SMED30035644

Overview
NameSMED30035644
Smed IDSMED30035644
Length (bp)4077
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30035644 (SMED30035644) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30035644 (SMED30035644) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30035644 (SMED30035644) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30035644 (SMED30035644) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30035644

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30035644SMESG000014253.1 dd_Smed_v4_12073_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30035644 vs. Planmine SMEST
Match: SMESG000014253.1 (SMESG000014253.1)

HSP 1 Score: 2145.55 bits (5558), Expect = 0.000e+0
Identity = 1124/1126 (99.82%), Postives = 1125/1126 (99.91%), Query Frame = 1
Query:  541 MNWENIQILYIWYCILKLIRSEDISISPYLNAFKIYKKQSDFTLKSFAFINPAVHNFNLTTKLEDDIKDNQFIKSDFSVSIHTDKHQYYDLDKTESYTINTICKFDYIPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMFQKSFIPKPSSADHFYFGLRAFAVATNSDHCTSHSDDMRILAVSKLYPLSFSNGLSVFWTNIFQSNLNFSLEIGRPFYSIDPDSFVSFPIKLISDKMFSQMNLQCIISENLEIKSVFFDENIFDAQYKIAALPNRFIITILLENKGMNYLKSSQWWVDRGLLLFIMYGRITESYMLSRKNDLRTAWASCQIINLKLQGESKSVAYTSKILQTVHIAINTDKFIIIPILKTRYFFSIFNDINRPISLEDDFITLYSIHDVIDGSFVDVTNSAICNSIDKSSIICPLSMMKSSDQYFHTKFDNKSDNGRTRILPNKFDLILEMKTWKTVLFAPINLWVAQDWKLDINVKIINKINGWRVPVRSSVTREIICVTMFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYIPYSNQLNISLYESGSCRFSKLIFTGLVRINIQHSEDIPXXXXXXXXXXXXXXXXNPSTVNTNQIQNRELIKNLSSLYYGXXXXXXXXXXXXXXXXXNCLVKFNYRKISHQSSNALNQSVNNKLLNSAQPPALADTSSPTTSFTKHXXXXXXXXXXRNASNSEVELPSSTFWSYCSSQDSSSFTNRKYSSTLLEPTISISPLNNFLIKTSPLVSSVYTNTKYRNNPSPSSDQGDGGYLSGENQSKITQIPLIKKLSDDRNANLESVDMTSSSSGGLSHYAEXXXXXXXXXXLKWSIQWATSMVSAIPVFSQSYTPNLITDSTCVISRAVPCGEPTSPINQWITNKTIPSSSSVPYTKSIDNLCEKSSGLGSLTSTQQEDYSMLSDDDNYHVSINSDKEHYRKYFKKC 3918
            MNWENIQILYIWYCILKLIRSEDISISPYLNAFKIYKKQSDFTLKSFAFINPAVHNFNLTTKLEDDIKDNQFIKSDFSVSIHTDKHQYYDLDKTESYTINTICKFDYIPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMFQKSFIPKPSSADHFYFGLRAFAVATNSDHCTSHSDDMRILAVSKLYPLSFSNGLSVFWTNIFQSNLNFSLEIGRPFYSIDPDSFVSFPIKLISDKMFSQMNLQCIISENLEIKSVFFDENIFDAQYKIAALPNRFIITILLENKGMNYLKSSQWWVDRGLLLFIMYGRITESYMLSRKNDLRTAWASCQIINLKLQGESKSVAYTSKILQTVHIAINTDKFIIIPILKTRYFFSIFNDINRPISLEDDFITLYSIHDVIDGSFVDVTNSAICNSIDKSSIICPLSMMKSSDQYFHTKFDNKSDNGRTRILPNKFDLILEMKTWKTVLFAPINLWVAQDWKLDINVK+INKINGWRVPVRSSVTREIICVTMFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYI YSNQLNISLYESGSCRFSKLIFTGLVRINIQHSEDIPTTISSTMISTEITSTINPSTVNTNQIQNRELIKNLSSLYYGLYALIALVSLVALIVLVNCLVKFNYRKISHQSSNALNQSVNNKLLNSAQPPALADTSSPTTSFTKHPPLLPPPGPLRNASNSEVELPSSTFWSYCSSQDSSSFTNRKYSSTLLEPTISISPLNNFLIKTSPLVSSVYTNTKYRNNPSPSSDQGDGGYLSGENQSKITQIPLIKKLSDDRNANLESVDMTSSSSGGLSHYAEPYPVPPPCPPLKWSIQWATSMVSAIPVFSQSYTPNLITDSTCVISRAVPCGEPTSPINQWITNKTIPSSSSVPYTKSIDNLCEKSSGLGSLTSTQQEDYSMLSDDDNYHVSINSDKEHYRKYFKKC
Sbjct:    1 MNWENIQILYIWYCILKLIRSEDISISPYLNAFKIYKKQSDFTLKSFAFINPAVHNFNLTTKLEDDIKDNQFIKSDFSVSIHTDKHQYYDLDKTESYTINTICKFDYIPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMFQKSFIPKPSSADHFYFGLRAFAVATNSDHCTSHSDDMRILAVSKLYPLSFSNGLSVFWTNIFQSNLNFSLEIGRPFYSIDPDSFVSFPIKLISDKMFSQMNLQCIISENLEIKSVFFDENIFDAQYKIAALPNRFIITILLENKGMNYLKSSQWWVDRGLLLFIMYGRITESYMLSRKNDLRTAWASCQIINLKLQGESKSVAYTSKILQTVHIAINTDKFIIIPILKTRYFFSIFNDINRPISLEDDFITLYSIHDVIDGSFVDVTNSAICNSIDKSSIICPLSMMKSSDQYFHTKFDNKSDNGRTRILPNKFDLILEMKTWKTVLFAPINLWVAQDWKLDINVKVINKINGWRVPVRSSVTREIICVTMFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYILYSNQLNISLYESGSCRFSKLIFTGLVRINIQHSEDIPTTISSTMISTEITSTINPSTVNTNQIQNRELIKNLSSLYYGLYALIALVSLVALIVLVNCLVKFNYRKISHQSSNALNQSVNNKLLNSAQPPALADTSSPTTSFTKHPPLLPPPGPLRNASNSEVELPSSTFWSYCSSQDSSSFTNRKYSSTLLEPTISISPLNNFLIKTSPLVSSVYTNTKYRNNPSPSSDQGDGGYLSGENQSKITQIPLIKKLSDDRNANLESVDMTSSSSGGLSHYAEPYPVPPPCPPLKWSIQWATSMVSAIPVFSQSYTPNLITDSTCVISRAVPCGEPTSPINQWITNKTIPSSSSVPYTKSIDNLCEKSSGLGSLTSTQQEDYSMLSDDDNYHVSINSDKEHYRKYFKKC 1126          

HSP 2 Score: 239.58 bits (610), Expect = 2.891e-64
Identity = 138/167 (82.63%), Postives = 140/167 (83.83%), Query Frame = -2
Query:    3 MNWENIQILYIWYCILKLIRSEDISISPYLNAXX------------------------XXXXXXXXXXXXXXXKSDFSVSIHTDKHQYYDLDKTESYTINTICKFDYIPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMFQKSFIPKPSS 431
            MNWENIQILYIWYCILKLIRSEDISISPYLNAFK                        +  K +DDIKDNQFIKSDFSVSIHTDKHQYYDLDKTESYTINTICKFDYIPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMFQKSFIPKPSS
Sbjct:    1 MNWENIQILYIWYCILKLIRSEDISISPYLNAFKIYKKQSDFTLKSFAFINPAVHNFNLTTKLEDDIKDNQFIKSDFSVSIHTDKHQYYDLDKTESYTINTICKFDYIPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMFQKSFIPKPSS 167          
BLAST of SMED30035644 vs. Planmine SMEST
Match: SMESG000014253.1 (SMESG000014253.1)

HSP 1 Score: 1063.91 bits (2750), Expect = 0.000e+0
Identity = 598/599 (99.83%), Postives = 598/599 (99.83%), Query Frame = 1
