E3 ubiquitin-protein ligase mind-bomb
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035592 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Human
Match: MIB1 (mindbomb E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:21086]) HSP 1 Score: 260.766 bits (665), Expect = 1.286e-74 Identity = 189/631 (29.95%), Postives = 309/631 (48.97%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVK-------------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEVLRLV--DSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------------VNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ + ++G VQ+ D E KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G++++V L+ V VN G + A+ GH D++ LLL++ ++ DKDGD A+H A + + ++L + AD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV A+LD++DK+G+T LH A++ V + ++ + K T +G LG G IA +L NGA + I+N KG++PLD+C D +L + L+ ++ + + PS SN++E +E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAW-EPSKNTLIMG-LGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPNL-CKALAKCHKEKVSGQVGSRS-------PS-MISNDSETLEE------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 56.225 bits (134), Expect = 2.188e-7 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 945 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRI 1003
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 213.772 bits (543), Expect = 4.543e-58 Identity = 168/624 (26.92%), Postives = 296/624 (47.44%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELLVPF--KSVKKHK---IGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKS---------EIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVG--VNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLES--VEALLN-AGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMK-YKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQ-------TESGFGNFLKMTSALGVLGNIGEG-LDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGH-MCCDDCSDNISECHLCSEKILHKIK 1910
D + + Q+ HGGWN M E + TG I D G D V + N E F ++ KH +G V++I D + K LQ HGEW +DM LG+ +V+++F DG+ +++ + + P + + ++ E + D E R +V + GN + L L+ V+T +G L +AA G +L+ LLL+ +A +++ D +G+TALH A N+ + +LL G +A N+ + T +AV +G +++ L D+++ D+ DT LH A+++ S VE L D+ N + F H A++ GH + L R +LV+++KED F A+H+AA+NNH +L++ + D+N++N +L++ LH+AV++ ++ V LV+ ++ +D+ G+TALHVA++ +Q G L++ S L G G L +A +L GA + N++G++PLD+ + R+L ++ +Q+ + P P + L + C +C + L V+F C H C++C+ + +C C + K++
Sbjct: 318 DTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRG-DVRV--------QFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALC-ERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEG----RVLKALQGCAQRFRERQAGGGAAP-GPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 925 HSP 2 Score: 58.9214 bits (141), Expect = 3.279e-8 Identity = 20/57 (35.09%), Postives = 35/57 (61.40%), Query Frame = 3
Query: 1743 ELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
EL+ + ++ME+ C IC D ++++F+CGH C C +S C +C + I +I+I
Sbjct: 947 ELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQI 1003
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 213.772 bits (543), Expect = 5.365e-58 Identity = 168/622 (27.01%), Postives = 295/622 (47.43%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPF--KSVKKHK---IGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKS---------EIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVG--VNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLES--VEALLN-AGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMK-YKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQ-------TESGFGNFLKMTSALGVLGNIGEG-LDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGH-MCCDDCSDNISECHLCSEKILHKIK 1910
D + + Q+ HGGWN M E TG I D G D V + N E F ++ KH +G V++I D + K LQ HGEW +DM LG+ +V+++F DG+ +++ + + P + + ++ E + D E R +V + GN + L L+ V+T +G L +AA G +L+ LLL+ +A +++ D +G+TALH A N+ + +LL G +A N+ + T +AV +G +++ L D+++ D+ DT LH A+++ S VE L D+ N + F H A++ GH + L R +LV+++KED F A+H+AA+NNH +L++ + D+N++N +L++ LH+AV++ ++ V LV+ ++ +D+ G+TALHVA++ +Q G L++ S L G G L +A +L GA + N++G++PLD+ + R+L ++ +Q+ + P P + L + C +C + L V+F C H C++C+ + +C C + K++
Sbjct: 383 DTDVLREMQEGHGGWNPRMAE--TGTVHRITDRG-DVRV--------QFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALC-ERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEG----RVLKALQGCAQRFRERQAGGGAAP-GPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 986 HSP 2 Score: 58.9214 bits (141), Expect = 3.035e-8 Identity = 20/57 (35.09%), Postives = 35/57 (61.40%), Query Frame = 3
Query: 1743 ELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
EL+ + ++ME+ C IC D ++++F+CGH C C +S C +C + I +I+I
Sbjct: 1008 ELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQI 1064
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 213.001 bits (541), Expect = 5.958e-58 Identity = 168/624 (26.92%), Postives = 297/624 (47.60%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELLVPF--KSVKKHK---IGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKS---------EIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVG--VNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLES--VEALLN-AGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMK-YKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQ-------TESGFGNFLKMTSALGVLGNIGEG-LDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGH-MCCDDCSDNISECHLCSEKILHKIK 1910
D + + Q+ HGGWN M E + TG I D G D V + N E F ++ KH +G V++I D + K LQ HGEW +DM LG+ +V+++F DG+ +++ + + P + + ++ E + D E R +V + GN + L L+ V+T +G L +AA G +L+ LLL+ +A +++ D +G+TALH A N+ + +LL G +A N+ + T +AV +G +++ L D+++ D+ DT LH A+++ S VE L D+ N + F H A++ GH + L R +LV+++KED F A+H+AA+NNH +L++ + D+N++N +L++ LH+AV++ ++ V LV+ ++ +D+ G+TALHVA++ +Q G L++ S L G G L +A +L GA + N++G++PLD+ + + R+L ++ +Q+ + P P + L + C +C + L V+F C H C++C+ + +C C + K++
Sbjct: 282 DTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRG-DVRV--------QFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALC-ERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDL----AAEGRVLKALQGCAQRFRERQAGGGAAP-GPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 889 HSP 2 Score: 58.151 bits (139), Expect = 4.527e-8 Identity = 20/57 (35.09%), Postives = 35/57 (61.40%), Query Frame = 3
Query: 1743 ELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
EL+ + ++ME+ C IC D ++++F+CGH C C +S C +C + I +I+I
Sbjct: 911 ELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQI 967
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 213.386 bits (542), Expect = 7.543e-58 Identity = 168/624 (26.92%), Postives = 296/624 (47.44%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELLVPF--KSVKKHK---IGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKS---------EIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVG--VNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLES--VEALLN-AGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMK-YKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQ-------TESGFGNFLKMTSALGVLGNIGEG-LDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGH-MCCDDCSDNISECHLCSEKILHKIK 1910
D + + Q+ HGGWN M E + TG I D G D V + N E F ++ KH +G V++I D + K LQ HGEW +DM LG+ +V+++F DG+ +++ + + P + + ++ E + D E R +V + GN + L L+ V+T +G L +AA G +L+ LLL+ +A +++ D +G+TALH A N+ + +LL G +A N+ + T +AV +G +++ L D+++ D+ DT LH A+++ S VE L D+ N + F H A++ GH + L R +LV+++KED F A+H+AA+NNH +L++ + D+N++N +L++ LH+AV++ ++ V LV+ ++ +D+ G+TALHVA++ +Q G L++ S L G G L +A +L GA + N++G++PLD+ + R+L ++ +Q+ + P P + L + C +C + L V+F C H C++C+ + +C C + K++
Sbjct: 369 DTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRG-DVRV--------QFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALC-ERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEG----RVLKALQGCAQRFRERQAGGGAAP-GPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 976 HSP 2 Score: 58.9214 bits (141), Expect = 3.354e-8 Identity = 20/57 (35.09%), Postives = 35/57 (61.40%), Query Frame = 3
Query: 1743 ELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
EL+ + ++ME+ C IC D ++++F+CGH C C +S C +C + I +I+I
Sbjct: 998 ELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQI 1054
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 125.176 bits (313), Expect = 3.118e-29 Identity = 85/308 (27.60%), Postives = 144/308 (46.75%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICN 1610
T+ G PL IAA+ GHE++ LLLEK AN+N + + LH+A R + +LLL +GA ++++ +TP A G +++LL+ A +S + LH+A +++ LL A + + H+AA GH ++ ++ L R + NS+ + F +H+A N + +L+KY+A + T LH+A ++ V +L++ A D++ G T LH+A + NQT D+ LI NGA +D + + +TPL I +
Sbjct: 223 TSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP--NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQT----------------------------DVVRVLIRNGAKVDAQARELQTPLHIAS 499 HSP 2 Score: 118.627 bits (296), Expect = 3.545e-27 Identity = 93/331 (28.10%), Postives = 154/331 (46.53%), Query Frame = 3
Query: 624 VALKTGNLSEVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
+A + GN V+ L+ + G N+N PL IAA EG E++ +LL+ A+ + K G T LHLA ++ LLL++G V+ + ++TP +A + D + +L+ N A + T LH+A +++E LL AD ++ F HL+A GH++I + ++ + V ++ + A+H+ A +H +L A++N + T LH+A L+ VK LVEN A++ K + T LH A + G N ++ YL+ENGAS + + G+TPL I
Sbjct: 497 IASRLGNTDIVILLLQA-GANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAA-HYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSD--VGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQA-----AQQGHNNCVR-----------------------YLLENGASPNEQTATGQTPLSIA 795 HSP 3 Score: 113.235 bits (282), Expect = 1.756e-25 Identity = 88/307 (28.66%), Postives = 148/307 (48.21%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
T G+ PL +AA G ++V LL++ AN ++ G+T LHLA N+ + +L++ GA V+AQ + TP IA G+ DI+ LL+ + A+ + D + LH+A + E LL+ AD LL KK F HLA+ G+ +++ + L+R V+ + +++ +H+AA N++ +L++ A T LHIA K+ + L++ KA+ + K + G T LH++ + E I+ LIENG+ + K N G T + +C
Sbjct: 421 TTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAG-ANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERG--TPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKE----------------------------ISGLLIENGSDVGAKANNGLTAMHLC 696 HSP 4 Score: 97.8265 bits (242), Expect = 1.186e-20 Identity = 81/306 (26.47%), Postives = 143/306 (46.73%), Query Frame = 3
Query: 603 ERTMRFLVALKTGNLSEVLRLVDS-VGVNT-NLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLIT-SNNADLSIGDS---------------------------DRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK 1430
E + FL A + G+L +VL L+ + +NT N G+ L +A+ EGH ++V L++++A ++ A + G+TALH+A + + +L++ GA+VN Q+ TP +A + +++++ L+ N LS D R ALH+A + LL + + +K F H+AA GHE + ++ L++ VN Q + +H+A + +L+ A ++ + +L T LH A + + V LV A + K KNG LH+A +
Sbjct: 32 EGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGAN--VNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQ 335 HSP 5 Score: 90.8929 bits (224), Expect = 1.598e-18 Identity = 99/357 (27.73%), Postives = 161/357 (45.10%), Query Frame = 3
Query: 594 ITDERTMRFLVALKTGNLS---EVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNF--LKMTSALGVLGNIGEGLDKL-----------EDIAIYLIENGASIDIKNNKGKTPLDIC 1607
I D RT L L S +V+ L+ G + K G+ PL +AA H D LL +A ++ D T LH+A H ++ LLL + AD N++ TP IA K ++ELL+ A + +S T LH+A + V LL GA+ + + HLAA + ++ + + R+G K V++Q + +H+A+ + + +L++ A+ N + + LHIA K L+++ A+ L K G T LH+A K +GN +++ G +I EG +++ + +A+ L+ENGAS G TPL I
Sbjct: 285 IIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGL-TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI-RNGAK-VDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASK-------YGNLEVVRLLLERGTPVDI-EGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIA 630 HSP 6 Score: 66.2402 bits (160), Expect = 7.698e-11 Identity = 55/218 (25.23%), Postives = 104/218 (47.71%), Query Frame = 3
Query: 987 GDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDI-----------AIYLIENGASIDIKNNKGKTPLDIC 1607
G ++ + A + LE V LL AG DI N N+ HLA+ GH +++ +KR + V++ A+H+A++ + +L++ A++N+Q++ T L++A + + VK+L+++ A L ++G T L VA+ + G + + +L N +G +L + A L++N + D+ + G TPL I
Sbjct: 29 GRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQ--VDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVAL-----QQGHDRVVAV-----LLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIA 234
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 125.176 bits (313), Expect = 3.118e-29 Identity = 85/308 (27.60%), Postives = 144/308 (46.75%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICN 1610
T+ G PL IAA+ GHE++ LLLEK AN+N + + LH+A R + +LLL +GA ++++ +TP A G +++LL+ A +S + LH+A +++ LL A + + H+AA GH ++ ++ L R + NS+ + F +H+A N + +L+KY+A + T LH+A ++ V +L++ A D++ G T LH+A + NQT D+ LI NGA +D + + +TPL I +
Sbjct: 223 TSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP--NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQT----------------------------DVVRVLIRNGAKVDAQARELQTPLHIAS 499 HSP 2 Score: 118.627 bits (296), Expect = 3.545e-27 Identity = 93/331 (28.10%), Postives = 154/331 (46.53%), Query Frame = 3
Query: 624 VALKTGNLSEVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
+A + GN V+ L+ + G N+N PL IAA EG E++ +LL+ A+ + K G T LHLA ++ LLL++G V+ + ++TP +A + D + +L+ N A + T LH+A +++E LL AD ++ F HL+A GH++I + ++ + V ++ + A+H+ A +H +L A++N + T LH+A L+ VK LVEN A++ K + T LH A + G N ++ YL+ENGAS + + G+TPL I
Sbjct: 497 IASRLGNTDIVILLLQA-GANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAA-HYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSD--VGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQA-----AQQGHNNCVR-----------------------YLLENGASPNEQTATGQTPLSIA 795 HSP 3 Score: 113.235 bits (282), Expect = 1.756e-25 Identity = 88/307 (28.66%), Postives = 148/307 (48.21%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
T G+ PL +AA G ++V LL++ AN ++ G+T LHLA N+ + +L++ GA V+AQ + TP IA G+ DI+ LL+ + A+ + D + LH+A + E LL+ AD LL KK F HLA+ G+ +++ + L+R V+ + +++ +H+AA N++ +L++ A T LHIA K+ + L++ KA+ + K + G T LH++ + E I+ LIENG+ + K N G T + +C
Sbjct: 421 TTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAG-ANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERG--TPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKE----------------------------ISGLLIENGSDVGAKANNGLTAMHLC 696 HSP 4 Score: 97.8265 bits (242), Expect = 1.186e-20 Identity = 81/306 (26.47%), Postives = 143/306 (46.73%), Query Frame = 3
Query: 603 ERTMRFLVALKTGNLSEVLRLVDS-VGVNT-NLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLIT-SNNADLSIGDS---------------------------DRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK 1430
E + FL A + G+L +VL L+ + +NT N G+ L +A+ EGH ++V L++++A ++ A + G+TALH+A + + +L++ GA+VN Q+ TP +A + +++++ L+ N LS D R ALH+A + LL + + +K F H+AA GHE + ++ L++ VN Q + +H+A + +L+ A ++ + +L T LH A + + V LV A + K KNG LH+A +
Sbjct: 32 EGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGAN--VNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQ 335 HSP 5 Score: 90.8929 bits (224), Expect = 1.598e-18 Identity = 99/357 (27.73%), Postives = 161/357 (45.10%), Query Frame = 3
Query: 594 ITDERTMRFLVALKTGNLS---EVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNF--LKMTSALGVLGNIGEGLDKL-----------EDIAIYLIENGASIDIKNNKGKTPLDIC 1607
I D RT L L S +V+ L+ G + K G+ PL +AA H D LL +A ++ D T LH+A H ++ LLL + AD N++ TP IA K ++ELL+ A + +S T LH+A + V LL GA+ + + HLAA + ++ + + R+G K V++Q + +H+A+ + + +L++ A+ N + + LHIA K L+++ A+ L K G T LH+A K +GN +++ G +I EG +++ + +A+ L+ENGAS G TPL I
Sbjct: 285 IIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGL-TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI-RNGAK-VDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASK-------YGNLEVVRLLLERGTPVDI-EGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIA 630 HSP 6 Score: 66.2402 bits (160), Expect = 7.698e-11 Identity = 55/218 (25.23%), Postives = 104/218 (47.71%), Query Frame = 3
Query: 987 GDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDI-----------AIYLIENGASIDIKNNKGKTPLDIC 1607
G ++ + A + LE V LL AG DI N N+ HLA+ GH +++ +KR + V++ A+H+A++ + +L++ A++N+Q++ T L++A + + VK+L+++ A L ++G T L VA+ + G + + +L N +G +L + A L++N + D+ + G TPL I
Sbjct: 29 GRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQ--VDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVAL-----QQGHDRVVAV-----LLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIA 234
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 125.176 bits (313), Expect = 3.242e-29 Identity = 85/308 (27.60%), Postives = 144/308 (46.75%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICN 1610
T+ G PL IAA+ GHE++ LLLEK AN+N + + LH+A R + +LLL +GA ++++ +TP A G +++LL+ A +S + LH+A +++ LL A + + H+AA GH ++ ++ L R + NS+ + F +H+A N + +L+KY+A + T LH+A ++ V +L++ A D++ G T LH+A + NQT D+ LI NGA +D + + +TPL I +
Sbjct: 223 TSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP--NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQT----------------------------DVVRVLIRNGAKVDAQARELQTPLHIAS 499 HSP 2 Score: 118.627 bits (296), Expect = 3.952e-27 Identity = 93/331 (28.10%), Postives = 154/331 (46.53%), Query Frame = 3
Query: 624 VALKTGNLSEVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
+A + GN V+ L+ + G N+N PL IAA EG E++ +LL+ A+ + K G T LHLA ++ LLL++G V+ + ++TP +A + D + +L+ N A + T LH+A +++E LL AD ++ F HL+A GH++I + ++ + V ++ + A+H+ A +H +L A++N + T LH+A L+ VK LVEN A++ K + T LH A + G N ++ YL+ENGAS + + G+TPL I
Sbjct: 497 IASRLGNTDIVILLLQA-GANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAA-HYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSD--VGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQA-----AQQGHNNCVR-----------------------YLLENGASPNEQTATGQTPLSIA 795 HSP 3 Score: 113.235 bits (282), Expect = 2.117e-25 Identity = 88/307 (28.66%), Postives = 148/307 (48.21%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
T G+ PL +AA G ++V LL++ AN ++ G+T LHLA N+ + +L++ GA V+AQ + TP IA G+ DI+ LL+ + A+ + D + LH+A + E LL+ AD LL KK F HLA+ G+ +++ + L+R V+ + +++ +H+AA N++ +L++ A T LHIA K+ + L++ KA+ + K + G T LH++ + E I+ LIENG+ + K N G T + +C
Sbjct: 421 TTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAG-ANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTP--VDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKE----------------------------ISGLLIENGSDVGAKANNGLTAMHLC 696 HSP 4 Score: 97.4413 bits (241), Expect = 1.465e-20 Identity = 81/306 (26.47%), Postives = 143/306 (46.73%), Query Frame = 3
Query: 603 ERTMRFLVALKTGNLSEVLRLVDS-VGVNT-NLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSN-NADLSIGDS---------------------------DRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK 1430
E + FL A + G+L +VL L+ + +NT N G+ L +A+ EGH ++V L++++A ++ A + G+TALH+A + + +L++ GA+VN Q+ TP +A + +++++ L+ N LS D R ALH+A + LL + + +K F H+AA GHE + ++ L++ VN Q + +H+A + +L+ A ++ + +L T LH A + + V LV A + K KNG LH+A +
