Protein disulfide-isomerase A5
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035571 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 48Note: Hover over icons to view figure legend
Homology
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Human
Match: PDIA5 (protein disulfide isomerase family A member 5 [Source:HGNC Symbol;Acc:HGNC:24811]) HSP 1 Score: 167.933 bits (424), Expect = 9.497e-45 Identity = 109/338 (32.25%), Postives = 179/338 (52.96%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEG-DNSKEALIKFVHNPTQPPPQK-DVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM---AENKKVAFIGVDCT-DESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETH--LAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWC CK+M P F AAT L+G ++ G+N S +++K+ +++ G+P++ + G +F+Y+ ++ E +++++ NP P PQ + PW++E + LTD +F +++ +H SV+VMF+APWCGHCK KP+F AA + A++ V VD T +++ A+ I+ FP +YF KN + R + F+ +M NP P + +++ L +NF + + LVMFY+PWC HC + P F A+ + AAVD + + L Q V G+PT Y+ KY +RT+ T ++
Sbjct: 176 MFYAPWCSMCKRMMPHFQKAATQLRGHAVLAGMNVYSSEFENIKEEYSVRGFPTICYFEKGRFLFQYDNYGSTAEDIVEWLKNPQPPQPQVPETPWADEGGSVYHLTDEDFDQFVKEHSSVLVMFHAPWCGHCKKMKPEFEKAAEALHGEADSSGV-LAAVDATVNKALAE---RFHISEFPTLKYF---KNGEKYAVPVLRTKKKFLEWMQNPEAPPPPEPTWEEQ---QTSVLHLVGDNF-RETLKKKKHTLVMFYAPWCPHCKKVIPHFTATADAFKDDR-KIACAAVDCVKDKNQDLCQQEAVKGYPTFHYYHYGKFAEKYDSDRTELGFTNYI 501 HSP 2 Score: 126.331 bits (316), Expect = 3.240e-30 Identity = 95/322 (29.50%), Postives = 153/322 (47.52%), Query Frame = 1
Query: 49 FAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPS-----VFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHES-VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRH-WRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSC-LIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+ A +KG+ ++ + + L + + P +F QDG +Y + ++++ F+ +P PP ++ P +++ +D ++ +F L + E +++MFYAPWC CK P F AA Q+ + +A G++ F +I + GFP YF G+ + + +D V ++ NP PQ W G ++ L +F Q + H+S LVMF++PWCGHC MKP F AA L S ++AAVDAT LA ++ +S FPTL YF NG+ RT E+M
Sbjct: 67 LSTVAQAVKGQGTICWVDCGDAESRKLCKKMKVDLSPKDKKVELFHYQDGAFHTEYNRAVTFKSIVAFLKDPKGPPLWEEDPGAKDVVHLD--SEKDFRRLLKKEEKPLLIMFYAPWCSMCKRMMPHFQKAATQLRGHAVLA--GMNVYSSEFENIKEEYSVRGFPTICYFEKGRFLFQYD-NYGSTAEDIVEWLKNPQPPQPQVPETPWADEGG--SVYHLTDEDFDQFVKEHSS-VLVMFHAPWCGHCKKMKPEFEKAAEALHGEADSSGVLAAVDATVNKALAERFHISEFPTLKYFK-NGEKYAVPVLRTKKKFLEWM 379
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Human
Match: TXNDC5 (thioredoxin domain containing 5 [Source:HGNC Symbol;Acc:HGNC:21073]) HSP 1 Score: 154.451 bits (389), Expect = 1.478e-40 Identity = 96/358 (26.82%), Postives = 167/358 (46.65%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQF---AAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFV-----HNPTQPPPQKDVPWSEETNEIDF-LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSN-------------SEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
MF+APWCG+C++++P + +++ +++ D + D+ A + GYP++ L + G + KY+G + L ++ P P P+ + P + E + + L+ +NF ++ Q + I F+APWCGHCKA P + A + ++ V VDCT ++C+ + G+P +F GK E L+R E +T P L A + ++ L +NNF I + + FY+PWCGHC + P++ + K L IA VD T E ++ ++Y V G+PTL+ F ++ G R D++ F++ + D
Sbjct: 83 MFFAPWCGHCQRLQPTWNDLGDKYNSMEDAKVYVA-KVDCTAHSDVCSAQGVRGYPTLKLFKPGQEAVKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQGDHFI-KFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCTQHY--ELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYVESQLQRTETGATETVTPSEAPVLAAEPEADK----GTVLALTENNFDDTIAEGIT--FIKFYAPWCGHCKTLAPTWEELSKKEFPGLAGVKIAEVDCTAERNICSKYSVRGYPTLLLFRGGKKVSEHSGGRDLDSLHRFVLSQAKD 430 HSP 2 Score: 102.449 bits (254), Expect = 1.273e-22 Identity = 63/233 (27.04%), Postives = 111/233 (47.64%), Query Frame = 1
Query: 310 LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM--AENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPG----HEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
L A+ + + Q + VMF+APWCGHC+ +P + ++ E+ KV VDCT S D+C+++G+ G+P + F G+ +Y R+ +M N + P + + L +NF ++ + F++PWCGHC A+ P++ A L+ + I VD T L + +V G+PTL++F +Y G+R +++ E++
Sbjct: 65 LYTADMFTHGIQSAAHFVMFFAPWCGHCQRLQPTWNDLGDKYNSMEDAKVYVAKVDCTAHS--DVCSAQGVRGYPTLKLFKPGQEA--VKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQ--GDHFIKFFAPWCGHCKALAPTWEQLALGLEH-SETVKIGKVDCTQHYELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYV 290
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Human
Match: TXNDC5 (thioredoxin domain containing 5 [Source:HGNC Symbol;Acc:HGNC:21073]) HSP 1 Score: 134.42 bits (337), Expect = 2.242e-34 Identity = 87/320 (27.19%), Postives = 145/320 (45.31%), Query Frame = 1
Query: 106 DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFV-----HNPTQPPPQKDVPWSEETNEIDF-LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSN-------------SEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
D + D+ A + GYP++ L + G + KY+G + L ++ P P P+ + P + E + + L+ +NF ++ Q + I F+APWCGHCKA P + A + ++ V VDCT ++C+ + G+P +F GK E L+R E +T P L A + ++ L +NNF I + + FY+PWCGHC + P++ + K L IA VD T E ++ ++Y V G+PTL+ F ++ G R D++ F++ + D
Sbjct: 12 DCTAHSDVCSAQGVRGYPTLKLFKPGQEAVKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQGDHFI-KFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCTQHY--ELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYVESQLQRTETGATETVTPSEAPVLAAEPEADK----GTVLALTENNFDDTIAEGIT--FIKFYAPWCGHCKTLAPTWEELSKKEFPGLAGVKIAEVDCTAERNICSKYSVRGYPTLLLFRGGKKVSEHSGGRDLDSLHRFVLSQAKD 322 HSP 2 Score: 97.0561 bits (240), Expect = 2.223e-21 Identity = 67/228 (29.39%), Postives = 110/228 (48.25%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLK-GKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHN--------------PTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTS-AANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQL 639
F+APWCG+CK + P + A L+ +++ IG D + +L + GYP++ +DG KV +Y+G E+L ++V + P++ P P +++ + LT+ NF + + + + I FYAPWCGHCK P + + + V VDCT E +IC+ + G+P F GK SE+S R+ D F+ + A +L
Sbjct: 104 FFAPWCGHCKALAPTWEQLALGLEHSETVKIG-KVDCTQHYELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYVESQLQRTETGATETVTPSEAPVLAAEPEADKGT-VLALTENNFDDTIAEGITFI-KFYAPWCGHCKTLAPTWEELSKKEFPGLAGVKIAEVDCTAER--NICSKYSVRGYPTLLLFRGGKKV--SEHSGGRDLDSLHRFVLSQAKDEL 324
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Human
Match: PDIA4 (protein disulfide isomerase family A member 4 [Source:HGNC Symbol;Acc:HGNC:30167]) HSP 1 Score: 134.035 bits (336), Expect = 1.701e-32 Identity = 77/236 (32.63%), Postives = 120/236 (50.85%), Query Frame = 1
Query: 295 NEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAE-NKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKK 999
N + L DANF ++ ++V++ FYAPWCGHCK P++ AN + + + + +D T S + + ++G+P + G+ +Y R +++ VA + + P W P E ++L K NF +V+ LV FY+PWCGHC + P + AA +L P +A VDAT ET LA ++ VSG+PTL F G Y G R I ++MI++
Sbjct: 62 NGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASV--LASRFDVSGYPTIKILKKGQAV---DYEGSRTQEEIVAKVREVSQPD------W--TPPPEVTLVLTKENF-DEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFR-KGRPYDYNGPREKYGIVDYMIEQ 282 HSP 2 Score: 124.405 bits (311), Expect = 2.981e-29 Identity = 94/353 (26.63%), Postives = 157/353 (44.48%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAE-NKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKY----------MGERTDDAITEFMIKKHSDIIRHEE 1026
FYAPWCG+CKQ P++ A LK K I + D ++ L F+++GYP++ +++ G V YEG ++E ++ V +QP W+ LT NF E ++ + ++V FYAPWCGHCK P++ AA ++++ + + VD T E+ D+ ++G+P + F G+ +Y+ RE+ V +M + P P E + + + F+++ G V+ + G + +AAN L+ T T +A KVS LV P KY G D AI +F++K ++ H +
Sbjct: 86 FYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAV-DYEGSRTQEEIVAKVREVSQPD------WTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAET--DLAKRFDVSGYPTLKIFRKGRPY---DYNGPREKYGIVDYMIEQSGP-----------PSKEILTLKQVQEFLKD------GDDVIIIGVFKGESDPAYQQYQDAANNLREDY------KFHHTFSTEIAKFLKVSQG-QLVVMQPEKFQSKYEPRSHMMDVQQGSTQDSAIKDFVLKYALPLVGHRK 402 HSP 3 Score: 67.3958 bits (163), Expect = 4.289e-11 Identity = 49/168 (29.17%), Postives = 87/168 (51.79%), Query Frame = 1
Query: 556 NTSNSEYSLEREEDD------FV-AFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYM-GERTDDAITEFMIKKHSDIIRHEE 1026
+ S ++++E EE D FV AF P + ++ + G +V+ F ++ L+ FY+PWCGHC ++P + + A K + +IA +DAT + +YKV GFPT +YF+P+GD VK+ G+R + +++F+ + + + R +E
Sbjct: 481 DESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVV--GKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKG-QKGLVIAKMDATANDVPSDRYKVEGFPT-IYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKE 644 HSP 4 Score: 59.3066 bits (142), Expect = 1.440e-8 Identity = 28/83 (33.73%), Postives = 45/83 (54.22%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNK--VFKYE-GDNSKEALIKFVH 243
FYAPWCG+CKQ++P + + A KG+ + D + D + + G+P+++ G+K K+E GD E L KF+
Sbjct: 551 FYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIE 633
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Human
Match: PDIA4 (protein disulfide isomerase family A member 4 [Source:HGNC Symbol;Acc:HGNC:30167]) HSP 1 Score: 134.035 bits (336), Expect = 1.739e-32 Identity = 77/236 (32.63%), Postives = 120/236 (50.85%), Query Frame = 1
Query: 295 NEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAE-NKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKK 999
N + L DANF ++ ++V++ FYAPWCGHCK P++ AN + + + + +D T S + + ++G+P + G+ +Y R +++ VA + + P W P E ++L K NF +V+ LV FY+PWCGHC + P + AA +L P +A VDAT ET LA ++ VSG+PTL F G Y G R I ++MI++
Sbjct: 62 NGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASV--LASRFDVSGYPTIKILKKGQAV---DYEGSRTQEEIVAKVREVSQPD------W--TPPPEVTLVLTKENF-DEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFR-KGRPYDYNGPREKYGIVDYMIEQ 282 HSP 2 Score: 126.331 bits (316), Expect = 7.582e-30 Identity = 91/351 (25.93%), Postives = 157/351 (44.73%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAE-NKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVY--------FSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEE 1026
FYAPWCG+CKQ P++ A LK K I + D ++ L F+++GYP++ +++ G V YEG ++E ++ V +QP W+ LT NF E ++ + ++V FYAPWCGHCK P++ AA ++++ + + VD T E+ D+ ++G+P + F G+ +Y+ RE+ V +M + P P E + + + F+++ G V+ + G + +AAN L+ T T +A KVS +V + P + G D AI +F++K ++ H +
Sbjct: 86 FYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAV-DYEGSRTQEEIVAKVREVSQPD------WTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAET--DLAKRFDVSGYPTLKIFRKGRPY---DYNGPREKYGIVDYMIEQSGP-----------PSKEILTLKQVQEFLKD------GDDVIIIGVFKGESDPAYQQYQDAANNLREDY------KFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRK 401 HSP 3 Score: 67.3958 bits (163), Expect = 4.397e-11 Identity = 49/168 (29.17%), Postives = 87/168 (51.79%), Query Frame = 1
Query: 556 NTSNSEYSLEREEDD------FV-AFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYM-GERTDDAITEFMIKKHSDIIRHEE 1026
+ S ++++E EE D FV AF P + ++ + G +V+ F ++ L+ FY+PWCGHC ++P + + A K + +IA +DAT + +YKV GFPT +YF+P+GD VK+ G+R + +++F+ + + + R +E
Sbjct: 480 DESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVV--GKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKG-QKGLVIAKMDATANDVPSDRYKVEGFPT-IYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKE 643 HSP 4 Score: 59.3066 bits (142), Expect = 1.414e-8 Identity = 28/83 (33.73%), Postives = 45/83 (54.22%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNK--VFKYE-GDNSKEALIKFVH 243
FYAPWCG+CKQ++P + + A KG+ + D + D + + G+P+++ G+K K+E GD E L KF+
Sbjct: 550 FYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIE 632
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Celegans
Match: C14B9.2 (Probable protein disulfide-isomerase A4 [Source:UniProtKB/Swiss-Prot;Acc:P34329]) HSP 1 Score: 131.724 bits (330), Expect = 4.239e-32 Identity = 76/227 (33.48%), Postives = 116/227 (51.10%), Query Frame = 1
Query: 310 LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
LTD NF +L ++ SV+V FYAPWCGHCK P++ A+++++ + VD T E+ ++ I G+P +++ GK ++ Y R+E V ++ + +P P E +V L NF + +S+ LV FY+PWCGHC + P + AA KL+ + VDAT E L +Y VSG+PT+ NG Y G R I ++M
Sbjct: 41 LTDKNFDAFLKKNPSVLVKFYAPWCGHCKHLAPEYEKASSKVS----IPLAKVDATVET--ELGKRFEIQGYPTLKFWKDGKGPND--YDGGRDEAGIVEWVESRVDPNYK--------PPPEEVVTLTTENF-DDFISNNELVLVEFYAPWCGHCKKLAPEYEKAAQKLKAQGSKVKLGKVDATIEKDLGTKYGVSGYPTMKIIR-NGRRFDYNGPREAAGIIKYM 249 HSP 2 Score: 121.324 bits (303), Expect = 1.075e-28 Identity = 72/238 (30.25%), Postives = 122/238 (51.26%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPT----QPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM-AENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTN---PANPQLDARRHWRRIPGHEAIVIL 693
FYAPWCG+CK + P++ A++ + SI + D + +L + F I GYP++ +DG Y+G + ++++V + +PPP+ E+ LT NF +++ +E V+V FYAPWCGHCK P++ AA ++ A+ KV VD T E D+ T G++G+P + G+ +Y+ RE + +MT+ PA +L + R + + I+
Sbjct: 60 FYAPWCGHCKHLAPEYEKASSKV---SIPLA-KVDATVETELGKRFEIQGYPTLKFWKDGKGPNDYDGGRDEAGIVEWVESRVDPNYKPPPE----------EVVTLTTENFDDFISNNELVLVEFYAPWCGHCKKLAPEYEKAAQKLKAQGSKVKLGKVDATIEK--DLGTKYGVSGYPTMKIIRNGRRF---DYNGPREAAGIIKYMTDQSKPAAKKLPKLKDVERFMSKDDVTII 278 HSP 3 Score: 72.7886 bits (177), Expect = 3.425e-13 Identity = 42/142 (29.58%), Postives = 76/142 (53.52%), Query Frame = 1
Query: 592 EDDFVAFMTNPANPQLDARRHWRRIPGHE----AIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNG---DWVKYMGERTDDAITEFMIK 996
+++ AFM ++ + A+ H + P + + + +NF + + + L+ FY+PWCGHC + + +V A L+ P+ ++A +DAT +Q+ V GFPT +YF+P G + +KY G R + + +FM K
Sbjct: 469 DENLEAFMKQISSGK--AKAHVKSAPAPKDDKGPVKTVVGSNFDKIVNDESKDVLIEFYAPWCGHCKSFESKYVELAQALKKTQPNVVLAKMDATI-NDAPSQFAVEGFPT-IYFAPAGKKSEPIKYSGNRDLEDLKKFMTK 606 HSP 4 Score: 60.077 bits (144), Expect = 2.868e-9 Identity = 42/152 (27.63%), Postives = 70/152 (46.05%), Query Frame = 1
Query: 193 FKYEGDNSKEALIKFV-------HNPTQPPPQKDVPWSEETNEIDFLTDANFYEYL-DQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENK-KVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTN 621
F E D + EA +K + H + P P+ D + + +NF + + D+ + V++ FYAPWCGHCK+ + K+ A + + + V +D T D + + GFP + GK + +YS R+ +D FMT
Sbjct: 464 FDGELDENLEAFMKQISSGKAKAHVKSAPAPKDD------KGPVKTVVGSNFDKIVNDESKDVLIEFYAPWCGHCKSFESKYVELAQALKKTQPNVVLAKMDAT---INDAPSQFAVEGFPTIYFAPAGKKSEPIKYSGNRDLEDLKKFMTK 606 HSP 5 Score: 51.9878 bits (123), Expect = 9.611e-7 Identity = 26/81 (32.10%), Postives = 41/81 (50.62%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKV--FKYEGDNSKEALIKFV 240
FYAPWCG+CK + ++ A LK + + +T +D F + G+P+++ G K KY G+ E L KF+
Sbjct: 524 FYAPWCGHCKSFESKYVELAQALKKTQPNVVLAKMDATINDAPSQFAVEGFPTIYFAPAGKKSEPIKYSGNRDLEDLKKFM 604
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Celegans
Match: pdi-2 (Protein disulfide-isomerase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q17770]) HSP 1 Score: 108.227 bits (269), Expect = 2.484e-25 Identity = 100/343 (29.15%), Postives = 152/343 (44.31%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLK--GKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVA-FIGVDCTDE--SFADICTSEGITGF-PIFRYF---NYGKNTSNSEYSLEREEDDFVAFMTNPANPQL---DARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGD--WVKYMGERTDDAITEFM 990
FYAPWCG+CK + P++A AAT LK G I +G D + ++ F + GYP++ L ++G K +Y G +++I ++ T P + L DA+ + L + V+V+ G+ K T A + EN ++ F G+ DE + I E +T F P F N K T N ++ P L D W + P + IL NF Q +T LV FY+PWCGHC + P++ K S +IA +D+T + K+ FPT+ +F P G V Y G+RT + T+F+
Sbjct: 47 FYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLG-KLDATVHGEVSSKFEVRGYPTLKLFRNG-KPQEYNGGRDHDSIIAWLKKKTGPVAKP-------------LADADAVKELQESADVVVI------GYFKDTTS--DDAKTFLEENARIMEFFGLK-KDELPAIRLISLEEDMTKFKPDFEEITTENISKFTQN--------------YLDGSVKPHLMSEDIPEDWDKNP----VKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFAD-DESIVIAKMDST--LNEVEDVKIQSFPTIKFF-PAGSNKVVDYTGDRTIEGFTKFL 343 HSP 2 Score: 78.1814 bits (191), Expect = 2.454e-15 Identity = 38/118 (32.20%), Postives = 66/118 (55.93%), Query Frame = 1
Query: 664 IPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIR 1017
I E +++L K+NF +++ LV FY+PWCGHC ++ P + AA +L+ + +DAT ++++++V G+PTL F NG +Y G R D+I ++ KK + +
Sbjct: 19 IEEEENVIVLTKDNF-DEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFR-NGKPQEYNGGRDHDSIIAWLKKKTGPVAK 134 HSP 3 Score: 72.0182 bits (175), Expect = 2.849e-13 Identity = 38/117 (32.48%), Postives = 61/117 (52.14%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIG-VDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
EE + LT NF E ++ +E ++V FYAPWCGHCK+ P++ AA Q+ E +G +D T ++ + + G+P + F GK EY+ R+ D +A++ P
Sbjct: 20 EEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDAT--VHGEVSSKFEVRGYPTLKLFRNGK---PQEYNGGRDHDSIIAWLKKKTGP 131
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Celegans
Match: pdi-2 (Protein disulfide-isomerase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q17770]) HSP 1 Score: 108.227 bits (269), Expect = 2.484e-25 Identity = 100/343 (29.15%), Postives = 152/343 (44.31%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLK--GKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVA-FIGVDCTDE--SFADICTSEGITGF-PIFRYF---NYGKNTSNSEYSLEREEDDFVAFMTNPANPQL---DARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGD--WVKYMGERTDDAITEFM 990
FYAPWCG+CK + P++A AAT LK G I +G D + ++ F + GYP++ L ++G K +Y G +++I ++ T P + L DA+ + L + V+V+ G+ K T A + EN ++ F G+ DE + I E +T F P F N K T N ++ P L D W + P + IL NF Q +T LV FY+PWCGHC + P++ K S +IA +D+T + K+ FPT+ +F P G V Y G+RT + T+F+
Sbjct: 47 FYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLG-KLDATVHGEVSSKFEVRGYPTLKLFRNG-KPQEYNGGRDHDSIIAWLKKKTGPVAKP-------------LADADAVKELQESADVVVI------GYFKDTTS--DDAKTFLEENARIMEFFGLK-KDELPAIRLISLEEDMTKFKPDFEEITTENISKFTQN--------------YLDGSVKPHLMSEDIPEDWDKNP----VKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFAD-DESIVIAKMDST--LNEVEDVKIQSFPTIKFF-PAGSNKVVDYTGDRTIEGFTKFL 343 HSP 2 Score: 78.1814 bits (191), Expect = 2.454e-15 Identity = 38/118 (32.20%), Postives = 66/118 (55.93%), Query Frame = 1
Query: 664 IPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIR 1017
I E +++L K+NF +++ LV FY+PWCGHC ++ P + AA +L+ + +DAT ++++++V G+PTL F NG +Y G R D+I ++ KK + +
Sbjct: 19 IEEEENVIVLTKDNF-DEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFR-NGKPQEYNGGRDHDSIIAWLKKKTGPVAK 134 HSP 3 Score: 72.0182 bits (175), Expect = 2.849e-13 Identity = 38/117 (32.48%), Postives = 61/117 (52.14%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIG-VDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
EE + LT NF E ++ +E ++V FYAPWCGHCK+ P++ AA Q+ E +G +D T ++ + + G+P + F GK EY+ R+ D +A++ P
Sbjct: 20 EEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDAT--VHGEVSSKFEVRGYPTLKLFRNGK---PQEYNGGRDHDSIIAWLKKKTGP 131
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Celegans
Match: pdi-2 (Protein disulfide-isomerase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q17770]) HSP 1 Score: 108.227 bits (269), Expect = 6.164e-25 Identity = 100/375 (26.67%), Postives = 161/375 (42.93%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLK--GKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQP--PPQKDVPWSEETNE------IDFLTD---------------ANFYEYLDQHESVI---------VMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITG--FPIFRYFNYGKNTSNSEYSLEREEDDFVA-----FMTNPANPQL---DARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGD--WVKYMGERTDDAITEFM 990
FYAPWCG+CK + P++A AAT LK G I +G D + ++ F + GYP++ L ++G K +Y G +++I ++ T P P D +E E I + D A E+ + SVI ++F + + +F +AA Q KV F+ ++ E A I G+ P R + ++ + + E + ++ ++ P L D W + P + IL NF Q +T LV FY+PWCGHC + P++ K S +IA +D+T + K+ FPT+ +F P G V Y G+RT + T+F+
Sbjct: 47 FYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLG-KLDATVHGEVSSKFEVRGYPTLKLFRNG-KPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADVVVIGYFKDTTSDDAKTWIQANRLALVSEFTQETASVIFGGEIKSHNLLFVSKESSEFAKLEQEFKNAAKQF--KGKVLFVYINTDVEENARIMEFFGLKKDELPAIRLISLEEDMTKFKPDFEEITTENISKFTQNYLDGSVKPHLMSEDIPEDWDKNP----VKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFAD-DESIVIAKMDST--LNEVEDVKIQSFPTIKFF-PAGSNKVVDYTGDRTIEGFTKFL 409 HSP 2 Score: 78.1814 bits (191), Expect = 3.773e-15 Identity = 38/118 (32.20%), Postives = 66/118 (55.93%), Query Frame = 1
Query: 664 IPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIR 1017
I E +++L K+NF +++ LV FY+PWCGHC ++ P + AA +L+ + +DAT ++++++V G+PTL F NG +Y G R D+I ++ KK + +