Query: 2122 MFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYIPYSNQLNISLYESGSCRFSKLIFTGLVRINIQHSEDIPXXXXXXXXXXXXXXXXNPSTVNTNQIQNRELIKNLSSLYYGXXXXXXXXXXXXXXXXXNCLVKFNYRKISHQSSNALNQSVNNKLLNSAQPPALADTSSPTTSFTKHXXXXXXXXXXRNASNSEVELPSSTFWSYCSSQDSSSFTNRKYSSTLLEPTISISPLNNFLIKTSPLVSSVYTNTKYRNNPSPSSDQGDGGYLSGENQSKITQIPLIKKLSDDRNANLESVDMTSSSSGGLSHYAEXXXXXXXXXXLKWSIQWATSMVSAIPVFSQSYTPNLITDSTCVISRAVPCGEPTSPINQWITNKTIPSSSSVPYTKSIDNLCEKSSGLGSLTSTQQEDYSMLSDDDNYHVSINSDKEHYRKYFKKC 3918
            MFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYI YSNQLNISLYESGSCRFSKLIFTGLVRINIQHSEDIPTTISSTMISTEITSTINPSTVNTNQIQNRELIKNLSSLYYGLYALIALVSLVALIVLVNCLVKFNYRKISHQSSNALNQSVNNKLLNSAQPPALADTSSPTTSFTKHPPLLPPPGPLRNASNSEVELPSSTFWSYCSSQDSSSFTNRKYSSTLLEPTISISPLNNFLIKTSPLVSSVYTNTKYRNNPSPSSDQGDGGYLSGENQSKITQIPLIKKLSDDRNANLESVDMTSSSSGGLSHYAEPYPVPPPCPPLKWSIQWATSMVSAIPVFSQSYTPNLITDSTCVISRAVPCGEPTSPINQWITNKTIPSSSSVPYTKSIDNLCEKSSGLGSLTSTQQEDYSMLSDDDNYHVSINSDKEHYRKYFKKC
Sbjct:    1 MFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYILYSNQLNISLYESGSCRFSKLIFTGLVRINIQHSEDIPTTISSTMISTEITSTINPSTVNTNQIQNRELIKNLSSLYYGLYALIALVSLVALIVLVNCLVKFNYRKISHQSSNALNQSVNNKLLNSAQPPALADTSSPTTSFTKHPPLLPPPGPLRNASNSEVELPSSTFWSYCSSQDSSSFTNRKYSSTLLEPTISISPLNNFLIKTSPLVSSVYTNTKYRNNPSPSSDQGDGGYLSGENQSKITQIPLIKKLSDDRNANLESVDMTSSSSGGLSHYAEPYPVPPPCPPLKWSIQWATSMVSAIPVFSQSYTPNLITDSTCVISRAVPCGEPTSPINQWITNKTIPSSSSVPYTKSIDNLCEKSSGLGSLTSTQQEDYSMLSDDDNYHVSINSDKEHYRKYFKKC 599          
BLAST of SMED30035644 vs. Planmine SMEST
Match: SMESG000014253.1 (SMESG000014253.1)

HSP 1 Score: 1040.02 bits (2688), Expect = 0.000e+0
Identity = 586/587 (99.83%), Postives = 586/587 (99.83%), Query Frame = 1
Query: 2158 MVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYIPYSNQLNISLYESGSCRFSKLIFTGLVRINIQHSEDIPXXXXXXXXXXXXXXXXNPSTVNTNQIQNRELIKNLSSLYYGXXXXXXXXXXXXXXXXXNCLVKFNYRKISHQSSNALNQSVNNKLLNSAQPPALADTSSPTTSFTKHXXXXXXXXXXRNASNSEVELPSSTFWSYCSSQDSSSFTNRKYSSTLLEPTISISPLNNFLIKTSPLVSSVYTNTKYRNNPSPSSDQGDGGYLSGENQSKITQIPLIKKLSDDRNANLESVDMTSSSSGGLSHYAEXXXXXXXXXXLKWSIQWATSMVSAIPVFSQSYTPNLITDSTCVISRAVPCGEPTSPINQWITNKTIPSSSSVPYTKSIDNLCEKSSGLGSLTSTQQEDYSMLSDDDNYHVSINSDKEHYRKYFKKC 3918
            MVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYI YSNQLNISLYESGSCRFSKLIFTGLVRINIQHSEDIPTTISSTMISTEITSTINPSTVNTNQIQNRELIKNLSSLYYGLYALIALVSLVALIVLVNCLVKFNYRKISHQSSNALNQSVNNKLLNSAQPPALADTSSPTTSFTKHPPLLPPPGPLRNASNSEVELPSSTFWSYCSSQDSSSFTNRKYSSTLLEPTISISPLNNFLIKTSPLVSSVYTNTKYRNNPSPSSDQGDGGYLSGENQSKITQIPLIKKLSDDRNANLESVDMTSSSSGGLSHYAEPYPVPPPCPPLKWSIQWATSMVSAIPVFSQSYTPNLITDSTCVISRAVPCGEPTSPINQWITNKTIPSSSSVPYTKSIDNLCEKSSGLGSLTSTQQEDYSMLSDDDNYHVSINSDKEHYRKYFKKC
