Ast7 protein

Overview
NameAst7 protein (CCW36845.1)
Smed IDSMED30035521
Length (bp)809
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ast7 protein (SMED30035521) t-SNE clustered cells

Violin plots show distribution of expression levels for Ast7 protein (SMED30035521) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035521

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30035521h1SMcG0003742 SmedASXL_004498SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30035521h1SMcG0003742 SmedASXL_004498SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30035521h1SMcG0003742 OX_Smed_1.0.20209ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ast7 protein vs. Ensembl Human
Match: ASTL (astacin like metalloendopeptidase [Source:HGNC Symbol;Acc:HGNC:31704])

HSP 1 Score: 86.6557 bits (213), Expect = 8.323e-19
Identity = 62/218 (28.44%), Postives = 102/218 (46.79%), Query Frame = 3 
Query:  159 GDMV-LSPVEILKINGYKAADGEKWPKN-----ELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKF---KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRN--VKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGCA 773
            GD++  SP  +L      +A   KWP       E+ +     + + ++  + + L  +E+    CI+F   ++++   F +++   GC ++VG +    + V+LA  C+ K    V HE  H LGF HEHTR DRD  + + +N +      NF   +     TPYD+ S+M Y   AF+  G  TI  L      I     ++  D  +V   YGC+
Sbjct:   76 GDIIRPSPFRLL------SATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERS--TCIRFVTYQDQRD--FISIIPMYGCFSSVGRSGGMQV-VSLAPTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSQSSNMLTPYDYSSVMHYGRLAFSRRGLPTITPLWAPSVHIGQRWNLSASDITRVLKLYGCS 282
[Close]
BLAST of Ast7 protein vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 77.7962 bits (190), Expect = 8.552e-16
Identity = 58/192 (30.21%), Postives = 83/192 (43.23%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVT--GGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTT---PYDFDSIMQYPSEAFTCNG-KETIL--RKNGG---KIAFNMEVTKLDAKKVRDYYGC 770
            WP   + Y    +F    +   +  ++ +EK    C+ F E        V T    GC + VG     P  +++   C     V HE  H +GF HEHTRPDRDN++ I   N++     NF   E  +  +    YDFDSIM Y    F+     +TIL  R + G    I     ++K D  + R  Y C
Sbjct:  157 WPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of Ast7 protein vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 76.6406 bits (187), Expect = 3.390e-15
Identity = 58/192 (30.21%), Postives = 83/192 (43.23%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVT--GGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTT---PYDFDSIMQYPSEAFTCNG-KETIL--RKNGG---KIAFNMEVTKLDAKKVRDYYGC 770
            WP   + Y    +F    +   +  ++ +EK    C+ F E        V T    GC + VG     P  +++   C     V HE  H +GF HEHTRPDRDN++ I   N++     NF   E  +  +    YDFDSIM Y    F+     +TIL  R + G    I     ++K D  + R  Y C
Sbjct:  157 WPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of Ast7 protein vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 76.6406 bits (187), Expect = 3.470e-15
Identity = 58/192 (30.21%), Postives = 83/192 (43.23%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVT--GGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTT---PYDFDSIMQYPSEAFTCNG-KETIL--RKNGG---KIAFNMEVTKLDAKKVRDYYGC 770
            WP   + Y    +F    +   +  ++ +EK    C+ F E        V T    GC + VG     P  +++   C     V HE  H +GF HEHTRPDRDN++ I   N++     NF   E  +  +    YDFDSIM Y    F+     +TIL  R + G    I     ++K D  + R  Y C
Sbjct:  157 WPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of Ast7 protein vs. Ensembl Human
Match: TLL2 (tolloid like 2 [Source:HGNC Symbol;Acc:HGNC:11844])

HSP 1 Score: 76.2554 bits (186), Expect = 4.595e-15
Identity = 58/193 (30.05%), Postives = 89/193 (46.11%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKE---EKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTT---PYDFDSIMQYPSEAFTCNG-KETILRK---NGGK--IAFNMEVTKLDAKKVRDYYGC 770
            WP   + Y    +F    +   +  ++ +EK    C+ F E   E+S +  +  T G CS  VG     P  +++   C     V HE  H +GF HEHTRPDRD ++ I   N++     NF   E  + ++    YDFDSIM Y    F+     +TIL +   NG +  I   + +++ D  + R  Y C
Sbjct:  159 WPGGVIPYVIGGNFTGSQRAIFKQAMRHWEKH--TCVTFIERTDEESFIVFSYRTCGCCSY-VGRRGGGPQAISIGKNCDKFGIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFSRGVFLDTILPRQDDNGVRPTIGQRVRLSQGDIAQARKLYKC 348
[Close]
BLAST of Ast7 protein vs. Ensembl Celegans
Match: nas-14 (Zinc metalloproteinase nas-14 [Source:UniProtKB/Swiss-Prot;Acc:Q19269])

HSP 1 Score: 87.4261 bits (215), Expect = 2.309e-19
Identity = 54/190 (28.42%), Postives = 93/190 (48.95%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVT----GGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANF--YLCEGCKTT-TPYDFDSIMQYPSEAFTCNGKETILRK-NGGKIAFNMEVTKLDAKKVRDYYGCA 773
            WP+ ++ Y  +    +  +  +      Y+ +   C++F  +    F  +        GCS+ VG            D C +K  + HE  H+LGF HEH+R DRD+ ++I  +N++  +  NF  Y  +   +   PYD++S+M Y   AF+ NGK TI+ K N   +    +++++D+KKV   Y C 
Sbjct:  125 WPEGQVPYMLEEGMTNDQRTAIAQAFDEYKTK--TCVRFVPKTDDDFDYIYVKRNVAFGCSSYVGRAGGNQTVSLEVDKCFSKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNIRPGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFSRNGKPTIIPKDNEADVGQRYKLSEMDSKKVNKLYQCG 312
[Close]
BLAST of Ast7 protein vs. Ensembl Celegans
Match: nas-6 (Zinc metalloproteinase nas-6 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3S9])

HSP 1 Score: 86.2705 bits (212), Expect = 2.419e-19
Identity = 57/189 (30.16%), Postives = 89/189 (47.09%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEK-ELGNCIKF-KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTP---YDFDSIMQYPSEAFTCNGKETILRKNGG---KIAFNMEVTKLDAKKVRDYYGC 770
            W    + Y  D +F   + N ++   KA++      CI+F K E    +  +V G GC + VG       +++L   C     + HE  HS+GF HEH+R DRD+++ I ++N+   + + F              YD+ SIM Y S AF+ NG+ TI     G    I   M+++ LD  K+   Y C
Sbjct:   81 WEGGVIPYEMDTAF---SPNEIKILEKAFDSYRRTTCIRFEKREGQTDYLNIVKGYGCYSQVGRTGG-KQEISLGRGCFFHEIIVHELMHSVGFWHEHSRADRDDHIKINWDNILPGMKSQFDKISAVLQDLQGENYDYKSIMHYDSTAFSRNGRNTIETVENGFTQVIGTAMDLSPLDIVKINKLYSC 265
[Close]
BLAST of Ast7 protein vs. Ensembl Celegans
Match: nas-7 (Zinc metalloproteinase nas-7 [Source:UniProtKB/Swiss-Prot;Acc:P55113])