Sbjct: 19 IEEEENVIVLTKDNF-DEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFR-NGKPQEYNGGRDHDSIIAWLKKKTGPVAK 134 HSP 3 Score: 72.0182 bits (175), Expect = 3.709e-13 Identity = 38/117 (32.48%), Postives = 61/117 (52.14%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIG-VDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
EE + LT NF E ++ +E ++V FYAPWCGHCK+ P++ AA Q+ E +G +D T ++ + + G+P + F GK EY+ R+ D +A++ P
Sbjct: 20 EEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDAT--VHGEVSSKFEVRGYPTLKLFRNGK---PQEYNGGRDHDSIIAWLKKKTGP 131
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Celegans
Match: pdi-2 (Protein disulfide-isomerase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q17770]) HSP 1 Score: 108.227 bits (269), Expect = 6.164e-25 Identity = 100/375 (26.67%), Postives = 161/375 (42.93%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLK--GKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQP--PPQKDVPWSEETNE------IDFLTD---------------ANFYEYLDQHESVI---------VMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITG--FPIFRYFNYGKNTSNSEYSLEREEDDFVA-----FMTNPANPQL---DARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGD--WVKYMGERTDDAITEFM 990
FYAPWCG+CK + P++A AAT LK G I +G D + ++ F + GYP++ L ++G K +Y G +++I ++ T P P D +E E I + D A E+ + SVI ++F + + +F +AA Q KV F+ ++ E A I G+ P R + ++ + + E + ++ ++ P L D W + P + IL NF Q +T LV FY+PWCGHC + P++ K S +IA +D+T + K+ FPT+ +F P G V Y G+RT + T+F+
Sbjct: 47 FYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLG-KLDATVHGEVSSKFEVRGYPTLKLFRNG-KPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADVVVIGYFKDTTSDDAKTWIQANRLALVSEFTQETASVIFGGEIKSHNLLFVSKESSEFAKLEQEFKNAAKQF--KGKVLFVYINTDVEENARIMEFFGLKKDELPAIRLISLEEDMTKFKPDFEEITTENISKFTQNYLDGSVKPHLMSEDIPEDWDKNP----VKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFAD-DESIVIAKMDST--LNEVEDVKIQSFPTIKFF-PAGSNKVVDYTGDRTIEGFTKFL 409 HSP 2 Score: 78.1814 bits (191), Expect = 3.773e-15 Identity = 38/118 (32.20%), Postives = 66/118 (55.93%), Query Frame = 1
Query: 664 IPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIR 1017
I E +++L K+NF +++ LV FY+PWCGHC ++ P + AA +L+ + +DAT ++++++V G+PTL F NG +Y G R D+I ++ KK + +
Sbjct: 19 IEEEENVIVLTKDNF-DEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFR-NGKPQEYNGGRDHDSIIAWLKKKTGPVAK 134 HSP 3 Score: 72.0182 bits (175), Expect = 3.709e-13 Identity = 38/117 (32.48%), Postives = 61/117 (52.14%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIG-VDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
EE + LT NF E ++ +E ++V FYAPWCGHCK+ P++ AA Q+ E +G +D T ++ + + G+P + F GK EY+ R+ D +A++ P
Sbjct: 20 EEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDAT--VHGEVSSKFEVRGYPTLKLFRNGK---PQEYNGGRDHDSIIAWLKKKTGP 131
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Fly
Match: CG9302 (gene:FBgn0032514 transcript:FBtr0080470) HSP 1 Score: 217.238 bits (552), Expect = 2.733e-63 Identity = 128/341 (37.54%), Postives = 180/341 (52.79%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTL--KGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNP-TQPPPQKDVP-WSEETN-EIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFI-GVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLE---REEDDFVAFMTNPANPQLDARRHWRRIPGHEA--IVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFY PWCG+CK+MKP++ A+T L KG I +N + +++ FNITG+P++ ++G F YEG+N+KEAL+ F+ NP +P P+ P WS +TN EI LT F L +S +VMFYAPWCGHCK KP++ AA +M + K + +D T E I + G+P ++F SN + E RE V FM +P P ++ ++ L+ +NF + + ALVMFY+PWCGHC KP F AA LQ P A+D T L A+Y V G+PT++YFS + Y G RT +M
Sbjct: 168 MFYVPWCGFCKKMKPEYGKASTELKTKGGYILAAMNVERQENAPIRKMFNITGFPTLIYFENGKLRFTYEGENNKEALVSFMLNPNAKPTPKPKEPEWSADTNSEIVHLTSQGFEPALKDEKSALVMFYAPWCGHCKRMKPEYEKAALEMKQKKIPGLLAALDATKEP--SIAEKYKVKGYPTVKFF------SNGVFKFEVNVREASKIVEFMRDPKEPPPPPPPEKSWEEEEDSKEVLFLDDDNF-SSTLKRKKHALVMFYAPWCGHCKHTKPEFTAAATALQD-DPRIAFVAIDCTKLAALCAKYNVRGYPTILYFSYLKTKLDYNGGRTSKDFIAYM 498 HSP 2 Score: 152.14 bits (383), Expect = 1.827e-39 Identity = 86/216 (39.81%), Postives = 131/216 (60.65%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDD--LKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEA--LIKFVHNPTQPPPQKDVPWSE----ETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNP 624
MFYAPWCG+CK+MKP++ AA +K K I G+ L T + + + + + GYP+V +G VFK+E N +EA +++F+ +P +PPP S ++ E+ FL D NF L + + +VMFYAPWCGHCK TKP+FT+AA + ++ ++AF+ +DCT A +C + G+P YF+Y K + +Y+ R DF+A+M NP
Sbjct: 294 MFYAPWCGHCKRMKPEYEKAALEMKQKKI-PGLLAALDATKEPSIAEKYKVKGYPTVKFFSNG--VFKFEV-NVREASKIVEFMRDPKEPPPPPPPEKSWEEEEDSKEVLFLDDDNFSSTLKRKKHALVMFYAPWCGHCKHTKPEFTAAATALQDDPRIAFVAIDCT--KLAALCAKYNVRGYPTILYFSYLK--TKLDYNGGRTSKDFIAYMNNP 501 HSP 3 Score: 144.436 bits (363), Expect = 8.255e-37 Identity = 100/320 (31.25%), Postives = 154/320 (48.12%), Query Frame = 1
Query: 49 FAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYP-SVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFL---TDANFYEYLDQH-ESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNP-ANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
F AA ++G + ++ L + ++ P ++ +DG+ Y+ S ++I F+ +P+ D+PW E+ D L A+F ++L + ++VMFY PWCG CK KP++ A+ ++ ++ + A I ITGFP YF GK Y E ++ V+FM NP A P + + IV L F + S ALVMFY+PWCGHC MKP + AA +++ L+AA+DAT E +A +YKV G+PT+ +FS NG + + R I EFM
Sbjct: 63 FREAAEAIRGTGTMLLLDCGQQDRKKLCKKLKVSPDPYAIKHYKDGDFHKDYDRQLSVSSMITFMRDPSG-----DLPWEEDPAGKDVLHFSDAASFTKHLRKDIRPMLVMFYVPWCGFCKKMKPEYGKASTELKTKGGYILAAMNVERQENAPIRKMFNITGFPTLIYFENGK--LRFTYEGENNKEALVSFMLNPNAKPTPKPKEPEWSADTNSEIVHLTSQGFEPALKDEKS-ALVMFYAPWCGHCKRMKPEYEKAALEMKQKKIPGLLAALDATKEPSIAEKYKVKGYPTVKFFS-NGVFKFEVNVREASKIVEFM 373 HSP 4 Score: 74.3294 bits (181), Expect = 1.031e-13 Identity = 45/178 (25.28%), Postives = 78/178 (43.82%), Query Frame = 1
Query: 466 VDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIP-GHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAA-VDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMI 993
+DC + +C ++ P + +Y + +Y + + FM +P+ W P G + + + +F +++ LVMFY PWCG C MKP + A+ +L+T L A V+ + + ++GFPTL+YF Y GE +A+ FM+
Sbjct: 79 LDCGQQDRKKLCKKLKVSPDP-YAIKHYKDGDFHKDYDRQLSVSSMITFMRDPS-----GDLPWEEDPAGKDVLHFSDAASFTKHLRKDIRPMLVMFYVPWCGFCKKMKPEYGKASTELKTKGGYILAAMNVERQENAPIRKMFNITGFPTLIYFENGKLRFTYEGENNKEALVSFML 250
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Fly
Match: prtp (gene:FBgn0030329 transcript:FBtr0303334) HSP 1 Score: 117.087 bits (292), Expect = 6.123e-28 Identity = 90/362 (24.86%), Postives = 149/362 (41.16%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKP---QFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNK-VFKYEGDNSKEALIKFVHNPTQPPPQKDVP--WSEETNEIDF-----LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILE------------------KNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATT--ETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIK 996
F+APWCG+CK+++P Q A K I + D + L +TGYP++ L + G + K++G A+ F++ P + D+ E+ ++ LT+ F +++ V F+APWC HC+ P + A ++ + V +DCT F IC + G+P + GK YS R+ ++ L+ G E +VI E ++ F Q I A + FY+PWCGHC ++P++ A + S IA VD T + +V G+PTL + +Y G R+ + ++ K
Sbjct: 60 FFAPWCGHCKRIQPLWEQLAEIMNVDNPKVIIAKV--DCTKHQGLCATHQVTGYPTLRLFKLGEEESVKFKGTRDLPAITDFINKELSAPAEADLGEVKREQVENLNIGKVVDLTEDTFAKHVSTGNH-FVKFFAPWCSHCQRLAPTWEDLAKELIKEPTVTISKIDCT--QFRSICQDFEVKGYPTLLWIEDGKKIEK--YSGARDLSTLKTYVEKMVGVPLEKTAG---EAGDEKVVIEEVAGEEDAAKKLTPQQLTGEDEFDQAIAEGV--AFIKFYAPWCGHCQKLQPTWEQLATETHQAQSSVKIAKVDCTAPENKQVCIDQQVEGYPTLFLYKNGQRQNEYEGSRSLPELQAYLKK 409 HSP 2 Score: 102.064 bits (253), Expect = 6.191e-23 Identity = 62/210 (29.52%), Postives = 101/210 (48.10%), Query Frame = 1
Query: 352 SVIVMFYAPWCGHCKATKPKFTSAANQM-AENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAF----MTNPANPQL-DARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGER 963
+V V F+APWCGHCK +P + A M +N KV VDCT +C + +TG+P R F G+ S ++ R+ F ++ PA L + +R +V L ++ F +++ T V F++PWC HC + P++ + A +L P+ I+ +D T + ++V G+PTL++ KY G R
Sbjct: 55 NVFVKFFAPWCGHCKRIQPLWEQLAEIMNVDNPKVIIAKVDCTKHQ--GLCATHQVTGYPTLRLFKLGEEES-VKFKGTRDLPAITDFINKELSAPAEADLGEVKREQVENLNIGKVVDLTEDTFAKHV--STGNHFVKFFAPWCSHCQRLAPTWEDLAKEL-IKEPTVTISKIDCTQFRSICQDFEVKGYPTLLWIEDGKKIEKYSGAR 258 HSP 3 Score: 65.0846 bits (157), Expect = 7.244e-11 Identity = 34/89 (38.20%), Postives = 50/89 (56.18%), Query Frame = 1
Query: 742 LVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFS-PNGDWVKYMGERTDDAITEFMIKKHS 1005
V F++PWCGHC ++P + A + P +IA VD T L A ++V+G+PTL F + VK+ G R AIT+F+ K+ S
Sbjct: 57 FVKFFAPWCGHCKRIQPLWEQLAEIMNVDNPKVIIAKVDCTKHQGLCATHQVTGYPTLRLFKLGEEESVKFKGTRDLPAITDFINKELS 145
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Fly
Match: prtp (gene:FBgn0030329 transcript:FBtr0303335) HSP 1 Score: 117.087 bits (292), Expect = 6.123e-28 Identity = 90/362 (24.86%), Postives = 149/362 (41.16%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKP---QFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNK-VFKYEGDNSKEALIKFVHNPTQPPPQKDVP--WSEETNEIDF-----LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILE------------------KNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATT--ETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIK 996
F+APWCG+CK+++P Q A K I + D + L +TGYP++ L + G + K++G A+ F++ P + D+ E+ ++ LT+ F +++ V F+APWC HC+ P + A ++ + V +DCT F IC + G+P + GK YS R+ ++ L+ G E +VI E ++ F Q I A + FY+PWCGHC ++P++ A + S IA VD T + +V G+PTL + +Y G R+ + ++ K
Sbjct: 60 FFAPWCGHCKRIQPLWEQLAEIMNVDNPKVIIAKV--DCTKHQGLCATHQVTGYPTLRLFKLGEEESVKFKGTRDLPAITDFINKELSAPAEADLGEVKREQVENLNIGKVVDLTEDTFAKHVSTGNH-FVKFFAPWCSHCQRLAPTWEDLAKELIKEPTVTISKIDCT--QFRSICQDFEVKGYPTLLWIEDGKKIEK--YSGARDLSTLKTYVEKMVGVPLEKTAG---EAGDEKVVIEEVAGEEDAAKKLTPQQLTGEDEFDQAIAEGV--AFIKFYAPWCGHCQKLQPTWEQLATETHQAQSSVKIAKVDCTAPENKQVCIDQQVEGYPTLFLYKNGQRQNEYEGSRSLPELQAYLKK 409 HSP 2 Score: 102.064 bits (253), Expect = 6.191e-23 Identity = 62/210 (29.52%), Postives = 101/210 (48.10%), Query Frame = 1
Query: 352 SVIVMFYAPWCGHCKATKPKFTSAANQM-AENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAF----MTNPANPQL-DARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGER 963
+V V F+APWCGHCK +P + A M +N KV VDCT +C + +TG+P R F G+ S ++ R+ F ++ PA L + +R +V L ++ F +++ T V F++PWC HC + P++ + A +L P+ I+ +D T + ++V G+PTL++ KY G R
Sbjct: 55 NVFVKFFAPWCGHCKRIQPLWEQLAEIMNVDNPKVIIAKVDCTKHQ--GLCATHQVTGYPTLRLFKLGEEES-VKFKGTRDLPAITDFINKELSAPAEADLGEVKREQVENLNIGKVVDLTEDTFAKHV--STGNHFVKFFAPWCSHCQRLAPTWEDLAKEL-IKEPTVTISKIDCTQFRSICQDFEVKGYPTLLWIEDGKKIEKYSGAR 258 HSP 3 Score: 65.0846 bits (157), Expect = 7.244e-11 Identity = 34/89 (38.20%), Postives = 50/89 (56.18%), Query Frame = 1
Query: 742 LVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFS-PNGDWVKYMGERTDDAITEFMIKKHS 1005
V F++PWCGHC ++P + A + P +IA VD T L A ++V+G+PTL F + VK+ G R AIT+F+ K+ S
Sbjct: 57 FVKFFAPWCGHCKRIQPLWEQLAEIMNVDNPKVIIAKVDCTKHQGLCATHQVTGYPTLRLFKLGEEESVKFKGTRDLPAITDFINKELS 145
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Fly
Match: prtp (gene:FBgn0030329 transcript:FBtr0073551) HSP 1 Score: 117.087 bits (292), Expect = 6.123e-28 Identity = 90/362 (24.86%), Postives = 149/362 (41.16%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKP---QFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNK-VFKYEGDNSKEALIKFVHNPTQPPPQKDVP--WSEETNEIDF-----LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILE------------------KNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATT--ETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIK 996
F+APWCG+CK+++P Q A K I + D + L +TGYP++ L + G + K++G A+ F++ P + D+ E+ ++ LT+ F +++ V F+APWC HC+ P + A ++ + V +DCT F IC + G+P + GK YS R+ ++ L+ G E +VI E ++ F Q I A + FY+PWCGHC ++P++ A + S IA VD T + +V G+PTL + +Y G R+ + ++ K
Sbjct: 60 FFAPWCGHCKRIQPLWEQLAEIMNVDNPKVIIAKV--DCTKHQGLCATHQVTGYPTLRLFKLGEEESVKFKGTRDLPAITDFINKELSAPAEADLGEVKREQVENLNIGKVVDLTEDTFAKHVSTGNH-FVKFFAPWCSHCQRLAPTWEDLAKELIKEPTVTISKIDCT--QFRSICQDFEVKGYPTLLWIEDGKKIEK--YSGARDLSTLKTYVEKMVGVPLEKTAG---EAGDEKVVIEEVAGEEDAAKKLTPQQLTGEDEFDQAIAEGV--AFIKFYAPWCGHCQKLQPTWEQLATETHQAQSSVKIAKVDCTAPENKQVCIDQQVEGYPTLFLYKNGQRQNEYEGSRSLPELQAYLKK 409 HSP 2 Score: 102.064 bits (253), Expect = 6.191e-23 Identity = 62/210 (29.52%), Postives = 101/210 (48.10%), Query Frame = 1
Query: 352 SVIVMFYAPWCGHCKATKPKFTSAANQM-AENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAF----MTNPANPQL-DARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGER 963
+V V F+APWCGHCK +P + A M +N KV VDCT +C + +TG+P R F G+ S ++ R+ F ++ PA L + +R +V L ++ F +++ T V F++PWC HC + P++ + A +L P+ I+ +D T + ++V G+PTL++ KY G R
Sbjct: 55 NVFVKFFAPWCGHCKRIQPLWEQLAEIMNVDNPKVIIAKVDCTKHQ--GLCATHQVTGYPTLRLFKLGEEES-VKFKGTRDLPAITDFINKELSAPAEADLGEVKREQVENLNIGKVVDLTEDTFAKHV--STGNHFVKFFAPWCSHCQRLAPTWEDLAKEL-IKEPTVTISKIDCTQFRSICQDFEVKGYPTLLWIEDGKKIEKYSGAR 258 HSP 3 Score: 65.0846 bits (157), Expect = 7.244e-11 Identity = 34/89 (38.20%), Postives = 50/89 (56.18%), Query Frame = 1
Query: 742 LVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFS-PNGDWVKYMGERTDDAITEFMIKKHS 1005
V F++PWCGHC ++P + A + P +IA VD T L A ++V+G+PTL F + VK+ G R AIT+F+ K+ S
Sbjct: 57 FVKFFAPWCGHCKRIQPLWEQLAEIMNVDNPKVIIAKVDCTKHQGLCATHQVTGYPTLRLFKLGEEESVKFKGTRDLPAITDFINKELS 145
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Fly
Match: prtp (gene:FBgn0030329 transcript:FBtr0332954) HSP 1 Score: 105.145 bits (261), Expect = 2.521e-24 Identity = 86/357 (24.09%), Postives = 144/357 (40.34%), Query Frame = 1
Query: 19 CGYCKQMKP---QFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNK-VFKYEGDNSKEALIKFVHNPTQPPPQKDVP--WSEETNEIDF-----LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILE------------------KNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATT--ETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIK 996
CG+CK+++P Q A K I + D + L +TGYP++ L + G + K++G A+ F++ P + D+ E+ ++ LT+ F +++ V F+APWC HC+ P + A ++ + V +DCT F IC + G+P + GK YS R+ ++ L+ G E +VI E ++ F Q I A + FY+PWCGHC ++P++ A + S IA VD T + +V G+PTL + +Y G R+ + ++ K
Sbjct: 2 CGHCKRIQPLWEQLAEIMNVDNPKVIIAKV--DCTKHQGLCATHQVTGYPTLRLFKLGEEESVKFKGTRDLPAITDFINKELSAPAEADLGEVKREQVENLNIGKVVDLTEDTFAKHVSTGNH-FVKFFAPWCSHCQRLAPTWEDLAKELIKEPTVTISKIDCT--QFRSICQDFEVKGYPTLLWIEDGKKIEK--YSGARDLSTLKTYVEKMVGVPLEKTAG---EAGDEKVVIEEVAGEEDAAKKLTPQQLTGEDEFDQAIAEGV--AFIKFYAPWCGHCQKLQPTWEQLATETHQAQSSVKIAKVDCTAPENKQVCIDQQVEGYPTLFLYKNGQRQNEYEGSRSLPELQAYLKK 346 HSP 2 Score: 90.5077 bits (223), Expect = 2.529e-19 Identity = 57/221 (25.79%), Postives = 91/221 (41.18%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQK--------DVPWSEETNEIDF--------LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENK-KVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFM 615
F+APWC +C+++ P + A L + D + + Q F + GYP++ I+DG K+ KY G L +V P +K V E E D LT + ++ + FYAPWCGHC+ +P + A + + + V VDCT +C + + G+P F Y +EY R + A++
Sbjct: 126 FFAPWCSHCQRLAPTWEDLAKELIKEPTVTISKIDCTQFRSICQDFEVKGYPTLLWIEDGKKIEKYSGARDLSTLKTYVEKMVGVPLEKTAGEAGDEKVVIEEVAGEEDAAKKLTPQQLTGEDEFDQAIAEGVAFIKFYAPWCGHCQKLQPTWEQLATETHQAQSSVKIAKVDCTAPENKQVCIDQQVEGYPTL--FLYKNGQRQNEYEGSRSLPELQAYL 344 HSP 3 Score: 52.7582 bits (125), Expect = 4.977e-7 Identity = 30/81 (37.04%), Postives = 44/81 (54.32%), Query Frame = 1
Query: 766 CGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFS-PNGDWVKYMGERTDDAITEFMIKKHS 1005
CGHC ++P + A + P +IA VD T L A ++V+G+PTL F + VK+ G R AIT+F+ K+ S
Sbjct: 2 CGHCKRIQPLWEQLAEIMNVDNPKVIIAKVDCTKHQGLCATHQVTGYPTLRLFKLGEEESVKFKGTRDLPAITDFINKELS 82
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Zebrafish
Match: pdia5 (protein disulfide isomerase family A, member 5 [Source:ZFIN;Acc:ZDB-GENE-030521-5]) HSP 1 Score: 201.445 bits (511), Expect = 3.728e-57 Identity = 125/339 (36.87%), Postives = 179/339 (52.80%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYE--GDNSKEALIKFVHNPTQPPPQK-DVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSE--GITGFPIFRYFNYGKNTSNSEYSLE--REEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATT-ETH-LAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWCG CK+M+P F AAT KGK + G+N + D +KQ F++ GYP+ + G + YE G SK+ + ++ NP P P+ +VPWSE + + LTD +F +L++H S ++MFYAPWCGHCK KP++ AA + NK GV ++ T E I+GFP +YF G+ +Y+L R +D + ++ NP P + W +P ++ L +F +++ ALVMFY+PWC HC P F AA + AAVD T + H L Q V G+PT Y++ KY GER + T FM
Sbjct: 184 MFYAPWCGVCKRMQPIFQQAATETKGKYVLAGMNVHPAEFDGVKQEFSVKGYPTFCYFEKGKFLHHYENYGATSKD-ITDWLKNPQPPQPKTPEVPWSESGSAVFHLTDDSFDSFLEEHPSALIMFYAPWCGHCKKMKPEYDDAAETL--NKDPNSPGVLAAVDTTIHKSTGERFKISGFPTVKYFEKGEE----KYTLPHLRSKDKIIEWLKNPQAPP-PPEKSWDEMPS--SVSHLGAEDFRESL-KKKKHALVMFYAPWCPHCKNAVPHFTTAAEMFKEDR-KIAYAAVDCTKGQNHELCKQEGVEGYPTFNYYNYGKFSEKYNGERGEAGFTGFM 510 HSP 2 Score: 169.088 bits (427), Expect = 2.642e-45 Identity = 82/209 (39.23%), Postives = 120/209 (57.42%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTT--DDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTN 621
MFYAPWCG+CK+MKP++ AA TL G+ + TT + F I+G+P+V + G + + SK+ +I+++ NP PPP + W E + + L +F E L + + +VMFYAPWC HCK P FT+AA E++K+A+ VDCT ++C EG+ G+P F Y+NYGK + +Y+ ER E F FM N
Sbjct: 307 MFYAPWCGHCKKMKPEYDDAAETLNKDPNSPGVLAAVDTTIHKSTGERFKISGFPTVKYFEKGEEKYTLPHLRSKDKIIEWLKNPQAPPPPEKS-WDEMPSSVSHLGAEDFRESLKKKKHALVMFYAPWCPHCKNAVPHFTTAAEMFKEDRKIAYAAVDCTKGQNHELCKQEGVEGYPTFNYYNYGKFS--EKYNGERGEAGFTGFMRN 512 HSP 3 Score: 124.79 bits (312), Expect = 7.518e-30 Identity = 83/277 (29.96%), Postives = 136/277 (49.10%), Query Frame = 1
Query: 175 QDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHES-VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHE---AIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPS-CLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+DG +Y + ++++ F+ +P P ++ P +++ I+ ++ +F + L + E +++MFYAPWCG CK +P F AA + K G++ F + + G+P F YF GK + E + D ++ NP PQ +P E A+ L ++F + H S AL+MFY+PWCGHC MKP + +AA L S ++AAVD T ++K+SGFPT+ YF + R+ D I E++
Sbjct: 122 KDGTFHTEYNRPATIKSMVAFLKDPAGAPLWEENPEAKDVVHIE--SEKDFRKLLKREERPILMMFYAPWCGVCKRMQPIFQQAATET--KGKYVLAGMNVHPAEFDGVKQEFSVKGYPTFCYFEKGKFLHHYE-NYGATSKDITDWLKNPQPPQPKT----PEVPWSESGSAVFHLTDDSFDSFLEEHPS-ALIMFYAPWCGHCKKMKPEYDDAAETLNKDPNSPGVLAAVDTTIHKSTGERFKISGFPTVKYFEKGEEKYTLPHLRSKDKIIEWL 388 HSP 4 Score: 57.3806 bits (137), Expect = 2.960e-8 Identity = 42/162 (25.93%), Postives = 71/162 (43.83%), Query Frame = 1
Query: 466 VDCTDESFADICT------SEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILE-KNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTET--HLAAQYKVSGFPTLVYF 924
V+C D +C S G + Y + T ++EY+ VAF+ +PA L W P + +V +E + +F + + L+MFY+PWCG C M+P F AA + + ++A ++ + ++ V G+PT YF
Sbjct: 91 VNCGDSEGRKLCKKVKVDPSSKTKGVELLHYKD---GTFHTEYNRPATIKSMVAFLKDPAGAPL-----WEENPEAKDVVHIESEKDFRKLLKREERPILMMFYAPWCGVCKRMQPIFQQAATETKG---KYVLAGMNVHPAEFDGVKQEFSVKGYPTFCYF 241
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Zebrafish
Match: pdia4 (protein disulfide isomerase family A, member 4 [Source:ZFIN;Acc:ZDB-GENE-030131-5493]) HSP 1 Score: 140.584 bits (353), Expect = 6.929e-35 Identity = 86/261 (32.95%), Postives = 131/261 (50.19%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKK-VAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKKKAD 1065
+E N + LTDANF +++ ++V+V FYAPWCGHCK P++ A + EN + VD T S + + ++G+P + G+ +Y +R E V + A P W+ P EA ++L K+NF ++V++ LV FY+PWCGHC + P + AA +L P +A VDAT E+ LA ++ VSG+PTL F G Y G R I ++M + + + K QE + D
Sbjct: 56 KEENGVLVLTDANFDTFIEGKDTVLVEFYAPWCGHCKQFAPEYEKIAQTLKENDPPIPVAKVDATKAS--GLGSRFEVSGYPTIKILKKGEPL---DYDGDRSEHAIVERVKEVAQPD------WK--PPPEATLVLTKDNF-DDVVNNADIILVEFYAPWCGHCKRLAPEYEKAAKELSNRTPPIPLAKVDATAESDLATRFGVSGYPTLKIFR-KGKAFDYNGPREKFGIVDYMSDQAGPPSKQVQTLKQVQELLRDGD 301 HSP 2 Score: 126.331 bits (316), Expect = 3.755e-30 Identity = 70/212 (33.02%), Postives = 111/212 (52.36%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMA-ENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
FYAPWCG+CKQ P++ A TLK I + D + L F ++GYP++ +++ G + Y+GD S+ A+++ V QP W LT NF + ++ + ++V FYAPWCGHCK P++ AA +++ + VD T ES D+ T G++G+P + F GK +Y+ RE+ V +M++ A P
Sbjct: 83 FYAPWCGHCKQFAPEYEKIAQTLKENDPPIPVAKVDATKASGLGSRFEVSGYPTIKILKKGEPL-DYDGDRSEHAIVERVKEVAQPD------WKPPPEATLVLTKDNFDDVVNNADIILVEFYAPWCGHCKRLAPEYEKAAKELSNRTPPIPLAKVDATAES--DLATRFGVSGYPTLKIFRKGKAF---DYNGPREKFGIVDYMSDQAGP 282 HSP 3 Score: 68.1662 bits (165), Expect = 1.807e-11 Identity = 46/169 (27.22%), Postives = 84/169 (49.70%), Query Frame = 1
Query: 562 SNSEYSLEREEDD-------FVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYMGERTDDAITEF--MIKKHSDIIRHEEAE 1032
