPTCDEYAQQV-KQFHRAGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVIGEKEKASGTVNIRTRDNKVHGERTIAETIERL 670
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Xenopus
Match: tars (threonyl-tRNA synthetase 1 [Source:NCBI gene;Acc:407892]) HSP 1 Score: 505.368 bits (1300), Expect = 4.734e-170 Identity = 242/509 (47.54%), Postives = 339/509 (66.60%), Query Frame = 3
Query: 186 LLDKNTKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
L DK K S K IKV +G + K TPY I + L+++ +++KVNG WDL++PL D + L F+++ A+ +W S+A ++ +E ++ + GF D L++ +SS F S+E+ + FER+++ E ++F ++ K ++ + ++YRC LID+C P VR+TG++K LK+ ++ S + +LQR+YG+SFP+ ++L W QEEA R+HR IG EQELFFFH SPGS F LP+G +I N+LI IR++YRKRGF EV TP ++ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLR+ADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE+EI+ LDF++ VY + F F L+LSTRP+ FLGDIE+WN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 35 LADKAAKES---KAIKVTLPDGKQVDAESWKSTPYQIACGISQGLADNTVVAKVNGSVWDLDRPLEEDCTLALLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEDGGVSSNDFTSLENLCKKIMKEKQQFERLEVTKETLLEMFKYNKFKCRILNE-KVDTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKVHKNSSTYWEGKADMETLQRVYGISFPDPKMLKEWEKFQEEAKNRDHRKIGREQELFFFHELSPGSCFFLPKGAYIYNQLIAFIRQEYRKRGFQEVVTPNVYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEEEIKSCLDFLRAVYNVFGFTFKLNLSTRPEKFLGDIEVWNQAEKQLENSLNEFGEKWVLNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 538 HSP 2 Score: 109.768 bits (273), Expect = 3.517e-24 Identity = 58/125 (46.40%), Postives = 79/125 (63.20%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
+RPVIIHRAILGSVER +AIL E+ GKWP WL+P+QV+VV V EYA +V +Q AG + D DL + +KI N + +YN IL+IG+KE S V++R + H + E I++
Sbjct: 547 KRPVIIHRAILGSVERMIAILTENFGGKWPLWLSPQQVMVVPVGPTCDEYAQQVREQFHRAGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVIGEKEKASGTVNIRTRDNKVHGERTIAETIERL 671
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Xenopus
Match: tars (threonyl-tRNA synthetase 1 [Source:NCBI gene;Acc:407892]) HSP 1 Score: 506.138 bits (1302), Expect = 7.435e-170 Identity = 238/503 (47.32%), Postives = 337/503 (67.00%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + + K IKV +G + K TPY I + L+++ +++KVNG WDL++PL D + L F+++ A+ +W S+A ++ +E ++ + GF D L++ +SS F S+E+ + FER+++ E ++F ++ K ++ + ++YRC LID+C P VR+TG++K LK+ ++ S + +LQR+YG+SFP+ ++L W QEEA R+HR IG EQELFFFH SPGS F LP+G +I N+LI IR++YRKRGF EV TP ++ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLR+ADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE+EI+ LDF++ VY + F F L+LSTRP+ FLGDIE+WN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 73 KAAKESKAIKVTLPDGKQVDAESWKSTPYQIACGISQGLADNTVVAKVNGSVWDLDRPLEEDCTLALLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEDGGVSSNDFTSLENLCKKIMKEKQQFERLEVTKETLLEMFKYNKFKCRILNE-KVDTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKVHKNSSTYWEGKADMETLQRVYGISFPDPKMLKEWEKFQEEAKNRDHRKIGREQELFFFHELSPGSCFFLPKGAYIYNQLIAFIRQEYRKRGFQEVVTPNVYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEEEIKSCLDFLRAVYNVFGFTFKLNLSTRPEKFLGDIEVWNQAEKQLENSLNEFGEKWVLNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 573 HSP 2 Score: 109.768 bits (273), Expect = 3.188e-24 Identity = 58/125 (46.40%), Postives = 79/125 (63.20%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
+RPVIIHRAILGSVER +AIL E+ GKWP WL+P+QV+VV V EYA +V +Q AG + D DL + +KI N + +YN IL+IG+KE S V++R + H + E I++
Sbjct: 582 KRPVIIHRAILGSVERMIAILTENFGGKWPLWLSPQQVMVVPVGPTCDEYAQQVREQFHRAGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVIGEKEKASGTVNIRTRDNKVHGERTIAETIERL 706
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Xenopus
Match: tars (threonyl-tRNA synthetase 1 [Source:NCBI gene;Acc:407892]) HSP 1 Score: 505.753 bits (1301), Expect = 1.311e-169 Identity = 238/503 (47.32%), Postives = 337/503 (67.00%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + + K IKV +G + K TPY I + L+++ +++KVNG WDL++PL D + L F+++ A+ +W S+A ++ +E ++ + GF D L++ +SS F S+E+ + FER+++ E ++F ++ K ++ + ++YRC LID+C P VR+TG++K LK+ ++ S + +LQR+YG+SFP+ ++L W QEEA R+HR IG EQELFFFH SPGS F LP+G +I N+LI IR++YRKRGF EV TP ++ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLR+ADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE+EI+ LDF++ VY + F F L+LSTRP+ FLGDIE+WN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 69 KAAKESKAIKVTLPDGKQVDAESWKSTPYQIACGISQGLADNTVVAKVNGSVWDLDRPLEEDCTLALLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEDGGVSSNDFTSLENLCKKIMKEKQQFERLEVTKETLLEMFKYNKFKCRILNE-KVDTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKVHKNSSTYWEGKADMETLQRVYGISFPDPKMLKEWEKFQEEAKNRDHRKIGREQELFFFHELSPGSCFFLPKGAYIYNQLIAFIRQEYRKRGFQEVVTPNVYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEEEIKSCLDFLRAVYNVFGFTFKLNLSTRPEKFLGDIEVWNQAEKQLENSLNEFGEKWVLNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 569 HSP 2 Score: 83.5741 bits (205), Expect = 6.132e-16 Identity = 52/132 (39.39%), Postives = 73/132 (55.30%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKW-----PFWLNPRQVLVVSVS--DKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
+RPVIIHRAILGSVER +AIL E+ GKW F PRQV V + + + +V +Q AG + D DL + +KI N + +YN IL+IG+KE S V++R + H + E I++
Sbjct: 578 KRPVIIHRAILGSVERMIAILTENFGGKWGKIERAFISAPRQVTVTATTPLQSVLGNYTQVREQFHRAGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVIGEKEKASGTVNIRTRDNKVHGERTIAETIERL 709
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Xenopus
Match: tars (threonyl-tRNA synthetase 1 [Source:NCBI gene;Acc:407892]) HSP 1 Score: 504.597 bits (1298), Expect = 2.485e-169 Identity = 238/503 (47.32%), Postives = 337/503 (67.00%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + + K IKV +G + K TPY I + L+++ +++KVNG WDL++PL D + L F+++ A+ +W S+A ++ +E ++ + GF D L++ +SS F S+E+ + FER+++ E ++F ++ K ++ + ++YRC LID+C P VR+TG++K LK+ ++ S + +LQR+YG+SFP+ ++L W QEEA R+HR IG EQELFFFH SPGS F LP+G +I N+LI IR++YRKRGF EV TP ++ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLR+ADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE+EI+ LDF++ VY + F F L+LSTRP+ FLGDIE+WN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 67 KAAKESKAIKVTLPDGKQVDAESWKSTPYQIACGISQGLADNTVVAKVNGSVWDLDRPLEEDCTLALLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEDGGVSSNDFTSLENLCKKIMKEKQQFERLEVTKETLLEMFKYNKFKCRILNE-KVDTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKVHKNSSTYWEGKADMETLQRVYGISFPDPKMLKEWEKFQEEAKNRDHRKIGREQELFFFHELSPGSCFFLPKGAYIYNQLIAFIRQEYRKRGFQEVVTPNVYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEEEIKSCLDFLRAVYNVFGFTFKLNLSTRPEKFLGDIEVWNQAEKQLENSLNEFGEKWVLNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 567 HSP 2 Score: 109.768 bits (273), Expect = 3.618e-24 Identity = 58/125 (46.40%), Postives = 79/125 (63.20%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
+RPVIIHRAILGSVER +AIL E+ GKWP WL+P+QV+VV V EYA +V +Q AG + D DL + +KI N + +YN IL+IG+KE S V++R + H + E I++
Sbjct: 576 KRPVIIHRAILGSVERMIAILTENFGGKWPLWLSPQQVMVVPVGPTCDEYAQQVREQFHRAGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVIGEKEKASGTVNIRTRDNKVHGERTIAETIERL 700
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Mouse
Match: Tars (threonyl-tRNA synthetase [Source:MGI Symbol;Acc:MGI:106314]) HSP 1 Score: 508.064 bits (1307), Expect = 1.519e-172 Identity = 245/504 (48.61%), Postives = 337/504 (66.87%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + D K IKV +G + K TPY I + L+++ +++KVN + WDL++PL D +E L FE++ A+ +W S+A ++ +E ++ + GF D L+ +SS F S+E + FER+++ E ++F ++ K ++ E++++ ++YRC LID+C P VR+TG++K LKI ++ S + +LQRIYG+SFP+ +LL W QEEA R+HR IG +QEL+FFH SPGS F LP+G +I N L++ IR +YRKRGF EV TP IF + LW TSGHW HY EN+F ++ KE+F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE EI+ LDF++ VY + F F L+LSTRP+ FLGDIEIWN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 74 KAAKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVVAKVNKVVWDLDRPLETDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLETLCKKIIKEKQTFERLEVKKETLLEMFKYNKFKCRILNEKVNTPT--TTVYRCGPLIDLCRGPHVRHTGKIKTLKIHKNSSTYWEGKADMETLQRIYGISFPDPKLLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNTLMEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVE-KEQFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEIWNQAEKQLENSLNEFGEKWELNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 574 HSP 2 Score: 119.783 bits (299), Expect = 1.519e-172 Identity = 60/126 (47.62%), Postives = 84/126 (66.67%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVI+HRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V EYA +V QQ DA F+ DTDL + +KI N + +YN IL++G+KE S V++R + H ++E +++ +