HSP 1 Score: 86.6557 bits (213), Expect = 2.565e-19
Identity = 59/199 (29.65%), Postives = 94/199 (47.24%), Query Frame = 3 
Query:  192 KINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTG--GGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTT---TPYDFDSIMQYPSEAFTCNGKETILRK-NGGKIAFNMEVTKLDAKKVRDYYGC 770
            K NG   A  + WP   + Y   P +    +  +   +K Y ++   CI+F   ++G    +  G   GC + VG  S   + ++L + C+    + HE  H +GF HEH R DRDN ++I + N+ +     F   +  KT+    PYD+ SI+ Y S AF+ NG  T+L K     I    + + +D  K+   Y C
Sbjct:   78 KRNGVSRA-AKLWPNARIPYAISPHYSPHERALLAKAVKQYHEK--TCIRFVPRQTGEPDYLFIGKVDGCFSEVGRTSGVQV-LSLDNGCMEYATIIHEMMHVVGFYHEHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFSKNGFPTMLPKVKSATIGNARDFSDVDISKINRMYNC 272
[Close]
BLAST of Ast7 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 83.9593 bits (206), Expect = 1.308e-18
Identity = 65/240 (27.08%), Postives = 114/240 (47.50%), Query Frame = 3 
Query:  132 GIKIAD------FNVGDMVL-SPVEILKINGYKAADG-----EKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKF---KEEKSGVFATVVTGGGCSANVG-YNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNV---------KKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGG--KIAFNMEVTKLDAKKVRDYYGC 770
            GIK+ D      ++ GD++L SP + ++ N     +       +WP NE+ YT    +    ++ + + +  Y  +   C+KF      K   +  +    GC + VG    + P  V+L   C+    + HE  H++GF HE +R DRD+ +++ + NV         K NL    +L E      PYD+ SIM Y   AF+ +GK+T++ K  G  ++   ++ + +D +K+   Y C
Sbjct:   60 GIKVKDDPTIGNYSEGDILLESPKKFVEENNKLGRNAIKQIYRRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHPDEGCYSLVGKTGGKQP--VSLDSGCIQVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDE------PYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast7 protein vs. Ensembl Celegans
Match: nas-12 (Zinc metalloproteinase nas-12 [Source:UniProtKB/Swiss-Prot;Acc:Q9XTD6])

HSP 1 Score: 84.7297 bits (208), Expect = 1.379e-18
Identity = 63/221 (28.51%), Postives = 98/221 (44.34%), Query Frame = 3 
Query:  159 GDMVLSPVEILKINGYKAAD------------GEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKS-GVFATVVTGGGCSANVG-YNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTP--YDFDSIMQYPSEAFTCNGKETILR-------KNGGKIAFNMEVTKLDAKKV 752
            GDM+L+P ++++    K +D              +W  N + Y   P +    K  +   L+ +E+   +C KF E  +   +  +V   GC + VG    R  L  +LA  C+    + HE  H++GF+HEH RPDRD+ + + + NV      NF   +      P  YDF SIM Y   AF        +R       K G  +  NM+ T  D +K+
Sbjct:   48 GDMLLTPAQLIRYENSKDSDLSIRGVSIKGSSMNRWSNNIVPYVISPQYSPAQKQILVSSLRYFERV--SCFKFVERTTQNDYLFIVPLDGCYSYVGKIGGRQTL--SLAADCIADYIIWHEMMHAIGFEHEHQRPDRDSFIRVDYANVIPGQMINFDKLKTSHVEYPDIYDFKSIMHYDGYAFGRVDTARRVRLATMTPLKPGVTLEDNMKFTATDIEKL 264
[Close]
BLAST of Ast7 protein vs. Ensembl Fly
Match: CG11865 (gene:FBgn0028947 transcript:FBtr0080753)

HSP 1 Score: 88.1965 bits (217), Expect = 1.368e-20
Identity = 55/186 (29.57%), Postives = 86/186 (46.24%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVT---GGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTT---PYDFDSIMQYPSEAFTCNGKETIL-RKNGGKIAFNMEVTKLDAKKVRDYY 764
            W    L Y++   F   +   +   +   E     C+KF+  +      VV    G GC + VGY  R    + L   C++ R ++HE  H+LGF H H+ P RD  + IQ +N++     NF        T     YD+DSIM Y   AF+ NG+ TI+  K+  KI    +++  D + ++  Y
Sbjct:   56 WKGRTLVYSYAGGFSSLDIASIESAMA--EISSKTCVKFRRTEYKREPQVVIQKEGSGCWSYVGYLGRADQTLNLGSGCMSNRTIQHELLHALGFFHTHSDPQRDKYVRIQTDNIRSGHEHNFQRLRANGVTNYGFGYDYDSIMHYGPFAFSKNGQSTIVPLKSHAKIGQATQMSPKDVQTLKRMY 239
[Close]
BLAST of Ast7 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0084378)

HSP 1 Score: 83.9593 bits (206), Expect = 2.252e-18
Identity = 61/213 (28.64%), Postives = 100/213 (46.95%), Query Frame = 3 
Query:  159 GDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTG--GGCSANVGYNSRYPLKVTLADP-CVNKRNVK-HEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKT-TTPYDFDSIMQYPSEAFTCNGKETIL--RKNGG-KIAFNMEVTKLDAKKVRDYYGCA 773
            GDM++   +++  NG       +WP   + Y    +F  ++   + + +  Y +    CI+F +  S      + G   GC ++VG       +V L  P C+++     HE  H+LGF HE  R +RD  + IQ+NNV+ +   NF      +    PYD+ S+M Y   AF+ NG+ TIL  + NG  K+      +  D +K+   Y C 
Sbjct:   95 GDMLVPQTDLIMKNGLPT-QSSRWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVGRVGGKQ-EVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNGFSDYDIQKLNRMYDCG 303
[Close]
BLAST of Ast7 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0334497)

HSP 1 Score: 83.9593 bits (206), Expect = 2.252e-18
Identity = 61/213 (28.64%), Postives = 100/213 (46.95%), Query Frame = 3 
Query:  159 GDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTG--GGCSANVGYNSRYPLKVTLADP-CVNKRNVK-HEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKT-TTPYDFDSIMQYPSEAFTCNGKETIL--RKNGG-KIAFNMEVTKLDAKKVRDYYGCA 773
            GDM++   +++  NG       +WP   + Y    +F  ++   + + +  Y +    CI+F +  S      + G   GC ++VG       +V L  P C+++     HE  H+LGF HE  R +RD  + IQ+NNV+ +   NF      +    PYD+ S+M Y   AF+ NG+ TIL  + NG  K+      +  D +K+   Y C 
Sbjct:   95 GDMLVPQTDLIMKNGLPT-QSSRWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVGRVGGKQ-EVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNGFSDYDIQKLNRMYDCG 303
[Close]
BLAST of Ast7 protein vs. Ensembl Fly
Match: CG6974 (gene:FBgn0038285 transcript:FBtr0083042)