+Y++E EE D +AF P + ++ + G +V+ F + ++ L+ FY+PWCGHC ++P +++ K + + +IA +DAT YKV GFPT +YF+P+ + +K+ G + D + EF ++KH+ + H++ E
Sbjct: 479 GGKKYAMEPEEFDSDVLRSFVMAFKKGKLKPIVKSQPLPKNNKGPVKVVV--GKTFDEIVMDSKKDVLIEFYAPWCGHCKKLEPDYISLGKKYKN-EKNLVIAKMDATANDVPHDSYKVEGFPT-IYFAPSNNKQNPIKFEGGKRD--VEEFSKFVEKHATKLSHKKDE 641 HSP 4 Score: 64.6994 bits (156), Expect = 1.797e-10 Identity = 29/90 (32.22%), Postives = 51/90 (56.67%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQK 270
FYAPWCG+CK++ P++ AA L ++ I + D + DL F ++GYP++ + + G K F Y G K ++ ++ + PP ++
Sbjct: 198 FYAPWCGHCKRLAPEYEKAAKELSNRTPPIPLAKVDATAESDLATRFGVSGYPTLKIFRKG-KAFDYNGPREKFGIVDYMSDQAGPPSKQ 286 HSP 5 Score: 50.8322 bits (120), Expect = 3.736e-6 Identity = 24/84 (28.57%), Postives = 41/84 (48.81%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKV--FKYEGDNSK-EALIKFVHN 246
FYAPWCG+CK+++P + + K + + D + D ++ + G+P+++ NK K+EG E KFV
Sbjct: 547 FYAPWCGHCKKLEPDYISLGKKYKNEKNLVIAKMDATANDVPHDSYKVEGFPTIYFAPSNNKQNPIKFEGGKRDVEEFSKFVEK 630 HSP 6 Score: 50.447 bits (119), Expect = 6.053e-6 Identity = 25/77 (32.47%), Postives = 35/77 (45.45%), Query Frame = 1
Query: 337 LDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSN 567
+D + V++ FYAPWCGHCK +P + S + K + +D T S + GFP YF N N
Sbjct: 537 MDSKKDVLIEFYAPWCGHCKKLEPDYISLGKKYKNEKNLVIAKMDATANDVPH--DSYKVEGFPTI-YFAPSNNKQN 610
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Zebrafish
Match: txndc5 (thioredoxin domain containing 5 [Source:ZFIN;Acc:ZDB-GENE-040426-1951]) HSP 1 Score: 132.494 bits (332), Expect = 3.480e-33 Identity = 93/359 (25.91%), Postives = 158/359 (44.01%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQA---FNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFV------HNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPAN-------PQLDARRHWRRIP-------GHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
MF+APWCG+C++++ + A + T D K I GYP++ L + + KY+G +AL ++ + E + LT NF ++ + S V F+APWCGHCKA P + A+ + + VDCT ++C+ + G+P +F G+ +Y +R+ D F F+ N P+ + H IP +++L ++NF + + S + FY+PWCGHC + P++ + + K L IA VD T E L ++ V G+PTL+ F ++ G R +++ F++K+ D
Sbjct: 51 MFFAPWCGHCQRLQGTWNELADKYNSMEAPPAYVVKVDCTKDTKFCSIEHGIRGYPTLKLFKPEQEAVKYQGPRDLQALENWMLKTLQEEPEEPQSEPEPPKVPEPKQGLYELTATNFKSHIAK-GSHFVKFFAPWCGHCKAMAPTWEQLASSFEHSDSIKISKVDCTQH--YEVCSDNQVRGYPTLLFFTDGEKID--QYKGKRDLDSFKEFVDNHVKAAESKDEPEKE-EEHTHEIPPSAEPEKQESNVLVLTESNFDETVAKGLS--FIKFYAPWCGHCKNLAPTWDDLSQKEFPGLTDVKIAKVDCTVERTLCNRFSVRGYPTLLMFRAGQQGEEHNGGRDLESLHSFIMKQARD 401 HSP 2 Score: 88.9669 bits (219), Expect = 1.706e-18 Identity = 59/216 (27.31%), Postives = 96/216 (44.44%), Query Frame = 1
Query: 358 IVMFYAPWCGHCKATKPKFTSAANQM--AENKKVAFIGVDCT-DESFADICTSEGITGFPIFRYFNYGKNTSNSE--YSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
VMF+APWCGHC+ + + A++ E + VDCT D F I GI G+P + F + + L+ E+ + + + + + L NF +I V F++PWCGHC AM P++ A+ + S I+ VD T + + +V G+PTL++F+ +Y G+R D+ EF+
Sbjct: 49 FVMFFAPWCGHCQRLQGTWNELADKYNSMEAPPAYVVKVDCTKDTKFCSI--EHGIRGYPTLKLFKPEQEAVKYQGPRDLQALENWMLKTLQEEPEEPQSEPEPPKVPEPKQGLYELTATNFKSHIAK--GSHFVKFFAPWCGHCKAMAPTWEQLASSFEHS-DSIKISKVDCTQHYEVCSDNQVRGYPTLLFFTDGEKIDQYKGKRDLDSFKEFV 259 HSP 3 Score: 66.6254 bits (161), Expect = 2.643e-11 Identity = 31/91 (34.07%), Postives = 52/91 (57.14%), Query Frame = 1
Query: 730 TSGALVMFYSPWCGHCIAMKPSFVNAANKLQTL-LPSCLIAAVDATTETHLAA-QYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIK 996
T+ VMF++PWCGHC ++ ++ A+K ++ P + VD T +T + ++ + G+PTL F P + VKY G R A+ +M+K
Sbjct: 45 TAPHFVMFFAPWCGHCQRLQGTWNELADKYNSMEAPPAYVVKVDCTKDTKFCSIEHGIRGYPTLKLFKPEQEAVKYQGPRDLQALENWMLK 135
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Zebrafish
Match: pdia6 (protein disulfide isomerase family A, member 6 [Source:ZFIN;Acc:ZDB-GENE-030131-879]) HSP 1 Score: 109.383 bits (272), Expect = 3.907e-25 Identity = 75/266 (28.20%), Postives = 122/266 (45.86%), Query Frame = 1
Query: 292 TNEIDFLTDANFYEYLDQHESV-IVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDAR-------RHWRRIPGHEA-----IVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCL-IAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQE 1047
++++ L +NF + Q +S+ +V FYAPWCGHCK+ P++ AA + KV + D + G+ GFP + F G +Y R V N + R + R G A +V L +NF + ++ LV F++PWCGHC ++P + AA +++ + +AAVDAT LA+++ + GFPT+ F + Y G RT I ++ +SD I E +++ E
Sbjct: 24 SDDVVELNPSNFNREVIQSDSLWLVEFYAPWCGHCKSLAPEWKKAATALKGIVKVGAVDAD----QHNSLGGQYGVRGFPTIKIFG-GNKHKPEDYQGGRTNQAIVDAALNALRSLVKDRLGGKTGGSDYSRQSGGGAGNKKDVVELTDDNFDRTVLESDDVWLVEFFAPWCGHCKNLEPEWTAAATEVKEQTKGKVKLAAVDATVHQGLASRFGIRGFPTIKVFRKGEEPEDYQGGRTRSDIVARALELYSDNIPAPELQEVLNE 284 HSP 2 Score: 98.2117 bits (243), Expect = 2.159e-21 Identity = 67/223 (30.04%), Postives = 106/223 (47.53%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNK--VFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDF----------------LTDANFYEYLDQHESV-IVMFYAPWCGHCKATKPKFTSAANQMAEN--KKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVA 609
FYAPWCG+CK + P++ AAT LKG ++ D + L + + G+P++ + GNK Y+G + +A++ N + + + +T D+ LTD NF + + + V +V F+APWCGHCK +P++T+AA ++ E KV VD T + + GI GFP + F G+ + Y R D VA
Sbjct: 50 FYAPWCGHCKSLAPEWKKAATALKGIVKVGAVDAD--QHNSLGGQYGVRGFPTIKIF-GGNKHKPEDYQGGRTNQAIVDAALNALRSLVKDRL--GGKTGGSDYSRQSGGGAGNKKDVVELTDDNFDRTVLESDDVWLVEFFAPWCGHCKNLEPEWTAAATEVKEQTKGKVKLAAVDAT--VHQGLASRFGIRGFPTIKVFRKGEEPED--YQGGRTRSDIVA 263
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Zebrafish
Match: dnajc10 (DnaJ (Hsp40) homolog, subfamily C, member 10 [Source:ZFIN;Acc:ZDB-GENE-070327-1]) HSP 1 Score: 99.7525 bits (247), Expect = 2.068e-21 Identity = 80/357 (22.41%), Postives = 145/357 (40.62%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQH---ESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKKKAD 1065
F+APWC C+ + P+ A+ L G+ F + D + + L +NI YP+ +I + + + +YEG +S + +++F+ + P + L +F E + + E+ +V FYAPWCG C+A P++ A ++ V VDC + C SE + +P R F N + + D + + W A V L +F + ++ ++ FY+PWCG C P F A ++ + + VD + +PT+ ++ G + G ++ +I +DI+R + Q + K D
Sbjct: 471 FFAPWCPPCRALLPELRKASIQLFGQLKFGTL--DCTIHEGLCNTYNIHAYPTT-VIFNKSSIHEYEGHHSADGILEFIEDLVNPV-------------VVTLGPESFQELVKRRKSSETWMVDFYAPWCGPCQALLPEWRRMARMLSGIVNVG--TVDC--QKHHSFCQSESVRAYPEIRLFPQNSNRRDQYQTYNGWHRDAFSL------------KAWALSSLPRASVDLSPEDFKRKVLGGKDHWVLDFYAPWCGPCQQFAPEFEVLARMMKGTVRA---GKVDCQAHYQTCQSAGIKAYPTVRFYPTLGTTRRDQGGEHINSRDATVI---ADILRQRLQQLALQGKSKLKD 789 HSP 2 Score: 68.1662 bits (165), Expect = 1.650e-11 Identity = 50/201 (24.88%), Postives = 81/201 (40.30%), Query Frame = 1
Query: 343 QHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGA--LVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGD 939
+ E +V F+APWC C+A P+ A+ Q+ ++ F +DCT +C + I +P FN +S EY D + F+ + NP +V L +F + + S +V FY+PWCG C A+ P + A L ++ + VD V +P + F N +
Sbjct: 463 EKEPWLVDFFAPWCPPCRALLPELRKASIQLF--GQLKFGTLDCTIHE--GLCNTYNIHAYPTTVIFN---KSSIHEYEGHHSADGILEFIEDLVNP---------------VVVTLGPESFQELVKRRKSSETWMVDFYAPWCGPCQALLPEWRRMARMLSGIVN---VGTVDCQKHHSFCQSESVRAYPEIRLFPQNSN 638
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Xenopus
Match: txndc5 (thioredoxin domain containing 5 [Source:NCBI gene;Acc:394794]) HSP 1 Score: 142.51 bits (358), Expect = 1.557e-36 Identity = 92/363 (25.34%), Postives = 165/363 (45.45%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQA--FNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFV-----HNPTQPPPQKDVPWSEETNEIDF----LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREED---DFVAFMTNPAN-------------PQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
MF+APWCG+C++++P + S + T D+ + GYP++ L + G + KY+G ++L ++ P +P ++ V + E+ LT ANF E++ + I F+APWCGHCKA P + A ++ + VDCT + +C+ + G+P +F G+ +Y +R+ D ++ PA PQ++ + + ++ L ++NF Q + + S + FY+PWCGHC + P + + + K + + IA VD T E L ++ V G+PTL+ F ++ G R + + F+++ D
Sbjct: 53 MFFAPWCGHCQRLQPTWNELGDKYNRMSKTPAYIAKVDCTTDMPTCTEHGVRGYPTLKLFRPGQEAVKYQGPRDLQSLENWMLQTLNDEPEKPKVEEKVEEPAKVPELKQGLYELTAANFKEHIAEGNHFI-KFFAPWCGHCKALAPAWEQLAATFQDSNSIKIAKVDCTQHN--GLCSDNQVRGYPTLLWFRNGEKVD--QYKGKRDLDSLKEYAESQLKPAEEKKEEEQKEDATPPQVE-----KPVAVESKVLSLSESNFDQTVATGVS--FIKFYAPWCGHCKNLVPIWEDLSKKEFSGMSDVKIAKVDCTAERALCNRFSVRGYPTLLLFRAGEKIGEHEGARDLETLQNFVLRHSRD 403 HSP 2 Score: 108.227 bits (269), Expect = 8.391e-25 Identity = 73/250 (29.20%), Postives = 122/250 (48.80%), Query Frame = 1
Query: 277 PWSEETNEIDF---LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKV-AFIG-VDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTN-----PANPQLDAR-RHWRRIPG-HEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWV-KYMGERTDDAITEF 987
PW E +E L A+ +++ + E +MF+APWCGHC+ +P + ++ K A+I VDCT + CT G+ G+P + F G+ +Y R+ +M P P+++ + ++P + + L NF ++I + F++PWCGHC A+ P++ A Q S IA VD T L + +V G+PTL++F NG+ V +Y G+R D++ E+
Sbjct: 21 PWEEGADEDPHKKNLYTADMFDHAIKQEPHFIMFFAPWCGHCQRLQPTWNELGDKYNRMSKTPAYIAKVDCTTD--MPTCTEHGVRGYPTLKLFRPGQEA--VKYQGPRDLQSLENWMLQTLNDEPEKPKVEEKVEEPAKVPELKQGLYELTAANFKEHIAE--GNHFIKFFAPWCGHCKALAPAWEQLAATFQD-SNSIKIAKVDCTQHNGLCSDNQVRGYPTLLWFR-NGEKVDQYKGKRDLDSLKEY 262 HSP 3 Score: 69.707 bits (169), Expect = 4.745e-12 Identity = 36/125 (28.80%), Postives = 64/125 (51.20%), Query Frame = 1
Query: 694 EKNNFVQNIVSHT---SGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPS-CLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKK 1056
+KN + ++ H +MF++PWCGHC ++P++ +K + + IA VD TT+ ++ V G+PTL F P + VKY G R ++ +M++ +D + E+ +E K
Sbjct: 32 KKNLYTADMFDHAIKQEPHFIMFFAPWCGHCQRLQPTWNELGDKYNRMSKTPAYIAKVDCTTDMPTCTEHGVRGYPTLKLFRPGQEAVKYQGPRDLQSLENWMLQTLNDEPEKPKVEEKVEEPAK 156
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Xenopus
Match: chst13 (carbohydrate (chondroitin 4) sulfotransferase 13 [Source:Xenbase;Acc:XB-GENE-945720]) HSP 1 Score: 136.732 bits (343), Expect = 1.871e-33 Identity = 81/255 (31.76%), Postives = 127/255 (49.80%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKK-VAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQE 1047
++ N + LTD NF ++ + V++ FYAPWCGHCK P++ A+ + +N + +D T+ + D+ I+G+P + G+ +Y R ++ V + A P W+ P EA ++L K+NF +VS LV FY+PWCGHC + P + AA +L P +A VDAT E+ L ++Y V+GFPTL F G Y G R I ++M ++ + +A K E
Sbjct: 25 KDENGVLVLTDKNFDTFITDKDIVLLEFYAPWCGHCKQFVPEYEKIASALNQNDPPIPVAKIDATEAT--DVAGRYDISGYPTIKILKKGQPI---DYDGARTQEAIVTKVKEIAQPD------WK--PPPEATIVLTKDNF-DEVVSDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVESSLGSKYGVTGFPTLKIFR-KGKVFDYNGPREKYGIVDYMTEQAGPPSKQIQAVKQVHE 264 HSP 2 Score: 121.324 bits (303), Expect = 2.507e-28 Identity = 69/212 (32.55%), Postives = 109/212 (51.42%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENK-KVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
FYAPWCG+CKQ P++ A+ L I + D + D+ ++I+GYP++ +++ G + Y+G ++EA++ V QP W LT NF E + + ++V FYAPWCGHCK P++ AA ++++ + VD T ES + + G+TGFP + F GK +Y+ RE+ V +MT A P
Sbjct: 52 FYAPWCGHCKQFVPEYEKIASALNQNDPPIPVAKIDATEATDVAGRYDISGYPTIKILKKGQPI-DYDGARTQEAIVTKVKEIAQPD------WKPPPEATIVLTKDNFDEVVSDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVES--SLGSKYGVTGFPTLKIFRKGKVF---DYNGPREKYGIVDYMTEQAGP 251 HSP 3 Score: 66.6254 bits (161), Expect = 6.673e-11 Identity = 46/161 (28.57%), Postives = 83/161 (51.55%), Query Frame = 1
Query: 556 NTSNSEYSLEREEDD-------FVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYMGERTD-DAITEFMIKKHS 1005
+ S +Y+ E EE D +AF P + ++ + G +V+ F Q ++ S L+ FY+PWCGHC +++P + + K ++ +IA +DAT + +YK GFPT +YF+P + +K+ G D +++++F I++HS
Sbjct: 446 DASGKKYAKEPEEFDSDGLREFVMAFKKGKLTPIIKSQPVPKNNKGPVKVVV--GKTFDQIVMDPKSDVLIEFYAPWCGHCKSLEPIYNDLGKKYRS-AEGLIIAKMDATANDITSDKYKAEGFPT-IYFAPRNNKQNPIKFSGGNRDLESLSKF-IEEHS 601 HSP 4 Score: 63.1586 bits (152), Expect = 8.696e-10 Identity = 29/90 (32.22%), Postives = 50/90 (55.56%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQK 270
FYAPWCG+CK++ P++ AA L +S I + D + L + +TG+P++ + + G KVF Y G K ++ ++ PP ++
Sbjct: 167 FYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVESSLGSKYGVTGFPTLKIFRKG-KVFDYNGPREKYGIVDYMTEQAGPPSKQ 255
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Xenopus
Match: chst13 (carbohydrate (chondroitin 4) sulfotransferase 13 [Source:Xenbase;Acc:XB-GENE-945720]) HSP 1 Score: 136.732 bits (343), Expect = 2.129e-33 Identity = 81/255 (31.76%), Postives = 127/255 (49.80%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKK-VAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQE 1047
++ N + LTD NF ++ + V++ FYAPWCGHCK P++ A+ + +N + +D T+ + D+ I+G+P + G+ +Y R ++ V + A P W+ P EA ++L K+NF +VS LV FY+PWCGHC + P + AA +L P +A VDAT E+ L ++Y V+GFPTL F G Y G R I ++M ++ + +A K E
Sbjct: 44 KDENGVLVLTDKNFDTFITDKDIVLLEFYAPWCGHCKQFVPEYEKIASALNQNDPPIPVAKIDATEAT--DVAGRYDISGYPTIKILKKGQPI---DYDGARTQEAIVTKVKEIAQPD------WK--PPPEATIVLTKDNF-DEVVSDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVESSLGSKYGVTGFPTLKIFR-KGKVFDYNGPREKYGIVDYMTEQAGPPSKQIQAVKQVHE 283 HSP 2 Score: 121.324 bits (303), Expect = 2.432e-28 Identity = 69/212 (32.55%), Postives = 109/212 (51.42%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENK-KVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
FYAPWCG+CKQ P++ A+ L I + D + D+ ++I+GYP++ +++ G + Y+G ++EA++ V QP W LT NF E + + ++V FYAPWCGHCK P++ AA ++++ + VD T ES + + G+TGFP + F GK +Y+ RE+ V +MT A P
Sbjct: 71 FYAPWCGHCKQFVPEYEKIASALNQNDPPIPVAKIDATEATDVAGRYDISGYPTIKILKKGQPI-DYDGARTQEAIVTKVKEIAQP------DWKPPPEATIVLTKDNFDEVVSDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVES--SLGSKYGVTGFPTLKIFRKGKVF---DYNGPREKYGIVDYMTEQAGP 270 HSP 3 Score: 66.6254 bits (161), Expect = 5.990e-11 Identity = 46/161 (28.57%), Postives = 83/161 (51.55%), Query Frame = 1
Query: 556 NTSNSEYSLEREEDD-------FVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYMGERTD-DAITEFMIKKHS 1005
+ S +Y+ E EE D +AF P + ++ + G +V+ F Q ++ S L+ FY+PWCGHC +++P + + K ++ +IA +DAT + +YK GFPT +YF+P + +K+ G D +++++F I++HS
Sbjct: 465 DASGKKYAKEPEEFDSDGLREFVMAFKKGKLTPIIKSQPVPKNNKGPVKVVV--GKTFDQIVMDPKSDVLIEFYAPWCGHCKSLEPIYNDLGKKYRS-AEGLIIAKMDATANDITSDKYKAEGFPT-IYFAPRNNKQNPIKFSGGNRDLESLSKF-IEEHS 620 HSP 4 Score: 63.1586 bits (152), Expect = 7.996e-10 Identity = 29/90 (32.22%), Postives = 50/90 (55.56%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQK 270
FYAPWCG+CK++ P++ AA L +S I + D + L + +TG+P++ + + G KVF Y G K ++ ++ PP ++
Sbjct: 186 FYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVESSLGSKYGVTGFPTLKIFRKG-KVFDYNGPREKYGIVDYMTEQAGPPSKQ 274
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Xenopus
Match: chst13 (carbohydrate (chondroitin 4) sulfotransferase 13 [Source:Xenbase;Acc:XB-GENE-945720]) HSP 1 Score: 136.346 bits (342), Expect = 2.287e-33 Identity = 81/255 (31.76%), Postives = 127/255 (49.80%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKK-VAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQE 1047
++ N + LTD NF ++ + V++ FYAPWCGHCK P++ A+ + +N + +D T+ + D+ I+G+P + G+ +Y R ++ V + A P W+ P EA ++L K+NF +VS LV FY+PWCGHC + P + AA +L P +A VDAT E+ L ++Y V+GFPTL F G Y G R I ++M ++ + +A K E
Sbjct: 27 KDENGVLVLTDKNFDTFITDKDIVLLEFYAPWCGHCKQFVPEYEKIASALNQNDPPIPVAKIDATEAT--DVAGRYDISGYPTIKILKKGQPI---DYDGARTQEAIVTKVKEIAQPD------WK--PPPEATIVLTKDNF-DEVVSDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVESSLGSKYGVTGFPTLKIFR-KGKVFDYNGPREKYGIVDYMTEQAGPPSKQIQAVKQVHE 266 HSP 2 Score: 121.324 bits (303), Expect = 2.625e-28 Identity = 69/212 (32.55%), Postives = 109/212 (51.42%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENK-KVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
FYAPWCG+CKQ P++ A+ L I + D + D+ ++I+GYP++ +++ G + Y+G ++EA++ V QP W LT NF E + + ++V FYAPWCGHCK P++ AA ++++ + VD T ES + + G+TGFP + F GK +Y+ RE+ V +MT A P
Sbjct: 54 FYAPWCGHCKQFVPEYEKIASALNQNDPPIPVAKIDATEATDVAGRYDISGYPTIKILKKGQPI-DYDGARTQEAIVTKVKEIAQP------DWKPPPEATIVLTKDNFDEVVSDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVES--SLGSKYGVTGFPTLKIFRKGKVF---DYNGPREKYGIVDYMTEQAGP 253 HSP 3 Score: 66.6254 bits (161), Expect = 6.461e-11 Identity = 46/161 (28.57%), Postives = 83/161 (51.55%), Query Frame = 1
Query: 556 NTSNSEYSLEREEDD-------FVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYMGERTD-DAITEFMIKKHS 1005
+ S +Y+ E EE D +AF P + ++ + G +V+ F Q ++ S L+ FY+PWCGHC +++P + + K ++ +IA +DAT + +YK GFPT +YF+P + +K+ G D +++++F I++HS
Sbjct: 448 DASGKKYAKEPEEFDSDGLREFVMAFKKGKLTPIIKSQPVPKNNKGPVKVVV--GKTFDQIVMDPKSDVLIEFYAPWCGHCKSLEPIYNDLGKKYRS-AEGLIIAKMDATANDITSDKYKAEGFPT-IYFAPRNNKQNPIKFSGGNRDLESLSKF-IEEHS 603 HSP 4 Score: 63.1586 bits (152), Expect = 9.260e-10 Identity = 29/90 (32.22%), Postives = 50/90 (55.56%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQK 270
FYAPWCG+CK++ P++ AA L +S I + D + L + +TG+P++ + + G KVF Y G K ++ ++ PP ++
Sbjct: 169 FYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVESSLGSKYGVTGFPTLKIFRKG-KVFDYNGPREKYGIVDYMTEQAGPPSKQ 257
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Xenopus
Match: chst13 (carbohydrate (chondroitin 4) sulfotransferase 13 [Source:Xenbase;Acc:XB-GENE-945720]) HSP 1 Score: 136.346 bits (342), Expect = 2.289e-33 Identity = 81/255 (31.76%), Postives = 127/255 (49.80%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKK-VAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQE 1047
++ N + LTD NF ++ + V++ FYAPWCGHCK P++ A+ + +N + +D T+ + D+ I+G+P + G+ +Y R ++ V + A P W+ P EA ++L K+NF +VS LV FY+PWCGHC + P + AA +L P +A VDAT E+ L ++Y V+GFPTL F G Y G R I ++M ++ + +A K E
Sbjct: 47 KDENGVLVLTDKNFDTFITDKDIVLLEFYAPWCGHCKQFVPEYEKIASALNQNDPPIPVAKIDATEAT--DVAGRYDISGYPTIKILKKGQPI---DYDGARTQEAIVTKVKEIAQPD------WK--PPPEATIVLTKDNF-DEVVSDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVESSLGSKYGVTGFPTLKIFR-KGKVFDYNGPREKYGIVDYMTEQAGPPSKQIQAVKQVHE 286 HSP 2 Score: 121.324 bits (303), Expect = 2.840e-28 Identity = 69/212 (32.55%), Postives = 109/212 (51.42%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENK-KVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
FYAPWCG+CKQ P++ A+ L I + D + D+ ++I+GYP++ +++ G + Y+G ++EA++ V QP W LT NF E + + ++V FYAPWCGHCK P++ AA ++++ + VD T ES + + G+TGFP + F GK +Y+ RE+ V +MT A P
Sbjct: 74 FYAPWCGHCKQFVPEYEKIASALNQNDPPIPVAKIDATEATDVAGRYDISGYPTIKILKKGQPI-DYDGARTQEAIVTKVKEIAQPD------WKPPPEATIVLTKDNFDEVVSDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVES--SLGSKYGVTGFPTLKIFRKGKVF---DYNGPREKYGIVDYMTEQAGP 273 HSP 3 Score: 66.6254 bits (161), Expect = 6.895e-11 Identity = 46/161 (28.57%), Postives = 83/161 (51.55%), Query Frame = 1
Query: 556 NTSNSEYSLEREEDD-------FVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYMGERTD-DAITEFMIKKHS 1005
+ S +Y+ E EE D +AF P + ++ + G +V+ F Q ++ S L+ FY+PWCGHC +++P + + K ++ +IA +DAT + +YK GFPT +YF+P + +K+ G D +++++F I++HS
Sbjct: 459 DASGKKYAKEPEEFDSDGLREFVMAFKKGKLTPIIKSQPVPKNNKGPVKVVV--GKTFDQIVMDPKSDVLIEFYAPWCGHCKSLEPIYNDLGKKYRS-AEGLIIAKMDATANDITSDKYKAEGFPT-IYFAPRNNKQNPIKFSGGNRDLESLSKF-IEEHS 614 HSP 4 Score: 63.1586 bits (152), Expect = 8.666e-10 Identity = 29/90 (32.22%), Postives = 50/90 (55.56%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQK 270
FYAPWCG+CK++ P++ AA L +S I + D + L + +TG+P++ + + G KVF Y G K ++ ++ PP ++
Sbjct: 189 FYAPWCGHCKKLAPEYEKAAQELSKRSPPIPLAKVDATVESSLGSKYGVTGFPTLKIFRKG-KVFDYNGPREKYGIVDYMTEQAGPPSKQ 277
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Mouse
Match: Pdia5 (protein disulfide isomerase associated 5 [Source:MGI Symbol;Acc:MGI:1919849]) HSP 1 Score: 184.496 bits (467), Expect = 6.815e-51 Identity = 116/351 (33.05%), Postives = 182/351 (51.85%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEG-DNSKEALIKFVHNPTQPPPQ-KDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM---AENKKVAFIGVDCT-DESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETH--LAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM-IKKHSDIIRHEE 1026
MFYAPWC CK++ P F AAT ++G + G+N S +++K+ +N+ GYP++ + G +F YE ++ E +++++ NP P PQ + PW++E + LTD +F +++ +H SV+VMF+APWCGHCK KP+F SAA + AE+ V VD T +E+ A I+ FP +YF KN R + F+ +M NP P + +++ L +NF ++ + LVMFY+PWC HC + P F A+ + AAVD + + L Q V +PT Y+ KY +RT+ T F+ + D+ R E+
Sbjct: 174 MFYAPWCSMCKRIMPHFQKAATQVRGHIVLAGMNVYPSEFENIKEEYNVRGYPTICYFEKGRFLFPYENYGSTAEDIVEWLKNPLPPQPQVPETPWADEGGSVYHLTDEDFDQFVKEHSSVLVMFHAPWCGHCKKMKPEFESAAEVLHGDAESSGV-LAAVDATVNEALAG---RFHISAFPTLKYF---KNGEQQAVPALRTKKKFIEWMQNPEAPPPPEPTWEEQ---QTSVLHLVGDNF-RDTLKKKKHTLVMFYAPWCPHCKKVIPHFTATADAFKEDR-KIACAAVDCVKDKNQDLCQQEAVKAYPTFHYYHYGKLVEKYESDRTELGFTSFIRTLREGDLKRLEK 512 HSP 2 Score: 137.502 bits (345), Expect = 2.829e-34 Identity = 95/322 (29.50%), Postives = 154/322 (47.83%), Query Frame = 1
Query: 49 FAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPS-----VFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHES-VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDA-RRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQT-LLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+ A +KG+ ++ + + L + + P +F QDG +Y+ + ++++ F+ +P PP ++ P +++ ID ++ +F L + E +++MFYAPWC CK P F AA Q+ + + G++ F +I + G+P YF G+ E + +D V ++ NP PQ W G ++ L +F Q + H+S LVMF++PWCGHC MKP F +AA L S ++AAVDAT LA ++ +S FPTL YF NG+ RT E+M
Sbjct: 65 LSTVAQAVKGQGTVCWVDCGDAESRKLCKKMKVDLSPKDKKIELFHYQDGAFHMQYDRAVTLKSIVAFLKDPKGPPLWEEDPGAKDVVHID--SEKDFRRLLKREEKPLLMMFYAPWCSMCKRIMPHFQKAATQVRGH--IVLAGMNVYPSEFENIKEEYNVRGYPTICYFEKGRFLFPYE-NYGSTAEDIVEWLKNPLPPQPQVPETPWADEGG--SVYHLTDEDFDQFVKEHSS-VLVMFHAPWCGHCKKMKPEFESAAEVLHGDAESSGVLAAVDATVNEALAGRFHISAFPTLKYFK-NGEQQAVPALRTKKKFIEWM 377
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Mouse
Match: Txndc5 (thioredoxin domain containing 5 [Source:MGI Symbol;Acc:MGI:2145316]) HSP 1 Score: 148.288 bits (373), Expect = 1.154e-38 Identity = 94/352 (26.70%), Postives = 167/352 (47.44%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQF---AAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFV-----HNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREE-DDFVA--FMTNPANPQL----DARRHWRRIPGHEAIVI-LEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
MF+APWCG+C++++P + +++ +++ D + D+ A + GYP++ + G + KY+G E L ++ P P P+ + P + E + + AN +E + + F+APWCGHCKA P + A + ++ V VDCT + +A +C+ + G+P +F GK + + E D+V + A P+ +A G + V+ L + +F I + V FY+PWCGHC + P++ + K L IA VD T E ++ ++Y V G+PTL+ F ++ G R D++ F++++ D
Sbjct: 69 MFFAPWCGHCQRLQPTWNDLGDKYNSMEDAKVYVA-KVDCTADSDVCSAQGVRGYPTLKFFKPGQEAVKYQGPRDFETLENWMLQTLNEEPATPEPEAEPPRAPELKQGLYELSANNFELHVSQGNHFIKFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCT-QHYA-VCSEHQVRGYPTLLWFRDGKKVDQYKGKRDLESLRDYVQSQLQGSEAAPETVEPSEAPVMAAEPTGDKGTVLALTEKSFEDTIAQGIT--FVKFYAPWCGHCKNLAPTWEELSKKEFPGLSDVTIAEVDCTAERNVCSKYSVRGYPTLLLFRGGEKVGEHNGGRDLDSLHSFVLRQAKD 415 HSP 2 Score: 111.694 bits (278), Expect = 5.643e-26 Identity = 67/243 (27.57%), Postives = 123/243 (50.62%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM--AENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTN-----PANPQLDARRHWRRIPG-HEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
E+ L A+ + + Q + VMF+APWCGHC+ +P + ++ E+ KV VDCT +S D+C+++G+ G+P ++F G+ +Y R+ + +M PA P+ +A R P + + L NNF ++ + F++PWCGHC A+ P++ A L+ + I VD T + ++++V G+PTL++F +Y G+R +++ +++
Sbjct: 43 EQDPHAKHLYTADMFTHGIQSAAHFVMFFAPWCGHCQRLQPTWNDLGDKYNSMEDAKVYVAKVDCTADS--DVCSAQGVRGYPTLKFFKPGQEA--VKYQGPRDFETLENWMLQTLNEEPATPEPEAEP--PRAPELKQGLYELSANNFELHVSQ--GNHFIKFFAPWCGHCKALAPTWEQLALGLEH-SETVKIGKVDCTQHYAVCSEHQVRGYPTLLWFRDGKKVDQYKGKRDLESLRDYV 276
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Mouse
Match: Txndc5 (thioredoxin domain containing 5 [Source:MGI Symbol;Acc:MGI:2145316]) HSP 1 Score: 134.42 bits (337), Expect = 2.370e-34 Identity = 90/348 (25.86%), Postives = 164/348 (47.13%), Query Frame = 1
Query: 19 CGYCKQMKPQF---AAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFV-----HNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREED---DFVA--FMTNPANPQL----DARRHWRRIPGHEAIVI-LEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
CG+C++++P + +++ +++ D + D+ A + GYP++ + G + KY+G E L ++ P P P+ + P + E + + AN +E + + F+APWCGHCKA P + A + ++ V VDCT + +A +C+ + G+P +F GK +Y +R+ + D+V + A P+ +A G + V+ L + +F I + V FY+PWCGHC + P++ + K L IA VD T E ++ ++Y V G+PTL+ F ++ G R D++ F++++ D
Sbjct: 2 CGHCQRLQPTWNDLGDKYNSMEDAKVYVA-KVDCTADSDVCSAQGVRGYPTLKFFKPGQEAVKYQGPRDFETLENWMLQTLNEEPATPEPEAEPPRAPELKQGLYELSANNFELHVSQGNHFIKFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCT-QHYA-VCSEHQVRGYPTLLWFRDGKKVD--QYKGKRDLESLRDYVQSQLQGSEAAPETVEPSEAPVMAAEPTGDKGTVLALTEKSFEDTIAQGIT--FVKFYAPWCGHCKNLAPTWEELSKKEFPGLSDVTIAEVDCTAERNVCSKYSVRGYPTLLLFRGGEKVGEHNGGRDLDSLHSFVLRQAKD 342 HSP 2 Score: 92.0485 bits (227), Expect = 1.057e-19 Identity = 64/226 (28.32%), Postives = 106/226 (46.90%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLK-GKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQ---------PPPQKDVPWSEETNE---IDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTS-AANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQL 639
F+APWCG+CK + P + A L+ +++ IG D + + + GYP++ +DG KV +Y+G E+L +V + Q P + V +E T + + LT+ +F + + Q V FYAPWCGHCK P + + + V VDCT E ++C+ + G+P F G+ E++ R+ D +F+ A +L
Sbjct: 125 FFAPWCGHCKALAPTWEQLALGLEHSETVKIG-KVDCTQHYAVCSEHQVRGYPTLLWFRDGKKVDQYKGKRDLESLRDYVQSQLQGSEAAPETVEPSEAPVMAAEPTGDKGTVLALTEKSFEDTIAQG-ITFVKFYAPWCGHCKNLAPTWEELSKKEFPGLSDVTIAEVDCTAER--NVCSKYSVRGYPTLLLFRGGEKV--GEHNGGRDLDSLHSFVLRQAKDEL 344
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Mouse
Match: Txndc5 (thioredoxin domain containing 5 [Source:MGI Symbol;Acc:MGI:2145316]) HSP 1 Score: 128.257 bits (321), Expect = 2.198e-32 Identity = 85/314 (27.07%), Postives = 145/314 (46.18%), Query Frame = 1
Query: 106 DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFV-----HNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREE-DDFVA--FMTNPANPQL----DARRHWRRIPGHEAIVI-LEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
D + D+ A + GYP++ + G + KY+G E L ++ P P P+ + P + E + + AN +E + + F+APWCGHCKA P + A + ++ V VDCT + +A +C+ + G+P +F GK + + E D+V + A P+ +A G + V+ L + +F I + V FY+PWCGHC + P++ + K L IA VD T E ++ ++Y V G+PTL+ F ++ G R D++ F++++ D
Sbjct: 12 DCTADSDVCSAQGVRGYPTLKFFKPGQEAVKYQGPRDFETLENWMLQTLNEEPATPEPEAEPPRAPELKQGLYELSANNFELHVSQGNHFIKFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCT-QHYA-VCSEHQVRGYPTLLWFRDGKKVDQYKGKRDLESLRDYVQSQLQGSEAAPETVEPSEAPVMAAEPTGDKGTVLALTEKSFEDTIAQGIT--FVKFYAPWCGHCKNLAPTWEELSKKEFPGLSDVTIAEVDCTAERNVCSKYSVRGYPTLLLFRGGEKVGEHNGGRDLDSLHSFVLRQAKD 321 HSP 2 Score: 92.0485 bits (227), Expect = 6.483e-20 Identity = 64/226 (28.32%), Postives = 106/226 (46.90%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLK-GKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQ---------PPPQKDVPWSEETNE---IDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTS-AANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQL 639
F+APWCG+CK + P + A L+ +++ IG D + + + GYP++ +DG KV +Y+G E+L +V + Q P + V +E T + + LT+ +F + + Q V FYAPWCGHCK P + + + V VDCT E ++C+ + G+P F G+ E++ R+ D +F+ A +L
Sbjct: 104 FFAPWCGHCKALAPTWEQLALGLEHSETVKIG-KVDCTQHYAVCSEHQVRGYPTLLWFRDGKKVDQYKGKRDLESLRDYVQSQLQGSEAAPETVEPSEAPVMAAEPTGDKGTVLALTEKSFEDTIAQG-ITFVKFYAPWCGHCKNLAPTWEELSKKEFPGLSDVTIAEVDCTAER--NVCSKYSVRGYPTLLLFRGGEKV--GEHNGGRDLDSLHSFVLRQAKDEL 323
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Mouse
Match: Pdia4 (protein disulfide isomerase associated 4 [Source:MGI Symbol;Acc:MGI:104864]) HSP 1 Score: 132.88 bits (333), Expect = 3.466e-32 Identity = 76/236 (32.20%), Postives = 122/236 (51.69%), Query Frame = 1
Query: 295 NEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKK-VAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKK 999
N + L D NF ++ ++V++ FYAPWCGHCK P++ A+ + +N +A +D T S + + ++G+P + G+ +Y R +++ VA + + P W P E + L K+NF ++V++ LV FY+PWCGHC + P + AA +L P +A VDAT +T LA ++ VSG+PTL F G Y G R I ++MI++
Sbjct: 58 NGVWVLNDGNFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASM--LASKFDVSGYPTIKILKKGQAV---DYDGSRTQEEIVAKVREVSQPD------W--TPPPEVTLSLTKDNF-DDVVNNADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATEQTDLAKRFDVSGYPTLKIFR-KGRPFDYNGPREKYGIVDYMIEQ 278 HSP 2 Score: 117.857 bits (294), Expect = 2.832e-27 Identity = 63/212 (29.72%), Postives = 113/212 (53.30%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAE-NKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
FYAPWCG+CKQ P++ A+TLK I + D ++ L F+++GYP++ +++ G V Y+G ++E ++ V +QP W+ LT NF + ++ + ++V FYAPWCGHCK P++ AA ++++ + + VD T+++ D+ ++G+P + F G+ +Y+ RE+ V +M + P
Sbjct: 82 FYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASMLASKFDVSGYPTIKILKKGQAV-DYDGSRTQEEIVAKVREVSQP------DWTPPPEVTLSLTKDNFDDVVNNADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATEQT--DLAKRFDVSGYPTLKIFRKGRPF---DYNGPREKYGIVDYMIEQSGP 281 HSP 3 Score: 65.4698 bits (158), Expect = 1.066e-10 Identity = 45/148 (30.41%), Postives = 72/148 (48.65%), Query Frame = 1
Query: 556 NTSNSEYSLEREEDD------FV-AFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYMGERTD 969
+ S ++++E EE D FV AF P + ++ + G +V+ F ++ L+ FY+PWCGHC ++P + + K + +IA +DAT QYKV GFPT +YF+P+GD +K+ G D
Sbjct: 476 DESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVV--GKTFDAIVMDPKKDVLIEFYAPWCGHCKQLEPIYTSLGKKYKG-QKDLVIAKMDATANDITNDQYKVEGFPT-IYFAPSGDKKNPIKFEGGNRD 619 HSP 4 Score: 59.3066 bits (142), Expect = 1.019e-8 Identity = 27/82 (32.93%), Postives = 44/82 (53.66%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKV--FKYEGDNSK-EALIKFV 240
FYAPWCG+CKQ++P + + KG+ + D + D + + G+P+++ G+K K+EG N E L KF+
Sbjct: 546 FYAPWCGHCKQLEPIYTSLGKKYKGQKDLVIAKMDATANDITNDQYKVEGFPTIYFAPSGDKKNPIKFEGGNRDLEHLSKFI 627 HSP 5 Score: 49.6766 bits (117), Expect = 9.293e-6 Identity = 24/73 (32.88%), Postives = 36/73 (49.32%), Query Frame = 1
Query: 337 LDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSE-GITGFPIFRYFNYG 552
+D + V++ FYAPWCGHCK +P +TS + K + +D T DI + + GFP + G
Sbjct: 536 MDPKKDVLIEFYAPWCGHCKQLEPIYTSLGKKYKGQKDLVIAKMDATAN---DITNDQYKVEGFPTIYFAPSG 605
BLAST of Protein disulfide-isomerase A5 vs. UniProt/SwissProt
Match: sp|Q921X9|PDIA5_MOUSE (Protein disulfide-isomerase A5 OS=Mus musculus OX=10090 GN=Pdia5 PE=1 SV=1) HSP 1 Score: 184.496 bits (467), Expect = 4.767e-50 Identity = 116/351 (33.05%), Postives = 182/351 (51.85%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEG-DNSKEALIKFVHNPTQPPPQ-KDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM---AENKKVAFIGVDCT-DESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETH--LAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM-IKKHSDIIRHEE 1026
MFYAPWC CK++ P F AAT ++G + G+N S +++K+ +N+ GYP++ + G +F YE ++ E +++++ NP P PQ + PW++E + LTD +F +++ +H SV+VMF+APWCGHCK KP+F SAA + AE+ V VD T +E+ A I+ FP +YF KN R + F+ +M NP P + +++ L +NF ++ + LVMFY+PWC HC + P F A+ + AAVD + + L Q V +PT Y+ KY +RT+ T F+ + D+ R E+
Sbjct: 174 MFYAPWCSMCKRIMPHFQKAATQVRGHIVLAGMNVYPSEFENIKEEYNVRGYPTICYFEKGRFLFPYENYGSTAEDIVEWLKNPLPPQPQVPETPWADEGGSVYHLTDEDFDQFVKEHSSVLVMFHAPWCGHCKKMKPEFESAAEVLHGDAESSGV-LAAVDATVNEALAG---RFHISAFPTLKYF---KNGEQQAVPALRTKKKFIEWMQNPEAPPPPEPTWEEQ---QTSVLHLVGDNF-RDTLKKKKHTLVMFYAPWCPHCKKVIPHFTATADAFKEDR-KIACAAVDCVKDKNQDLCQQEAVKAYPTFHYYHYGKLVEKYESDRTELGFTSFIRTLREGDLKRLEK 512 HSP 2 Score: 137.502 bits (345), Expect = 1.979e-33 Identity = 95/322 (29.50%), Postives = 154/322 (47.83%), Query Frame = 1
Query: 49 FAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPS-----VFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHES-VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDA-RRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQT-LLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+ A +KG+ ++ + + L + + P +F QDG +Y+ + ++++ F+ +P PP ++ P +++ ID ++ +F L + E +++MFYAPWC CK P F AA Q+ + + G++ F +I + G+P YF G+ E + +D V ++ NP PQ W G ++ L +F Q + H+S LVMF++PWCGHC MKP F +AA L S ++AAVDAT LA ++ +S FPTL YF NG+ RT E+M
Sbjct: 65 LSTVAQAVKGQGTVCWVDCGDAESRKLCKKMKVDLSPKDKKIELFHYQDGAFHMQYDRAVTLKSIVAFLKDPKGPPLWEEDPGAKDVVHID--SEKDFRRLLKREEKPLLMMFYAPWCSMCKRIMPHFQKAATQVRGH--IVLAGMNVYPSEFENIKEEYNVRGYPTICYFEKGRFLFPYE-NYGSTAEDIVEWLKNPLPPQPQVPETPWADEGG--SVYHLTDEDFDQFVKEHSS-VLVMFHAPWCGHCKKMKPEFESAAEVLHGDAESSGVLAAVDATVNEALAGRFHISAFPTLKYFK-NGEQQAVPALRTKKKFIEWM 377
BLAST of Protein disulfide-isomerase A5 vs. UniProt/SwissProt
Match: sp|Q5I0H9|PDIA5_RAT (Protein disulfide-isomerase A5 OS=Rattus norvegicus OX=10116 GN=Pdia5 PE=2 SV=1) HSP 1 Score: 173.326 bits (438), Expect = 5.712e-46 Identity = 115/351 (32.76%), Postives = 183/351 (52.14%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEG-DNSKEALIKFVHNPTQPPPQK-DVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM---AENKKVAFIGVDCT-DESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETH--LAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM-IKKHSDIIRHEE 1026
MFYAPWC CK++ P F AAT ++G ++ G+N +++K+ +N+ GYP++ + G +F+YE ++ E +++++ NP P PQ + PW++E + LTD +F +++ +H SV+VMF+APWCGHCK KP+F SAA + AE+ V VD T +E+ A+ I+ FP +YF KN R + F+ +M NP P + +++ L +NF + + LVMFY+PWC HC + P F A+ + AAVD + + L Q V +PT Y+ KY +RT+ T F+ + D+ R E+
Sbjct: 174 MFYAPWCSMCKRIMPHFQKAATQVRGHTVLAGMNVYPPEFENIKEEYNVRGYPTICYFEKGRFLFQYENYGSTAEDIVEWLKNPQPPQPQVPETPWADEGGSVYHLTDEDFDQFVKEHSSVLVMFHAPWCGHCKKMKPEFESAAEVLHGDAESSGV-LAAVDATINEALAE---RFHISAFPTLKYF---KNGEQQAVPALRTKKKFIEWMQNPEAPPPPEPTWEEQ---QTSVLHLVGDNFRETL-KKKKHTLVMFYAPWCPHCKKVIPHFTATADAFKDDR-KIACAAVDCVKDKNQDLCQQESVKAYPTFHYYHYGKLVEKYESDRTELGFTSFIRTLREGDLKRLEK 512 HSP 2 Score: 129.028 bits (323), Expect = 1.836e-30 Identity = 96/322 (29.81%), Postives = 155/322 (48.14%), Query Frame = 1
Query: 49 FAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPS-----VFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHES-VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRH-WRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQT-LLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+ A +KG+ ++ + + L + + P +F QDG +Y+ + ++++ F+ +P PP ++ P +++ ID ++ +F L + E +++MFYAPWC CK P F AA Q+ + +A G++ F +I + G+P YF G+ E + +D V ++ NP PQ W G ++ L +F Q + H+S LVMF++PWCGHC MKP F +AA L S ++AAVDAT LA ++ +S FPTL YF NG+ RT E+M
Sbjct: 65 LSTVAQAVKGQGTICWVDCGDAESRKLCKKMKVDLSPKDKKIELFHYQDGAFHMQYDRAVTLKSIVAFLKDPKGPPLWEEDPGAKDVVHID--SEKDFRRLLKKEEKPLLMMFYAPWCSMCKRIMPHFQKAATQVRGHTVLA--GMNVYPPEFENIKEEYNVRGYPTICYFEKGRFLFQYE-NYGSTAEDIVEWLKNPQPPQPQVPETPWADEGG--SVYHLTDEDFDQFVKEHSS-VLVMFHAPWCGHCKKMKPEFESAAEVLHGDAESSGVLAAVDATINEALAERFHISAFPTLKYFK-NGEQQAVPALRTKKKFIEWM 377
BLAST of Protein disulfide-isomerase A5 vs. UniProt/SwissProt
Match: sp|Q14554|PDIA5_HUMAN (Protein disulfide-isomerase A5 OS=Homo sapiens OX=9606 GN=PDIA5 PE=1 SV=1) HSP 1 Score: 167.933 bits (424), Expect = 4.561e-44 Identity = 109/338 (32.25%), Postives = 179/338 (52.96%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEG-DNSKEALIKFVHNPTQPPPQK-DVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM---AENKKVAFIGVDCT-DESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETH--LAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWC CK+M P F AAT L+G ++ G+N S +++K+ +++ G+P++ + G +F+Y+ ++ E +++++ NP P PQ + PW++E + LTD +F +++ +H SV+VMF+APWCGHCK KP+F AA + A++ V VD T +++ A+ I+ FP +YF KN + R + F+ +M NP P + +++ L +NF + + LVMFY+PWC HC + P F A+ + AAVD + + L Q V G+PT Y+ KY +RT+ T ++
Sbjct: 176 MFYAPWCSMCKRMMPHFQKAATQLRGHAVLAGMNVYSSEFENIKEEYSVRGFPTICYFEKGRFLFQYDNYGSTAEDIVEWLKNPQPPQPQVPETPWADEGGSVYHLTDEDFDQFVKEHSSVLVMFHAPWCGHCKKMKPEFEKAAEALHGEADSSGV-LAAVDATVNKALAE---RFHISEFPTLKYF---KNGEKYAVPVLRTKKKFLEWMQNPEAPPPPEPTWEEQ---QTSVLHLVGDNF-RETLKKKKHTLVMFYAPWCPHCKKVIPHFTATADAFKDDR-KIACAAVDCVKDKNQDLCQQEAVKGYPTFHYYHYGKFAEKYDSDRTELGFTNYI 501 HSP 2 Score: 126.331 bits (316), Expect = 1.556e-29 Identity = 95/322 (29.50%), Postives = 153/322 (47.52%), Query Frame = 1
Query: 49 FAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPS-----VFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHES-VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRH-WRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSC-LIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+ A +KG+ ++ + + L + + P +F QDG +Y + ++++ F+ +P PP ++ P +++ +D ++ +F L + E +++MFYAPWC CK P F AA Q+ + +A G++ F +I + GFP YF G+ + + +D V ++ NP PQ W G ++ L +F Q + H+S LVMF++PWCGHC MKP F AA L S ++AAVDAT LA ++ +S FPTL YF NG+ RT E+M
Sbjct: 67 LSTVAQAVKGQGTICWVDCGDAESRKLCKKMKVDLSPKDKKVELFHYQDGAFHTEYNRAVTFKSIVAFLKDPKGPPLWEEDPGAKDVVHLD--SEKDFRRLLKKEEKPLLIMFYAPWCSMCKRMMPHFQKAATQLRGHAVLA--GMNVYSSEFENIKEEYSVRGFPTICYFEKGRFLFQYD-NYGSTAEDIVEWLKNPQPPQPQVPETPWADEGG--SVYHLTDEDFDQFVKEHSS-VLVMFHAPWCGHCKKMKPEFEKAAEALHGEADSSGVLAAVDATVNKALAERFHISEFPTLKYFK-NGEKYAVPVLRTKKKFLEWM 379
BLAST of Protein disulfide-isomerase A5 vs. UniProt/SwissProt
Match: sp|Q2KIL5|PDIA5_BOVIN (Protein disulfide-isomerase A5 OS=Bos taurus OX=9913 GN=PDIA5 PE=2 SV=1) HSP 1 Score: 164.466 bits (415), Expect = 7.952e-43 Identity = 107/337 (31.75%), Postives = 171/337 (50.74%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEG-DNSKEALIKFVHNPTQPPPQK-DVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM--AENKKVAFIGVDCT-DESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETH--LAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWC CK++ P F AAT L+G+ + G+N S +++K+ +++ GYP++ + G +F+Y+ ++ E +++++ NP P PQ + PW++E + L+D +F +++ +H SV+VMF+APWCGHCK KP+F SAA + + VD T +++ A+ I FP +YF KN R + F+ +M N W +++ L +NF + + ALVMFY+PWC HC P F AA+ + AA+D E + L Q V +PT Y+ KY T+ T F+
Sbjct: 178 MFYAPWCSVCKRIMPHFQKAATQLRGQFVLAGMNVYPSEFENIKEEYSVRGYPTICYFEKGRFLFQYDSYGSTAEDIVEWLKNPQPPQPQVPETPWADEGGSVYHLSDEDFDQFVKEHSSVLVMFHAPWCGHCKKMKPEFESAAEVLHGEGDSSGVLAAVDATVNKALAE---RFHIAEFPTLKYF---KNGEKYAVPALRTKKSFIEWMRN-PESPPPPDPAWEE--QQTSVLHLSGDNFRETL-KRKKHALVMFYAPWCPHCKKAIPHFTAAADAFKDDR-KIACAAIDCVKENNKDLCQQEAVKAYPTFHYYHYGKFVEKYDTNPTELGFTSFI 503 HSP 2 Score: 124.02 bits (310), Expect = 9.765e-29 Identity = 89/279 (31.90%), Postives = 138/279 (49.46%), Query Frame = 1
Query: 163 VFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHES-VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRH-WRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSC-LIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+F QDG +Y + ++++ F+ +P PP ++ P +++ ID + +F L + E +++MFYAPWC CK P F AA Q+ + G++ F +I + G+P YF G+ + S +D V ++ NP PQ W G ++ L +F Q + H+S LVMF++PWCGHC MKP F +AA L S ++AAVDAT LA ++ ++ FPTL YF NG+ RT + E+M
Sbjct: 112 LFHYQDGAFHTEYNRAVTFKSIVAFLKDPKGPPLWEEDPGAKDVVHID--NEKDFRRLLKKEEKPILMMFYAPWCSVCKRIMPHFQKAATQL--RGQFVLAGMNVYPSEFENIKEEYSVRGYPTICYFEKGRFLFQYD-SYGSTAEDIVEWLKNPQPPQPQVPETPWADEGG--SVYHLSDEDFDQFVKEHSS-VLVMFHAPWCGHCKKMKPEFESAAEVLHGEGDSSGVLAAVDATVNKALAERFHIAEFPTLKYFK-NGEKYAVPALRTKKSFIEWM 381
BLAST of Protein disulfide-isomerase A5 vs. UniProt/SwissProt
Match: sp|Q8NBS9|TXND5_HUMAN (Thioredoxin domain-containing protein 5 OS=Homo sapiens OX=9606 GN=TXNDC5 PE=1 SV=2) HSP 1 Score: 154.451 bits (389), Expect = 7.098e-40 Identity = 96/358 (26.82%), Postives = 167/358 (46.65%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQF---AAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFV-----HNPTQPPPQKDVPWSEETNEIDF-LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSN-------------SEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
MF+APWCG+C++++P + +++ +++ D + D+ A + GYP++ L + G + KY+G + L ++ P P P+ + P + E + + L+ +NF ++ Q + I F+APWCGHCKA P + A + ++ V VDCT ++C+ + G+P +F GK E L+R E +T P L A + ++ L +NNF I + + FY+PWCGHC + P++ + K L IA VD T E ++ ++Y V G+PTL+ F ++ G R D++ F++ + D
Sbjct: 83 MFFAPWCGHCQRLQPTWNDLGDKYNSMEDAKVYVA-KVDCTAHSDVCSAQGVRGYPTLKLFKPGQEAVKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQGDHFI-KFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCTQHY--ELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYVESQLQRTETGATETVTPSEAPVLAAEPEADK----GTVLALTENNFDDTIAEGIT--FIKFYAPWCGHCKTLAPTWEELSKKEFPGLAGVKIAEVDCTAERNICSKYSVRGYPTLLLFRGGKKVSEHSGGRDLDSLHRFVLSQAKD 430 HSP 2 Score: 102.449 bits (254), Expect = 6.114e-22 Identity = 63/233 (27.04%), Postives = 111/233 (47.64%), Query Frame = 1
Query: 310 LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM--AENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPG----HEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
L A+ + + Q + VMF+APWCGHC+ +P + ++ E+ KV VDCT S D+C+++G+ G+P + F G+ +Y R+ +M N + P + + L +NF ++ + F++PWCGHC A+ P++ A L+ + I VD T L + +V G+PTL++F +Y G+R +++ E++
Sbjct: 65 LYTADMFTHGIQSAAHFVMFFAPWCGHCQRLQPTWNDLGDKYNSMEDAKVYVAKVDCTAHS--DVCSAQGVRGYPTLKLFKPGQEA--VKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQ--GDHFIKFFAPWCGHCKALAPTWEQLALGLEH-SETVKIGKVDCTQHYELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYV 290
BLAST of Protein disulfide-isomerase A5 vs. TrEMBL