Sbjct:    1 MVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYILYSNQLNISLYESGSCRFSKLIFTGLVRINIQHSEDIPTTISSTMISTEITSTINPSTVNTNQIQNRELIKNLSSLYYGLYALIALVSLVALIVLVNCLVKFNYRKISHQSSNALNQSVNNKLLNSAQPPALADTSSPTTSFTKHPPLLPPPGPLRNASNSEVELPSSTFWSYCSSQDSSSFTNRKYSSTLLEPTISISPLNNFLIKTSPLVSSVYTNTKYRNNPSPSSDQGDGGYLSGENQSKITQIPLIKKLSDDRNANLESVDMTSSSSGGLSHYAEPYPVPPPCPPLKWSIQWATSMVSAIPVFSQSYTPNLITDSTCVISRAVPCGEPTSPINQWITNKTIPSSSSVPYTKSIDNLCEKSSGLGSLTSTQQEDYSMLSDDDNYHVSINSDKEHYRKYFKKC 587          
BLAST of SMED30035644 vs. Planmine SMEST
Match: SMESG000036221.1 (SMESG000036221.1)

HSP 1 Score: 186.422 bits (472), Expect = 1.884e-47
Identity = 158/678 (23.30%), Postives = 311/678 (45.87%), Query Frame = 1
Query:  592 LIRSEDISISPYLNAFKIYKKQSDFTLKSFAFINPAVHNFNLTTKLEDDIKDNQFIKSDFSVSIHTDKHQY--YDLDKTESYTINTICKFDYIPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMF-QKSFIPKPSSADHFYFGLRAFAVATNSDHCTSHSDDMRILAVSKLYPLSFSNGLSVFWTNIFQSNLNFSLEIGRPFYSIDPDSFVSFPIKLISDKMFSQMNLQCIISENLEIKSVFFDENIFDAQY-KIAALPNRFII--TILLENKGMNYLKSSQWWVDRGLLLFIMYGRITESYMLSRKNDLRTAWASCQIINLKL-QGESKSVAYTSKILQTVHIAINTDKFIIIPILKTRYFFSIFNDINRPISLEDDFITLYSIHDVIDGSFVDVTNSAICNSIDKSSIICPLSMMKSSDQYFHTKFDNKSDNGRTRILPNKFDLILEMKTWKTVLFAPINLWVAQDWKLDINVKIINKINGWRVPVRSSVTREIICVTMFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQS-DVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRL 2601
            L     I+I+P  + F  Y  +     +   + NP  +  N++    +  K       ++  ++ TDK ++       T++ T+  IC+F +   + + +  +CC + ++    T++  +  K+C+  +++ +S C +      ++F  K S+ +      R FA  +    C+S +  +  LA+     L+    L V W  I  ++    L IG P  S++P S   FP+K+ S  +++ + +QC    +L++ S+ FD   F     KI       +I  +  LEN G            + ++  ++Y RI     + +K+D    W SC+II     + +SK    T++  + + I +N   F I PILK   +FSI + +  PI +  + + + S H++ D SF D+T +  C  +  S + CP  + K+S++    K   K D          ++++L +++WK  +  PI LW A DW+L    + +NKI+GW             C  +FQ +R K+L R++      ++ +YG +  +  P LD+T  + K +I+ S ++  +     F  L    QS  +   E+ +    S + +A + ++    +V I +++  C+  + + Q  +     + ++ V   L  D+ + CY  I++  +D+ +  L
Sbjct:   15 LAECTTINITPLRSGFVKYPGEFSDNYE-IIYKNPLENKMNISVLFGNLTKTKNLEPLNYITNVFTDKTRFSIQSNQLTDTSTVYAICQFTHGNSRPFEYNKVCCEMILKRLG-TNSKIFDRKYCQMDVNKDTSVCSVQFQIPHENFEFKTSTEEQLDISSRVFA--SQDGKCSSTAIGVSNLALGYPTKLTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVYNSLAIQCSHGRSLKLSSISFDSVRFTGTLQKITDTKKEILIEESNSLENSG-----------GQRIVELLVYLRIHHLNSIPKKSD---NWISCKIIQASFDKNKSKKSRKTTEKEKKISIQVNIGHFYIFPILKRSIYFSILDTVMGPIEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPF-VRKTSNE-ISKKIIRKPD----------WNVVLSLRSWKKTVTVPIELWQADDWRLTTTDRTLNKISGWNYK-----NSNYQCEPVFQVTRCKLLARLMKDTFDSKRSYYGQSELDKIPLLDMTPYLRKQNIKLSTNNFNVDLRNDFMLL----QSVQIGSSEISIFDNHSSK-IAFDFIQADAQEVSIVDMEVSCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILLTFNDNKKSNL 652          
BLAST of SMED30035644 vs. Planmine SMEST
Match: SMESG000068172.1 (SMESG000068172.1)

HSP 1 Score: 131.339 bits (329), Expect = 1.612e-30
Identity = 179/737 (24.29%), Postives = 317/737 (43.01%), Query Frame = 1
Query:  673 KSFAFINPAVHNFNLTTKLED---DIKDNQFIKSDFSVSIHTDKHQYYDLDKTESYTINTICKFDY---IPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMFQKSFIPKPSSADHFYFGLRAFAVATNSDHCTSHSDDMRILAVSKLYPLSFSNGLSVFWTNIFQSNLNFSLEIGRPFYSIDPDSFVSFPIKLISD--KMFSQM-NLQCIISENLEIKSVFFD-ENIFDAQYKIAALPNRFIITILLENKGMNYLKSSQWWVDRGLLLFIMYGRITESYMLSRKNDLRTAWASCQIINLKLQGESKSVAYTSKILQTVHIAINTDKFIIIPILK-TRYFFSIFNDINRPISLEDDFITLYSIHDVIDGSFVDVTNSAICNSIDKSSIICPLSMMKSSDQYFHTKFDNKSDNGRTRILPNKFDLILEMKTWKTVLFAPINLWVAQDWKLDINVKIINKINGWRVPVRSSVTREIICVTMFQRSRLKVLVRMVNRNTLGEKEFYGST--SSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSE---LIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKS-----DIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHY--IQVPFVVSRYIPYSNQLNISLYESGSCRFSKLIFTGLVR-INIQHSEDI 2811
            + F   N  + N N+T KL D    +  N+  +S ++V I  ++  Y     +    INTIC+F Y   I  ++ L  + CCR+ +  ++ + +       C  SL R    CFI + F  S        +     L       +S  C    +   I  VSK   +SFS+ L V W          ++ IG P YS+     +  P++L+SD   + +Q+  + C +  N    SV  + EN+   +       N  I+ + L+             +   L   +M+  +T       KN L+     C+II      + K+  Y       + +  + D  II+P++K +R  FSI++  N  I  ++D + LY I +  D S   +++      +D      P+ +   S+       D    N +T +  N + + ++       L +P+ +W  + WKL ++ +I+  I  W V    S      CV  ++ +RLK++ R+      GEK + G+    +N  P++D+T+    N I+F P   I S ++  ++   LI   Q+D +   ++L S  S+  L  +++      + + ++   C+ +L L Q K      +  S Y+ + V  +      K CYL + VK SD S  RL  I    Y+  +T  D     Y       V  R++P   ++N+SLYE  SC   +LIF+  +  + ++ S +I