HSP 1 Score: 78.9518 bits (193), Expect = 3.935e-17
Identity = 53/199 (26.63%), Postives = 92/199 (46.23%), Query Frame = 3 
Query:  222 EKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVV------TGGGCSANVGYN--SRYPLKVTLADPCVNKRNV-KHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKT-TTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKL------DAKKVRDYYGC 770
            ++WP N++ Y     + ++    VR  + ++ ++   C++F+E +    A         +   C   VGY   S  P +V L + C+    V +HE  H LG  HE +RPDRD  + I ++N+ +   + F   +   T   PYD++S+M Y   AF  +  +  +R   G  A   E+ ++      D  K+R  Y C
Sbjct:   55 QRWPGNKILYRISTDYSEQEVANVRTAMSSFGEQ--TCVQFEEIEGAPPAGKRYVSFKKSPNMCGTRVGYQPLSFGPHEVVLNEKCLTMPAVIQHETLHLLGLFHEQSRPDRDEYVQIDYDNIPRKYWSQFMAMDQTTTFNVPYDYESVMHYSKNAFAKDPSKPTIRALIGGKAVEREMGQVRGPSEGDWTKIRLMYKC 251
[Close]
BLAST of Ast7 protein vs. Ensembl Fly
Match: CG15255 (gene:FBgn0028950 transcript:FBtr0080785)

HSP 1 Score: 79.337 bits (194), Expect = 4.383e-17
Identity = 62/229 (27.07%), Postives = 98/229 (42.79%), Query Frame = 3 
Query:  150 FNVGDMVLSPVEI--LKINGYKAADG-----EKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKE---EKSGVFATVVTGGGCSANVGYNSR--------YPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTT---TPYDFDSIMQYPSEAFTCNGKETIL-RKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            F  GDM+L+  +   L+    KA +G     ++WP N + Y     F   +K  ++  +   E  L  C++F+E   E           GGC   VGY           YPL     + C     + HEF H+LGF H+ +   RD+ +N+ + N+      NF        T     YD+DS + Y   AF+ NG++TI+   +   I   + ++  D  K+   Y C
Sbjct:   35 FVEGDMMLTEEQQRNLEQGAPKARNGLINTEKRWPGNVVVYRISDDFDTAHKKAIQTGIDTLE--LHTCLRFREATDEDKAYLTVTAKSGGCYTAVGYQGAPQEMNLEIYPL----GEGCFRPGTILHEFMHALGFYHQQSSSIRDDFINVIYENIVPGKEFNFQKYADTVVTDFEVGYDYDSCLHYRPGAFSINGEDTIVPLDSSAVIGQRVGLSSKDIDKINIMYKC 257
[Close]
BLAST of Ast7 protein vs. Ensembl Zebrafish
Match: CU326366.2 (pep chromosome:GRCz11:10:42887204:42898220:-1 gene:ENSDARG00000068593.5 transcript:ENSDART00000099270.5 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CU326366.2)

HSP 1 Score: 84.3445 bits (207), Expect = 1.065e-18
Identity = 59/196 (30.10%), Postives = 92/196 (46.94%), Query Frame = 3 
Query:  207 KAADGEKWPKNE----LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGV-FATVVTGGGCSANVG-YNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETIL-RKNGGKIAFNMEVTKLDAKKVRDYYGCA 773
            + A    WP+ +    + Y+      DK  + +   LK   K+   C+KF    +   +        C++ VG      P+   L  P  N  N+ HE  HSLG  HEH+RPDRD  + I ++N+     +NF + +G      YD DSI+ Y  + F+ NG  TI+ +K G KI     ++ LD +++R  Y C 
Sbjct:   20 RNAGNHLWPEKDGEVSVPYSIASGLEDKTGH-ILAALKMVSKK--TCVKFHHHTTEEDYLHFKPDRMCASLVGCAGGEQPI---LVGPKCNAGNICHEILHSLGLYHEHSRPDRDKYITILYDNIMPGKESNFKVKKGNTLGLEYDLDSILHYGDDCFSRNGNHTIIPKKKGVKIGQRTHMSVLDVERLRRLYHCG 209
[Close]
BLAST of Ast7 protein vs. Ensembl Zebrafish
Match: c6ast3 (six-cysteine containing astacin protease 3 [Source:ZFIN;Acc:ZDB-GENE-050320-99])

HSP 1 Score: 82.8037 bits (203), Expect = 2.534e-18
Identity = 48/150 (32.00%), Postives = 77/150 (51.33%), Query Frame = 3 
Query:  333 CIKF-KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADP-CVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            CI+F        + ++ +  GC + VG    Y   V+LA   C+    V+HE  H+LGF HE TR DRDN++ + + N+  ++  NF         TPYD+ S+MQY   AF+ NG  T+  +  N   +  + E+++ D  ++   Y C
Sbjct:  107 CIRFFPRGNERDYISIESRSGCYSYVGRQG-YAQTVSLARSGCLYHSTVQHELLHALGFNHEQTRNDRDNHIQVIWENILDDMKYNFNKINTLNQGTPYDYSSVMQYERYAFSKNGLPTMIPIPNNNAALGTSTEMSQNDIIRINRLYQC 255
[Close]
BLAST of Ast7 protein vs. Ensembl Zebrafish
Match: c6ast3 (six-cysteine containing astacin protease 3 [Source:ZFIN;Acc:ZDB-GENE-050320-99])

HSP 1 Score: 82.8037 bits (203), Expect = 2.534e-18
Identity = 48/150 (32.00%), Postives = 77/150 (51.33%), Query Frame = 3 
Query:  333 CIKF-KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADP-CVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            CI+F        + ++ +  GC + VG    Y   V+LA   C+    V+HE  H+LGF HE TR DRDN++ + + N+  ++  NF         TPYD+ S+MQY   AF+ NG  T+  +  N   +  + E+++ D  ++   Y C
Sbjct:  107 CIRFFPRGNERDYISIESRSGCYSYVGRQG-YAQTVSLARSGCLYHSTVQHELLHALGFNHEQTRNDRDNHIQVIWENILDDMKYNFNKINTLNQGTPYDYSSVMQYERYAFSKNGLPTMIPIPNNNAALGTSTEMSQNDIIRINRLYQC 255
[Close]
BLAST of Ast7 protein vs. Ensembl Zebrafish
Match: c6ast4 (six-cysteine containing astacin protease 4 [Source:ZFIN;Acc:ZDB-GENE-050626-100])

