slc18a-8

Overview
Nameslc18a-8 (AKN21543.1)
Smed IDSMED30035690
Length (bp)1474
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of slc18a-8 (SMED30035690) t-SNE clustered cells

Violin plots show distribution of expression levels for slc18a-8 (SMED30035690) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of slc18a-8 (SMED30035690) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for slc18a-8 (SMED30035690) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035690

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30035690h1SMcG0010030 Contig43534newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30035690h1SMcG0010030 Contig43534uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30035690h1SMcG0010030 dd_Smed_v4_19750_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30035690h1SMcG0010030 dd_Smed_v4_19750_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of slc18a-8 vs. Ensembl Human
Match: SLC18A1 (solute carrier family 18 member A1 [Source:HGNC Symbol;Acc:HGNC:10934])

HSP 1 Score: 64.3142 bits (155), Expect = 2.291e-10
Identity = 46/197 (23.35%), Postives = 98/197 (49.75%), Query Frame = 2 
Query:  851 IYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            ++  ++DP++++  G +   N+ +  L PTLP WM    CS + + G+ +    + Y++G     ++       R+   ++G L++G  ++ +P + +   +  P  G+ + +G VD  ++P+  +L   RH S    V ++   +  +   I     G IV   GF +L +  G+IN++++P+  Y R    + +K
Sbjct:  293 LFMLLKDPYILVAAGSICFANMGVAILEPTLPIWMMQTMCSPKWQLGLAFLPASVSYLIGTNLFGVLANK--MGRWLCSLIGMLVVGTSLLCVPLAHNIFGLIGPNAGLGLAIGMVDSSMMPIMGHLVDLRHTSVYGSVYAIADVAFCMGFAIGPSTGGAIVKAIGFPWLMVITGVINIVYAPLCYYLRSPPAKEEK 487
[Close]
BLAST of slc18a-8 vs. Ensembl Human
Match: SLC18A1 (solute carrier family 18 member A1 [Source:HGNC Symbol;Acc:HGNC:10934])

HSP 1 Score: 64.3142 bits (155), Expect = 2.291e-10
Identity = 46/197 (23.35%), Postives = 98/197 (49.75%), Query Frame = 2 
Query:  851 IYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            ++  ++DP++++  G +   N+ +  L PTLP WM    CS + + G+ +    + Y++G     ++       R+   ++G L++G  ++ +P + +   +  P  G+ + +G VD  ++P+  +L   RH S    V ++   +  +   I     G IV   GF +L +  G+IN++++P+  Y R    + +K
Sbjct:  293 LFMLLKDPYILVAAGSICFANMGVAILEPTLPIWMMQTMCSPKWQLGLAFLPASVSYLIGTNLFGVLANK--MGRWLCSLIGMLVVGTSLLCVPLAHNIFGLIGPNAGLGLAIGMVDSSMMPIMGHLVDLRHTSVYGSVYAIADVAFCMGFAIGPSTGGAIVKAIGFPWLMVITGVINIVYAPLCYYLRSPPAKEEK 487
[Close]
BLAST of slc18a-8 vs. Ensembl Human
Match: SLC18A2 (solute carrier family 18 member A2 [Source:HGNC Symbol;Acc:HGNC:10935])

HSP 1 Score: 62.7734 bits (151), Expect = 7.037e-10
Identity = 51/211 (24.17%), Postives = 103/211 (48.82%), Query Frame = 2 
Query:  812 VQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRF-RYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            VQ   +K   L  + K   DP++++  G +   N+ +  L P LP WM    CS + + G+ +    + Y++G     I+   A++  R+   ++G +++G+ ++ +PF+++   +  P  G+   +G VD  ++P+   L   RH S    V ++   +  +   I     G I    GF +L   +GII+++F+P+  + R    + +K
Sbjct:  275 VQPESQKGTPLTTLLK---DPYILIAAGSICFANMGIAMLEPALPIWMMETMCSRKWQLGVAFLPASISYLIGTNIFGIL---AHKMGRWLCALLGMIIVGVSILCIPFAKNIYGLIAPNFGVGFAIGMVDSSMMPIMGYLVDLRHVSVYGSVYAIADVAFCMGYAIGPSAGGAIAKAIGFPWLMTIIGIIDILFAPLCFFLRSPPAKEEK 479
[Close]
BLAST of slc18a-8 vs. Ensembl Human
Match: SLC18A3 (solute carrier family 18 member A3 [Source:HGNC Symbol;Acc:HGNC:10936])

HSP 1 Score: 60.077 bits (144), Expect = 5.762e-9
Identity = 49/201 (24.38%), Postives = 94/201 (46.77%), Query Frame = 2 
Query:  782 ILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIIN 1384
            +L  AK       +R        I++ + DP++ +  G L   N+ +  L PT+ +WM     +SE E G+ W   F+ +++GV   + +       ++    +G  +IG    I+P  R +  + + + G+  G+  VD  ++P    L   RH S    V ++   S +++  +  ++ GHIV   GF  L++ +G+ N
Sbjct:  259 LLAVAKPFSAAARARANLPVGTPIHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMAWLPAFVPHVLGVYLTVRLAARYPHLQWLYGALGLAVIGASSCIVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQLSLGMGLAN 459
[Close]
BLAST of slc18a-8 vs. Ensembl Celegans
Match: unc-17 (Vesicular acetylcholine transporter unc-17 [Source:UniProtKB/Swiss-Prot;Acc:P34711])

HSP 1 Score: 49.6766 bits (117), Expect = 3.922e-6
Identity = 45/192 (23.44%), Postives = 94/192 (48.96%), Query Frame = 2 
Query:  851 IYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSE-IEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL 1423
            +++   DPF+  C G L + N+S+  L PT+ +WM+     +     G++W   F  +++GV   + + +      + + +VG  M GI    +P++     + +P+  +  G+  +D  ++P+  +L   RH S    V ++   S +++     ++ G IV  +GF  L I +   N+ ++P+    RK+
Sbjct:  260 MWRLFMDPFIACCSGALIMANVSLAFLEPTITTWMSEMMPDTPGWLVGVIWLPPFFPHVLGVYVTVKMLRAFPHHTWAIAMVGLAMEGIACFAIPYTTSVMQLVIPLSFVCFGIALIDTSLLPMLGHLVDTRHVSVYGSVYAIADISYSLAYAFGPIIAGWIVTNWGFTALNIIIFATNVTYAPVLFLLRKV 451
[Close]
BLAST of slc18a-8 vs. Ensembl Fly
Match: VAChT (gene:FBgn0270928 transcript:FBtr0083671)

HSP 1 Score: 62.7734 bits (151), Expect = 3.853e-10
Identity = 53/216 (24.54%), Postives = 107/216 (49.54%), Query Frame = 2 
Query:  812 VQNSRKKTKTLN----IIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL-DFQPDKN 1444
            V+ + K++K +      I++ + DP++ +C G L + N+++  L PT+  WM     +   + G+VW   F  +++GVV  + + +   + ++ +   G  + G    I+PF   +KM+ LPI  +  G+  +D  ++P    L   R+ S    + ++   S +I+  +  ++ G +V   GF  L   +   NL + P+    R + DF+P +N
Sbjct:  239 VKEAMKQSKDVQDQVIPIWRLLMDPYIAVCAGALTMSNVALAFLEPTISLWMEDNMTTDNWKIGMVWLPAFFPHVLGVVITVKMARKYPQHQWLMAAGGLALEGFSCFIIPFCSGYKMLMLPICVICFGIALIDTALLPTLGYLVDVRYVSVYGSIYAIADISYSIAYAVGPIIAGGVVEAIGFTALNFLIAFSNLAYVPVLRKLRNIYDFKPFEN 454
[Close]
BLAST of slc18a-8 vs. Ensembl Fly
Match: Vmat (gene:FBgn0260964 transcript:FBtr0091492)

HSP 1 Score: 51.9878 bits (123), Expect = 1.075e-6
Identity = 72/359 (20.06%), Postives = 147/359 (40.95%), Query Frame = 2 
Query:  380 SFIYAYTDTLLLYIIGRVLDSF-ITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHS--IGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDKNV 1447
            + I+A+  + L+  + R L     +C  + G+  L  +     + ++R  A+   +  ++    IGP +G  + E  GKS  FLIL  L +   LL +F+   +  ++         + + P+L+SL                                     I DP++++  G +   N+ +  L P+LP WM     ++  EQG+ +    + Y++G      +       R++   +G ++IG  +I +P +     + +P  G+   +G VD  ++P    L   RH +    V +L   +  +   +   L G +V   GF ++   + I+  +++P+    +      +K V
Sbjct:  281 TIIFAFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRF---TDDKERGNAMGIALGGLALGVLIGPPFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKA---------ETEPPSLKSL-------------------------------------ISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIG--RWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPLLTLLKNPPTSDEKKV 588
[Close]
BLAST of slc18a-8 vs. Ensembl Fly
Match: Vmat (gene:FBgn0260964 transcript:FBtr0310062)

HSP 1 Score: 50.8322 bits (120), Expect = 1.975e-6
Identity = 70/344 (20.35%), Postives = 143/344 (41.57%), Query Frame = 2 
Query:  380 SFIYAYTDTLLLYIIGRVLDSF-ITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHS--IGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPI 1402
            + I+A+  + L+  + R L     +C  + G+  L  +     + ++R  A+   +  ++    IGP +G  + E  GKS  FLIL  L +   LL +F+   +  ++         + + P+L+SL                                     I DP++++  G +   N+ +  L P+LP WM     ++  EQG+ +    + Y++G      +       R++   +G ++IG  +I +P +     + +P  G+   +G VD  ++P    L   RH +    V +L   +  +   +   L G +V   GF ++   + I+  +++P+
Sbjct:  281 TIIFAFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRF---TDDKERGNAMGIALGGLALGVLIGPPFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKA---------ETEPPSLKSL-------------------------------------ISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKI--GRWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPL 573
[Close]
BLAST of slc18a-8 vs. Ensembl Fly
Match: Vmat (gene:FBgn0260964 transcript:FBtr0091491)