Sbjct: 583 KRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADTDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKASGTVNIRTRDNKVHGERTVEETVRRLQ 708
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Mouse
Match: Tarsl2 (threonyl-tRNA synthetase-like 2 [Source:MGI Symbol;Acc:MGI:2444486]) HSP 1 Score: 500.745 bits (1288), Expect = 5.829e-167 Identity = 238/533 (44.65%), Postives = 343/533 (64.35%), Query Frame = 3
Query: 114 FLKRSLSENELLKVQQIKQFLWKDLLDKNTKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
F+K L E LK LL + + + I V G K TPY + + +L+ +I+KVNG+ WDL++PL DS +E L+F+N+ A+ +W S+A +L +E + + GF D ++++ +SS + ++E+ + FER+++ + ++F ++ K +++ + ++YRC LID+C+ P VR+TG++K +KI ++ S +LQRIYG+SFP+ +++ W QEEA R+HR IG+EQELFFFH+ SPGS F LPRG I N L+D IRE+Y KR F+EV +P ++ ++LWETSGHW HY N+F ++ K+ F++KPMNCP HCL+++H+ R +++P+R ADFG LHR EL+G LSGLTR+R+F QDDAHIFC +QIE+EI+ L F++ VY F F L+LSTRP++FLG+IEIW+ AE QL+ SL +FGK W +N GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 119 FIKERLQLFETLKTDH-------QLLPATQEKKNTNNVISVRVAGGKTVQGERWKTTPYQVAAGISKELAEHTVIAKVNGVLWDLDRPLEGDSTVELLMFDNEEAQAVYWHSSAHILGEAMELYYGGHLCYGPPIENGFYYDMFIEDRVVSSTELSALENICKTIIKEKQPFERLEVSKDTLLEMFKYNKFKCRILKE-KVDTPTTTVYRCGPLIDLCKGPHVRHTGKIKAIKIFKNSSTYWEGNPEMETLQRIYGISFPDSKMMKDWEKFQEEAKSRDHRKIGKEQELFFFHDLSPGSCFFLPRGAFIYNALMDFIREEYHKRNFTEVLSPNMYNSKLWETSGHWQHYSNNMFTFDVE-KDTFALKPMNCPGHCLMFAHRPRSWREMPVRFADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCMVEQIEEEIKGCLHFLQSVYSTFGFSFQLNLSTRPEHFLGEIEIWDEAERQLQNSLVEFGKPWKINPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTY 642 HSP 2 Score: 107.071 bits (266), Expect = 1.976e-23 Identity = 56/125 (44.80%), Postives = 80/125 (64.00%), Query Frame = 2
Query: 1781 RPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDLRN-LRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
RPVIIHRAILGSVER +AIL E+ GKWP WL+PRQV+V+ V YA +V ++ + GF+ D DL + + +KI N + +YN IL++G+KE + V+VR + H + +I+K K
Sbjct: 652 RPVIIHRAILGSVERMIAILSENYGGKWPLWLSPRQVMVIPVGPACENYALQVSKECFEEGFMADVDLDDSCTLNKKIRNAQLAQYNFILVVGEKEKINNAVNVRTRDNKIHGEISIASVIEKLK 776
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Mouse
Match: Tars2 (threonyl-tRNA synthetase 2, mitochondrial (putative) [Source:MGI Symbol;Acc:MGI:1919057]) HSP 1 Score: 463.381 bits (1191), Expect = 2.499e-153 Identity = 229/531 (43.13%), Postives = 320/531 (60.26%), Query Frame = 3
Query: 174 LWKDLLDKNTKYSDDD-KFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFIL-KNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKS----------------KEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
LW + K + + IK+ G + TPY + + L+++ + ++VNG +DL++PL D + FL F++ + FW S+A +L E + R S + GF DF L K +++ S + +E +A+ F R++ + R LF + KL LIE ++Y C +D+C P +R+TGQ+ LK++ + S S +LQR+ G+SFP ELL W +E A R+HR IG+EQELFFFH SPGS F LPRGT + N L+ IR +Y +RGFSEV TP +F +LWE SGHW+HY+ ++F + K+ ++KPMNCPAHCL+++H+ R ++LP+RLADFGALHR E +G L GLTRL +F QDDAHIFC P Q+E EI+ LDF++ VY +L F F L LSTRP FLG+ +W+ AE+ L+++LEKFG+ W LN GDGAFYGPKID+ + DAL R HQC TIQLDFQLPLRF+L+Y
Sbjct: 47 LWTAHVKKLASMTQKKARAIKISLPEGQKVDAVAWNTTPYQLAHQISVTLADTAVAAEVNGELYDLDRPLETDCHLRFLTFDSPEGKAVFWHSSAHVLGAAAEQQLGAVLCRGPSTESGFYHDFFLGKERTVRSAELPILERICQELIAAAQPFRRLEASRDQLRQLFKDNHFKLHLIEE-KVTGPTATVYGCGMSVDLCRGPHLRHTGQIGALKLLTNSSALWRSLGAPETLQRVSGISFPKVELLRNWEARREAAELRDHRRIGKEQELFFFHELSPGSCFFLPRGTRVYNALVAFIRAEYARRGFSEVKTPTLFSTKLWEQSGHWEHYRADMFSLKPPGTDGVDNSQSGHPARCPKDTLALKPMNCPAHCLMFAHRPRSWRELPVRLADFGALHRAEASGSLGGLTRLWRFQQDDAHIFCAPHQLEAEIQGCLDFLRCVYSVLGFSFHLALSTRPPGFLGEPRLWDQAEQVLQQALEKFGEPWDLNPGDGAFYGPKIDVHLHDALGRPHQCGTIQLDFQLPLRFDLQY 576 HSP 2 Score: 100.138 bits (248), Expect = 2.907e-21 Identity = 52/128 (40.62%), Postives = 76/128 (59.38%), Query Frame = 2
Query: 1769 GSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
G+ E PV+IHRA+LGSVER L +L ES GKWP WL+P QV+V+ V + EYA +V Q ++ AG + D D L ++R++ + YN ++GQ+E R V+VR DL E +++
Sbjct: 581 GTPECPVLIHRAVLGSVERLLGVLAESCGGKWPLWLSPLQVVVIPVRTEQEEYARQVQQCLQAAGLVSDLDADSGLTLSRRVRRAQLAHYNFQFVVGQREQSQRTVNVRTRDNRQLGERDLAESVQRL 708
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Mouse
Match: Tars2 (threonyl-tRNA synthetase 2, mitochondrial (putative) [Source:MGI Symbol;Acc:MGI:1919057]) HSP 1 Score: 408.683 bits (1049), Expect = 8.168e-137 Identity = 211/531 (39.74%), Postives = 298/531 (56.12%), Query Frame = 3
Query: 174 LWKDLLDKNTKYSDDD-KFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFIL-KNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKS----------------KEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
LW + K + + IK+ G + TPY + + L+++ + ++VNG +DL++PL D + FL F++ + FW S+A +L E + R S + GF DF L K +++ S + +E +A+ F R++ + R LF + KL LIE ++Y C +D+C P +R+TGQ+ LK++ + S S +LQR+ G+SFP ELL W +E A R+HR IG+EQELFFFH SPGS F LPRGT + N L+ IR +Y +RGFSEV TP +F +LWE SGHW+HY+ ++F + K+ ++KPMNCPAHCL+++H+ R ++LP+RLADFGALHR E +G L GLTRL +F QDDAHIFC P Q+E EI+ LDF++ VY +L F F L LSTRP FLG+ +W+ AE+ ID+ + DAL R HQC TIQLDFQLPLRF+L+Y
Sbjct: 47 LWTAHVKKLASMTQKKARAIKISLPEGQKVDAVAWNTTPYQLAHQISVTLADTAVAAEVNGELYDLDRPLETDCHLRFLTFDSPEGKAVFWHSSAHVLGAAAEQQLGAVLCRGPSTESGFYHDFFLGKERTVRSAELPILERICQELIAAAQPFRRLEASRDQLRQLFKDNHFKLHLIEE-KVTGPTATVYGCGMSVDLCRGPHLRHTGQIGALKLLTNSSALWRSLGAPETLQRVSGISFPKVELLRNWEARREAAELRDHRRIGKEQELFFFHELSPGSCFFLPRGTRVYNALVAFIRAEYARRGFSEVKTPTLFSTKLWEQSGHWEHYRADMFSLKPPGTDGVDNSQSGHPARCPKDTLALKPMNCPAHCLMFAHRPRSWRELPVRLADFGALHRAEASGSLGGLTRLWRFQQDDAHIFCAPHQLEAEIQGCLDFLRCVYSVLGFSFHLALSTRPPGFLGEPRLWDQAEQ--------------------------IDVHLHDALGRPHQCGTIQLDFQLPLRFDLQY 550 HSP 2 Score: 100.138 bits (248), Expect = 8.168e-137 Identity = 52/128 (40.62%), Postives = 76/128 (59.38%), Query Frame = 2
Query: 1769 GSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
G+ E PV+IHRA+LGSVER L +L ES GKWP WL+P QV+V+ V + EYA +V Q ++ AG + D D L ++R++ + YN ++GQ+E R V+VR DL E +++
Sbjct: 555 GTPECPVLIHRAVLGSVERLLGVLAESCGGKWPLWLSPLQVVVIPVRTEQEEYARQVQQCLQAAGLVSDLDADSGLTLSRRVRRAQLAHYNFQFVVGQREQSQRTVNVRTRDNRQLGERDLAESVQRL 682
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Mouse
Match: Tars2 (threonyl-tRNA synthetase 2, mitochondrial (putative) [Source:MGI Symbol;Acc:MGI:1919057]) HSP 1 Score: 396.356 bits (1017), Expect = 4.333e-133 Identity = 186/358 (51.96%), Postives = 242/358 (67.60%), Query Frame = 3
Query: 687 KLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKS----------------KEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
KL LIE ++Y C +D+C P +R+TGQ+ LK++ + S S +LQR+ G+SFP ELL W +E A R+HR IG+EQELFFFH SPGS F LPRGT + N L+ IR +Y +RGFSEV TP +F +LWE SGHW+HY+ ++F + K+ ++KPMNCPAHCL+++H+ R ++LP+RLADFGALHR E +G L GLTRL +F QDDAHIFC P Q+E EI+ LDF++ VY +L F F L LSTRP FLG+ +W+ AE+ L+++LEKFG+ W LN GDGAFYGPKID+ + DAL R HQC TIQLDFQLPLRF+L+Y
Sbjct: 139 KLHLIEE-KVTGPTATVYGCGMSVDLCRGPHLRHTGQIGALKLLTNSSALWRSLGAPETLQRVSGISFPKVELLRNWEARREAAELRDHRRIGKEQELFFFHELSPGSCFFLPRGTRVYNALVAFIRAEYARRGFSEVKTPTLFSTKLWEQSGHWEHYRADMFSLKPPGTDGVDNSQSGHPARCPKDTLALKPMNCPAHCLMFAHRPRSWRELPVRLADFGALHRAEASGSLGGLTRLWRFQQDDAHIFCAPHQLEAEIQGCLDFLRCVYSVLGFSFHLALSTRPPGFLGEPRLWDQAEQVLQQALEKFGEPWDLNPGDGAFYGPKIDVHLHDALGRPHQCGTIQLDFQLPLRFDLQY 495 HSP 2 Score: 100.138 bits (248), Expect = 4.333e-133 Identity = 52/128 (40.62%), Postives = 76/128 (59.38%), Query Frame = 2
Query: 1769 GSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
G+ E PV+IHRA+LGSVER L +L ES GKWP WL+P QV+V+ V + EYA +V Q ++ AG + D D L ++R++ + YN ++GQ+E R V+VR DL E +++
Sbjct: 500 GTPECPVLIHRAVLGSVERLLGVLAESCGGKWPLWLSPLQVVVIPVRTEQEEYARQVQQCLQAAGLVSDLDADSGLTLSRRVRRAQLAHYNFQFVVGQREQSQRTVNVRTRDNRQLGERDLAESVQRL 627