Sbjct:   52 QRFVIFNSEI-NQNITVKLGDLQSALPLNEKSES-YNVKIFAERRGYLPPSNSNEIVINTICQFTYSLAIMSRLEL--DFCCRVELLIYSKSKSILNPFGVCDLSLRRDEVHCFIPIKFVYS-----QYLNEKILPLIGIVHKKSSGSCHFQPEPKDIYKVSKGLGISFSSRLPVIWQPTHSQK--STILIGIPLYSVKDYENLLIPVRLVSDLVDLTNQIIRINCQLDPNFVFHSVIVNSENVSIIEDPEIEDENWTIVDVKLK-------------ITPSLDQQLMFLNVTVKLDSMTKNSLKFIKVKCKII--PRNNKHKARLYDDATEVKIPLRTSND-LIILPVVKESRDIFSIYSKPN--IDFQNDPLQLYIITNYRDRSVKKISSKFQMYPVD------PIHLSVHSNNQSLMSMDM---NYQTYL--NPWSIQVQFIDNLHTLISPVRVWRPESWKLMLSDEILRPIKNWAVYQPDSEE----CVPRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQIKFYPED-IFSIEKSLNQEYFLITAKQADKSS-SIKLFSEYSQTPLTSKQISADQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVS-LTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTNPDSDMFIYKITDNQLVTVRHMP---RVNMSLYEDNSCSPPRLIFSKKIEWVTVEPSNNI 738          
The following BLAST results are available for this feature:
BLAST of SMED30035644 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035644 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014253.12.891e-6499.82SMESG000014253.1[more]
SMESG000014253.10.000e+099.83SMESG000014253.1[more]
SMESG000014253.10.000e+099.83SMESG000014253.1[more]
SMESG000036221.11.884e-4723.30SMESG000036221.1[more]
SMESG000068172.11.612e-3024.29SMESG000068172.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035644 ID=SMED30035644|Name=SMED30035644|organism=Schmidtea mediterranea sexual|type=transcript|length=4077bp
GCCGAACTAGGTTTTGGTATGAAAGACTTCTGGAACATCAAATCAATGAA
ACATGCACTTGAATGACGACTGAGGCTGAATTTACAGAATTTAATAGAAT
AGAAATCATTACTGGTAAAATTAAAATCCTGAATTGTTATTCGACAACAA
ATATTTCGAAAAAGATAAATTTTCTCTGGAATATAATCGAATTTACATAT
AGTATTAATTGTATAAGATTCGGTTTTATCTAAATCATAGTATTGATGTT
TATCAGTGTGAATTGATACTGAGAAATCAGATTTAATGAATTGATTATCT
TTGATATCGTCTTTTTGCTTTTTATAAATTTTAAATGCATTCAAATAAGG
AGAAATTGATATATCTTCAGAACGAATTAATTTTAATATACAATACCATA
TGTATAAAATTTGAATATTTTCCCAATTCATTTTTAGATCAAAATAAATT
TTAAATTGTTCTAATAACCTTCAATTAATTCAACTATGATAATTTATTGA
ATAGTTATTAGAACAATTTAAAATTTATTTTGATCTAAAAATGAATTGGG
AAAATATTCAAATTTTATACATATGGTATTGTATATTAAAATTAATTCGT
TCTGAAGATATATCAATTTCTCCTTATTTGAATGCATTTAAAATTTATAA
AAAGCAAAGTGATTTTACTCTTAAAAGTTTTGCATTTATTAATCCTGCTG
TTCATAATTTTAATCTTACCACAAAATTAGAAGACGATATCAAAGATAAT
CAATTCATTAAATCTGATTTCTCAGTATCAATTCACACTGATAAACATCA
ATACTATGATTTAGATAAAACCGAATCTTATACAATTAATACTATATGTA
AATTCGATTATATTCCAGAGAAAATTTATCTTTTTCGAAATATTTGTTGT
CGAATAACAATTCAGGATTTTAATTTTACCAGTAATGATTTCTATTCTAT
TAAATTCTGTAAATTCAGCCTCAGTCGTCATTCAAGTGCATGTTTCATTG
ATTTGATGTTCCAGAAGTCTTTCATACCAAAACCTAGTTCGGCAGATCAT
TTCTATTTTGGCTTAAGAGCCTTTGCAGTTGCTACCAACAGCGACCATTG
CACTTCTCATTCCGATGATATGAGAATTCTCGCTGTTAGCAAACTCTATC
CTTTGTCATTCAGTAATGGATTAAGTGTGTTTTGGACAAATATTTTTCAA
TCGAATTTGAATTTTTCTCTTGAAATTGGACGTCCATTTTATTCAATTGA
TCCTGATAGTTTTGTATCTTTTCCAATAAAATTAATTTCTGATAAAATGT