Sbjct: 32 EGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGAN--VNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQ 335 HSP 5 Score: 90.8929 bits (224), Expect = 1.551e-18 Identity = 99/357 (27.73%), Postives = 161/357 (45.10%), Query Frame = 3
Query: 594 ITDERTMRFLVALKTGNLS---EVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNF--LKMTSALGVLGNIGEGLDKL-----------EDIAIYLIENGASIDIKNNKGKTPLDIC 1607
I D RT L L S +V+ L+ G + K G+ PL +AA H D LL +A ++ D T LH+A H ++ LLL + AD N++ TP IA K ++ELL+ A + +S T LH+A + V LL GA+ + + HLAA + ++ + + R+G K V++Q + +H+A+ + + +L++ A+ N + + LHIA K L+++ A+ L K G T LH+A K +GN +++ G +I EG +++ + +A+ L+ENGAS G TPL I
Sbjct: 285 IIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGL-TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI-RNGAK-VDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASK-------YGNLEVVRLLLERGTPVDI-EGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIA 630 HSP 6 Score: 65.855 bits (159), Expect = 8.580e-11 Identity = 55/218 (25.23%), Postives = 104/218 (47.71%), Query Frame = 3
Query: 987 GDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDI-----------AIYLIENGASIDIKNNKGKTPLDIC 1607
G ++ + A + LE V LL AG DI N N+ HLA+ GH +++ +KR + V++ A+H+A++ + +L++ A++N+Q++ T L++A + + VK+L+++ A L ++G T L VA+ + G + + +L N +G +L + A L++N + D+ + G TPL I
Sbjct: 29 GRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQ--VDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVAL-----QQGHDRVVAV-----LLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIA 234
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 125.176 bits (313), Expect = 3.242e-29 Identity = 85/308 (27.60%), Postives = 144/308 (46.75%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICN 1610
T+ G PL IAA+ GHE++ LLLEK AN+N + + LH+A R + +LLL +GA ++++ +TP A G +++LL+ A +S + LH+A +++ LL A + + H+AA GH ++ ++ L R + NS+ + F +H+A N + +L+KY+A + T LH+A ++ V +L++ A D++ G T LH+A + NQT D+ LI NGA +D + + +TPL I +
Sbjct: 223 TSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP--NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQT----------------------------DVVRVLIRNGAKVDAQARELQTPLHIAS 499 HSP 2 Score: 118.627 bits (296), Expect = 3.952e-27 Identity = 93/331 (28.10%), Postives = 154/331 (46.53%), Query Frame = 3
Query: 624 VALKTGNLSEVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
+A + GN V+ L+ + G N+N PL IAA EG E++ +LL+ A+ + K G T LHLA ++ LLL++G V+ + ++TP +A + D + +L+ N A + T LH+A +++E LL AD ++ F HL+A GH++I + ++ + V ++ + A+H+ A +H +L A++N + T LH+A L+ VK LVEN A++ K + T LH A + G N ++ YL+ENGAS + + G+TPL I
Sbjct: 497 IASRLGNTDIVILLLQA-GANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAA-HYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSD--VGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQA-----AQQGHNNCVR-----------------------YLLENGASPNEQTATGQTPLSIA 795 HSP 3 Score: 113.235 bits (282), Expect = 2.117e-25 Identity = 88/307 (28.66%), Postives = 148/307 (48.21%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
T G+ PL +AA G ++V LL++ AN ++ G+T LHLA N+ + +L++ GA V+AQ + TP IA G+ DI+ LL+ + A+ + D + LH+A + E LL+ AD LL KK F HLA+ G+ +++ + L+R V+ + +++ +H+AA N++ +L++ A T LHIA K+ + L++ KA+ + K + G T LH++ + E I+ LIENG+ + K N G T + +C
Sbjct: 421 TTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAG-ANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTP--VDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKE----------------------------ISGLLIENGSDVGAKANNGLTAMHLC 696 HSP 4 Score: 97.4413 bits (241), Expect = 1.465e-20 Identity = 81/306 (26.47%), Postives = 143/306 (46.73%), Query Frame = 3
Query: 603 ERTMRFLVALKTGNLSEVLRLVDS-VGVNT-NLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSN-NADLSIGDS---------------------------DRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK 1430
E + FL A + G+L +VL L+ + +NT N G+ L +A+ EGH ++V L++++A ++ A + G+TALH+A + + +L++ GA+VN Q+ TP +A + +++++ L+ N LS D R ALH+A + LL + + +K F H+AA GHE + ++ L++ VN Q + +H+A + +L+ A ++ + +L T LH A + + V LV A + K KNG LH+A +
Sbjct: 32 EGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGAN--VNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQ 335 HSP 5 Score: 90.8929 bits (224), Expect = 1.551e-18 Identity = 99/357 (27.73%), Postives = 161/357 (45.10%), Query Frame = 3
Query: 594 ITDERTMRFLVALKTGNLS---EVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNF--LKMTSALGVLGNIGEGLDKL-----------EDIAIYLIENGASIDIKNNKGKTPLDIC 1607
I D RT L L S +V+ L+ G + K G+ PL +AA H D LL +A ++ D T LH+A H ++ LLL + AD N++ TP IA K ++ELL+ A + +S T LH+A + V LL GA+ + + HLAA + ++ + + R+G K V++Q + +H+A+ + + +L++ A+ N + + LHIA K L+++ A+ L K G T LH+A K +GN +++ G +I EG +++ + +A+ L+ENGAS G TPL I
Sbjct: 285 IIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGL-TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI-RNGAK-VDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASK-------YGNLEVVRLLLERGTPVDI-EGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIA 630 HSP 6 Score: 65.855 bits (159), Expect = 8.580e-11 Identity = 55/218 (25.23%), Postives = 104/218 (47.71%), Query Frame = 3
Query: 987 GDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDI-----------AIYLIENGASIDIKNNKGKTPLDIC 1607
G ++ + A + LE V LL AG DI N N+ HLA+ GH +++ +KR + V++ A+H+A++ + +L++ A++N+Q++ T L++A + + VK+L+++ A L ++G T L VA+ + G + + +L N +G +L + A L++N + D+ + G TPL I
Sbjct: 29 GRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQ--VDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVAL-----QQGHDRVVAV-----LLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIA 234
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 125.176 bits (313), Expect = 3.512e-29 Identity = 85/308 (27.60%), Postives = 144/308 (46.75%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICN 1610
T+ G PL IAA+ GHE++ LLLEK AN+N + + LH+A R + +LLL +GA ++++ +TP A G +++LL+ A +S + LH+A +++ LL A + + H+AA GH ++ ++ L R + NS+ + F +H+A N + +L+KY+A + T LH+A ++ V +L++ A D++ G T LH+A + NQT D+ LI NGA +D + + +TPL I +
Sbjct: 223 TSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP--NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQT----------------------------DVVRVLIRNGAKVDAQARELQTPLHIAS 499 HSP 2 Score: 118.627 bits (296), Expect = 3.853e-27 Identity = 93/331 (28.10%), Postives = 154/331 (46.53%), Query Frame = 3
Query: 624 VALKTGNLSEVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
+A + GN V+ L+ + G N+N PL IAA EG E++ +LL+ A+ + K G T LHLA ++ LLL++G V+ + ++TP +A + D + +L+ N A + T LH+A +++E LL AD ++ F HL+A GH++I + ++ + V ++ + A+H+ A +H +L A++N + T LH+A L+ VK LVEN A++ K + T LH A + G N ++ YL+ENGAS + + G+TPL I
Sbjct: 497 IASRLGNTDIVILLLQA-GANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAA-HYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSD--VGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQA-----AQQGHNNCVR-----------------------YLLENGASPNEQTATGQTPLSIA 795 HSP 3 Score: 113.235 bits (282), Expect = 1.891e-25 Identity = 88/307 (28.66%), Postives = 148/307 (48.21%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
T G+ PL +AA G ++V LL++ AN ++ G+T LHLA N+ + +L++ GA V+AQ + TP IA G+ DI+ LL+ + A+ + D + LH+A + E LL+ AD LL KK F HLA+ G+ +++ + L+R V+ + +++ +H+AA N++ +L++ A T LHIA K+ + L++ KA+ + K + G T LH++ + E I+ LIENG+ + K N G T + +C
Sbjct: 421 TTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAG-ANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERG--TPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKE----------------------------ISGLLIENGSDVGAKANNGLTAMHLC 696 HSP 4 Score: 97.8265 bits (242), Expect = 1.378e-20 Identity = 81/306 (26.47%), Postives = 143/306 (46.73%), Query Frame = 3
Query: 603 ERTMRFLVALKTGNLSEVLRLVDS-VGVNT-NLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLIT-SNNADLSIGDS---------------------------DRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK 1430
E + FL A + G+L +VL L+ + +NT N G+ L +A+ EGH ++V L++++A ++ A + G+TALH+A + + +L++ GA+VN Q+ TP +A + +++++ L+ N LS D R ALH+A + LL + + +K F H+AA GHE + ++ L++ VN Q + +H+A + +L+ A ++ + +L T LH A + + V LV A + K KNG LH+A +
Sbjct: 32 EGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGAN--VNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQ 335 HSP 5 Score: 90.8929 bits (224), Expect = 1.732e-18 Identity = 99/357 (27.73%), Postives = 161/357 (45.10%), Query Frame = 3
Query: 594 ITDERTMRFLVALKTGNLS---EVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNF--LKMTSALGVLGNIGEGLDKL-----------EDIAIYLIENGASIDIKNNKGKTPLDIC 1607
I D RT L L S +V+ L+ G + K G+ PL +AA H D LL +A ++ D T LH+A H ++ LLL + AD N++ TP IA K ++ELL+ A + +S T LH+A + V LL GA+ + + HLAA + ++ + + R+G K V++Q + +H+A+ + + +L++ A+ N + + LHIA K L+++ A+ L K G T LH+A K +GN +++ G +I EG +++ + +A+ L+ENGAS G TPL I
Sbjct: 285 IIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGL-TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI-RNGAK-VDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASK-------YGNLEVVRLLLERGTPVDI-EGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIA 630 HSP 6 Score: 65.855 bits (159), Expect = 8.694e-11 Identity = 55/218 (25.23%), Postives = 104/218 (47.71%), Query Frame = 3
Query: 987 GDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDI-----------AIYLIENGASIDIKNNKGKTPLDIC 1607
G ++ + A + LE V LL AG DI N N+ HLA+ GH +++ +KR + V++ A+H+A++ + +L++ A++N+Q++ T L++A + + VK+L+++ A L ++G T L VA+ + G + + +L N +G +L + A L++N + D+ + G TPL I
Sbjct: 29 GRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQ--VDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVAL-----QQGHDRVVAV-----LLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIA 234
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Fly
Match: mib1 (gene:FBgn0263601 transcript:FBtr0333578) HSP 1 Score: 254.988 bits (650), Expect = 2.144e-72 Identity = 203/691 (29.38%), Postives = 327/691 (47.32%), Query Frame = 3
Query: 81 GANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLV----PFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHP----KTIKSEIVENTELKXXXXXXXXXXXXXXL----ITDERTMRFLVALKTGNLSEVLRLVDS----------------VGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFL-KRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVN--------QTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSL-KTRILSLVER----------------------------DSQKHHITKKELLEHPVQPSDARSNEAEL----MELRQKLKEMEDEKI-----CKICHDRRLKVIFE-CGHM-CCDDCSDNISECHLCSEKILHKIKIN 1916
G F I KV + + DL + Q HGGW D M ECL+ ++ D+ D V SG + N +L P + + ++G V++ D E K+LQ HGEW + M+ LGK V Q++ D D K+ N + +P K S + + S + + + L ++ + T F+ A G + + V VN G L A+ GH +++ +LL ++ I DKDGD A+H A + + ++L K GAD+NA+N ++ T IAV KG ++++ L+T S+ DS+ DT LH A++ E + LL+ GADI L N FNA H AA+ G+ ++I L K + +V +K+D + A+H+AA+NNH +L+ KA+++ QN+ L+TALH+AV+R+++ VK LV++ A+L++ DK+G+T LH A++ + Q GFG L +G+ IA +L NGA + +KN K +TPLD+C D +L KT + ER S ++ L P+ + S+ L E+ Q L E + C +C D + +F+ CGH+ CC+ C+ + +C +C E + + KI+
Sbjct: 223 GKGPHGFQIGDKVTV-DLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSSERLSAILKKLFEPNVSGDATEEFVKAAANGFAARCEEYLAGAAQPSTSSASPSSGPDVNVNGVFAGHTALQAASQNGHIEVIQVLLRHAVDVEIEDKDGDRAVHHAAFGDEAAVIEILAKAGADLNARNKRRQTSLHIAVNKGHLNVVKTLLTL-GCHPSLQDSEGDTPLHDAISKEHDEMLSLLLDFGADITLNNNNGFNALHHAALKGNPSAMKILLTKTNRPWIVEEKKDDGYTALHLAALNNHVEIAELLVHMGKANMDRQNVNLQTALHLAVERQHVQIVKLLVQDGADLNIPDKDGDTPLHEALRHHTLSQLKQLQDVEGFGKLL-----MGLRN--ANNKKASASIACFLAANGADLTLKNRKQQTPLDLCPDPNLCKTLVKCYNERKTDDSELPGNVAGTSSSARARAASGSLNQSSSVNMPLSSLAASSTFPAASSSSIFALNGIANEMSQSLHEDPPKSSASLDECLVCSDAKRDTVFKPCGHVSCCETCAPRVKKCLICRETVSSREKID 904 HSP 2 Score: 52.373 bits (124), Expect = 1.610e-6 Identity = 21/70 (30.00%), Postives = 43/70 (61.43%), Query Frame = 3
Query: 1701 VQPSDARSNEAE-LMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
V PS+ + + + + +L+Q+L++++++ +C +C DR ++F CGH C C D I C +C + + +I
Sbjct: 1043 VAPSNVNNFQMDDVQKLKQQLQDIKEQTMCPVCFDRIKNMVFLCGHGTCQMCGDQIEGCPICRKTVEKRI 1112
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Fly
Match: mib1 (gene:FBgn0263601 transcript:FBtr0075520) HSP 1 Score: 254.603 bits (649), Expect = 4.372e-72 Identity = 203/691 (29.38%), Postives = 327/691 (47.32%), Query Frame = 3
Query: 81 GANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLV----PFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHP----KTIKSEIVENTELKXXXXXXXXXXXXXXL----ITDERTMRFLVALKTGNLSEVLRLVDS----------------VGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFL-KRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVN--------QTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSL-KTRILSLVER----------------------------DSQKHHITKKELLEHPVQPSDARSNEAEL----MELRQKLKEMEDEKI-----CKICHDRRLKVIFE-CGHM-CCDDCSDNISECHLCSEKILHKIKIN 1916
G F I KV + + DL + Q HGGW D M ECL+ ++ D+ D V SG + N +L P + + ++G V++ D E K+LQ HGEW + M+ LGK V Q++ D D K+ N + +P K S + + S + + + L ++ + T F+ A G + + V VN G L A+ GH +++ +LL ++ I DKDGD A+H A + + ++L K GAD+NA+N ++ T IAV KG ++++ L+T S+ DS+ DT LH A++ E + LL+ GADI L N FNA H AA+ G+ ++I L K + +V +K+D + A+H+AA+NNH +L+ KA+++ QN+ L+TALH+AV+R+++ VK LV++ A+L++ DK+G+T LH A++ + Q GFG L +G+ IA +L NGA + +KN K +TPLD+C D +L KT + ER S ++ L P+ + S+ L E+ Q L E + C +C D + +F+ CGH+ CC+ C+ + +C +C E + + KI+
Sbjct: 334 GKGPHGFQIGDKVTV-DLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSSERLSAILKKLFEPNVSGDATEEFVKAAANGFAARCEEYLAGAAQPSTSSASPSSGPDVNVNGVFAGHTALQAASQNGHIEVIQVLLRHAVDVEIEDKDGDRAVHHAAFGDEAAVIEILAKAGADLNARNKRRQTSLHIAVNKGHLNVVKTLLTL-GCHPSLQDSEGDTPLHDAISKEHDEMLSLLLDFGADITLNNNNGFNALHHAALKGNPSAMKILLTKTNRPWIVEEKKDDGYTALHLAALNNHVEIAELLVHMGKANMDRQNVNLQTALHLAVERQHVQIVKLLVQDGADLNIPDKDGDTPLHEALRHHTLSQLKQLQDVEGFGKLL-----MGLRN--ANNKKASASIACFLAANGADLTLKNRKQQTPLDLCPDPNLCKTLVKCYNERKTDDSELPGNVAGTSSSARARAASGSLNQSSSVNMPLSSLAASSTFPAASSSSIFALNGIANEMSQSLHEDPPKSSASLDECLVCSDAKRDTVFKPCGHVSCCETCAPRVKKCLICRETVSSREKID 1015 HSP 2 Score: 52.373 bits (124), Expect = 1.613e-6 Identity = 21/70 (30.00%), Postives = 43/70 (61.43%), Query Frame = 3
Query: 1701 VQPSDARSNEAE-LMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
V PS+ + + + + +L+Q+L++++++ +C +C DR ++F CGH C C D I C +C + + +I
Sbjct: 1154 VAPSNVNNFQMDDVQKLKQQLQDIKEQTMCPVCFDRIKNMVFLCGHGTCQMCGDQIEGCPICRKTVEKRI 1223
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Fly
Match: mib2 (gene:FBgn0086442 transcript:FBtr0336840) HSP 1 Score: 177.178 bits (448), Expect = 4.329e-46 Identity = 142/470 (30.21%), Postives = 238/470 (50.64%), Query Frame = 3
Query: 93 SSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLT--GPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIF--IVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALK-------------TGNLSEVLRLVD--SVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNA-GADIYLLNKKLFNAYHLAAISGH----EKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM 1427
SF + +V + ++ ++K Q+ HGGWN M E L+ G I D G + + LV V ++G V +I D + + LQ HGEW E MR+ LGK +V++++ DGD +I ++ F ++PK +K +E + L + + + D M L L G+L V + +D V+ G + +A+++G+ DLV+ L+ K AN+N DK+GD+ALH A N+ +LL+ GA+VN N+ + I K + + L+ +NA+++I DS DTALH A+ E VE L NA D + N + FN H AA+ G+ +IL L R +LVN +K+D FAA+H+AA+N H + L+ + +A+L+++N +T +AV + + ++ LV +++ KD++G+ A+H+ +
Sbjct: 251 PSFSVGDRVKVC-LEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALV---KVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVK---LERSPLATAAERSNSMMDLSHRRADH-VMTPLSGLSGSSVADKLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELL-QHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRIL--LLSR---QLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCV 706 HSP 2 Score: 60.8474 bits (146), Expect = 3.502e-9 Identity = 55/203 (27.09%), Postives = 94/203 (46.31%), Query Frame = 3
Query: 639 GNLSEVLRLVDSVGVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLL-KKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAG-ADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAM 1232
GN +R++ G N L I A++ V LL+ AN+NI D GDTALH A+ + ++ +LL D +NN+ A LKG+ ++ + +++ D ALHLA + + VE L+ G A++ + N + + LA GH ++E ++ + VN++ ED A+H+ +
Sbjct: 507 GNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCD--VNAKDEDGDNAMHLCVI 707 HSP 3 Score: 56.9954 bits (136), Expect = 4.829e-8 Identity = 56/244 (22.95%), Postives = 106/244 (43.44%), Query Frame = 3
Query: 927 KGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNG---NTALHVAMKVN-----------------QTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPL 1598
+G D + + N + + + + + +A + ++ V L++ GA++ ++K+ +A H AA + + L+ E VN +A+H+ A HC+ L+++ A++N+Q+ TALH A+ ++ V+ L+ N LD KN N H A+K N + + GF L L + ++ L + E A +DI+NN+ +TP
Sbjct: 441 QGHLDFVRQYLDVNPSQVDVMSGGK-ACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAE--VNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVE-LLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAAL-----HLAALNGHAQVVETL------VTEGQAELDIRNNRQQTPF 669 HSP 4 Score: 56.6102 bits (135), Expect = 7.466e-8 Identity = 27/71 (38.03%), Postives = 39/71 (54.93%), Query Frame = 3
Query: 1707 PSDA-RSNEAELME-LRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
PSD R +L+ L K+ E E+ C IC +R+ V F CGH C C++ + CH+C + IL KI +
Sbjct: 978 PSDPTRLPSGDLLRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINL 1048
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Fly
Match: mib2 (gene:FBgn0086442 transcript:FBtr0081142) HSP 1 Score: 177.178 bits (448), Expect = 4.329e-46 Identity = 142/470 (30.21%), Postives = 238/470 (50.64%), Query Frame = 3
Query: 93 SSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLT--GPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIF--IVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALK-------------TGNLSEVLRLVD--SVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNA-GADIYLLNKKLFNAYHLAAISGH----EKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM 1427
SF + +V + ++ ++K Q+ HGGWN M E L+ G I D G + + LV V ++G V +I D + + LQ HGEW E MR+ LGK +V++++ DGD +I ++ F ++PK +K +E + L + + + D M L L G+L V + +D V+ G + +A+++G+ DLV+ L+ K AN+N DK+GD+ALH A N+ +LL+ GA+VN N+ + I K + + L+ +NA+++I DS DTALH A+ E VE L NA D + N + FN H AA+ G+ +IL L R +LVN +K+D FAA+H+AA+N H + L+ + +A+L+++N +T +AV + + ++ LV +++ KD++G+ A+H+ +
Sbjct: 251 PSFSVGDRVKVC-LEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALV---KVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVK---LERSPLATAAERSNSMMDLSHRRADH-VMTPLSGLSGSSVADKLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELL-QHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRIL--LLSR---QLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCV 706 HSP 2 Score: 60.8474 bits (146), Expect = 3.502e-9 Identity = 55/203 (27.09%), Postives = 94/203 (46.31%), Query Frame = 3
Query: 639 GNLSEVLRLVDSVGVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLL-KKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAG-ADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAM 1232
GN +R++ G N L I A++ V LL+ AN+NI D GDTALH A+ + ++ +LL D +NN+ A LKG+ ++ + +++ D ALHLA + + VE L+ G A++ + N + + LA GH ++E ++ + VN++ ED A+H+ +
Sbjct: 507 GNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCD--VNAKDEDGDNAMHLCVI 707 HSP 3 Score: 56.9954 bits (136), Expect = 4.829e-8 Identity = 56/244 (22.95%), Postives = 106/244 (43.44%), Query Frame = 3
Query: 927 KGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNG---NTALHVAMKVN-----------------QTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPL 1598
+G D + + N + + + + + +A + ++ V L++ GA++ ++K+ +A H AA + + L+ E VN +A+H+ A HC+ L+++ A++N+Q+ TALH A+ ++ V+ L+ N LD KN N H A+K N + + GF L L + ++ L + E A +DI+NN+ +TP
Sbjct: 441 QGHLDFVRQYLDVNPSQVDVMSGGK-ACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAE--VNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVE-LLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAAL-----HLAALNGHAQVVETL------VTEGQAELDIRNNRQQTPF 669 HSP 4 Score: 56.6102 bits (135), Expect = 7.466e-8 Identity = 27/71 (38.03%), Postives = 39/71 (54.93%), Query Frame = 3
Query: 1707 PSDA-RSNEAELME-LRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
PSD R +L+ L K+ E E+ C IC +R+ V F CGH C C++ + CH+C + IL KI +
Sbjct: 978 PSDPTRLPSGDLLRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINL 1048
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Fly
Match: mib2 (gene:FBgn0086442 transcript:FBtr0336841) HSP 1 Score: 177.178 bits (448), Expect = 4.329e-46 Identity = 142/470 (30.21%), Postives = 238/470 (50.64%), Query Frame = 3
Query: 93 SSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLT--GPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIF--IVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALK-------------TGNLSEVLRLVD--SVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNA-GADIYLLNKKLFNAYHLAAISGH----EKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM 1427
SF + +V + ++ ++K Q+ HGGWN M E L+ G I D G + + LV V ++G V +I D + + LQ HGEW E MR+ LGK +V++++ DGD +I ++ F ++PK +K +E + L + + + D M L L G+L V + +D V+ G + +A+++G+ DLV+ L+ K AN+N DK+GD+ALH A N+ +LL+ GA+VN N+ + I K + + L+ +NA+++I DS DTALH A+ E VE L NA D + N + FN H AA+ G+ +IL L R +LVN +K+D FAA+H+AA+N H + L+ + +A+L+++N +T +AV + + ++ LV +++ KD++G+ A+H+ +
Sbjct: 251 PSFSVGDRVKVC-LEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALV---KVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVK---LERSPLATAAERSNSMMDLSHRRADH-VMTPLSGLSGSSVADKLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELL-QHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRIL--LLSR---QLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCV 706 HSP 2 Score: 60.8474 bits (146), Expect = 3.502e-9 Identity = 55/203 (27.09%), Postives = 94/203 (46.31%), Query Frame = 3
Query: 639 GNLSEVLRLVDSVGVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLL-KKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAG-ADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAM 1232
GN +R++ G N L I A++ V LL+ AN+NI D GDTALH A+ + ++ +LL D +NN+ A LKG+ ++ + +++ D ALHLA + + VE L+ G A++ + N + + LA GH ++E ++ + VN++ ED A+H+ +
Sbjct: 507 GNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCD--VNAKDEDGDNAMHLCVI 707 HSP 3 Score: 56.9954 bits (136), Expect = 4.829e-8 Identity = 56/244 (22.95%), Postives = 106/244 (43.44%), Query Frame = 3
Query: 927 KGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNG---NTALHVAMKVN-----------------QTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPL 1598
+G D + + N + + + + + +A + ++ V L++ GA++ ++K+ +A H AA + + L+ E VN +A+H+ A HC+ L+++ A++N+Q+ TALH A+ ++ V+ L+ N LD KN N H A+K N + + GF L L + ++ L + E A +DI+NN+ +TP
Sbjct: 441 QGHLDFVRQYLDVNPSQVDVMSGGK-ACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAE--VNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVE-LLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAAL-----HLAALNGHAQVVETL------VTEGQAELDIRNNRQQTPF 669 HSP 4 Score: 56.6102 bits (135), Expect = 7.466e-8 Identity = 27/71 (38.03%), Postives = 39/71 (54.93%), Query Frame = 3
Query: 1707 PSDA-RSNEAELME-LRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
PSD R +L+ L K+ E E+ C IC +R+ V F CGH C C++ + CH+C + IL KI +
Sbjct: 978 PSDPTRLPSGDLLRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINL 1048
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Zebrafish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 260.381 bits (664), Expect = 1.707e-74 Identity = 192/630 (30.48%), Postives = 305/630 (48.41%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKI-------------GSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEV---LRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGV-------------LGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ ++ G VQ+ D + KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G+L++V L+ D V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A++ + T S M V LG G IA +L NGA + I+N KG++PLD+C D SL + L+ ++ + + PS +NE L+E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPD-VDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALR-HHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSL-CKALAKCHKEKTSGQVGSRS-------PSLNSNNET--------LEE------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 861 HSP 2 Score: 56.6102 bits (135), Expect = 8.679e-8 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 969 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRI 1027
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Zebrafish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 258.84 bits (660), Expect = 6.261e-74 Identity = 192/637 (30.14%), Postives = 313/637 (49.14%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKI-------------GSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEV---LRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------------VNQTESG--FGNFLKMTSALGVLGNIG-EGLDK--LEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ ++ G VQ+ D + KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G+L++V L+ D V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A++ V++ E N ++ L ++ +G +G +K IA +L NGA + I+N KG++PLD+C D SL + L+ ++ + + PS +NE L+E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPD-VDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTVRTEKCLKLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSL-CKALAKCHKEKTSGQVGSRS-------PSLNSNNET--------LEE------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 869 HSP 2 Score: 56.6102 bits (135), Expect = 9.473e-8 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 977 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRI 1035
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Zebrafish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 256.144 bits (653), Expect = 6.138e-73 Identity = 193/644 (29.97%), Postives = 309/644 (47.98%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKI-------------GSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVAL----KTGNLSE----------------VLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGV-------------LGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ ++ G VQ+ D + KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + +S + ER + L L ++G+++E +L+ D V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A++ + T S M V LG G IA +L NGA + I+N KG++PLD+C D SL + L+ ++ + + PS +NE L+E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPS---VPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPD-VDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALR-HHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSL-CKALAKCHKEKTSGQVGSRS-------PSLNSNNET--------LEE------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 872 HSP 2 Score: 56.9954 bits (136), Expect = 7.856e-8 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 980 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRI 1038
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Zebrafish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:ZFIN;Acc:ZDB-GENE-050628-1]) HSP 1 Score: 153.68 bits (387), Expect = 2.181e-38 Identity = 159/618 (25.73%), Postives = 284/618 (45.95%), Query Frame = 3
Query: 159 QKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSE---------IVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLK--GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEAL-LNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQ------TESGFGNFL--KMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQ-----------KLKEMEDEKICKICHDRRLKVIFE-CGH-MCCDDCSDNISECHLCSEKILHKIK 1910
Q+ HGGWN M E G LI D G D V + + + + L K+ + H + + G E + LG+ +V++++ DGD +++ + + +P + ++ VEN S + + R ++ G+ ++V L+ ++K G L +AA++GH ++V +LL+ ++I D+DGD ALH F + D + + Q+ K + +A + ++ I+ + + + DS DT LH A+ +E L + D N + FN H AA+ G++ E+ L R +L + +KED F+A+H+AA+NNH +L+K + D+N++N +T L +AV + +++ V LV A+++ +D++G+TA+H ++ Q + G G L ++ S+ G++GN L+ IA +L + GA I N+KGK+PLD+ D+S++T I S E KH + H V S + + LR+ + + C IC + L V+F C H + C++C+ + +C C I KI+
Sbjct: 220 QEGHGGWNPKMAEIRDG--ALITDRG-DVRVQYSNIIRWTFHPGALT--KARRTHTHTHTHTHTHTHQSYLAQVCLIGVGAELL--ALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPGEVDANLMTVENASESSSTVISVLEKLLSQSTEQDNPGRLVIEAAHGSAAKVRELLQKHPDKVDIKNQGKTALQVAAHQGHVEVVKVLLQANSSIEAKDEDGDAALHYTA----FGIADSRVITLNKAHYQHLKGLCSLHMAENR-TEHIVFQCVHVCVCVVCVQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILLKEGRCDINIRNNRNQTPLQLAVTQGHMALVALLVMEGADVNAEDEDGDTAMHTSLSRQQLTTVISSAEGDGAVLYGRLCSS-GLMGNTE--LNVGAAIACFLAQEGADISYANHKGKSPLDLVTDSSVQTLIRSFAE----KHRL-------HAV--SCGTGSSSSSSSLRRVHTTPNTMTNLAVPSVTGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIRCQVTITKKIR 808 HSP 2 Score: 60.4622 bits (145), Expect = 5.228e-9 Identity = 29/85 (34.12%), Postives = 48/85 (56.47%), Query Frame = 3
Query: 1671 ITKK---ELLEHPVQPSDARSNEAELME-LRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
ITKK + E PS S + L+E L+ + ++ME+ C IC D ++++F+CGH C DCS + C +C + I +I++
Sbjct: 803 ITKKIRQDQTEVECSPSSENSEQHHLLEQLQSRYRQMEERITCPICIDNHIRLVFQCGHASCIDCSSALKTCPICRQTIRERIQL 887
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Zebrafish
Match: ripk4 (receptor-interacting serine-threonine kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040426-2042]) HSP 1 Score: 130.183 bits (326), Expect = 7.037e-31 Identity = 82/264 (31.06%), Postives = 138/264 (52.27%), Query Frame = 3
Query: 642 NLSEVLRLVDSVGVNTNLKGMY-PLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAEL-DLKDKNGNTALHVAM 1427
+SE+L + VN + Y PL AA G E L LLL++ A+IN D G T H+A H + + +LL +GADV+ + T +A KG I++LL+ AD+ SD + LHLA + L+ GA+++L + L+ H+AA +GH + +K D + + S+ + A+H+A+ H + +L+ AD N +LRT H+A + + +K L+ + +++ + +D+NG TALH+A+
Sbjct: 496 GVSEILLSRKTTNVNAKDEDQYTPLHFAAQNGDEALTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVLLSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADVDGQTSDGRSPLHLASQRGQYRVARILVELGANVHLTSDDLYAPLHVAAETGHTSTSRLLVKHDAD--IKSRTANGCTALHLASQKGHLPTVKMLLAEGADPESVNHDLRTPCHLAAQNGHCEVLKELLRSCSDVANAQDRNGLTALHLAV 757 HSP 2 Score: 101.293 bits (251), Expect = 1.156e-21 Identity = 77/255 (30.20%), Postives = 127/255 (49.80%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKA-NINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALL-NAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTE 1445
N +G PL AA + + + +LL +K N+N D+D T LH A L LLL + A +N + + TPT IA G ++++ +L+ S AD+ + D TALHLA L V+ L+ AGAD+ + HLA+ G ++ I ++ V+ +D +A +H+AA H +L+K+ AD+ + TALH+A ++ +L VK L+ A+ + + + T H+A + E
Sbjct: 478 ANAQGATPLHQAAEKRLKGVSEILLSRKTTNVNAKDEDQYTPLHFAAQNGDEALTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVLL-SRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADVDGQTSDGRSPLHLASQRGQYRVARILVELGAN--VHLTSDDLYAPLHVAAETGHTSTSRLLVKHDADIKSRTANGCTALHLASQKGHLPTVKMLLAEGADPESVNHDLRTPCHLAAQNGHCE 729 HSP 3 Score: 93.9745 bits (232), Expect = 1.945e-19 Identity = 66/213 (30.99%), Postives = 116/213 (54.46%), Query Frame = 3
Query: 654 VLRLVDSVGVNTNLKG---MYPLLIAANEGHEDLVNLLLEKK-ANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKAD 1280
V+R++ S G + ++KG L +AA +GH +V LL+++ A+++ DG + LHLA ++++ +L++ GA+V+ ++ P +A G LL+ ++AD+ ++ TALHLA L +V+ LL GAD +N L HLAA +GH ++L+ L R + N+Q + A+H+A H+ + VL++ AD
Sbjct: 564 VVRVLLSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADVDGQTSDGRSPLHLASQRGQYRVARILVELGANVHLTSDDLYAPLHVAAETGHTSTSRLLV-KHDADIKSRTANGCTALHLASQKGHLPTVKMLLAEGADPESVNHDLRTPCHLAAQNGHCEVLKELL-RSCSDVANAQDRNGLTALHLAVSGGHKDAICVLLEGGAD 774 HSP 4 Score: 80.1073 bits (196), Expect = 5.021e-15 Identity = 66/265 (24.91%), Postives = 122/265 (46.04%), Query Frame = 3
Query: 738 EDLVNLL-LEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNS-----SRL----------------------------ESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK 1430
ED+ L+ + + ++++ G LH AV + LL + N N + TP A K + + E+L++ +++ D D+ T LH A + +RL V LL+ GAD+++ K + A HLAA GH I+++ +K+ G V+ Q D + +H+A+ +L++ A+++L + +L LH+A + + S + LV++ A++ + NG TALH+A +
Sbjct: 428 EDIAKLMKILQPQDVDLLLDGGSNLLHYAVSLANEEAVKFLLLSNCNPNLANAQGATPLHQAAEKRLKGVSEILLSRKTTNVNAKDEDQYTPLHFAAQNGDEALTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVLLSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGAD-VDGQTSDGRSPLHLASQRGQYRVARILVELGANVHLTSDDLYAPLHVAAETGHTSTSRLLVKHDADIKSRTANGCTALHLASQ 691 HSP 5 Score: 62.3882 bits (150), Expect = 1.660e-9 Identity = 56/226 (24.78%), Postives = 101/226 (44.69%), Query Frame = 3
Query: 1011 LHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKEL 1688
LH AV+ + E+V+ LL + + L N + H AA + + EI L R VN++ ED++ +H AA N E +L+ A +N + + RT HIA + V+ L+ A++ +K K+ TALH+A LG+ + + + + GA +D + + G++PL + + + R+ ++ H+T +L
Sbjct: 453 LHYAVSLANEEAVKFLLLSNCNPNLANAQGATPLHQAAEKRLKGVSEILLSRKTTN-VNAKDEDQYTPLHFAAQNGDEALTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVLLSRGADVHVKGKDDWTALHLAA--------------WKGHLGI-------------VKLLVKQAGADVDGQTSDGRSPLHLASQRG-QYRVARILVELGANVHLTSDDL 649
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Xenopus
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100170182]) HSP 1 Score: 259.61 bits (662), Expect = 3.146e-74 Identity = 191/634 (30.13%), Postives = 305/634 (48.11%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVKK-------------HKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEVLRLV--DSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM------------------KVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
+L + Q HGGW D M E LT + D+ D V PSG + N +L V+ ++G VQ+ D E KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G++++V L+ V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A+ KV+ T N L M LG G IA +L NGA + I+N KG++PLD+C D +L + L+ ++ + PS SN++E +E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 ELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISNATGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRQDVDVNGQCAGHTAMQAASQNGHVDILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKLL-DFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTWEPSKNTLIMG-----LGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPNL-CKALAKCHKEKSSGQVGSHS-------PS-MISNDSETLEE------------CMVCSDLKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 56.225 bits (134), Expect = 1.563e-7 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 945 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRI 1003
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Xenopus
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100170182]) HSP 1 Score: 256.529 bits (654), Expect = 5.930e-73 Identity = 196/648 (30.25%), Postives = 311/648 (47.99%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVKK-------------HKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVAL----KTGNLSE----------VLRLVD-----SVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM------------------KVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
+L + Q HGGW D M E LT + D+ D V PSG + N +L V+ ++G VQ+ D E KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + D + L+ ER + L L ++G+L+E V ++ D V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A+ KV+ T N L M LG G IA +L NGA + I+N KG++PLD+C D +L + L+ ++ + PS SN++E +E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 ELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISNATGGKLDYNLSFKLV--ERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRQDVDVNGQCAGHTAMQAASQNGHVDILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKLL-DFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTWEPSKNTLIMG-----LGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPNL-CKALAKCHKEKSSGQVGSHS-------PS-MISNDSETLEE------------CMVCSDLKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 875 HSP 2 Score: 56.6102 bits (135), Expect = 1.448e-7 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 985 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRI 1043
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Xenopus
Match: coa7 (cytochrome c oxidase assembly factor 7 (putative) [Source:Xenbase;Acc:XB-GENE-6258829]) HSP 1 Score: 238.81 bits (608), Expect = 4.868e-67 Identity = 191/678 (28.17%), Postives = 331/678 (48.82%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKS-EIVENTELKXXXXXXXXXXXXXXLI-------TD-ERTMRFLVALKTGNLSEVLRLVDSVGVNTNLK--GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLN-AGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM------KVNQTESGFG-NFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSD-------ARSNEAELM---------------------------------------ELRQKLKEMEDEK-----------------ICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
D+ + + Q+ HGGWN M E + TG I + G D V S + + L S +G V+++ + + K LQ HGEW +DM + LG+ +VI++F DGD ++S + + +P + S + E+ L + ++ + S++ TD E ++ GN ++V ++ ++K G L +A++ G+ ++V +LL+ ANI++ D +GDTALH A + N+ + +LL KGA+ NN K T IAV KG +++++L N A +++ DS DT LH A+ + +E L D + N + FN H +A+ G+ + L+R +LV+S+KED F A+H+A +NNH+ + +L+K + D+NL+N +T LH+AV + ++S V LV A+++ +D++G+T +H+ ++ + G G + L A G+ GNI L+ +A +L +GA I+ N++GK+PLD+ D RI LV+ +QK + L P+ + ++ AE + +LRQ E+ D+K C IC D +K++F+CGH C +CS +++ C +C + I +I+I
Sbjct: 285 DVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERG-DVRVQYNSETRWTFHPGALTKQNSFW---VGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCA-INMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILER-ARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNI--ELNVGSAMACFLALSGADINYANHRGKSPLDLIADG----RITHLVKNFAQKFRYQRAILPYIHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQGGLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQI 950