BLAST of Threonine--tRNA ligase, cytoplasmic vs. UniProt/SwissProt
Match: sp|Q9D0R2|SYTC_MOUSE (Threonine--tRNA ligase 1, cytoplasmic OS=Mus musculus OX=10090 GN=Tars1 PE=1 SV=2) HSP 1 Score: 508.064 bits (1307), Expect = 9.231e-172 Identity = 245/504 (48.61%), Postives = 337/504 (66.87%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + D K IKV +G + K TPY I + L+++ +++KVN + WDL++PL D +E L FE++ A+ +W S+A ++ +E ++ + GF D L+ +SS F S+E + FER+++ E ++F ++ K ++ E++++ ++YRC LID+C P VR+TG++K LKI ++ S + +LQRIYG+SFP+ +LL W QEEA R+HR IG +QEL+FFH SPGS F LP+G +I N L++ IR +YRKRGF EV TP IF + LW TSGHW HY EN+F ++ KE+F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE EI+ LDF++ VY + F F L+LSTRP+ FLGDIEIWN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 74 KAAKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVVAKVNKVVWDLDRPLETDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLETLCKKIIKEKQTFERLEVKKETLLEMFKYNKFKCRILNEKVNTPT--TTVYRCGPLIDLCRGPHVRHTGKIKTLKIHKNSSTYWEGKADMETLQRIYGISFPDPKLLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNTLMEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVE-KEQFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEIWNQAEKQLENSLNEFGEKWELNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 574 HSP 2 Score: 119.783 bits (299), Expect = 9.231e-172 Identity = 60/126 (47.62%), Postives = 84/126 (66.67%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVI+HRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V EYA +V QQ DA F+ DTDL + +KI N + +YN IL++G+KE S V++R + H ++E +++ +
Sbjct: 583 KRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADTDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKASGTVNIRTRDNKVHGERTVEETVRRLQ 708
BLAST of Threonine--tRNA ligase, cytoplasmic vs. UniProt/SwissProt
Match: sp|Q5XHY5|SYTC_RAT (Threonine--tRNA ligase 1, cytoplasmic OS=Rattus norvegicus OX=10116 GN=Tars1 PE=1 SV=1) HSP 1 Score: 507.294 bits (1305), Expect = 4.733e-170 Identity = 245/504 (48.61%), Postives = 337/504 (66.87%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + D K IKV +G + K TPY I + L+++ +++KVN + WDL++PL D +E L FE++ A+ +W S+A ++ +E ++ + GF D L+ +SS F S+E + FER+++ E ++F ++ K ++ E++++ ++YRC LID+C P VR+TG++K LKI ++ S + +LQRIYG+SFP+ +LL W QEEA R+HR IG +QEL+FFH SPGS F LP+G +I N L++ IR +YRKRGF EV TP IF + LW TSGHW HY EN+F ++ KE+F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE EI+ LDF++ VY + F F L+LSTRP+ FLGDIEIWN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 47 KAAKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVVAKVNKVVWDLDRPLETDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLETLCKKIIKEKQTFERLEVKKETLLEMFKYNKFKCRILNEKVNTPT--TTVYRCGPLIDLCRGPHVRHTGKIKTLKIHKNSSTYWEGKADMETLQRIYGISFPDPKLLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNTLMEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVE-KEQFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEIWNQAEKQLENSLNEFGEKWELNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 547 HSP 2 Score: 114.775 bits (286), Expect = 4.733e-170 Identity = 60/115 (52.17%), Postives = 78/115 (67.83%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR----KGHGE 2107
+RPVI+HRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V EYA +V Q+ DA F+ D DL + +KI N + +YN IL++G+KE S V++R K HGE
Sbjct: 556 KRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQEFHDAKFMVDIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKASGTVNIRTRDNKVHGE 670
BLAST of Threonine--tRNA ligase, cytoplasmic vs. UniProt/SwissProt
Match: sp|P26639|SYTC_HUMAN (Threonine--tRNA ligase 1, cytoplasmic OS=Homo sapiens OX=9606 GN=TARS1 PE=1 SV=3) HSP 1 Score: 504.212 bits (1297), Expect = 1.663e-169 Identity = 245/504 (48.61%), Postives = 335/504 (66.47%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K D K IKV +G + K TPY I + L+++ +I+KVN + WDL++PL D +E L FE++ A+ +W S+A ++ +E ++ + GF D L+ +SS F S+E + FER+++ E +F ++ K ++ E++++ ++YRC LID+C P VR+TG++K LKI ++ S + +LQRIYG+SFP+ ++L W QEEA R+HR IG +QEL+FFH SPGS F LP+G +I N LI+ IR +YRKRGF EV TP IF + LW TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE EI+ LDF++ VY + F F L+LSTRP+ FLGDIE+W+ AE+QL+ SL +FG+ W LNSGDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 75 KAEKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLEALCKKIIKEKQAFERLEVKKETLLAMFKYNKFKCRILNEKVNTPT--TTVYRCGPLIDLCRGPHVRHTGKIKALKIHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVE-KELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTY 575 HSP 2 Score: 115.931 bits (289), Expect = 1.663e-169 Identity = 60/126 (47.62%), Postives = 82/126 (65.08%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVI+HRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V EYA +V QQ DA F+ D DL + +KI N + +YN IL++G+KE S V++R + H + E I++ +
Sbjct: 584 KRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQ 709
BLAST of Threonine--tRNA ligase, cytoplasmic vs. UniProt/SwissProt
Match: sp|Q54J66|SYTC1_DICDI (Probable threonine--tRNA ligase 1, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=thrS1 PE=3 SV=1) HSP 1 Score: 491.115 bits (1263), Expect = 6.247e-169 Identity = 237/516 (45.93%), Postives = 335/516 (64.92%), Query Frame = 3
Query: 174 LWKDLLDKNTKYSDD--DKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNG-LFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+W+ L K+ + D ++ I V +G + + K TPY I + + L++SI+ SKVNG WD+++PL D +++ F+++ + FW S+A +L +E ++ + + +GF D + +K +S+ ++ I + V FER+ +P +IA +F F+ K ++I ++ E+ +SLYRC L+D+C P V NT +K + ++ S + LQR+YG+SFP+K+ ++ + EA R+HR +G+ QELFFFH SPGSAF LP GT I NKL++ IRE+Y +RGF+EV +P IF +LWE SGHW Y EN+F + + K+ FS+KPMNCP HCL++ +QR ++LPLR ADFG LHR EL G L+GLTR+RKF QDDAHIFC + IE EI L F+++VYGI FEF L LSTRPDNFLG+I W++AE L+K+L KFGK W LN DGAFYGPKIDI I D L R HQCATIQLDFQLP+RF+L+Y
Sbjct: 56 IWESLKAKHLESLKDMKEEPINVTLPDGKVVAGIKNKTTPYDIAKGISRGLADSIVSSKVNGEQIWDISRPLEADCNLQLCKFDSEEGKKTFWHSSAHILGQAMERIYGGQLCIGPATSEGFYYDMAMGDKIVSAEDYKLINEVAQKIVQEKQPFERLAVPRDIALTMFKFNKYKTEIISKIPQEDT-VSLYRCGTLVDLCRGPHVPNTSYIKSFAVTKNSSAYWLGKAENDDLQRVYGISFPDKKQMEEYENFMREAALRDHRNVGKAQELFFFHPYSPGSAFFLPHGTRIYNKLVEFIREEYHRRGFTEVISPSIFSQKLWEQSGHWQKYSENMFVLPV-DKDNFSLKPMNCPGHCLMFGSRQRSYRELPLRFADFGVLHRNELAGALTGLTRVRKFQQDDAHIFCTTEMIEDEINSCLGFMQYVYGIFGFEFGLELSTRPDNFLGEIAQWDIAEASLEKALNKFGKPWKLNPKDGAFYGPKIDIHITDCLKRSHQCATIQLDFQLPIRFDLEY 569 HSP 2 Score: 127.102 bits (318), Expect = 6.247e-169 Identity = 59/125 (47.20%), Postives = 85/125 (68.00%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDLRNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AILIE GKWP W++PRQ +VV+V+ EY +VC++I DAGF CD D + +++K+ + +YN IL++GQ+E++ V+VR S + +LI +FK
Sbjct: 579 KRPVIIHRAILGSVERMMAILIEHTGGKWPLWVSPRQAIVVTVNKTHNEYGQKVCKEISDAGFYCDIDDSDKTISKKVREAQLAQYNYILVVGQEEINGNTVNVRTRDNVVRGSLTVNDLISEFK 703
BLAST of Threonine--tRNA ligase, cytoplasmic vs. UniProt/SwissProt
Match: sp|Q0V9S0|SYTC2_XENTR (Threonine--tRNA ligase 2, cytoplasmic OS=Xenopus tropicalis OX=8364 GN=tars3 PE=2 SV=1) HSP 1 Score: 501.901 bits (1291), Expect = 2.105e-168 Identity = 236/504 (46.83%), Postives = 335/504 (66.47%), Query Frame = 3
Query: 201 TKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+ + K IK+ +G + K TPY + + L+++++I+KVN WDL++PL DS++E L F+++ A+ +W S+A +L +E+ + + GF D L + +SS +F S+E+ + FER+++ ++ ++F ++ K ++ + ++YRC LID+C P VR+TG++K LKI ++ S + +LQRIYG+SFP+ +L+ W QEEA R+HR IG +QELFFFH+ SPGS F LPRG HI N L D I+ +Y+ R F+EV +P I+ ++LWE SGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLR ADFG LHR EL+G LSGLTR+R+F QDDAHIFC DQI++E+ L F++ VY + F F LHLSTRP+N+LG+IEIWN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 165 ARAAHQSKPIKITLADGKQVDGESWKTTPYQVAIGISKGLADNVVIAKVNNELWDLDRPLEQDSNVELLKFDSEEAQAVYWHSSAHILGETMENFYGGCLCYGPPIENGFYYDMYLDGRGVSSNEFPSLENMCKAIIKEKQPFERLEVSKDLLLEMFKYNKFKCRILNE-KVDTPTTTVYRCGPLIDLCRGPHVRHTGKIKTLKIYKNSSTYWEGRADMETLQRIYGISFPDSKLMKEWEQFQEEAKNRDHRKIGRDQELFFFHDLSPGSCFFLPRGAHIYNTLTDFIKGEYQIRNFTEVASPNIYNSKLWEMSGHWQHYSENMFSFEVE-KETFALKPMNCPGHCLMFGHRPRSWRELPLRFADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCTMDQIQEEMNGCLQFLQSVYKVFGFTFQLHLSTRPENYLGEIEIWNEAEKQLESSLNQFGEPWKLNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTY 666 HSP 2 Score: 114.775 bits (286), Expect = 2.105e-168 Identity = 59/126 (46.83%), Postives = 82/126 (65.08%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AIL E+ GKWPFWL+PRQV+V+ V EYA +VC+ + +AGF+ + DL + + +KI N + + N IL++G+KE V+VR + H L I+K K