HSP 1 Score: 50.8322 bits (120), Expect = 1.975e-6
Identity = 70/344 (20.35%), Postives = 143/344 (41.57%), Query Frame = 2 
Query:  380 SFIYAYTDTLLLYIIGRVLDSF-ITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHS--IGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPI 1402
            + I+A+  + L+  + R L     +C  + G+  L  +     + ++R  A+   +  ++    IGP +G  + E  GKS  FLIL  L +   LL +F+   +  ++         + + P+L+SL                                     I DP++++  G +   N+ +  L P+LP WM     ++  EQG+ +    + Y++G      +       R++   +G ++IG  +I +P +     + +P  G+   +G VD  ++P    L   RH +    V +L   +  +   +   L G +V   GF ++   + I+  +++P+
Sbjct:  281 TIIFAFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRF---TDDKERGNAMGIALGGLALGVLIGPPFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKA---------ETEPPSLKSL-------------------------------------ISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKI--GRWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPL 573
[Close]
BLAST of slc18a-8 vs. Ensembl Fly
Match: Vmat (gene:FBgn0260964 transcript:FBtr0091493)

HSP 1 Score: 50.8322 bits (120), Expect = 1.975e-6
Identity = 70/344 (20.35%), Postives = 143/344 (41.57%), Query Frame = 2 
Query:  380 SFIYAYTDTLLLYIIGRVLDSF-ITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHS--IGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPI 1402
            + I+A+  + L+  + R L     +C  + G+  L  +     + ++R  A+   +  ++    IGP +G  + E  GKS  FLIL  L +   LL +F+   +  ++         + + P+L+SL                                     I DP++++  G +   N+ +  L P+LP WM     ++  EQG+ +    + Y++G      +       R++   +G ++IG  +I +P +     + +P  G+   +G VD  ++P    L   RH +    V +L   +  +   +   L G +V   GF ++   + I+  +++P+
Sbjct:  281 TIIFAFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRF---TDDKERGNAMGIALGGLALGVLIGPPFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKA---------ETEPPSLKSL-------------------------------------ISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKI--GRWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPL 573
[Close]
BLAST of slc18a-8 vs. Ensembl Zebrafish
Match: slc18a3b (solute carrier family 18 member 3b [Source:NCBI gene;Acc:394082])

HSP 1 Score: 69.3218 bits (168), Expect = 3.198e-12
Identity = 50/203 (24.63%), Postives = 102/203 (50.25%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYR 1417
            F   +R+       I++ + DP++ +  G L V N+ +  L PT+ +WM S   +S+ + G+VW   FL +++GV   + +       +++   +G ++IG     +P  + +  +  P+ G+  G+  VD  ++P    L   RH S    V ++   S +++  +  ++ G IV   GF  L + +G++N++++P  L  R
Sbjct:  165 FSDRTRENMPVGTPIHRLMVDPYIAVVAGALTVCNIPLAFLEPTIANWMESTMDASKWQMGLVWLPAFLPHVLGVYITVRLAARYPERQWFYGALGMVIIGASSCTVPACKTFGELVFPLCGICFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYAMGPVVAGQIVHNLGFVQLNLGMGLVNVLYAPALLLLR 367
[Close]
BLAST of slc18a-8 vs. Ensembl Zebrafish
Match: slc18a3a (solute carrier family 18 member 3a [Source:NCBI gene;Acc:559347])

HSP 1 Score: 68.5514 bits (166), Expect = 7.244e-12
Identity = 51/207 (24.64%), Postives = 101/207 (48.79%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDF 1429
            F   +R+       IYK + DP++ +  G L   N+ +  L PT+ +WM     +S+ + GI W   F  +I+GV   + +       +++   +G ++IG    I+P  ++++ + +P+ G+  G+  VD  ++P    L   RH S    V ++   S  ++  +  ++ G IV   GF  L + +G+ N++++P  L  R +  
Sbjct:  239 FSSRTRENMPVGTPIYKLMIDPYIAVVAGALTTCNIPLAFLEPTIANWMEETMNASQWQIGITWLPAFFPHILGVYLTVKLAAKYPHLQWFYGALGMVIIGASSCIVPACKNFEQLIIPLCGVCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYCVAYALGPIVAGKIVHDLGFVQLNLGMGLANVLYAPALLLLRNVSL 445
[Close]
BLAST of slc18a-8 vs. Ensembl Zebrafish
Match: slc18a2 (solute carrier family 18 member 2 [Source:ZFIN;Acc:ZDB-GENE-080514-1])

HSP 1 Score: 62.7734 bits (151), Expect = 4.102e-10
Identity = 49/229 (21.40%), Postives = 112/229 (48.91%), Query Frame = 2 
Query:  770 ANVQILDKA-KMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRF-RYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDKNVN 1450
            A + +LD   +++I    S+  ++    +   ++DP++++  G +   N+++  L P LP WM    C  + + GI +    + Y++G     ++   A++  R+   ++G L++GI ++ +P ++D   + +P  G+   +G VD  ++P+   L   RH S    V ++   +  +   +     G I    GF +L   +G+++++F+P+  + R      +K  +
Sbjct:  246 AVLAVLDGGERINICSLKSQDSSQKGTSLITLMKDPYILIAAGSICFANMAIAMLEPALPIWMMETMCPRKWQLGIAFVPASISYLIGTNIFAVL---AHKMGRWLCSLIGMLLVGISILCVPLAKDIYGLIVPNFGVGFAIGMVDSSMMPIMGYLVDLRHVSVYGSVYAIADVAFCMGFALGPSAGGAIARSIGFPWLMTIIGLVDIMFAPLCFFLRNPPANEEKMAD 471
[Close]
BLAST of slc18a-8 vs. Ensembl Zebrafish
Match: slc18a2 (solute carrier family 18 member 2 [Source:ZFIN;Acc:ZDB-GENE-080514-1])

HSP 1 Score: 61.6178 bits (148), Expect = 1.102e-9
Identity = 50/227 (22.03%), Postives = 111/227 (48.90%), Query Frame = 2 
Query:  770 ANVQILDKAKMDIFVQNSR--KKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRF-RYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            A + +LD A     +Q S+   +++    +   ++DP++++  G +   N+++  L P LP WM    C  + + GI +    + Y++G     ++   A++  R+   ++G L++GI ++ +P ++D   + +P  G+   +G VD  ++P+   L   RH S    V ++   +  +   +     G I    GF +L   +G+++++F+P+  + R      +K
Sbjct:  304 AVLAVLDGALQLFVLQPSKVEPESQKGTSLITLMKDPYILIAAGSICFANMAIAMLEPALPIWMMETMCPRKWQLGIAFVPASISYLIGTNIFAVL---AHKMGRWLCSLIGMLLVGISILCVPLAKDIYGLIVPNFGVGFAIGMVDSSMMPIMGYLVDLRHVSVYGSVYAIADVAFCMGFALGPSAGGAIARSIGFPWLMTIIGLVDIMFAPLCFFLRNPPANEEK 527
[Close]
BLAST of slc18a-8 vs. Ensembl Zebrafish
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:571483])

HSP 1 Score: 56.6102 bits (135), Expect = 3.819e-8
Identity = 53/219 (24.20%), Postives = 104/219 (47.49%), Query Frame = 2 
Query:  770 ANVQILDKAKMDIFVQNSRKKTKTLNIIYKFI--RDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRK 1420
            A + + D A     +Q S+    ++     FI  +DP++++  G L   N+ +  L PTLP WM    C+ + + G+ +    + Y++G     I+       R+   +VG  ++GI ++ +PF+++   +  P  G+   VG VD  +  +   L   RH S    V ++   +L +   +     G +V   GF  L + +GIIN++++P+  + + 
Sbjct:  253 AFLAMFDGALQLCILQPSKISPTSMEGTPLFILLKDPYILISAGSLCFANMGVAVLEPTLPIWMMQTMCAPKWQLGVAFLPASVSYLIGTNLFGILANK--MGRWLCSMVGMFIVGISLLCVPFAKNLYGLIGPNGGLGFAVGMVDTSMFAIMGYLVDIRHVSVYGSVYAIADVALCVGFAVGPSTGGALVRTIGFPNLMVIIGIINILYAPLCFFLKN 469
[Close]
BLAST of slc18a-8 vs. Ensembl Xenopus
Match: gtf2e1.2 (general transcription factor IIE, polypeptide 1, alpha 56kDa, gene 2 [Source:Xenbase;Acc:XB-GENE-6001963])

HSP 1 Score: 68.1662 bits (165), Expect = 1.345e-11
Identity = 48/196 (24.49%), Postives = 100/196 (51.02%), Query Frame = 2 
Query:  854 YKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            ++ +RDP++++    L++ N+++  L PTLP WM    CS + + GI +    + Y++      I+       R+   ++G +++G  ++ +P +R+   +  P   + + +G VD  ++P+   L   RH S    + ++   +L +   +   L G I    GF +L + +GIIN++FSP+ +  R    + +K
Sbjct:  261 HELLRDPYILVAAACLWIANMAVGMLEPTLPIWMMETMCSPDWQLGIAFLPASVAYLICTNLFGILSHK--MGRWLCSMLGMIVVGGGLLCVPLARNIYGLIGPNAAIGIALGMVDSSIMPIMGYLVDLRHTSVYGGIYAICDTALCLGFAVGPSLAGVIASNIGFSWLMVIIGIINILFSPLCILLRNPPAKEEK 454
[Close]
BLAST of slc18a-8 vs. Ensembl Xenopus
Match: gtf2e1.2 (general transcription factor IIE, polypeptide 1, alpha 56kDa, gene 2 [Source:Xenbase;Acc:XB-GENE-6001963])