Match: R7UHJ2 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_224024 PE=4 SV=1) HSP 1 Score: 263.462 bits (672), Expect = 8.077e-75 Identity = 141/344 (40.99%), Postives = 200/344 (58.14%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTD-----DLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVP--WSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP-------QLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWCG+CK MKP++ AA +K G+ G L+ D +L + + G+P+V +DG + FK + + + +++F+ +P +PPP WSE +E++ LTD NF + + + +VMFYAPWCGHCKATKP+FTSAA+ + KVAF VDCT D+CT ++G+P FRYF+YGK+ + +Y+ R+E DF+AFM +P NP + W PGHE + L NF Q +S LVMFY+PWCGHC +MKP++ AA L+ +AAVDAT LA++Y+V G+PTL YF + Y +R FM
Sbjct: 297 MFYAPWCGHCKNMKPEYVQAAAAMKED----GVEGALAAVDATKAQELAGKYGVKGFPTVIYFKDGEEAFKV-NERTADKIVEFMKDPKEPPPPPPPEPEWSEVESEVNHLTDENFRSFTKKKKHTLVMFYAPWCGHCKATKPEFTSAADSFKDESKVAFAAVDCTKTK--DLCTKYDVSGYPTFRYFSYGKD--DFKYTGGRKEPDFIAFMKDPQNPPKVSPPPAANPLDMWADAPGHENVHHLTTANFAQ-FLSENPSTLVMFYAPWCGHCKSMKPAYAEAAQLLKENNKPGALAAVDATAHPDLASRYEVKGYPTLKYFKDGAFVMDYSKQRNTKEFVTFM 630 HSP 2 Score: 225.328 bits (573), Expect = 8.638e-61 Identity = 129/334 (38.62%), Postives = 178/334 (53.29%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNP-TQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENK-KVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTN--PANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWCG+CK++KP +AAAAT LKG+++ +GIN D + LK FN++GYP++ I+ G KY G N + ++ ++ +P P+K+ WS+E +++ L D F E+L + SV+VMFYAPWCGHCK KP++ AA M E+ + A VD T ++ G+ GFP YF G+ ER D V FM + P W + + L NF ++ LVMFY+PWCGHC A KP F +AA+ + AAVD T L +Y VSG+PT YFS D KY G R + FM
Sbjct: 175 MFYAPWCGHCKRLKPDYAAAATELKGQAVLVGINADKPEFNPLKVDFNVSGYPTLHYIEKGKPKMKYGGKNDQNGIVSWMKDPQEPKEPEKEAEWSDEESDVHHLLDDTFDEFLTANPSVLVMFYAPWCGHCKNMKPEYVQAAAAMKEDGVEGALAAVDATKAQ--ELAGKYGVKGFPTVIYFKDGEEAFKVN---ERTADKIVEFMKDPKEPPPPPPPEPEWSEV--ESEVNHLTDENF-RSFTKKKKHTLVMFYAPWCGHCKATKPEFTSAADSFKD-ESKVAFAAVDCTKTKDLCTKYDVSGYPTFRYFSYGKDDFKYTGGRKEPDFIAFM 499 HSP 3 Score: 201.83 bits (512), Expect = 2.598e-52 Identity = 117/340 (34.41%), Postives = 175/340 (51.47%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVP-------WSEETNE--IDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKV-AFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWCG+CK KP+F +AA + K +S D + T DL ++++GYP+ G FKY G + I F+ +P PP P W++ + LT ANF ++L ++ S +VMFYAPWCGHCK+ KP + AA + EN K A VD T + D+ + + G+P +YF G + YS +R +FV FM NP P+L+ + E + +N +Q+ ++ ++ LVMFY+PWCGHC A KP+F AA +L IAAV+ + K+SG+P+ Y++ Y G RT + ++
Sbjct: 420 MFYAPWCGHCKATKPEFTSAADSFKDESKVAFAAVDCTKTKDLCTKYDVSGYPTFRYFSYGKDDFKYTGGRKEPDFIAFMKDPQNPPKVSPPPAANPLDMWADAPGHENVHHLTTANFAQFLSENPSTLVMFYAPWCGHCKSMKPAYAEAAQLLKENNKPGALAAVDAT--AHPDLASRYEVKGYPTLKYFKDGAFVMD--YSKQRNTKEFVTFMENPG-PELEWSDEQNEV---EHLT----SNTMQSFLTSSADVLVMFYAPWCGHCKAAKPAFTEAA-ELLIDESDKHIAAVNCIANKAACEEAKISGYPSFKYYNRGIYVADYNGGRTAEDFANYL 746 HSP 4 Score: 150.214 bits (378), Expect = 1.821e-34 Identity = 79/218 (36.24%), Postives = 115/218 (52.75%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARR 651
MFYAPWCG+CK MKP +A AA LK + + D + DL + + GYP++ +DG V Y + + + F+ NP ++ WS+E NE++ LT +L V+VMFYAPWCGHCKA KP FT AA + + V+C A C I+G+P F+Y+N G ++ Y+ R +DF ++ +P PQLD +
Sbjct: 550 MFYAPWCGHCKSMKPAYAEAAQLLKENNKPGALAAVDATAHPDLASRYEVKGYPTLKYFKDGAFVMDYSKQRNTKEFVTFMENPGP-----ELEWSDEQNEVEHLTSNTMQSFLTSSADVLVMFYAPWCGHCKAAKPAFTEAAELLIDESDKHIAAVNCIANKAA--CEEAKISGYPSFKYYNRGIYVAD--YNGGRTAEDFANYLKSP--PQLDKKE 756 HSP 5 Score: 146.362 bits (368), Expect = 3.673e-33 Identity = 88/277 (31.77%), Postives = 140/277 (50.54%), Query Frame = 1
Query: 175 QDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDF--LTDANFYEYLDQHES--VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNP-ANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+DG YE + E++ F+ +PT D+PW+E++ D + Y L + E ++VMFYAPWCGHCK KP + +AA ++ + +G++ F + ++G+P Y GK +Y + +++ V++M +P + + W E+ V ++ ++ LVMFY+PWCGHC MKP +V AA ++ +AAVDAT LA +Y V GFPT++YF +G+ + ERT D I EFM
Sbjct: 113 KDGEFNTDYERKYTVESMSNFLRDPTG-----DIPWNEDSTATDVVHIETMKAYSSLMKKEKRPMLVMFYAPWCGHCKRLKPDYAAAATEL--KGQAVLVGINADKPEFNPLKVDFNVSGYPTLHYIEKGK--PKMKYGGKNDQNGIVSWMKDPQEPKEPEKEAEWS---DEESDVHHLLDDTFDEFLTANPSVLVMFYAPWCGHCKNMKPEYVQAAAAMKEDGVEGALAAVDATKAQELAGKYGVKGFPTVIYFK-DGEEAFKVNERTADKIVEFM 376
BLAST of Protein disulfide-isomerase A5 vs. TrEMBL
Match: A0A1X7VMG8 (Uncharacterized protein OS=Amphimedon queenslandica OX=400682 GN=100634383 PE=4 SV=1) HSP 1 Score: 246.899 bits (629), Expect = 6.524e-71 Identity = 133/350 (38.00%), Postives = 192/350 (54.86%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVP------WSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM-AENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM--IKKHSDIIRHE 1023
MFYAPWCG+C+ +KP+FAAAAT+LKGK + G+N D T ++ +N+TG+P++ ++G + Y G +++IK++ +P PP ++V WS E N + LT NF +D S +V FYAPWCGHCKA KP + AA + +EN VD T E ++ + ++GFP +YF+ GK + Y R + FV FM NP P + + W I E V + + + T ALVMFY+PWCGHC A KP F++AA L+ + L AAVD T + Q V G+PT++Y S KYMG R + EF+ K+ I++ E
Sbjct: 171 MFYAPWCGHCQLLKPEFAAAATSLKGKQVLAGMNLDKPETMLTREEYNVTGFPTLLYFEEGKVKYPYSGGRDTDSIIKWLSDPQPPPSTQEVEQEAGANWSTELNNVVHLTSENFQSVIDSSPSTLVTFYAPWCGHCKAMKPDYNEAAKLLESENILGTLAAVDATAER--ELASHYQVSGFPTIKYFSNGKELYD--YGYPRTTESFVEFMKNP-QPPPEKEKDWSEI---ETGVHHLTDETYKPFIKKTKHALVMFYAPWCGHCKAAKPEFIDAAASLKEDKKTSL-AAVDCTKYAQICDQNDVQGYPTILYMSYGKKSFKYMGPRDSNGFVEFLRNPNKYVSIVKDE 511 HSP 2 Score: 161.384 bits (407), Expect = 2.303e-39 Identity = 98/285 (34.39%), Postives = 151/285 (52.98%), Query Frame = 1
Query: 172 IQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFL---TDANFYEYL-DQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSE--GITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP------QLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+DG+ Y+ K +L+ F+ +PT D PWSE+ D + Y++L + + +++MFYAPWCGHC+ KP+F +AA + + +A + +D + + T E +TGFP YF GK YS R+ D + ++++P P + +A +W + +V L NF Q+++ + LV FY+PWCGHC AMKP + AA L++ +AAVDAT E LA+ Y+VSGFPT+ YFS + Y RT ++ EFM
Sbjct: 108 FKDGSYHKDYDRLLRKSSLLDFMKDPTS-----DAPWSEDPTSKDVRHIESSTGLYKFLAKEKKPILLMFYAPWCGHCQLLKPEFAAAATSLKGKQVLAGMNLDKPE----TMLTREEYNVTGFPTLLYFEEGK--VKYPYSGGRDTDSIIKWLSDPQPPPSTQEVEQEAGANWSTELNN--VVHLTSENF-QSVIDSSPSTLVTFYAPWCGHCKAMKPDYNEAAKLLESENILGTLAAVDATAERELASHYQVSGFPTIKYFSNGKELYDYGYPRTTESFVEFM 378
BLAST of Protein disulfide-isomerase A5 vs. TrEMBL
Match: A0A1S3HT50 (protein disulfide-isomerase A5-like OS=Lingula unguis OX=7574 GN=LOC106157954 PE=4 SV=1) HSP 1 Score: 249.98 bits (637), Expect = 1.972e-69 Identity = 134/347 (38.62%), Postives = 198/347 (57.06%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQ-----AFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQK--DVPWSEETN-EIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQ---------LDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWCG+CK+MKP++ AA LK + G+ G L+ D K+ + G+P+V +DG F + + + + L++F+ +P +PPP + WSE+ + ++FLT+ F ++ + + +VMFYAPWCGHCK KP +T+AA ++ K+A+ VDCT + +CT+ ++G+P F+Y+NYGKN +Y REE DFV FM +P NP+ W + G E + L F + V LVMFY+PWCGHC AMKP++ AA KL+ + ++AAVDAT E L +YKV+GFPTL YF +Y R+ + FM
Sbjct: 294 MFYAPWCGHCKKMKPEYVQAAAQLKEE----GVEGILAAVDATKERKAAETLKVQGFPTVKYFKDGEYAFDF-NERTADKLVEFMKDPKEPPPPPPPEAEWSEQEDIHVNFLTEETFKPFMKKKKHTLVMFYAPWCGHCKKAKPHYTNAAEIYKDDPKIAYAAVDCT--KYQSLCTANDVSGYPTFKYYNYGKNPQ--QYQGGREEADFVNFMKDPNNPEGSTPPPPPQQTPEEQWADVAGAENLNHLTSGTF-EAFVKENPSVLVMFYAPWCGHCKAMKPAYGEAAQKLKEQNINGVLAAVDATQERDLTNKYKVTGFPTLKYFRNGEVAFEYNRGRSTSDLVSFM 630 HSP 2 Score: 223.402 bits (568), Expect = 8.586e-60 Identity = 126/335 (37.61%), Postives = 177/335 (52.84%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHN-PTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFI-GVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSL---EREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWCG+CK++KP +AAAAT LKG S+ +G++ D ++Q +NITG+P++ ++G K + Y G N+KE +I ++ N P+K+ W++E E+ L D+NF EY+ H SV+VMFYAPWCGHCK KP++ AA Q+ E + VD T E A + + GFP +YF G EY+ ER D V FM +P P + V + + LVMFY+PWCGHC KP + NAA ++ P AAVD T L VSG+PT Y++ + +Y G R + FM
Sbjct: 172 MFYAPWCGFCKRLKPDYAAAATELKGHSVLVGMDVDKPEMMPVRQEYNITGFPTIVYFENGKKKYNYGGQNNKEGIISWMKNPEPPKEPEKEAEWADEETEVVHLQDSNFDEYVKDHPSVLVMFYAPWCGHCKKMKPEYVQAAAQLKEEGVEGILAAVDATKERKA--AETLKVQGFPTVKYFKDG------EYAFDFNERTADKLVEFMKDPKEPPPPPPPEAEWSEQEDIHVNFLTEETFKPFMKKKKHTLVMFYAPWCGHCKKAKPHYTNAA-EIYKDDPKIAYAAVDCTKYQSLCTANDVSGYPTFKYYNYGKNPQQYQGGREEADFVNFM 497 HSP 3 Score: 191.43 bits (485), Expect = 1.726e-48 Identity = 123/349 (35.24%), Postives = 172/349 (49.28%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQK--------------DVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAE-NKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARR---HWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWV-KYMGERTDDAITEFM 990
MFYAPWCG+CK+ KP + AA K D + L A +++GYP+ G +Y+G + + F+ +P P DV +E N LT F ++ ++ SV+VMFYAPWCGHCKA KP + AA ++ E N VD T E D+ +TGFP +YF G+ EY+ R D V+FMTNP + + + W P + I L + F +I S LVMFY+PWCGHC KP F AAN LQ + +AAVD T + L ++ V+G+PT Y+S G++V KY G R+ T+F+
Sbjct: 418 MFYAPWCGHCKKAKPHYTNAAEIYKDDPKIAYAAVDCTKYQSLCTANDVSGYPTFKYYNYGKNPQQYQGGREEADFVNFMKDPNNPEGSTPPPPPQQTPEEQWADVAGAENLNH---LTSGTFEAFVKENPSVLVMFYAPWCGHCKAMKPAYGEAAQKLKEQNINGVLAAVDATQER--DLTNKYKVTGFPTLKYFRNGEVAF--EYNRGRSTSDLVSFMTNPEEKKPEPPKPEAQWSDTPSN--IRHLTQATFTPSI-SDMEQVLVMFYAPWCGHCKRAKPEFEEAANSLQGST-TQFLAAVDCTAASELCSKEGVTGYPTFRYYS-RGEFVKKYQGGRSKQDFTDFL 754 HSP 4 Score: 155.992 bits (393), Expect = 3.023e-36 Identity = 105/323 (32.51%), Postives = 163/323 (50.46%), Query Frame = 1
Query: 40 KPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYP-SVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETN--EIDFLTDANFYEYL--DQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARR-HWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
K + A +KGK+ I + D S L + F I+ P + +DG Y+ ++++ F+ +PT D+PW E+++ ++ L + L + + ++VMFYAPWCG CK KP + +AA ++ + +G+D + ITGFP YF GK N Y + ++ +++M NP P+ + W +V L+ +NF + + H S LVMFY+PWCGHC MKP +V AA +L+ ++AAVDAT E A KV GFPT+ YF +G++ ERT D + EFM
Sbjct: 66 KSLLSEVALEMKGKATVITV--DCSEAKKLCKKFKISPSPVELKHYKDGEFNKDYDRKEVVKSMVSFLQDPTG-----DLPWVEDSSAEDVTHLENPGALNKLLSKEKKPILVMFYAPWCGFCKRLKPDYAAAATELKGHS--VLVGMDVDKPEMMPVRQEYNITGFPTIVYFENGKKKYN--YGGQNNKEGIISWMKNPEPPKEPEKEAEW--ADEETEVVHLQDSNFDEYVKDHPS-VLVMFYAPWCGHCKKMKPEYVQAAAQLKEEGVEGILAAVDATKERKAAETLKVQGFPTVKYFK-DGEYAFDFNERTADKLVEFM 373 HSP 5 Score: 153.295 bits (386), Expect = 1.890e-35 Identity = 85/223 (38.12%), Postives = 122/223 (54.71%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTD-----DLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKD---VPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDA 645
MFYAPWCG+CK MKP + AA LK ++I NG L+ D DL + +TG+P++ ++G F+Y S L+ F+ NP + P+ WS+ + I LT A F + E V+VMFYAPWCGHCK KP+F AAN + + VDCT S ++C+ EG+TG+P FRY++ G+ +Y R + DF F+ + + DA
Sbjct: 550 MFYAPWCGHCKAMKPAYGEAAQKLKEQNI----NGVLAAVDATQERDLTNKYKVTGFPTLKYFRNGEVAFEYNRGRSTSDLVSFMTNPEEKKPEPPKPEAQWSDTPSNIRHLTQATFTPSISDMEQVLVMFYAPWCGHCKRAKPEFEEAANSLQGSTTQFLAAVDCTAAS--ELCSKEGVTGYPTFRYYSRGEFV--KKYQGGRSKQDFTDFLNSEKSTASDA 764
BLAST of Protein disulfide-isomerase A5 vs. TrEMBL
Match: A0A2T7PR44 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_02829 PE=4 SV=1) HSP 1 Score: 248.44 bits (633), Expect = 2.475e-69 Identity = 131/336 (38.99%), Postives = 194/336 (57.74%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQP-PPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQM-AENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSL---EREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFS-PNGDWVKYMGERTDDAITEFM 990
MFYAPWCGYCK++KP+FAAAAT +KGK+ +GI+ D ++ +N+TG+P+++ + G +F+Y G+N+K+ +I +++NP P P+K+ WSEE +E+ L D+NF Y+ +H SV+VMFYAPWCGHCK KP +T AA + +E VD T + I GFP +YF G EY+ ER++D + F+ NP P ++V L + NF + + +LVMFY+PWCGHC KP F++AA + + L AVD TT+ + + V+G+PT YF+ N + KYMG R ++ FM
Sbjct: 161 MFYAPWCGYCKRLKPEFAAAATEVKGKAQLVGIDVDKPQMMGMRTQYNVTGFPTLYYFEKGRAMFRYGGENNKDGIITWLNNPRPPEEPKKEAEWSEEPSEVVHLEDSNFDTYIKEHSSVLVMFYAPWCGHCKKMKPDYTEAAETIKSEGLTSVLAAVDATKNR--KLAEDYKIKGFPTLKYFKDG------EYAFEVNERDKDKIIEFIKNPQEPPPPPAPEPSWEEIESSVVHLNEENF-KFTLKKRKHSLVMFYAPWCGHCKKAKPEFMSAAAQFKD-DSKVLFGAVDCTTQQSICTAHDVTGYPTFKYFNYLNKNSQKYMGGREENDFVNFM 486 HSP 2 Score: 237.654 bits (605), Expect = 2.472e-65 Identity = 136/345 (39.42%), Postives = 193/345 (55.94%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNE--IDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFM-------------TNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEF 987
MFYAPWCG+CK+MKP + AA T+K + + + D + L + + I G+P++ +DG F+ + K+ +I+F+ NP +PPP S E E + L + NF L + + +VMFYAPWCGHCK KP+F SAA Q ++ KV F VDCT + ICT+ +TG+P F+YFNY N ++ +Y REE+DFV FM T P PQ + W I G + I L F + ++ LVMFY+PWCGHC MKP++++AA +L T +P +AAVDAT LA Y + GFPTL YF + Y G R+++ + F
Sbjct: 283 MFYAPWCGHCKKMKPDYTEAAETIKSEGLTSVLAAVDATKNRKLAEDYKIKGFPTLKYFKDGEYAFEV-NERDKDKIIEFIKNPQEPPPPPAPEPSWEEIESSVVHLNEENFKFTLKKRKHSLVMFYAPWCGHCKKAKPEFMSAAAQFKDDSKVLFGAVDCTTQQ--SICTAHDVTGYPTFKYFNY-LNKNSQKYMGGREENDFVNFMRDPLSPSPTLPPATQP-QPQTNPGEDWADIEGQKNINFLTAETF-DSFIAEQPSVLVMFYAPWCGHCKKMKPAYMSAATRLMTEVPDAKLAAVDATKYRELANTYSIKGFPTLKYFEGGKEISDYGGGRSEEDLVNF 621 HSP 3 Score: 174.096 bits (440), Expect = 1.469e-42 Identity = 106/322 (32.92%), Postives = 165/322 (51.24%), Query Frame = 1
Query: 49 FAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQ---DGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFL---TDANFYEYLDQHESV-IVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARR-HWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
F A +KGK+ +N DD K + P F ++ DG Y+ + ++++ F+ +PT D+PW E+ +D + ++ + L + ++ +VMFYAPWCG+CK KP+F +AA ++ K +G+D + T +TGFP YF G+ + Y E +D + ++ NP P+ + W P +V LE +NF I H+S LVMFY+PWCGHC MKP + AA +++ + ++AAVDAT LA YK+ GFPTL YF +G++ + ER D I EF+
Sbjct: 58 FEQVAEEMKGKATIAFVN----CGDDKKFCKKLKINPDTFELKHYKDGEFNKDYDRKLAAKSMVNFLMDPTG-----DIPWDEDPTAVDVIHIDSEDGLNKLLKKDKAATLVMFYAPWCGYCKRLKPEFAAAATEV--KGKAQLVGIDVDKPQMMGMRTQYNVTGFPTLYYFEKGR--AMFRYGGENNKDGIITWLNNPRPPEEPKKEAEWSEEPSE--VVHLEDSNFDTYIKEHSS-VLVMFYAPWCGHCKKMKPDYTEAAETIKSEGLTSVLAAVDATKNRKLAEDYKIKGFPTLKYFK-DGEYAFEVNERDKDKIIEFI 362 HSP 4 Score: 159.458 bits (402), Expect = 1.524e-37 Identity = 115/361 (31.86%), Postives = 165/361 (45.71%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNK-VFKYEGDNSKEALIKFVHN----------PTQPPPQKDV--PWS--EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQ-MAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFM--TNPANPQ--LDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHE 1023
MFYAPWCG+CK+ KP+F +AA K S + D +T + A ++TGYP+ NK KY G + + F+ + TQP PQ + W+ E I+FLT F ++ + SV+VMFYAPWCGHCK KP + SAA + M E VD T + ++ + I GFP +YF GK S+ Y R E+D V F + P P+ L + H + +P L NF + + + ALV FY+ C C P F A L L A V+ T + L + FPT V + +Y +T DA + +K + + + E
Sbjct: 406 MFYAPWCGHCKKAKPEFMSAAAQFKDDSKVLFGAVDCTTQQSICTAHDVTGYPTFKYFNYLNKNSQKYMGGREENDFVNFMRDPLSPSPTLPPATQPQPQTNPGEDWADIEGQKNINFLTAETFDSFIAEQPSVLVMFYAPWCGHCKKMKPAYMSAATRLMTEVPDAKLAAVDAT--KYRELANTYSIKGFPTLKYFEGGKEISD--YGGGRSEEDLVNFFKGSKPPTPEESLPSFFHSKWVPS------LSGANF-SSTLRNREAALVFFYADSCPKCTKYWPEFAAAGEALVNQL-----ATVNCDTYSDLCGLEGIRKFPTFVLYMQGQRKDEYRDAKTKDAFLNY-VKTQTPLTKEE 749
BLAST of Protein disulfide-isomerase A5 vs. TrEMBL
Match: A0A423TN25 (Protein disulfide-isomerase A5 OS=Penaeus vannamei OX=6689 GN=C7M84_003451 PE=4 SV=1) HSP 1 Score: 243.817 bits (621), Expect = 1.441e-68 Identity = 140/344 (40.70%), Postives = 195/344 (56.69%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQA-----FNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVP--WSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPAN-------PQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWCG+CK+MKP++ AAAT LK K GI G L+ D K+ F++ GYP++ +DG F K+ +I+F+ +P +PPP WSE + + LTD NF +L + + V+VMFYAPWCGHCK KP+FT+AA++ ++ KV F VDCT + +C ++G+P F+YFNY KNT Y+ R E DFV FM +P N P + W + G E + L + F I ALVMFY+PWCGHC AMK + AA +L + ++A VDAT E LA+++++ GFPTL +F +Y R I +FM
Sbjct: 283 MFYAPWCGHCKRMKPEYTAAATVLKEK----GIPGKLAAVDATKETKLGAEFSVKGYPTIKYFKDGEYAFDVSSAREKDKIIEFMSDPKEPPPPPPPEKPWSEVESSVIHLTDENFKPFLKKKKHVLVMFYAPWCGHCKKAKPEFTAAADKFVDDPKVEFAAVDCT--AHQTLCGVNDVSGYPTFKYFNYYKNT--KPYNGGRTEIDFVKFMEDPENPLSGSPPPPPSPQEEWASVEGSELLHHLTEATFDAFIKDRE--ALVMFYAPWCGHCKAMKGDYGQAAVELAAEGQNGVLATVDATVERSLASRFQIRGFPTLKFFRRGQLVTEYNRSRKAADIIDFM 616 HSP 2 Score: 216.083 bits (549), Expect = 2.283e-58 Identity = 120/333 (36.04%), Postives = 179/333 (53.75%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPP--PQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVA-FIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWCG+CK++KP ++AAA LK + ++ + + +NITG+P++ + G + YEG+N+K ++ F+ NP + P P+++V WSE +++ LTDA F+E++ SV+VMFYAPWCGHCK KP++T+AA + E VD T E+ + + G+P +YF G+ + S RE+D + FM++P P +++ L NF + + LVMFY+PWCGHC KP F AA+K P AAVD T L VSG+PT YF+ + Y G RT+ +FM
Sbjct: 161 MFYAPWCGFCKRLKPDYSAAAAELKDTHTLVAMDVNKPENSAARIKYNITGFPTLVYFEAGAPKYTYEGENNKAGIVSFMKNPGKAPEKPKEEV-WSETPSDVSHLTDATFHEFIKGEASVLVMFYAPWCGHCKRMKPEYTAAATVLKEKGIPGKLAAVDATKET--KLGAEFSVKGYPTIKYFKDGEYAFD--VSSAREKDKIIEFMSDPKEPPPPPPPEKPWSEVESSVIHLTDENF-KPFLKKKKHVLVMFYAPWCGHCKKAKPEFTAAADKFVD-DPKVEFAAVDCTAHQTLCGVNDVSGYPTFKYFNYYKNTKPYNGGRTEIDFVKFM 486 HSP 3 Score: 147.902 bits (372), Expect = 4.849e-34 Identity = 94/285 (32.98%), Postives = 140/285 (49.12%), Query Frame = 1
Query: 157 PSVFLIQ---DGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDF--LTDAN-FYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPAN-PQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
PS ++++ DG+ Y+ + +++ F+ +P+ D+PW E+ +D + D N + L + ++ ++MFYAPWCG CK KP +++AA ++ + + + V+ + S A I ITGFP YF G Y E + V+FM NP P+ W P + L F + I S LVMFY+PWCGHC MKP + AA L+ +AAVDAT ET L A++ V G+PT+ YF R D I EFM
Sbjct: 91 PSTYVLKHYKDGDFHKDYDRRETVQSMSTFLRDPSG-----DIPWDEDEQGVDVVHINDMNALNKLLKKEKAFMMMFYAPWCGFCKRLKPDYSAAAAELKDTHTLVAMDVNKPENSAARI--KYNITGFPTLVYFEAG--APKYTYEGENNKAGIVSFMKNPGKAPEKPKEEVWSETPSD--VSHLTDATFHEFIKGEAS-VLVMFYAPWCGHCKRMKPEYTAAATVLKEKGIPGKLAAVDATKETKLGAEFSVKGYPTIKYFKDGEYAFDVSSAREKDKIIEFM 363
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Cavefish
Match: pdia5 (protein disulfide isomerase family A member 5 [Source:NCBI gene;Acc:103026706]) HSP 1 Score: 196.823 bits (499), Expect = 2.189e-55 Identity = 123/339 (36.28%), Postives = 174/339 (51.33%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYE--GDNSKEALIKFVHNPTQPPPQK-DVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSE--GITGFPIFRYFNYGKNTSNSEYSLE--REEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATT-ETH-LAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWCG CK+M+P F AT KGK + G+N + D LKQ FN+ GYP+ + G + YE G +K+ + ++ NP P P+ +VPWSE + + LTD +F +L++H S ++MFYAPWCGHCK KP++ AA + NK GV ++ E I+GFP +YF +Y+L R +D + ++ NP P + W P ++ L +F + + ALVMFY+PWC HC P F+ AA + AAVD T + H L Q V G+PT Y++ KY GER + T FM
Sbjct: 185 MFYAPWCGVCKRMQPIFQQTATETKGKYVLAGMNVHPAEFDGLKQEFNVKGYPTFCYFEKGKFLHHYENYGATAKD-IADWLKNPQPPQPKTPEVPWSESDSAVFHLTDESFDGFLEEHPSALIMFYAPWCGHCKKMKPEYDEAAEVL--NKDADTPGVLAAVDTTIHKTVGERFKISGFPTVKYF----EKREEKYTLPHLRTKDKIIEWLQNPQAPP-PPEQSWEEKPS--SVSHLGAEDF-REALKKKKHALVMFYAPWCPHCKNAVPHFITAAELFKDDR-KIAYAAVDCTKGQNHELCKQEGVEGYPTFNYYNYGKFSEKYNGERGEAGFTGFM 511 HSP 2 Score: 126.716 bits (317), Expect = 1.224e-30 Identity = 94/320 (29.38%), Postives = 150/320 (46.88%), Query Frame = 1
Query: 61 ATTLKGKSIFIGINGDLSTTDDL--KQAFNITGYPSVFLI---QDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHES-VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHE---AIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPS-CLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
A +KG+ +N S L K + P+ F I +DG +Y + ++++ F+ +PT P ++ P +++ I+ T+ +F + L + E +++MFYAPWCG CK +P F A + K G++ F + + G+P F YF GK + E + D ++ NP PQ +P E A+ L +F + H S AL+MFY+PWCGHC MKP + AA L + ++AAVD T + ++K+SGFPT+ YF + RT D I E++
Sbjct: 80 AQAVKGQGTIAWVNCGDSEGRKLCKKVKVDPGSKPAGFEILHYKDGTFHTEYSRPATLKSMVAFLKDPTGAPLWEENPEAKDVVHIE--TEKDFRKLLKREERPILMMFYAPWCGVCKRMQPIFQQTATET--KGKYVLAGMNVHPAEFDGLKQEFNVKGYPTFCYFEKGKFLHHYE-NYGATAKDIADWLKNPQPPQPKT----PEVPWSESDSAVFHLTDESFDGFLEEHPS-ALIMFYAPWCGHCKKMKPEYDEAAEVLNKDADTPGVLAAVDTTIHKTVGERFKISGFPTVKYFEKREEKYTLPHLRTKDKIIEWL 389
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Cavefish