HSP 1 Score: 82.0333 bits (201), Expect = 5.992e-18
Identity = 43/150 (28.67%), Postives = 75/150 (50.00%), Query Frame = 3 
Query:  330 NCIKF-KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            +CI+F +      + ++ +  GC + VG              C+    V+HE  H+LGF HE TR DRDN++ + + N+  ++  NF         TPYD+ S+MQY   AF+ NG  T+  +  N  ++  + ++++ D  ++   Y C
Sbjct:  116 SCIRFFRRSNERDYISIESRSGCYSYVGRQGNVQTVSLARSGCLYHSTVQHELLHALGFNHEQTRSDRDNHIQVIWENILDDMKYNFNKINTLNQGTPYDYKSVMQYERYAFSKNGYPTMIPIPNNNAELGKSTQMSQNDITRLNRLYQC 265
[Close]
BLAST of Ast7 protein vs. Ensembl Zebrafish
Match: c6ast4 (six-cysteine containing astacin protease 4 [Source:ZFIN;Acc:ZDB-GENE-050626-100])

HSP 1 Score: 82.0333 bits (201), Expect = 5.992e-18
Identity = 43/150 (28.67%), Postives = 75/150 (50.00%), Query Frame = 3 
Query:  330 NCIKF-KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            +CI+F +      + ++ +  GC + VG              C+    V+HE  H+LGF HE TR DRDN++ + + N+  ++  NF         TPYD+ S+MQY   AF+ NG  T+  +  N  ++  + ++++ D  ++   Y C
Sbjct:  116 SCIRFFRRSNERDYISIESRSGCYSYVGRQGNVQTVSLARSGCLYHSTVQHELLHALGFNHEQTRSDRDNHIQVIWENILDDMKYNFNKINTLNQGTPYDYKSVMQYERYAFSKNGYPTMIPIPNNNAELGKSTQMSQNDITRLNRLYQC 265
[Close]
BLAST of Ast7 protein vs. Ensembl Xenopus
Match: polr2l.2 (polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa, gene 2 [Source:Xenbase;Acc:XB-GENE-5898446])

HSP 1 Score: 86.6557 bits (213), Expect = 1.013e-18
Identity = 50/190 (26.32%), Postives = 88/190 (46.32%), Query Frame = 3 
Query:  228 WPKN-----ELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGV-FATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKN---GGKIAFNMEVTKLDAKKVRDYYGC 770
            WPK+      + Y     + D  K+ +RD L  +      C+ F E  +   F  +V+  GC +++G          ++  C+ K  ++HE  H+LGF HE +R DRD  +++ +  + ++   +F + +      PYD+ S+M Y   AF+    +  LR        I     ++ LD  KV++ YGC
Sbjct:   71 WPKSADGTVRVPYRLSADYSDNEKSSIRDALLEFNTL--TCVHFVERSTEEDFLDIVSDSGCWSSIGRTGGAQTLSLMSSGCLAKGIIQHEVDHALGFYHEQSRSDRDTYIDVLWQYICESDWGSFEMVDTDNLDLPYDYSSVMHYGWYAFSNTSGQPSLRPKPDPTANIGQRYGLSPLDVSKVKELYGC 258
[Close]
BLAST of Ast7 protein vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 82.0333 bits (201), Expect = 3.752e-17
Identity = 55/192 (28.65%), Postives = 84/192 (43.75%), Query Frame = 3 
Query:  228 WPKNE----LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKF----KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFT-CNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            W K+     + YT D  +     N +   ++ Y      C++F     EE    +  + +G GC + +G      +       C ++    HE  H+LGF HEH+R DRDN +NI +  +      NF   E     T YD+ SIM YP+ AF+   G+ TI+ K    +       +T LD  K+   Y C
Sbjct:   79 WQKSNGIVYVPYTLDKQYSSDQINTITSAMEVYSTL--TCVQFVPYTDEED---YIAITSGDGCWSFMGRQGGAQVVSVEKGYCTSEGTTMHELNHALGFVHEHSRSDRDNYVNIMYQYISSGDDVNFEKMETNNLDTTYDYRSIMHYPAWAFSNTTGQNTIVAKPNPNVTIGAGNTMTSLDIIKINRLYEC 265
[Close]
BLAST of Ast7 protein vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 82.0333 bits (201), Expect = 4.449e-17
Identity = 50/180 (27.78%), Postives = 80/180 (44.44%), Query Frame = 3 
Query:  243 LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKE-EKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFT-CNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            + YT D  + +   N +   ++ Y      C++F        +  + +G GC + +G      +       C ++    HE  H+LGF HEH+R DRDN ++I +  +      NF   E     T YD+ SIM YP+ AF+   G+ TI+ K    +       +T LD  K+   Y C
Sbjct:   83 VPYTLDEQYSNNEVNTIMTAMQVYATL--TCVQFVPYTDEDDYIAITSGDGCWSYMGRQGGAQVVSVQKTYCTSEGTTMHELNHALGFVHEHSRSDRDNYVDIMYQYISPGDIVNFKKMETNNLNTTYDYHSIMHYPAWAFSNTTGQNTIVAKPNPNVTIGAGNTMTSLDIIKINRLYEC 260
[Close]
BLAST of Ast7 protein vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 81.6481 bits (200), Expect = 5.346e-17
Identity = 50/180 (27.78%), Postives = 80/180 (44.44%), Query Frame = 3 
Query:  243 LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKE-EKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFT-CNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            + YT D  + +   N +   ++ Y      C++F        +  + +G GC + +G      +       C ++    HE  H+LGF HEH+R DRDN ++I +  +      NF   E     T YD+ SIM YP+ AF+   G+ TI+ K    +       +T LD  K+   Y C
Sbjct:  123 VPYTLDEQYSNNEVNTIMTAMQVYATL--TCVQFVPYTDEDDYIAITSGDGCWSYMGRQGGAQVVSVQKTYCTSEGTTMHELNHALGFVHEHSRSDRDNYVDIMYQYISPGDIVNFKKMETNNLNTTYDYHSIMHYPAWAFSNTTGQNTIVAKPNPNVTIGAGNTMTSLDIIKINRLYEC 300
[Close]
BLAST of Ast7 protein vs. Ensembl Xenopus
Match: ENSXETT00000022029.1 (astacin-like metalloendopeptidase [Source:NCBI gene;Acc:100496745])