Sbjct: 675 KRPVIIHRAILGSVERMIAILCENYGGKWPFWLSPRQVMVIPVGPSCDEYAQQVCKDVFEAGFMAEVDLDHSCTLNKKIRNAQLAQCNFILVVGEKEKTDSAVNVRTRDNKVHGEILLTSAIEKLK 800
BLAST of Threonine--tRNA ligase, cytoplasmic vs. TrEMBL
Match: A0A218UNN2 (Putative threonine--tRNA ligase 2, cytoplasmic OS=Lonchura striata domestica OX=299123 GN=TARSL2 PE=3 SV=1) HSP 1 Score: 525.013 bits (1351), Expect = 1.513e-175 Identity = 246/504 (48.81%), Postives = 348/504 (69.05%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+ ++ + IKV +G + K TPY + + L+++ +I+KVNG WDL++PL D +E L F+N+ A+ +W S+A +L +E F + GF D ++++S+SS +F +E++ N + FER+++ EI D+F ++ K ++ E++ + ++YRC LID+C+ P VR+TG++K LKI++S S + +LQRIYG+SFP+ +++ W QEEA R+HR IG+EQELFFFH+ SPGS F LPRG + N L+D IR +YR+R F+EV +P +F ++LWE SGHW HY EN+F ++ KE F++KPMNCP HCL+++H+ R ++LPLRLADFG LHR EL+G LSGLTR+R+F QDDAHIFC +QIE+EI+ LDF+K VY + F F LHLSTRP+N+LGD+EIW+ AE+QL+ SL FG+ W+LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 142 RAANQSRSIKVTLTDGETVEGESWKTTPYQLAVGISQVLASNAVIAKVNGELWDLDRPLEGDCTLELLTFDNEEAKAVYWHSSAHILGEAMEGYFGGCLCYGPPIENGFHYDMYIEDRSVSSTEFPLLENRCKNIIKEKQAFERLEVKKEILLDMFKYNKFKCRILNEKVKTPT--TTVYRCGPLIDLCKGPHVRHTGKIKALKIIKSSSTYWEGKSDMETLQRIYGISFPDNKMMKEWEKIQEEAKNRDHRKIGKEQELFFFHDLSPGSCFFLPRGAFLYNTLVDFIRGEYRRRNFTEVVSPNVFNSKLWEASGHWQHYSENMFSFEIE-KETFALKPMNCPGHCLMFAHRPRSWRELPLRLADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCTMEQIEEEIKDCLDFLKSVYAVFGFTFQLHLSTRPENYLGDLEIWDHAEKQLQNSLNNFGEQWNLNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTY 642 HSP 2 Score: 123.25 bits (308), Expect = 1.513e-175 Identity = 67/130 (51.54%), Postives = 90/130 (69.23%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR----KGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V +YA +VC Q +AGF+ D DL ++ + +KI N + +YN IL++G+KE + V+VR K HGE S +++L KKFK
Sbjct: 651 KRPVIIHRAILGSVERMIAILAENYGGKWPFWLSPRQVMVVPVGPTSEQYAQQVCDQFFEAGFMSDVDLDQSCTLNKKIRNAQLAQYNFILVVGEKEKANNAVNVRTRDNKVHGEISVSSTIEKL-KKFK 779
BLAST of Threonine--tRNA ligase, cytoplasmic vs. TrEMBL
Match: A0A091JGJ5 (Putative threonine--tRNA ligase 2, cytoplasmic (Fragment) OS=Egretta garzetta OX=188379 GN=Z169_06632 PE=3 SV=1) HSP 1 Score: 525.783 bits (1353), Expect = 1.646e-175 Identity = 244/504 (48.41%), Postives = 349/504 (69.25%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+ ++ K IK+ +G + + K TPY + + L+++ +I+KVNG WDL++PL D +E L+F+N+ A+ +W S+A +L +E F + GF D +++ +SS +F +E++ N + FER+++ EI D+F ++ K ++ E++++ ++YRC LID+C P VR+TG++K LKIV++ S + +LQRIYG+SFP+ +++ W QEEA R+HR IG+EQELFFFH+ SPGS F LPRG + N L+D IRE+Y +R F+EV +P IF ++LWE SGHW HY EN+F ++ KE F++KPMNCP HCL+++H+ R +++PLRLADFG LHR EL+G LSGLTR+R+F QDDAHIFC +QIE+EI+ LDF+K VY + F F LHLSTRP+N+LG++EIW+ AE+QL+ SL+ FG+ W+LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 25 RAANQSKTIKITLTDGKMAEGESWKTTPYQVAVGISQMLASNAVIAKVNGELWDLDRPLEGDCTLELLMFDNEEAKAVYWHSSAHILGEAMEGYFGGCLCYGPPIENGFYYDMYTEDRGVSSTEFPLLENRCKNIIKEKQAFERLEVKKEILLDMFKYNKFKCRILNEKVNTPT--TTIYRCGPLIDLCRGPHVRHTGKIKTLKIVKNSSTYWEGKSDMETLQRIYGISFPDNKMMKEWEKFQEEARNRDHRKIGKEQELFFFHDLSPGSCFFLPRGAFLYNTLVDFIREEYHRRNFTEVVSPNIFNSKLWEASGHWQHYSENMFSFEIE-KETFALKPMNCPGHCLMFAHRPRSWREMPLRLADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCTIEQIEEEIKGCLDFLKSVYAVFGFTFQLHLSTRPENYLGELEIWDHAEKQLQNSLDNFGEQWNLNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTY 525 HSP 2 Score: 122.479 bits (306), Expect = 1.646e-175 Identity = 67/130 (51.54%), Postives = 90/130 (69.23%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR----KGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V EYA +VC + +AGF+ D DL ++ + +KI N + +YN IL++G+KE + V+VR K HGE S +++L KKFK
Sbjct: 534 KRPVIIHRAILGSVERMIAILAENYGGKWPFWLSPRQVMVVPVGPASEEYAQQVCSKFFEAGFMSDVDLDQSCTLNKKIRNAQLAQYNFILVVGEKEKANNAVNVRTRDNKVHGEISVSSAIEKL-KKFK 662
BLAST of Threonine--tRNA ligase, cytoplasmic vs. TrEMBL
Match: A0A3L8SKS5 (AA_TRNA_LIGASE_II domain-containing protein OS=Chloebia gouldiae OX=44316 GN=DV515_00006282 PE=3 SV=1) HSP 1 Score: 524.628 bits (1350), Expect = 4.580e-175 Identity = 244/503 (48.51%), Postives = 345/503 (68.59%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+ ++ K IK+ +G + K TPY + + L+++ +I+KVNG WDL++PL D +E L F+N+ A+ +W S+A +L +E F + GF D ++++S+SS +F +E++ N + FER+++ EI D+F ++ K ++ + ++YRC LID+C+ P VR+TG++K LK+++S S + +LQRIYG+SFP+ +++ W QEEA R+HR IG+EQELFFFH+ SPGS F LPRG + N L+D IR +YR+R F+EV +P +F ++LWE SGHW HY EN+F ++ KE F++KPMNCP HCL+++H+ R ++LPLRLADFG LHR EL+G LSGLTR+R+F QDDAHIFC +QIE+EI+ LDF+K VY + F F LHLSTRP+N+LGD+EIW+ AE+QL+ SL FG+ W+LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 76 RAANQSKSIKITLTDGKTVEGESWKTTPYQLAVGISQVLASNAVIAKVNGELWDLDRPLEGDCTLELLTFDNEEAKAVYWHSSAHILGEAMEGYFGGCLCYGPPIENGFHYDMYMEDRSVSSTEFPLLENRCKNIIKEKQAFERLEVKKEILLDMFKYNKFKCRILNE-KVKTPTTTVYRCGPLIDLCKGPHVRHTGKIKALKVIKSSSTYWEGKSDMETLQRIYGISFPDNKMMKEWEKVQEEAKNRDHRKIGKEQELFFFHDLSPGSCFFLPRGAFLYNTLVDFIRGEYRRRNFTEVVSPNVFNSKLWEASGHWQHYSENMFSFEIE-KETFALKPMNCPGHCLMFAHRPRSWRELPLRLADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCTMEQIEEEIKDCLDFLKSVYAVFGFTFQLHLSTRPENYLGDLEIWDHAEKQLQNSLNNFGEQWNLNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTY 576 HSP 2 Score: 122.094 bits (305), Expect = 4.580e-175 Identity = 67/130 (51.54%), Postives = 90/130 (69.23%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR----KGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V +YA +VC Q +AGF+ D DL ++ + +KI N + +YN IL++G+KE + V+VR K HGE S +++L KKFK
Sbjct: 585 KRPVIIHRAILGSVERMIAILAENYGGKWPFWLSPRQVMVVPVGPASEQYAQQVCDQFFEAGFMSDVDLDQSCTLNKKIRNAQLAQYNFILVVGEKEKVNNAVNVRTRDNKIHGEISVSSTIEKL-KKFK 713
BLAST of Threonine--tRNA ligase, cytoplasmic vs. TrEMBL
Match: H0Z5V3 (Threonyl-tRNA synthetase like 2 OS=Taeniopygia guttata OX=59729 GN=TARSL2 PE=3 SV=1) HSP 1 Score: 523.857 bits (1348), Expect = 4.736e-175 Identity = 245/503 (48.71%), Postives = 345/503 (68.59%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+ ++ + IK+ +G + K TPY + + L+++ +I+KVNG WDL++PL D +E L F+N+ A+ +W S+A +L +E F + GF D ++++S+SS +F +E++ N + FER+++ EI D+F ++ K ++ + ++YRC LID+C+ P VR+TG++K LKI++S S + +LQRIYG+SFP+ +++ W QEEA R+HR IG+EQELFFFH+ SPGS F LPRG + N L+D IR +YR+R F+EV +P +F ++LWE SGHW HY EN+F ++ KE F+IKPMNCP HCL+++H+ R ++LPLRLADFG LHR EL+G LSGLTR+R+F QDDAHIFC +QIE+EI+ LDF+K VY + F F LHLSTRP+N+LGD+EIW+ AE+QL+ SL FG+ W+LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 25 RAANQSRSIKITWTDGETVEGESWKTTPYQLAVGISQVLASNAVIAKVNGELWDLDRPLEGDCTLELLTFDNEEAKAVYWHSSAHILGEAMEGYFGGCLCYGPPIENGFHYDMYIEDRSVSSTEFPLLENRCKNIIKEKQAFERLEVKKEILLDMFKYNKFKCRILNE-KVKTPTTTVYRCGPLIDLCKGPHVRHTGKIKALKIIKSSSTYWEGKSDMETLQRIYGISFPDNKMMKEWEKIQEEAKNRDHRKIGKEQELFFFHDLSPGSCFFLPRGAFLYNTLVDFIRGEYRRRNFTEVVSPNVFNSKLWEASGHWQHYSENMFSFEVE-KETFAIKPMNCPGHCLMFAHRPRSWRELPLRLADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCTMEQIEEEIKDCLDFLKSVYAVFGFTFQLHLSTRPENYLGDLEIWDHAEKQLQNSLNNFGEQWNLNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTY 525 HSP 2 Score: 122.865 bits (307), Expect = 4.736e-175 Identity = 67/130 (51.54%), Postives = 90/130 (69.23%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR----KGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V +YA +VC Q +AGF+ D DL ++ + +KI N + +YN IL++G+KE + V+VR K HGE S +++L KKFK
Sbjct: 534 KRPVIIHRAILGSVERMIAILAENYGGKWPFWLSPRQVMVVPVGPASEQYAQQVCDQFFEAGFMSDVDLDQSCTLNKKIRNAQLAQYNFILVVGEKEKANNAVNVRTRDNKVHGEISVSSTIEKL-KKFK 662
BLAST of Threonine--tRNA ligase, cytoplasmic vs. TrEMBL
Match: U3KDA9 (Threonyl-tRNA synthetase like 2 OS=Ficedula albicollis OX=59894 GN=TARSL2 PE=3 SV=1) HSP 1 Score: 522.702 bits (1345), Expect = 1.053e-174 Identity = 246/504 (48.81%), Postives = 346/504 (68.65%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+ ++ K IKV +G + + + TPY + + L+++ +I+KVNG WDL++PL D +E L F+N+ A+ +W S+A +L +E F + GF D ++++S+SS +F +E++ N + FER+++ EI D+F ++ K ++ E++ + ++YRC LID+C P VR+TG++K LKI++S S + +LQRIYG+SFP+ +++ W QEEA R+HR IG+EQELFFFH+ SPGS F LPRG + N L D IR +YR+R F+EV +P I+ ++LWE SGHW HY EN+F ++ KE F++KPMNCP HCL+++H+ R ++LPLRLADFG LHR EL+G LSGLTR+R+F QDDAHIFC +QIE+EI+ LDF+K VY + F F LHLSTRP+N+LGD EIW+ AE+QL+ SL FG+ W+LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 93 RAANQSKSIKVTLTDGEMVEGESWRTTPYELAVGISQVLASNAVIAKVNGELWDLDRPLEGDCTLELLTFDNEEAKAVYWHSSAHILGEAMEGYFGGCLCYGPPIENGFHYDMYIEDRSVSSTEFPLLENRCKNIIKEKQAFERLEVKKEILLDMFKYNKFKCRILNEKVKTPT--TTVYRCGPLIDLCRGPHVRHTGKIKALKIIKSSSTYWEGKSDMETLQRIYGISFPDNKMMKEWEKVQEEAKNRDHRKIGKEQELFFFHDLSPGSCFFLPRGAFLYNTLTDFIRGEYRRRNFTEVVSPNIYNSKLWEASGHWQHYSENMFSFEIE-KETFALKPMNCPGHCLMFAHRPRSWRELPLRLADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCTMEQIEEEIKGCLDFLKSVYAVFGFTFQLHLSTRPENYLGDSEIWDHAEKQLQNSLNNFGEQWNLNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTY 593 HSP 2 Score: 122.865 bits (307), Expect = 1.053e-174 Identity = 67/130 (51.54%), Postives = 90/130 (69.23%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR----KGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AIL E+ GKWPFWL+PRQV+VV V +YA +VC Q +AGF+ D DL ++ + +KI N + +YN IL++G+KE + V+VR K HGE S +++L KKFK
Sbjct: 602 KRPVIIHRAILGSVERMIAILAENYGGKWPFWLSPRQVMVVPVGPTSEQYAQQVCSQFFEAGFMSDVDLDQSCTLNKKIRNAQLAQYNFILVVGEKEKANNAVNVRTRDNKVHGEISVSSAIEKL-KKFK 730
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Cavefish
Match: tars3 (threonyl-tRNA synthetase 3 [Source:ZFIN;Acc:ZDB-GENE-081104-490]) HSP 1 Score: 491.5 bits (1264), Expect = 1.767e-167 Identity = 234/505 (46.34%), Postives = 334/505 (66.14%), Query Frame = 3
Query: 201 TKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFIL-KNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K + + + I V+ +G + TPY + + L+++ +I++V+G WDL++PL D +E L F+++ A+ +W S+A +L +E + + GF D L + K +SS +F +E + + FER++I E ++F ++ K ++ E ++YRC LID+C P VR+TG++K LKI ++ + + +LQRIYG+SFP+ ++L W QEEA R+HR IG++QELFFFH+ SPGS F LPRG +I N L + IRE+Y +RGF EV +P I+ ++LWETSGHW HY EN+F ++ ++ F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE E++ LDF++ VY + F F LHLSTRP+ FLGDI +WN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RF+L Y
Sbjct: 206 AKQAANSQPISVVLPDGQKVEAQSWITTPYQLACGISQGLADNCVIARVDGGLWDLDRPLQKDCSLELLRFDHEDAQAVYWHSSAHILGEAMERFYGGCLCYGPPIENGFYYDMFLDEQKGVSSTEFGDLETLCKSIMKDKQPFERLEISKETLLEMFKYNKFKCRILNE-KVETPTTTVYRCGPLIDLCRGPHVRHTGKIKALKIYKNSATYWEGRSDMETLQRIYGISFPDSKMLKEWERFQEEAKNRDHRKIGKDQELFFFHDLSPGSCFFLPRGAYIYNTLTEFIREEYWRRGFQEVASPNIYNSKLWETSGHWQHYSENMFSFPVE-QDVFALKPMNCPGHCLMFGHRPRSWRELPLRLADFGVLHRNELSGTLTGLTRVRRFQQDDAHIFCTMEQIESEMKGCLDFLRCVYDVFGFSFQLHLSTRPEKFLGDIAVWNQAEKQLENSLNEFGEPWKLNPGDGAFYGPKIDINIKDAIGRYHQCATIQLDFQLPIRFDLTY 708 HSP 2 Score: 119.013 bits (297), Expect = 1.767e-167 Identity = 60/114 (52.63%), Postives = 82/114 (71.93%), Query Frame = 2
Query: 1781 RPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR----KGHGE 2107
RPVIIHRAILGSVER +AIL E+ +GKWP WL+PRQV++V V+ EYA++VC+Q +AGF+ D DL + + +KI N + +YN IL++G+KE + V+VR K HGE
Sbjct: 718 RPVIIHRAILGSVERMIAILTENYAGKWPLWLSPRQVMLVPVNPSLEEYASKVCKQFVEAGFMADADLDSSCLLNKKIRNAQLAQYNFILVVGEKEKMTNSVNVRTRDNKVHGE 831
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Cavefish
Match: tars1 (threonyl-tRNA synthetase 1 [Source:NCBI gene;Acc:103026572]) HSP 1 Score: 497.664 bits (1280), Expect = 8.384e-167 Identity = 236/500 (47.20%), Postives = 337/500 (67.40%), Query Frame = 3
Query: 216 DDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
D K IK+ +G + + K TPY + + L+++ +I+KVN WDL++PL D ++ L F+++ A+ +W S+A ++ +E ++ + GF D L+N+ +SS F +E+ + FER++I E ++F ++ K ++ E++ + ++YRC LID+C P VR+TG++K LKI ++ S + SLQR+YG+SFP+ ++L W QEEA R+HR +G EQ+LFFFH+ SPGS F LP+G ++ N L++ IR +YRKRGF EV TP I+ ++LW+TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC DQIE EI+ LDF++ VY + F F L+LSTRP+ FLG+ E+W+ AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 81 DSKPIKITLPDGKVVDGESWKTTPYQVACGISQGLADNTVIAKVNNAVWDLDRPLEEDCSLQLLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMFLENEGVSSNDFPCLENLCKKIIKEKQPFERLEIKKETLLEMFKYNKFKCRILNEKVTTPT--TTVYRCGPLIDLCRGPHVRHTGKIKALKIHKNSSTYWEGKADMESLQRLYGISFPDPKMLKEWEKFQEEAKNRDHRKLGREQDLFFFHDLSPGSCFFLPKGAYLYNTLVEFIRSEYRKRGFQEVVTPNIYNSKLWQTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCTMDQIESEIKGCLDFLRTVYDVFGFTFKLNLSTRPEKFLGEPEVWDQAEKQLENSLNEFGEKWVLNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLSY 577 HSP 2 Score: 110.923 bits (276), Expect = 8.384e-167 Identity = 59/115 (51.30%), Postives = 75/115 (65.22%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR----KGHGE 2107
+RPVIIHRAILGSVER +AIL E+ GKWP WL+PRQV+VV V EYA V + +AG + D DL + +KI N + +YN IL++G+KE S V+VR K HGE
Sbjct: 586 KRPVIIHRAILGSVERMIAILTENYGGKWPLWLSPRQVMVVPVGPTCEEYAQRVQNEFHNAGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKSSDTVNVRTRDNKVHGE 700
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Cavefish
Match: ENSAMXT00000012886.2 (threonine--tRNA ligase, cytoplasmic-like [Source:NCBI gene;Acc:103023620]) HSP 1 Score: 461.84 bits (1187), Expect = 2.845e-154 Identity = 216/503 (42.94%), Postives = 316/503 (62.82%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K ++ + + V +G + TP I Q + ++ +VNG W+L +PL D ++ L F++ R WR+ A +L ++E F++ R +D CD+ L N S+L + +E++ R+++ + +LF +++L L E IS+YRC + +C PL+ +TG + K+++ V ++ + R+ GVSFP ++ + W+ QEEA +R+HR IG++QELFFF++ SPGS F LP+G HI N L D I+ +Y+ RGFSEV TP +F LWE SGHW+HY EN+F + + F++KPMNCPAHC++Y + R ++LPLR ADFGALHR E +G L GLTR+R+F QDDAHIFC P+Q+E+EI LDF++ VY + F F LSTRP ++LG+ +W+ AE+QL++SLE+FG+ W LN GDGAFYGPK+DI I+DA+ RQHQCATIQLDFQLP+RFNL+Y
Sbjct: 52 KAAEPGQNLSVRLADGKTVKAVAGITTPLQIAQRTRIK---GAVVGRVNGELWELARPLEADCELHLLSFDSLEGRQVVWRTGACVLGAVMETHFTAHVCREGVSDTALYCDYRLDN---SALSLEEVEERCKEMAGLKLPITRLELNVHELGELFQ-DALRLQLAEEQIQGKNTISVYRCGQSVGVCSGPLLPHTGFIHSFKMLQVSPVTLSGGTEALGVMRMIGVSFPGEKEKEQWVQEQEEARRRDHRRIGKDQELFFFNDVSPGSCFFLPKGAHIYNTLTDFIKSEYKSRGFSEVVTPTLFSTTLWERSGHWEHYSENMFTVKCEP-HTFALKPMNCPAHCVMYEQRVRSWRELPLRWADFGALHRNEHSGALGGLTRVRRFCQDDAHIFCTPEQLEEEITACLDFVRSVYCVFGFTFHCLLSTRPSSYLGETALWDSAEQQLERSLEQFGERWELNPGDGAFYGPKVDIQIRDAIGRQHQCATIQLDFQLPIRFNLQY 546 HSP 2 Score: 105.145 bits (261), Expect = 2.845e-154 Identity = 50/131 (38.17%), Postives = 81/131 (61.83%), Query Frame = 2
Query: 1763 NEGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDT-DLRNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
++G RPV++HRA+LGS+ER +AIL E+ GKWP WL+P QV+V+ V +Y EV ++K AGF+ D D + ++I + + +YN I ++G+KE +S VSVR G+ L++++ +
Sbjct: 549 SDGGMHRPVMVHRAVLGSLERMIAILAENFGGKWPLWLSPAQVMVLPVGGDSKQYGREVVHRLKKAGFMADVDDDHGATLNKRIRSAQLAQYNYIFVVGEKERESGTVSVRTRGGKQLGQRSLEDVMTSLE 679
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Cavefish
Match: mrpl39 (mitochondrial ribosomal protein L39 [Source:NCBI gene;Acc:103046218]) HSP 1 Score: 68.1662 bits (165), Expect = 5.653e-12 Identity = 48/187 (25.67%), Postives = 81/187 (43.32%), Query Frame = 3
Query: 282 TPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFEN---DIARDAFWRSTAFLLNGILEHLFSSE-------TYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFM----NYVASGADFERIDIPIEIARDLFSFSSMKLDLIER--LHSENKPISLYRCLDLIDICEAPLVRNTG 794
TPY ++ + +S ++ V+G W L++PL + L F++ + A+WRS A LL +LE F E F CDF+L + + + + F + +E +++ +A ++FS S K + +E S N +SLYRC + + PLV TG
Sbjct: 82 TPYSCSRHLTDWHVSSAALALVDGEPWHLHRPLTRSCSLTLLTFKDANPQLPNQAYWRSCAALLGQVLEGAFKEEYNVELLRIPEVPVTAGAFCCDFVLDPQLDAWIPTEESLRSFTRDANQLIHQNLPWEPLEVAPPVALEIFSHSRCKQEEVEEKAAQSPNGTVSLYRCGHHVVLAGGPLVGRTG 268
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Cavefish
Match: mrpl39 (mitochondrial ribosomal protein L39 [Source:NCBI gene;Acc:103046218]) HSP 1 Score: 67.781 bits (164), Expect = 1.093e-11 Identity = 48/187 (25.67%), Postives = 81/187 (43.32%), Query Frame = 3
Query: 282 TPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFEN---DIARDAFWRSTAFLLNGILEHLFSSE-------TYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFM----NYVASGADFERIDIPIEIARDLFSFSSMKLDLIER--LHSENKPISLYRCLDLIDICEAPLVRNTG 794
TPY ++ + +S ++ V+G W L++PL + L F++ + A+WRS A LL +LE F E F CDF+L + + + + F + +E +++ +A ++FS S K + +E S N +SLYRC + + PLV TG
Sbjct: 75 TPYSCSRHLTDWHVSSAALALVDGEPWHLHRPLTRSCSLTLLTFKDANPQLPNQAYWRSCAALLGQVLEGAFKEEYNVELLRIPEVPVTAGAFCCDFVLDPQLDAWIPTEESLRSFTRDANQLIHQNLPWEPLEVAPPVALEIFSHSRCKQEEVEEKAAQSPNGTVSLYRCGHHVVLAGGPLVGRTG 261
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Sea Lamprey
Match: tars1 (threonyl-tRNA synthetase 1 [Source:ZFIN;Acc:ZDB-GENE-041010-218]) HSP 1 Score: 494.197 bits (1271), Expect = 4.384e-166 Identity = 236/510 (46.27%), Postives = 345/510 (67.65%), Query Frame = 3
Query: 186 LLDKNTKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
LL++ T S K I V +G + K TPY + + L+++++I++VNG WDL++PL +D+ ++ L F+++ A+ +W S+A +L +E ++ + GF D L + +SS F S+E N + FER++I ++ ++F ++ K+ ++ E++++ ++YRC LID+C+ P VR+TG++K LKI ++ S + SLQR+YG+SFP+ ++L W QEEA R+HR IG++QELFFFH+ SPGS F LP+G I N L+D I+ +Y+KRGF+EV +P I+ ++LW+TSGHWD+Y EN+F ++ KE F++KPMNCP HCL++ H+ R ++LP+R ADFG LHR EL+G L+GLTR+R+F QDDAHIFC +QIE EI LDF++ VY + F F L LSTRP N++GD+E WN AE+QL+ SL KFG+ W LN GDGAFYGPKIDI IKD + R HQCATIQLDFQLP+RF+L +
Sbjct: 165 LLERATNQS---KPITVTLPDGKTVEAESWKTTPYQLAAGISKGLADNVVIARVNGNLWDLDRPLESDTTLQLLKFDDEEAQAVYWHSSAHILGEAMERVYGGCLCYGPPIENGFYYDMFLDDGGVSSNDFPSLEGLCKNIMKEKQPFERLEIQKDVLLEMFKYNKFKVRILNEKVNTPT--TTVYRCGPLIDLCQGPHVRHTGKIKALKIHKNSSTYWEGKADMESLQRVYGISFPDTKMLKEWERMQEEAKNRDHRKIGKDQELFFFHDLSPGSCFFLPKGAFIYNTLVDFIKSEYQKRGFTEVVSPNIYNSKLWKTSGHWDNYSENMFAFEVE-KEMFALKPMNCPGHCLMFDHRPRSWRELPIRFADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCTMEQIESEIAGCLDFLRCVYEVFGFNFKLFLSTRPKNYMGDVEEWNQAEKQLENSLNKFGEPWKLNPGDGAFYGPKIDIQIKDGIGRYHQCATIQLDFQLPIRFDLNF 668 HSP 2 Score: 109.383 bits (272), Expect = 4.384e-166 Identity = 58/115 (50.43%), Postives = 79/115 (68.70%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR----KGHGE 2107
+RPVIIHRAILGSVER +AIL E+ GKWP WL+P+QV+VV V ++A +V Q+ D GF+ +T+L L + +KI N + +YN IL++G+KE S V+VR K HGE
Sbjct: 677 KRPVIIHRAILGSVERMIAILTENYGGKWPLWLSPQQVMVVPVGPTCNDFAQKVWQRFHDVGFMVNTELDPGLTLNKKIRNAQLAQYNFILVVGEKEKLSDTVNVRTRDNKVHGE 791
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Sea Lamprey
Match: pars2 (prolyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-030131-4219]) HSP 1 Score: 53.1434 bits (126), Expect = 1.535e-7 Identity = 35/135 (25.93%), Postives = 58/135 (42.96%), Query Frame = 3
Query: 984 FFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYS-KDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIF 1385
++PG +LPR KL+ LI ++ +++ P + E W SG WD LF++ ++ + + P + A + + + S + LPLRL R E GL R R+F D + F
Sbjct: 21 LIQPAAPGCYHLLPRAVRAVEKLVSLIDQEMVSINGHKISMPCLTMAEAWRASGRWDLMGSELFRLRDRTGADLCLGPTHEEAVTRLLAAQGVASYRQLPLRLYQVTQKFRDE-PRPCHGLLRGREFLMKDLYTF 154
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Yeast
Match: THS1 (Threonyl-tRNA synthetase; essential cytoplasmic protein; human homolog TARS can complement yeast null mutant [Source:SGD;Acc:S000001340]) HSP 1 Score: 462.996 bits (1190), Expect = 1.063e-161 Identity = 220/509 (43.22%), Postives = 326/509 (64.05%), Query Frame = 3
Query: 228 IKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSD----IEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKN----------KSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+K++ +G + + + TP I + + L++ + ISKVNG WDL++P +++ +E L FE+D + FW S+A +L E + D GF + +++ +++S F +E N + FER+ + E +F +S K L++ + ++YRC LID+C P + +TG++K K++++ S +SLQR+YG+SFP+K+L+D L EA R+HR IG+EQELF F+ SPGS F LP GT I N L+DL+R +YRKRG+ EV TP ++ ++LWETSGHW +Y+EN+F ++ KE F +KPMNCP HCL++ ++R ++LP R+ADFG +HR E +G LSGLTR+R+F QDDAHIFC DQIE EIE +F++++YG+ FEF + LSTRP+ ++G IE W+ AE +L+ +L+K+G +W +N+GDGAFYGPKIDI+I DAL R HQCATIQLDFQLP RF L++
Sbjct: 75 LKIVLKDGAVKEATSWETTPMDIAKGISKSLADRLCISKVNGQLWDLDRPFEGEANEEIKLELLDFESDEGKKVFWHSSAHVLGESCECHLGAHICLGPPTDDGFFYEMAVRDSMKDISESPERTVSQADFPGLEGVAKNVIKQKQKFERLVMSKEDLLKMFHYSKYKTYLVQTKVPDGGATTVYRCGKLIDLCVGPHIPHTGRIKAFKLLKNSSCYFLGDATNDSLQRVYGISFPDKKLMDAHLKFLAEASMRDHRKIGKEQELFLFNEMSPGSCFWLPHGTRIYNTLVDLLRTEYRKRGYEEVITPNMYNSKLWETSGHWANYKENMFTFEVE-KETFGLKPMNCPGHCLMFKSRERSYRELPWRVADFGVIHRNEFSGALSGLTRVRRFQQDDAHIFCTHDQIESEIENIFNFLQYIYGVFGFEFKMELSTRPEKYVGKIETWDAAESKLESALKKWGGNWEINAGDGAFYGPKIDIMISDALRRWHQCATIQLDFQLPNRFELEF 582 HSP 2 Score: 125.561 bits (314), Expect = 1.063e-161 Identity = 57/107 (53.27%), Postives = 78/107 (72.90%), Query Frame = 2
Query: 1772 SFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDLRNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVR 2092
S+ERPV+IHRAILGSVER AIL E +GKWPFWL+PRQVLVV V K+ YA +V ++ DAGF D DL + +K+ NG+ +YN I I+G++E++ + V++R
Sbjct: 591 SYERPVMIHRAILGSVERMTAILTEHFAGKWPFWLSPRQVLVVPVGVKYQGYAEDVRNKLHDAGFYADVDLTGNTLQKKVRNGQMLKYNFIFIVGEQEMNEKSVNIR 697
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Yeast
Match: MST1 (Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(UAG)Q2 in vitro; lacks a typical editing domain, but has pre-transfer editing activity stimulated by the unusual tRNA-Thr [Source:SGD;Acc:S000001677]) HSP 1 Score: 288.5 bits (737), Expect = 2.376e-98 Identity = 141/265 (53.21%), Postives = 183/265 (69.06%), Query Frame = 3
Query: 957 LIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYR-KRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISL--KSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYG-ILNF---------EFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
++ + Q+LF SPGS F LP G I NKLI+ ++ Q + K GF+EV TP I+K LWE SGHW++Y +++FK+ + KEE+ +KPMNCP HCLI+ K R +LPLR +DF LHR E +G LSGLTRLRKFHQDD HIFC P Q++ EI SL I VY I F + ++ STRPD+F+GD+++WN AE+ LK+ LE+ GK W LN GDGAFYGPK+DI++ D L + HQ ATIQLDFQLP RF+LK+
Sbjct: 41 MVSQRQDLFMTDPLSPGSMFFLPNGAKIFNKLIEFMKLQQKFKFGFNEVVTPLIYKKTLWEKSGHWENYADDMFKVETTDEEKEEYGLKPMNCPGHCLIFGKKDRSYNELPLRFSDFSPLHRNEASGALSGLTRLRKFHQDDGHIFCTPSQVKSEIFNSLKLIDIVYNKIFPFVKGGSGAESNYFINFSTRPDHFIGDLKVWNHAEQVLKEILEESGKPWKLNPGDGAFYGPKLDIMVTDHLRKTHQVATIQLDFQLPERFDLKF 305 HSP 2 Score: 88.5817 bits (218), Expect = 2.376e-98 Identity = 42/128 (32.81%), Postives = 77/128 (60.16%), Query Frame = 2
Query: 1766 EGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIK------------------DAGFLCDTDLRNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRK 2095
+ S++RP++IHRA GS+ERF+A+LI+SN G+WPFWLNP Q +++ V+ K ++ ++C ++ D F D D+RN + +I + + Y+ ++I+G +EV ++ ++R+
Sbjct: 309 DNSYKRPIMIHRATFGSIERFMALLIDSNEGRWPFWLNPYQAVIIPVNTKNVQQL-DMCTALQKKLRNELEADDMEPVPLNDWHFNVDLDIRNEPVGYRIKSAILKNYSYLIIVGDEEVQLQKYNIRE 435
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Nematostella
Match: EDO31193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYN6]) HSP 1 Score: 441.81 bits (1135), Expect = 2.401e-152 Identity = 206/398 (51.76%), Postives = 276/398 (69.35%), Query Frame = 3
Query: 519 DKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
++G+ D L+ + +SS F S+++ FER+++ E ++F ++ K ++ + ++YRC LID+C P VR+TG++K + + ++ + SLQRIYG+SFPN + + W QEEA KR+HR IG +QELFFFH SPGS F LP+G I N LI+ I+EQY KRGF EV +P I+ +LWETSGHW HY E++F ++ KE +++KPMNCP HCL++ H+ R ++LPLR+ADFG LHR EL+G LSGLTR+R+F QDDAHIFC DQI QEI LDF+KHVYG+ F F L+LSTRP+ +LG IE+WN AE++L+ +L FG++W LN GDGAFYGPKIDI I+DAL R HQCATIQLDFQLP RFNL Y
Sbjct: 16 EEGYYYDMHLEGRQVSSNDFSSLDNLTKKITKEKQPFERLEMKKEDLLEMFKYNEFKCRILNN-KVKTPTTTVYRCGPLIDLCRGPHVRHTGKIKSIAVTKNSATYWEGNCEAESLQRIYGISFPNDKQMKEWKKFQEEAAKRDHRKIGRDQELFFFHELSPGSCFFLPKGAVIYNTLINFIKEQYWKRGFQEVVSPNIYSTKLWETSGHWQHYSEHMFSFEVE-KESYALKPMNCPGHCLMFDHRPRSWRELPLRMADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCTADQIGQEIISCLDFLKHVYGVFGFTFKLNLSTRPEKYLGKIELWNQAEKELEAALNGFGQAWVLNPGDGAFYGPKIDIQIQDALRRYHQCATIQLDFQLPERFNLTY 411 HSP 2 Score: 116.701 bits (291), Expect = 2.401e-152 Identity = 55/125 (44.00%), Postives = 81/125 (64.80%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
+RPVI+HRAILGSVER +AIL ES GKWPFWL+PRQ +V+ + K+ EYA +V I AG+ D DL + +K+ N + +YN I ++G+KE + V+VR + H + ++ ++ KF
Sbjct: 420 KRPVIVHRAILGSVERMIAILTESFGGKWPFWLSPRQAIVIPIGPKYDEYAQKVMSDILAAGYHVDADLDHGTTLNKKVRNAQLAQYNFIFVVGEKEATTGTVNVRTRDNKVHGEFSVEAVLNKF 544
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Nematostella
Match: EDO27279 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T9P4]) HSP 1 Score: 150.984 bits (380), Expect = 1.501e-55 Identity = 70/105 (66.67%), Postives = 83/105 (79.05%), Query Frame = 3
Query: 1398 QIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+I QEI LDF+KHVYG+ F F L+LSTRP+ +LG IE+WN AE++L+ +L FG+ W LN GDGAFYGPKIDI I+DAL R HQCATIQLDFQLP RFNL Y
Sbjct: 3 KIGQEIISCLDFLKHVYGVFGFTFKLNLSTRPEKYLGKIELWNQAEKELEAALNGFGQPWVLNPGDGAFYGPKIDIQIQDALRRYHQCATIQLDFQLPERFNLTY 107 HSP 2 Score: 84.7297 bits (208), Expect = 1.501e-55 Identity = 36/55 (65.45%), Postives = 45/55 (81.82%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVC 1942
+RPVI+HRAILGSVER +AIL ES GKWPFWL+PRQ +V+ + K+ EYA +VC
Sbjct: 116 KRPVIVHRAILGSVERMIAILTESFGGKWPFWLSPRQAIVIPIGPKYDEYAQKVC 170
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Medaka
Match: tars3 (threonine--tRNA ligase, cytoplasmic [Source:NCBI gene;Acc:101167193]) HSP 1 Score: 498.049 bits (1281), Expect = 5.914e-168 Identity = 239/505 (47.33%), Postives = 335/505 (66.34%), Query Frame = 3
Query: 201 TKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKN-KSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K S + K I V +G + TPY + + L+++ +IS+VNG WDL++PL D +E L F+N+ A+ +W S+A +L +E + + GF D L + K+ISS +F +E + + FER+++ E +F ++ K ++ S ++YRC LID+C P VR+TG++K +KI ++ S + +LQRIYG+SFP+ ++L W QEEA R+HR IG++QELFFFH+ SPGS F LPRG +I N L + IR++Y +RGF EV +P ++ ++LWETSGHW HY EN+F ++ ++ F++KPMNCP HCL++SH+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC DQIE E++ LDF++ VY + F F LHLSTRP+ +LGDIE+WN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL +
Sbjct: 127 AKRSQNSKDITVELPDGQKVAAKSWVTTPYQVACEISQGLADNAVISRVNGELWDLDRPLQQDCSLELLRFDNEDAQAVYWHSSAHVLGEAMERFYGGCLCYGPPIENGFYYDMFLGDQKAISSSEFGDLEVLCKSVMKEKQPFERLELSKETLLKMFKYNKFKCRILNEKVS-TPTTTVYRCGPLIDLCRGPHVRHTGKIKAMKIYKNSSTYWEGRSDMETLQRIYGISFPDSKMLKEWERFQEEAKTRDHRKIGKDQELFFFHDLSPGSCFFLPRGAYIYNTLTEFIRDEYWRRGFQEVASPNVYNSKLWETSGHWQHYSENMFSFPVE-EDVFALKPMNCPGHCLMFSHRPRSWRELPLRLADFGVLHRNELSGTLTGLTRVRRFQQDDAHIFCTMDQIESEMKGCLDFLRGVYNVFGFSFQLHLSTRPEKYLGDIEVWNQAEKQLENSLNEFGEPWKLNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTF 629 HSP 2 Score: 114.005 bits (284), Expect = 5.914e-168 Identity = 56/124 (45.16%), Postives = 84/124 (67.74%), Query Frame = 2
Query: 1781 RPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
RPVIIHRAILGSVER +AIL E+ +GKWP WL+P QV++V V+ EYA +VC+Q +AGF+ + DL + + +KI N + +YN IL++G+KE + +V+VR + H + E++ +
Sbjct: 639 RPVIIHRAILGSVERMIAILTENYAGKWPLWLSPCQVMLVPVNPSCEEYAKKVCKQFTEAGFMAEADLDSSCLLNKKIRNAQLAQYNFILVVGEKEKMTNRVNVRTRDNKVHGELSVSEVMARL 762
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Medaka
Match: tars3 (threonine--tRNA ligase, cytoplasmic [Source:NCBI gene;Acc:101167193]) HSP 1 Score: 497.664 bits (1280), Expect = 9.039e-168 Identity = 239/505 (47.33%), Postives = 335/505 (66.34%), Query Frame = 3
Query: 201 TKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKN-KSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
K S + K I V +G + TPY + + L+++ +IS+VNG WDL++PL D +E L F+N+ A+ +W S+A +L +E + + GF D L + K+ISS +F +E + + FER+++ E +F ++ K ++ S ++YRC LID+C P VR+TG++K +KI ++ S + +LQRIYG+SFP+ ++L W QEEA R+HR IG++QELFFFH+ SPGS F LPRG +I N L + IR++Y +RGF EV +P ++ ++LWETSGHW HY EN+F ++ ++ F++KPMNCP HCL++SH+ R ++LPLRLADFG LHR EL+G L+GLTR+R+F QDDAHIFC DQIE E++ LDF++ VY + F F LHLSTRP+ +LGDIE+WN AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL +
Sbjct: 162 AKRSQNSKDITVELPDGQKVAAKSWVTTPYQVACEISQGLADNAVISRVNGELWDLDRPLQQDCSLELLRFDNEDAQAVYWHSSAHVLGEAMERFYGGCLCYGPPIENGFYYDMFLGDQKAISSSEFGDLEVLCKSVMKEKQPFERLELSKETLLKMFKYNKFKCRILNEKVS-TPTTTVYRCGPLIDLCRGPHVRHTGKIKAMKIYKNSSTYWEGRSDMETLQRIYGISFPDSKMLKEWERFQEEAKTRDHRKIGKDQELFFFHDLSPGSCFFLPRGAYIYNTLTEFIRDEYWRRGFQEVASPNVYNSKLWETSGHWQHYSENMFSFPVE-EDVFALKPMNCPGHCLMFSHRPRSWRELPLRLADFGVLHRNELSGTLTGLTRVRRFQQDDAHIFCTMDQIESEMKGCLDFLRGVYNVFGFSFQLHLSTRPEKYLGDIEVWNQAEKQLENSLNEFGEPWKLNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTF 664 HSP 2 Score: 114.005 bits (284), Expect = 9.039e-168 Identity = 56/124 (45.16%), Postives = 84/124 (67.74%), Query Frame = 2
Query: 1781 RPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKF 2149
RPVIIHRAILGSVER +AIL E+ +GKWP WL+P QV++V V+ EYA +VC+Q +AGF+ + DL + + +KI N + +YN IL++G+KE + +V+VR + H + E++ +
Sbjct: 674 RPVIIHRAILGSVERMIAILTENYAGKWPLWLSPCQVMLVPVNPSCEEYAKKVCKQFTEAGFMAEADLDSSCLLNKKIRNAQLAQYNFILVVGEKEKMTNRVNVRTRDNKVHGELSVSEVMARL 797
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Medaka
Match: tars1 (threonine--tRNA ligase, cytoplasmic [Source:NCBI gene;Acc:101171337]) HSP 1 Score: 484.952 bits (1247), Expect = 9.120e-165 Identity = 235/505 (46.53%), Postives = 335/505 (66.34%), Query Frame = 3
Query: 204 KYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFIL-KNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+ + + + IKV +G + + K TPY + + L+++ +I+KVN WDL++PL +D ++ L F+++ A+ +W S+A +L +E ++ + GF D L N+ +SS F +E+ + FER++I E ++F ++ K ++ E++ + ++YRC LID+C P VR+TG++K LKI ++ S + +LQRIYG+SFP+ ++L W QEEA R+HR +G EQ+LFFFH SPGS F LP+G I N LI+ IR +YR RGF EV TP I+ ++LW TSGHW HY EN+F ++ KE F++KPMNCP HCL++ H+ R ++LPLR+ADFG LHR EL+G L+GLTR+R+F QDDAHIFC DQIE+EI+ L F++ VY + F F L+LSTRP+ FLG+ E+W+ AE+QL+ SL +FG+ W LN GDGAFYGPKIDI IKDA+ R HQCATIQLDFQLP+RFNL Y
Sbjct: 76 RAAKESRPIKVTLPDGKVLEAESWKTTPYQLACGISQGLADNTVIAKVNNSVWDLDRPLEDDCSLQLLKFDDEEAQAVYWHSSAHILGEAMEKVYGGCLCYGPPIESGFYYDMFLDNNEGVSSNDFPCLENLCKKIIKEKQPFERLEIKKETLLEMFKYNKFKCRILNEKVTTPT--TTVYRCGPLIDLCRGPHVRHTGKIKALKIHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKLGREQDLFFFHELSPGSCFFLPKGAFIYNTLIEFIRSEYRNRGFQEVVTPNIYNSKLWVTSGHWQHYSENMFSFEVE-KEIFALKPMNCPGHCLMFDHRPRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCSMDQIEEEIKGCLHFLRAVYDVFGFTFKLNLSTRPEKFLGEPEMWDQAEKQLENSLNEFGEKWVLNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLAY 577 HSP 2 Score: 116.701 bits (291), Expect = 9.120e-165 Identity = 59/126 (46.83%), Postives = 83/126 (65.87%), Query Frame = 2
Query: 1778 ERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFK 2152
+RPVIIHRAILGSVER +AIL E+ GKWP WL+PRQV++V V EYA +V Q+ +GF+ D DL + + +KI N + +YN IL++G+KE S V+VR + H ++E I++ K
Sbjct: 586 KRPVIIHRAILGSVERMIAILTENYGGKWPLWLSPRQVMLVPVGPTCEEYAEKVKQEFHSSGFMADVDLDPSCTLNKKIRNAQLSQYNFILVVGEKEKTSNTVNVRTRDNKVHGECSVEECIQRLK 711
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Medaka
Match: ENSORLT00000040837.1 (threonine--tRNA ligase, cytoplasmic [Source:NCBI gene;Acc:101157805]) HSP 1 Score: 464.151 bits (1193), Expect = 1.994e-154 Identity = 233/498 (46.79%), Postives = 325/498 (65.26%), Query Frame = 3
Query: 219 DKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+K +++ NG TP + Q+ + L+SKVNG W+L +PL D +++ L F+ + A WR+ A +L G+LE F++E YR + GF CD +L N S+L +E + A R+D+ IE R LF S ++L L E + +++YRC D + +C +PL+ +TG LK K+++ SV + + L+R+ GV+FP ++ + W QEEA +R+HR IG +QELFFFH+ SPGS F LP+G HI N L D I+ +YR+RGFSEV TP ++ LWE SGHW+HY EN+F ++L+ + F++KPMNCPAHCL++ + R ++LPLR ADFGALHR EL+G L GLTR+R+F QDDAHIFC +Q+E EI LDF++ VY + F F LSTRP LG+ E W+ AE+QL+ SL++FG+ W LN GDGAFYGPK+DI+IKDA+ RQHQCATIQLDFQLP RF+L+Y
Sbjct: 53 EKPLRIHLGNGRTVKGTASVTTPLTVAQSVRVK---GALVSKVNGELWELGRPLEADCELQLLGFDTVEGKQAAWRTAACILGGVLERTFAAEVYREGATAFGFFCDHVLDN---SALSLTDVELQCQAAAALNLPLSRLDLNIEEFRKLFQGSDLRLQLAED-QTNGSTVTVYRCGDSLAVCSSPLLPHTGFLKVFKMLQLSSVTLGDQTGPSDLRRLLGVAFPGEKEREEWEREQEEARRRDHRRIGTDQELFFFHDVSPGSCFFLPKGAHIYNTLTDFIKSEYRRRGFSEVVTPTLYSTALWERSGHWEHYSENMFTVTLEDSQTFALKPMNCPAHCLMFEQRVRSWRELPLRWADFGALHRNELSGALGGLTRVRRFCQDDAHIFCTTEQLEVEILSCLDFVRSVYQVFGFSFHCLLSTRPTPCLGEPEQWDNAEQQLQSSLQQFGEHWELNPGDGAFYGPKVDILIKDAIGRQHQCATIQLDFQLPSRFDLQY 543 HSP 2 Score: 86.6557 bits (213), Expect = 3.198e-17 Identity = 40/95 (42.11%), Postives = 59/95 (62.11%), Query Frame = 2
Query: 1766 EGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCIL 2047
+G RPV+IHRA+LGS+ER +AIL E+ GKWP WL+P Q++V+ YA +V QQ +AGF+ D + + +KI + + +YN I
Sbjct: 547 DGQLHRPVMIHRAVLGSLERMMAILAENFGGKWPLWLSPAQIMVLPAGGNSEPYARQVVQQFNEAGFMVDLNADQGTTFNKKIRSAQLAQYNYIF 641