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Xenopus
Match: coa7 (cytochrome c oxidase assembly factor 7 (putative) [Source:Xenbase;Acc:XB-GENE-6258829]) HSP 1 Score: 227.254 bits (578), Expect = 6.426e-63 Identity = 182/619 (29.40%), Postives = 316/619 (51.05%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKS-EIVENTELKXXXXXXXXXXXXXXLI-------TD-ERTMRFLVALKTGNLSEVLRLVDSVGVNTNLK--GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLN-AGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM------KVNQTESGFG-NFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDAR---SNEAELMELRQKLKEMEDEKICKICHDRRLKV-IFECGH-MCCDDCSDNISECHLCSEKILHKIK 1910
D+ + + Q+ HGGWN M E + TG I + G D V S + + L S +G V+++ + + K LQ HGEW +DM + LG+ +VI++F DGD ++S + + +P + S + E+ L + ++ + S++ TD E ++ GN ++V ++ ++K G L +A++ G+ ++V +LL+ ANI++ D +GDTALH A + N+ + +LL KGA+ NN K T IAV KG +++++L N A +++ DS DT LH A+ + +E L D + N + FN H +A+ G+ + L+R +LV+S+KED F A+H+A +NNH+ + +L+K + D+NL+N +T LH+AV + ++S V LV A+++ +D++G+T +H+ ++ + G G + L A G+ GNI L+ +A +L +GA I+ N++GK+PLD+ D RI LV+ +QK + + L+ P R + + L E C +C + + V F C H + C++CS + +C C I K++
Sbjct: 279 DVEILREMQEGHGGWNPKMAEVMARTGTVHRITERG-DVRVQYNSETRWTFHPGALTKQNSFW---VGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCA-INMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILER-ARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNI--ELNVGSAMACFLALSGADINYANHRGKSPLDLIADG----RITHLVKNFAQKF---RYQRLDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAE--CLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLR 880 HSP 2 Score: 94.3597 bits (233), Expect = 2.944e-19 Identity = 101/465 (21.72%), Postives = 192/465 (41.29%), Query Frame = 3
Query: 639 GNLSEVLRLVDSVGVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLK-KGADVNAQNNKKI----------------------------------TPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAE-LDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELME-LRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
GN + V+R++ S G N L L IA N+G ++V +L IN+ D GDT LH A+ + + ++L + D QNN+ T +A L Q+++E+LI D+++ ++ T LHLAV + V L+ GAD+ ++ H+ + H K ++ + +G L+ + + + C L AD+N N ++ L + + + HLV+N A+ + + T +V+ T + N ++A + L L ++ + ++ +C + S R+ ++ +++ E PS S++ ++ME L+ + ++ME+ C IC D +K++F+CGH C +CS +++ C +C + I +I+I
Sbjct: 519 GNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSG--ADINYANHRGKSPLDLIADGR----ITHLVKNFAQKFRYQRLDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVL--VSFFPCQHSI--------------VCEECS--RRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQI 959
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Xenopus
Match: coa7 (cytochrome c oxidase assembly factor 7 (putative) [Source:Xenbase;Acc:XB-GENE-6258829]) HSP 1 Score: 225.713 bits (574), Expect = 1.848e-62 Identity = 182/616 (29.55%), Postives = 313/616 (50.81%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKS-EIVENTELKXXXXXXXXXXXXXXLI-------TD-ERTMRFLVALKTGNLSEVLRLVDSVGVNTNLK--GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLN-AGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM------KVNQTESGFG-NFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKV-IFECGH-MCCDDCSDNISECHLCSEKILHKIK 1910
D+ + + Q+ HGGWN M E + TG I + G D V S + + L S +G V+++ + + K LQ HGEW +DM + LG+ +VI++F DGD ++S + + +P + S + E+ L + ++ + S++ TD E ++ GN ++V ++ ++K G L +A++ G+ ++V +LL+ ANI++ D +GDTALH A + N+ + +LL KGA+ NN K T IAV KG +++++L N A +++ DS DT LH A+ + +E L D + N + FN H +A+ G+ + L+R +LV+S+KED F A+H+A +NNH+ + +L+K + D+NL+N +T LH+AV + ++S V LV A+++ +D++G+T +H+ ++ + G G + L A G+ GNI L+ +A +L +GA I+ N++GK+PLD+ D RI LV+ +QK + EL V S R + C +C + + V F C H + C++CS + +C C I K++
Sbjct: 268 DVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERG-DVRVQYNSETRWTFHPGALTKQNSFW---VGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCA-INMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILER-ARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNI--ELNVGSAMACFLALSGADINYANHRGKSPLDLIADG----RITHLVKNFAQKFR-EQVELDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLR 870 HSP 2 Score: 94.7449 bits (234), Expect = 1.801e-19 Identity = 99/465 (21.29%), Postives = 193/465 (41.51%), Query Frame = 3
Query: 639 GNLSEVLRLVDSVGVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLK-KGADVNAQNNKKI----------------------------------TPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVEN-KAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELME-LRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
GN + V+R++ S G N L L IA N+G ++V +L IN+ D GDT LH A+ + + ++L + D QNN+ T +A L Q+++E+LI D+++ ++ T LHLAV + V L+ GAD+ ++ H+ + H K ++ + +G L+ + + + C L AD+N N ++ L + + VK+ + + +++L + +L +V+ T + N ++A + L L ++ + ++ +C + S R+ ++ +++ E PS S++ ++ME L+ + ++ME+ C IC D +K++F+CGH C +CS +++ C +C + I +I+I
Sbjct: 508 GNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSG--ADINYANHRGKSPLDLIADGRITHLVKNFAQKFREQVELDSPTVSCSLR---RVHTTPNTMTNLSPCSAASPAECLVCSELAVL--VSFFPCQHSI--------------VCEECS--RRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQI 949
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Mouse
Match: Mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:2443157]) HSP 1 Score: 262.692 bits (670), Expect = 1.768e-75 Identity = 190/631 (30.11%), Postives = 309/631 (48.97%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVK-------------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEVLRLV--DSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------------VNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ + ++G VQ+ D E KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G++++V L+ V VN G + A+ GH D++ LLL++ ++ DKDGD A+H A + + ++L + AD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV A+LD++DK+G+T LH A++ V + ++ + K T +G LG G IA +L NGA + I+N KG++PLD+C D SL + L+ ++ + + PS SN++E +E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAW-EPSKNTLIMG-LGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSL-CKALAKCHKEKVSGQVGSRS-------PS-MISNDSETLEE------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 56.225 bits (134), Expect = 1.451e-7 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 945 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRI 1003
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Mouse
Match: Mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:2443157]) HSP 1 Score: 262.692 bits (670), Expect = 1.768e-75 Identity = 190/631 (30.11%), Postives = 309/631 (48.97%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVK-------------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEVLRLV--DSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------------VNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ + ++G VQ+ D E KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G++++V L+ V VN G + A+ GH D++ LLL++ ++ DKDGD A+H A + + ++L + AD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV A+LD++DK+G+T LH A++ V + ++ + K T +G LG G IA +L NGA + I+N KG++PLD+C D SL + L+ ++ + + PS SN++E +E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAW-EPSKNTLIMG-LGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSL-CKALAKCHKEKVSGQVGSRS-------PS-MISNDSETLEE------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 56.225 bits (134), Expect = 1.451e-7 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 945 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRI 1003
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Mouse
Match: Mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:2443157]) HSP 1 Score: 209.149 bits (531), Expect = 2.369e-58 Identity = 153/521 (29.37%), Postives = 262/521 (50.29%), Query Frame = 3
Query: 423 GKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEVLRLV--DSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------------VNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
GK V QI+ D D K+ + +P + + + + + + T E + A G++++V L+ V VN G + A+ GH D++ LLL++ ++ DKDGD A+H A + + ++L + AD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV A+LD++DK+G+T LH A++ V + ++ + K T +G LG G IA +L NGA + I+N KG++PLD+C D SL + L+ ++ + + PS SN++E +E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 2 GKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAW-EPSKNTLIMG-LGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSL-CKALAKCHKEKVSGQVGSRS-------PS-MISNDSETLEE------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 498 HSP 2 Score: 55.0694 bits (131), Expect = 2.870e-7 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 580 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRI 638
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Mouse
Match: Mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:MGI Symbol;Acc:MGI:2679684]) HSP 1 Score: 201.06 bits (510), Expect = 4.353e-54 Identity = 151/556 (27.16%), Postives = 274/556 (49.28%), Query Frame = 3
Query: 327 IGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKS---------EIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVD--SVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLES--VEALLN-AGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMK-YKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQ-------TESGFGNFLKMTSALGVLGNIG-EGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDAR---SNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGH-MCCDDCSDNISECHLCSEKILHKIK 1910
+G V++I D + K LQ HGEW +DM LG+ +V+++F DG+ +++ + + P + + ++ E + D E R +V GN++ L L+ V+T +G L +AA G +LV LLL+ +A++++ D +G+T LH N+ + +LL G V+A+N + T +AV +G +++++L D+++ D+ DT LH A+++ S VE L G D+ N + F H A++ GH + L R +LV+++KED F A+H+AA+NNH VL++ + D+N++N +L++ LH+AV++ +L V LV+ ++ +D+ G+TALHVA++ +Q G L++ S L G G L +A +L GA + N++G++PLD+ + R+L ++ +Q+ +++ P R S + L + C +C + L ++F C H C++C+ + +C C I K++
Sbjct: 294 VGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILC-ERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEG----RVLKALQGCAQR--FRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLR 841 HSP 2 Score: 55.4546 bits (132), Expect = 2.115e-7 Identity = 19/57 (33.33%), Postives = 35/57 (61.40%), Query Frame = 3
Query: 1743 ELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
EL+ + ++ME+ C IC D ++++F+CGH C C ++ C +C + I +I+I
Sbjct: 863 ELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQI 919
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025]) HSP 1 Score: 121.324 bits (303), Expect = 1.215e-27 Identity = 94/353 (26.63%), Postives = 163/353 (46.18%), Query Frame = 3
Query: 684 NTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELM 1742
+ L + L +AA+ GH + LLL+K+AN N +G T LH+A +NR K+ +LL+K GA + A +TP +A G +I+ LL+ N A + + +TALH+A + ++E V LL GA + ++ H+A+ G +I+++ L+ ++ + + +H++A VL++ A +L + T LH+A K L K L++ +A D KNG T LH+A K NQ + IA L+ GA + +G TPL + + + L+++ + H TK L + + + N A+++
Sbjct: 339 DVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHP--DAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQ----------------------------IASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADIL 660 HSP 2 Score: 114.775 bits (286), Expect = 1.449e-25 Identity = 95/359 (26.46%), Postives = 170/359 (47.35%), Query Frame = 3
Query: 699 GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM---KVNQTE--SGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKL 1760
G+ P+ +AA GH ++V LLL+ A+ ++ + G+TALH+A + ++ LL+ GA V+A+ ++ TP IA G +I++LL+ + A ++ T LH++ +++ LL AGA L KK F H+AA G + ++ L+R +S ++ + +H+AA N L+ Y A+ N + T LH+A + + V L++ A + + K+G T+LH+A KVN + + G + LG I + +L++ GA+++ K G TPL + Q H LL+H +P+ +N + + ++L
Sbjct: 410 GLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL-QHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAA--ADSAGKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLH---------------QAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRL 750 HSP 3 Score: 112.849 bits (281), Expect = 4.887e-25 Identity = 91/347 (26.22%), Postives = 152/347 (43.80%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSN 1727
T G PL IAA+ G+ ++ LLL + A ++ ++G T LH+A + LLL +G ++A+ +TP A G ++ELL+ A L + + LH+A +E V+ LL A + + A H+AA GH ++ ++ L + N++ + F +H+A N + +L+KY A + T +H+A +L+ V L++N A D+ + G TALH+A + Q E + L+ NGA +D + + +TPL I + KT I+ L LL+H P A +N
Sbjct: 208 TTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLL-ERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANP--NARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVE----------------------------VVRCLLRNGALVDARAREEQTPLHIASRLG-KTEIVQL--------------LLQHMAHPDAATTN 508 HSP 4 Score: 112.079 bits (279), Expect = 9.047e-25 Identity = 85/279 (30.47%), Postives = 141/279 (50.54%), Query Frame = 3
Query: 618 FLVALKTGNLSEVLR-LVDSVGVNT-NLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRD------GEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK 1430
FL A + GNL +V+ L + +NT N G+ L +AA EGH LV LL + ++++ A K G+TALH+A + ++ +L+K+GA++NAQ+ TP +A + D+++ L+ N A+ S D T L +A+ ++V LL K A H+AA K + L+ D + +VN E F +H+AA + + +L+ A ++ T LH+A KR + VK L++ ++D K ++G T LH A +
Sbjct: 14 FLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLL-ENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTK----GKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAAR 287 HSP 5 Score: 104.76 bits (260), Expect = 1.625e-22 Identity = 68/222 (30.63%), Postives = 113/222 (50.90%), Query Frame = 3
Query: 696 KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHL 1361
KG PL +AA G D+ LLL+++A + A K+G T LH+A +N+ ++ LL GA+ N + +TP +A +G D++ LL+ A++ + T+LHLA ++ + L GAD K + +A G+ K++ LK+ VN++ ++ + +H AA H H + VL+++ A N TAL IA + Y+S V L
Sbjct: 541 KGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLL-DKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGAN--VNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 759 HSP 6 Score: 80.1073 bits (196), Expect = 7.758e-15 Identity = 48/135 (35.56%), Postives = 74/135 (54.81%), Query Frame = 3
Query: 678 GVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGA 1073
G TN +G+ PL +A+ EGH D+V LLL+K ANI+++ K G T+LHLA E++ + D+L K GAD +A TP ++A G+ ++ L+ A+++ + T LH A + LL GA
Sbjct: 598 GAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLL-KQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGA 731 HSP 7 Score: 70.4774 bits (171), Expect = 7.294e-12 Identity = 74/294 (25.17%), Postives = 123/294 (41.84%), Query Frame = 3
Query: 993 SDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKA-----------------------------ELDLKDKNGNTALHVAMKVNQTESGF-----GNFLKMTSALGVLGNIGEGLDKLE--------DIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMEL 1748
SD + + A + L+ V L G DI N+ NA HLAA GH +++ L R V+S + A+H+A++ + VL+K A++N Q+ T L++A + ++ VK+L+EN A E D K K ALH+A + + T+S + + S + V G L ++A L+ GA++D G TPL + + + L++R Q T+ L P+ + ARS +++EL
Sbjct: 8 SDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSS--VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLT--PLHCA-ARSGHDQVVEL 296 HSP 8 Score: 52.7582 bits (125), Expect = 1.850e-6 Identity = 45/162 (27.78%), Postives = 68/162 (41.98%), Query Frame = 3
Query: 1191 QKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTE-----SGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQ 1661
QK D A+ AA + + +K D+N N ALH+A K ++ V+ L+ + +D K GNTALH+A Q E G + S G + D+ YL+ENGA+ G TPL + + L+E D++
Sbjct: 6 QKSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTK 167
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. UniProt/SwissProt
Match: sp|Q80SY4|MIB1_MOUSE (E3 ubiquitin-protein ligase MIB1 OS=Mus musculus OX=10090 GN=Mib1 PE=1 SV=1) HSP 1 Score: 262.692 bits (670), Expect = 1.236e-74 Identity = 190/631 (30.11%), Postives = 309/631 (48.97%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVK-------------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEVLRLV--DSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------------VNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ + ++G VQ+ D E KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G++++V L+ V VN G + A+ GH D++ LLL++ ++ DKDGD A+H A + + ++L + AD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV A+LD++DK+G+T LH A++ V + ++ + K T +G LG G IA +L NGA + I+N KG++PLD+C D SL + L+ ++ + + PS SN++E +E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAW-EPSKNTLIMG-LGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSL-CKALAKCHKEKVSGQVGSRS-------PS-MISNDSETLEE------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 56.225 bits (134), Expect = 1.015e-6 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 945 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRI 1003
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. UniProt/SwissProt
Match: sp|Q86YT6|MIB1_HUMAN (E3 ubiquitin-protein ligase MIB1 OS=Homo sapiens OX=9606 GN=MIB1 PE=1 SV=1) HSP 1 Score: 260.766 bits (665), Expect = 6.175e-74 Identity = 189/631 (29.95%), Postives = 309/631 (48.97%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVK-------------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEVLRLV--DSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------------VNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ + ++G VQ+ D E KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G++++V L+ V VN G + A+ GH D++ LLL++ ++ DKDGD A+H A + + ++L + AD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV A+LD++DK+G+T LH A++ V + ++ + K T +G LG G IA +L NGA + I+N KG++PLD+C D +L + L+ ++ + + PS SN++E +E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAW-EPSKNTLIMG-LGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPNL-CKALAKCHKEKVSGQVGSRS-------PS-MISNDSETLEE------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 56.225 bits (134), Expect = 1.051e-6 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 945 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRI 1003
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. UniProt/SwissProt
Match: sp|Q804S5|MIB1_DANRE (E3 ubiquitin-protein ligase mib1 OS=Danio rerio OX=7955 GN=mib1 PE=1 SV=1) HSP 1 Score: 260.381 bits (664), Expect = 1.288e-73 Identity = 192/630 (30.48%), Postives = 305/630 (48.41%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKI-------------GSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEV---LRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGV-------------LGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ ++ G VQ+ D + KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G+L++V L+ D V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A++ + T S M V LG G IA +L NGA + I+N KG++PLD+C D SL + L+ ++ + + PS +NE L+E C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPD-VDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALR-HHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSL-CKALAKCHKEKTSGQVGSRS-------PSLNSNNET--------LEE------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 861 HSP 2 Score: 56.6102 bits (135), Expect = 6.552e-7 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 969 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRI 1027
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. UniProt/SwissProt
Match: sp|Q6GNY1|MIB1_XENLA (E3 ubiquitin-protein ligase mib1 OS=Xenopus laevis OX=8355 GN=mib1 PE=2 SV=1) HSP 1 Score: 258.84 bits (660), Expect = 3.204e-73 Identity = 188/631 (29.79%), Postives = 308/631 (48.81%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVKK-------------HKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEVLRLV--DSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------------VNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
+L + Q HGGW D M E LT + D+ D V PSG + N +L V+ ++G VQ+ D E KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G++++V L+ V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ + S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A+L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A++ V + ++ K T +G LG G IA +L NGA + ++N KG++PLD+C D +L + L+ ++ + PS +N++E +E C +C D + +F CGH+ C CS + +C LC E++ + KI
Sbjct: 257 ELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDIERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISNATGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVDDLLKRQDVDVNGQCAGHTAMQAASQNGHVDILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKKLL-DFSCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTTWEPSKNTLIMG-LGTQGAEKKSAASIACFLAANGADLAVRNKKGQSPLDLCPDPNL-CKTLAKCHKEKSSGQVGSHS-------PS-MINNDSETLEE------------CMVCSDLKRDTLFGPCGHIATCSLCSPRVKKCLLCKEQVQSRTKI 864 HSP 2 Score: 56.225 bits (134), Expect = 8.496e-7 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 946 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRI 1004
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. UniProt/SwissProt
Match: sp|Q9VUX2|MIB_DROME (E3 ubiquitin-protein ligase mind-bomb OS=Drosophila melanogaster OX=7227 GN=mib1 PE=1 SV=3) HSP 1 Score: 254.603 bits (649), Expect = 4.382e-71 Identity = 203/691 (29.38%), Postives = 327/691 (47.32%), Query Frame = 3
Query: 81 GANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLV----PFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHP----KTIKSEIVENTELKXXXXXXXXXXXXXXL----ITDERTMRFLVALKTGNLSEVLRLVDS----------------VGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFL-KRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVN--------QTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSL-KTRILSLVER----------------------------DSQKHHITKKELLEHPVQPSDARSNEAEL----MELRQKLKEMEDEKI-----CKICHDRRLKVIFE-CGHM-CCDDCSDNISECHLCSEKILHKIKIN 1916
G F I KV + + DL + Q HGGW D M ECL+ ++ D+ D V SG + N +L P + + ++G V++ D E K+LQ HGEW + M+ LGK V Q++ D D K+ N + +P K S + + S + + + L ++ + T F+ A G + + V VN G L A+ GH +++ +LL ++ I DKDGD A+H A + + ++L K GAD+NA+N ++ T IAV KG ++++ L+T S+ DS+ DT LH A++ E + LL+ GADI L N FNA H AA+ G+ ++I L K + +V +K+D + A+H+AA+NNH +L+ KA+++ QN+ L+TALH+AV+R+++ VK LV++ A+L++ DK+G+T LH A++ + Q GFG L +G+ IA +L NGA + +KN K +TPLD+C D +L KT + ER S ++ L P+ + S+ L E+ Q L E + C +C D + +F+ CGH+ CC+ C+ + +C +C E + + KI+
Sbjct: 334 GKGPHGFQIGDKVTV-DLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSSERLSAILKKLFEPNVSGDATEEFVKAAANGFAARCEEYLAGAAQPSTSSASPSSGPDVNVNGVFAGHTALQAASQNGHIEVIQVLLRHAVDVEIEDKDGDRAVHHAAFGDEAAVIEILAKAGADLNARNKRRQTSLHIAVNKGHLNVVKTLLTL-GCHPSLQDSEGDTPLHDAISKEHDEMLSLLLDFGADITLNNNNGFNALHHAALKGNPSAMKILLTKTNRPWIVEEKKDDGYTALHLAALNNHVEIAELLVHMGKANMDRQNVNLQTALHLAVERQHVQIVKLLVQDGADLNIPDKDGDTPLHEALRHHTLSQLKQLQDVEGFGKLL-----MGLRN--ANNKKASASIACFLAANGADLTLKNRKQQTPLDLCPDPNLCKTLVKCYNERKTDDSELPGNVAGTSSSARARAASGSLNQSSSVNMPLSSLAASSTFPAASSSSIFALNGIANEMSQSLHEDPPKSSASLDECLVCSDAKRDTVFKPCGHVSCCETCAPRVKKCLICRETVSSREKID 1015
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. TrEMBL
Match: A0A0P5YTS7 (E3 ubiquitin-protein ligase mib1 OS=Daphnia magna OX=35525 PE=4 SV=1) HSP 1 Score: 273.863 bits (699), Expect = 1.599e-76 Identity = 207/651 (31.80%), Postives = 322/651 (49.46%), Query Frame = 3
Query: 84 ANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELL-----VPFKSVK------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXL----ITDERTMRFLVALKTGN---LSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------VNQTESGFGNFLKMTSA-----LGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNE---AELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHM-CCDDCSDNISECHLCSEKILHKIKI 1913
+ + F I +V + + DL + Q HGGW D M ECL TG I++D D V PSG + N +L P + +G VQ+ D E K+LQ HGEW E M LGK V QI+ D D K+ + +P + ++I + L + ++ + + L +T + + A G+ + E+L+ VD+ VN G L A+ GH +++ +LL KA++ I DKDGD A+H A + + ++L + GAD+NA+N ++ T IAV KG +++ L+ S+ DS+ DTALH A++ R + + L++ ADI L N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L++ +A L+LQN+ L+TALH+AV+R++ V+ LV L+L DK+G+T LH A++ + + G G TS L LG G IA +L NGA + IKN KG TPLD+C D +L + S T+ E H D S E L ++ L + DE C +C D + +F CGH+ CC C+ + +C +C + I + KI
Sbjct: 247 SGLHGFAIGDQVNV-DLDLELVQTLQHGHGGWTDGMFECLGTTGTVVGIDED-HDIVVSYPSGNRWTFNPTILRVVVTSPPPGTSNVDVQLQFAVGDLVQICSDLERIKILQRGHGEWAEAMAPTLGKVGRVQQIYHDNDLKVEVCGTSWTYNPAAV-TKIASDGSLPGASNGENLSALLKKLFETHVTGDVHEELVKAAANGDTQKVEEILKRVDA-DVNGVFAGHTALQAASQNGHLEVIKVLLRYKADVEIEDKDGDRAVHHAAFGDEPSVMEVLAQSGADLNARNKRRQTALHIAVNKGHVGVVKTLLEL-GCHPSLQDSEGDTALHDAISKKRDDMLTLLIDHNADITLTNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVRAGRAQLDLQNVNLQTALHLAVERQHTQIVRLLVRENCSLNLADKDGDTPLHEALRHHTLSQLRQLQDLQDGSGKTSTATSGHNYSLLMGLGTQGANKKSSASIACFLAANGADLTIKNKKGLTPLDLCPDPNLCRALTKCYGEKS-----TRGECGAH----GDTLSQEMGAVALTDMACDLTDTADE--CMVCSDMKRDTLFGPCGHVNCCSACAPRVKKCLVCKDPITTRTKI 881
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. TrEMBL
Match: A0A0P5H436 (E3 ubiquitin-protein ligase mib1 OS=Daphnia magna OX=35525 PE=4 SV=1) HSP 1 Score: 271.552 bits (693), Expect = 4.395e-76 Identity = 205/651 (31.49%), Postives = 321/651 (49.31%), Query Frame = 3
Query: 84 ANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELL-----VPFKSVK------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXL----ITDERTMRFLVALKTGN---LSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------VNQTESGFGNFLKMTSA-----LGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNE---AELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHM-CCDDCSDNISECHLCSEKILHKIKI 1913
+ + F I +V + + DL + Q HGGW D M ECL TG I++D D V PSG + N +L P + +G VQ+ D E K+LQ HGEW E M LGK V QI+ D D K+ + +P + ++I + L + ++ + + L +T + + A G+ + E+L+ VD+ VN G L A+ GH +++ +LL KA++ I DKDGD A+H A + + ++L + GAD+NA+N ++ T IAV KG +++ L+ S+ DS+ DT LH A++ R + + L++ ADI L N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L++ +A L+LQN+ L+TALH+AV+R++ V+ LV L+L DK+G+T LH A++ + + G G TS L LG G IA +L NGA + IKN KG TPLD+C D +L + S T+ E H D S E L ++ L + DE C +C D + +F CGH+ CC C+ + +C +C + I + K+
Sbjct: 247 SGLHGFAIGDQVNV-DLDLELVQTLQHGHGGWTDGMFECLGTTGTVVGIDED-HDIVVSYPSGNRWTFNPTILRVVVTSPPPGTSNVDVQLQFAVGDLVQICSDLERIKILQRGHGEWAEAMAPTLGKVGRVQQIYHDNDLKVEVCGTSWTYNPAAV-TKIASDGSLPGASNGENLSALLKKLFETHVTGDVHEELVKAAANGDTQKVEEILKRVDA-DVNGVFAGHTALQAASQNGHLEVIKVLLRYKADVEIEDKDGDRAVHHAAFGDEPSVMEVLAQSGADLNARNKRRQTALHIAVNKGHVGVVKTLLEL-GCHPSLQDSEGDTPLHDAISKKRDDMLTLLIDHNADITLTNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVRAGRAQLDLQNVNLQTALHLAVERQHTQIVRLLVRENCSLNLADKDGDTPLHEALRHHTLSQLRQLQDLQDGSGKTSTATSGHNYSLLMGLGTQGANKKSSASIACFLAANGADLTIKNKKGLTPLDLCPDPNLCRALTKCYGEKS-----TRGECGAH----GDTLSQEMGAVALTDMACDLTDTADE--CMVCSDMKRDTLFGPCGHVNCCSACAPRVKKCLVCKDPITTRTKV 881
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. TrEMBL
Match: A0A0P5QQ02 (E3 ubiquitin-protein ligase mib1 OS=Daphnia magna OX=35525 PE=4 SV=1) HSP 1 Score: 271.937 bits (694), Expect = 2.877e-75 Identity = 206/651 (31.64%), Postives = 321/651 (49.31%), Query Frame = 3
Query: 84 ANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELL-----VPFKSVK------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXL----ITDERTMRFLVALKTGN---LSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK---------VNQTESGFGNFLKMTSA-----LGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNE---AELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHM-CCDDCSDNISECHLCSEKILHKIKI 1913
+ + F I +V + + DL + Q HGGW D M ECL TG I++D D V PSG + N +L P + +G VQ+ D E K+LQ HGEW E M LGK V QI+ D D K+ + +P + ++I + L + ++ + + L +T + + A G+ + E+L+ VD+ VN G L A+ GH +++ +LL KA++ I DKDGD A+H A + + ++L + GAD+NA+N ++ T IAV KG +++ L+ S+ DS+ DT LH A++ R + + L++ ADI L N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L++ +A L+LQN+ L+TALH+AV+R++ V+ LV L+L DK+G+T LH A++ + + G G TS L LG G IA +L NGA + IKN KG TPLD+C D +L + S T+ E H D S E L ++ L + DE C +C D + +F CGH+ CC C+ + +C +C + I + KI
Sbjct: 247 SGLHGFAIGDQVNV-DLDLELVQTLQHGHGGWTDGMFECLGTTGTVVGIDED-HDIVVSYPSGNRWTFNPTILRVVVTSPPPGTSNVDVQLQFAVGDLVQICSDLERIKILQRGHGEWAEAMAPTLGKVGRVQQIYHDNDLKVEVCGTSWTYNPAAV-TKIASDGSLPGASNGENLSALLKKLFETHVTGDVHEELVKAAANGDTQKVEEILKRVDA-DVNGVFAGHTALQAASQNGHLEVIKVLLRYKADVEIEDKDGDRAVHHAAFGDEPSVMEVLAQSGADLNARNKRRQTALHIAVNKGHVGVVKTLLEL-GCHPSLQDSEGDTPLHDAISKKRDDMLTLLIDHNADITLTNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVRAGRAQLDLQNVNLQTALHLAVERQHTQIVRLLVRENCSLNLADKDGDTPLHEALRHHTLSQLRQLQDLQDGSGKTSTATSGHNYSLLMGLGTQGANKKSSASIACFLAANGADLTIKNKKGLTPLDLCPDPNLCRALTKCYGEKS-----TRGECGAH----GDTLSQEMGAVALTDMACDLTDTADE--CMVCSDMKRDTLFGPCGHVNCCSACAPRVKKCLVCKDPITTRTKI 881
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. TrEMBL
Match: A0A4Y2LV21 (E3 ubiquitin-protein ligase MIB1 OS=Araneus ventricosus OX=182803 GN=MIB1 PE=4 SV=1) HSP 1 Score: 268.47 bits (685), Expect = 6.596e-75 Identity = 205/658 (31.16%), Postives = 327/658 (49.70%), Query Frame = 3
Query: 84 ANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSV--------------KKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXL----ITDERTMRFLVALKTGNLS---EVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGS----QDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK------VNQTES-------GFGNFLKMTSALGVLGNIG-EGLDKLED--IAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKIN 1916
A I F I V + + +L + Q HGGW D M ECL TG I++D SD V PSG + N +L + ++ +G VQ+ +D E K+LQ HGEW E M LGK V QI+ D D K+ + +P + +++ + + S + + + L ++ + + A G+ E+L+ D+ VN L A+ GH +++ +LL AN+ I DKDGD A+H A + + +LL + GAD+NA+N ++ TP IAV KG + ILEL S+ DS+ DT LH A++ R + + LL+ GADI L N FNA H AA+ G+ + I L + +VN +K+D + A+H+AA+NNH +L++ KA++N QN+ L+T LH+AV+R++ V+ LV K +L++ DK+G+T LH A++ + Q + G N ++ +L +G + DK + IA +L+ NGA++ IKN KG+TPLD+C D +L + + + + IT P + + NE L+E C +C D + +F CGH+ C CS + +C +C E + + K++
Sbjct: 247 AGIHGFQIGDNVNV-DLELEIVQSLQHRHGGWTDGMFECLGTTGTVVGIDED-SDIVVSYPSGNRWTFNPAVLTKVNTAVSTSSLISSDGGTAQQFTVGDLVQICRDLERIKILQRGHGEWAEAMLPTLGKIGRVQQIYHDNDLKVEVCGTSWTYNPAAV-TKVASDGAIPGTSSGERLSALLKKLFETHVSGDVNEELVKAAANGDAQKCEEILKRSDA-DVNGVFASHTALQAASQNGHIEVIKILLRHNANVEIEDKDGDRAVHHAAFGDEPGVMELLARAGADLNARNKRRQTPLHIAVNKGHIGVVRSILEL-----GCRPSLQDSEGDTPLHDAISKKRDDMLTLLLDHGADICLTNNNGFNALHHAALRGNPSAMRILLSKLPRNWVVNEKKDDGYTALHLAALNNHVEVAELLVQQGKANMNQQNVNLQTPLHLAVERQHTQIVRLLVREKCDLNIPDKDGDTPLHEALRHHTLSQLRQLQDMQEGGKVGISNESTISGTSELLMGLGTQSSDKKSNASIACFLVSNGANLHIKNKKGQTPLDLCPDPNLCKALTKCYKEHANEDSIT-------PSNTTPVQDNET--------LEE------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLMCKEPVQSRTKVS 874
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. TrEMBL
Match: A0A0C9RRG6 (MIB1 protein OS=Fopius arisanus OX=64838 GN=MIB1 PE=4 SV=1) HSP 1 Score: 269.626 bits (688), Expect = 1.325e-74 Identity = 200/619 (32.31%), Postives = 314/619 (50.73%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECL--TGPCKLINDDGSDATVMGPSGMIYRLNSELL------VPFKSV----KKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLI-------TDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFL-KRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIG-EGLDKLED--IAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFE-CGHM-CCDDCSDNISECHLCSEKILHKIKI 1913
+L + Q HG W D M ECL TG I++D D V PSG + N +L VP + + +G VQ+ D E+ KLLQ HGEW E M LGK V QI+ DGD KI + + +P+ I + + + + + + + L +E ++ + E L+ +D+ VN G L A++ GH D++ +LL KA++ I DKDGD A+H A + + L+ GAD+NA+N ++ T IAV KG ++ L+ S+ D++ DT LH A++ R + + LL+ ADI N FNA H AA+ G+ + I L K +V+ +K+D + A+H+AA+NNH +L + KADL+LQN+ L+TALH+AV+R++ V+ LV A +++ DK+G+T LH A++ + T S + +L +G +G DK IA +L +GA +++KN KG+TPLD+C D +L + L+ ++ + H I P PS A DE C +C D + ++F CGH+ CC+ C+ + +C +C E + +IKI
Sbjct: 263 ELEIVQSLQHGHGSWTDGMFECLGTTGIVVSIDED-HDIVVSYPSGNRWTFNPAVLSKVQTPVPVATSTSDNQTFAVGDLVQICNDLEKIKLLQRGHGEWAEAMAPTLGKIGRVQQIYHDGDLKIEVCSTSWTYNPQAIIKVASSDGSVPGNSNGERLSALLKKLFETHVSGDVNEELVKSAANGDSAKCEECLKRLDA-DVNGVFAGHTALQAASHNGHLDVIKILLRYKADVEIEDKDGDRAVHHAAFGDESGVMALVAGAGADLNARNKRRQTALHIAVNKGHAGVVRTLLEL-GCHPSLQDAEGDTPLHDAISKKRDDMLALLLDYAADITRTNNTGFNALHHAALRGNPSAMRILLTKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLARVGKADLDLQNVNLQTALHLAVERQHTQIVRLLVREGANVNVADKDGDTPLHEALR-HHTLSQLRQLQDVQDVGRLLMGLGAQGQDKKSSSFIACFLAAHGADLELKNKKGQTPLDLCPDPNL-CKTLTNCHKNKESHDI-------EPATPSAAI-----------------DE--CLVCSDGKRDMLFSPCGHVSCCNACAPRVKKCLICRENVASRIKI 850
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Cavefish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 258.07 bits (658), Expect = 7.187e-74 Identity = 190/630 (30.16%), Postives = 303/630 (48.10%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKI-------------GSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEV---LRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGV-------------LGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L + ++ G VQ+ D + KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G+L++V L+ D V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A++ + T S M V LG G IA +L NGA + I+N KG++PLD+C D SL + L+ ++ + + PS +NE L+E C +C D + +F CGH+ C CS + +C +C +++ + KI
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPD-VDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALR-HHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSL-CKALAKCHKEKTSGQVGSRS-------PSLNSNNET--------LEE------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKDQVQSRTKI 861 HSP 2 Score: 56.6102 bits (135), Expect = 8.896e-8 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 945 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRI 1003
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Cavefish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 257.684 bits (657), Expect = 1.086e-73 Identity = 190/630 (30.16%), Postives = 303/630 (48.10%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKI-------------GSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEV---LRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGV-------------LGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L + ++ G VQ+ D + KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G+L++V L+ D V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A++ + T S M V LG G IA +L NGA + I+N KG++PLD+C D SL + L+ ++ + + PS +NE L+E C +C D + +F CGH+ C CS + +C +C +++ + KI
Sbjct: 254 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPD-VDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALR-HHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSL-CKALAKCHKEKTSGQVGSRS-------PSLNSNNET--------LEE------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKDQVQSRTKI 858 HSP 2 Score: 56.6102 bits (135), Expect = 7.476e-8 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 966 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRI 1024
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310]) HSP 1 Score: 238.81 bits (608), Expect = 5.406e-67 Identity = 179/611 (29.30%), Postives = 299/611 (48.94%), Query Frame = 3
Query: 159 QKNHGGWNDLMKE--CLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSE---------IVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLK--GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEAL-LNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSAL-------GVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDA---RSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFE-CGH-MCCDDCSDNISECHLCSEKILHKIK 1910
Q+ HGGWN M E C G I D G D V + + + + L V +G V+++ D E K LQ HGEW + M + LG+ +V++++ DGD +++ + + +P + + EN + + R ++ G+ ++V LV ++K G L +AA++GH ++V +LL+ ++I D+DGD ALH N+ ++ LLL KGA+VN NN T IAV KG D++ +L T ++AD+++ DS DT LH A+ +E L + D N + FN H AA+ G++ E+ L R +L + +KED F+A+H+AA+NNH +L+K + D N++N +T L +AV + +++ V LV A+++ +D+ G+TA+H A+ Q + + SAL G+LGN L+ IA +L + GA I+ N+KGK+PLD+ D++ L L+ S+KH + + + + S + R + + C IC + L V+F C H + C++C+ + +C C I KI+
Sbjct: 332 QEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALT---KVNTFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKLLSQSTEQDNPGRLVIEAAHGSATKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGNT--ELNVGAAIACFLAQEGADINYANHKGKSPLDLVTDST----TLQLIRSFSEKHRLQQLQAITCGTSSSSSSLRRVHTTPNTMTNLAMPSAPGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIR 930 HSP 2 Score: 95.1301 bits (235), Expect = 1.013e-19 Identity = 115/478 (24.06%), Postives = 202/478 (42.26%), Query Frame = 3
Query: 639 GNLSEVLRLVDSVGVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLL-LKKGADVNAQNNK-------------KITPTMI---------------------AVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLA--------AISGHEK-ILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM--KVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKK---ELLEHPVQPSDARSNEAELME-LRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
GN +E+ RL+ S G N NL L IA N+G D+V +L E A++N+ D GDT LH A+ ++ + ++L + D QNN+ K+ MI A L +D+ E+L+ D +I ++ T L LAV + V L++ GAD+ +++ A H A +S E+ ++ + L+ + + + AAI C L + AD+N N + ++ L + L ++ E L+ T+ + +V+ T + N L M SA G E L ++A+ ++ + + + + C +K ++ ITKK + E P+ S + L+E L+ + ++ME+ C IC D ++K++F+CGH C DCS + C +C + I +I++
Sbjct: 564 GNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVLTEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSRARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGNTELNVGAAIA---------CF--LAQEGADINYANHKGKSPLDLVTDSTTLQLIRSFSEKHRLQQLQAITCGTSSSSSSLRRVHTTPNTMTN-LAMPSAPGP----SECL-ICSELALLVLFSPCQHSVACEECAHRMKKC----IKCQVT-----------ITKKIRQDQTEVECSPNSDNSEQHNLLEQLQSRYRQMEERITCPICIDNQIKLVFQCGHASCIDCSAALKTCPICRQTIRERIQL 1009
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310]) HSP 1 Score: 238.039 bits (606), Expect = 5.880e-67 Identity = 180/610 (29.51%), Postives = 297/610 (48.69%), Query Frame = 3