HSP 1 Score: 80.8777 bits (198), Expect = 1.006e-16
Identity = 53/189 (28.04%), Postives = 83/189 (43.92%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPV-RDFLKAYEKELG--NCIKFKE-EKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNG---GKIAFNMEVTKLDAKKVRDYYGCA 773
            WP++       P     N N   RD ++A   E+    CI+F        +  +    GC + +G          +   C++   ++HE  HSLGFQHE  R DRDN +NI ++N+  +   NF         +PYD+ S+M Y   AF  N  +  L   G     I   + ++ LD +K+   Y C+
Sbjct:   24 WPRSRKGLVLVPYTLSNNYNSTERDIIRAAMDEVTVLTCIQFVTYNNESDYLRIRPYDGCWSYIGRVGGAQDVSLMKTGCLHHGVIQHELLHSLGFQHEQCRSDRDNYININWDNISHDKERNFLKMNTQNLGSPYDYLSVMHYGKFAFATNSGKPTLEPKGNPSAMIGQRVGLSSLDVEKINRLYQCS 212
[Close]
BLAST of Ast7 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 82.4185 bits (202), Expect = 2.120e-17
Identity = 59/216 (27.31%), Postives = 95/216 (43.98%), Query Frame = 3 
Query:  159 GDMVL-SPVEILKINGYKAADGEKWPKN-----ELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKE-EKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRN--VKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGCA 773
            GD++  SP  +L +         KWPK      E+ +     + + ++  + D    +E+    CI+F        F +++   GC + VG +    + V+LA  C+ K    V HE  H LGF HEH+R DRD  + + +N +      NF          PYD+ S+M Y   AF+  G+ TI  L  +   I     ++  D  +V   Y C+
Sbjct:   76 GDIIRPSPFRLLSVTN------NKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSILPMAGCFSGVGRSGGMQV-VSLAPTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNCS 282
[Close]
BLAST of Ast7 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 81.6481 bits (200), Expect = 3.496e-17
Identity = 59/216 (27.31%), Postives = 95/216 (43.98%), Query Frame = 3 
Query:  159 GDMVL-SPVEILKINGYKAADGEKWPKN-----ELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKE-EKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRN--VKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGCA 773
            GD++  SP  +L +         KWPK      E+ +     + + ++  + D    +E+    CI+F        F +++   GC + VG +    + V+LA  C+ K    V HE  H LGF HEH+R DRD  + + +N +      NF          PYD+ S+M Y   AF+  G+ TI  L  +   I     ++  D  +V   Y C+
Sbjct:   55 GDIIRPSPFRLLSVTN------NKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSILPMAGCFSGVGRSGGMQV-VSLAPTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNCS 261
[Close]
BLAST of Ast7 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 81.6481 bits (200), Expect = 3.496e-17
Identity = 59/216 (27.31%), Postives = 95/216 (43.98%), Query Frame = 3 
Query:  159 GDMVL-SPVEILKINGYKAADGEKWPKN-----ELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKE-EKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRN--VKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGCA 773
            GD++  SP  +L +         KWPK      E+ +     + + ++  + D    +E+    CI+F        F +++   GC + VG +    + V+LA  C+ K    V HE  H LGF HEH+R DRD  + + +N +      NF          PYD+ S+M Y   AF+  G+ TI  L  +   I     ++  D  +V   Y C+
Sbjct:   55 GDIIRPSPFRLLSVTN------NKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSILPMAGCFSGVGRSGGMQV-VSLAPTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNCS 261
[Close]
BLAST of Ast7 protein vs. Ensembl Mouse
Match: Mep1b (meprin 1 beta [Source:MGI Symbol;Acc:MGI:96964])

HSP 1 Score: 78.5666 bits (192), Expect = 4.724e-16
Identity = 57/205 (27.80%), Postives = 95/205 (46.34%), Query Frame = 3 
Query:  189 LKINGYKA--ADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEK-ELGNCIKFKEEKSGV-FATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTT---PYDFDSIMQYPSEAFTCNGKETILRKNG---GKIAFNMEVTKLDAKKVRDYYGCA 773
            L+ NG  +   D ++WP + + Y  + S     K  +   L A+E+  L  CI FK       + +V  G GC ++VG       ++++   C     V+HEF H+LGF HE +R DRD+ + I ++ ++     NF +     + +   PYD+ S+M Y   AF    + TI+ +       I   M+ +  D  K+   Y C 
Sbjct:   57 LEANGKNSIIGDHKRWP-HTIPYVLEDSLEMNAKGVI---LNAFERYRLKTCIDFKPWSGEANYISVFKGSGCWSSVGNIHAGKQELSIGTNCDRIATVQHEFLHALGFWHEQSRADRDDYVIIVWDRIQPGKEHNFNIYNDSVSDSLNVPYDYTSVMHYSKTAFQNGTESTIVTRISEFEDVIGQRMDFSDYDLLKLNQLYNCT 257
[Close]
BLAST of Ast7 protein vs. Ensembl Mouse
Match: Tll1 (tolloid-like [Source:MGI Symbol;Acc:MGI:106923])

HSP 1 Score: 77.7962 bits (190), Expect = 1.149e-15
Identity = 58/192 (30.21%), Postives = 83/192 (43.23%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVT--GGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTT---PYDFDSIMQYPSEAFTCNG-KETIL--RKNGG---KIAFNMEVTKLDAKKVRDYYGC 770
            WP   + Y    +F    +   +  ++ +EK    C+ F E        V T    GC + VG     P  +++   C     V HE  H +GF HEHTRPDRDN++ I   N++     NF   E  +  +    YDFDSIM Y    F+     +TIL  R + G    I     ++K D  + R  Y C
Sbjct:  157 WPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFTERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of Ast7 protein vs. UniProt/SwissProt
Match: sp|P31579|LCE_ORYLA (Low choriolytic enzyme OS=Oryzias latipes OX=8090 GN=lce PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.017e-19
Identity = 62/212 (29.25%), Postives = 99/212 (46.70%), Query Frame = 3 
Query:  159 GDMVLSPVEILKINGYKAADGEKWPKN-----ELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKF-KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            GD+VL P     +  + A D  +WPK+     ++ Y    ++    K  +R+ +K + ++   CI F        + ++    GC + +GY     + V     C+    ++HE  H+LGF HEHTR DRD ++ I + N+ K+   NF   +     TPYD+ SIM Y   AF  + KETI      K A      ++ +D  +V   Y C
Sbjct:   63 GDLVL-PKTRNAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRFGCKSMMGYVGDKQVVVLQRFGCIKHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
[Close]
BLAST of Ast7 protein vs. UniProt/SwissProt
Match: sp|Q19269|NAS14_CAEEL (Zinc metalloproteinase nas-14 OS=Caenorhabditis elegans OX=6239 GN=nas-14 PE=2 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 2.937e-18
Identity = 54/190 (28.42%), Postives = 93/190 (48.95%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVT----GGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANF--YLCEGCKTT-TPYDFDSIMQYPSEAFTCNGKETILRK-NGGKIAFNMEVTKLDAKKVRDYYGCA 773
            WP+ ++ Y  +    +  +  +      Y+ +   C++F  +    F  +        GCS+ VG            D C +K  + HE  H+LGF HEH+R DRD+ ++I  +N++  +  NF  Y  +   +   PYD++S+M Y   AF+ NGK TI+ K N   +    +++++D+KKV   Y C 
Sbjct:  125 WPEGQVPYMLEEGMTNDQRTAIAQAFDEYKTK--TCVRFVPKTDDDFDYIYVKRNVAFGCSSYVGRAGGNQTVSLEVDKCFSKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNIRPGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFSRNGKPTIIPKDNEADVGQRYKLSEMDSKKVNKLYQCG 312
[Close]
BLAST of Ast7 protein vs. UniProt/SwissProt
Match: sp|Q9U3S9|NAS6_CAEEL (Zinc metalloproteinase nas-6 OS=Caenorhabditis elegans OX=6239 GN=nas-6 PE=2 SV=2)

