SMED30035747
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035747 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of SMED30035747 vs. Ensembl Human
Match: TIGD1 (tigger transposable element derived 1 [Source:HGNC Symbol;Acc:HGNC:14523]) HSP 1 Score: 54.299 bits (129), Expect = 7.286e-9 Identity = 34/107 (31.78%), Postives = 55/107 (51.40%), Query Frame = -1 Query: 120 TFSNLIAA-----TDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEILISHNEDL 425 TF +AA +D + +K FWK + I+ AIKNI SW E+K S + VW+ + + ++ V ++ E+A+E EV E + E+L SH++ L Sbjct: 350 TFHKALAAMDSDVSDGSGQSKLKTFWKGFTILDAIKNIRDSWEEVKLSTLTGVWKKLIPTLIDDYEGFKTSVEEVSADVVEIAKELELEVEPED-VTELLQSHDKTL 455
BLAST of SMED30035747 vs. UniProt/SwissProt
Match: sp|Q96MW7|TIGD1_HUMAN (Tigger transposable element-derived protein 1 OS=Homo sapiens OX=9606 GN=TIGD1 PE=1 SV=1) HSP 1 Score: 54.299 bits (129), Expect = 3.499e-8 Identity = 34/107 (31.78%), Postives = 55/107 (51.40%), Query Frame = -1 Query: 120 TFSNLIAA-----TDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEILISHNEDL 425 TF +AA +D + +K FWK + I+ AIKNI SW E+K S + VW+ + + ++ V ++ E+A+E EV E + E+L SH++ L Sbjct: 350 TFHKALAAMDSDVSDGSGQSKLKTFWKGFTILDAIKNIRDSWEEVKLSTLTGVWKKLIPTLIDDYEGFKTSVEEVSADVVEIAKELELEVEPED-VTELLQSHDKTL 455
BLAST of SMED30035747 vs. TrEMBL
Match: A0A1I8NSL8 (Uncharacterized protein OS=Stomoxys calcitrans OX=35570 GN=106088441 PE=4 SV=1) HSP 1 Score: 132.494 bits (332), Expect = 1.691e-34 Identity = 69/146 (47.26%), Postives = 100/146 (68.49%), Query Frame = -1 Query: 3 RTFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEILIS----HNEDLIFINV*RSLVDDIDTSDIVPEEKQLTARCISKPMQLISQ 428 R FS L+ ATD ++ P+MK FWKNY+I MA++NI SW E+K+SCMN WR +W EC+ K+ E+++ +P++ +EL+E AR ANFE +EE I+E+L+ NE LI + +L D T ++ PE K+LT IS+ MQLI+Q Sbjct: 179 RNFSQLLKATDGDNMPSMKDFWKNYSIRMAVENIIQSWKEVKESCMNGAWRQLWPECIIKDMEEINDVPSICKELAETARNANFEGVEEEDISELLLYDKELDNESLISAEMNNTL--DTHTLEVEPEIKKLTTAKISEAMQLITQ 322
BLAST of SMED30035747 vs. TrEMBL
Match: A0A4C1T435 (Tigger transposable element-derived protein 1 OS=Eumeta variegata OX=151549 GN=TIGD1 PE=4 SV=1) HSP 1 Score: 125.561 bits (314), Expect = 2.312e-32 Identity = 73/150 (48.67%), Postives = 95/150 (63.33%), Query Frame = -1 Query: 3 RTFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQ---VDAIPAVRRELSELAREANFEVMEEAYINEILISH-----NEDLIFINV*RSLVDDIDTSDIVPEEKQLTARCISKPMQLISQ 428 RTF L+ +TD+ D+ +MK FWKNYNIM AI+NI+LSW E+K S M WR +W E V N E+ +D +P V EL+ LAREA MEE INE+L+SH NE+L+ I++ +D S+ EEK+LT ISK MQLIS Sbjct: 103 RTFRQLLDSTDKNDQLSMKDFWKNYNIMKAIENIHLSWEEVKASSMKGSWRQLWPELV--NGEEDVIIDDVPNVSNELANLAREAGSNGMEEGDINELLLSHDKELDNENLLAIDIQHVAEEDAIISNETLEEKKLTIANISKAMQLISH 250
BLAST of SMED30035747 vs. TrEMBL
Match: M7BT63 (HTH CENPB-type domain-containing protein (Fragment) OS=Chelonia mydas OX=8469 GN=UY3_03783 PE=4 SV=1) HSP 1 Score: 107.842 bits (268), Expect = 1.133e-24 Identity = 47/103 (45.63%), Postives = 70/103 (67.96%), Query Frame = -1 Query: 120 RTFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEILISHNEDL 428 RTF LI TD E KPT++QFW++YNI+ I NI SW E+ ++CMN VW +W ECV D +PA+++++ LA++A F+ +EEA + ++L SH E+L Sbjct: 339 RTFDQLIRGTDGEGKPTIRQFWRDYNILKCINNIGESWAEVTQACMNGVWWKLWPECVNDLKGFKDVVPAMKKDILGLAKKAGFDKVEEADVTQLLQSHGEEL 441
BLAST of SMED30035747 vs. TrEMBL