TTAGTCAAATGAACCTCCAATGTATTATCAGTGAGAATTTAGAGATTAAA
TCAGTTTTTTTCGATGAGAACATATTCGACGCTCAATACAAAATTGCGGC
ACTCCCAAACCGATTCATAATAACAATACTTCTCGAGAATAAAGGAATGA
ATTATCTGAAATCGTCACAATGGTGGGTTGATCGCGGCTTGCTGCTGTTT
ATTATGTACGGTAGAATTACAGAATCGTATATGCTCTCTAGAAAAAATGA
TCTGCGAACTGCATGGGCTAGTTGTCAAATAATAAATCTCAAACTTCAAG
GAGAATCCAAATCGGTTGCATATACATCAAAGATATTACAGACAGTCCAT
ATCGCTATAAACACAGATAAATTTATAATCATACCAATTTTAAAAACGAG
ATATTTTTTTTCAATTTTCAACGATATTAACCGTCCAATAAGTCTTGAAG
ACGATTTCATTACTTTATACAGTATTCATGACGTTATTGACGGCTCGTTT
GTTGATGTGACAAATTCTGCAATTTGCAACTCTATAGATAAAAGCAGCAT
TATTTGTCCACTTTCCATGATGAAGTCATCAGATCAGTATTTCCACACGA
AATTTGACAATAAATCCGACAACGGCCGAACTCGTATTCTTCCAAATAAA
TTTGACTTGATTTTAGAAATGAAAACTTGGAAAACCGTTCTATTTGCTCC
AATAAACCTTTGGGTTGCTCAAGACTGGAAGCTTGATATTAATGTCAAAA
TTATTAATAAAATTAACGGTTGGAGAGTGCCTGTCAGATCATCAGTTACG
AGAGAAATTATTTGCGTTACAATGTTTCAAAGAAGTCGGTTAAAGGTTTT
AGTGAGAATGGTAAATCGCAATACATTGGGAGAAAAAGAATTCTATGGCA
GCACCAGTTCTAATGGTTCCCCATTTCTTGATATTACATCGCAAATTCCA
AAAAATTCAATAGAATTTTCACCATCTAGCAAAATTATTTCATACGACGA
AAAATTTTCCGAACTCATTGTCGATCCTCAGTCTGACGTTAATGATGTTG
AAGTTCGACTTTTGTCAGTCGACAGTAAACGACTCCTAGCAATCGAGCGA
GTAAAGATTCGTCTAGATAAAGTGATAATTGCAAACATAAAAGCATTCTG
TGTTTCAAGTCTTGATTTATTTCAAGATAAAAGTGACATTTATTCTAACT
ATCGTCGACTTCATGTGGAATACATACTTCAAAACGACACTAGAAAAATG
TGTTATTTATTTATTGTTGTTAAATTGTCAGATTCGTCGGAAATCCGCCT
CGACGAAATTCCTAGTAACTATTACCGAGCCTTCATTACCCAATATGATT
ATTCAAGCAAACACTATATTCAGGTTCCATTTGTAGTATCAAGATACATC
CCCTATTCCAATCAACTGAATATTAGCCTCTATGAATCTGGTAGTTGTCG
TTTTTCTAAATTAATATTTACAGGACTTGTAAGAATAAATATTCAGCATA
GTGAAGATATTCCAACAACCATATCTTCAACAATGATTTCAACAGAAATA
ACCTCTACAATCAACCCAAGTACAGTAAATACTAACCAAATACAGAATAG
AGAACTGATAAAGAATCTTTCATCTCTCTATTATGGCTTGTATGCACTTA
TAGCACTCGTTTCTCTTGTTGCTCTCATTGTTCTAGTCAATTGTCTCGTA
AAATTTAATTATCGCAAAATATCCCACCAAAGTAGCAATGCACTAAATCA
ATCGGTAAACAATAAATTGCTCAACTCAGCTCAACCACCAGCATTAGCTG
ATACATCATCACCTACCACTAGTTTCACTAAACATCCTCCATTATTGCCT
CCTCCAGGTCCGTTAAGAAATGCGTCGAATTCAGAAGTCGAATTACCATC
AAGTACATTTTGGAGCTATTGCTCATCTCAAGATTCATCCAGTTTTACTA
ATAGGAAATATTCATCAACGCTTCTTGAACCAACAATTTCGATCTCTCCA
CTTAACAATTTTCTAATTAAGACGAGTCCCCTTGTGTCTTCAGTTTATAC
AAATACAAAATATAGGAACAATCCATCACCATCTAGTGATCAAGGAGACG
GGGGTTATTTGAGTGGAGAAAATCAAAGCAAAATTACTCAGATTCCTTTA
ATAAAAAAATTATCAGATGATAGAAATGCTAATCTAGAATCAGTCGACAT
GACCAGCAGTAGCAGTGGTGGTTTGTCTCATTATGCAGAACCATATCCGG
TTCCTCCACCTTGTCCCCCATTAAAATGGTCAATACAGTGGGCGACAAGT
ATGGTTTCGGCCATACCAGTTTTCTCTCAATCCTATACCCCAAATTTAAT
AACCGACAGTACATGTGTCATTTCCCGAGCAGTTCCCTGTGGTGAACCAA
