SMED30035524

Overview
NameSMED30035524
Smed IDSMED30035524
Length (bp)459
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30035524 (SMED30035524) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30035524 (SMED30035524) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035524

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30035524 SmedASXL_080301SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30035524 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001408:64642..75090 -2146
v31.004619:809..11449 -1938
Homology
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BLAST of SMED30035524 vs. Planmine SMEST
Match: SMESG000002321.1 (SMESG000002321.1)

HSP 1 Score: 67.781 bits (164), Expect = 1.365e-14
Identity = 31/50 (62.00%), Postives = 42/50 (84.00%), Query Frame = 1 
Query:  100 MESNKRLIEKFRRDISKYNREALNQNQRAKAMV--LNNMQQTWLRPKTKT 243
            MESN+R+IEKF RDIS+Y+ EALNQ+QRA+AM+    +M+QTWLRP+  +
Sbjct:    1 MESNRRIIEKFCRDISEYHSEALNQSQRAQAMIRKCRHMEQTWLRPEAAS 50
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BLAST of SMED30035524 vs. Planmine SMEST
Match: SMESG000019606.1 (SMESG000019606.1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.712e-14
Identity = 31/48 (64.58%), Postives = 41/48 (85.42%), Query Frame = 1 
Query:  100 MESNKRLIEKFRRDISKYNREALNQNQRAKAMV--LNNMQQTWLRPKT 237
            MESN+R+IEKFR DIS+Y+ +ALNQ+QRA+AMV    +M+QTWLRP+ 
Sbjct:    4 MESNRRIIEKFRMDISEYHWKALNQSQRAEAMVRKCRHMEQTWLRPEA 51
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BLAST of SMED30035524 vs. Planmine SMEST
Match: SMESG000052813.1 (SMESG000052813.1)

HSP 1 Score: 59.6918 bits (143), Expect = 7.090e-12
Identity = 30/60 (50.00%), Postives = 44/60 (73.33%), Query Frame = 1 
Query:  100 MESNKRLIEKFRRDISKYNREALNQNQRAKAMV--LNNMQQTWLRPKTKTSKLTDAPPRV 273
            +ESN+RLIEKFRRD+++Y+ EA+ Q+QRA+ M     +++QTWLRP+   SKL     R+
Sbjct:   43 VESNRRLIEKFRRDVAEYHHEAMIQSQRAETMARKCRHLEQTWLRPEA-VSKLKSEIERL 101
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BLAST of SMED30035524 vs. Planmine SMEST
Match: SMESG000007350.1 (SMESG000007350.1)

HSP 1 Score: 54.6842 bits (130), Expect = 7.004e-10
Identity = 24/47 (51.06%), Postives = 39/47 (82.98%), Query Frame = 1 
Query:  100 MESNKRLIEKFRRDISKYNREALNQNQRAKAMV--LNNMQQTWLRPK 234
            +ESN+++IEKFR+D ++Y+ EA+ QNQR +A+V    +++QTWLRP+
Sbjct:   33 VESNRQMIEKFRKDHAQYHHEAMVQNQRPEALVRKCRHLEQTWLRPE 79
[Close]
BLAST of SMED30035524 vs. Planmine SMEST
Match: SMESG000001257.1 (SMESG000001257.1)

HSP 1 Score: 55.4546 bits (132), Expect = 2.090e-9
Identity = 24/47 (51.06%), Postives = 37/47 (78.72%), Query Frame = 1 
Query:  100 MESNKRLIEKFRRDISKYNREALNQNQRAKAMV--LNNMQQTWLRPK 234
            ME N ++IE+FR+D++KY++EA+ QNQRA A+V     ++QTWL P+
Sbjct:  665 MEQNNKIIERFRKDVAKYHQEAMVQNQRAVALVRRCRRIEQTWLGPE 711
The following BLAST results are available for this feature:
BLAST of SMED30035524 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035524 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000002321.11.365e-1462.00SMESG000002321.1[more]
SMESG000019606.13.712e-1464.58SMESG000019606.1[more]
SMESG000052813.17.090e-1250.00SMESG000052813.1[more]
SMESG000007350.17.004e-1051.06SMESG000007350.1[more]
SMESG000001257.12.090e-951.06SMESG000001257.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035524 ID=SMED30035524|Name=SMED30035524|organism=Schmidtea mediterranea sexual|type=transcript|length=459bp
ATGGATGCTGAATTTTTTAGTTTTATCCCCCAGTTATCGAAGTGGAATTC
TCTAACAACCAAATGCTTGCCTGCGTTTGCCGCTTATTCATTATCTCAAA
TGGAGTCGAATAAAAGATTAATAGAGAAATTCCGAAGGGATATTTCAAAG
TACAATCGGGAGGCGCTAAATCAAAATCAGAGAGCAAAGGCCATGGTGCT
GAACAACATGCAACAAACCTGGCTCCGTCCAAAAACAAAGACGAGTAAGT
TAACTGATGCCCCCCCTCGTGTAATTTTCAAATCATTAAAAAATGAAAAA
AATTATCGATATATTCCTGAGCTTGTGAATTTTGCATCTCGTAGAGAATG
TCCAGAAGCCTGGTCCGACGAAGAGAGGGTTAATTATAAAATGTTGGGTG
GATTTGTTATGTGGCGAAGATTTTGGAAGATGGGAAAAGTGTTAATAGGA
GATCGTTGA
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protein sequence of SMED30035524-orf-1

>SMED30035524-orf-1 ID=SMED30035524-orf-1|Name=SMED30035524-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=153bp
MDAEFFSFIPQLSKWNSLTTKCLPAFAAYSLSQMESNKRLIEKFRRDISK
YNREALNQNQRAKAMVLNNMQQTWLRPKTKTSKLTDAPPRVIFKSLKNEK
NYRYIPELVNFASRRECPEAWSDEERVNYKMLGGFVMWRRFWKMGKVLIG
DR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell