SMED30035773

Overview


Name	SMED30035773
Smed ID	SMED30035773
Length (bp)	602

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30035773 (SMED30035773) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30035773 (SMED30035773) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30035773 (SMED30035773) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30035773 (SMED30035773) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30035773

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
cephalic ganglia	SMED30035773	SMESG000017090.1	dd_Smed_v4_6825_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30035773	SMESG000017090.1	dd_Smed_v4_6825_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30035773	SMESG000017090.1	dd_Smed_v4_6825_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30035773 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.001809:47980..53663 -	2752

Homology

[Close]

BLAST of SMED30035773 vs. Ensembl Human
Match: TRPM5 (transient receptor potential cation channel subfamily M member 5 [Source:HGNC Symbol;Acc:HGNC:14323])

HSP 1 Score: 56.6102 bits (135), Expect = 5.271e-9
Identity = 45/152 (29.61%), Postives = 74/152 (48.68%), Query Frame = -1

Query:  144 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESY--TNLNCSSTSCRIRDPVVLVAL----------GLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGKEVLHLRRNI 563
            G   QAL HP       I   +L++PY  +FG + L+      +NCS+    + D     +L            FLL+T++LL+NLLIAMF +TF  +Q +    +   +  L+ E++E+  + PP   +    L +R +  KE  H R ++
Sbjct:  882 GVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSTHPLLLEDSPSCPSLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYNLIVEYHERPALAPPFILLSHLSLTLRRVFKKEAEHKREHL 1033

[Close]

BLAST of SMED30035773 vs. Ensembl Human
Match: TRPM5 (transient receptor potential cation channel subfamily M member 5 [Source:HGNC Symbol;Acc:HGNC:14323])

HSP 1 Score: 56.6102 bits (135), Expect = 5.274e-9
Identity = 45/152 (29.61%), Postives = 74/152 (48.68%), Query Frame = -1

Query:  144 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESY--TNLNCSSTSCRIRDPVVLVAL----------GLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGKEVLHLRRNI 563
            G   QAL HP       I   +L++PY  +FG + L+      +NCS+    + D     +L            FLL+T++LL+NLLIAMF +TF  +Q +    +   +  L+ E++E+  + PP   +    L +R +  KE  H R ++
Sbjct:  888 GVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSTHPLLLEDSPSCPSLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYNLIVEYHERPALAPPFILLSHLSLTLRRVFKKEAEHKREHL 1039

[Close]

BLAST of SMED30035773 vs. Ensembl Human
Match: TRPM5 (transient receptor potential cation channel subfamily M member 5 [Source:HGNC Symbol;Acc:HGNC:14323])

HSP 1 Score: 56.6102 bits (135), Expect = 5.275e-9
Identity = 45/152 (29.61%), Postives = 74/152 (48.68%), Query Frame = -1

Query:  144 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESY--TNLNCSSTSCRIRDPVVLVAL----------GLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGKEVLHLRRNI 563
            G   QAL HP       I   +L++PY  +FG + L+      +NCS+    + D     +L            FLL+T++LL+NLLIAMF +TF  +Q +    +   +  L+ E++E+  + PP   +    L +R +  KE  H R ++
Sbjct:  888 GVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSTHPLLLEDSPSCPSLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYNLIVEYHERPALAPPFILLSHLSLTLRRVFKKEAEHKREHL 1039

[Close]

BLAST of SMED30035773 vs. Ensembl Human
Match: TRPM5 (transient receptor potential cation channel subfamily M member 5 [Source:HGNC Symbol;Acc:HGNC:14323])

HSP 1 Score: 56.6102 bits (135), Expect = 5.278e-9
Identity = 45/152 (29.61%), Postives = 74/152 (48.68%), Query Frame = -1

Query:  144 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESY--TNLNCSSTSCRIRDPVVLVAL----------GLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGKEVLHLRRNI 563
            G   QAL HP       I   +L++PY  +FG + L+      +NCS+    + D     +L            FLL+T++LL+NLLIAMF +TF  +Q +    +   +  L+ E++E+  + PP   +    L +R +  KE  H R ++
Sbjct:  888 GVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSTHPLLLEDSPSCPSLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYNLIVEYHERPALAPPFILLSHLSLTLRRVFKKEAEHKREHL 1039

[Close]

BLAST of SMED30035773 vs. Ensembl Human
Match: TRPM3 (transient receptor potential cation channel subfamily M member 3 [Source:HGNC Symbol;Acc:HGNC:17992])

HSP 1 Score: 50.8322 bits (120), Expect = 5.833e-7
Identity = 30/120 (25.00%), Postives = 58/120 (48.33%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSST------------SCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++ +  +I + PY+ ++G++  +   +  C                C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct: 1023 GVARQAILFPNEEPSWKLAKNIFYMPYWMIYGEVFADQ-IDPPCGQNETREDGKIIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1141

[Close]

BLAST of SMED30035773 vs. Ensembl Fly
Match: Trpm (gene:FBgn0265194 transcript:FBtr0339075)

HSP 1 Score: 64.3142 bits (155), Expect = 5.942e-12
Identity = 38/134 (28.36%), Postives = 73/134 (54.48%), Query Frame = -1

Query:  171 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLM---LESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGK 563
            G   QA+ +P    T+S+I ++ FQPYF ++G++    ++     + S   C     V  + + ++LL+ +ILL+NLLIA+F + FNE+       +   +  ++ E+ +K  + PP   +  FY +++Y + K
Sbjct:  975 GVSRQAILYPNKQPTWSLIKEVTFQPYFMLYGEVFAGDIDPPCGEDPSQPGCVTGHWVTPITMSMYLLIANILLINLLIAVFNNIFNEVNSVSHQVWMFQRFTVVMEYQQKPVLPPPFIALCHFYSLLKYCVRK 1108

[Close]

BLAST of SMED30035773 vs. Ensembl Fly
Match: Trpm (gene:FBgn0265194 transcript:FBtr0339082)

HSP 1 Score: 63.1586 bits (152), Expect = 1.943e-11
Identity = 38/134 (28.36%), Postives = 73/134 (54.48%), Query Frame = -1

Query:  171 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLM---LESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGK 563
            G   QA+ +P    T+S+I ++ FQPYF ++G++    ++     + S   C     V  + + ++LL+ +ILL+NLLIA+F + FNE+       +   +  ++ E+ +K  + PP   +  FY +++Y + K
Sbjct:  956 GVSRQAILYPNKQPTWSLIKEVTFQPYFMLYGEVFAGDIDPPCGEDPSQPGCVTGHWVTPITMSMYLLIANILLINLLIAVFNNIFNEVNSVSHQVWMFQRFTVVMEYQQKPVLPPPFIALCHFYSLLKYCVRK 1089

[Close]

BLAST of SMED30035773 vs. Ensembl Fly
Match: Trpm (gene:FBgn0265194 transcript:FBtr0339080)

HSP 1 Score: 63.1586 bits (152), Expect = 1.951e-11
Identity = 38/134 (28.36%), Postives = 73/134 (54.48%), Query Frame = -1

Query:  171 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLM---LESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGK 563
            G   QA+ +P    T+S+I ++ FQPYF ++G++    ++     + S   C     V  + + ++LL+ +ILL+NLLIA+F + FNE+       +   +  ++ E+ +K  + PP   +  FY +++Y + K
Sbjct: 1022 GVSRQAILYPNKQPTWSLIKEVTFQPYFMLYGEVFAGDIDPPCGEDPSQPGCVTGHWVTPITMSMYLLIANILLINLLIAVFNNIFNEVNSVSHQVWMFQRFTVVMEYQQKPVLPPPFIALCHFYSLLKYCVRK 1155

[Close]

BLAST of SMED30035773 vs. Ensembl Fly
Match: Trpm (gene:FBgn0265194 transcript:FBtr0339076)

HSP 1 Score: 62.7734 bits (151), Expect = 2.043e-11
Identity = 38/134 (28.36%), Postives = 73/134 (54.48%), Query Frame = -1

Query:  171 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLM---LESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGK 563
            G   QA+ +P    T+S+I ++ FQPYF ++G++    ++     + S   C     V  + + ++LL+ +ILL+NLLIA+F + FNE+       +   +  ++ E+ +K  + PP   +  FY +++Y + K
Sbjct:  975 GVSRQAILYPNKQPTWSLIKEVTFQPYFMLYGEVFAGDIDPPCGEDPSQPGCVTGHWVTPITMSMYLLIANILLINLLIAVFNNIFNEVNSVSHQVWMFQRFTVVMEYQQKPVLPPPFIALCHFYSLLKYCVRK 1108

[Close]

BLAST of SMED30035773 vs. Ensembl Fly
Match: Trpm (gene:FBgn0265194 transcript:FBtr0339079)

HSP 1 Score: 62.7734 bits (151), Expect = 2.102e-11
Identity = 38/134 (28.36%), Postives = 73/134 (54.48%), Query Frame = -1

Query:  171 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLM---LESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGK 563
            G   QA+ +P    T+S+I ++ FQPYF ++G++    ++     + S   C     V  + + ++LL+ +ILL+NLLIA+F + FNE+       +   +  ++ E+ +K  + PP   +  FY +++Y + K
Sbjct:  982 GVSRQAILYPNKQPTWSLIKEVTFQPYFMLYGEVFAGDIDPPCGEDPSQPGCVTGHWVTPITMSMYLLIANILLINLLIAVFNNIFNEVNSVSHQVWMFQRFTVVMEYQQKPVLPPPFIALCHFYSLLKYCVRK 1115

[Close]

BLAST of SMED30035773 vs. Ensembl Zebrafish
Match: hrc (histidine rich calcium binding protein [Source:ZFIN;Acc:ZDB-GENE-041008-189])

HSP 1 Score: 57.7658 bits (138), Expect = 1.142e-9
Identity = 36/124 (29.03%), Postives = 64/124 (51.61%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLE--------------SYTNLNCSSTSCR--IRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QAL +        +   +L++PY H+FGD+ +E              + T +N     CR    + +V++ L +FLL+T+ILL+NLLIA F +TF ++Q     ++   +  L+ E++ +
Sbjct:  162 GVANQALLYQYDPSPARVFRRVLYRPYLHIFGDIPVEEIDTGKHWERDCTDNLTLINEGIEPCRDTSHNWLVVILLVIFLLVTNILLINLLIATFSYTFTKVQEHSDKYWKFQRYNLIVEYHSR 285

[Close]

BLAST of SMED30035773 vs. Ensembl Zebrafish
Match: hrc (histidine rich calcium binding protein [Source:ZFIN;Acc:ZDB-GENE-041008-189])

HSP 1 Score: 56.6102 bits (135), Expect = 3.504e-9
Identity = 36/124 (29.03%), Postives = 64/124 (51.61%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLE--------------SYTNLNCSSTSCR--IRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QAL +        +   +L++PY H+FGD+ +E              + T +N     CR    + +V++ L +FLL+T+ILL+NLLIA F +TF ++Q     ++   +  L+ E++ +
Sbjct:  965 GVANQALLYQYDPSPARVFRRVLYRPYLHIFGDIPVEEIDTGKHWERDCTDNLTLINEGIEPCRDTSHNWLVVILLVIFLLVTNILLINLLIATFSYTFTKVQEHSDKYWKFQRYNLIVEYHSR 1088

[Close]

BLAST of SMED30035773 vs. Ensembl Zebrafish
Match: trpm4a (transient receptor potential cation channel, subfamily M, member 4a [Source:ZFIN;Acc:ZDB-GENE-090302-3])

HSP 1 Score: 55.4546 bits (132), Expect = 9.895e-9
Identity = 35/124 (28.23%), Postives = 64/124 (51.61%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLE--------------SYTNLNCSSTSCRIRDP--VVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QAL +        +   + ++PY H++G + L+              + T ++  +  C   D   +VL+ L ++LL+T+ILLVNLLIAMF +TFN++Q     ++   +  L+ E++ +
Sbjct:  993 GVANQALLYSYDSRPGWVARRVFYRPYMHIYGQIPLDEIDADKRHDKRCTDNVTLIHEGAEPCPDSDANWLVLILLSVYLLVTNILLVNLLIAMFSYTFNKVQERSDVYWKFQRYNLIVEYHSR 1116

[Close]

BLAST of SMED30035773 vs. Ensembl Zebrafish
Match: trpm3 (transient receptor potential cation channel, subfamily M, member 3 [Source:ZFIN;Acc:ZDB-GENE-060531-95])

HSP 1 Score: 51.2174 bits (121), Expect = 2.611e-7
Identity = 31/119 (26.05%), Postives = 61/119 (51.26%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDL---MLESYTNLNCSS--------TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+ +P    ++ +  +I F PY+ ++G++    ++     N ++          C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct: 1034 GVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1152

[Close]

BLAST of SMED30035773 vs. Ensembl Zebrafish
Match: trpm3 (transient receptor potential cation channel, subfamily M, member 3 [Source:ZFIN;Acc:ZDB-GENE-060531-95])

HSP 1 Score: 51.2174 bits (121), Expect = 2.847e-7
Identity = 31/119 (26.05%), Postives = 61/119 (51.26%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDL---MLESYTNLNCSS--------TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+ +P    ++ +  +I F PY+ ++G++    ++     N ++          C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct:  883 GVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1001

[Close]

BLAST of SMED30035773 vs. Ensembl Xenopus
Match: aldh3a2 (aldehyde dehydrogenase 3 family member A2 [Source:Xenbase;Acc:XB-GENE-942662])

HSP 1 Score: 51.9878 bits (123), Expect = 2.233e-7
Identity = 30/119 (25.21%), Postives = 60/119 (50.42%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDL---MLESYTNLNCSS--------TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++++  +I + PY+ ++G++    ++     N +           C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct:  998 GVARQAILFPSEEPSWNLAKNIFYMPYWMIYGEVFADQIDPPCGYNVTGEDGKIIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1116

[Close]

BLAST of SMED30035773 vs. Ensembl Xenopus
Match: aldh3a2 (aldehyde dehydrogenase 3 family member A2 [Source:Xenbase;Acc:XB-GENE-942662])

HSP 1 Score: 51.6026 bits (122), Expect = 3.141e-7
Identity = 30/119 (25.21%), Postives = 60/119 (50.42%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDL---MLESYTNLNCSS--------TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++++  +I + PY+ ++G++    ++     N +           C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct: 1027 GVARQAILFPSEEPSWNLAKNIFYMPYWMIYGEVFADQIDPPCGYNVTGEDGKIIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1145

[Close]

BLAST of SMED30035773 vs. Ensembl Xenopus
Match: aldh3a2 (aldehyde dehydrogenase 3 family member A2 [Source:Xenbase;Acc:XB-GENE-942662])

HSP 1 Score: 51.6026 bits (122), Expect = 3.165e-7
Identity = 30/119 (25.21%), Postives = 60/119 (50.42%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDL---MLESYTNLNCSS--------TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++++  +I + PY+ ++G++    ++     N +           C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct:  984 GVARQAILFPSEEPSWNLAKNIFYMPYWMIYGEVFADQIDPPCGYNVTGEDGKIIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1102

[Close]

BLAST of SMED30035773 vs. Ensembl Xenopus
Match: aldh3a2 (aldehyde dehydrogenase 3 family member A2 [Source:Xenbase;Acc:XB-GENE-942662])

HSP 1 Score: 51.2174 bits (121), Expect = 3.201e-7
Identity = 30/119 (25.21%), Postives = 60/119 (50.42%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDL---MLESYTNLNCSS--------TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++++  +I + PY+ ++G++    ++     N +           C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct:  840 GVARQAILFPSEEPSWNLAKNIFYMPYWMIYGEVFADQIDPPCGYNVTGEDGKIIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 958

[Close]

BLAST of SMED30035773 vs. Ensembl Xenopus
Match: aldh3a2 (aldehyde dehydrogenase 3 family member A2 [Source:Xenbase;Acc:XB-GENE-942662])

HSP 1 Score: 51.2174 bits (121), Expect = 3.319e-7
Identity = 30/119 (25.21%), Postives = 60/119 (50.42%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDL---MLESYTNLNCSS--------TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++++  +I + PY+ ++G++    ++     N +           C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct: 1004 GVARQAILFPSEEPSWNLAKNIFYMPYWMIYGEVFADQIDPPCGYNVTGEDGKIIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1122

[Close]

BLAST of SMED30035773 vs. Ensembl Mouse
Match: Trpm3 (transient receptor potential cation channel, subfamily M, member 3 [Source:MGI Symbol;Acc:MGI:2443101])

HSP 1 Score: 51.2174 bits (121), Expect = 2.393e-7
Identity = 30/120 (25.00%), Postives = 58/120 (48.33%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSST------------SCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++ +  +I + PY+ ++G++  +   +  C                C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct:  546 GVARQAILFPNEEPSWKLAKNIFYMPYWMIYGEVFADQ-IDPPCGQNETREDGKTIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 664

[Close]

BLAST of SMED30035773 vs. Ensembl Mouse
Match: Trpm5 (transient receptor potential cation channel, subfamily M, member 5 [Source:MGI Symbol;Acc:MGI:1861718])

HSP 1 Score: 51.2174 bits (121), Expect = 2.568e-7
Identity = 46/152 (30.26%), Postives = 79/152 (51.97%), Query Frame = -1

Query:  144 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLES--YTNLNCS--------STSCR--IRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGKEVLHLRRNI 563
            G   QAL HP       I   +L++PY  +FG + L+      +NCS        S SC     + +V++ L  FLL+T++LL+NLLIAMF +TF  +Q +    +   +  L+ E++ +  + PP   +    L+++ +  KE  H R+++
Sbjct:  887 GVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLESSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKRQHL 1038

[Close]

BLAST of SMED30035773 vs. Ensembl Mouse
Match: Trpm3 (transient receptor potential cation channel, subfamily M, member 3 [Source:MGI Symbol;Acc:MGI:2443101])

HSP 1 Score: 50.8322 bits (120), Expect = 4.008e-7
Identity = 30/120 (25.00%), Postives = 58/120 (48.33%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSST------------SCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++ +  +I + PY+ ++G++  +   +  C                C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct: 1035 GVARQAILFPNEEPSWKLAKNIFYMPYWMIYGEVFADQ-IDPPCGQNETREDGKTIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1153

[Close]

BLAST of SMED30035773 vs. Ensembl Mouse
Match: Trpm3 (transient receptor potential cation channel, subfamily M, member 3 [Source:MGI Symbol;Acc:MGI:2443101])

HSP 1 Score: 50.0618 bits (118), Expect = 6.834e-7
Identity = 30/120 (25.00%), Postives = 58/120 (48.33%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSST------------SCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++ +  +I + PY+ ++G++  +   +  C                C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct: 1007 GVARQAILFPNEEPSWKLAKNIFYMPYWMIYGEVFADQ-IDPPCGQNETREDGKTIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1125

[Close]

BLAST of SMED30035773 vs. Ensembl Mouse
Match: Trpm3 (transient receptor potential cation channel, subfamily M, member 3 [Source:MGI Symbol;Acc:MGI:2443101])

HSP 1 Score: 50.0618 bits (118), Expect = 6.834e-7
Identity = 30/120 (25.00%), Postives = 58/120 (48.33%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSST------------SCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++ +  +I + PY+ ++G++  +   +  C                C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct: 1008 GVARQAILFPNEEPSWKLAKNIFYMPYWMIYGEVFADQ-IDPPCGQNETREDGKTIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1126

[Close]

BLAST of SMED30035773 vs. UniProt/SwissProt
Match: sp|A8DYE2|TRPCG_DROME (Transient receptor potential cation channel trpm OS=Drosophila melanogaster OX=7227 GN=Trpm PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.237e-10
Identity = 37/130 (28.46%), Postives = 72/130 (55.38%), Query Frame = -1

Query:  171 QALQHPXSHLTYSIINDILFQPYFHVFGDLM---LESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGK 551
            QA+ +P    T+S+I ++ FQPYF ++G++    ++     + S   C     V  + + ++LL+ +ILL+NLLIA+F + FNE+       +   +  ++ E+ +K  + PP   +  FY +++Y + K
Sbjct: 1081 QAILYPNKQPTWSLIKEVTFQPYFMLYGEVFAGDIDPPCGEDPSQPGCVTGHWVTPITMSMYLLIANILLINLLIAVFNNIFNEVNSVSHQVWMFQRFTVVMEYQQKPVLPPPFIALCHFYSLLKYCVRK 1210

[Close]

BLAST of SMED30035773 vs. UniProt/SwissProt
Match: sp|Q9NZQ8|TRPM5_HUMAN (Transient receptor potential cation channel subfamily M member 5 OS=Homo sapiens OX=9606 GN=TRPM5 PE=1 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 2.533e-8
Identity = 45/152 (29.61%), Postives = 74/152 (48.68%), Query Frame = -1

Query:  144 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESY--TNLNCSSTSCRIRDPVVLVAL----------GLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGKEVLHLRRNI 563
            G   QAL HP       I   +L++PY  +FG + L+      +NCS+    + D     +L            FLL+T++LL+NLLIAMF +TF  +Q +    +   +  L+ E++E+  + PP   +    L +R +  KE  H R ++
Sbjct:  888 GVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSTHPLLLEDSPSCPSLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYNLIVEYHERPALAPPFILLSHLSLTLRRVFKKEAEHKREHL 1039

[Close]

BLAST of SMED30035773 vs. UniProt/SwissProt
Match: sp|Q9JJH7|TRPM5_MOUSE (Transient receptor potential cation channel subfamily M member 5 OS=Mus musculus OX=10090 GN=Trpm5 PE=1 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 1.796e-6
Identity = 46/152 (30.26%), Postives = 79/152 (51.97%), Query Frame = -1

Query:  144 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLES--YTNLNCS--------STSCR--IRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGKEVLHLRRNI 563
            G   QAL HP       I   +L++PY  +FG + L+      +NCS        S SC     + +V++ L  FLL+T++LL+NLLIAMF +TF  +Q +    +   +  L+ E++ +  + PP   +    L+++ +  KE  H R+++
Sbjct:  887 GVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLESSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKRQHL 1038

[Close]

BLAST of SMED30035773 vs. UniProt/SwissProt
Match: sp|Q9HCF6|TRPM3_HUMAN (Transient receptor potential cation channel subfamily M member 3 OS=Homo sapiens OX=9606 GN=TRPM3 PE=2 SV=4)

HSP 1 Score: 50.0618 bits (118), Expect = 4.613e-6
Identity = 30/120 (25.00%), Postives = 58/120 (48.33%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSST------------SCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+  P    ++ +  +I + PY+ ++G++  +   +  C                C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct: 1048 GVARQAILFPNEEPSWKLAKNIFYMPYWMIYGEVFADQ-IDPPCGQNETREDGKIIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1166

[Close]

BLAST of SMED30035773 vs. TrEMBL
Match: R7U3A5 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_220620 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.648e-10
Identity = 43/140 (30.71%), Postives = 75/140 (53.57%), Query Frame = -1

Query:  177 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLN-CSS----------TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYII 563
            G   QA+ +P + L + +I D+L   Y+ ++G+L LE     N C++            C     V  V LG ++L+T++L++NLLIAMF +TFN +Q     ++   +  + +E+Y++  + PP   I   YL+ R +I
Sbjct: 1035 GVASQAILYPNNPLRWELIRDVLHMSYWQMYGELFLEDIEGSNECTNDIAVWSAGTMARCPSNSWVASVLLGFYILITNVLMLNLLIAMFSNTFNAVQEKTDMYWCFQRYTMTKEYYQRPVLAPPFVVISHAYLLFRMVI 1174

[Close]

BLAST of SMED30035773 vs. TrEMBL
Match: A0A267EVM8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig014651g1 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 5.353e-10
Identity = 32/93 (34.41%), Postives = 61/93 (65.59%), Query Frame = -1

Query:  240 YSIINDILFQPYFHVFGDLMLESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 518
            + I+  +L+ PY+ ++G+L LES         S   R+ +V+V +  ++L T++LL+NLLIAMFGHTF ++Q +   +++ ++ +L+ E+Y +
Sbjct: 1215 HDIMRGVLYIPYWQMYGELELESVDQGMAVGGS---RNWLVIVLMAFYMLGTNVLLLNLLIAMFGHTFEKVQSESNKYYAYIRHELIYEYYTR 1304

[Close]

BLAST of SMED30035773 vs. TrEMBL
Match: K1QMV6 (Transient receptor potential cation channel subfamily M member 3 OS=Crassostrea gigas OX=29159 GN=CGI_10019312 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 7.316e-10
Identity = 41/146 (28.08%), Postives = 81/146 (55.48%), Query Frame = -1

Query:  177 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSSTS-----------------CRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYII 563
            G   QA  +P +   ++++ D+L+ PY+ ++G+L LE+    + S  +                 C  ++ +V++ +  ++L+T+ILLVNLLIAMF  TF ++Q +    +   +  L+ E+YE+  +FPP+  ++  Y  +R++I
Sbjct: 1160 GVAYQANLYPNAPQQWTVLKDVLYHPYWQMYGELFLENKEGNDPSEEAGTCTNNETLWRSGEMDRCPEKNAMVVLLMVTYMLLTNILLVNLLIAMFSDTFQKVQDNSEKVWRFHRFSLVYEYYERPALFPPLIILNHMYRAIRWLI 1305

[Close]

BLAST of SMED30035773 vs. TrEMBL
Match: A0A3L5TUY4 (Ion_trans domain-containing protein (Fragment) OS=Mytilus galloprovincialis OX=29158 GN=AM593_06867 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.891e-10
Identity = 32/121 (26.45%), Postives = 66/121 (54.55%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNL-------------NCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G + QA  +P +   + +  ++++ PY+ ++G+L LE +                N +   C +R+ +    L +++++T I+LVNLLIAMF HTF ++Q +    +   +  L++E+Y++
Sbjct:   53 GVIYQATMYPNTEPGFPLFQNLVYMPYWQLYGELFLEQFYGALPDDCTENVELYSNGTMDRCPLRNQINTFVLSIYMVVTHIVLVNLLIAMFSHTFTKVQDNNELVWKFHRFSLIQEYYDR 173

[Close]

BLAST of SMED30035773 vs. TrEMBL
Match: A0A369RXU4 (Transient receptor potential cation channel subfamily M member 2 OS=Trichoplax sp. H2 OX=287889 GN=TrispH2_009492 PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 2.083e-9
Identity = 39/116 (33.62%), Postives = 58/116 (50.00%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSSTSCRIRDPVVLVA--------LGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G    A+ HP      +II  I + PYF+++G+L L      NC        DP   VA          L+LL+T+ILL+NLLIAMF +TF+ +Q      +   +  L+ E+ E+
Sbjct: 1006 GVSSHAILHPNYPFNATIIQKIFYIPYFNIYGELFLAEIQGQNCPPGQNYTTDPTNCVANNWLVPVLFSLYLLITNILLINLLIAMFNNTFSLVQERSDLVWKFQRYDLIREYLER 1121

[Close]

BLAST of SMED30035773 vs. Ensembl Cavefish
Match: hrc (transient receptor potential cation channel subfamily M member 4-like [Source:NCBI gene;Acc:103047479])

HSP 1 Score: 53.1434 bits (126), Expect = 4.132e-8
Identity = 31/107 (28.97%), Postives = 60/107 (56.07%), Query Frame = -1

Query:  240 IINDILFQPYFHVFGDLMLESYT-----NLNCSSTSCRI-------RDP----VVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 512
            I+  + ++PY H+FG +  E        N+ C++    I       RD     +V++ L ++LL+T+ILL+NLLIAMF +TF ++Q     ++   + +++ E++ +
Sbjct:  993 ILRRVFYRPYLHIFGQIPYEQMDSGFEWNVTCTTNVTEIDENMEPCRDTRYNWMVVILLMIYLLVTNILLINLLIAMFSYTFTKVQERSDVYWKFQRFKVIIEYHSR 1099

[Close]

BLAST of SMED30035773 vs. Ensembl Cavefish
Match: trpm3 (transient receptor potential cation channel, subfamily M, member 3 [Source:ZFIN;Acc:ZDB-GENE-060531-95])

HSP 1 Score: 51.2174 bits (121), Expect = 1.890e-7
Identity = 30/112 (26.79%), Postives = 56/112 (50.00%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSS----TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+ +P    ++ +  +I F PY+ ++G+            +      C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct:  870 GVARQAILNPNEDPSWMLARNIFFMPYWMIYGEAPCGQNITTEDGTIVPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 981

[Close]

BLAST of SMED30035773 vs. Ensembl Medaka
Match: hrc (transient receptor potential cation channel subfamily M member 4 [Source:NCBI gene;Acc:101158642])

HSP 1 Score: 58.9214 bits (141), Expect = 5.125e-10
Identity = 35/105 (33.33%), Postives = 58/105 (55.24%), Query Frame = -1

Query:  297 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLE--------------SYTNLNCSSTSCRIR--DPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRD 563
            G   QAL +        I   + ++PY H+FG + +E              + T ++  S  CR+   + VV+V L ++LL+T+I+L+NLLIA+F HTF+E+Q +
Sbjct:  993 GVANQALIYSYDSNPDRIFRRVFYRPYLHIFGQIPVEEIDSGKNWDLECTNNMTMIDSGSEPCRVEHSNWVVVVLLIIYLLVTNIVLINLLIAIFSHTFSEVQAN 1097

[Close]

BLAST of SMED30035773 vs. Ensembl Medaka
Match: ENSORLT00000000453.2 (pep primary_assembly:ASM223467v1:3:5283852:5304338:1 gene:ENSORLG00000000365.2 transcript:ENSORLT00000000453.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 7.873e-8
Identity = 31/117 (26.50%), Postives = 57/117 (48.72%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLES---------YTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+ HP    T+ +  +I + PY+ ++G++  +S         Y         C     +    +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+++
Sbjct:  510 GVARQAILHPDEEPTWRLARNIFYMPYWMIYGEVFADSIDPPCGENMYDEDGKKLPPCIPGAWLTPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDR 626

[Close]

BLAST of SMED30035773 vs. Ensembl Medaka
Match: trpm3 (transient receptor potential cation channel subfamily M member 3 [Source:NCBI gene;Acc:100533512])

HSP 1 Score: 51.6026 bits (122), Expect = 1.541e-7
Identity = 31/119 (26.05%), Postives = 62/119 (52.10%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDL---MLESYTNLNCSS--------TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+ +P    ++ +  +I F PY+ ++G++    ++     N ++         +C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct:  995 GVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTDDGVVVALPACKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1113

[Close]

BLAST of SMED30035773 vs. Ensembl Medaka
Match: trpm3 (transient receptor potential cation channel subfamily M member 3 [Source:NCBI gene;Acc:100533512])

HSP 1 Score: 51.6026 bits (122), Expect = 1.569e-7
Identity = 31/119 (26.05%), Postives = 62/119 (52.10%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDL---MLESYTNLNCSS--------TSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G   QA+ +P    ++ +  +I F PY+ ++G++    ++     N ++         +C+    +V   +  +LL+ +ILLVNLLIA+F +TF E++      +   + QL+  F+E+
Sbjct:  995 GVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTDDGVVVALPACKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHER 1113

[Close]

BLAST of SMED30035773 vs. Planmine SMEST
Match: SMESG000017090.1 (SMESG000017090.1)

HSP 1 Score: 315.849 bits (808), Expect = 5.534e-102
Identity = 166/169 (98.22%), Postives = 167/169 (98.82%), Query Frame = -1

Query:   57 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVRYIIGKEVLHLRRNILVSRATCDKSKMVEXEKMFLTSVIRKFRL 563
            GSLVQALQHP SHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKIFIFPPIFFIDLFYLIVRYIIGKEVLHLRRNILVSRATCDKSKMVE EKMFLT+VIRKFRL
Sbjct:  814 GSLVQALQHPNSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKIFIFPPIFFIDLFYLIVRYIIGKEVLHLRRNILVSRATCDKSKMVELEKMFLTTVIRKFRL 982

[Close]

BLAST of SMED30035773 vs. Planmine SMEST
Match: SMESG000005058.1 (SMESG000005058.1)

HSP 1 Score: 108.227 bits (269), Expect = 2.040e-27
Identity = 56/143 (39.16%), Postives = 88/143 (61.54%), Query Frame = -1

Query:  144 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVR---YIIGKEVLHLRRNI 563
            G+L Q+L+ P S    ++  DI++ PYF  FG+LMLE + +  C+ T C IR+  V+ +  ++LLM  ILLVNLLIAMFG+TF +I  D    +  +++ L++EFYEK         +++FY I+R   Y++     H +RN+
Sbjct:  221 GTLFQSLKFPNSKFDLNLFKDIVYLPYFQTFGELMLEIFDSGTCNRTDCVIRNATVIFSFAIYLLMAHILLVNLLIAMFGYTFQQIHEDSEKLWCFIRVMLVQEFYEK---NILPPPLNIFYCIIRLFNYLLPN---HFKRNL 357

[Close]

BLAST of SMED30035773 vs. Planmine SMEST
Match: SMESG000005058.1 (SMESG000005058.1)

HSP 1 Score: 108.227 bits (269), Expect = 2.122e-27
Identity = 56/143 (39.16%), Postives = 88/143 (61.54%), Query Frame = -1

Query:  144 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVR---YIIGKEVLHLRRNI 563
            G+L Q+L+ P S    ++  DI++ PYF  FG+LMLE + +  C+ T C IR+  V+ +  ++LLM  ILLVNLLIAMFG+TF +I  D    +  +++ L++EFYEK         +++FY I+R   Y++     H +RN+
Sbjct:  218 GTLFQSLKFPNSKFDLNLFKDIVYLPYFQTFGELMLEIFDSGTCNRTDCVIRNATVIFSFAIYLLMAHILLVNLLIAMFGYTFQQIHEDSEKLWCFIRVMLVQEFYEK---NILPPPLNIFYCIIRLFNYLLPN---HFKRNL 354

[Close]

BLAST of SMED30035773 vs. Planmine SMEST
Match: SMESG000005058.1 (SMESG000005058.1)

HSP 1 Score: 107.842 bits (268), Expect = 2.178e-27
Identity = 56/143 (39.16%), Postives = 88/143 (61.54%), Query Frame = -1

Query:  144 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEKXXXXXXXXXIDLFYLIVR---YIIGKEVLHLRRNI 563
            G+L Q+L+ P S    ++  DI++ PYF  FG+LMLE + +  C+ T C IR+  V+ +  ++LLM  ILLVNLLIAMFG+TF +I  D    +  +++ L++EFYEK         +++FY I+R   Y++     H +RN+
Sbjct:  216 GTLFQSLKFPNSKFDLNLFKDIVYLPYFQTFGELMLEIFDSGTCNRTDCVIRNATVIFSFAIYLLMAHILLVNLLIAMFGYTFQQIHEDSEKLWCFIRVMLVQEFYEK---NILPPPLNIFYCIIRLFNYLLPN---HFKRNL 352

[Close]

BLAST of SMED30035773 vs. Planmine SMEST
Match: SMESG000005057.1 (SMESG000005057.1)

HSP 1 Score: 103.605 bits (257), Expect = 1.425e-25
Identity = 47/109 (43.12%), Postives = 74/109 (67.89%), Query Frame = -1

Query:  240 GSLVQALQHPXSHLTYSIINDILFQPYFHVFGDLMLESYTNLN-CSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFNEIQRDVADHFSILQLQLLEEFYEK 563
            G L ++L++P S     +  DI++ P+F  FG+LMLE++     C+ T C +    VL++  +FLLMT++LL+NLLIAMFG+TFN+IQ D    +  +++QL++EFY K
Sbjct:  946 GILARSLKYPNSEFNTLLFKDIVYTPFFQTFGELMLENFDVAGPCNGTDCIVSSGTVLISFAVFLLMTNVLLINLLIAMFGYTFNQIQEDSEKLWCFIRIQLVQEFYGK 1054

The following BLAST results are available for this feature:

BLAST of SMED30035773 vs. Ensembl Human
BLAST of SMED30035773 vs. Ensembl Celegans
BLAST of SMED30035773 vs. Ensembl Fly
BLAST of SMED30035773 vs. Ensembl Zebrafish
BLAST of SMED30035773 vs. Ensembl Xenopus
BLAST of SMED30035773 vs. Ensembl Mouse
BLAST of SMED30035773 vs. UniProt/SwissProt
BLAST of SMED30035773 vs. TrEMBL
BLAST of SMED30035773 vs. Ensembl Cavefish
BLAST of SMED30035773 vs. Ensembl Sea Lamprey
BLAST of SMED30035773 vs. Ensembl Yeast
BLAST of SMED30035773 vs. Ensembl Nematostella
BLAST of SMED30035773 vs. Ensembl Medaka
BLAST of SMED30035773 vs. Planmine SMEST

BLAST of SMED30035773 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
TRPM5	5.271e-9	29.61	transient receptor potential cation channel subfam...	[more]
TRPM5	5.274e-9	29.61	transient receptor potential cation channel subfam...	[more]
TRPM5	5.275e-9	29.61	transient receptor potential cation channel subfam...	[more]
TRPM5	5.278e-9	29.61	transient receptor potential cation channel subfam...	[more]
TRPM3	5.833e-7	25.00	transient receptor potential cation channel subfam...	[more]

BLAST of SMED30035773 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30035773 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Trpm	5.942e-12	28.36	gene:FBgn0265194 transcript:FBtr0339075	[more]
Trpm	1.943e-11	28.36	gene:FBgn0265194 transcript:FBtr0339082	[more]
Trpm	1.951e-11	28.36	gene:FBgn0265194 transcript:FBtr0339080	[more]
Trpm	2.043e-11	28.36	gene:FBgn0265194 transcript:FBtr0339076	[more]
Trpm	2.102e-11	28.36	gene:FBgn0265194 transcript:FBtr0339079	[more]

BLAST of SMED30035773 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
hrc	1.142e-9	29.03	histidine rich calcium binding protein [Source:ZFI...	[more]
hrc	3.504e-9	29.03	histidine rich calcium binding protein [Source:ZFI...	[more]
trpm4a	9.895e-9	28.23	transient receptor potential cation channel, subfa...	[more]
trpm3	2.611e-7	26.05	transient receptor potential cation channel, subfa...	[more]
trpm3	2.847e-7	26.05	transient receptor potential cation channel, subfa...	[more]

BLAST of SMED30035773 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
aldh3a2	2.233e-7	25.21	aldehyde dehydrogenase 3 family member A2 [Source:...	[more]
aldh3a2	3.141e-7	25.21	aldehyde dehydrogenase 3 family member A2 [Source:...	[more]
aldh3a2	3.165e-7	25.21	aldehyde dehydrogenase 3 family member A2 [Source:...	[more]
aldh3a2	3.201e-7	25.21	aldehyde dehydrogenase 3 family member A2 [Source:...	[more]
aldh3a2	3.319e-7	25.21	aldehyde dehydrogenase 3 family member A2 [Source:...	[more]

BLAST of SMED30035773 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Trpm3	2.393e-7	25.00	transient receptor potential cation channel, subfa...	[more]
Trpm5	2.568e-7	30.26	transient receptor potential cation channel, subfa...	[more]
Trpm3	4.008e-7	25.00	transient receptor potential cation channel, subfa...	[more]
Trpm3	6.834e-7	25.00	transient receptor potential cation channel, subfa...	[more]
Trpm3	6.834e-7	25.00	transient receptor potential cation channel, subfa...	[more]

BLAST of SMED30035773 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4


Match Name	E-value	Identity	Description
sp\|A8DYE2\|TRPCG_DROME	2.237e-10	28.46	Transient receptor potential cation channel trpm O...	[more]
sp\|Q9NZQ8\|TRPM5_HUMAN	2.533e-8	29.61	Transient receptor potential cation channel subfam...	[more]
sp\|Q9JJH7\|TRPM5_MOUSE	1.796e-6	30.26	Transient receptor potential cation channel subfam...	[more]
sp\|Q9HCF6\|TRPM3_HUMAN	4.613e-6	25.00	Transient receptor potential cation channel subfam...	[more]

BLAST of SMED30035773 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
R7U3A5	1.648e-10	30.71	Uncharacterized protein OS=Capitella teleta OX=283...	[more]
A0A267EVM8	5.353e-10	34.41	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
K1QMV6	7.316e-10	28.08	Transient receptor potential cation channel subfam...	[more]
A0A3L5TUY4	7.891e-10	26.45	Ion_trans domain-containing protein (Fragment) OS=...	[more]
A0A369RXU4	2.083e-9	33.62	Transient receptor potential cation channel subfam...	[more]

BLAST of SMED30035773 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
hrc	4.132e-8	28.97	transient receptor potential cation channel subfam...	[more]
trpm3	1.890e-7	26.79	transient receptor potential cation channel, subfa...	[more]

BLAST of SMED30035773 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30035773 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30035773 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30035773 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4


Match Name	E-value	Identity	Description
hrc	5.125e-10	33.33	transient receptor potential cation channel subfam...	[more]
ENSORLT00000000453.2	7.873e-8	26.50	pep primary_assembly:ASM223467v1:3:5283852:5304338...	[more]
trpm3	1.541e-7	26.05	transient receptor potential cation channel subfam...	[more]
trpm3	1.569e-7	26.05	transient receptor potential cation channel subfam...	[more]

BLAST of SMED30035773 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000017090.1	5.534e-102	98.22	SMESG000017090.1	[more]
SMESG000005058.1	2.040e-27	39.16	SMESG000005058.1	[more]
SMESG000005058.1	2.122e-27	39.16	SMESG000005058.1	[more]
SMESG000005058.1	2.178e-27	39.16	SMESG000005058.1	[more]
SMESG000005057.1	1.425e-25	43.12	SMESG000005057.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30035773 ID=SMED30035773|Name=SMED30035773|organism=Schmidtea mediterranea sexual|type=transcript|length=602bp
TTTTTTTTTTTTTTTTTTTCCTTAATCCAATCACAATGTATTTAAATTTT
TTTCTTAAGTCTAAATTTTCGAATAACGGAAGTGAGAAACATTTTCTCGN
GTTCAACCATTTTTGATTTGTCGCAAGTTGCCCGACTTACTAGGATATTG
CGGCGCAAATGCAATACTTCTTTACCAATTATATATCTGACAATTAAATA
AAATAAATCTATGAAAAATATNGGAGGGAAAATAAATATTTTTTCATAGA
ATTCCTCTAGCAATTGCAGCTGCAAAATCGAAAAGTGATCAGCAACATCC
CTTTGAATCTCATTAAATGTATGTCCAAACATTGCAATCAGAAGATTTAC
AAGTAGAATTGATGTCATTAGCAAAAACAAGCCTAAAGCAACTAGCACCA
CTGGGTCACGAATTCGACAGCTCGTTGAACTACAATTCAGATTTGTATAA
CTCTCAAGCATTAGATCTCCGAAAACATGAAAATATGGCTGAAAAAGAAT
ATCATTAATGATGGAATAAGTTAAATGGCTNTTGGGATGTTGCAGTGCTT
GAACCAGTGATCCCGAAGGCTATGATAAATNTCAAAAAAATAAACAAAAA
GA

protein sequence of SMED30035773-orf-1

>SMED30035773-orf-1 ID=SMED30035773-orf-1|Name=SMED30035773-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=140bp
MLESYTNLNCSSTSCRIRDPVVLVALGLFLLMTSILLVNLLIAMFGHTFN
EIQRDVADHFSILQLQLLEEFYEKIFIFPXIFFIDLFYLIVRYIIGKEVL
HLRRNILVSRATCDKSKMVEXEKMFLTSVIRKFRLKKKI*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000101	muscle cell
PLANA:0003116	parenchymal cell

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SIGNALP_EUK	SignalP-TM	SignalP-TM	coord: 1..18 score: 0.56
None	No IPR available	TMHMM	TMhelix		coord: 2..19

Main menu

SMED30035773

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Owlery

blazegraph-runner