Match: M7BM43 (HTH CENPB-type domain-containing protein (Fragment) OS=Chelonia mydas OX=8469 GN=UY3_03784 PE=4 SV=1) HSP 1 Score: 107.842 bits (268), Expect = 1.133e-24 Identity = 47/103 (45.63%), Postives = 70/103 (67.96%), Query Frame = -1 Query: 120 RTFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEILISHNEDL 428 RTF LI TD E KPT++QFW++YNI+ I NI SW E+ ++CMN VW +W ECV D +PA+++++ LA++A F+ +EEA + ++L SH E+L Sbjct: 339 RTFDQLIRGTDGEGKPTIRQFWRDYNILKCINNIGESWAEVTQACMNGVWWKLWPECVNDLKGFKDVVPAMKKDILGLAKKAGFDKVEEADVTQLLQSHGEEL 441
BLAST of SMED30035747 vs. TrEMBL
Match: A0A401T1F0 (HTH CENPB-type domain-containing protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0014986 PE=4 SV=1) HSP 1 Score: 106.686 bits (265), Expect = 7.304e-24 Identity = 46/103 (44.66%), Postives = 66/103 (64.08%), Query Frame = -1 Query: 120 RTFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEILISHNEDL 428 RTFS LI TD E+ P++++FW+ YNI+ I NI SW E+ SC+N VWR +W ECV D +P ++RE+ LAR F+ ++E + E+L SH E+L Sbjct: 682 RTFSQLIYETDGENAPSVREFWRGYNILEGINNIADSWNEVTSSCLNSVWRKLWPECVSDKGGSPDTVPEIQREIIALARAVGFDEVDETDVVELLESHGEEL 784
BLAST of SMED30035747 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000018.1 (pep scaffold:Pmarinus_7.0:GL482463:8529:10142:1 gene:ENSPMAG00000000016.1 transcript:ENSPMAT00000000018.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 90.1225 bits (222), Expect = 1.372e-22 Identity = 38/95 (40.00%), Postives = 55/95 (57.89%), Query Frame = -1 Query: 144 RTFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEI 428 R L A TD E KPT +FW+ Y+I A+ N+ +W E+ + MN WR IW ECV +T +P +RRE+ LAR A F+ ++E + E+ Sbjct: 271 RLVGQLAAETDGEGKPTFGEFWRGYDIRKAVDNVGAAWREVTPAHMNGAWRQIWPECVDGSTGFAKTVPEIRREIVGLARRAGFDGVDEGDVQEL 365
BLAST of SMED30035747 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006798.1 (pep scaffold:Pmarinus_7.0:GL478691:31611:33359:1 gene:ENSPMAG00000006139.1 transcript:ENSPMAT00000006798.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 74.7146 bits (182), Expect = 6.680e-17 Identity = 44/109 (40.37%), Postives = 61/109 (55.96%), Query Frame = -1 Query: 120 RTFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVR---RELSELARE---ANFEVMEEAYINEILISHNEDL 428 RT S L+ TD DKPT++ WK YNI AI NI SW ++K+S MN W IW ECV ++ P + +++ +L RE F M E + E+L S+NE+L Sbjct: 285 RTISQLLEGTDGPDKPTVRDCWKAYNIRDAINNIKSSWDDVKQSTMNCAWNAIWKECVI----YLNCFPEMSTAVQDVVDLGRELGGEGFSDMREDDVLELLDSYNEEL 389
BLAST of SMED30035747 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003106.1 (pep scaffold:Pmarinus_7.0:GL476998:37517:41189:1 gene:ENSPMAG00000002845.1 transcript:ENSPMAT00000003106.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 71.2478 bits (173), Expect = 1.196e-15 Identity = 43/109 (39.45%), Postives = 64/109 (58.72%), Query Frame = -1 Query: 117 RTFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEILISH-----NEDLI 428 +TF+ I+ATD+E PT+ +FWK ++I+ A+KNI SW E+K+S +N VWR + E V D + V + E+A + EV E + E+L SH NEDL+ Sbjct: 289 QTFAQAISATDKEGGPTLTEFWKGFSILDAVKNIAASWKEMKQSNLNGVWRKLCPEFVSNFQGFTDTVEEVTENVVEMAGQLQLEVAPED-VGELLASHAHELSNEDLL 396