HSP 1 Score: 67.781 bits (164), Expect = 1.437e-11
Identity = 49/199 (24.62%), Postives = 102/199 (51.26%), Query Frame = 2 
Query:  854 YKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDKNVN 1450
            ++ +RDP++++    L++ N+++  L PTLP WM    CS + + GI +    + Y++      I+       R+   ++G +++G  ++ +P +R+   +  P   + + +G VD  ++P+   L   RH S    + ++   +L +   +   L G I    GF +L + +GIIN++FSP+ +  R    + +K V+
Sbjct:  245 HELLRDPYILVAAACLWIANMAVGMLEPTLPIWMMETMCSPDWQLGIAFLPASVAYLICTNLFGILSHK--MGRWLCSMLGMIVVGGGLLCVPLARNIYGLIGPNAAIGIALGMVDSSIMPIMGYLVDLRHTSVYGGIYAICDTALCLGFAVGPSLAGVIASNIGFSWLMVIIGIINILFSPLCILLRNPPAKEEKVVS 441
[Close]
BLAST of slc18a-8 vs. Ensembl Xenopus
Match: slc18a3 (solute carrier family 18 (vesicular acetylcholine transporter), member 3 [Source:NCBI gene;Acc:780320])

HSP 1 Score: 60.8474 bits (146), Expect = 2.455e-9
Identity = 46/205 (22.44%), Postives = 101/205 (49.27%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL 1423
            F   +R        I++ + DP++ +  G L   N+ +  L PT+ +WM +   +SE + G+ W   F  +++GV   + +  +   ++++   +G ++IG     +P  +++  + +P+ G+  G+  VD  ++P    L   RH S    V ++   S  ++  +  ++   IV   GF  L + +G+ N++++P  L+ R +
Sbjct:  249 FTNRTRANMPIGTPIHRLMIDPYIAVVAGALTTCNIPLAFLEPTIANWMKTTMDASEWQMGLTWLPAFFPHVLGVYITVKLAANYPHYQWFYGAIGMVIIGASSCTVPACKNFWELIVPLCGICFGIALVDTALLPTLALLVDVRHVSVYGSVYAIADISYCVAYALGPVVASQIVHTTGFTQLNLGMGLANVLYAPALLFLRNV 453
[Close]
BLAST of slc18a-8 vs. Ensembl Xenopus
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:Xenbase;Acc:XB-GENE-6054064])

HSP 1 Score: 56.6102 bits (135), Expect = 4.531e-8
Identity = 44/198 (22.22%), Postives = 96/198 (48.48%), Query Frame = 2 
Query:  857 KFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDKNVN 1450
              ++DP+++L  G + + N+ +    PTLP WM    C+ +   GIV+    + Y+V    +  + +     R+   ++G ++ GI  + +P + ++  +  PI    + +G VD  ++PL   L   RH S    V +++  +L +   +   + G      G  +L + + ++N+++SP+ +  R    + +K V+
Sbjct:  201 ALLKDPYIVLAAGSICLTNMVIAMAEPTLPVWMLGTMCTPDWLLGIVFFPASISYLVCTNLLPRLSQKIG--RWLCSLLGMVLAGISFLCVPLAVNFYGLITPIAAFGISIGMVDVSMVPLMAYLVDLRHSSVYGGVYAISDIALNLGFAVGPCVAGITAKAIGLPWLMVIIAVLNIMYSPLCILLRNPPGKSEKMVS 396
[Close]
BLAST of slc18a-8 vs. Ensembl Xenopus
Match: gtf2e1.2 (general transcription factor IIE, polypeptide 1, alpha 56kDa, gene 2 [Source:Xenbase;Acc:XB-GENE-6001963])

HSP 1 Score: 56.6102 bits (135), Expect = 4.904e-8
Identity = 45/181 (24.86%), Postives = 90/181 (49.72%), Query Frame = 2 
Query:  899 LFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            L++ N+++  L PTLP WM    CS + + GI +    + Y++      I+       R+   ++G +++G  ++ +P +R+   +  P   + + +G VD  ++P+   L   RH S    + ++   +L +   +   L G I    GF +L + +GIIN++FSP+ +  R    + +K
Sbjct:  245 LWIANMAVGMLEPTLPIWMMETMCSPDWQLGIAFLPASVAYLICTNLFGILSHK--MGRWLCSMLGMIVVGGGLLCVPLARNIYGLIGPNAAIGIALGMVDSSIMPIMGYLVDLRHTSVYGGIYAICDTALCLGFAVGPSLAGVIASNIGFSWLMVIIGIINILFSPLCILLRNPPAKEEK 423
[Close]
BLAST of slc18a-8 vs. Ensembl Mouse
Match: Slc18a2 (solute carrier family 18 (vesicular monoamine), member 2 [Source:MGI Symbol;Acc:MGI:106677])

HSP 1 Score: 62.7734 bits (151), Expect = 4.941e-10
Identity = 51/211 (24.17%), Postives = 104/211 (49.29%), Query Frame = 2 
Query:  812 VQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRF-RYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            VQ   +K   L  +   ++DP++++  G +   N+ +  L P LP WM    CS + + G+ +    + Y++G     I+   A++  R+   ++G +++GI ++ +PF+++   +  P  G+   +G VD  ++P+   L   RH S    V ++   +  +   I     G I    GF +L   +GII+++F+P+  + R    + +K
Sbjct:  278 VQPESQKGTPLTTL---LKDPYILIAAGSICFANMGIAMLEPALPIWMMETMCSRKWQLGVAFLPASISYLIGTNIFGIL---AHKMGRWLCALLGMIVVGISILCIPFAKNIYGLIAPNFGVGFAIGMVDSSMMPIMGYLVDLRHVSVYGSVYAIADVAFCMGYAIGPSAGGAIAKAIGFPWLMTIIGIIDIVFAPLCFFLRSPPAKEEK 482
[Close]
BLAST of slc18a-8 vs. Ensembl Mouse
Match: Slc18a3 (solute carrier family 18 (vesicular monoamine), member 3 [Source:MGI Symbol;Acc:MGI:1101061])

HSP 1 Score: 61.2326 bits (147), Expect = 1.468e-9
Identity = 49/201 (24.38%), Postives = 96/201 (47.76%), Query Frame = 2 
Query:  782 ILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIIN 1384
            +L  AK       +R        I++ + DP++ +  G L   N+ +  L PT+ +WM     +SE E G+VW   F+ +++GV   + +       ++    +G  +IG+   ++P  R +  + + + G+  G+  VD  ++P    L   RH S    V ++   S +++  +  ++ GHIV   GF  L++ +G+ N
Sbjct:  259 LLAVAKPFSAAARARANLPVGTPIHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMVWLPAFVPHVLGVYLTVRLAARYPHLQWLYGALGLAVIGVSSCVVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQLSLGMGLAN 459
[Close]
BLAST of slc18a-8 vs. Ensembl Mouse
Match: Slc18a1 (solute carrier family 18 (vesicular monoamine), member 1 [Source:MGI Symbol;Acc:MGI:106684])