HSP 1 Score: 86.2705 bits (212), Expect = 3.077e-18
Identity = 57/189 (30.16%), Postives = 89/189 (47.09%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEK-ELGNCIKF-KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTP---YDFDSIMQYPSEAFTCNGKETILRKNGG---KIAFNMEVTKLDAKKVRDYYGC 770
            W    + Y  D +F   + N ++   KA++      CI+F K E    +  +V G GC + VG       +++L   C     + HE  HS+GF HEH+R DRD+++ I ++N+   + + F              YD+ SIM Y S AF+ NG+ TI     G    I   M+++ LD  K+   Y C
Sbjct:   81 WEGGVIPYEMDTAF---SPNEIKILEKAFDSYRRTTCIRFEKREGQTDYLNIVKGYGCYSQVGRTGG-KQEISLGRGCFFHEIIVHELMHSVGFWHEHSRADRDDHIKINWDNILPGMKSQFDKISAVLQDLQGENYDYKSIMHYDSTAFSRNGRNTIETVENGFTQVIGTAMDLSPLDIVKINKLYSC 265
[Close]
BLAST of Ast7 protein vs. UniProt/SwissProt
Match: sp|P55113|NAS7_CAEEL (Zinc metalloproteinase nas-7 OS=Caenorhabditis elegans OX=6239 GN=nas-7 PE=2 SV=2)

HSP 1 Score: 86.6557 bits (213), Expect = 3.263e-18
Identity = 59/199 (29.65%), Postives = 94/199 (47.24%), Query Frame = 3 
Query:  192 KINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTG--GGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTT---TPYDFDSIMQYPSEAFTCNGKETILRK-NGGKIAFNMEVTKLDAKKVRDYYGC 770
            K NG   A  + WP   + Y   P +    +  +   +K Y ++   CI+F   ++G    +  G   GC + VG  S   + ++L + C+    + HE  H +GF HEH R DRDN ++I + N+ +     F   +  KT+    PYD+ SI+ Y S AF+ NG  T+L K     I    + + +D  K+   Y C
Sbjct:   78 KRNGVSRA-AKLWPNARIPYAISPHYSPHERALLAKAVKQYHEK--TCIRFVPRQTGEPDYLFIGKVDGCFSEVGRTSGVQV-LSLDNGCMEYATIIHEMMHVVGFYHEHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFSKNGFPTMLPKVKSATIGNARDFSDVDISKINRMYNC 272
[Close]
BLAST of Ast7 protein vs. UniProt/SwissProt
Match: sp|Q6HA08|ASTL_HUMAN (Astacin-like metalloendopeptidase OS=Homo sapiens OX=9606 GN=ASTL PE=1 SV=4)

HSP 1 Score: 86.6557 bits (213), Expect = 3.997e-18
Identity = 62/218 (28.44%), Postives = 102/218 (46.79%), Query Frame = 3 
Query:  159 GDMV-LSPVEILKINGYKAADGEKWPKN-----ELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKF---KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRN--VKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGCA 773
            GD++  SP  +L      +A   KWP       E+ +     + + ++  + + L  +E+    CI+F   ++++   F +++   GC ++VG +    + V+LA  C+ K    V HE  H LGF HEHTR DRD  + + +N +      NF   +     TPYD+ S+M Y   AF+  G  TI  L      I     ++  D  +V   YGC+
Sbjct:   76 GDIIRPSPFRLL------SATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERS--TCIRFVTYQDQRD--FISIIPMYGCFSSVGRSGGMQV-VSLAPTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSQSSNMLTPYDYSSVMHYGRLAFSRRGLPTITPLWAPSVHIGQRWNLSASDITRVLKLYGCS 282
[Close]
BLAST of Ast7 protein vs. TrEMBL
Match: A0A060Q715 (Metalloendopeptidase (Fragment) OS=Schmidtea mediterranea OX=79327 GN=Ast7 PE=2 SV=1)

HSP 1 Score: 484.952 bits (1247), Expect = 1.434e-172
Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 3 
Query:   72 CFLLISLFSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGCA 773
            CFLLI+LFSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGCA
Sbjct:    3 CFLLITLFSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGCA 236
[Close]
BLAST of Ast7 protein vs. TrEMBL
Match: A0A060Q6Y6 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast4 PE=2 SV=1)

HSP 1 Score: 377.096 bits (967), Expect = 8.240e-130
Identity = 177/233 (75.97%), Postives = 198/233 (84.98%), Query Frame = 3 
Query:   75 FLLISL-FSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            F+ +S+   LC AQCGCTQKGIKI++FNVGDMVLS  ++L+ING+KAA G  WP   LRYTFDPS+PDKNKN +R+FLK YEKELGNC++F EEKS V+ATVV GGGCSANVGYN  YPL +TL DPCVN+RNVKHEFTH LGF HEHTRPDRD  L I+F NV   LC NFYLCEGCK TT YDFDSIMQYPSEAF+CNGK+TIL+KNGGKI FNMEVTKLDAKKVRDYYGC
Sbjct:    9 FITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNAIREFLKIYEKELGNCLRFLEEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 241
[Close]
BLAST of Ast7 protein vs. TrEMBL
Match: A0A060Q786 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast6 PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 1.530e-48
Identity = 90/241 (37.34%), Postives = 141/241 (58.51%), Query Frame = 3 
Query:   75 FLLISLFSLCYAQCGCT-QKGIK--IADFNVGDMVLSPVEILKINGYKA--ADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVT-GGGCSANVGYNSRYPLKVTLADP-CV-NKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTC-NGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
             L+++  S    QCGC  QK +K  +    +GDMVL   +I  ING+KA  ++ ++WP N++ ++F P+FP   K  VR+ L   +K+LGNC+KF E  +  +  V +   GC + +GY       + L +P C+ +K  VKHEF H+LGF H H R DRDN++ I+++ +  + C+ F  CEGC    PY+ +S+M YPS  F C  G+  + +++GG I +N   ++ D   VR +Y C
Sbjct:    8 LLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248
[Close]
BLAST of Ast7 protein vs. TrEMBL
Match: A0A060Q785 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast1 PE=2 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 6.333e-48
Identity = 90/241 (37.34%), Postives = 140/241 (58.09%), Query Frame = 3 
Query:   78 LLISLFSLCYAQCGCTQKGI---KIADFNVGDMVLSPVEILKINGYKA--ADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGG-GCSANVGYNSRYPLK-VTLADP-CV-NKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKE-TILRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            L I++      QCGC ++ +    +    +GDM+L   +I  ING+K   A+ +KWP N + Y+F  +FP      VR  L   +++LGNC+KF E   G +  V +   GC + +GY +  P + + L +P C+ +K  VKHEF H+LGF H H R DRDN++ I +  ++ + C+ F  C+GC+   PY+  S+M Y SE F C+ +E  +LRK+GG I +N + T  D   +R++YGC
Sbjct:    9 LAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 249
[Close]
BLAST of Ast7 protein vs. TrEMBL
Match: A0A060Q714 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast2 PE=2 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 8.688e-48
Identity = 90/245 (36.73%), Postives = 143/245 (58.37%), Query Frame = 3 
Query:   75 FLLISLFSLCYA--QCGCTQKGIKIA-----DFNVGDMVLSPVEILKINGYKA--ADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTG-GGCSANVGYNSRYPLKVTLADP-CV-NKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKE-TILRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
             LL+    + YA  QCGC ++ +K+      +  +GDM+L   +I  ING+K   A+ +KWP N + YTF  +FP      VR  L   +K+L NC+KF+E ++G +  V +   GC + +G        + L +P C+ +K  +KHEF H+LGF H H R DRDN++ I+++ +  + C+ F  C+GC+   PY+ +S+M Y SE F CN  E  +++KNGG I +N E T  D + ++++Y C
Sbjct:    6 LLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 250
[Close]
BLAST of Ast7 protein vs. Ensembl Cavefish
Match: c6ast3 (six-cysteine containing astacin protease 3 [Source:ZFIN;Acc:ZDB-GENE-050320-99])