BLAST of SMED30035747 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005656.1 (pep scaffold:Pmarinus_7.0:GL476578:539135:542172:1 gene:ENSPMAG00000005132.1 transcript:ENSPMAT00000005656.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.4698 bits (158), Expect = 1.194e-13 Identity = 38/101 (37.62%), Postives = 59/101 (58.42%), Query Frame = -1 Query: 123 TFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEILISHNED 425 T ++A TD+ D T+++FW++YNIM AI NIN +W E+ ++ +N W +W EC T D + + R + ELAR A F +E+ + L+S ED Sbjct: 322 TMEQIVANTDK-DVQTIREFWRSYNIMDAIDNINEAWNEVAQTTLNACWGKLWKECKHDLTGLQDPVNDIHR-IVELARNAGFHEVEQ---RKGLVSVEED 417
BLAST of SMED30035747 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010132.1 (pep scaffold:Pmarinus_7.0:GL477949:48477:51271:1 gene:ENSPMAG00000009170.1 transcript:ENSPMAT00000010132.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.151 bits (139), Expect = 4.244e-11 Identity = 29/77 (37.66%), Postives = 47/77 (61.04%), Query Frame = -1 Query: 183 LIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREA 413 ++A TD+ D +++FW +YNIM AI NIN +W E+ ++ +N W +W EC C T + + + R + ELAR+ Sbjct: 317 IVANTDK-DVQIIREFWHSYNIMDAIDNINEAWNEVAQTTLNAYWGKLWKECKCDLTGLQEPVNDIHR-IVELARKG 391
BLAST of SMED30035747 vs. Planmine SMEST
Match: SMESG000081408.1 (SMESG000081408.1) HSP 1 Score: 47.3654 bits (111), Expect = 4.505e-7 Identity = 26/75 (34.67%), Postives = 45/75 (60.00%), Query Frame = -1 Query: 117 AIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEILISHNEDLI 341 A++NI+ S E+K + MN VW+ WSEC+ N +V + +R+ + LA FE +E ++ L++ +E+LI Sbjct: 4 AVENISESLKELKSTKMNHVWKKYWSECIKTNVFEVHSPFTIRQTILHLANNFGFEDLEGDVLD--LLNSDEELI 76
BLAST of SMED30035747 vs. Planmine SMEST
Match: SMESG000032104.1 (SMESG000032104.1) HSP 1 Score: 47.7506 bits (112), Expect = 4.782e-7 Identity = 26/95 (27.37%), Postives = 48/95 (50.53%), Query Frame = -1 Query: 144 RTFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWRVIWSECVCKNTEQVDAIPAVRRELSELAREANFEVMEEAYINEI 428 +TF ++ D + T+ Q WKN+ I+ +K + L+ E++ S +N W+ IW E V N ++ + R + +LA E E+ I ++ Sbjct: 126 QTFKKILDEMDSDSSLTVSQVWKNFTILDCVKIVALAVEELRPSTLNGSWKPIWPEVVLLNN-RIQPVVDEYRAIVDLAHSLGGEGFEDLAIQDV 219 The following BLAST results are available for this feature:
BLAST of SMED30035747 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 1
BLAST of SMED30035747 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30035747 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30035747 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30035747 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30035747 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30035747 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of SMED30035747 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30035747 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30035747 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of SMED30035747 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30035747 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30035747 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30035747 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035747 ID=SMED30035747|Name=SMED30035747|organism=Schmidtea mediterranea sexual|type=transcript|length=428bpback to top protein sequence of SMED30035747-orf-1 >SMED30035747-orf-1 ID=SMED30035747-orf-1|Name=SMED30035747-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=109bp RTFSNLIAATDREDKPTMKQFWKNYNIMMAIKNINLSWLEIKKSCMNRVWback to top |