HSP 1 Score: 60.077 bits (144), Expect = 4.141e-9
Identity = 46/190 (24.21%), Postives = 94/190 (49.47%), Query Frame = 2 
Query:  851 IYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRK 1420
            +   ++DP++++  G + + N+ +  L PTLP WM    CS E + G+ +    + Y++G     ++       R+   +VG + +GI ++ +P + +   +  P  G+   +G VD  ++P+   L   RH S    V ++   +  +   I     G IV + GF +L + +G IN+I++P+  + + 
Sbjct:  290 LLTLLKDPYILVAAGSICLANMGVAILEPTLPIWMMQTMCSPEWQLGLAFLPASVAYLIGTNLFGVLANK--MGRWLCSLVGMVAVGISLLCVPLAHNIFGLIGPNAGLGFAIGMVDSSLMPIMGYLVDLRHTSVYGSVYAIADVAFCVGFAIGPSTGGVIVPVIGFPWLMVIIGTINIIYAPLCCFLQN 477
[Close]
BLAST of slc18a-8 vs. UniProt/SwissProt
Match: sp|P81721|VACHT_TETCF (Vesicular acetylcholine transporter OS=Tetronarce californica OX=7787 PE=2 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 4.408e-12
Identity = 51/205 (24.88%), Postives = 103/205 (50.24%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL 1423
            F   +R+       IYK + DP++ +  G L   N+ +  L PT+ +WM     +SE + GI W   F  +I+GV   + +      ++++   VG ++IG     +P  R+++ + +P+  +  G+  VD  ++P    L   R+ S    V ++   S +++  +  ++ G IV   GF  L + +G++N++++P  L+ R +
Sbjct:  253 FASRTRENMLQGTPIYKLMIDPYIAVVAGALTTCNIPLAFLEPTISNWMKKTMNASEWQMGITWLPAFFPHILGVYITVKLAAKYPNYQWFYGAVGLVIIGASSCTIPACRNFEELIIPLCALCFGIALVDTALLPTLAFLVDIRYVSVYGSVYAIADISYSVAYALGPIMAGQIVHDLGFVQLNLGMGLVNILYAPALLFLRNV 457
[Close]
BLAST of slc18a-8 vs. UniProt/SwissProt
Match: sp|Q91498|VACHT_TORMA (Vesicular acetylcholine transporter OS=Torpedo marmorata OX=7788 PE=2 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 1.401e-11
Identity = 52/205 (25.37%), Postives = 102/205 (49.76%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL 1423
            F   +R  T     IYK + DP++ +  G L   N+ +  L PT+ +WM     +SE + GI W   F  +I+GV   + +      +++    VG ++IG     +P  R+++ + +P+  +  G+  VD  ++P    L   R+ S    V ++   S +++  +  ++ G IV   GF  L + +G++N++++P  L+ R +
Sbjct:  249 FASRTRVNTLQGTPIYKLMIDPYIAVVAGALTTCNIPLAFLEPTISNWMKKTMNASEWQMGITWLPAFFPHILGVYITVKLAAKYPNYQWLYGAVGLVIIGASSCTIPACRNFEELIIPLCALCFGIALVDTALLPTLAFLVDIRYVSVYGSVYAIADISYSVAYALGPIMAGQIVHDLGFVQLNLGMGLVNILYAPGLLFLRNV 453
[Close]
BLAST of slc18a-8 vs. UniProt/SwissProt
Match: sp|P59845|VACHB_DANRE (Probable vesicular acetylcholine transporter-B OS=Danio rerio OX=7955 GN=slc18a3b PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.929e-11
Identity = 50/203 (24.63%), Postives = 102/203 (50.25%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYR 1417
            F   +R+       I++ + DP++ +  G L V N+ +  L PT+ +WM S   +S+ + G+VW   FL +++GV   + +       +++   +G ++IG     +P  + +  +  P+ G+  G+  VD  ++P    L   RH S    V ++   S +++  +  ++ G IV   GF  L + +G++N++++P  L  R
Sbjct:  227 FSDRTRENMPVGTPIHRLMVDPYIAVVAGALTVCNIPLAFLEPTIANWMESTMDASKWQMGLVWLPAFLPHVLGVYITVRLAARYPERQWFYGALGMVIIGASSCTVPACKTFGELVFPLCGICFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYAMGPVVAGQIVHNLGFVQLNLGMGLVNVLYAPALLLLR 429
[Close]
BLAST of slc18a-8 vs. UniProt/SwissProt
Match: sp|Q08C75|VACHA_DANRE (Probable vesicular acetylcholine transporter-A OS=Danio rerio OX=7955 GN=slc18a3a PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.468e-11
Identity = 51/207 (24.64%), Postives = 101/207 (48.79%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDF 1429
            F   +R+       IYK + DP++ +  G L   N+ +  L PT+ +WM     +S+ + GI W   F  +I+GV   + +       +++   +G ++IG    I+P  ++++ + +P+ G+  G+  VD  ++P    L   RH S    V ++   S  ++  +  ++ G IV   GF  L + +G+ N++++P  L  R +  
Sbjct:  239 FSSRTRENMPVGTPIYKLMIDPYIAVVAGALTTCNIPLAFLEPTIANWMEETMNASQWQIGITWLPAFFPHILGVYLTVKLAAKYPHLQWFYGALGMVIIGASSCIVPACKNFEQLIIPLCGVCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYCVAYALGPIVAGKIVHDLGFVQLNLGMGLANVLYAPALLLLRNVSL 445
[Close]
BLAST of slc18a-8 vs. UniProt/SwissProt
Match: sp|Q91514|VACHT_TORTO (Vesicular acetylcholine transporter OS=Torpedo torpedo OX=30481 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 7.032e-11
Identity = 50/205 (24.39%), Postives = 102/205 (49.76%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL 1423
            F   +R  T     I+K + DP++ +  G L   N+ +  L PT+ +WM     +SE + GI W   F  +I+GV   + +      +++     G ++IG+    +P  R+++ + +P+  +  G+  VD  ++P    L   R+ S    V ++   S +++  +  ++ G IV   GF  L + +G++N++++P  L+ R +
Sbjct:  249 FASRTRGNTLQGTPIHKLMIDPYIAVVAGALTTCNIPLAFLEPTISNWMKKTMNASEWQMGITWLPAFFPHILGVYITVKLAAKYPNYQWLYGAFGLVIIGVSSCTIPACRNFEELIIPLCALCFGIALVDTALLPTLAFLVDIRYVSVYGSVYAIADISYSVAYALGPIMAGQIVHDLGFVQLNLGMGLVNILYAPALLFLRNV 453
[Close]
BLAST of slc18a-8 vs. TrEMBL
Match: A0A0H3YK88 (Slc18a-8 OS=Schmidtea mediterranea OX=79327 GN=slc18a-8 PE=2 SV=1)

HSP 1 Score: 807.364 bits (2084), Expect = 0.000e+0
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 2 
Query:   50 MFFKFDNLLAGVVVSLNFFLMVLQENSLYAILPKYFSNQTSISARNETVLINSTVPQNCSFQKPPYVVVSQIFLSKNVAMXXXXXXXXXXXXXXXXXXXXXXSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDKNVN 1450
            MFFKFDNLLAGVVVSLNFFLMVLQENSLYAILPKYFSNQTSISARNETVLINSTVPQNCSFQKPPYVVVSQIFLSKNVAMIFLNIFIGIIIYKLNIYIILILSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYIVGVVTVIIVDKHAYRFRYYVPVVGHLMIGILMIILPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNIVSSLTSQSLTISSVISTLLCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDKNVN
Sbjct:    1 MFFKFDNLLAGVVVSLNFFLMVLQENSLYAILPKYFSNQTSISARNETVLINSTVPQNCSFQKPPYVVVSQIFLSKNVAMIFLNIFIGIIIYKLNIYIILILSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYIVGVVTVIIVDKHAYRFRYYVPVVGHLMIGILMIILPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNIVSSLTSQSLTISSVISTLLCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDKNVN 467
[Close]
BLAST of slc18a-8 vs. TrEMBL
Match: A0A0H3YJ27 (Slc18a-1 OS=Schmidtea mediterranea OX=79327 GN=slc18a-1 PE=2 SV=1)