HSP 1 Score: 92.4337 bits (228), Expect = 8.856e-22
Identity = 53/192 (27.60%), Postives = 93/192 (48.44%), Query Frame = 3 
Query:  219 GEKWPKNE-----LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGV-FATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            G  WPK+      + Y     F  +    ++  L ++     +CI+FK   +   + ++ +  GC + VG            + C+  + V+HE  H+LGF HE TR DRDN++ + + N+   +  NF         TPYD++S+MQY   AF+ NGK T++      +AF    ++++ D  +++  Y C
Sbjct:   58 GCMWPKSNDGKVYVPYVISNQFASREVQVIQRGLDSFSS--FSCIRFKRRTNERDYLSIESRNGCYSYVGRRGNAQTVSLARNGCIYHKTVQHELLHALGFNHEQTRSDRDNHIRVAWENIVDTMKHNFNKIATLNQGTPYDYNSVMQYHKTAFSKNGKPTMIPIPNANVAFGQATQMSQNDITRLKKLYKC 247
[Close]
BLAST of Ast7 protein vs. Ensembl Cavefish
Match: ENSAMXT00000038400.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000034.1:1231347:1242851:1 gene:ENSAMXG00000020828.2 transcript:ENSAMXT00000038400.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 88.5817 bits (218), Expect = 1.514e-20
Identity = 55/209 (26.32%), Postives = 99/209 (47.37%), Query Frame = 3 
Query:  183 EILKINGYKAAD-----GEKWPKNE-----LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGV-FATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            +I  +N  + AD       KWPK+      + Y     F  +    ++  L ++     +CI+F    +   + ++ +  GC + VG            + C+    V+HE  H+LGF HE TR DRDN++ + ++N+  N+  NF         T YD++S+MQY   AF+ NG+ T++      ++F    ++++ D  ++   YGC
Sbjct:   49 DIAVVNSERNADPCTSQSCKWPKSSDGKVYVPYVIVNHFTSRELQVIQRGLDSFSSV--SCIRFTPRSNQRDYVSIESRSGCYSYVGRIGNAQTVSLDRNGCIYHNTVQHELLHALGFNHEQTRSDRDNHIRVVWDNIIDNMKHNFNKINTLNQGTAYDYNSVMQYHRTAFSKNGQATMVPIPNSNVSFGQATQMSQSDITRINRLYGC 255
[Close]
BLAST of Ast7 protein vs. Ensembl Cavefish
Match: ENSAMXT00000044667.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000034.1:1231347:1242743:1 gene:ENSAMXG00000020828.2 transcript:ENSAMXT00000044667.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 88.5817 bits (218), Expect = 2.325e-20
Identity = 55/209 (26.32%), Postives = 99/209 (47.37%), Query Frame = 3 
Query:  183 EILKINGYKAAD-----GEKWPKNE-----LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGV-FATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            +I  +N  + AD       KWPK+      + Y     F  +    ++  L ++     +CI+F    +   + ++ +  GC + VG            + C+    V+HE  H+LGF HE TR DRDN++ + ++N+  N+  NF         T YD++S+MQY   AF+ NG+ T++      ++F    ++++ D  ++   YGC
Sbjct:   49 DIAVVNSERNADPCTSQSCKWPKSSDGKVYVPYVIVNHFTSRELQVIQRGLDSFSSV--SCIRFTPRSNQRDYVSIESRSGCYSYVGRIGNAQTVSLDRNGCIYHNTVQHELLHALGFNHEQTRSDRDNHIRVVWDNIIDNMKHNFNKINTLNQGTAYDYNSVMQYHRTAFSKNGQATMVPIPNSNVSFGQATQMSQSDITRINRLYGC 255
[Close]
BLAST of Ast7 protein vs. Ensembl Cavefish
Match: ENSAMXT00000021576.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000034.1:1237857:1242743:1 gene:ENSAMXG00000020828.2 transcript:ENSAMXT00000021576.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.8113 bits (216), Expect = 2.971e-20
Identity = 55/209 (26.32%), Postives = 99/209 (47.37%), Query Frame = 3 
Query:  183 EILKINGYKAAD-----GEKWPKNE-----LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGV-FATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            +I  +N  + AD       KWPK+      + Y     F  +    ++  L ++     +CI+F    +   + ++ +  GC + VG            + C+    V+HE  H+LGF HE TR DRDN++ + ++N+  N+  NF         T YD++S+MQY   AF+ NG+ T++      ++F    ++++ D  ++   YGC
Sbjct:   28 DIAVVNSERNADPCTSQSCKWPKSSDGKVYVPYVIVNHFTSRELQVIQRGLDSFSSV--SCIRFTPRSNQRDYVSIESRSGCYSYVGRIGNAQTVSLDRNGCIYHNTVQHELLHALGFNHEQTRSDRDNHIRVVWDNIIDNMKHNFNKINTLNQGTAYDYNSVMQYHRTAFSKNGQATMVPIPNSNVSFGQATQMSQSDITRINRLYGC 234
[Close]
BLAST of Ast7 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:103036149])