Query: 159 QKNHGGWNDLMKE--CLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSE---------IVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLK--GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEAL-LNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSAL-------GVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHI--TKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFE-CGH-MCCDDCSDNISECHLCSEKILHKIK 1910
Q+ HGGWN M E C G I D G D V + + + + L V +G V+++ D E K LQ HGEW + M + LG+ +V++++ DGD +++ + + +P + + EN + + R ++ G+ ++V LV ++K G L +AA++GH ++V +LL+ ++I D+DGD ALH N+ ++ LLL KGA+VN NN T IAV KG D++ +L T ++AD+++ DS DT LH A+ +E L + D N + FN H AA+ G++ E+ L R +L + +KED F+A+H+AA+NNH +L+K + D N++N +T L +AV + +++ V LV A+++ +D+ G+TA+H A+ Q + + SAL G+LGN L+ IA +L + GA I+ N+KGK+PLD+ D++ L L+ S+KH + L + +N A + C IC + L V+F C H + C++C+ + +C C I KI+
Sbjct: 277 QEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALT---KVNTFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKLLSQSTEQDNPGRLVIEAAHGSATKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGNT--ELNVGAAIACFLAQEGADINYANHKGKSPLDLVTDST----TLQLIRSFSEKHRYVVSSSSSLRRVHTTPNTMTNLA--------MPSAPGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIR 866 HSP 2 Score: 94.7449 bits (234), Expect = 1.227e-19 Identity = 114/476 (23.95%), Postives = 201/476 (42.23%), Query Frame = 3
Query: 639 GNLSEVLRLVDSVGVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLL-LKKGADVNAQNNK-------------KITPTMI---------------------AVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLA--------AISGHEK-ILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKK---ELLEHPVQPSDARSNEAELME-LRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
GN +E+ RL+ S G N NL L IA N+G D+V +L E A++N+ D GDT LH A+ ++ + ++L + D QNN+ K+ MI A L +D+ E+L+ D +I ++ T L LAV + V L++ GAD+ +++ A H A +S E+ ++ + L+ + + + AAI C L + AD+N N + ++ L + L ++ E K + ++ +V+ T + N L M SA G E L ++A+ ++ + + + + C +K ++ ITKK + E P+ S + L+E L+ + ++ME+ C IC D ++K++F+CGH C DCS + C +C + I +I++
Sbjct: 509 GNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVLTEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSRARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGNTELNVGAAIA---------CF--LAQEGADINYANHKGKSPLDLVTDSTTLQLIRSFSE-------KHRYVVSSSSSLRRVHTTPNTMTN-LAMPSAPGP----SECL-ICSELALLVLFSPCQHSVACEECAHRMKKC----IKCQVT-----------ITKKIRQDQTEVECSPNSDNSEQHNLLEQLQSRYRQMEERITCPICIDNQIKLVFQCGHASCIDCSAALKTCPICRQTIRERIQL 945
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310]) HSP 1 Score: 238.424 bits (607), Expect = 6.298e-67 Identity = 179/611 (29.30%), Postives = 299/611 (48.94%), Query Frame = 3
Query: 159 QKNHGGWNDLMKE--CLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSE---------IVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLK--GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEAL-LNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSAL-------GVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDA---RSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFE-CGH-MCCDDCSDNISECHLCSEKILHKIK 1910
Q+ HGGWN M E C G I D G D V + + + + L V +G V+++ D E K LQ HGEW + M + LG+ +V++++ DGD +++ + + +P + + EN + + R ++ G+ ++V LV ++K G L +AA++GH ++V +LL+ ++I D+DGD ALH N+ ++ LLL KGA+VN NN T IAV KG D++ +L T ++AD+++ DS DT LH A+ +E L + D N + FN H AA+ G++ E+ L R +L + +KED F+A+H+AA+NNH +L+K + D N++N +T L +AV + +++ V LV A+++ +D+ G+TA+H A+ Q + + SAL G+LGN L+ IA +L + GA I+ N+KGK+PLD+ D++ L L+ S+KH + + + + S + R + + C IC + L V+F C H + C++C+ + +C C I KI+
Sbjct: 338 QEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALT---KVNTFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKLLSQSTEQDNPGRLVIEAAHGSATKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGNT--ELNVGAAIACFLAQEGADINYANHKGKSPLDLVTDST----TLQLIRSFSEKHRLQQLQAITCGTSSSSSSLRRVHTTPNTMTNLAMPSAPGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIR 936 HSP 2 Score: 94.7449 bits (234), Expect = 1.118e-19 Identity = 115/478 (24.06%), Postives = 202/478 (42.26%), Query Frame = 3
Query: 639 GNLSEVLRLVDSVGVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLL-LKKGADVNAQNNK-------------KITPTMI---------------------AVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLA--------AISGHEK-ILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM--KVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKK---ELLEHPVQPSDARSNEAELME-LRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
GN +E+ RL+ S G N NL L IA N+G D+V +L E A++N+ D GDT LH A+ ++ + ++L + D QNN+ K+ MI A L +D+ E+L+ D +I ++ T L LAV + V L++ GAD+ +++ A H A +S E+ ++ + L+ + + + AAI C L + AD+N N + ++ L + L ++ E L+ T+ + +V+ T + N L M SA G E L ++A+ ++ + + + + C +K ++ ITKK + E P+ S + L+E L+ + ++ME+ C IC D ++K++F+CGH C DCS + C +C + I +I++
Sbjct: 570 GNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVLTEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSRARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGNTELNVGAAIA---------CF--LAQEGADINYANHKGKSPLDLVTDSTTLQLIRSFSEKHRLQQLQAITCGTSSSSSSLRRVHTTPNTMTN-LAMPSAPGP----SECL-ICSELALLVLFSPCQHSVACEECAHRMKKC----IKCQVT-----------ITKKIRQDQTEVECSPNSDNSEQHNLLEQLQSRYRQMEERITCPICIDNQIKLVFQCGHASCIDCSAALKTCPICRQTIRERIQL 1015
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008505.1 (pep scaffold:Pmarinus_7.0:GL477211:28706:49738:-1 gene:ENSPMAG00000007672.1 transcript:ENSPMAT00000008505.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 156.377 bits (394), Expect = 3.324e-40 Identity = 138/521 (26.49%), Postives = 242/521 (46.45%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVK-------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLI----TDERTMRFLVALKTGNLSEVLRLVD--SVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGA-DIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK----------VNQTESGFGNFLKMTSALGV-----LGNIGEG-LDKLEDIAIYLIENGASIDIKNNKGKTPLD 1601
D T++ Q++HGG++ M P K+ D D V+ +G Y N + L+ K + + + LI L+ + W + ++ LG+ V+++ GD +++ I+ +P + + ++ +L D + D +I TD + + GN+ V +LV +N +G L +AA++GH D+ LL+ A+ I D G+TALH A H N + LL+ A VN++N+ T +A KG ++ L + D++ DS +++ +H A+ + E +EAL + D+ N+ F LAA G + ++ L + KLV+ QK D FA +H+AA N + +L+K ++ +N + +TAL +A R ++ V LV +LD++D+ GNTALH+ +K + Q +S T L + G G G + E +A +L++ GA++ N +GK P D
Sbjct: 250 DGQTLMVLQQDHGGFSVAMALIAARPGKVAGLIDNGDVLVVYSNGGKYIYNPKCLIKVKMAQCISHLQLRQSFWIVIALIFSPSCIALINDDSINWMKMVKPTLGRVGRVVKVLPSGDLRVALGGHIWNFNPACVSTADPKSLDLAETSEDSAADILKERVILVWDTDLNKKQLISEAAQGNVGNVQKLVQLFPGQINAQQEGRTALHVAAHQGHIDVARALLKAGASTEIGDDKGNTALHYAAHGNEAAMAGLLVSHRARVNSRNHAGRTALHLAARKGYGEVARTL-CEHACDVNTQDSTKNSPIHDAIAENYEEVIEALTSVKTLDLERQNESGFTPLQLAAFKGSKLAVQRILAKS-PKLVDEQKSDGFAPLHLAAYNGRLDVVHILIKQGRSAVNARTCGGQTALMLAAGRGHVDVVFLLVREACKLDVEDEGGNTALHLVLKEADLFSTDCHLLQDDSTIVQIHYATRVLRINRLRDSGLFGAGSIYAGEALACFLVKEGANLRQTNARGKAPFD 768 HSP 2 Score: 56.225 bits (134), Expect = 2.114e-8 Identity = 86/390 (22.05%), Postives = 156/390 (40.00%), Query Frame = 3
Query: 327 IGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEI-FIVHPKTI----KSEIVENTELKXXXXXXXXXXXXXXLIT----DERTMRFLVALKTGNLSEVLRLVDSVGVNTNLKG----MYPLLIA---------ANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKA-DLNLQNIELRTALHIAV-KRKYLSFVKHLVENKAELDLKDKNGNTALHVA 1424
+G V+ D + +LQ HG + M I + +V + +GD + ++N +I +PK + ++ + + +L+ S I D +V G + V++++ S + L G P ++ A + ++L E+ + D + + A N + L+ +NAQ + T +A +G D+ L+ + A IGD +TALH A + + L++ A + N A HLAA G+ ++ + + VN+Q K + IH A N+E + L K DL QN T L +A K L+ + L ++ +D + +G LH+A
Sbjct: 241 LGDTVRCGVDGQTLMVLQQDHGGFSVAMALIAARPGKVAGLIDNGDVLVVYSNGGKYIYNPKCLIKVKMAQCISHLQLRQSFWIVIALIFSPSCIALINDDSINWMKMVKPTLGRVGRVVKVLPSGDLRVALGGHIWNFNPACVSTADPKSLDLAETSEDSAADILKERVILVWDTDLNKKQLISEAAQGNVGNVQKLVQLFPGQINAQQEGR-TALHVAAHQGHIDVARALLKA-GASTEIGDDKGNTALHYAAHGNEAAMAGLLVSHRARVNSRNHAGRTALHLAARKGYGEVARTLCEHACD--VNTQDSTKNSPIHDAIAENYEEVIEALTSVKTLDLERQNESGFTPLQLAAFKGSKLAVQRILAKSPKLVDEQKSDGFAPLHLA 626
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 119.783 bits (299), Expect = 4.583e-28 Identity = 97/350 (27.71%), Postives = 166/350 (47.43%), Query Frame = 3
Query: 711 LLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGF-----GNFLKMTSALGVLG-NIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELM 1742
L +AA+ GH +LL+K AN N+ +G T LH+A +NR ++ +LLLK GA + A +TP ++ G+ +I+ L++ A + + +TALH+A + + E V +LL GA + ++ H+AA G +I+++ L+ ++ + + +H+AA HE +L+++ A L + + T LH+A K + L++ A D KNG T LHVA + + G T+ G +I D+LE IA L+E GA + +G PL + L+ R + +H TK L + + + N AE++
Sbjct: 76 LHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIV-LILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASP--DAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLE-IATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEIL 421 HSP 2 Score: 108.997 bits (271), Expect = 1.009e-24 Identity = 73/248 (29.44%), Postives = 120/248 (48.39%), Query Frame = 3
Query: 699 GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQT 1442
G PL +AA EGHED+ +LLE A++ + K G T LH+A + ++ LLL++ A +A +TP +A +Q + LL+ A + T LH+A +LE LL GA+ + ++ HL A GH + + L R + VN + + +H+AA + + +L+K+ A +N T L +A + V L+++ A+++ K KNG T LH A + T
Sbjct: 237 GYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKV-ALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYAD--VNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHT 481 HSP 3 Score: 105.145 bits (261), Expect = 1.526e-23 Identity = 83/304 (27.30%), Postives = 139/304 (45.72%), Query Frame = 3
Query: 699 GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICN 1610
G+ PL AA GHE +V +LLE+ A I K+G + LH+A + + +L++ A V+ +T +A G ++L+ A+ ++ + T LH+A +R+ +E LL GA I + + H++A G+ I+ I L+ G N+ A+HMAA + L++ A ++ + E +T LHIA + L V+ L+++ A D NG T LHVA + + ED+ L+E+GAS+ + KG TPL + +
Sbjct: 6 GLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLL-DKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQ--GGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAR----------------------------EGHEDVTAILLEHGASLAVTTKKGFTPLHVAS 278 HSP 4 Score: 95.1301 bits (235), Expect = 1.892e-20 Identity = 81/317 (25.55%), Postives = 155/317 (48.90%), Query Frame = 3
Query: 699 GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTE------SGFGNFLKMT-SALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDI-CNDNSLK 1625
G+ P+ ++A G+ ++V +LL+ A+ N + G+TALH+A + ++ LL+ GA V+A+ ++ TP IA G +I++LL+ + A S+ T LH+A + LL GA + + KK F H+A+ G ++ + L+R ++ ++ +H+A+ +++ +L+ A + T LHIA K+ L L+E AE + + G LH+ + + + + + T S L L ++ DK+ ++A L+++GA ++ G TPL + C+ ++K
Sbjct: 138 GLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLL-QHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASP--DAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPL-HLAAQEDKV-NVAEILVKHGAHVNATTKLGYTPLIVACHYGNIK 449 HSP 5 Score: 91.2781 bits (225), Expect = 3.542e-19 Identity = 84/350 (24.00%), Postives = 155/350 (44.29%), Query Frame = 3
Query: 594 ITDERTMRFLVALKTGNLSEVLRLVDSVGVN--TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAM---KVNQTE--SGFGNFLKMTSALGV--------LGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPL 1598
+T+ V+ GNL+ VL L+ TN++G L +AA G ++V LL A ++ ++ T LH+A + ++ LLL+ A +A + TP +A +G +D+ +L+ + A L++ T LH+A ++E LL A K H+A+ ++K+ + L + ++ ++ + +H+AA + L++Y A+ N + LH+ + + L+ A+++ K+G + LH+A KVN E G + T+ LG GNI + +L+++GA ++ K G TPL
Sbjct: 134 VTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILL-EHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASP--HATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNI--------KMVNFLLQHGADVNSKTKNGYTPL 472 HSP 6 Score: 90.8929 bits (224), Expect = 3.927e-19 Identity = 64/231 (27.71%), Postives = 117/231 (50.65%), Query Frame = 3
Query: 669 DSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHL 1361
D+ G N G+ PL +A++ ++ + LLL++ A+ + K+G T LH+A +++ ++ LL+ GA+ NA + + P + +G D+ LL+ + AD++ + LHLA ++ E L+ GA + K + +A G+ K++ L+ + VNS+ ++ + +H AA H H + +L+K A N + TAL IA + Y+S V L
Sbjct: 297 DAAGKN----GLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLL-ARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGAD--VNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIARRLGYISVVDTL 520 HSP 7 Score: 77.7962 bits (190), Expect = 5.368e-15 Identity = 64/240 (26.67%), Postives = 108/240 (45.00%), Query Frame = 3
Query: 1113 HLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQ-----------------TESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKE------------------LLEHPVQPSDARSN 1727
H AA SGHE+++EI L++ L ++ ++ + +HMA +H C+ VLM+Y+A ++ ++ TALH+A + K L++ A +++ NG T LH+A K N+ TESG L +GN+ +I + L++ GAS + N +G+T L + + SL+ +Q ++E LL+H P A SN
Sbjct: 11 HCAARSGHEQVVEILLEQGAPIL--AKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESG----LTPMHVSAFMGNL--------NIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSN 236 HSP 8 Score: 48.521 bits (114), Expect = 5.461e-6 Identity = 30/81 (37.04%), Postives = 46/81 (56.79%), Query Frame = 3
Query: 642 NLSEVLRLVDSVGVNTNLK-GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVN 881
N++E+L + VN K G PL++A + G+ +VN LL+ A++N K+G T LH A + + +LLLK GA N
Sbjct: 416 NVAEIL-VKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPN 495
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 117.087 bits (292), Expect = 2.439e-27 Identity = 82/268 (30.60%), Postives = 134/268 (50.00%), Query Frame = 3
Query: 648 SEVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQT 1442
+E+++L+ G + N G PL A+ EGH D+ +LLLE A+I+ K G T LH+A R ++ LLL+K A + + +TP +A Q + LL+ + S + T LH+A ++LE + LL GA+ +L ++ HLAA GH ++ + L R ++ + A+H+AA + + +L K+KAD+N T LH+A + V L+ N+A ++ K +NG T LH A + T
Sbjct: 489 AEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGY-TPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHAS--ISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHT 753 HSP 2 Score: 114.39 bits (285), Expect = 1.446e-26 Identity = 85/302 (28.15%), Postives = 142/302 (47.02%), Query Frame = 3
Query: 702 MYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
+ PL +AA+ GH + LLL++ N +G T LH+A +NR K+ +LLLK GA ++ +TP +A G ++++LL+ N A + + +T LH+A + +LE V+ LL +GA + + ++ H+A+ G +I+++ L+R + N+ ++ A+ H +L++ AD++ + T LHIA K L L+E A D K KNG T LHVA + + +A+ L++ GAS G TPL I
Sbjct: 345 LTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLL-QNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDP--NAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDH----------------------------QKVALLLLDKGASPHSTAKNGYTPLHIA 615 HSP 3 Score: 107.457 bits (267), Expect = 2.125e-24 Identity = 87/344 (25.29%), Postives = 149/344 (43.31%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEK-------------------------------LVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTES-----GFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICN 1610
T G PL IAA+ G+ ++ LLL A ++ ++G T LH+A + LLL +GA ++ + +TP A G + ++E+L+ +A + + + LH+A L V+ LL A + + H+AA GH ++ ++ L R GE +++ E IH+A+ H + + +L++ A N N+ T LH+A + L VK L+++ A + +K + T LH+A ++ + E G+ +A G + D+A L+ENGA I KG TPL I +
Sbjct: 208 TTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLL-ERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIAS 550 HSP 4 Score: 101.679 bits (252), Expect = 1.476e-22 Identity = 82/279 (29.39%), Postives = 141/279 (50.54%), Query Frame = 3
Query: 618 FLVALKTGNLSEVL-RLVDSVGVNT-NLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRD------GEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK 1430
FL A + GNL ++ L + +NT N G+ L +A+ EGH D+V LL+++A I+ A K G+TALH+A + + +L++ GA++NAQ+ TP +A + +I++ L+ N A+ S+ D T L +A+ + V LL + K A H+AA + L+ + + ++N E F +H+AA + + +L+ A ++ T LH+A KR + VK L++ A++D K ++G T LH A +
Sbjct: 14 FLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLL-DNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTK----GKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAAR 287 HSP 5 Score: 100.908 bits (250), Expect = 2.575e-22 Identity = 65/221 (29.41%), Postives = 115/221 (52.04%), Query Frame = 3
Query: 699 GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHL 1361
G+ PL +A + H+ + LLL+K A+ + K+G T LH+A +N+ ++ +LL+ GA+ N + + P +A +G D++ LL+ +A +S+ TA+HLA ++ E L AD+ K + H+A+ G+ K++ L+ LVN++ + + +H AA H H + L++++A N TAL IA + Y+S V L
Sbjct: 575 GLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLL-GRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRN--RALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVVDTL 792 HSP 6 Score: 74.3294 bits (181), Expect = 4.638e-14 Identity = 50/140 (35.71%), Postives = 77/140 (55.00%), Query Frame = 3
Query: 651 EVLRLVDSVGVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALL 1061
EV +++ +G TN+ +G+ PL +AA EGH D+V LLL + A+I++A K G TA+HLA E++ + ++L K ADVN TP +A G+ ++ L+ N A ++ + T LH A V ALL
Sbjct: 622 EVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLL-RNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 760 HSP 7 Score: 66.6254 bits (161), Expect = 1.254e-11 Identity = 45/146 (30.82%), Postives = 78/146 (53.42%), Query Frame = 3
Query: 993 SDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK 1430
SD T+ A + LE + L G DI N+ NA HLA+ GH ++ LKR+ E +++ + A+H+AA+ + VL+++ A++N Q+ T L++A + +L VK L++N A + ++G T L VA++
Sbjct: 8 SDAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAE--IDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQ 151 HSP 8 Score: 51.9878 bits (123), Expect = 3.973e-7 Identity = 46/161 (28.57%), Postives = 73/161 (45.34%), Query Frame = 3
Query: 1194 KEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTE-----SGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQ 1661
K D + AA N + L +K D+N N ALH+A K ++ V L++ +AE+D K GNTALH+A QT+ FG + S G + +I +L++NGA+ + G TPL + + + L+E D++
Sbjct: 7 KSDAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTK 167
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 113.62 bits (283), Expect = 2.687e-26 Identity = 83/270 (30.74%), Postives = 133/270 (49.26%), Query Frame = 3
Query: 648 SEVLRLVDSVGVNTNLK---GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKK--GADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQT 1442
+E+++L+ G + N G PL A+ EGH D+ +LLLE A+I+ K G T LH+A R ++ LLL+K AD +TP +A Q + LL+ + S + T LH+A ++LE + LL GA+ +L ++ HLAA GH ++ + L R ++ + A+H+AA + + +L K+KAD+N T LH+A + V L+ N+A ++ K +NG T LH A + T
Sbjct: 482 AEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGY-TPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHAS--ISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHT 748 HSP 2 Score: 108.227 bits (269), Expect = 1.199e-24 Identity = 84/304 (27.63%), Postives = 142/304 (46.71%), Query Frame = 3
Query: 702 MYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLK--DKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDIC 1607