HSP 1 Score: 307.375 bits (786), Expect = 8.460e-96
Identity = 204/458 (44.54%), Postives = 297/458 (64.85%), Query Frame = 2 
Query:   50 MFFKFDNLLAGVVVSLNFFLMVLQENSLYAILPKYFSNQTSISARNETVLINS--TVPQNCSFQKPPYVVVSQIFLSKNVAMXXXXXXXXXXXXXXXXXXXXXXSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSE----IKLEGQRQKPNLESLDECINEANVQIL--DKAKMD---IFVQNSRKKTKTLNII----YKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGI 1378
            M FK D+ LA +V+ LNF+++ +QE+SLY ILPKY  ++   +  NET   N+  T+ Q C  QKPPY +VS IFLSK++A  F N  IG  + K+N +IIL++   +GI ++ IYA  +++ +YI+GR+LD+F++C + IG + ++SKLF  +  R R++ L N +YF+SHSIGP YGAEVD R GKS VF +L+PLWI  +  T  LY R   R  +S+     KL           LD+  NE  V I+    +KM       +  +K  K  N I    YK +++P+V  CL QLF+VNLSM++LP TLPSWM    C++EI+QG+VW FGF+GY+ GV  ++++ K AY+ R++ P++  +++GIL+I+LP S DW+++F+PI+GMY  VG ++Q++ PLT  LA     S+   ++SLTSQSLT+S V+ TL  G I   FGF  L I LGI
Sbjct:    1 MSFKCDDCLAAIVICLNFYMLGIQESSLYTILPKYLVDEPFNATNNETFKPNTSRTIFQ-CHLQKPPYFIVSMIFLSKSLAQFFFNSIIGCFVDKVNNFIILLIGQGLGIIIALIYALNNSITMYIVGRLLDAFVSCSLFIGGFSIVSKLFSNVNTRGRFFGLGNTLYFVSHSIGPVYGAEVDVRLGKSAVFFLLIPLWIFTMAATFILYRRTEKRCNKSKNNFNEKLSIGVTPDETNELDD-HNELKVAIIISSNSKMPEKYSDFKGEKKFQKENNEIFMKFYKIMKNPYVSSCLCQLFIVNLSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLIVLLLKCAYQLRFFSPIIAQIIVGILLILLPLSHDWRLLFIPIIGMYFSVGVIEQVLFPLTTYLADHHFNSDYGSMASLTSQSLTLSGVVGTLSSGPIAQRFGFRSLCICLGI 456
[Close]
BLAST of slc18a-8 vs. TrEMBL
Match: A0A3M7Q6N1 (Vesicular acetylcholine transporter OS=Brachionus plicatilis OX=10195 GN=BpHYR1_014320 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.366e-15
Identity = 83/316 (26.27%), Postives = 152/316 (48.10%), Query Frame = 2 
Query:  503 IEVRDRYYALVNIIYFISHS--IGPFYGAEVDERFGKSTVFLILVPLWIV--CLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL-DFQP 1435
            +E  +R  AL   + FIS    + P +G  + E  G +  F++L  L +V  C+LL V        R  +  + +E    KP                              K T     I+K  +DP++++C G L V N+S+  L PT+  WM +   ++E + G VW  GF+ +++GV+  I V+KH  ++++ +   G  + G   +I+PF  ++  + +PI G+  G+  VD  ++P    L   RH S    + ++   S +++  I  +L G+I+ LFGF  L + + + N++++P+  + R   +F+P
Sbjct:  182 VEENERSRALGIALAFISFGSLVAPPFGGVLYEYLGINVPFIVLAFLALVDGCMLLLVM-------RPHKMAMDMEQNEYKP------------------------------KGTP----IWKLFQDPYILVCAGALVVANISLAFLEPTIAVWMRNSMNATESQIGSVWLPGFIPHLLGVLFTIWVNKHFPQYQWLLAAGGLALEGASCLIIPFCTNFLTIMIPISGICFGIALVDTAILPTLAYLVDVRHTSVYGSIYAIADISYSLAFAIGPILAGNIIYLFGFTALNVGIFLSNVLYAPVIYFLRSFYEFKP 456
[Close]
BLAST of slc18a-8 vs. TrEMBL
Match: A0A267GQ21 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025894g2 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.063e-15
Identity = 84/369 (22.76%), Postives = 157/369 (42.55%), Query Frame = 2 
Query:  383 FIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLW--IVCLLLTVFLYWRNRNRSKRSEIKL--------------------EGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL 1423
             +Y   D+  ++++ R ++   +  I       ++  F  + +R+R   + N + F ++++GP YG  + ER G+  VFL  +P++    CLL+ +      R R  R E                        G     +  +      +A+  ++     D  V   R K   L   ++ + DP +  C G L V NLS   L  TL  WM   + +++ ++G+VW      +    V  + +   A  +R +V   G ++ G+   +LPF        LPI  ++  +  +D  ++PL  +L   RH      VS +   S +I++       G I +  GF +L + L ++ L ++PI L+  +L
Sbjct:  117 LLYGLGDSYWVFLLARAVNGAGSAAIGTSGLAQVATCFTSLRMRNRAIGIANFVLFSANALGPAYGGFIYERTGRGVVFLAYLPMFAATACLLVPLLRQGLVRQRGGRPEGPAAPQSPQHPPPSQQQQPQQQDAGDAVAVSSPTSSPAAIDADHLVVPDNDNDEEVAAKRPKPTPL---WRLLTDPHIAACAGGLIVANLSHNCLAATLGIWMHHRYEATDTQKGLVWLPTLPFHFAATVASVWLCNRAPAWRGWVAAGGLVLGGLACALLPFMPGKAWCTLPIGLVFASIAVLDVTLLPLLSHLVDRRHTPVYGSVSCIAHASFSIATAFGAFAAGPIFLHLGFAYLFVILCVVCLAYTPIVLFTSRL 482
[Close]
BLAST of slc18a-8 vs. TrEMBL
Match: A0A267HB05 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025894g1 PE=4 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 8.379e-15
Identity = 90/381 (23.62%), Postives = 161/381 (42.26%), Query Frame = 2 
Query:  362 FVGIGVSFI----YAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLW--IVCLLLTVFLYWRNRNRSKRSEIKLEGQRQK---------------------PNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL 1423
             VGI    I    Y   D+  ++++ R ++   +  I       ++  F  + +R+R   + N + F ++++GP YG  + ER G+  VFL  +P++    CLL+ +      R R  R E     Q  +                      +  S    I+  ++ + D    D  V   R K   L   ++ + DP +  C G L V NLS   L  TL  WM   + +++ ++G+VW      +    V  + +   A  +R +V   G  + G+   +LPF        LPI  ++  +  +D  ++PL  +L   RH      VS +   S +I++       G I +  GF +L + L ++ L ++PI L+  +L
Sbjct:  145 LVGIAADCISILLYGLGDSYWVFLLARAVNGAGSAAIGTSGLAQVATCFTSLRMRNRAIGIANFVLFSANALGPAYGGFIYERTGRGVVFLAYLPMFAATACLLVPLLRQGLVRQRGGRPEGPAAPQSPQHRPPSQQQQPQQQDAGDAVAVSSPTSSPAAIDADHLVVPDSGN-DEEVAAKRPKPTPL---WRLLTDPHIAACAGGLIVANLSHNCLAATLGIWMHHRYEATDTQKGLVWLPTLPFHFAATVASVWLCNRAPAWRGWVAAGGLALGGLACALLPFMPGKAWCTLPIGLVFASIAVLDVTLLPLLSHLVDRRHTPVYGSVSCIAHASFSIATAFGAFAAGPIFLHLGFAYLFVILCVVCLAYTPIVLFTSRL 521
[Close]
BLAST of slc18a-8 vs. Ensembl Cavefish
Match: slc18a3b (solute carrier family 18 member 3b [Source:NCBI gene;Acc:394082])

HSP 1 Score: 72.0182 bits (175), Expect = 4.351e-13
Identity = 50/211 (23.70%), Postives = 106/211 (50.24%), Query Frame = 2 
Query:  815 QNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL-DFQPDKN 1444
            Q +R+       +++ I DP++ +  G L V N+ +  L PT+  WM +   +++ + G+ W   F  +++GV   + + +     +++   VG L+IG    ++P  + +  + LP+ G+  G+  VD  ++P    L   R+ S    V ++   S +++  +  +  GHIV   GF  L + +G+ N++++P  L  R +   +P ++
Sbjct:  213 QGARQNQPAGVPLHRLIMDPYIAVVAGALTVCNIPLAFLEPTIAGWMENTMHATKWQIGLAWLPSFFPHVLGVYLTVKLAERYPSMQWFYGAVGMLIIGASSCVVPACKSFSQLMLPLCGICFGIALVDTALLPTMAFLVDVRYASVYGSVYAIADISYSVAYALGPVAAGHIVHTMGFAQLNLGMGLANVLYAPALLALRHVCQIKPSRS 423
[Close]
BLAST of slc18a-8 vs. Ensembl Cavefish
Match: slc18a3a (solute carrier family 18 member A3 [Source:NCBI gene;Acc:103044616])

HSP 1 Score: 68.5514 bits (166), Expect = 6.605e-12
Identity = 49/205 (23.90%), Postives = 100/205 (48.78%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL 1423
            F   +R+       IYK + DP++ +  G L   N+ +  L PT+ +WM     SS+ + G+ W   F  +++GV   + +       +++   +G ++IG     +P  ++++ + +P+ G+  G+  VD  ++P    L   RH S    V ++   S  ++  +  ++ G IV   GF  L + +G+ N++++P  L  R +
Sbjct:  245 FSSRTRENMPVGPPIYKLMIDPYIAVVAGALTTCNIPLAFLEPTIANWMEETMDSSQWQIGLTWLPAFFPHVLGVYITVKLAAQYPHLQWFYGALGMVIIGASSCTVPACKNFEQLIIPLCGICFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYCVAYAMGPIVAGKIVHDLGFVQLNLGMGLANVLYAPALLLLRNV 449
[Close]
BLAST of slc18a-8 vs. Ensembl Cavefish
Match: slc18a2 (synaptic vesicular amine transporter [Source:NCBI gene;Acc:103032219])

HSP 1 Score: 65.4698 bits (158), Expect = 5.107e-11
Identity = 51/226 (22.57%), Postives = 109/226 (48.23%), Query Frame = 2 
Query:  770 ANVQILDKAKMDIFVQNSR--KKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            A + +LD A     +Q S+   +++    +   ++DP++++  G +   N+++  L P LP WM    C+ + + G+ +    + Y++G     ++       R+   ++G L++GI ++ +PF++D   + LP  G+   +G VD  ++P+   L   RH S    V ++   +  +         G I    GF +L   +GI++++F+P+  + R      +K
Sbjct:  254 AVLAVLDGALQLFVLQPSKVEPESQKGTSLMTLMKDPYILIAAGSICFANMAIAMLEPALPIWMMETMCARKWQLGVAFLPASVSYLIGTNIFAVLANK--MGRWLCALIGMLLVGISILCVPFAKDIYGLILPNFGVGFAIGMVDSSMMPIMGYLVDLRHISVYGSVYAIADVAFCMGFAFGPSAGGAIARSIGFPWLMTIIGIVDILFAPLCFFLRSPPANEEK 477
[Close]
BLAST of slc18a-8 vs. Ensembl Cavefish
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:103028146])

HSP 1 Score: 58.151 bits (139), Expect = 1.213e-8
Identity = 46/197 (23.35%), Postives = 97/197 (49.24%), Query Frame = 2 
Query:  851 IYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            +   ++DP++++  G L   N+ +  L PTLP WM    CS + + G+ +    + Y++G     ++       R+   ++G  ++GI +I +P + +   +  P  G+   +G VD  ++ +   L   RH S    V ++   +L +   I     G IV + GF +L + +GI+N++++P+  + +    + +K
Sbjct:  289 LLTLLKDPYILISAGSLCFANMGVAILEPTLPIWMMQTMCSQKWQLGVAFLPASVSYLIGTNLFGLLANK--MGRWLCSMIGMFIVGISLICVPLASNIYGLIGPNGGLGFAIGMVDSSMMAIMGYLVDIRHSSVYGSVYAIADVALCMGFAIGPSTGGAIVRVIGFPYLMVMIGIVNILYAPLCYFLKNPAVREEK 483
[Close]
BLAST of slc18a-8 vs. Ensembl Cavefish
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:103028146])

HSP 1 Score: 57.7658 bits (138), Expect = 1.416e-8
Identity = 51/226 (22.57%), Postives = 108/226 (47.79%), Query Frame = 2 
Query:  770 ANVQILDKAKMDIFVQNSRKKTKTLN--IIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            A + + D A     +Q S+    ++    +   ++DP++++  G L   N+ +  L PTLP WM    CS + + G+ +    + Y++G     ++       R+   ++G  ++GI +I +P + +   +  P  G+   +G VD  ++ +   L   RH S    V ++   +L +   I     G IV + GF +L + +GI+N++++P+  + +    + +K
Sbjct:  252 AFLALFDGALQLCILQPSKISPGSVEGTPLLTLLKDPYILISAGSLCFANMGVAILEPTLPIWMMQTMCSQKWQLGVAFLPASVSYLIGTNLFGLLANK--MGRWLCSMIGMFIVGISLICVPLASNIYGLIGPNGGLGFAIGMVDSSMMAIMGYLVDIRHSSVYGSVYAIADVALCMGFAIGPSTGGAIVRVIGFPYLMVMIGIVNILYAPLCYFLKNPAVREEK 475
[Close]
BLAST of slc18a-8 vs. Ensembl Sea Lamprey
Match: slc18a3a (solute carrier family 18 member 3a [Source:NCBI gene;Acc:559347])