Match: pdia5 (protein disulfide isomerase family A member 5 [Source:NCBI gene;Acc:103026706]) HSP 1 Score: 186.422 bits (472), Expect = 1.298e-51 Identity = 119/339 (35.10%), Postives = 172/339 (50.74%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYE--GDNSKEALIKFVHNPTQPPPQK-DVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSE--GITGFPIFRYFNYGKNTSNSEYSLE--REEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATT-ETH-LAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+ +A WCG CK+M+P F AT KGK + G+N + D LKQ FN+ GYP+ + G + YE G +K+ + ++ NP P P+ +VPWSE + + LTD +F +L++H S ++MFYAPWCGHCK KP++ AA + NK GV ++ E I+GFP +YF +Y+L R +D + ++ NP P + W P ++ L +F + + ALVMFY+PWC HC P F+ AA + AAVD T + H L Q V G+PT Y++ KY GER + T FM
Sbjct: 180 LVFAWWCGVCKRMQPIFQQTATETKGKYVLAGMNVHPAEFDGLKQEFNVKGYPTFCYFEKGKFLHHYENYGATAKD-IADWLKNPQPPQPKTPEVPWSESDSAVFHLTDESFDGFLEEHPSALIMFYAPWCGHCKKMKPEYDEAAEVL--NKDADTPGVLAAVDTTIHKTVGERFKISGFPTVKYF----EKREEKYTLPHLRTKDKIIEWLQNPQAPP-PPEQSWEEKPS--SVSHLGAEDF-REALKKKKHALVMFYAPWCPHCKNAVPHFITAAELFKDDR-KIAYAAVDCTKGQNHELCKQEGVEGYPTFNYYNYGKFSEKYNGERGEAGFTGFM 506 HSP 2 Score: 107.842 bits (268), Expect = 2.169e-24 Identity = 87/319 (27.27%), Postives = 140/319 (43.89%), Query Frame = 1
Query: 61 ATTLKGKSIFIGINGDLSTTDDL--KQAFNITGYPSVFLI---QDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHE---AIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPS-CLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
A +KG+ +N S L K + P+ F I +DG +Y + ++++ F+ +PT P ++ P +++ I+ + + E + +A WCG CK +P F A + K G++ F + + G+P F YF GK + E + D ++ NP PQ +P E A+ L +F + H S AL+MFY+PWCGHC MKP + AA L + ++AAVD T + ++K+SGFPT+ YF + RT D I E++
Sbjct: 74 AQAVKGQGTIAWVNCGDSEGRKLCKKVKVDPGSKPAGFEILHYKDGTFHTEYSRPATLKSMVAFLKDPTGAPLWEENPEAKDVVHIETEKVSISFRRRLWTEITFPLVFAWWCGVCKRMQPIFQQTATET--KGKYVLAGMNVHPAEFDGLKQEFNVKGYPTFCYFEKGKFLHHYE-NYGATAKDIADWLKNPQPPQPKT----PEVPWSESDSAVFHLTDESFDGFLEEHPS-ALIMFYAPWCGHCKKMKPEYDEAAEVLNKDADTPGVLAAVDTTIHKTVGERFKISGFPTVKYFEKREEKYTLPHLRTKDKIIEWL 384
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Cavefish
Match: pdia4 (protein disulfide isomerase family A member 4 [Source:NCBI gene;Acc:103026638]) HSP 1 Score: 144.821 bits (364), Expect = 2.502e-36 Identity = 87/261 (33.33%), Postives = 134/261 (51.34%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAE-NKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKKKAD 1065
+E N + LTDANF +++ ++V+V FYAPWCGHCK P++ A + E + + VD T + + + ++G+P + G+ EY +R E V + A P W+ P EA ++L K+NF ++V++ LV FY+PWCGHC + P + AA +L P L+A VDAT E LA+++ V+G+PTL F G Y G R I ++M ++ + +A K QE K D
Sbjct: 58 KEENGVLVLTDANFDTFMEGKDTVLVEFYAPWCGHCKQFAPEYEKIAQVLKESDPPIPVAKVDATKAT--ALGSRFEVSGYPTIKILKKGEPV---EYDGDRSEKAIVERVKEVAQPD------WK--PPPEATLVLTKDNF-DDVVNNADIILVEFYAPWCGHCKRLAPEYEKAAKELSNRTPPILLAKVDATVENDLASRFDVTGYPTLKMFR-KGKVFDYNGPREKIGIVDYMSEQAGPPSKQVQALKQIQELLKDGD 303 HSP 2 Score: 122.094 bits (305), Expect = 1.001e-28 Identity = 72/236 (30.51%), Postives = 122/236 (51.69%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMA-ENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP---QLDARRHWRRI--PGHEAIVI 690
FYAPWCG+CKQ P++ A LK I + D + L F ++GYP++ +++ G V +Y+GD S++A+++ V QP W LT NF + ++ + ++V FYAPWCGHCK P++ AA +++ + VD T E+ D+ + +TG+P + F GK +Y+ RE+ V +M+ A P Q+ A + + + G +A+++
Sbjct: 85 FYAPWCGHCKQFAPEYEKIAQVLKESDPPIPVAKVDATKATALGSRFEVSGYPTIKILKKGEPV-EYDGDRSEKAIVERVKEVAQPD------WKPPPEATLVLTKDNFDDVVNNADIILVEFYAPWCGHCKRLAPEYEKAAKELSNRTPPILLAKVDATVEN--DLASRFDVTGYPTLKMFRKGKVF---DYNGPREKIGIVDYMSEQAGPPSKQVQALKQIQELLKDGDDALIV 308 HSP 3 Score: 64.6994 bits (156), Expect = 1.578e-10 Identity = 46/160 (28.75%), Postives = 82/160 (51.25%), Query Frame = 1
Query: 562 SNSEYSLEREEDD-------FVAFMTNPANPQLDARRHWRRIPGHEAIV-ILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYM-GERTDDAITEFMIKKHS 1005
+Y++E EE D VAF P + ++ +P ++ V ++ F + ++ L+ FY+PWCGHC ++P ++ A K + + +IA +DAT YKV GFPT +YF+P+ + +K+ GER + ++F I++H+
Sbjct: 481 GGKKYAMEPEEFDTEVLREFVVAFKKGKLKPIIKSQP----VPKNKGPVKVIVGKTFDEIVLDKKKDVLIEFYAPWCGHCKKLEPDYLALAKKYKN-EKNLVIAKMDATANDVPHDSYKVEGFPT-IYFAPSNNKQNPIKFEGGERNVEGFSKF-IEEHA 633 HSP 4 Score: 54.299 bits (129), Expect = 2.578e-7 Identity = 25/83 (30.12%), Postives = 44/83 (53.01%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKV--FKYE-GDNSKEALIKFVH 243
FYAPWCG+CK+++P + A A K + + D + D ++ + G+P+++ NK K+E G+ + E KF+
Sbjct: 548 FYAPWCGHCKKLEPDYLALAKKYKNEKNLVIAKMDATANDVPHDSYKVEGFPTIYFAPSNNKQNPIKFEGGERNVEGFSKFIE 630 HSP 5 Score: 52.7582 bits (125), Expect = 8.048e-7 Identity = 39/141 (27.66%), Postives = 60/141 (42.55%), Query Frame = 1
Query: 160 SVFLIQDGNKVFKYEGDN-SKEALIKFV---HNPTQPPPQKDVPWSEETNEIDFLTDANFYE-YLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSN 567
+V ++ +G K + E + E L +FV P K P + + + F E LD+ + V++ FYAPWCGHCK +P + + A + K + +D T S + GFP YF N N
Sbjct: 474 NVAILGEGGKKYAMEPEEFDTEVLREFVVAFKKGKLKPIIKSQPVPKNKGPVKVIVGKTFDEIVLDKKKDVLIEFYAPWCGHCKKLEPDYLALAKKYKNEKNLVIAKMDATANDVPH--DSYKVEGFPTI-YFAPSNNKQN 611
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Cavefish
Match: txndc5 (thioredoxin domain containing 5 [Source:ZFIN;Acc:ZDB-GENE-040426-1951]) HSP 1 Score: 115.546 bits (288), Expect = 8.083e-28 Identity = 89/356 (25.00%), Postives = 156/356 (43.82%), Query Frame = 1
Query: 19 CGYCKQMKP---QFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDF------LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTN----------PANPQLDARRHWRRIPGHE-------AIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
CG+C++++ + A +++ I++ T I GYP++ L + + KY+G ++L ++ Q P++ E + LT NF ++ + S V F+APWCGHCKA P + A + V +DCT ++C+ + G+P +F G+ ++ +R+ + F F+ N PA A IP E ++V L ++NF + S + FY+PWCGHC + P++ + + K L IA VD T E L ++ V G+PTL+ F ++ G R +++ F++K+ D
Sbjct: 2 CGHCQRLQGTWNELADKYNSMETPPIYVVKVDCTKDTKFCSSDHGIRGYPTLKLFKPEQEAVKYQGSRDLQSLETWMLKTLQEEPEEPESEPEPPKVPEPKHGMYELTAGNFKSHISKG-SHFVKFFAPWCGHCKAMAPTWEQLATSFEHSDLVKIGKLDCTQH--YEVCSENQVRGYPTLLFFTDGEKVD--QFRGKRDLESFKEFVDNHLADAASKDQPAAEDAKANE----IPEAEEPAKPESSVVTLTESNFDDIVAKGVS--FIKFYAPWCGHCKNLAPTWEDLSKKEFPGLTDVKIAKVDCTVERSLCNRFSVQGYPTLLIFRAGKQGEEHNGGRDLESLHSFVMKQARD 346 HSP 2 Score: 96.6709 bits (239), Expect = 2.438e-21 Identity = 66/228 (28.95%), Postives = 102/228 (44.74%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEE--TNEID-------------FLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTS-AANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQL 639
F+APWCG+CK M P + AT+ + + D + ++ + GYP++ DG KV ++ G E+ +FV N KD P +E+ NEI LT++NF + + + S I FYAPWCGHCK P + + + V VDCT E +C + G+P F GK E++ R+ + +F+ A +L
Sbjct: 126 FFAPWCGHCKAMAPTWEQLATSFEHSDLVKIGKLDCTQHYEVCSENQVRGYPTLLFFTDGEKVDQFRGKRDLESFKEFVDNHLADAASKDQPAAEDAKANEIPEAEEPAKPESSVVTLTESNFDDIVAKGVSFI-KFYAPWCGHCKNLAPTWEDLSKKEFPGLTDVKIAKVDCTVER--SLCNRFSVQGYPTLLIFRAGKQ--GEEHNGGRDLESLHSFVMKQARDEL 348
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Cavefish
Match: dnajc10 (DnaJ heat shock protein family (Hsp40) member C10 [Source:NCBI gene;Acc:103021499]) HSP 1 Score: 111.309 bits (277), Expect = 3.404e-25 Identity = 89/358 (24.86%), Postives = 151/358 (42.18%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLD---QHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIR---HEEAEKLFQEEKKK 1059
F+APWC C+ + P+ A+ L G+ F + D + + L +NI YP+ +I + + V +YEG +S + +++F+ + P + LT F + + Q E+ +V FYAPWCG C+A P++ A ++ V VDC + +C SE + +P R F N + +S D + T W A V L +F + I+ ++ FY+PWCG C P F A L+ + + VD ++ +PT V F P Y+G + E++ + S++I H+ ++L + K K
Sbjct: 478 FFAPWCPPCRALLPELRKASIQLYGQMKFGTL--DCTIHEGLCSRYNINAYPTT-VIFNKSSVHEYEGHHSADGILEFIQDLVNPA-------------VVTLTPDTFQQLVKRRKQTETWVVDFYAPWCGPCQALLPEWRRMARMLSGVINVG--TVDC--QKHHSLCQSENVRAYPEIRLFPQNSNRRDQYHSYNGWHRDAQSLKT------------WALSSLPRASVDLTPEDFKRKILEGKEHWVLDFYAPWCGPCQHFAPEFEILARMLKGTVRA---GKVDCQAHYQTCQSAGITAYPT-VRFYP------YLGTQRRQQGGEYINSRDSNVISDVIHQRLQQLALKNKSK 793 HSP 2 Score: 75.0998 bits (183), Expect = 1.098e-13 Identity = 55/202 (27.23%), Postives = 82/202 (40.59%), Query Frame = 1
Query: 340 DQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNI--VSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGD 939
D E +V F+APWC C+A P+ A+ Q+ K F +DCT +C+ I +P FN +S EY D + F+ + NP A+V L + F Q + T +V FY+PWCG C A+ P + A L ++ + VD L V +P + F N +
Sbjct: 469 DGKEPWLVDFFAPWCPPCRALLPELRKASIQLYGQMK--FGTLDCTIHE--GLCSRYNINAYPTTVIFN---KSSVHEYEGHHSADGILEFIQDLVNP---------------AVVTLTPDTFQQLVKRRKQTETWVVDFYAPWCGPCQALLPEWRRMARMLSGVIN---VGTVDCQKHHSLCQSENVRAYPEIRLFPQNSN 645
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006474.1 (pep scaffold:Pmarinus_7.0:GL478000:14574:47610:1 gene:ENSPMAG00000005827.1 transcript:ENSPMAT00000006474.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 180.644 bits (457), Expect = 2.434e-50 Identity = 120/356 (33.71%), Postives = 179/356 (50.28%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYE--GDNSKEALIKFVHNPTQPPPQ-KDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAA---NQMAENKKVAFIGVDCT-----DESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLE--REEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTE--THLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMI----KKHSDI 1011
MFYAPWCG CK+MKP F AAT K + G+N + D +K +N+TGYP+ + G F Y+ G +K+ +++++ NP P P +V WSE + + LTD +F +++D+H +V+VMFYAPWCGHCK KP + AA N EN VD T E FA I GFP ++F G+ +Y++ R +D ++++ + P W + L F + + AL+MFY+PWC HC KP F AA +L+ L AAVD T + L +Q V G+PT+ +++ Y G R + F++ K HS++
Sbjct: 178 MFYAPWCGVCKRMKPVFQEAATMAKKTYVLAGMNVHPAEFDGIKSDYNVTGYPTFCYFEKGKFRFNYQSYGAQAKD-IVEWLKNPESPKPTVPEVAWSETDSAVFHLTDEDFQDFVDKHPNVLVMFYAPWCGHCKKLKPDYDEAAKILNNEEENSPGVLAAVDATIHRALAERFA-------IKGFPTMKFFQDGE----EKYTVSDLRSKDKILSWIRD-PQPPPPPEVPWDE--QENDVAHLSVATF-REFLKKKRHALLMFYAPWCFHCKQAKPHFTAAAAQLREDHKVAL-AAVDCTKQDNNELCSQEGVRGYPTMNHYTYGKFVGPYTGGRQEADFINFLLSLRGKSHSEL 516 HSP 2 Score: 130.568 bits (327), Expect = 1.047e-32 Identity = 91/287 (31.71%), Postives = 139/287 (48.43%), Query Frame = 1
Query: 151 GYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFL---TDANFYEYLDQHES-VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARR-HWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSC--LIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
G + +DG +Y+ + ++L+ F+++PT P W EE + D + +D F L + E +++MFYAPWCG CK KP F AA MA+ K G++ F I + +TG+P F YF GK N + S + D V ++ NP +P+ W A+ L +F Q+ V LVMFY+PWCGHC +KP + AA L + ++AAVDAT LA ++ + GFPT+ +F + R+ D I ++
Sbjct: 108 GTSKLMHYKDGEFHVEYDRAETFKSLVAFMYDPTGDPL-----WEEEPDAKDVVHVHSDKEFRLLLKKEERPILMMFYAPWCGVCKRMKPVFQEAAT-MAK-KTYVLAGMNVHPAEFDGIKSDYNVTGYPTFCYFEKGKFRFNYQ-SYGAQAKDIVEWLKNPESPKPTVPEVAWSETDS--AVFHLTDEDF-QDFVDKHPNVLVMFYAPWCGHCKKLKPDYDEAAKILNNEEENSPGVLAAVDATIHRALAERFAIKGFPTMKFFQDGEEKYTVSDLRSKDKILSWI 383 HSP 3 Score: 58.5362 bits (140), Expect = 2.653e-9 Identity = 42/157 (26.75%), Postives = 62/157 (39.49%), Query Frame = 1
Query: 466 VDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNN-FVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAA---VDATTETHLAAQYKVSGFPTLVYF 924
VDC + +C + + +Y + EY VAFM +P L W P + +V + + F + L+MFY+PWCG C MKP F AA T+ + A V + + Y V+G+PT YF
Sbjct: 88 VDCGNSEGRKLCKKMKVEPGGTSKLMHYKDGEFHVEYDRAETFKSLVAFMYDPTGDPL-----WEEEPDAKDVVHVHSDKEFRLLLKKEERPILMMFYAPWCGVCKRMKPVFQEAA----TMAKKTYVLAGMNVHPAEFDGIKSDYNVTGYPTFCYF 235
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Sea Lamprey
Match: pdia5 (protein disulfide isomerase family A, member 5 [Source:ZFIN;Acc:ZDB-GENE-030521-5]) HSP 1 Score: 159.458 bits (402), Expect = 1.311e-42 Identity = 102/340 (30.00%), Postives = 165/340 (48.53%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEG-DNSKEALIKFVHNPTQPPPQKD-VPWSEETNE-IDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATT--ETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHS 1005
MFYAPWCG CK + P F AAT LK + G+N D +KQ F ITGYP+ I+ G +F +E +K+ ++ ++ NP P P + VPWS++ + + LT + F +L H S +V+FYAPWC + +P++ +AA + + + + + G+T P YF+ G+ N S +R + + F+ NP +PQ + W + P ++ L + +++ + T ALVMFY PWC H F AA +L+ L A+D + + + Q V +PT Y+S +Y G R + +M ++
Sbjct: 197 MFYAPWCGVCKTLMPAFQEAATELKETHVLAGMNLHAPEFDGIKQDFRITGYPTFAYIEKGRHLFNFENYQATKQDIVSWLLNPRPPRPPAEMVPWSQDPDTLVHHLTASTFGPFLGTHPSTLVLFYAPWCPVSRKVRPEYEAAAAILNPRPDSPGVLAAVDGATEVGLVEAYGVTTLPSLYYFHEGRPLYNLPMSHDR--NTLLTFLRNPQDPQ-SSELPWEQRPS--SVHHLTSRD-LRDFLKKTKHALVMFYVPWCPHSQQAMLHFTGAAEQLKEDRKMGL-GAIDCSKAGNSEICGQEGVQEYPTFRYYSYGRALERYTGPRGESDFVNYMRRQRG 529 HSP 2 Score: 101.679 bits (252), Expect = 5.394e-23 Identity = 76/253 (30.04%), Postives = 122/253 (48.22%), Query Frame = 1
Query: 175 QDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYL-DQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARR-HWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPS-CLIAAVDATTETHLAAQYKVSGFPTLVYF 924
+DG +Y + +++ F+ +P P +D P + + + TD F + ++ + +MFYAPWCG CK P F AA ++ E +A G++ F I ITG+P F Y G++ N E + + + D V+++ NP P+ A W + P + L + F + +H S LV+FY+PWC ++P + AA L S ++AAVD TE L Y V+ P+L YF
Sbjct: 135 RDGAFHIEYTRPETFKSMTAFLRDPLGAPLWEDSPSARDVKHLT--TDQEFRTLVKNEARPIFIMFYAPWCGVCKTLMPAFQEAATELKETHVLA--GMNLHAPEFDGIKQDFRITGYPTFAYIEKGRHLFNFE-NYQATKQDIVSWLLNPRPPRPPAEMVPWSQDPD-TLVHHLTASTFGPFLGTHPS-TLVLFYAPWCPVSRKVRPEYEAAAAILNPRPDSPGVLAAVDGATEVGLVEAYGVTTLPSLYYF 380
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Sea Lamprey
Match: txndc5 (thioredoxin domain containing 5 [Source:ZFIN;Acc:ZDB-GENE-040426-1951]) HSP 1 Score: 140.198 bits (352), Expect = 1.132e-36 Identity = 92/352 (26.14%), Postives = 163/352 (46.31%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAA---TTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKF----------VHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHE---AIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
MF+APWCG+C++++ + + ++ D + + GYP++ L + G K Y+G + L + VH ++ P D+P E + + LT +F E++ + I F+APWCGHCKA P + A+ + KV VDCT + DIC + G+P +F G+ +Y +R+ D F+ + + ++ E ++++L +NNF + + V FY+PWCGHC + P++ + A K + L IA VD T+E L ++ V G+PTL+ + ++ G R +A+ +++ + D
Sbjct: 63 MFFAPWCGHCQKLQSTWNTLGDKYNEMPEPPAYVA-KVDCTMHAQTCSDHGVKGYPTLKLFKPGQKSILYQGQRDLDTLESWMLQTLNEDAGVHEESERP-SPDIP--EPQHGLYELTSGSFKEHVAEGYHFI-KFFAPWCGHCKALIPAWEQLASTFEYSGKVKIAKVDCT--AHQDICNENQVRGYPTLLWFKDGEKVD--QYRGKRDLDSLKVFVDTQIQIEPEDEVVTQQAGDEEEASSVLVLNENNFDATVADGIT--FVKFYAPWCGHCKNLAPTWEDLAKKEFSGLSKVTIAKVDCTSEAQLCSKQHVRGYPTLLLYVGGKQVGEHEGTRNLEALHSYVLSRARD 403 HSP 2 Score: 93.2041 bits (230), Expect = 1.022e-20 Identity = 58/218 (26.61%), Postives = 103/218 (47.25%), Query Frame = 1
Query: 358 IVMFYAPWCGHCKATKPKFTSAANQMAEN-KKVAFIG-VDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRR----IP-GHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
+MF+APWCGHC+ + + + ++ E + A++ VDCT A C+ G+ G+P + F G+ + Y +R+ D ++M N R IP + L +F +++ + F++PWCGHC A+ P++ A+ + IA VD T + + +V G+PTL++F +Y G+R D++ F+
Sbjct: 61 FIMFFAPWCGHCQKLQSTWNTLGDKYNEMPEPPAYVAKVDCT--MHAQTCSDHGVKGYPTLKLFKPGQKSIL--YQGQRDLDTLESWMLQTLNEDAGVHEESERPSPDIPEPQHGLYELTSGSFKEHVAE--GYHFIKFFAPWCGHCKALIPAWEQLASTFE-YSGKVKIAKVDCTAHQDICNENQVRGYPTLLWFKDGEKVDQYRGKRDLDSLKVFV 271
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Sea Lamprey
Match: pdia4 (protein disulfide isomerase family A, member 4 [Source:ZFIN;Acc:ZDB-GENE-030131-5493]) HSP 1 Score: 130.183 bits (326), Expect = 4.090e-32 Identity = 78/239 (32.64%), Postives = 120/239 (50.21%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKK-VAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKK 999
+E N + L D NF ++ ++V++ FYAPWCGHCK ++ A ++AEN + VD T + ++G+P + G+ +Y +R +D +A + A+P W+ P EA++ L ++NF IV+ LV FY+PWCGHC + P + AA +L P +A VDAT E+ L ++ VSG+PTL F G Y G R I MI++
Sbjct: 53 KEENGVLILKDLNFDFFVAGKDTVLLEFYAPWCGHCKEFASEYEKIAKELAENDPPIPVAKVDAT--VAVHLAERFSVSGYPTIKILKKGQAV---DYDGDRTQDAIIAKVKEVASPD------WK--PPPEAVLTLTESNF-DEIVNDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKNTPPIPLAKVDATVESELGKRFGVSGYPTLKLFR-KGKAYDYNGPREQYGIVSHMIEQ 276 HSP 2 Score: 119.783 bits (299), Expect = 9.144e-29 Identity = 71/240 (29.58%), Postives = 120/240 (50.00%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENK-KVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQN 717
FYAPWCG+CK+ ++ A L I + D + L + F+++GYP++ +++ G V Y+GD +++A+I V P W + LT++NF E ++ + ++V FYAPWCGHCK P++ AA ++++N + VD T ES ++ G++G+P + F GK +Y+ RE+ V+ M A P + +++ N FVQN
Sbjct: 80 FYAPWCGHCKEFASEYEKIAKELAENDPPIPVAKVDATVAVHLAERFSVSGYPTIKILKKGQAV-DYDGDRTQDAIIAKVKEVASP------DWKPPPEAVLTLTESNFDEIVNDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKNTPPIPLAKVDATVES--ELGKRFGVSGYPTLKLFRKGKAY---DYNGPREQYGIVSHMIEQAGPASQELKSMKQL-----------NGFVQN 296 HSP 3 Score: 65.0846 bits (157), Expect = 2.849e-11 Identity = 36/112 (32.14%), Postives = 64/112 (57.14%), Query Frame = 1
Query: 682 IVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYM-GERTDDAITEFMIKKHS 1005
+ ++ F Q ++ ++ L+ FY+PWCGHC +++P + + K + +IA +DAT YK GFPT +YFSP+G +K+ G R +++++F I+KH+
Sbjct: 522 VKVVVGRTFEQMVLDNSKDVLIEFYAPWCGHCKSLEPIYKDLGKKYKG-HKGLVIAKMDATVNDIPHDSYKAEGFPT-IYFSPSGAKDRPIKFADGSRDLESLSKF-IEKHA 630 HSP 4 Score: 48.9062 bits (115), Expect = 2.702e-6 Identity = 23/84 (27.38%), Postives = 41/84 (48.81%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDG--NKVFKY-EGDNSKEALIKFVHN 246
FYAPWCG+CK ++P + KG + D + D ++ G+P+++ G ++ K+ +G E+L KF+
Sbjct: 545 FYAPWCGHCKSLEPIYKDLGKKYKGHKGLVIAKMDATVNDIPHDSYKAEGFPTIYFSPSGAKDRPIKFADGSRDLESLSKFIEK 628
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Sea Lamprey
Match: dnajc10 (DnaJ (Hsp40) homolog, subfamily C, member 10 [Source:ZFIN;Acc:ZDB-GENE-070327-1]) HSP 1 Score: 114.775 bits (286), Expect = 6.479e-27 Identity = 87/352 (24.72%), Postives = 145/352 (41.19%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQH---ESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMG-----ERTDDAITEFMIKKHSDIIRHEEAEK 1035
F+APWC C+ + P+ A+ L G+ F I D + + I YP+ + N + +Y G +S E L+++V + P + LT + + Q E +V FYAPWCG C+A P++ A ++ +V VDC + +C SE + +P R F +S+ F N + ++ + W + L F ++ LV FY+PWCGHC P F AA L+ L + V+ + H+ + G+PT++++ G + G R I E ++++ DI HE +K
Sbjct: 476 FFAPWCPPCRALLPELRKASRLLTGQLKFGTI--DCTIHSKICNLHGIHAYPTTLIFNQSN-IHEYNGQHSAEQLMEYVQDLMSP-------------SVVTLTPETYVSLVKQRKRDEVWMVDFYAPWCGPCQALLPEWKRMARMLSGMVQVG--SVDC--QKHHALCQSEMVHAYPEIRLFPMNPASSDK------------YFKYNGWHRDANSLKGWAFGYLPQTAEDLTPQKFNSEVLEGMEHWLVDFYTPWCGHCQIFAPEFEIAARLLKGKLRT---GKVNCQNDPHVCQSAGIHGYPTVMFYQYQGRSKRSTGGEEINSRNGKDIAENLLRRVGDIPLHENTKK 792 HSP 2 Score: 68.1662 bits (165), Expect = 3.468e-12 Identity = 51/213 (23.94%), Postives = 87/213 (40.85%), Query Frame = 1
Query: 349 ESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVM--FYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPN----GDWVKYMGERTD 969
E ++ F+APWC C+A P+ A+ + ++ F +DCT S IC GI +P FN ++ EY+ + + + ++ + +P ++V L +V + + M FY+PWCG C A+ P + A L ++ + +VD L V +P + F N + KY G D
Sbjct: 470 EPWLIDFFAPWCPPCRALLPELRKASRLL--TGQLKFGTIDCTIHS--KICNLHGIHAYPTTLIFN---QSNIHEYNGQHSAEQLMEYVQDLMSP---------------SVVTLTPETYVSLVKQRKRDEVWMVDFYAPWCGPCQALLPEWKRMARMLSGMVQ---VGSVDCQKHHALCQSEMVHAYPEIRLFPMNPASSDKYFKYNGWHRD 657 HSP 3 Score: 56.225 bits (134), Expect = 1.466e-8 Identity = 53/223 (23.77%), Postives = 86/223 (38.57%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNI--VSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCL-IAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWV 945
++ E+ L F + E V FY+P C HC P + A +M +VA V+C D +C S+GI +P + G +YS +R +DD V F+ + + L NFV T ++ Y G C++ + KL +L + VD +T+ H+ +Q + T+ Y P G W+
Sbjct: 128 DDDAEVLTLDRGEFDAAVSSGELFFVNFYSPRCSHCHELAPMWREFAKEMDGVIRVA--AVNCGDNRM--MCRSKGIGSYPSLYVYRAGMQP--VKYSGDRSKDDLVRFVMKYVTSK---------------VTELWAGNFVNAFEQAGSTETGWLLSYCSDIGDCLSQ-----HTRRKLAGMLDGMANVGWVDCSTQKHICSQMNIEESKTVFY--PPGLWL 322 HSP 4 Score: 49.6766 bits (117), Expect = 1.530e-6 Identity = 45/178 (25.28%), Postives = 76/178 (42.70%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGIN-GDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWC---GHC--KATKPKFTSAANQMAENKKVAFIG-VDCTDESFADICTSEGI 516
FY+P C +C ++ P + A + G +N GD + ++ I YPS+++ + G + KY GD SK+ L++FV T+++ L NF +Q S + +C G C + T+ K + MA +G VDC+ + IC+ I
Sbjct: 155 FYSPRCSHCHELAPMWREFAKEMDGVIRVAAVNCGDNRM---MCRSKGIGSYPSLYVYRAGMQPVKYSGDRSKDDLVRFVMKYV-------------TSKVTELWAGNFVNAFEQAGSTETGWLLSYCSDIGDCLSQHTRRKLAGMLDGMAN------VGWVDCSTQKH--ICSQMNI 308 HSP 5 Score: 48.1358 bits (113), Expect = 5.449e-6 Identity = 28/107 (26.17%), Postives = 52/107 (48.60%), Query Frame = 1
Query: 682 IVILEKNNFVQNIVSHTSGAL--VMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIK 996
++ L++ F + +SG L V FYSP C HC + P + A ++ ++ +AAV+ + + +P+L + VKY G+R+ D + F++K