+ PL +AA+ GH + LLL++ N +G T LH+A +NR K+ +LLLK GA ++ +TP +A G ++++LL+ N A + + +T LH+A + +LE V+ LL +GA + + ++ H+A+ G +I+++ L+R + N+ ++ A+ H +L++ AD++ + T LHIA K L L+E A D K +NG T LHVA + + +A+ L++ GAS G TPL I
Sbjct: 338 LTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLL-QNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDP--NAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDH----------------------------QKVALLLLDKGASPHSTAKNGYTPLHIA 610 HSP 3 Score: 108.227 bits (269), Expect = 1.379e-24 Identity = 87/344 (25.29%), Postives = 150/344 (43.60%), Query Frame = 3
Query: 687 TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEK-------------------------------LVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTES-----GFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICN 1610
T+ G PL IAA+ G+ ++ LLL A ++ ++G T LH+A + LLL +GA ++ + +TP A G + ++E+L+ +A + + + LH+A L V+ LL A + + H+AA GH ++ ++ L R GE +++ E IH+A+ H + + +L++ A N N+ T LH+A + L VK L+++ A + +K + T LH+A ++ + E G+ +A G + D+A L+ENGA I KG TPL I +
Sbjct: 201 TSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLL-ERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIAS 543 HSP 4 Score: 103.605 bits (257), Expect = 3.218e-23 Identity = 93/349 (26.65%), Postives = 160/349 (45.85%), Query Frame = 3
Query: 618 FLVALKTGNLSEVL-RLVDSVGVNT-NLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDS 1658
FL A + GNL ++ L + +NT N G+ L +A+ EGH D+V LL+++A I+ A K G+TALH+A + + +L++ GA++NAQ+ TP L++A + LE V+ LL+ GA+ + + F +A GHE+++ + L+ D ++ + + A+H+AA + + +L++++ + ++ + T LHIA ++ L+ A +D +NG T LHVA K G N +K+ L++ GA ID K G TPL + + I L+ERD+
Sbjct: 15 FLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTP----------------------------------LYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESD------TKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAK-----RGNNNMVKL-----------------------LLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDA 295 HSP 5 Score: 100.908 bits (250), Expect = 2.491e-22 Identity = 64/218 (29.36%), Postives = 114/218 (52.29%), Query Frame = 3
Query: 699 GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFV 1352
G+ PL +A + H+ + LLL+K A+ + K+G T LH+A +N+ ++ +LL+ GA+ N + + P +A +G D++ LL+ +A +S+ TA+HLA ++ E L AD+ K + H+A+ G+ K++ L+ LVN++ + + +H AA H H + L++++A N TAL IA + Y+S V
Sbjct: 570 GLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLL-GRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRN--RALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVV 784 HSP 6 Score: 74.7146 bits (182), Expect = 3.579e-14 Identity = 50/140 (35.71%), Postives = 77/140 (55.00%), Query Frame = 3
Query: 651 EVLRLVDSVGVNTNL---KGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALL 1061
EV +++ +G TN+ +G+ PL +AA EGH D+V LLL + A+I++A K G TA+HLA E++ + ++L K ADVN TP +A G+ ++ L+ N A ++ + T LH A V ALL
Sbjct: 617 EVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLL-RNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 755 HSP 7 Score: 66.2402 bits (160), Expect = 1.510e-11 Identity = 45/146 (30.82%), Postives = 78/146 (53.42%), Query Frame = 3
Query: 993 SDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMK 1430
SD T+ A + LE + L G DI N+ NA HLA+ GH ++ LKR+ E +++ + A+H+AA+ + VL+++ A++N Q+ T L++A + +L VK L++N A + ++G T L VA++
Sbjct: 9 SDAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAE--IDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQ 152 HSP 8 Score: 51.6026 bits (122), Expect = 5.210e-7 Identity = 40/138 (28.99%), Postives = 65/138 (47.10%), Query Frame = 3
Query: 1263 MKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTE-----SGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQ 1661
+K D+N N ALH+A K ++ V L++ +AE+D K GNTALH+A QT+ FG + S G + +I +L++NGA+ + G TPL + + + L+E D++
Sbjct: 31 LKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTK 168
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Sea Lamprey
Match: ankdd1a (ankyrin repeat and death domain containing 1A [Source:ZFIN;Acc:ZDB-GENE-070615-8]) HSP 1 Score: 110.538 bits (275), Expect = 7.940e-26 Identity = 95/319 (29.78%), Postives = 148/319 (46.39%), Query Frame = 3
Query: 618 FLVALKTGNLSEVLRLVDSVGVNT-NLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQN-----------------------------------NKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYL--LNKKLFN---AYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESG 1451
F A K ++ E+ +L+ V V+ N++ L AA G ED V LLLE+KAN++ DK G L +A ++ L+ G V++QN N + TP ++A G +LELL+ A + D +++TA+HLA S + S+E LL G L +N + N A HLAA GH + + + L + +N+ + A+HMAA + HE +L+ D+N + + + LH+AV+ Y V LVE +A ++L D TALH+A + ES
Sbjct: 7 FHEAAKRNDVDEMEKLLKKVKVDAKNMRDQTALHFAAGSGQEDAVKLLLERKANVDKGDKYGMNPLLIAAWFGHLEVLKRLVNGGGRVSSQNKNGLNILHCAAQRGHVETVKYTLESLEDLGIEGKTNAEKTPYLLAAEYGQLAVLELLMDYKCAQ-DVSDGEKNTAIHLAAKSGHMLSLEHLLEKGPGRTLDFVNSQNANGNTALHLAAEGGHLECVRMLLDCEAICDINALNNESMNALHMAAKHGHEQIAKLLIDETIDINAISTKQASPLHLAVQNNYPIIVTFLVEAEANINLVDCRNQTALHIAAETGFQESA 324 HSP 2 Score: 70.8626 bits (172), Expect = 3.897e-13 Identity = 63/225 (28.00%), Postives = 114/225 (50.67%), Query Frame = 3
Query: 660 RLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKA--NINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRD----GEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTAL 1316
R +D V + N G L +AA GH + V +LL+ +A +IN + + ALH+A ++ LL+ + D+NA + K+ +P +AV I+ L+ + A++++ D TALH+A + ES + LL AG D+ LL+K+ + +AA + ++++ +K D K+ N+++E+ NN E L + ++ D + LR+ L
Sbjct: 195 RTLDFVN-SQNANGNTALHLAAEGGHLECVRMLLDCEAICDINALNNESMNALHMAAKHGHEQIAKLLIDETIDINAISTKQASPLHLAVQNNYPIIVTFLVEAE-ANINLVDCRNQTALHIAAETGFQESADLLLAAGTDLTLLDKQKKSCLEVAARANQVNLVDMIIKADRYFTRRKVRNAKQEEG---------NNEESVLGAVT-FRPDRRAETQHLRSVL 407 HSP 3 Score: 67.3958 bits (163), Expect = 5.439e-12 Identity = 79/300 (26.33%), Postives = 132/300 (44.00%), Query Frame = 3
Query: 810 LHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDL-----KDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIG----EGLDKL--------EDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDS 1658
H A N + LLKK V+A+N + T A G +D ++LL+ A++ GD L +A LE ++ L+N G + NK N H AA GH + ++ L+ + + + + +AA L +LM YK ++ + E TA+H+A K ++ ++HL+E L ++ NGNTALH+A E G ++M + +I E ++ L E IA LI+ I+ + K +PL + N+ + LVE ++
Sbjct: 7 FHEAAKRNDVDEMEKLLKK-VKVDAKNMRDQTALHFAAGSGQEDAVKLLL-ERKANVDKGDKYGMNPLLIAAWFGHLEVLKRLVNGGGRVSSQNKNGLNILHCAAQRGHVETVKYTLESLEDLGIEGKTNAEKTPYLLAAEYGQLAVLELLMDYKCAQDVSDGEKNTAIHLAAKSGHMLSLEHLLEKGPGRTLDFVNSQNANGNTALHLA-----AEGGHLECVRMLLDCEAICDINALNNESMNALHMAAKHGHEQIAKLLIDETIDINAISTKQASPLHLAVQNNYPIIVTFLVEAEA 299 HSP 4 Score: 56.9954 bits (136), Expect = 1.019e-8 Identity = 46/166 (27.71%), Postives = 84/166 (50.60%), Query Frame = 3
Query: 624 VALKTGNLSEVLRLVDSVGV----NTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNA 1109
+A + G+L V L+D + N + M L +AA GHE + LL+++ +IN + LHLAV N + L++ A++N + + T IA G Q+ +LL+ + DL++ D + + L +A ++++ V+ ++ AD Y +K+ NA
Sbjct: 213 LAAEGGHLECVRMLLDCEAICDINALNNESMNALHMAAKHGHEQIAKLLIDETIDINAISTKQASPLHLAVQNNYPIIVTFLVEAEANINLVDCRNQTALHIAAETGFQESADLLLAAGT-DLTLLDKQKKSCLEVAARANQVNLVDMIIK--ADRYFTRRKVRNA 375
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Yeast
Match: AVO2 (Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth [Source:SGD;Acc:S000004672]) HSP 1 Score: 61.6178 bits (148), Expect = 1.548e-10 Identity = 50/162 (30.86%), Postives = 87/162 (53.70%), Query Frame = 3
Query: 918 AVLKGSQDILELLITSNNADLSIGDSDRD-TALHLAVNSSRLESVEALLNAGADIYLLNK--KLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKD 1394
A+++G+ I++ L+ N L+ DS+ ++LH A R L+ G D + L K K HLA + GHE+ L + L++ + +N + E+ A IH+A MN++ CL +L+ ADL + + T LH+ ++ +S +K L+ N+ E+ L D
Sbjct: 12 AIIEGNLLIVKRLLRRNPDLLTNIDSENGWSSLHYASYHGRYLICVYLIQLGHDKHELIKTFKGNTCVHLALMKGHEQTLHLLLQQ-FPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKMLL-NEGEVSLDD 171
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Yeast
Match: AKR2 (Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication [Source:SGD;Acc:S000005560]) HSP 1 Score: 57.7658 bits (138), Expect = 4.493e-9 Identity = 57/210 (27.14%), Postives = 96/210 (45.71%), Query Frame = 3
Query: 618 FLVALKTGNLSEVLRLVDSVGVNTN------LKGMYPLLIAANEGHEDLVNLLLEKKANIN-IADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLIT-----SNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKIL-EIFLKRDGEKLVNSQKEDKF 1208
F+ A K G+L V +V+S V+ N L G++ A + LL + AN N A G TALH A + DLLLK GAD ++ + + +V + ++ ++ ++N D+ D++ T L A +VE LL G+ + + + FNA H A + G ++++ ++ L N+QK+D F
Sbjct: 54 FIEAAKDGDLKVVKDVVESGAVDINNDRIDELSGLH---WACINNRFSVAKFLLLRGANPNQAAGPGGATALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFSVYSSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAYQGDFLTVELLLKFGSTVAWTDNRGFNALHCALVGGDQRVICDLILSGANFYERNNQKQDCF 260
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672]) HSP 1 Score: 56.225 bits (134), Expect = 1.105e-8 Identity = 57/227 (25.11%), Postives = 94/227 (41.41%), Query Frame = 3
Query: 786 ADKDGD-----TALHLAVHENRFKLCDLLLKKGADVNAQNNK-KITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTES 1448
+ DGD T LH A NR + D L+ +GADVNA+ TP A G I++ L+ + AD ++ D LHL+VNSS +I L+ LFN + +L+I + + ++ AA + L+K+ A + + + E T LH + +K+L+++ A+ K G +A ++N S
Sbjct: 100 VNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLL-KHGADPTMTDDQGFNLLHLSVNSS-------------NIMLVLYVLFNVV-------SKGLLDI----------DCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKDCFAIAQEMNTVYS 295
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374]) HSP 1 Score: 48.521 bits (114), Expect = 3.478e-6 Identity = 35/106 (33.02%), Postives = 60/106 (56.60%), Query Frame = 3
Query: 678 GVNTNLKGMYPLLIAANEGHEDLVNLLLEKKA-NINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKI--TPTMIAVLKGSQDILELLITSNNADLSI 986
G+ + G L IA ++G D+V ++E+ +IN D G+TALH A + ++ +LL++ GADVN ++ + TP + A G D+++ L+ N AD +I
Sbjct: 323 GIYRDSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLL-KNGADPTI 427
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Nematostella
Match: EDO39113 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB41]) HSP 1 Score: 75.485 bits (184), Expect = 8.389e-16 Identity = 50/161 (31.06%), Postives = 80/161 (49.69%), Query Frame = 3
Query: 699 GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALH------LAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLK 1163
G PL A H D+V +LL+ A++++ G ++ A N + C +LL+ GADVN N T A +G ++ELL+ D+++ D D T+LH+A ++ LL GA+I + F A+HLAA G E++L+ L+
Sbjct: 29 GFSPLHYATTTNHVDIVQMLLDAGADVDVR---GSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEVLQTLLR 186 HSP 2 Score: 68.9366 bits (167), Expect = 1.759e-13 Identity = 54/188 (28.72%), Postives = 91/188 (48.40%), Query Frame = 3
Query: 720 AANEGHEDLVNLLLEKK-ANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNN---KKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGA-DIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMK 1268
A +G +D V LL N+N D DG + LH A N + +LL GADV+ + + ++I P + A + + +L+ + AD++I + TALH A + VE LL G D+ ++++ + H+AA+ G I++ L + ++ D F A H+AA E L L++
Sbjct: 2 ACRDGRKDCVRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRA-GADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGAN--ITAKDNDGFTAFHLAAREGEEEVLQTLLR 186 HSP 3 Score: 61.6178 bits (148), Expect = 5.058e-11 Identity = 44/152 (28.95%), Postives = 77/152 (50.66%), Query Frame = 3
Query: 636 TGNLSEVLRLVDSVGVNTNLKG------MYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGA-DVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAG 1070
T N ++++++ G + +++G + P++ AA + +LL A++NI + G TALH A K+ +LLL+ G DVN + T +A + GS I++ L+ A+++ D+D TA HLA E ++ LL G
Sbjct: 38 TTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCG-ANITAKDNDGFTAFHLAAREGEEEVLQTLLRTG 188 HSP 4 Score: 50.0618 bits (118), Expect = 4.949e-7 Identity = 44/174 (25.29%), Postives = 76/174 (43.68%), Query Frame = 3
Query: 918 AVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADI------------YLLNKKLFN------------------------AYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVK 1331
A G +D + L+ N +L+ D+D + LH A ++ ++ V+ LL+AGAD+ ++ FN A H A GH K++E+ L RDG+ VN D ++H+AAM + L+ A++ ++ + TA H+A +
Sbjct: 2 ACRDGRKDCVRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLL-RDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAR 174
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Nematostella
Match: EDO49485 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG84]) HSP 1 Score: 73.559 bits (179), Expect = 8.830e-15 Identity = 64/211 (30.33%), Postives = 104/211 (49.29%), Query Frame = 3
Query: 810 LHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHE-KILEIFLKRDGEKLVNSQKEDK--FAAIHMAAMNNHEHCLPVLMKYKAD--LNLQN-IELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVA 1424
LHLA + C LL G DVNA++N TP A G+ + LELL+ S D + D LH A + V +L+ AG+++ + K H AA S + K +E L+ G N+ K DK ++A+H A+ N H+ + +L+ A+ LN + T LH+A + ++ L+++ +L+++D G T L +A
Sbjct: 4 LHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLM-SQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRG----NASKRDKQGYSAVHYASANGHKLAIEMLLDVSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLA 209
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Nematostella
Match: EDO38725 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC11]) HSP 1 Score: 68.5514 bits (166), Expect = 4.428e-13 Identity = 49/174 (28.16%), Postives = 87/174 (50.00%), Query Frame = 3
Query: 708 PLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAA 1229
PL +A +G ++ LL+EK A ++ + G+T LH+A F LL KG ++ ++ TP + AV G + LL+ D ++ + LHLA + R VEAL AG +++ +A H+AA+SG K++ L+ + +N++ +++ + AA
Sbjct: 39 PLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAGRTENAMLLLEK--------DENQKSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGAD--INARDSNRWTPLMCAA 202 HSP 2 Score: 50.8322 bits (120), Expect = 4.002e-7 Identity = 53/226 (23.45%), Postives = 100/226 (44.25%), Query Frame = 3
Query: 777 INIADKDGDTALHLAVHENRFKLCDLLLKKGA--DVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTES 1448
+N D G +ALH A NR + L++ G+ D++ TP +A KGS ++ LL+ A L + +T LH+A ++++ LL+ G I + + A +G + + L++D E++ + +H+A + L + N ++ E ++ALH+A + V L+ A+++ +D N T L A K ++
Sbjct: 1 LNKMDDTGFSALHHATRYNRVEAMAELIRNGSIEDLS-------TPLHVACKKGSMEVGRLLV-EKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAGRTENAMLLLEKD---------ENQKSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWTPLMCAAKTGSVKT 209 HSP 3 Score: 49.6766 bits (117), Expect = 1.150e-6 Identity = 37/114 (32.46%), Postives = 57/114 (50.00%), Query Frame = 3
Query: 618 FLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGS-QDILELL 956
FL A+ G + L++ + N K PL +A G V L + N D++G +ALH+A K+ LL+ GAD+NA+++ + TP M A GS + +ELL
Sbjct: 106 FLSAVAAGRTENAMLLLEK---DENQKS--PLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWTPLMCAAKTGSVKTCMELL 214
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57]) HSP 1 Score: 70.0922 bits (170), Expect = 1.243e-12 Identity = 64/241 (26.56%), Postives = 104/241 (43.15%), Query Frame = 3
Query: 744 LVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSS-------RLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTE 1445
++ ++ +A I DKD DT L +A +N LL+ GAD+ A++ A +G + LE + +I D D L+ V S R+ S + LL+ GA I N + H AA G + + LKRD ++N + +H+AA++ H + L+ ++ +N L T L A R + L++ A +D DK T LH+A + E
Sbjct: 162 IICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICD---DVCLNGLVGGSNKLILVLRISS-QLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRD-PAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVE 397 HSP 2 Score: 63.1586 bits (152), Expect = 1.815e-10 Identity = 62/248 (25.00%), Postives = 101/248 (40.73%), Query Frame = 3
Query: 708 PLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHEN--------------RFKLCD-----------------------LLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKY 1340
PL+IAA + H D V LL+ A+I D + ++ A E + +CD LLL GA ++++N++ ITP A G + L+ + A ++ D +T LHLA V+ LL G I N L+ AA G ++ I L D V+ + K +H+A+ H + +L+ KA++ L + R L IA++ +
Sbjct: 183 PLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLILVLRISSQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAP--VDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSH 428 HSP 3 Score: 60.4622 bits (145), Expect = 1.141e-9 Identity = 63/303 (20.79%), Postives = 134/303 (44.22%), Query Frame = 3
Query: 804 TALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITS-------NNADLSIGDSDRD--TALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTES---------GFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDS 1658
T LH+A + L++ GAD+ + + +++TP A ++G+ + +LL + + + D DR+ TALHLAV + + L GA++ + + +H + E I + +K K ++ K +K + + + +++++A + ++ + T L IA ++ ++ V+ L+++ A++ KD N ++ A E+ G++G + + L + L++NGA ID KN++ TP+ + +L++RD
Sbjct: 6 TPLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVAAELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIVVARECIF-VMIKVSKLKYLSFCKSEKIKREILLYFFDVVIIICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLILVLRISSQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDP 307
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Nematostella
Match: EDO43690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXV8]) HSP 1 Score: 67.3958 bits (163), Expect = 3.216e-12 Identity = 61/228 (26.75%), Postives = 115/228 (50.44%), Query Frame = 3
Query: 618 FLVALKTGNLSEVLRLV-DSVGVN-TNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQN 1295
FL A+ +G++ EV +L+ +N N+ G+ L A + + DLV +L++K A++++ D +G T+LH A ++ L++ GADV A NN+ P +A + +D+L I + D+ D R + +N ++ + +N D K +A H+A+ G+ K++ + L+ E +N++ D + +H AA + +L + AD N +N
Sbjct: 53 FLSAVSSGDVDEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDV---DDARAVEEKIMLNDAQSWLNKNCINESKD-----KTEASALHVASSKGYLKVIGLLLQLGVE--INAKDADNWTPLHAAAHWGQKDACELLSENGADFNARN 270 HSP 2 Score: 63.1586 bits (152), Expect = 7.663e-11 Identity = 45/155 (29.03%), Postives = 70/155 (45.16%), Query Frame = 3
Query: 1260 LMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARS 1724
L+ AD+N QN++ TALH A + VK LV+ A +D++D G T+LH A E IA YLIENGA + NN+G+ PLD+ + ++ + +E +Q + +E + +DA+S
Sbjct: 68 LLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVE----------------------------IAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIE--NQGIDVDDARAVEEKIMLNDAQS 192 HSP 3 Score: 50.8322 bits (120), Expect = 6.712e-7 Identity = 49/198 (24.75%), Postives = 97/198 (48.99%), Query Frame = 3
Query: 855 LLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTES 1448
LL KGAD+N QN +T A + + D++++L+ A + + D++ T+LH A + +E + L+ GAD+ +N + LA E +L ++ G + +++ ++ ++ A +++C+ K K + +ALH+A + YL + L++ E++ KD + T LH A Q ++
Sbjct: 68 LLAKGADINYQNVDGLTALHQACIDENFDLVKVLV-DKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDARAVEEKIMLNDAQSWLNKNCINE-SKDKTEA--------SALHVASSKGYLKVIGLLLQLGVEINAKDADNWTPLHAAAHWGQKDA 255