HSP 1 Score: 75.485 bits (184), Expect = 8.128e-15
Identity = 51/203 (25.12%), Postives = 106/203 (52.22%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYR 1417
            F   SR+       IY+ + DP++++  G L + N+ +  L PT+ SWM     ++E ++G+ W   F  +++GV   + +     + +++   +G ++IG    ++P  R ++ +  P+ G+  G+  VD  ++PL   L   RH S    V ++   S +++  +  ++ G IV   GF  L++ +G+ N++++P+ L  R
Sbjct:  259 FSSRSRENMPQGTPIYRLMMDPYIVVVAGALTMANIPLAFLEPTIASWMKKTMGANEWQRGMTWLPAFFPHVLGVYITVKLAAKYPQLQWFYGGLGLVIIGSSSCLVPACRTFEELIAPLCGICFGIALVDTALLPLLAFLVDVRHASVYGSVYAIADISYSLAYAMGPIVAGQIVHTMGFVQLSLGMGLTNVLYAPMLLLLR 461
[Close]
BLAST of slc18a-8 vs. Ensembl Sea Lamprey
Match: slc18a2 (solute carrier family 18 member 2 [Source:ZFIN;Acc:ZDB-GENE-080514-1])

HSP 1 Score: 66.6254 bits (161), Expect = 5.040e-12
Identity = 53/227 (23.35%), Postives = 110/227 (48.46%), Query Frame = 2 
Query:  770 ANVQILDKAKMDIFVQNSRKK--TKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRF-RYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            A + + D A   + +Q S+ +  ++T   +   +RDP++++  G + + N+ +  L P LP WM    CS + + G+ +    + Y    +   I    A++  R+   ++G +++GI +I +P ++    + +P  G+   +G VD  ++PL   L   RH S    V ++   +  +   +     G I    GF +L   +GII+++F+P+  + R    + +K
Sbjct:  276 AGLALFDGALQMLVLQPSKSEPESQTGTPLLTLLRDPYILITAGSICIANMGIAMLEPALPIWMMETMCSPKWQLGVAFIPASISY---WIGTNIFGPLAHKMGRWLCALIGMVVVGISIICVPLAKTIYGLIVPNFGVGFAIGMVDSSMMPLMGYLVDLRHVSVYGSVYAIADVAFCMGFALGPSAGGAIARTIGFPWLMTIIGIIDILFAPLCYFLRSPPGREEK 499
[Close]
BLAST of slc18a-8 vs. Ensembl Medaka
Match: slc18a3b (probable vesicular acetylcholine transporter-B [Source:NCBI gene;Acc:101163117])

HSP 1 Score: 72.4034 bits (176), Expect = 3.830e-13
Identity = 50/205 (24.39%), Postives = 101/205 (49.27%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKL 1423
            F   +R+       IYK + DP++ +  G L V N+ +  L PT+ +WM +   S++ E G+ W   F  +++GV   + +       +++   +G ++IG     +P  + +  +  P+ G+  G+  VD  ++P    L   RH S    V ++   S +++  +  ++ G IV   GF  L + +G++N++++P  L  R +
Sbjct:  248 FSNRTRENMPVGTPIYKLMVDPYIAVVAGALTVANIPLAFLEPTIANWMETTMHSTQWEMGLTWLPAFFPHVLGVYITVKLAAKNPHLQWFYGALGMVIIGASSCTVPACKTFGQLIAPLCGICFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYAMGPIVAGQIVHTLGFVQLNLGMGLVNVLYAPALLLLRTV 452
[Close]
BLAST of slc18a-8 vs. Ensembl Medaka
Match: slc18a3a (solute carrier family 18 member 3a [Source:NCBI gene;Acc:559347])

HSP 1 Score: 65.855 bits (159), Expect = 3.846e-11
Identity = 48/206 (23.30%), Postives = 97/206 (47.09%), Query Frame = 2 
Query:  809 FVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLD 1426
            F    R        IYK + DP++ +  G L + N+ +  L PT+ +WM     ++E E G+ W   F  +++GV   + +       +++   +G + IG     +P  +++  + +P+ G+  G+  VD  ++P    L   RH S    V ++   S  ++  +  ++ G IV   GF  L + +G+ N++++P  L  + + 
Sbjct:  250 FSNQERGNMPVGTPIYKLMIDPYIAVVAGALIICNIPLAFLEPTIANWMEDTMQATEWEIGMTWFPAFFPHVLGVYLTVKLAAKHPHLQWFYGAMGMVFIGASSCTVPACKNFGQLMIPLCGVCFGIALVDTALLPTLGFLVDVRHVSVYGSVYAIADISYCVAYALGPVVAGQIVHNLGFVQLNLGMGLANVLYAPALLLLKNVS 455
[Close]
BLAST of slc18a-8 vs. Ensembl Medaka
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:101172172])

HSP 1 Score: 56.225 bits (134), Expect = 4.043e-8
Identity = 47/197 (23.86%), Postives = 96/197 (48.73%), Query Frame = 2 
Query:  851 IYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            +   ++DP++++  G L   N+ +  L PTLP WM    CS + + G+ +    + Y++G     ++       R+   ++G +++GI ++ +PF+     +  P  G+   +G VD  ++ +   L   RH S    V ++   +L +   I     G +V   GF  L + +G+IN++++P+ L  R    + +K
Sbjct:  241 LLTLLKDPYILISAGSLCFANMGVAILEPTLPIWMMQTMCSPKWQLGVAFLPASISYLIGTNLFGVLANK--MGRWLCSMLGMVIVGISLLCVPFATSIYGLIGPNGGLGFAIGMVDSSMMAIMGYLVDIRHASVYGTVYAIADVALCMGFAIGPSTGGALVQAVGFPCLMVFIGVINILYAPLCLLLRNPAVREEK 435
[Close]
BLAST of slc18a-8 vs. Ensembl Medaka
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:101172172])

HSP 1 Score: 56.225 bits (134), Expect = 4.326e-8
Identity = 47/197 (23.86%), Postives = 96/197 (48.73%), Query Frame = 2 
Query:  851 IYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDK 1441
            +   ++DP++++  G L   N+ +  L PTLP WM    CS + + G+ +    + Y++G     ++       R+   ++G +++GI ++ +PF+     +  P  G+   +G VD  ++ +   L   RH S    V ++   +L +   I     G +V   GF  L + +G+IN++++P+ L  R    + +K
Sbjct:  308 LLTLLKDPYILISAGSLCFANMGVAILEPTLPIWMMQTMCSPKWQLGVAFLPASISYLIGTNLFGVLANK--MGRWLCSMLGMVIVGISLLCVPFATSIYGLIGPNGGLGFAIGMVDSSMMAIMGYLVDIRHASVYGTVYAIADVALCMGFAIGPSTGGALVQAVGFPCLMVFIGVINILYAPLCLLLRNPAVREEK 502
[Close]
BLAST of slc18a-8 vs. Planmine SMEST
Match: SMESG000064367.1 (SMESG000064367.1)

HSP 1 Score: 768.459 bits (1983), Expect = 0.000e+0
Identity = 446/447 (99.78%), Postives = 447/447 (100.00%), Query Frame = 2 
Query:  110 MVLQENSLYAILPKYFSNQTSISARNETVLINSTVPQNCSFQKPPYVVVSQIFLSKNVAMXXXXXXXXXXXXXXXXXXXXXXSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDKNVN 1450
            MVLQENSLYAILPKYFSNQTSISARNETVLINSTVPQNCSFQKPPYVVVSQIFLSKNVAMIFLNIFIGIIIYKLNIYIILILSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDER+GKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYIVGVVTVIIVDKHAYRFRYYVPVVGHLMIGILMIILPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNIVSSLTSQSLTISSVISTLLCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDKNVN
Sbjct:    1 MVLQENSLYAILPKYFSNQTSISARNETVLINSTVPQNCSFQKPPYVVVSQIFLSKNVAMIFLNIFIGIIIYKLNIYIILILSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERYGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYIVGVVTVIIVDKHAYRFRYYVPVVGHLMIGILMIILPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNIVSSLTSQSLTISSVISTLLCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLDFQPDKNVN 447
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BLAST of slc18a-8 vs. Planmine SMEST
Match: SMESG000069017.1 (SMESG000069017.1)