Sbjct: 133 VLTLDRGEFDAAV---SSGELFFVNFYSPRCSHCHELAPMWREFAKEMDGVI---RVAAVNCGDNRMMCRSKGIGSYPSLYVYRAGMQPVKYSGDRSKDDLVRFVMK 233
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Yeast
Match: PDI1 (Protein disulfide isomerase; multifunctional oxidoreductase of the ER lumen, essential for disulfide bond formation in secretory and cell-surface proteins, processing of non-native disulfide bonds; Ero1p activator; complexes with exomannosidase, Mnl1p to facilitate the recognition of misfolded glycoproteins and the trimming of glycan Man8GlcNAc2 to Man7GlcNAc2 on substrates, thereby accelerating ERAD; PDI1 has a paralog, EUG1, that arose from the whole genome duplication [Source:SGD;Acc:S000000548]) HSP 1 Score: 77.411 bits (189), Expect = 1.620e-15 Identity = 39/125 (31.20%), Postives = 63/125 (50.40%), Query Frame = 1
Query: 265 QKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQL 639
Q++ E++ + TD+ F EY+ H+ V+ F+APWCGHCK P++ AA + E K + +DCT+ D+C I GFP + F ++ +Y R + V FM + P +
Sbjct: 23 QQEAVAPEDSAVVKLATDS-FNEYIQSHDLVLAEFFAPWCGHCKNMAPEYVKAAETLVE-KNITLAQIDCTENQ--DLCMEHNIPGFPSLKIFKNSDVNNSIDYEGPRTAEAIVQFMIKQSQPAV 143 HSP 2 Score: 73.9442 bits (180), Expect = 2.238e-14 Identity = 42/113 (37.17%), Postives = 62/113 (54.87%), Query Frame = 1
Query: 667 PGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYF--SPNGDWVKYMGERTDDAITEFMIKK 999
P A+V L ++F + I SH L F++PWCGHC M P +V AA L + + +A +D T L ++ + GFP+L F S + + Y G RT +AI +FMIK+
Sbjct: 29 PEDSAVVKLATDSFNEYIQSHDL-VLAEFFAPWCGHCKNMAPEYVKAAETL--VEKNITLAQIDCTENQDLCMEHNIPGFPSLKIFKNSDVNNSIDYEGPRTAEAIVQFMIKQ 138 HSP 3 Score: 68.1662 bits (165), Expect = 1.323e-12 Identity = 35/87 (40.23%), Postives = 50/87 (57.47%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPS--VFLIQDGNKVFKYEGDNSKEALIKFVHNPTQP 258
F+APWCG+CK M P++ AA TL K+I + D + DL NI G+PS +F D N YEG + EA+++F+ +QP
Sbjct: 56 FFAPWCGHCKNMAPEYVKAAETLVEKNITLA-QIDCTENQDLCMEHNIPGFPSLKIFKNSDVNNSIDYEGPRTAEAIVQFMIKQSQP 141 HSP 4 Score: 55.4546 bits (132), Expect = 1.549e-8 Identity = 28/86 (32.56%), Postives = 46/86 (53.49%), Query Frame = 1
Query: 742 LVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNG---DWVKYMGERTDDAITEFM 990
LV++Y+PWCGHC + P++ A+ LIA +D T + G+PT+V + P G + V Y G R+ D++ +F+
Sbjct: 398 LVLYYAPWCGHCKRLAPTYQELADTYANATSDVLIAKLDHTEND--VRGVVIEGYPTIVLY-PGGKKSESVVYQGSRSLDSLFDFI 480 HSP 5 Score: 50.8322 bits (120), Expect = 3.542e-7 Identity = 28/104 (26.92%), Postives = 45/104 (43.27%), Query Frame = 1
Query: 340 DQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIG-VDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDAR 648
D + V+V++YAPWCGHCK P + A+ A I +D T+ + I G+P + GK + + Y R D F+ + +D +
Sbjct: 392 DPKKDVLVLYYAPWCGHCKRLAPTYQELADTYANATSDVLIAKLDHTENDVRGVV----IEGYPTIVLYPGGKKSESVVYQGSRSLDSLFDFIKENGHFDVDGK 491 HSP 6 Score: 50.447 bits (119), Expect = 4.796e-7 Identity = 26/82 (31.71%), Postives = 43/82 (52.44%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNK--VFKYEGDNSKEALIKFV 240
++YAPWCG+CK++ P + A T + + I L T++ + I GYP++ L G K Y+G S ++L F+
Sbjct: 400 LYYAPWCGHCKRLAPTYQELADTYANATSDVLI-AKLDHTENDVRGVVIEGYPTIVLYPGGKKSESVVYQGSRSLDSLFDFI 480
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Yeast
Match: MPD1 (Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation [Source:SGD;Acc:S000005814]) HSP 1 Score: 59.6918 bits (143), Expect = 3.797e-10 Identity = 29/66 (43.94%), Postives = 40/66 (60.61%), Query Frame = 1
Query: 739 ALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAV--DATTETHLAAQYKVSGFPTLVYFSP 930
+LV FY+PWCGHC + +F AA +L ++ +AAV D L A+Y V+GFPTL+ F P
Sbjct: 50 SLVEFYAPWCGHCKKLSSTFRKAAKRLDGVVQ---VAAVNCDLNKNKALCAKYDVNGFPTLMVFRP 112 HSP 2 Score: 52.373 bits (124), Expect = 9.183e-8 Identity = 23/75 (30.67%), Postives = 39/75 (52.00%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEAL 228
FYAPWCG+CK++ F AA L G +N DL+ L +++ G+P++ + + DN+K++
Sbjct: 54 FYAPWCGHCKKLSSTFRKAAKRLDGVVQVAAVNCDLNKNKALCAKYDVNGFPTLMVFRPPKIDLSKPIDNAKKSF 128 HSP 3 Score: 49.6766 bits (117), Expect = 6.515e-7 Identity = 23/62 (37.10%), Postives = 30/62 (48.39%), Query Frame = 1
Query: 358 IVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYF 543
+V FYAPWCGHCK F AA ++ +VA V+C +C + GFP F
Sbjct: 51 LVEFYAPWCGHCKKLSSTFRKAAKRLDGVVQVA--AVNCDLNKNKALCAKYDVNGFPTLMVF 110
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Yeast
Match: EUG1 (Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER [Source:SGD;Acc:S000002926]) HSP 1 Score: 58.5362 bits (140), Expect = 1.695e-9 Identity = 28/102 (27.45%), Postives = 51/102 (50.00%), Query Frame = 1
Query: 310 LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFM 615
LT+ F +++ H V+V F+APWC H + +P AA+ + E+ V + +DC E+ + +C + I +P + F G+ Y + D+ +M
Sbjct: 38 LTEKKFKSFIESHPLVLVEFFAPWCLHSQILRPHLEEAASILKEH-NVPVVQIDC--EANSMVCLQQTINTYPTLKIFKNGRIFDGQVYRGVKITDEITQYM 136 HSP 2 Score: 54.299 bits (129), Expect = 3.730e-8 Identity = 38/121 (31.40%), Postives = 59/121 (48.76%), Query Frame = 1
Query: 643 ARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNG---DWVKYMGERTDDAITEFMIK 996
A R PG + +V+ EK F I SH LV F++PWC H ++P AA+ L+ + + +D + + Q ++ +PTL F NG D Y G + D IT++MI+
Sbjct: 23 AENSARATPGSDLLVLTEKK-FKSFIESHPL-VLVEFFAPWCLHSQILRPHLEEAASILKE--HNVPVVQIDCEANSMVCLQQTINTYPTLKIFK-NGRIFDGQVYRGVKITDEITQYMIQ 138
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Yeast
Match: EPS1 (ER protein with chaperone and co-chaperone activity; involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family [Source:SGD;Acc:S000001267]) HSP 1 Score: 46.9802 bits (110), Expect = 6.645e-6 Identity = 34/110 (30.91%), Postives = 47/110 (42.73%), Query Frame = 1
Query: 226 LIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKK---VAFIGVDCTDESFADICTSEGITGFPIFRYFN 546
L+KF +PP P L NF E L + +I FY+P+C HCK P + + E K + F V+C + AD+C E I FP R +N
Sbjct: 19 LLKFTIAAAEPPEGFPEP----------LNPTNFKEELSKGLHIID-FYSPYCPHCKHLAPVWMETWEEFKEESKTLNITFSQVNCIES--ADLCGDENIEYFPEIRLYN 115
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Nematostella
Match: EDO41376 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4E6]) HSP 1 Score: 205.297 bits (521), Expect = 1.667e-59 Identity = 119/338 (35.21%), Postives = 178/338 (52.66%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQK--DVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFI-GVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSL---EREEDDFVAFMTN--PANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
MFYAPWCGYCK+ KP+FAAAAT K +++ G++ D ++ +NITG+P+ + G +KY G + K+AL++++ +P+ P K + PWS+ +E+ L D F +++ ++ SV+VMFYAPWCGHCKA KP++ AA + E + + VD T E A + + G+P G + + E++ ER+ D V FM + + W IP + L F ++ V LVMFY+PWCGHC KP ++AA K AAVD T E + Q+ V G+PT YF+ + KY R +FM
Sbjct: 141 MFYAPWCGYCKRFKPEFAAAATEHKDEAVLAGMDVDTEDGYSVRVHYNITGFPTTIYFELGQPKYKYSGKHEKDALVQWMKDPSAVAPVKEDEKPWSDTPSEVVHLRDDMFDDFVAKNPSVLVMFYAPWCGHCKAMKPEYVDAAQTLKEQEIPGVLAAVDATKE--AALGKRFKVEGYPT------GTSYMDGEFAFDVNERKGDSIVNFMKDPKEPPRPPPPEQEWSEIPSE--VYHLSDTTF-KSFVKKKKHVLVMFYAPWCGHCKKAKPELMSAA-KHHKDKNKIAYAAVDCTKEMAVCQQFGVEGYPTFRYFNYGKNDFKYTSGREAKDFIQFM 466 HSP 2 Score: 159.458 bits (402), Expect = 1.021e-42 Identity = 105/322 (32.61%), Postives = 168/322 (52.17%), Query Frame = 1
Query: 49 FAAAATTLKGKSIFIGIN-GDLSTTDDLKQAFNITGYP-SVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEE--TNEIDFLTDANFYEYLDQHES--VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPA--NPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
A +KGK IN GD + L + F ++ P ++ ++G+ Y+ ++ ++++ F++NPT D PW EE ++++ L A L E V++MFYAPWCG+CK KP+F +AA + + +A G+D E + ITGFP YF G+ +YS + E+D V +M +P+ P + + W P V+ +++ + V+ LVMFY+PWCGHC AMKP +V+AA L+ ++AAVDAT E L ++KV G+PT + +G++ + ER D+I FM
Sbjct: 38 LGEVAMAIKGKGTVAFINCGD---SKKLCKKFKVSPKPLALKHYKEGDFNKDYDRLDTFKSMMTFMNNPTG-----DAPWEEEPGSSDVVHLEKAGELSKLLTREKKPVLIMFYAPWCGYCKRFKPEFAAAATEHKDEAVLA--GMDVDTEDGYSVRVHYNITGFPTTIYFELGQ--PKYKYSGKHEKDALVQWMKDPSAVAPVKEDEKPWSDTPSE---VVHLRDDMFDDFVAKNPSVLVMFYAPWCGHCKAMKPEYVDAAQTLKEQEIPGVLAAVDATKEAALGKRFKVEGYPTGTSYM-DGEFAFDVNERKGDSIVNFM 343 HSP 3 Score: 62.003 bits (149), Expect = 2.935e-10 Identity = 30/87 (34.48%), Postives = 44/87 (50.57%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPP 261
MFYAPWCG+CK+ KP+ +AA K K+ D + + Q F + GYP+ G FKY + I+F+ +P + P
Sbjct: 387 MFYAPWCGHCKKAKPELMSAAKHHKDKNKIAYAAVDCTKEMAVCQQFGVEGYPTFRYFNYGKNDFKYTSGREAKDFIQFMDDPREGP 473
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Nematostella
Match: EDO41377 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4E7]) HSP 1 Score: 166.392 bits (420), Expect = 3.150e-46 Identity = 106/327 (32.42%), Postives = 169/327 (51.68%), Query Frame = 1
Query: 19 CGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVP-WSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMA-ENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
C +C++MKP F AA L GK + + D + + + +I GYP++ I++G FKY G + EAL+ F+ +P +P P WS++ +++ FLTD + E++ HE+V+VM++APWCGHC KP + AA + E+ VDCT D+ + G+P + + GK EY +R E D V FM +N A + L+ ++F +++T LVMFY+PWCGHC KP + AA + P+ + A +D T + + +V+G+PTL Y+ V+Y G+R + + FM
Sbjct: 26 CPHCQKMKPVFEKAAKQL-GKDVKGALAAVDCTESKNTCNQRDIKGYPTLQYIREGEFQFKYTGRRTAEALVSFMKDPKKPAPPPPPADWSKDDSKVVFLTDESHDEFIKSHENVLVMYFAPWCGHCNEMKPNYYKAAQVLHDEDANCNLAAVDCTKHK--DVAKKVALAGYPTVKLYKNGKVA--KEYEGDRSEKDLVLFMRTASNTAKAASAE----EDSSLVKQLDGSDF-WGYLNNTEHVLVMFYAPWCGHCKNAKPKYEKAAETFKD-QPNRVFAKLDCTKFGDVCDKEEVNGYPTLRYYLYGKFVVEYDGDRVTEDLISFM 341 HSP 2 Score: 152.525 bits (384), Expect = 2.890e-41 Identity = 78/215 (36.28%), Postives = 117/215 (54.42%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLD 642
M++APWCG+C +MKP + AA L + + D + D+ + + GYP+V L ++G +YEGD S++ L+ F+ T K E+++ + L ++F+ YL+ E V+VMFYAPWCGHCK KPK+ AA + F +DCT F D+C E + G+P RY+ YGK EY +R +D ++FM P P D
Sbjct: 142 MYFAPWCGHCNEMKPNYYKAAQVLHDEDANCNLAAVDCTKHKDVAKKVALAGYPTVKLYKNGKVAKEYEGDRSEKDLVLFMR--TASNTAKAASAEEDSSLVKQLDGSDFWGYLNNTEHVLVMFYAPWCGHCKNAKPKYEKAAETFKDQPNRVFAKLDCT--KFGDVCDKEEVNGYPTLRYYLYGKFV--VEYDGDRVTEDLISFMEEPPLPLSD 350 HSP 3 Score: 69.3218 bits (168), Expect = 9.167e-13 Identity = 35/97 (36.08%), Postives = 51/97 (52.58%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEE 291
MFYAPWCG+CK KP++ AA T K + + D + D+ + GYP++ G V +Y+GD E LI F+ P P P D+P ++
Sbjct: 262 MFYAPWCGHCKNAKPKYEKAAETFKDQPNRVFAKLDCTKFGDVCDKEEVNGYPTLRYYLYGKFVVEYDGDRVTEDLISFMEEP--PLPLSDIPKDQQ 356
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Nematostella
Match: EDO32930 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STM8]) HSP 1 Score: 149.058 bits (375), Expect = 3.091e-38 Identity = 90/260 (34.62%), Postives = 137/260 (52.69%), Query Frame = 1
Query: 286 EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKK-VAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM---IKKHSDIIRHEEAEKLFQEEK 1053
+E +++ L NF ++++ ++V FYAPWCGHCK+ P++ AA +M N V F +D T S DI ++G+P + F G + EY REE V +M ++P +W+ P A + L K NF + +V+ S LV F++PWCGHC + P + AA +LQ P +A VDAT E+ LA +Y+V G+PTL F G +Y G+R I +M + S I+ +A + F +EK
Sbjct: 58 KEEDDVLVLNSKNFDRVIEENNIILVEFYAPWCGHCKSLAPEYAKAAKKMKLNDPPVPFAKMDATVAS--DIAQRFDVSGYPTLKIFRKG---TPYEYEGPREESGIVEYMKKQSDP------NWK--PPPVAALTLTKENFTE-VVNRESLMLVEFFAPWCGHCKQLAPEYEKAAQELQKNDPPIPLAIVDATIESELAQKYEVQGYPTLKVFR-KGKATEYKGQRDQYGIASYMRSQVGPSSRILSSLKAVQDFMKEK 302 HSP 2 Score: 119.398 bits (298), Expect = 2.277e-28 Identity = 82/318 (25.79%), Postives = 142/318 (44.65%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGI-NGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENK-KVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKY 951
FYAPWCG+CK + P++A AA +K + D + D+ Q F+++GYP++ + + G ++YEG + +++++ Q D W LT NF E +++ ++V F+APWCGHCK P++ AA ++ +N + VD T ES ++ + G+P + F GK T EY +R++ ++M + P + IL VQ+ + +M + + G M S++ A N ++ P DA + H + ++V F P KY
Sbjct: 85 FYAPWCGHCKSLAPEYAKAAKKMKLNDPPVPFAKMDATVASDIAQRFDVSGYPTLKIFRKGTP-YEYEGPREESGIVEYMKK------QSDPNWKPPPVAALTLTKENFTEVVNRESLMLVEFFAPWCGHCKQLAPEYEKAAQELQKNDPPIPLAIVDATIES--ELAQKYEVQGYPTLKVFRKGKAT---EYKGQRDQYGIASYMRSQVGP---------------SSRILSSLKAVQDFMKEKDDVTIMGF--FDGEDDKMLESYLEANNDVRDDYP--FAHTFDAAAKKHFGIKKS-----SIVLFQPERFLSKY 366 HSP 3 Score: 61.2326 bits (147), Expect = 7.358e-10 Identity = 32/105 (30.48%), Postives = 49/105 (46.67%), Query Frame = 1
Query: 340 DQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNS-EYSLEREEDDFVAFMTNPANPQLDARR 651
D + V++ FYAPWCGHCKA +P F +K + +D T D+ ++ + GFP YF K+ N ++ RE D + F+ A L +
Sbjct: 541 DPKKDVLIEFYAPWCGHCKALEPTFKKLGKHFRNDKNIVIAKIDATAN---DVPSTYAVEGFPTI-YFATSKDKKNPIKFDGGRELKDLIKFVEEKATVSLSKEK 641 HSP 4 Score: 60.077 bits (144), Expect = 1.840e-9 Identity = 35/122 (28.69%), Postives = 64/122 (52.46%), Query Frame = 1
Query: 676 EAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW---VKYMGERTDDAITEFMIKKHSDIIRHEEAE 1032
E + ++ F + + L+ FY+PWCGHC A++P+F + + +IA +DAT + + Y V GFPT +YF+ + D +K+ G R + +F+ +K + + E+A+
Sbjct: 525 EPVTVVVGKTFDEIVNDPKKDVLIEFYAPWCGHCKALEPTFKKLGKHFRN-DKNIVIAKIDATA-NDVPSTYAVEGFPT-IYFATSKDKKNPIKFDGGRELKDLIKFVEEKATVSLSKEKAK 643 HSP 5 Score: 52.7582 bits (125), Expect = 3.529e-7 Identity = 24/82 (29.27%), Postives = 42/82 (51.22%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLI--QDGNKVFKYEGDNSKEALIKFVH 243
FYAPWCG+CK ++P F + + D +T +D+ + + G+P+++ +D K++G + LIKFV
Sbjct: 550 FYAPWCGHCKALEPTFKKLGKHFRNDKNIVIAKID-ATANDVPSTYAVEGFPTIYFATSKDKKNPIKFDGGRELKDLIKFVE 630
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Nematostella
Match: EDO47340 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMG9]) HSP 1 Score: 130.183 bits (326), Expect = 2.863e-33 Identity = 75/231 (32.47%), Postives = 119/231 (51.52%), Query Frame = 1
Query: 310 LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSP-NGDWVKYMGERTDDAITEFMIKK 999
LT NF E ++ + +V FYAPWCGHCK P + ++ V VD + D+ + + GFP +YF G +T+ EY+ R+ +DF+ F+ + R IP A+ L+++NF + + + + LV F++PWCGHC + P + + P+C+IA VDA + L +Y VSG+PTL +FS N D +Y R + + +FM +K
Sbjct: 27 LTKDNFDEVVNGEKFALVEFYAPWCGHCKQLAPTYEQLGEAYTQSSDVIIAKVDADGDR--DLGSRFDVKGFPTIKYFPKG-STTPEEYNGGRDINDFIKFIEE----KTGVRGRVPVIP--SAVADLDESNFDKIVKNPDNNVLVEFFAPWCGHCKNLAPVYEKVGEAFKN-EPNCVIAKVDADAHSALGQKYGVSGYPTLKFFSKTNKDGEEYSSGRDEQSFVDFMNEK 247 HSP 2 Score: 92.8189 bits (229), Expect = 1.663e-20 Identity = 59/206 (28.64%), Postives = 92/206 (44.66%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKV-FKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHE-SVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFM 615
FYAPWCG+CKQ+ P + S I D DL F++ G+P++ G+ +Y G IKF+ T + V S + L ++NF + + + +V+V F+APWCGHCK P + VD ++ + + G++G+P ++F+ N EYS R+E FV FM
Sbjct: 46 FYAPWCGHCKQLAPTYEQLGEAYTQSSDVIIAKVDADGDRDLGSRFDVKGFPTIKYFPKGSTTPEEYNGGRDINDFIKFIEEKTGVRGRVPVIPSAVAD----LDESNFDKIVKNPDNNVLVEFFAPWCGHCKNLAPVYEKVGEAFKNEPNCVIAKVDA--DAHSALGQKYGVSGYPTLKFFSK-TNKDGEEYSSGRDEQSFVDFM 244
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Nematostella
Match: EDO34590 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNX3]) HSP 1 Score: 129.028 bits (323), Expect = 7.825e-33 Identity = 97/355 (27.32%), Postives = 143/355 (40.28%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTL-KGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVP---WSEETNEIDFLTDANFYEYLDQHESV---IVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDF-------VAFMTNPANPQLDARRHWRRIP-------GHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKH 1002
MFY PWC +CK M P + A K K D ++ +L NI YP++ L DG+ + +Y G + E + FV P K S + LT F D+H + V FYAPWC HC P + A +N + +DCT + C+ G+ GFP + F G+ YS R +D +A + D +P + I + NN + V FY+PWC HC + P + ANK + IA VD T E L + ++G+PTL+ F +Y G R D++ F+++ H
Sbjct: 9 MFYGPWCEHCKNMMPAWEALGEQYSKEKRDLTIAKVDCTSDVNLCVKQNIRAYPTMKLYYDGD-IKRYTGRRNAEDMKVFVDKIVLKPEGKSKDSEGLSTSEAGVHILTKNTF----DKHIELGLHFVKFYAPWCIHCIKLAPIWERLAEDFKDNADITISKIDCT--AHGSKCSQHGVNGFPTLKLFKNGREV--DRYSGMRSLEDLKNYVKLKIAEHGLLSTVTTDKSETAEEVPPTDTDMDAADLIKPYQLNNQNFDTTVSLGTTFVKFYAPWCRHCKILAPVWDQLANKCADQVAGPKIAKVDCTKEESLCQSFGINGYPTLMLFKDGVQKKEYSGNRDLDSLYRFIMQNH 354 HSP 2 Score: 91.2781 bits (225), Expect = 5.628e-20 Identity = 57/212 (26.89%), Postives = 95/212 (44.81%), Query Frame = 1
Query: 361 VMFYAPWCGHCKATKPKFTSAANQMAENKK-VAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPA-NPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
VMFY PWC HCK P + + Q ++ K+ + VDCT + ++C + I +P + + G Y+ R +D F+ P+ ++ + IL KN F ++I V FY+PWC HCI + P + A + I+ +D T +Q+ V+GFPTL F + +Y G R+ + + ++
Sbjct: 8 VMFYGPWCEHCKNMMPAWEALGEQYSKEKRDLTIAKVDCTSD--VNLCVKQNIRAYPTMKLYYDG---DIKRYTGRRNAEDMKVFVDKIVLKPEGKSKDSEGLSTSEAGVHILTKNTFDKHI--ELGLHFVKFYAPWCIHCIKLAPIWERLAEDFKD-NADITISKIDCTAHGSKCSQHGVNGFPTLKLFKNGREVDRYSGMRSLEDLKNYV 211 HSP 3 Score: 54.299 bits (129), Expect = 7.556e-8 Identity = 26/85 (30.59%), Postives = 42/85 (49.41%), Query Frame = 1
Query: 742 LVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIK 996
VMFY PWC HC M P++ + IA VD T++ +L + + +PT+ + +GD +Y G R + + F+ K
Sbjct: 7 FVMFYGPWCEHCKNMMPAWEALGEQYSKEKRDLTIAKVDCTSDVNLCVKQNIRAYPTMKLYY-DGDIKRYTGRRNAEDMKVFVDK 90
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Medaka
Match: pdia5 (protein disulfide isomerase family A member 5 [Source:NCBI gene;Acc:101175203]) HSP 1 Score: 189.119 bits (479), Expect = 2.091e-52 Identity = 123/361 (34.07%), Postives = 185/361 (51.25%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYE--GDNSKEALIKFVHNPTQPPPQ-KDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSE--GITGFPIFRYFNYGKNTSNSEYSLE--REEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATT-ETH-LAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKK 1056
MFYAPWCG CK+M+P F AAT KG+ + G+N S D LKQ +++ GYP+ + G ++ YE G +K+ + ++ NP P P+ +VPWSE + + LTD F +L++H + +VMFYAPWCGHCK KP++ AA + NK GV ++ E I+GFP +YF G+ Y+L R ++ V +M NP P W P ++ L +F + + ALVMFY+PWC HC P F AA + + AAVD T + H + Q V G+PT +++ +Y G+R + T FM +R + EK+ + +++
Sbjct: 214 MFYAPWCGVCKRMQPIFQQAATEAKGQFVLAGMNVHPSEFDGLKQEYSVKGYPTFCYFEKGKFLYHYENYGATAKD-ITDWLKNPQPPQPKTPEVPWSEMDSAVFHLTDDTFDSFLEEHPAALVMFYAPWCGHCKKMKPEYDEAAEIL--NKDADSPGVLAAVDATVHKALGERFKISGFPTVKYFEKGEE----RYTLPHLRTKNMIVEYMHNPQAPP-PPELSWEDKPS--SVSHLGSEDF-REALKKKKHALVMFYAPWCPHCKNAVPHFTTAAELFKEDR-KIVFAAVDCTKGQNHEVCKQEGVEGYPTFNHYNYGKFVERYNGDRGEAGFTGFMRS-----LRGRDQEKVVKRKEE 557 HSP 2 Score: 135.191 bits (339), Expect = 2.184e-33 Identity = 96/320 (30.00%), Postives = 151/320 (47.19%), Query Frame = 1
Query: 61 ATTLKGKSIFIGINGDLSTTDDLKQAFNI-----TGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHES-VIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHE---AIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPS-CLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
A T+KG+ +N S L + + G + +DG +Y + ++++ F+ +P+ PP ++ P +++ I+ T+ F + L + E V++MFYAPWCG CK +P F AA + + G++ F + + G+P F YF GK + E + D ++ NP PQ +P E A+ L + F + H + ALVMFY+PWCGHC MKP + AA L S ++AAVDAT L ++K+SGFPT+ YF + RT + I E+M
Sbjct: 109 AQTVKGQGTIAWVNCGDSEGRKLCKKVKVDPSSKQGGIELLHYKDGTFHTEYNRPTTIKSMVAFLKDPSGPPLWEENPEAKDVVHIE--TEKEFRKLLKKEEKPVLMMFYAPWCGVCKRMQPIFQQAATEA--KGQFVLAGMNVHPSEFDGLKQEYSVKGYPTFCYFEKGKFLYHYE-NYGATAKDITDWLKNPQPPQPKT----PEVPWSEMDSAVFHLTDDTFDSFLEEHPA-ALVMFYAPWCGHCKKMKPEYDEAAEILNKDADSPGVLAAVDATVHKALGERFKISGFPTVKYFEKGEERYTLPHLRTKNMIVEYM 418
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Medaka
Match: txndc5 (thioredoxin domain containing 5 [Source:NCBI gene;Acc:101160445]) HSP 1 Score: 144.821 bits (364), Expect = 1.612e-37 Identity = 97/366 (26.50%), Postives = 168/366 (45.90%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKP---QFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWS------EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDA-------------RRHWRRIPGHEA--------IVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
MFYAPWCG+C++++P + A ++ +++ + T + GYP++ L + + KY+G ++L ++ Q P + E + LT NF E++ + + V F+APWCGHCKA P + A + ++ V VDCT ++C+ G+ G+P +F G+ +Y +R+ D F F+ N QL A ++ IP E +++L +++F + + V FY+PWCGHC + P++ + + K L IA VD T E L +Y V G+PTL+ F ++ G R +++ F++K+ D
Sbjct: 57 MFYAPWCGHCQRLQPTWNELAEKYNSMDEPPVYVVKVDCVQDTKFCSNVHGVRGYPTLLLFKPDQEAVKYQGPRDLQSLETWMLKTLQAEPSEPESELEPPKAPEPKQGMYELTALNFKEHISKG-AHFVKFFAPWCGHCKAMAPTWEQLATTLEHSEDVKIGKVDCTQHY--EVCSDNGVRGYPTLLFFYNGQK--KEQYKGKRDLDSFKDFVDN----QLKALQTEEHEHELKEEQQAANEIPTEEPAKEEAESNVLVLTESDFDEAVAK--GFTFVKFYAPWCGHCKNLAPTWDDLSKKEFPGLTDVKIAKVDCTVERTLCNKYSVRGYPTLIIFRAGEQGDEHHGGRDLESLHNFVMKQTRD 411 HSP 2 Score: 84.7297 bits (208), Expect = 3.451e-17 Identity = 61/218 (27.98%), Postives = 98/218 (44.95%), Query Frame = 1