CTTCACCAATAAATCAGTGGATCACCAACAAAACGATACCGTCCTCGAGT
TCTGTTCCATATACGAAATCTATTGATAATCTTTGCGAAAAAAGCAGTGG
ACTAGGCAGCTTAACAAGTACCCAACAAGAAGATTATTCTATGTTATCGG
ACGATGACAATTATCACGTCTCTATCAACAGTGATAAAGAGCACTATAGA
AAATACTTTAAGAAATGTTAAAATAAAGATTAAAACAAAAGTTGAAATAT
TTAATATCCATTGATGTTCAAATTACTTTCTCAATCATTTAATTTTGTAT
ATATGTAAATAACTTTTGAAGCTGAATTTTTTACTTGTGCTGTTGATTCG
GTGTAAATTTGGAATCGTTTTTGCCAT
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protein sequence of SMED30035644-orf-1

>SMED30035644-orf-1 ID=SMED30035644-orf-1|Name=SMED30035644-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=143bp
MNWENIQILYIWYCILKLIRSEDISISPYLNAFKIYKKQKDDIKDNQFIK
SDFSVSIHTDKHQYYDLDKTESYTINTICKFDYIPEKIYLFRNICCRITI
QDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMFQKSFIPKPSS
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protein sequence of SMED30035644-orf-2

>SMED30035644-orf-2 ID=SMED30035644-orf-2|Name=SMED30035644-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1127bp
MNWENIQILYIWYCILKLIRSEDISISPYLNAFKIYKKQSDFTLKSFAFI
NPAVHNFNLTTKLEDDIKDNQFIKSDFSVSIHTDKHQYYDLDKTESYTIN
TICKFDYIPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSA
CFIDLMFQKSFIPKPSSADHFYFGLRAFAVATNSDHCTSHSDDMRILAVS
KLYPLSFSNGLSVFWTNIFQSNLNFSLEIGRPFYSIDPDSFVSFPIKLIS
DKMFSQMNLQCIISENLEIKSVFFDENIFDAQYKIAALPNRFIITILLEN
KGMNYLKSSQWWVDRGLLLFIMYGRITESYMLSRKNDLRTAWASCQIINL
KLQGESKSVAYTSKILQTVHIAINTDKFIIIPILKTRYFFSIFNDINRPI
SLEDDFITLYSIHDVIDGSFVDVTNSAICNSIDKSSIICPLSMMKSSDQY
FHTKFDNKSDNGRTRILPNKFDLILEMKTWKTVLFAPINLWVAQDWKLDI
NVKIINKINGWRVPVRSSVTREIICVTMFQRSRLKVLVRMVNRNTLGEKE
FYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQSDV
NDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDI
YSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFIT
QYDYSSKHYIQVPFVVSRYIPYSNQLNISLYESGSCRFSKLIFTGLVRIN
IQHSEDIPTTISSTMISTEITSTINPSTVNTNQIQNRELIKNLSSLYYGL
YALIALVSLVALIVLVNCLVKFNYRKISHQSSNALNQSVNNKLLNSAQPP
ALADTSSPTTSFTKHPPLLPPPGPLRNASNSEVELPSSTFWSYCSSQDSS
SFTNRKYSSTLLEPTISISPLNNFLIKTSPLVSSVYTNTKYRNNPSPSSD
QGDGGYLSGENQSKITQIPLIKKLSDDRNANLESVDMTSSSSGGLSHYAE
PYPVPPPCPPLKWSIQWATSMVSAIPVFSQSYTPNLITDSTCVISRAVPC
GEPTSPINQWITNKTIPSSSSVPYTKSIDNLCEKSSGLGSLTSTQQEDYS
MLSDDDNYHVSINSDKEHYRKYFKKC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR031437TMEM132_M
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031437Transmembrane protein family 132, middle domainPFAMPF16070TMEM132coord: 498..704
e-value: 1.0E-8
score: 35.0
NoneNo IPR availableTMHMMTMhelixcoord: 798..820