HSP 1 Score: 359.762 bits (922), Expect = 1.619e-118
Identity = 225/468 (48.08%), Postives = 325/468 (69.44%), Query Frame = 2 
Query:   53 FFKFDNLLAGVVVSLNFFLMVLQENSLYAILPKYFSNQTSISARNETVLINSTVPQNCSFQKPPYVVVSQIFLSKNVAMXXXXXXXXXXXXXXXXXXXXXXSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWR----NRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAK-----MDIFVQNSRKKT-KTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRKLD 1426
            F + D LL  +VV LNFF+MV++E+SLY ILPKY SN+T     N + +  S +   C  + PPYV++S IFLSKN+A IF+N  IG+I+ ++N +IILI +  +G+ V  +YA+++++L+YIIGR++D+F+T  I + +Y +IS++F+   +R +YY  VN ++F+SHS+GP YGA VDE+FGKS VFL+L+P+W + LL T  L+ R    N+N+          +    N   +D   NE+   IL ++      + + ++N +    +TL  I+K ++DP V+ CL Q+FVVNL M+SLPPTLPSWMA + CSSE++QGIVW+FGFLGYI+GV  VII+D +A RFR   PV G ++IGI +I++PF+RDW+ +FLPI+GMY+ VG V+QI++PLT  L+   + SN   +SSLTSQSL + SVIS+L  G I   +GF  L I LGIINL FSPISL +RK++
Sbjct:   95 FLRSDYLLVIIVVGLNFFMMVIEESSLYTILPKYLSNRTFGEINNYSRIYASNLSMKCVSKDPPYVIISMIFLSKNLATIFINPIIGMILDRMNNFIILIFAQILGVSVLLVYAFSNSILMYIIGRIIDAFVTSVIFLAVYSIISQMFLETRIRSKYYGFVNTLFFVSHSLGPVYGASVDEKFGKSAVFLLLIPIWSISLLFTYLLHRRIKTINKNKKGVPRFLEIMENHNWNDTRVDSPNNESKNLILSESSEKNETIKVKIENKQSGNHRTLFTIFKMLKDPKVLACLVQIFVVNLCMFSLPPTLPSWMAFKLCSSELQQGIVWSFGFLGYILGVSLVIIIDAYAPRFRNLAPVAGQIIIGIQLIMIPFTRDWRFLFLPIIGMYISVGVVEQIILPLTTYLSDSLYNSNYGTMSSLTSQSLNLCSVISSLCSGPIAEKYGFTTLAIALGIINLSFSPISLIHRKIN 562
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BLAST of slc18a-8 vs. Planmine SMEST
Match: SMESG000041557.1 (SMESG000041557.1)

HSP 1 Score: 306.22 bits (783), Expect = 5.931e-99
Identity = 201/458 (43.89%), Postives = 294/458 (64.19%), Query Frame = 2 
Query:   50 MFFKFDNLLAGVVVSLNFFLMVLQENSLYAILPKYFSNQTSISARNETVLINS--TVPQNCSFQKPPYVVVSQIFLSKNVAMXXXXXXXXXXXXXXXXXXXXXXSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRSE----IKLEGQRQKPNLESLDECINEANVQIL------DKAKMDIFVQNSRKKTKTLNII---YKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGI 1378
            M FK D+ LA +V+ LNF+++ +QE+SLY ILPKY  ++   +  NET   N+  T+ Q C  QKPPY +VS IFLSK++A  F N  IG  + K+N +IIL++   +GI ++ IYA  +++ +YI+GR+LD+F++C + IG + ++SKLF  +  R R++ L N +YF+SHSIGP YGAEVD R GKS VF +L+PLWI  +  T  LY R   R  +S+     KL           LD+  NE  V I+         K   F    + + +   I    YK +++P+V  CL QLF+VNLSM++LP TLPSWM    C++EI+QG+VW FGF+GY+ GV  ++++ K AY+ R++ P++  +++GIL+I+LP S DW+++F+PI+GMY  VG ++Q++ PLT  LA     S+   ++SLTSQSLT+S V+ TL  G I   FGF  L I LGI
Sbjct:    1 MSFKCDDCLAAIVICLNFYMLGIQESSLYTILPKYLVDEPFNATNNETFKPNTSRTIFQ-CHLQKPPYFIVSMIFLSKSLAQFFFNSIIGCFVDKVNNFIILLIGQGLGIIIALIYALNNSITMYIVGRLLDAFVSCSLFIGGFSIVSKLFSNVNTRGRFFGLGNTLYFVSHSIGPVYGAEVDVRLGKSAVFFLLIPLWIFTMAATFILYRRTEKRCNKSKNNCNEKLSIGVTPDETNELDD-HNELKVAIIISSISKTPEKYSDFKGEKKFQKENKEIFMKFYKIMKNPYVSSCLCQLFIVNLSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLIVLLLKCAYQLRFFSPIIAQIIVGILLILLPLSHDWRLLFIPIIGMYFSVGVIEQVLFPLTTYLADHHFNSDYGSMASLTSQSLTLSGVVGTLSSGPIAQRFGFRSLCICLGI 456
[Close]
BLAST of slc18a-8 vs. Planmine SMEST
Match: SMESG000041535.1 (SMESG000041535.1)

HSP 1 Score: 275.018 bits (702), Expect = 7.687e-87
Identity = 200/468 (42.74%), Postives = 283/468 (60.47%), Query Frame = 2 
Query:  107 LMVLQENSLYAILPKYFSNQTSISAR--------------------NETVLINSTVPQNCSFQKPPYVVVSQIFLSKNVAMXXXXXXXXXXXXXXXXXXXXXXSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRNRSKRS-EIKLEGQRQKPNLESLD---------ECINEANVQILDKAKMDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIFSPISLYYRK 1420
            +M + E+SLY ILPKY  +Q +                        N+ + +   + + C  QKPPY +VS +FLSKN+A +  N+ IG II K+N Y ILI +   GI V+ IYA+ ++++ Y+ GRVLD+F+     I I  +IS LF  I  R RY  L N +YF SHSIG  YGAE+D R GKS VF +L+PLWIV + +T+ +Y R +++ K   EIK    ++   +E+L+         E IN  +VQ  DK   D     ++     L  I   ++ P V++CL QLF+VN SM++LP TLPSWM    C++EI+QG+VW FGF+GY+ GV  V +  K+A +F +Y P++  ++IGIL+I+LPFS DW++VF+PI+GMY  VG V+Q++ PLT +LA     S+   +SSLTSQSLTI  V+ TL  G I   FGFH L+I LGI+NL F  +    R 
Sbjct:    1 MMSIPESSLYTILPKYLVDQLNNVTANTTANTTVNATINITFNVTLNDRINLRINISKLCHIQKPPYFIVSMVFLSKNLAQVVSNLIIGCIIEKINHYFILIAAQCFGIVVALIYAFGNSVVAYMCGRVLDAFVNAASYISILAIISSLFSDINTRGRYIGLGNTLYFFSHSIGAVYGAEIDTRLGKSPVFFLLIPLWIVTISITISIYSRVQDKVKYGHEIKKCDDKETIEIENLNNENTSEYPAEVIN--SVQNPDKDS-DFSPMKAKPNKSPLISIMWMLKTPSVLICLIQLFIVNFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYNPIIAQMIIGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSLTICGVMGTLSSGPIAERFGFHTLSIALGILNLTFCGLHFLLRN 465
[Close]
BLAST of slc18a-8 vs. Planmine SMEST
Match: SMESG000041534.1 (SMESG000041534.1)

HSP 1 Score: 201.445 bits (511), Expect = 2.495e-59
Identity = 132/293 (45.05%), Postives = 186/293 (63.48%), Query Frame = 2 
Query:  554 SHSIGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRN---RSKRSEIKLEGQRQKPNLESLDECINEANVQILDKA---------KMDIFVQNSRKKTKT-LNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYXXXXXXXXXXDKHAYRFRYYVPVVGHXXXXXXXXXXPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNXXXXXXXXXXXXXXXXXXXXCGHIVMLFGFHFLTITLGIINLIF 1393
            SHSIG  YGAE+D R GKS VF  L+PLWIV +L+T+ L     N      ++E  ++    K  +E+ +E  +E N + + K          K   F     K  K+ L  I + ++ PFV++CL QLF+ N SM++LP TLPSWM    C++EI+QG+VW FGF+GY+ GV  V +  K+A +F +Y P++  ++ GIL+I+LPFS DW++VF+PI+GMY  VG V+Q++ PLT +LA     S+   +SSLTSQS+TI  V  TL  G I   FGFH L+I+LGI+NLIF
Sbjct:  117 SHSIGAVYGAEIDSRLGKSPVFFFLIPLWIVTILVTILLCKNVSNGFQNDDKNEAGIKTIYNKDIIETNNE--SEGNKKEIQKQVINSDQSPDKDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIF 407

HSP 2 Score: 60.8474 bits (146), Expect = 1.007e-9
Identity = 33/89 (37.08%), Postives = 52/89 (58.43%), Query Frame = 2 
Query:   50 MFFKFDNLLAGVVVSLNFFLMVLQENSLYAILPKYFSNQTSISARNETVLINSTVPQN----------CSFQKPPYVVVSQIFLSKNVA 286
            M F   ++L  ++VSLN F++ + E++LY ILPKYF ++   S  N   +++ TV             C  +KPPY +VS +FL+KN+A
Sbjct:    1 MQFNCGSVLVILMVSLNSFMITIPESTLYTILPKYFVDKHRSSTTNNNTIVDVTVNDTTNLRINISKLCPLKKPPYFMVSMVFLAKNLA 89
The following BLAST results are available for this feature:
BLAST of slc18a-8 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
SLC18A12.291e-1023.35solute carrier family 18 member A1 [Source:HGNC Sy... [more]
SLC18A12.291e-1023.35solute carrier family 18 member A1 [Source:HGNC Sy... [more]
SLC18A27.037e-1024.17solute carrier family 18 member A2 [Source:HGNC Sy... [more]
SLC18A35.762e-924.38solute carrier family 18 member A3 [Source:HGNC Sy... [more]
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BLAST of slc18a-8 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
unc-173.922e-623.44Vesicular acetylcholine transporter unc-17 [Sourc... [more]
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BLAST of slc18a-8 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
VAChT3.853e-1024.54gene:FBgn0270928 transcript:FBtr0083671[more]
Vmat1.075e-620.06gene:FBgn0260964 transcript:FBtr0091492[more]
Vmat1.975e-620.35gene:FBgn0260964 transcript:FBtr0310062[more]
Vmat1.975e-620.35gene:FBgn0260964 transcript:FBtr0091491[more]
Vmat1.975e-620.35gene:FBgn0260964 transcript:FBtr0091493[more]
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BLAST of slc18a-8 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
slc18a3b3.198e-1224.63solute carrier family 18 member 3b [Source:NCBI ge... [more]
slc18a3a7.244e-1224.64solute carrier family 18 member 3a [Source:NCBI ge... [more]
slc18a24.102e-1021.40solute carrier family 18 member 2 [Source:ZFIN;Acc... [more]
slc18a21.102e-922.03solute carrier family 18 member 2 [Source:ZFIN;Acc... [more]
SLC18A13.819e-824.20solute carrier family 18 member A1 [Source:NCBI ge... [more]
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BLAST of slc18a-8 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gtf2e1.21.345e-1124.49general transcription factor IIE, polypeptide 1, a... [more]
gtf2e1.21.437e-1124.62general transcription factor IIE, polypeptide 1, a... [more]
slc18a32.455e-922.44solute carrier family 18 (vesicular acetylcholine ... [more]
kansl34.531e-822.22KAT8 regulatory NSL complex subunit 3 [Source:Xenb... [more]
gtf2e1.24.904e-824.86general transcription factor IIE, polypeptide 1, a... [more]
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BLAST of slc18a-8 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Slc18a24.941e-1024.17solute carrier family 18 (vesicular monoamine), me... [more]
Slc18a31.468e-924.38solute carrier family 18 (vesicular monoamine), me... [more]
Slc18a14.141e-924.21solute carrier family 18 (vesicular monoamine), me... [more]
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BLAST of slc18a-8 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P81721|VACHT_TETCF4.408e-1224.88Vesicular acetylcholine transporter OS=Tetronarce ... [more]
sp|Q91498|VACHT_TORMA1.401e-1125.37Vesicular acetylcholine transporter OS=Torpedo mar... [more]
sp|P59845|VACHB_DANRE2.929e-1124.63Probable vesicular acetylcholine transporter-B OS=... [more]
sp|Q08C75|VACHA_DANRE5.468e-1124.64Probable vesicular acetylcholine transporter-A OS=... [more]
sp|Q91514|VACHT_TORTO7.032e-1124.39Vesicular acetylcholine transporter OS=Torpedo tor... [more]
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BLAST of slc18a-8 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0H3YK880.000e+0100.00Slc18a-8 OS=Schmidtea mediterranea OX=79327 GN=slc... [more]
A0A0H3YJ278.460e-9644.54Slc18a-1 OS=Schmidtea mediterranea OX=79327 GN=slc... [more]
A0A3M7Q6N11.366e-1526.27Vesicular acetylcholine transporter OS=Brachionus ... [more]
A0A267GQ212.063e-1522.76Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267HB058.379e-1523.62Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
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BLAST of slc18a-8 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
slc18a3b4.351e-1323.70solute carrier family 18 member 3b [Source:NCBI ge... [more]
slc18a3a6.605e-1223.90solute carrier family 18 member A3 [Source:NCBI ge... [more]
slc18a25.107e-1122.57synaptic vesicular amine transporter [Source:NCBI ... [more]
SLC18A11.213e-823.35solute carrier family 18 member A1 [Source:NCBI ge... [more]
SLC18A11.416e-822.57solute carrier family 18 member A1 [Source:NCBI ge... [more]
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BLAST of slc18a-8 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
slc18a3a8.128e-1525.12solute carrier family 18 member 3a [Source:NCBI ge... [more]
slc18a25.040e-1223.35solute carrier family 18 member 2 [Source:ZFIN;Acc... [more]
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BLAST of slc18a-8 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of slc18a-8 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of slc18a-8 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
slc18a3b3.830e-1324.39probable vesicular acetylcholine transporter-B [So... [more]
slc18a3a3.846e-1123.30solute carrier family 18 member 3a [Source:NCBI ge... [more]
SLC18A14.043e-823.86solute carrier family 18 member A1 [Source:NCBI ge... [more]
SLC18A14.326e-823.86solute carrier family 18 member A1 [Source:NCBI ge... [more]
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BLAST of slc18a-8 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000064367.10.000e+099.78SMESG000064367.1[more]
SMESG000069017.11.619e-11848.08SMESG000069017.1[more]
SMESG000041557.15.931e-9943.89SMESG000041557.1[more]
SMESG000041535.17.687e-8742.74SMESG000041535.1[more]
SMESG000041534.12.495e-5945.05SMESG000041534.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30035690
GenBankAKN21543.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035690 ID=SMED30035690|Name=slc18a-8|organism=Schmidtea mediterranea sexual|type=transcript|length=1474bp
TGTGATATCTTAATAACTATTGTTAGAATAAATATTAAAAACAAATCTGA
TGTTTTTTAAGTTTGATAATCTACTTGCTGGTGTTGTCGTAAGTTTAAAT
TTCTTTTTAATGGTTTTGCAGGAAAATAGCTTGTACGCGATATTGCCGAA
ATATTTCTCCAATCAAACGTCGATTTCGGCTAGAAATGAAACAGTCCTAA
TTAATTCTACAGTTCCTCAGAATTGTTCCTTTCAAAAACCCCCATATGTT
GTGGTGAGTCAAATATTTTTGTCAAAAAATGTTGCGATGATTTTCTTAAA
CATTTTCATTGGAATTATTATTTATAAATTGAATATTTACATTATTTTGA
TTTTGTCACATTTTGTTGGTATTGGAGTTTCGTTCATTTATGCTTACACG
GACACGTTACTCCTGTACATTATCGGTCGGGTGCTAGATAGTTTTATTAC
TTGTGGAATTTTGATTGGGATTTATTTTCTTATATCTAAACTCTTTATTG
TCATTGAAGTCAGAGATAGATATTACGCTTTAGTAAACATAATTTATTTC
ATTAGCCATTCTATCGGTCCCTTTTATGGAGCTGAAGTCGATGAAAGATT
TGGGAAATCTACTGTCTTTCTGATTTTAGTTCCACTCTGGATTGTGTGTT
TACTGCTAACAGTATTTCTGTATTGGAGAAATCGAAATCGTTCAAAGAGA
TCAGAGATTAAACTAGAAGGCCAACGCCAAAAACCTAATTTAGAGTCACT
AGATGAATGTATAAATGAGGCAAATGTACAAATTCTGGATAAAGCAAAGA
TGGATATATTTGTCCAAAACTCAAGAAAAAAAACTAAAACATTAAATATC
ATTTATAAATTCATTCGAGACCCTTTTGTCATGTTGTGTTTAGGGCAATT
ATTTGTTGTTAATTTGAGTATGTATTCATTGCCGCCAACCTTGCCGTCGT
GGATGGCATCGGAATTCTGTTCATCCGAAATTGAACAAGGTATTGTTTGG
ACATTCGGATTTTTGGGTTACATTGTTGGAGTTGTTACCGTTATTATTGT
TGATAAACATGCTTATAGATTCAGATATTATGTACCCGTAGTAGGTCATT
TGATGATTGGGATTCTGATGATAATTTTACCGTTTAGTCGTGATTGGAAA
ATGGTGTTTTTACCCATTGTTGGAATGTATGTAGGTGTGGGATTTGTGGA
TCAAATTGTGATACCATTAACAATAAATTTGGCAATTTTTCGGCACAAAT
CTAACACAAATATAGTCTCATCCCTCACTAGTCAATCTTTGACTATAAGC
AGCGTCATAAGTACTCTTCTCTGTGGTCATATTGTTATGTTATTCGGATT
CCATTTTTTAACAATAACTCTTGGAATTATTAATTTAATTTTTTCACCGA
TTTCTCTTTATTATCGAAAACTTGACTTTCAACCTGATAAAAATGTAAAT
TAAAAATCATTATTTACAAAGACA
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protein sequence of SMED30035690-orf-1

>SMED30035690-orf-1 ID=SMED30035690-orf-1|Name=SMED30035690-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=468bp
MFFKFDNLLAGVVVSLNFFLMVLQENSLYAILPKYFSNQTSISARNETVL
INSTVPQNCSFQKPPYVVVSQIFLSKNVAMIFLNIFIGIIIYKLNIYIIL
ILSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLFI
VIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLWIVC
LLLTVFLYWRNRNRSKRSEIKLEGQRQKPNLESLDECINEANVQILDKAK
MDIFVQNSRKKTKTLNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPS
WMASEFCSSEIEQGIVWTFGFLGYIVGVVTVIIVDKHAYRFRYYVPVVGH
LMIGILMIILPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHK
SNTNIVSSLTSQSLTISSVISTLLCGHIVMLFGFHFLTITLGIINLIFSP
ISLYYRKLDFQPDKNVN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR011701MFS
IPR020846MFS_dom
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 16..419
e-value: 4.3E-21
score: 75.2
NoneNo IPR availablePANTHERPTHR23506FAMILY NOT NAMEDcoord: 19..455
NoneNo IPR availableCDDcd17383MFS_SLC18A3_VAChTcoord: 268..456
e-value: 3.02845E-18
score: 83.9005
NoneNo IPR availableTMHMMTMhelixcoord: 375..397
NoneNo IPR availableTMHMMTMhelixcoord: 187..209
NoneNo IPR availableTMHMMTMhelixcoord: 341..360
NoneNo IPR availableTMHMMTMhelixcoord: 7..24
NoneNo IPR availableTMHMMTMhelixcoord: 158..177
NoneNo IPR availableTMHMMTMhelixcoord: 123..145
NoneNo IPR availableTMHMMTMhelixcoord: 67..89
NoneNo IPR availableTMHMMTMhelixcoord: 436..455
NoneNo IPR availableTMHMMTMhelixcoord: 409..431
NoneNo IPR availableTMHMMTMhelixcoord: 276..295
NoneNo IPR availableTMHMMTMhelixcoord: 96..118
NoneNo IPR availableTMHMMTMhelixcoord: 315..334
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 14..458