Query: 358 IVMFYAPWCGHCKATKPKFTSAA---NQMAENKKVAFIGVDCTDESFADICTS-EGITGFPIFRYFNYGKNTSNSE--YSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGA-LVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFM 990
VMFYAPWCGHC+ +P + A N M E V + VDC ++ C++ G+ G+P F + + L+ E + + + + + + L NF ++I + GA V F++PWCGHC AM P++ A L+ I VD T + + V G+PTL++F +Y G+R D+ +F+
Sbjct: 55 FVMFYAPWCGHCQRLQPTWNELAEKYNSMDE-PPVYVVKVDCVQDT--KFCSNVHGVRGYPTLLLFKPDQEAVKYQGPRDLQSLETWMLKTLQAEPSEPESELEPPKAPEPKQGMYELTALNFKEHI---SKGAHFVKFFAPWCGHCKAMAPTWEQLATTLEH-SEDVKIGKVDCTQHYEVCSDNGVRGYPTLLFFYNGQKKEQYKGKRDLDSFKDFV 265 HSP 3 Score: 68.9366 bits (167), Expect = 5.045e-12 Identity = 32/91 (35.16%), Postives = 52/91 (57.14%), Query Frame = 1
Query: 730 TSGALVMFYSPWCGHCIAMKPSFVNAANKLQTL-LPSCLIAAVDATTETHLAAQ-YKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIK 996
T+ VMFY+PWCGHC ++P++ A K ++ P + VD +T + + V G+PTL+ F P+ + VKY G R ++ +M+K
Sbjct: 51 TAPHFVMFYAPWCGHCQRLQPTWNELAEKYNSMDEPPVYVVKVDCVQDTKFCSNVHGVRGYPTLLLFKPDQEAVKYQGPRDLQSLETWMLK 141
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Medaka
Match: pdia4 (protein disulfide isomerase family A member 4 [Source:NCBI gene;Acc:101168751]) HSP 1 Score: 139.428 bits (350), Expect = 1.591e-34 Identity = 87/258 (33.72%), Postives = 129/258 (50.00%), Query Frame = 1
Query: 295 NEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKK-VAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKKKAD 1065
N + LTD NF +++ ++V+V FYAPWCGHCK P++ A + +N + VD T S + + ++G+P + KN +Y R E V + A+P W+ P +A ++L + NF + V++ LV FY+PWCGHC + P + AA +L P +A VDAT ET LA +Y V+GFPTL F G +Y G R + I E M ++ + +A K QE K D
Sbjct: 58 NGVLVLTDNNFDTFMEGKDTVLVEFYAPWCGHCKQFAPEYEKIAQTLKDNDPPIPVAKVDATQAS--QLASKFDVSGYPTIKIL---KNGEPVDYDGARTEKAIVERVKEVAHPD------WK--PPPDATLVLTQENF-DDTVNNADIILVEFYAPWCGHCKRLAPEYEKAAKELSKRTPPIPLAKVDATVETELAKRYGVNGFPTLKIFR-KGRAFEYNGPRENYGIVEHMGEQAGPPSKQVQAVKQVQELIKDGD 300 HSP 2 Score: 120.553 bits (301), Expect = 2.888e-28 Identity = 86/344 (25.00%), Postives = 153/344 (44.48%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAEN-KKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP---QLDARRHWRRI--PGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHC---IAMKPSFVNAANKLQTLLPSCLI------------------AAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKY 951
FYAPWCG+CKQ P++ A TLK I + D + L F+++GYP++ ++++G V Y+G +++A+++ V P W + LT NF + ++ + ++V FYAPWCGHCK P++ AA ++++ + VD T E+ ++ G+ GFP + F G+ EY+ RE V M A P Q+ A + + + G +A+++ I S+ + Y+ C + + SF + KL P ++ D+T+ + + +K P + + P+ D +Y
Sbjct: 82 FYAPWCGHCKQFAPEYEKIAQTLKDNDPPIPVAKVDATQASQLASKFDVSGYPTIKILKNGEPV-DYDGARTEKAIVERVKEVAHP------DWKPPPDATLVLTQENFDDTVNNADIILVEFYAPWCGHCKRLAPEYEKAAKELSKRTPPIPLAKVDATVET--ELAKRYGVNGFPTLKIFRKGRAF---EYNGPRENYGIVEHMGEQAGPPSKQVQAVKQVQELIKDGDDAVIV--------GIFSNEQDSAYELYTEACNTLREDFSFRHSFSSDVAKLLKASPGQIVIVQPEKFRSKYEPASHTLTVKDSTSASEIQEFFKKHSIPLVGHRKPSNDAKRY 405 HSP 3 Score: 62.3882 bits (150), Expect = 8.386e-10 Identity = 42/147 (28.57%), Postives = 73/147 (49.66%), Query Frame = 1
Query: 598 DFV-AFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDW--VKY-MGERTDDAITEFMIKKHSDIIRHEE 1026
DFV AF P + ++ + G +V+ F + ++ L+ FY+PWCGHC ++P +++ K + + +IA +DAT YK GFPT+ NG VK+ G+RT +A++ F+ K + + + +E
Sbjct: 496 DFVMAFKKGKLKPIIKSQPVPKSNTGPVKVVV--GKTFDEIVMDTQKDVLIEFYAPWCGHCKKLEPDYLSLGKKYKK-EKNLVIAKMDATANDIPNDNYKAEGFPTIYLAPANGKQSPVKFEGGDRTVEALSNFLEKHATKLQQKDE 639 HSP 4 Score: 51.2174 bits (121), Expect = 2.959e-6 Identity = 28/93 (30.11%), Postives = 45/93 (48.39%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNK--VFKYE-GDNSKEALIKFVHNPTQPPPQKD 273
FYAPWCG+CK+++P + + K + + D + D + G+P+++L K K+E GD + EAL F+ QKD
Sbjct: 546 FYAPWCGHCKKLEPDYLSLGKKYKKEKNLVIAKMDATANDIPNDNYKAEGFPTIYLAPANGKQSPVKFEGGDRTVEALSNFLEKHATKLQQKD 638 HSP 5 Score: 50.0618 bits (118), Expect = 5.408e-6 Identity = 32/115 (27.83%), Postives = 51/115 (44.35%), Query Frame = 1
Query: 169 LIQDGNKVFKYEGDN-SKEALIKFVHN----------PTQPPPQKDVPWSEETNEIDFLTDANFYE-YLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCT 477
++ +G K F E D+ E L FV +QP P+ + T + + F E +D + V++ FYAPWCGHCK +P + S + + K + +D T
Sbjct: 474 IMAEGGKKFAMEPDDFDSEVLRDFVMAFKKGKLKPIIKSQPVPKSN------TGPVKVVVGKTFDEIVMDTQKDVLIEFYAPWCGHCKKLEPDYLSLGKKYKKEKNLVIAKMDAT 582
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Medaka
Match: txndc5 (thioredoxin domain containing 5 [Source:NCBI gene;Acc:101160445]) HSP 1 Score: 122.094 bits (305), Expect = 3.413e-30 Identity = 85/316 (26.90%), Postives = 145/316 (45.89%), Query Frame = 1
Query: 142 NITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWS------EETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDA-------------RRHWRRIPGHEA--------IVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
+ GYP++ L + + KY+G ++L ++ Q P + E + LT NF E++ + + V F+APWCGHCKA P + A + ++ V VDCT ++C+ G+ G+P +F G+ +Y +R+ D F F+ N QL A ++ IP E +++L +++F + + + V FY+PWCGHC + P++ + + K L IA VD T E L +Y V G+PTL+ F ++ G R +++ F++K+ D
Sbjct: 25 GVRGYPTLLLFKPDQEAVKYQGPRDLQSLETWMLKTLQAEPSEPESELEPPKAPEPKQGMYELTALNFKEHISKG-AHFVKFFAPWCGHCKAMAPTWEQLATTLEHSEDVKIGKVDCTQHY--EVCSDNGVRGYPTLLFFYNGQK--KEQYKGKRDLDSFKDFVDN----QLKALQTEEHEHELKEEQQAANEIPTEEPAKEEAESNVLVLTESDFDEAVAKGFT--FVKFYAPWCGHCKNLAPTWDDLSKKEFPGLTDVKIAKVDCTVERTLCNKYSVRGYPTLIIFRAGEQGDEHHGGRDLESLHNFVMKQTRD 329 HSP 2 Score: 80.8777 bits (198), Expect = 3.066e-16 Identity = 59/211 (27.96%), Postives = 94/211 (44.55%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLK-GKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHN------------------------PTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTS-AANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKN 558
F+APWCG+CK M P + ATTL+ + + IG D + ++ + GYP++ +G K +Y+G ++ FV N PT+ P +++ E+N + LT+++F E + + V FYAPWCGHCK P + + + V VDCT E +C + G+P F G+
Sbjct: 105 FFAPWCGHCKAMAPTWEQLATTLEHSEDVKIG-KVDCTQHYEVCSDNGVRGYPTLLFFYNGQKKEQYKGKRDLDSFKDFVDNQLKALQTEEHEHELKEEQQAANEIPTEEPAKEEA----ESN-VLVLTESDFDEAVAKG-FTFVKFYAPWCGHCKNLAPTWDDLSKKEFPGLTDVKIAKVDCTVER--TLCNKYSVRGYPTLIIFRAGEQ 306
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Medaka
Match: pdia6 (protein disulfide isomerase family A member 6 [Source:NCBI gene;Acc:101171545]) HSP 1 Score: 103.99 bits (258), Expect = 2.130e-23 Identity = 72/254 (28.35%), Postives = 110/254 (43.31%), Query Frame = 1
Query: 292 TNEIDFLTDANFYEYLDQHESV-IVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDAR-------------RHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCL-IAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
++++ LT +NF + Q +S+ +V FYAPWCGHC+ P + AA + KV + D + G+ GFP + F KN EY R V N + R + +V L +NF + ++ LV F++PWCGHC ++P + AA ++ + + AVDAT L+++Y V GFPT+ F + Y G RT I E + SD
Sbjct: 24 SDDVVELTPSNFNREVMQSDSLWLVEFYAPWCGHCRNLAPDWKKAATALKGIVKVGAVDAD----EHKSLGGQYGVRGFPTIKIFGANKNKPE-EYQGARSSQAIVDGAMNALRSLVKDRLSGKSGSSGYSRQSDSGSSGSSKDVVELTDDNFDKTVLQSDDVWLVEFFAPWCGHCKNLEPEWAAAATAVKEQTKGKVRLGAVDATVHQVLSSRYGVRGFPTIKIFRKGEEPEDYQGGRTRGDIIERALDLFSD 272 HSP 2 Score: 95.5153 bits (236), Expect = 1.470e-20 Identity = 66/235 (28.09%), Postives = 110/235 (46.81%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLI-QDGNKVFKYEGDNSKEALIKFVHNPTQP---------------PPQKDVPWSEETNEIDFLTDANFYEYLDQHESV-IVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSE-GITGFPIFRYFNYGKNTSNSEYSLEREEDDFVA-----FMTNPANPQL 639
FYAPWCG+C+ + P + AAT LKG ++ D + L + + G+P++ + + NK +Y+G S +A++ N + Q D S + ++ LTD NF + + Q + V +V F+APWCGHCK +P++ +AA + E K + + D + + +S G+ GFP + F G+ + Y R D + F N P+L
Sbjct: 50 FYAPWCGHCRNLAPDWKKAATALKGIVKVGAVDADEHKS--LGGQYGVRGFPTIKIFGANKNKPEEYQGARSSQAIVDGAMNALRSLVKDRLSGKSGSSGYSRQSDSGSSGSSKDVVELTDDNFDKTVLQSDDVWLVEFFAPWCGHCKNLEPEWAAAATAVKEQTK-GKVRLGAVDATVHQVLSSRYGVRGFPTIKIFRKGEEPED--YQGGRTRGDIIERALDLFSDNAPPPEL 279
BLAST of Protein disulfide-isomerase A5 vs. Planmine SMEST
Match: SMESG000005268.1 (SMESG000005268.1) HSP 1 Score: 575.089 bits (1481), Expect = 0.000e+0 Identity = 277/359 (77.16%), Postives = 302/359 (84.12%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGING-DLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYE-GDNSKEALIKFVHNPTQPP-PQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKKKADA 1068
MFYAPWCG+CK MKP AA L+ I I D + ++ QAF I G+P+V + N F Y+ + S + L+KF+ +P +P P+ ++PW EE +++ F L +++ +VMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAM PSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKKKADA
Sbjct: 284 MFYAPWCGHCKAMKPAMVDAAVQLRKLKIDAQIAAIDSTENKNISQAFQIKGFPTVKYFK--NAQFMYDVNERSADGLVKFLLDPKKPEEPKLELPWKEEQSQVIHPVPEEFRAVLKRYKHALVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMMPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKKKADA 640 HSP 2 Score: 370.548 bits (950), Expect = 1.913e-119 Identity = 198/336 (58.93%), Postives = 225/336 (66.96%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFI-GVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP-QLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTET--HLAAQYKVSGFPTLVYFS--PNGDWVKYMGERTDDAITEFM 990
MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKA KP AA Q+ + K A I +D T+ +I + I GFP +YF KN ER D V F+ +P P + W+ ++ VI + ++ ALVMFY+PWCGHC A KP F +AAN++ I VD T E+ + ++GFP YF+ N +Y ER +D FM
Sbjct: 163 MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKAMKPAMVDAAVQLRKLKIDAQIAAIDSTENK--NISQAFQIKGFPTVKYF---KNAQFMYDVNERSADGLVKFLLDPKKPEEPKLELPWKE---EQSQVIHPVPEEFRAVLKRYKHALVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFI-GVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFM 489 HSP 3 Score: 137.502 bits (345), Expect = 8.483e-34 Identity = 83/275 (30.18%), Postives = 140/275 (50.91%), Query Frame = 1
Query: 199 YEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFL----------TDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMI 993
Y+ E++++F+ +PT ++ W E+ D + +NF + S+++MFYAPWCG+CK KP+F +AA + K FIG++ + D+ + ITG+P G +Y + ++ + F+ NP P W I L NF + + H S +VMFY+PWCGHC AMKP+ V+AA +L+ L IAA+D+T +++ +++ GFPT+ YF N ++ + ER+ D + +F++
Sbjct: 106 YDRKFRAESILRFLEDPTG-----EIAWEEDETARDIVHVKGSQDLQRLVSNFKTNI---PSMMLMFYAPWCGYCKQMKPQFAAAATTL--KGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKV--FKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNE--IDFLTDANFYEYLDQHES-VIVMFYAPWCGHCKAMKPAMVDAAVQLRKLKIDAQIAAIDSTENKNISQAFQIKGFPTVKYFK-NAQFMYDVNERSADGLVKFLL 364
BLAST of Protein disulfide-isomerase A5 vs. Planmine SMEST
Match: SMESG000077361.1 (SMESG000077361.1) HSP 1 Score: 170.244 bits (430), Expect = 7.689e-47 Identity = 101/344 (29.36%), Postives = 164/344 (47.67%), Query Frame = 1
Query: 1 MFYAPWCGYCKQMKPQFAAAATTL---KGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNP----------TQPPPQK-------DVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEK--------------NNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAV--DATTETHLAAQYKVSGFPTLVYF 924
MFYAPWC +C+ KP+ AAT + K++F +N + D+ Q NI GYP++ KV Y G ++E+L+KF+ NP ++ P DVP+ N +L NF+E L + + +V FYAPWC HC+ +KP A+ ++ N F V+C D +C I GFP F+Y++ G+ +EY E + ++ +++ P + + IP +A ++K F+ I + + ++M+Y+PWC HC KP F AA + P+ ++AAV D L + V+GFPT +++
Sbjct: 57 MFYAPWCPHCQDAKPELFKAATKMAIINSKNVFALLNCEDPKNTDICQQHNIEGYPTIKAFSPDKKVHVYNGPATEESLLKFIENPAMFSKSQDSASESPDSSADDENLPDVPF----NHTKYLNGENFHETLSKMKHALVFFYAPWCPHCQESKPSVDKASEKLKSNDHEIF-AVNCEDSKSKTLCVDNKIEGFPTFKYYHKGE--LFAEYEEELTAEAYLLYLSKP------PKEKVKEIPKEKASDEIQKPEEKMEFVKYLTSEETFLHEIKTPSRHTMIMYYAPWCSHCQTAKPIFNLAAKDISKSNPNHVLAAVNCDDKATQKLCEKEDVNGFPTFIHY 387 HSP 2 Score: 106.301 bits (264), Expect = 2.451e-24 Identity = 77/262 (29.39%), Postives = 114/262 (43.51%), Query Frame = 1
Query: 310 LTDANFYEYLDQHE-SVIVMFYAPWCGHCKATKPKFTSAANQMA-ENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNP---------ANPQLDARRHWRRIPG--HEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKK 1056
LT F Y + + + +VMFYAPWC HC+ KP+ AA +MA N K F ++C D DIC I G+P + F+ K Y+ E+ + F+ NP A+ D+ +P L NF +S ALV FY+PWC HC KPS A+ KL++ + + L K+ GFPT Y+ + +Y E T +A ++ K + ++ EK E +K
Sbjct: 38 LTSEFFENYTTKSDGTTVVMFYAPWCPHCQDAKPELFKAATKMAIINSKNVFALLNCEDPKNTDICQQHNIEGYPTIKAFSPDKKVHV--YNGPATEESLLKFIENPAMFSKSQDSASESPDSSADDENLPDVPFNHTKYLNGENF-HETLSKMKHALVFFYAPWCPHCQESKPSVDKASEKLKSNDHEIFAVNCEDSKSKTLCVDNKIEGFPTFKYYHKGELFAEYEEELTAEAYLLYLSKPPKEKVKEIPKEKASDEIQK 296 HSP 3 Score: 97.0561 bits (240), Expect = 2.256e-21 Identity = 59/200 (29.50%), Postives = 99/200 (49.50%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFI-GINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQK--DVPWSEETNEIDFLTDA-NFYEYLDQHES-----------VIVMFYAPWCGHCKATKPKFTSAANQMAE-NKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGK 555
FYAPWC +C++ KP A+ LK I +N + S + L I G+P+ G +YE + + EA + ++ ++PP +K ++P + ++EI + F +YL E+ ++M+YAPWC HC+ KP F AA +++ N V+C D++ +C E + GFP F ++ GK
Sbjct: 195 FYAPWCPHCQESKPSVDKASEKLKSNDHEIFAVNCEDSKSKTLCVDNKIEGFPTFKYYHKGELFAEYEEELTAEAYLLYL---SKPPKEKVKEIPKEKASDEIQKPEEKMEFVKYLTSEETFLHEIKTPSRHTMIMYYAPWCSHCQTAKPIFNLAAKDISKSNPNHVLAAVNCDDKATQKLCEKEDVNGFPTFIHYFEGK 391
BLAST of Protein disulfide-isomerase A5 vs. Planmine SMEST
Match: SMESG000010656.1 (SMESG000010656.1) HSP 1 Score: 163.696 bits (413), Expect = 5.593e-43 Identity = 92/234 (39.32%), Postives = 126/234 (53.85%), Query Frame = 1
Query: 310 LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIG-VDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKKHSD 1008
L NF E+++ + V+V FYAPWCGHCK P +T AA Q+ +N +G VD T ES ++ T GI+G+P ++F G +Y R E V FM +P W+ P ++ L K NF Q IV + LV F++PWCGHC ++ P F AA L L P +A VDAT ET LA +Y VSG+P+L F +V Y G R+ D I ++MIK +
Sbjct: 33 LNSENFDEFINANNIVMVEFYAPWCGHCKQLTPIYTKAAVQLKKNNPPILLGKVDATVES--ELATRFGISGYPTLKFFKSG---IPYDYDDSRSESAIVNFMKKVGSPD------WQ--PPKNYVLTLTKENFTQ-IVESSGYILVEFFAPWCGHCKSLAPEFAKAAKILSNLNPPIQLATVDATVETDLAKEYHVSGYPSLKLFKNKNFFVDYSGPRSSDGIVDWMIKNREN 252 HSP 2 Score: 109.768 bits (273), Expect = 6.475e-25 Identity = 67/207 (32.37%), Postives = 104/207 (50.24%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKS--IFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPPPQKDVPWSEETNEIDFLTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAE-NKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFM 615
FYAPWCG+CKQ+ P + AA LK + I +G D + +L F I+GYP++ + G + Y+ S+ A++ F+ P W N + LT NF + ++ ++V F+APWCGHCK+ P+F AA ++ N + VD T E+ D+ ++G+P + F KN +YS R D V +M
Sbjct: 52 FYAPWCGHCKQLTPIYTKAAVQLKKNNPPILLG-KVDATVESELATRFGISGYPTLKFFKSGIP-YDYDDSRSESAIVNFMKKVGSPD------WQPPKNYVLTLTKENFTQIVESSGYILVEFFAPWCGHCKSLAPEFAKAAKILSNLNPPIQLATVDATVET--DLAKEYHVSGYPSLKLFK-NKNFF-VDYSGPRSSDGIVDWM 246
BLAST of Protein disulfide-isomerase A5 vs. Planmine SMEST
Match: SMESG000017020.1 (SMESG000017020.1) HSP 1 Score: 107.842 bits (268), Expect = 7.929e-25 Identity = 78/272 (28.68%), Postives = 126/272 (46.32%), Query Frame = 1
Query: 310 LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQLDARRHWR------RIPGHEA------------IVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSP---NGDWVKYMGERTDDAITEFMIKKHSDIIRHEEAEKLFQEEKKKA 1062
LT +NF + + V FYAPWCGHC++ P++ AA + KV + +D ++ + GI GFP + F K+ S +Y +R V N A +LD R HE +V L +NF + ++ LV F++PWCGHC +KP + AA++L+ + + A+DAT T A++Y V G+PT+ +F +GD Y G R+ I + K + + E +++ +E K+
Sbjct: 29 LTPSNFDDMKNSDSLWFVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMD----KYSSLGQPYGIQGFPTIKIFGKSKH-SPKDYQGDRTAGSIV----NAALKELDELVRSRLGGASGGSSSHERKSSGGSSGSGGDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKNLKPHWDQAASELRGKVK---LGALDATVHTATASRYGVQGYPTIKFFPAGVKSGDSQDYDGGRSSSDIVRWASDKVVEQLPAPEIKEILNQEILKS 288 HSP 2 Score: 94.7449 bits (234), Expect = 1.542e-20 Identity = 66/233 (28.33%), Postives = 108/233 (46.35%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFK-YEGDNSKEALIKFVHNPTQPPPQKDV----------------PWSEETNEIDFLTDANFYEY-LDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSE-YSLEREEDDFVAFMTNPANPQLDA 645
FYAPWCG+C+ + P++ AAT LKG + +G D+ L Q + I G+P++ + K Y+GD + +++ + + S ++ LTD+NF E L E +V F+APWCGHCK KP + AA+++ KV +D T + + G+ G+P ++F G + +S+ Y R D V + ++ QL A
Sbjct: 48 FYAPWCGHCQSLAPEWKKAATALKG-VVKVGAV-DMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGDRTAGSIVNAALKELDELVRSRLGGASGGSSSHERKSSGGSSGSGGDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKNLKPHWDQAASEL--RGKVKLGALDATVHTAT--ASRYGVQGYPTIKFFPAGVKSGDSQDYDGGRSSSDIVRWASDKVVEQLPA 274
BLAST of Protein disulfide-isomerase A5 vs. Planmine SMEST
Match: SMESG000023147.1 (SMESG000023147.1) HSP 1 Score: 89.7373 bits (221), Expect = 9.656e-19 Identity = 44/112 (39.29%), Postives = 66/112 (58.93%), Query Frame = 1
Query: 664 IPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGDWVKYMGERTDDAITEFMIKK 999
I E +++L +NF ++ LV FY+PWCGHC A+ P + +AA KL+ +A VDAT E LA+++K+ G+PTL +F +G + Y G RT D I ++ KK
Sbjct: 20 ITEEEGVLVLTNSNF-NEVIEKNKYVLVEFYAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFK-DGKVMDYGGGRTADTIISWLEKK 129 HSP 2 Score: 76.2554 bits (186), Expect = 2.047e-14 Identity = 39/109 (35.78%), Postives = 63/109 (57.80%), Query Frame = 1
Query: 310 LTDANFYEYLDQHESVIVMFYAPWCGHCKATKPKFTSAANQMAE-NKKVAFIGVDCTDESFADICTSEGITGFPIFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANP 633
LT++NF E +++++ V+V FYAPWCGHCKA P++ SAA ++ E N + VD T E+ D+ + I G+P ++F GK +Y R D ++++ P
Sbjct: 29 LTNSNFNEVIEKNKYVLVEFYAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVEN--DLASKFKIQGYPTLKFFKDGKVM---DYGGGRTADTIISWLEKKTGP 132 HSP 3 Score: 74.3294 bits (181), Expect = 1.006e-13 Identity = 35/87 (40.23%), Postives = 52/87 (59.77%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGI-NGDLSTTDDLKQAFNITGYPSVFLIQDGNKVFKYEGDNSKEALIKFVHNPTQPP 261
FYAPWCG+CK + P++A+AA LK K+ I + D + +DL F I GYP++ +DG KV Y G + + +I ++ T PP
Sbjct: 48 FYAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG-KVMDYGGGRTADTIISWLEKKTGPP 133 HSP 4 Score: 68.9366 bits (167), Expect = 4.905e-12 Identity = 51/181 (28.18%), Postives = 82/181 (45.30%), Query Frame = 1
Query: 34 QMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNI--TGYPSVFLIQDGNKVFKYEGDN---SKEALIKFVHNPTQPPPQ-----KDVPWSEETNEIDFLTDANFYEYL-DQHESVIVMFYAPWCGHCKATKPKFTSAANQMAENKKVAFIGVDCTDESFADICTSEGITGFPIFRYF 543
++K QF +A G+ +F+ IN D D + + F + T PS +IQ + + K++ D+ S E L FV + + +++P + N + L NF D+ ++V V FYAPWCGHCK P + +N + +D T A+ I FP +YF
Sbjct: 268 ELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDST----ANEVEGVRIQSFPTIKYF 444 HSP 5 Score: 49.6766 bits (117), Expect = 5.101e-6 Identity = 25/82 (30.49%), Postives = 46/82 (56.10%), Query Frame = 1
Query: 4 FYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITGYPSV-FLIQDGNKVFKYEGDNSKEALIKFVHN 246
FYAPWCG+CKQ+ P + T K + + D ST +++ + I +P++ + +D +++ +EGD + E KF+ +
Sbjct: 390 FYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMD-STANEV-EGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDS 469 HSP 6 Score: 49.2914 bits (116), Expect = 6.119e-6 Identity = 41/177 (23.16%), Postives = 69/177 (38.98%), Query Frame = 1
Query: 529 IFRYFNYGKNTSNSEYSLEREEDDFVAFMTNPANPQ-----------LDAR-----------RHWRRIPGHEAIVILEKNNFVQNIVSHTSGALVMFYSPWCGHCIAMKPSFVNAANKLQTLLPSCLIAAVDATTETHLAAQYKVSGFPTLVYFSPNGD-WVKYMGERTDDAITEFM 990
+ +F K T + + + EDD V F + A LD + W + P + +L NF + V FY+PWCGHC + P + + +IA +D+T + ++ FPT+ YF + D V + G+RT + +F+
Sbjct: 299 VLEFFGMAK-TDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNP----VKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKD-NTDIVIAKMDST--ANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFI 467 The following BLAST results are available for this feature:
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 4
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Protein disulfide-isomerase A5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035571 ID=SMED30035571|Name=Protein disulfide-isomerase A5|organism=Schmidtea mediterranea sexual|type=transcript|length=1773bpback to top protein sequence of SMED30035571-orf-1 >SMED30035571-orf-1 ID=SMED30035571-orf-1|Name=SMED30035571-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=558bp MFYAPWCGYCKQMKPQFAAAATTLKGKSIFIGINGDLSTTDDLKQAFNITback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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