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Medaka
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:101158637]) HSP 1 Score: 259.996 bits (663), Expect = 1.605e-74 Identity = 189/630 (30.00%), Postives = 304/630 (48.25%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVK-------------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEV---LRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGV-------------LGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ + +G VQ+ D + KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G+L++V L+ D V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A++ + T S M V LG G IA +L NGA + I+N KG++PLD+C D +L + L+ ++ + + PS L ++ +E+ C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 258 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKIAPSGSAVSNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPD-VDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALR-HHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPNL-CKALAKCHKEKSSGQVGTRS-------PS-----------LNSNMESLEE---CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 862 HSP 2 Score: 56.6102 bits (135), Expect = 7.353e-8 Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3
Query: 1731 AELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
A++ +L+Q+L++++++ +C +C DR +IF CGH C C D +SEC +C + I +I
Sbjct: 959 ADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRI 1017
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Medaka
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:101158637]) HSP 1 Score: 260.381 bits (664), Expect = 2.136e-74 Identity = 189/630 (30.00%), Postives = 304/630 (48.25%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKLIN-DDGSDATVMGPSGMIYRLNSELLVPFKSVK-------------KHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDER---TMRFLVALKTGNLSEV---LRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLM-KYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGV-------------LGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGHMC-CDDCSDNISECHLCSEKILHKIKI 1913
DL + Q HGGW D M E LT + D+ D V PSG + N +L V+ + +G VQ+ D + KLLQ HGEW E M LGK V QI+ D D K+ + +P + + + + + + T E + A G+L++V L+ D V VN G + A+ GH D++ LLL+ ++ DKDGD A+H A + + ++L + GAD+NA+N ++ TP IAV KG +++ L+ S+ DS+ DT LH A++ R + + LL AGAD+ + N FNA H AA+ G+ + + L + +V+ +K+D + A+H+AA+NNH +L+ + A L++QN+ +TALH+AV+R++ V+ LV +A+LD++DK+G+T LH A++ + T S M V LG G IA +L NGA + I+N KG++PLD+C D +L + L+ ++ + + PS L ++ +E+ C +C D + +F CGH+ C CS + +C +C E++ + KI
Sbjct: 258 DLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKIAPSGSAVSNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPD-VDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLL-DFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALR-HHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPNL-CKALAKCHKEKSSGQVGTRS-------PS-----------LNSNMESLEE---CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 862
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479]) HSP 1 Score: 238.81 bits (608), Expect = 2.146e-67 Identity = 188/623 (30.18%), Postives = 308/623 (49.44%), Query Frame = 3
Query: 159 QKNHGGWNDLMKE--CLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIV---------ENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLK--GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGA-DIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQ--------------TESGFGNFLKMT-----SALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDA---RSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGH-MCCDDCSDNISECHLCSEKILHKIK 1910
Q+ HGGWN M E C G I D G D V + + + + L V +G V+++ + E K LQ HGEW + M +LG+ +V++++ DGD +++ + + +P + ++ V EN + + R ++ G+ +V LV ++K G L +AA++GH ++V LL+ ++ + D+DGDTALH A N+ ++ LLL KGA+VN NN T IAV KG D++ +L T ++AD+++ DS DT LH A+ +E L+ + D N + FN H AA+ G++ E L R +LV+ +K+D F+A+H+AA+NNH VL+K + D+N++N +TAL +AV + + V+ LV+ A+++++D++G+TA+HVA+ Q TE T S+ G+LGN GE L IA +L + GA I+ N+KGK+PLD+ D+ L L++ S+KH + + + + S A R + L C IC + L V+F C H + C++C+ + +C C I KI+
Sbjct: 268 QEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALT---KVNTFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVISVLEKLLSQSTEQDNPCRLVIEAAHGSAVKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLRPQLANVMLSPAVGTGATEDNSEGCSPTTLHGRLSSSGLLGN-GE-LSVGTAIACFLAQEGADINYANHKGKSPLDLLADSG----ALQLIKSFSEKHRLQRLQAITCGQGLSSASLRRVHTTPNTMTNLVLPTPPGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIR 878
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479]) HSP 1 Score: 239.58 bits (610), Expect = 2.672e-67 Identity = 188/623 (30.18%), Postives = 308/623 (49.44%), Query Frame = 3
Query: 159 QKNHGGWNDLMKE--CLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIV---------ENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLK--GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGA-DIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQ--------------TESGFGNFLKMT-----SALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDA---RSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGH-MCCDDCSDNISECHLCSEKILHKIK 1910
Q+ HGGWN M E C G I D G D V + + + + L V +G V+++ + E K LQ HGEW + M +LG+ +V++++ DGD +++ + + +P + ++ V EN + + R ++ G+ +V LV ++K G L +AA++GH ++V LL+ ++ + D+DGDTALH A N+ ++ LLL KGA+VN NN T IAV KG D++ +L T ++AD+++ DS DT LH A+ +E L+ + D N + FN H AA+ G++ E L R +LV+ +K+D F+A+H+AA+NNH VL+K + D+N++N +TAL +AV + + V+ LV+ A+++++D++G+TA+HVA+ Q TE T S+ G+LGN GE L IA +L + GA I+ N+KGK+PLD+ D+ L L++ S+KH + + + + S A R + L C IC + L V+F C H + C++C+ + +C C I KI+
Sbjct: 337 QEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALT---KVNTFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVISVLEKLLSQSTEQDNPCRLVIEAAHGSAVKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLRPQLANVMLSPAVGTGATEDNSEGCSPTTLHGRLSSSGLLGN-GE-LSVGTAIACFLAQEGADINYANHKGKSPLDLLADSG----ALQLIKSFSEKHRLQRLQAITCGQGLSSASLRRVHTTPNTMTNLVLPTPPGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIR 947
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479]) HSP 1 Score: 239.58 bits (610), Expect = 3.421e-67 Identity = 188/623 (30.18%), Postives = 308/623 (49.44%), Query Frame = 3
Query: 159 QKNHGGWNDLMKE--CLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIV---------ENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLK--GMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGA-DIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQ--------------TESGFGNFLKMT-----SALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDA---RSNEAELMELRQKLKEMEDEKICKICHDRRLKVIF-ECGH-MCCDDCSDNISECHLCSEKILHKIK 1910
Q+ HGGWN M E C G I D G D V + + + + L V +G V+++ + E K LQ HGEW + M +LG+ +V++++ DGD +++ + + +P + ++ V EN + + R ++ G+ +V LV ++K G L +AA++GH ++V LL+ ++ + D+DGDTALH A N+ ++ LLL KGA+VN NN T IAV KG D++ +L T ++AD+++ DS DT LH A+ +E L+ + D N + FN H AA+ G++ E L R +LV+ +K+D F+A+H+AA+NNH VL+K + D+N++N +TAL +AV + + V+ LV+ A+++++D++G+TA+HVA+ Q TE T S+ G+LGN GE L IA +L + GA I+ N+KGK+PLD+ D+ L L++ S+KH + + + + S A R + L C IC + L V+F C H + C++C+ + +C C I KI+
Sbjct: 393 QEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALT---KVNTFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVISVLEKLLSQSTEQDNPCRLVIEAAHGSAVKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLRPQLANVMLSPAVGTGATEDNSEGCSPTTLHGRLSSSGLLGN-GE-LSVGTAIACFLAQEGADINYANHKGKSPLDLLADSG----ALQLIKSFSEKHRLQRLQAITCGQGLSSASLRRVHTTPNTMTNLVLPTPPGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIR 1003
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Planmine SMEST
Match: SMESG000031930.1 (SMESG000031930.1) HSP 1 Score: 1206.82 bits (3121), Expect = 0.000e+0 Identity = 613/613 (100.00%), Postives = 613/613 (100.00%), Query Frame = 3
Query: 78 MGANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKIN 1916
MGANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKSDDSDDSDDSNDRSLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKIN
Sbjct: 1 MGANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKSDDSDDSDDSNDRSLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITKKELLEHPVQPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKIN 613
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Planmine SMEST
Match: SMESG000017281.1 (SMESG000017281.1) HSP 1 Score: 553.132 bits (1424), Expect = 0.000e+0 Identity = 309/641 (48.21%), Postives = 411/641 (64.12%), Query Frame = 3
Query: 117 VVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLIN---DDGSDATVMGPSGMIYRLNSELLVPF--KSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITD--------------------ERTMRFLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTES--GFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHI--------TKKELLEHPV-------QPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
VI + D+ + Q+NHGGW M G C I G + G++Y N LL +K G V+LI D+ + KLLQ HGEW M LGK+ +V +F DGD ++ N+ ++P+ +++ ++ S S +L +D E M+ A+K G L+ V L +SVGVN LKG+YPL+IA NEGH D+V LL+EK+AN+N DKDGDTALHLAV +N+ K+CDLL+ KGADVNAQNN KITP M+A GS +I LL TS NADLSIGDSD DTALHLAV SR+ SVE LL+AGADI LNKKLFN +HLAA+ GH KILE+FLKR+G K+V+SQKEDKFAAIH+ A+NNHE CL VL+ YKAD+N++N L+TALHIAV +K+L+ VK LVEN AE++L+D +G+T LH+AMKV Q E+ G M S +G+LG++ EG D +DIAIYL+++GA +D+KN KG TP+++CND+ LK RILSL +S K + TK E E P+ P + +S + EL ELRQ++++MEDEK C IC+DR + V F CGH+ CD C++N+ ECH+C E I +KI++
Sbjct: 240 TVILSDDIQKTKQLQQNHGGWVSRM-SVFAGKCGKIEAILPTGDVKVNVNGLGLVY--NPLLLTKSDKSDLKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNLTLNPECVEATDSDSDSDPDIFSMMEHLSKLMNLDSDQSDQEDKGKDSDDGKTDGNQEEVMKLFFAVKNGELNNVRNLCESVGVNARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENYAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLNIPNSTKQDLIEKEPNPGTKAERFEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRL 877 HSP 2 Score: 163.696 bits (413), Expect = 6.320e-42 Identity = 80/151 (52.98%), Postives = 103/151 (68.21%), Query Frame = 3
Query: 78 MGANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHK--IGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSE 524
MGA++S VVIA +DL TII+ Q HGGWND M ECL GPCKL+ GSDA V GPSG +YRLNSELL + S + G V+LIQDE++F+ LQ HGEW +M++I+GK+V+VI F DGD KI +N F+++PK I S+
Sbjct: 1 MGASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNNNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 151 HSP 3 Score: 64.6994 bits (156), Expect = 1.898e-10 Identity = 47/161 (29.19%), Postives = 77/161 (47.83%), Query Frame = 3
Query: 54 FNTEYYY*MGANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLV------PFKSV-KKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTI 515
N+E G++ S I VV QD + Q HG WN+ MK+ + ++I+ + +G + +N + + P V K KIG V LI + + K LQ HG W + + GKEVE+++IF+DGD +S ++++P+ I
Sbjct: 68 LNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNNNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGEKVVLIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 228
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Planmine SMEST
Match: SMESG000017279.1 (SMESG000017279.1) HSP 1 Score: 480.715 bits (1236), Expect = 1.053e-158 Identity = 281/644 (43.63%), Postives = 394/644 (61.18%), Query Frame = 3
Query: 135 DLSTIIKKQKNHGGWNDLMKECLTGPCKL----------INDD------------GSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKS-----EIVENTELKXXXXXXXXXXXXXXLITDE----RTMRFLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTES--GFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITK------------KELLEHPVQPSDA------RSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 1913
D+ I + Q+NHGGW + M+ C CK+ +N D + P+ + + VK G V+LI ++ + K LQ HGEW M+ +LGK +V + +GD K+ + ++P+ +++ E + + + +D S +DE RT F ALK GNL+EV +LVDSVG +T L+G+YPL+IA NEGH D++ LL+EK+ANIN +++GDTALH+AV +++FK+CDLL+ KGADVNAQNN KITP M+A GS DI LL TS NADLSIG+S+ DTALHLAV + ESV LL+AGADI +N K FNA+HL A G+E+ILE+FLKR+G K+VNSQK+DKFAA+H+A NNHE CL VL+KYKAD+N++N L+TALHIAV +KYL+ +K LVE AE+D+ D NG+TALH+A++++ ++ G L ++ + EG DK ED+AIYL++ A DIKN +TP+D C D SLK ++ R K TK L+ HP D ++ + EL ELRQ++++MEDE+ C IC DR+ V F CGH+ CD C++N+ ECH+C E I +KI++
Sbjct: 250 DIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARAQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNEGHIDVIKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNVLLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTALHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDRTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGSTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDERTCIICFDRQNNVTFGCGHVACDVCANNLYECHICRENIGNKIRL 892 HSP 2 Score: 162.54 bits (410), Expect = 2.097e-41 Identity = 76/144 (52.78%), Postives = 102/144 (70.83%), Query Frame = 3
Query: 114 KVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTEL 545
+VVI +D+ TI KQ+ HGGWN M ECLTGPC+L+ GS A V GPSG +YRLNSELL+ + + + HKIGSFV LI++ EEFK LQ +GEW +DM NILGK+VE+IQ F+DG+ KI + F ++ K I ++ + T +
Sbjct: 13 QVVIGTKDMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTSM 156 HSP 3 Score: 61.2326 bits (147), Expect = 2.374e-9 Identity = 100/442 (22.62%), Postives = 183/442 (41.40%), Query Frame = 3
Query: 159 QKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVP-----FKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVN---LLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSI-GDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHL--AAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENK-AELDLKDKNGNTALHVAMKVNQTES 1448
QK+HGGW +LM + +++ + GM + +N + + FKS K K G VQ+ D ++ K LQ HG W M GK +V I GD K++ + + +P + E + T R R ++ L ++ V S G L + +GH + VN +L K + D +GD L + + + L + + D + ++ I TM+ + +++ S+++D S D+DR A+ L V L+++ LL Y L A GH ++++ +++ +N+++E+ A+H+A + +L+ AD+N QN T + +A + + L ++ A+L + + +TALH+A+K ES
Sbjct: 181 QKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRK-GDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTK--CEGNQANP---------------TAARAQR----RRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGD--LKVKIMGQKLNLNPECV-EATDSDCESEPDIQ-TMV------NQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLE----GVYPLMIAVNEGHIDVIKLLIEKRAN--INAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAES 584 HSP 4 Score: 55.4546 bits (132), Expect = 1.446e-7 Identity = 38/127 (29.92%), Postives = 62/127 (48.82%), Query Frame = 3
Query: 159 QKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSF--------VQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTI 515
Q +G W+D M L ++I + G +R+NS+ ++ + KK + V LI++ E K LQ HG W E M +G+EVE+++I KDGD +S ++++PK I
Sbjct: 101 QVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT-DNCKKTSMEMEETFEEGEKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCI 226
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Planmine SMEST
Match: SMESG000030607.1 (SMESG000030607.1) HSP 1 Score: 187.963 bits (476), Expect = 1.276e-49 Identity = 128/361 (35.46%), Postives = 205/361 (56.79%), Query Frame = 3
Query: 618 FLVALKTGNLSEVLRLVD--SVGVNTNLKGMYPLLIAANEGHEDLVNLLLE---KKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDK--LEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHI 1673
F+ A G+L +VL L+ SV VN+ L A+ GH ++V L++ + +IN+ D DGD A+H A + + +L GAD+N++N + TP IAV KG ++L+ L+ +A + D D DT LH ++ + AL+ A +DI + N FN+ H AA+ G+ I L+ + +V+ +K+D + A+H+AA+NNH +L+K ADLN+QNI L+T LH+AV+R++ + VK LV++ A++ + DK+G+T LH A++ + T S + +L + G +K IA YL+ NG+ I IKN KG+TPLD+C D +L R L +SQ ++
Sbjct: 539 FVKAAANGDLDKVLELLKKRSVDVNSMFARHSALHAASQNGHVEIVQALIKYGGDQLDINLKDPDGDAAVHHAALGDHKIIIQILKNAGADINSRNRRNQTPLHIAVNKGHLEVLKALLELKSALQTNQDYDGDTPLHDSITRRHDKLTLALVQANSDITVCNNAGFNSLHHAALRGNPNATRIILENLPRQWIVDEKKDDGYTALHLAALNNHADVAELLIKVGSADLNVQNINLQTPLHLAVERQHSNMVKLLVDSGADISIPDKDGDTPLHEALR-HHTLSQLRQMQDLQDMGKLLMGLSLGGEKRSAAHIACYLVSNGSDIHIKNKKGQTPLDLCPDQNL-IRALKRCHENSQPPNV 897 HSP 2 Score: 53.1434 bits (126), Expect = 6.809e-7 Identity = 21/65 (32.31%), Postives = 37/65 (56.92%), Query Frame = 3
Query: 1713 DARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
D + + +L Q+ +++++ +C +C DR K++F CGH C C D + C +C E I +KI
Sbjct: 1226 DQSKKDKDYKKLEQQYQDIKEMTMCPVCMDRVRKMVFMCGHCSCQLCGDKLETCPICRETITNKI 1290
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Planmine SMEST
Match: SMESG000030607.1 (SMESG000030607.1) HSP 1 Score: 184.882 bits (468), Expect = 1.342e-48 Identity = 149/500 (29.80%), Postives = 239/500 (47.80%), Query Frame = 3
Query: 330 GSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKXXXXXXXXXXXXXXLITDERTMRF--------------LVALKTGNLSEVLRLV-------------------------------DSVGVNTNLKGMYPLLIAANEGHEDLVNLLLE---KKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKR-DGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKY-KADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTESGFGNFLKMTSALGVLGNIGEGLDK--LEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHI 1673
G +V++ D E K +Q G W E M +G +++Q +++ + + +S V N+ L + T RTM + A TG LS VLR + SV VN+ L A+ GH ++V L++ + +IN+ D DGD A+H A + + +L GAD+N++N + TP IAV KG ++L+ L+ S+ D D DT LH ++ + AL+ A +DI + N FN+ H AA+ G+ I L+ + +V+ +K+D + A+H+AA+NNH +L+K ADLN+QNI L+T LH+AV+R++ + VK LV++ A++ + DK+G+T LH A++ + T S + +L + G +K IA YL+ NG+ I IKN KG+TPLD+C D +L R L +SQ ++
Sbjct: 405 GCWVKISSDLELIKTVQCGLGMWNESMAVCVGTVGKIVQSLGHDLWRV-----VLSRTSGSSESTFVLNSALLARIRSPMSSPEVSVSATQPRTMLLSQRSTGSTSQPIEAVEAASTGTLSHVLRSIFEAHTTGDAKELFVKAAANGDLDKVLELLKKRSVDVNSMFARHSALHAASQNGHVEIVQALIKYGGDQLDINLKDPDGDAAVHHAALGDHKIIIQILKNAGADINSRNRRNQTPLHIAVNKGHLEVLKALLELK-CLASLQDYDGDTPLHDSITRRHDKLTLALVQANSDITVCNNAGFNSLHHAALRGNPNATRIILENLPRQWIVDEKKDDGYTALHLAALNNHADVAELLIKVGSADLNVQNINLQTPLHLAVERQHSNMVKLLVDSGADISIPDKDGDTPLHEALR-HHTLSQLRQMQDLQDMGKLLMGLSLGGEKRSAAHIACYLVSNGSDIHIKNKKGQTPLDLCPDQNL-IRALKRCHENSQPPNV 896 HSP 2 Score: 53.1434 bits (126), Expect = 7.414e-7 Identity = 21/65 (32.31%), Postives = 37/65 (56.92%), Query Frame = 3
Query: 1713 DARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKI 1907
D + + +L Q+ +++++ +C +C DR K++F CGH C C D + C +C E I +KI
Sbjct: 1225 DQSKKDKDYKKLEQQYQDIKEMTMCPVCMDRVRKMVFMCGHCSCQLCGDKLETCPICRETITNKI 1289 The following BLAST results are available for this feature:
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 4
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase mind-bomb vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035592 ID=SMED30035592|Name=E3 ubiquitin-protein ligase mind-bomb|organism=Schmidtea mediterranea sexual|type=transcript|length=2034bpback to top protein sequence of SMED30035592-orf-1 >SMED30035592-orf-1 ID=SMED30035592-orf-1|Name=SMED30035592-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=614bp MGANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLINback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