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Medaka
Match: ENSORLT00000013023.2 (threonine--tRNA ligase, cytoplasmic [Source:NCBI gene;Acc:101157805]) HSP 1 Score: 464.151 bits (1193), Expect = 4.084e-154 Identity = 233/498 (46.79%), Postives = 325/498 (65.26%), Query Frame = 3
Query: 219 DKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKY 1712
+K +++ NG TP + Q+ + L+SKVNG W+L +PL D +++ L F+ + A WR+ A +L G+LE F++E YR + GF CD +L N S+L +E + A R+D+ IE R LF S ++L L E + +++YRC D + +C +PL+ +TG LK K+++ SV + + L+R+ GV+FP ++ + W QEEA +R+HR IG +QELFFFH+ SPGS F LP+G HI N L D I+ +YR+RGFSEV TP ++ LWE SGHW+HY EN+F ++L+ + F++KPMNCPAHCL++ + R ++LPLR ADFGALHR EL+G L GLTR+R+F QDDAHIFC +Q+E EI LDF++ VY + F F LSTRP LG+ E W+ AE+QL+ SL++FG+ W LN GDGAFYGPK+DI+IKDA+ RQHQCATIQLDFQLP RF+L+Y
Sbjct: 63 EKPLRIHLGNGRTVKGTASVTTPLTVAQSVRVK---GALVSKVNGELWELGRPLEADCELQLLGFDTVEGKQAAWRTAACILGGVLERTFAAEVYREGATAFGFFCDHVLDN---SALSLTDVELQCQAAAALNLPLSRLDLNIEEFRKLFQGSDLRLQLAED-QTNGSTVTVYRCGDSLAVCSSPLLPHTGFLKVFKMLQLSSVTLGDQTGPSDLRRLLGVAFPGEKEREEWEREQEEARRRDHRRIGTDQELFFFHDVSPGSCFFLPKGAHIYNTLTDFIKSEYRRRGFSEVVTPTLYSTALWERSGHWEHYSENMFTVTLEDSQTFALKPMNCPAHCLMFEQRVRSWRELPLRWADFGALHRNELSGALGGLTRVRRFCQDDAHIFCTTEQLEVEILSCLDFVRSVYQVFGFSFHCLLSTRPTPCLGEPEQWDNAEQQLQSSLQQFGEHWELNPGDGAFYGPKVDILIKDAIGRQHQCATIQLDFQLPSRFDLQY 553 HSP 2 Score: 100.523 bits (249), Expect = 1.553e-21 Identity = 47/115 (40.87%), Postives = 72/115 (62.61%), Query Frame = 2
Query: 1766 EGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDL-RNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGE 2107
+G RPV+IHRA+LGS+ER +AIL E+ GKWP WL+P Q++V+ YA +V QQ +AGF+ D + + +KI + + +YN I ++G KE +S V++R G+
Sbjct: 557 DGQLHRPVMIHRAVLGSLERMMAILAENFGGKWPLWLSPAQIMVLPAGGNSEPYARQVVQQFNEAGFMVDLNADQGTTFNKKIRSAQLAQYNYIFVVGDKENESGTVNIRSRGGK 671
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Planmine SMEST
Match: SMESG000047630.1 (SMESG000047630.1) HSP 1 Score: 1127.46 bits (2915), Expect = 0.000e+0 Identity = 550/554 (99.28%), Postives = 552/554 (99.64%), Query Frame = 3
Query: 57 MILRKIHYFSFVNKLILNKFLKRSLSENELLKVQQIKQFLWKDLLDKNTKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKYFP 1718
MILRKIHYFSFVNKLILNKFLKR+LSE ELLKVQQIKQFLWKDLLDKNTKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIE LHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNR+HQCATIQLDFQLPLRFNLKYFP
Sbjct: 1 MILRKIHYFSFVNKLILNKFLKRNLSEKELLKVQQIKQFLWKDLLDKNTKYSDDDKFIKVMTLNGHLNYKLNEKPTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIEMLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRRHQCATIQLDFQLPLRFNLKYFP 554 HSP 2 Score: 275.404 bits (703), Expect = 4.510e-82 Identity = 131/133 (98.50%), Postives = 133/133 (100.00%), Query Frame = 2
Query: 1763 NEGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDLRNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFKYWE 2161
NEGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANE+CQQIKDAGFLCDTDLRNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDL+ELIKKFKYWE
Sbjct: 555 NEGSFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEICQQIKDAGFLCDTDLRNLRMARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLEELIKKFKYWE 687
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Planmine SMEST
Match: SMESG000061707.1 (SMESG000061707.1) HSP 1 Score: 497.664 bits (1280), Expect = 1.806e-167 Identity = 228/479 (47.60%), Postives = 331/479 (69.10%), Query Frame = 3
Query: 282 TPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFENDIARDAFWRSTAFLLNGILEHLFSSETYRSHSNDKGFICDFILKNKSISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLI-ERLHSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFPNKELLDGWLLHQEEAGKRNHRLIGEEQELFFFHNSSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMNCPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIFCRPDQIEQEIERSLDFIKHVYGILNFEFDLHLSTRPDNFLGDIEIWNLAEEQLKKSLEKFGKSWSLNSGDGAFYGPKIDIIIKDALNRQHQCATIQLDFQLPLRFNLKYF 1715
+PYI M+ ++++ +I+KVNG+ WDL++PL NDS +E L F++ FW S+A +L +E ++ + ++GF D LK++ + +++++++K V FER+++ I+ ++F ++ K ++ ER+ ++ ++YRC DLID+C+ P VRNTG++K L ++++ + SLQRIYG+SFP+ + + W QEEA KR+HR IG +Q+LF FH SPGS F LP+GT I NKL+D ++EQY RGF+EV +P ++ ++LWETSGHW+HY+EN+F+ ++ K +F++KPMNCP HCL++ R +DLP+RLADFG LHR E +G L+GLTR+R+F QDDAHIFCR DQI +EIE LDF+ + Y + F+F L LSTRP+ FLG+I +W+ AE QL +L FGK W LN DGAFYGPKIDI I DAL R HQCATIQLDFQLP+RF+L+Y
Sbjct: 52 SPYICMKEICQSVADNAVIAKVNGVLWDLDRPLENDSSLELLNFDSKDGEYVFWHSSAHILGEAMELEYAGHLCYGPAIEEGFYYDMFLKDRQVKPEEYKTLDEKCKKIVKEKQKFERLEVSIDRLLEMFDYNEFKCRILKERVKTDK--TTIYRCGDLIDLCQGPHVRNTGKVKALSVIKNSASYWEGDATRESLQRIYGISFPDPKQMKEWKTIQEEAAKRDHRKIGTKQQLFMFHELSPGSCFFLPKGTVIYNKLVDFMKEQYWTRGFTEVISPNLYNSKLWETSGHWEHYKENMFQTEIE-KCKFAMKPMNCPGHCLMFGSTIRSYRDLPIRLADFGVLHRNEASGALTGLTRVRRFQQDDAHIFCREDQITKEIESCLDFLDYTYRVFGFDFQLKLSTRPEKFLGEISMWDKAEAQLTDALNNFGKKWELNPADGAFYGPKIDITITDALRRHHQCATIQLDFQLPIRFSLEYM 527 HSP 2 Score: 109.768 bits (273), Expect = 1.178e-24 Identity = 54/131 (41.22%), Postives = 85/131 (64.89%), Query Frame = 2
Query: 1772 SFERPVIIHRAILGSVERFLAILIESNSGKWPFWLNPRQVLVVSVSDKFIEYANEVCQQIKDAGFLCDTDLRNLRM-ARKILNGEQQRYNCILIIGQKEVDSRQVSVRKGHGEDHESWDLQELIKKFKYWE 2161
+ RPVIIHRAILGSVER AIL E+ GKWPFWL+P Q +V+ + EYA + + + DAGF D DL + +KI ++ +YN +L++G+KEV+++ V++R + H ++++I++F+ E
Sbjct: 541 TLARPVIIHRAILGSVERMFAILAENYGGKWPFWLSPFQAIVIPFNPSCDEYALSIQKAVHDAGFHVDIDLDTKSIFNKKISKAQEAQYNFMLVVGKKEVENKTVNIRTRDNKVHGERSIEKVIERFRELE 671
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Planmine SMEST
Match: SMESG000067020.1 (SMESG000067020.1) HSP 1 Score: 65.0846 bits (157), Expect = 5.187e-11 Identity = 66/267 (24.72%), Postives = 120/267 (44.94%), Query Frame = 3
Query: 180 KDLLDK--------NTKYSDDDKFIKVMTLNGHLNYKLNEK-PTPYIIMQNDNNQLSNSILISKVNGLFWDLNKPLVNDSDIEFLLFEND---IARDAFWRSTAFLLNGILEHLFSSE------TYRSHSNDKG-FICDF-ILKNKS-------ISSLQFQSIEDKFMNYVASGADFERIDIPIEIARDLFSFSSMKLDLIERL--HSENKPISLYRCLDLIDICEAPLVRNTGQLKYLKIVRSGSVQMNSTDNCNSLQRIYGVSFP 893
+DL DK NT++ + + L+ + K+N+ TP+ + L+N +++ VN D++KPL D ++EFL F +D +FWRS +F+ N ILE+ F + ++ + G F+CD IL S I+++ +SI + ++ + + A ++F +K+ I + ++ I +Y+ +DIC PL+ NT + + SV T++ L R GV+ P
Sbjct: 22 RDLFDKEFNENLQLNTRHLEKVT-VHCKNLSQQITLKMNKNISTPHDCAIHIQEYLANKSVMALVNNQPHDMHKPLFEDCELEFLNFHSDNCIPVNLSFWRSCSFITNWILENCFHEDFQVNLCSFPYPNLQSGSFVCDVQILYKDSPEFDEQLITTVNLKSISTTARKIMLENYNYIPLSVNQSTAEEMFRDCPLKMKQIPFMMENANQNKIRIYKVGQHVDICRGPLISNTSHVGRYSLT---SVYPIKTNDYGRLFRFQGVAIP 284
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Planmine SMEST
Match: SMESG000039017.1 (SMESG000039017.1) HSP 1 Score: 51.9878 bits (123), Expect = 1.175e-6 Identity = 42/179 (23.46%), Postives = 84/179 (46.93%), Query Frame = 3
Query: 951 HRLIGEEQELFFFHN----SSPGSAFMLPRGTHICNKLIDLIREQYRKRGFSEVTTPQIFKNELWETSGHWDHYQENLFKISLKSKEEFSIKPMN-CPAHCLIYSHKQRYSKDLPLRLADFGALHRFELTGELSGLTRLRKFHQDDAHIF-CRPDQIEQEIERSLDFIKHVYGILNFEF 1469
++ + + Q+L + N SS G+ ++P +KLI LI ++ ++T P + ++ +W+ SG WD + L+K + +E ++P + L+ + + +KD+P+ L R E+ + GL R R+F D + F C ER + +++ L+ +F
Sbjct: 21 NKYLSKSQKLLLYCNLISPSSFGTYTIMPILQRSLDKLIVLINKEMEFINAQKITMPSLGRSSIWKQSGRWDLLGKELYKFMDRDSKEMCLQPTHEEEVTSLLSTFQPLSTKDMPILLYQITPKFRNEVAPKY-GLLRGREFLMKDLYSFDCDESSAMLTYERVVQQYHNLFQQLDVKF 198 The following BLAST results are available for this feature:
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 4
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Threonine--tRNA ligase, cytoplasmic vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Threonine--tRNA ligase, cytoplasmic vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Threonine--tRNA ligase, cytoplasmic vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035804 ID=SMED30035804|Name=Threonine--tRNA ligase, cytoplasmic|organism=Schmidtea mediterranea sexual|type=transcript|length=2161bpback to top protein sequence of SMED30035804-orf-1 >SMED30035804-orf-1 ID=SMED30035804-orf-1|Name=SMED30035804-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=557bp MILRKIHYFSFVNKLILNKFLKRSLSENELLKVQQIKQFLWKDLLDKNTKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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