HSP 1 Score: 88.1965 bits (217), Expect = 3.387e-20
Identity = 51/160 (31.87%), Postives = 79/160 (49.38%), Query Frame = 3 
Query:  228 WPKNE-----LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKF-KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETIL 689
            WPKN+     + Y     F  K K  + D LK +      CI+F   ++   +  +    GC ++VG              C+ KR V+HEF H+LGF HE +R DRD ++ I + N+  +   NF   +     TPYD+ S+M Y  +AF+ NG+ T++
Sbjct:   92 WPKNKNGIVYVPYIISNQFCPKEKKLITDILKTFRN--STCIQFVPRDREEDYIHIQNLKGCYSSVGRRGGEQTLSLSRQGCLYKRAVQHEFIHALGFHHEQSRSDRDQHIRIVYENIDPDKKHNFNKVDTNNLKTPYDYGSVMHYGRDAFSNNGQPTMI 249
[Close]
BLAST of Ast7 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007253.1 (pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1 gene:ENSPMAG00000006538.1 transcript:ENSPMAT00000007253.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 73.559 bits (179), Expect = 4.081e-15
Identity = 48/151 (31.79%), Postives = 67/151 (44.37%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVT--GGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTT---PYDFDSIMQYPSEAFT 665
            WP   + Y    +F    K   R  ++ +EK    C+ F E        V T    GC + VG     P  +++   C     V HE  H +GF HEHTRPDRD+++ I   N++     NF   E  +  +    YDFDSIM Y    F+
Sbjct:   22 WPGGVIPYIVGGNFTGSQKAMFRQAMRHWEKH--TCVTFIERTDEESYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIKANIQPGQEYNFLKMEPGEVDSLGESYDFDSIMHYARNTFS 170
[Close]
BLAST of Ast7 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004913.1 (pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 gene:ENSPMAG00000004462.1 transcript:ENSPMAT00000004913.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 2.584e-8
Identity = 27/101 (26.73%), Postives = 42/101 (41.58%), Query Frame = 3 
Query:  435 VTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTT-TPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTK 734
            V++   C     V HE  H++G+ HE +RPDRD  +++   NV+      F +         PYD  S+M Y      C  ++       G +    EV  
Sbjct:   15 VSIGRGCEGVGTVAHELGHAVGYVHEQSRPDRDAFISVLEGNVQDGQLPQFAVYSATDPRGVPYDLKSVMHYSRNVRACGTRDRPCGIPAGIVRCECEVCS 115
[Close]
BLAST of Ast7 protein vs. Ensembl Nematostella
Match: EDO41420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4I5])

HSP 1 Score: 119.398 bits (298), Expect = 6.708e-33
Identity = 76/227 (33.48%), Postives = 113/227 (49.78%), Query Frame = 3 
Query:  135 IKIADFNVGDMVLSPVEILK------INGYKAADGEK---WPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSG-VFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTT---TPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFN-MEVTKLDAKKVRDYYGCA 773
            ++  D   GDM L+  + L       I+   A  G K   WP+  + YT D S  ++  N +R  +K +      CI+FKE  +   +A    G GCS+ VG   R    ++LA  C     V HE  H+LGF HE +RPDRDN + I +NN+  N+  NF +          TPYD+ S+M Y   AF+ NG+ TI  K  G    N  +++K+D +++   Y C+
Sbjct:    1 VEDPDLFEGDMRLTDAQRLAAQMGQDIDSPSARGGIKTGRWPEGVMAYTIDRSLSNRAMNAIRAGMKMWTDN--TCIRFKERTNEYAYARFWRGRGCSSMVGRTGRRQ-DISLAGGCWYPGTVAHEIGHALGFYHEQSRPDRDNYVTIHWNNISPNMKFNFNMYPRSTIDSLGTPYDYRSVMHYQGNAFSSNGRATITAKQSGVTLGNRRQLSKVDIQQMNLMYKCS 224
[Close]
BLAST of Ast7 protein vs. Ensembl Nematostella
Match: EDO37770 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEU5])

HSP 1 Score: 108.997 bits (271), Expect = 4.145e-28
Identity = 78/247 (31.58%), Postives = 116/247 (46.96%), Query Frame = 3 
Query:   72 CFLLISLFSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKING--YKAADG-----EKWPKNELRYTFDPSFPDKNKNPVRDFLK--AYEKELGNCIKFKEEKSGV-FATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANF--YLCEGCKT-TTPYDFDSIMQYPSEAFTCNGKETIL-RKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            CF  ISL S           G   +D   GDM+L+P +   +N     A+ G     +KWP   L Y       D    P    ++    E E   C++F++  +   +     G GC + VG   R    ++L   C +K    HE  H+LGF HE +RPDRDN + + ++N+K +   NF  Y  +   T  TPYDF S+M Y +  F+ NG+ TI  +K+G KI     ++ LD  ++   YGC
Sbjct:    8 CFTAISLVSSASID---ENAGNPRSDLFEGDMILTPDQQQAVNNGDLGASRGAPIHDKKWPNAILPYEIRS---DLKGTPAEGVIQNAMREWESKTCVRFQKRTTQKNYVYFFKGDGCWSYVGMLDRGAQAISLGSGCWHKGTAAHEIGHALGFYHEQSRPDRDNYVTVHWDNIKTDSKHNFNKYSADRINTFGTPYDFGSLMHYGTNYFSVNGRATITPKKSGAKIGQRDYLSDLDVHQMNLRYGC 248
[Close]
BLAST of Ast7 protein vs. Ensembl Nematostella
Match: EDO34806 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNB7])

HSP 1 Score: 101.293 bits (251), Expect = 2.109e-26
Identity = 55/153 (35.95%), Postives = 86/153 (56.21%), Query Frame = 3 
Query:  330 NCIKFKEEKSG--VFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTT---TPYDFDSIMQYPSEAFTCNGKETILRKNGG-KIAFNMEVTKLDAKKVRDYYGC 770
             CI+ + +K+G   + ++  GGGC + VG       +++LA  C +K  V HE  H+LGF HE +RPDRD+ + I+F N++     NF +    + T   T YDF+S+M Y   AF+ NG+ TI+ K  G  +     ++ LD  +V  +YGC
Sbjct:   14 TCIRLRPKKAGETAYLSIFKGGGCWSYVGRTGS-KQQLSLAGGCWHKGIVIHEIAHALGFFHEQSRPDRDHFVTIKFENIQDGKEGNFRMHSTSQVTTHGTTYDFESVMHYGGRAFSKNGQPTIVAKKAGVNLGQRHGLSHLDTIQVNIHYGC 165
[Close]
BLAST of Ast7 protein vs. Ensembl Nematostella
Match: EDO39627 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9K8])

HSP 1 Score: 102.834 bits (255), Expect = 3.633e-26
Identity = 69/213 (32.39%), Postives = 105/213 (49.30%), Query Frame = 3 
Query:  159 GDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFK----EEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTT---TPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNME-VTKLDAKKVRDYYGCA 773
            GD+VL+  +  ++N   A     W    + Y  DPS    +           E     CI+F+     +K G+   +  GGGC + VG  S    +++LA  C +K  V HEF H+LGF HE +RPDRD  + I+  N++  + +NF        T   TPYD+ S+M Y + AF+ NGK TI+ K  G I      ++ +D K++  +Y C 
Sbjct:   27 GDIVLTDEQKTQLNSRAAIKTNHWTNGVVPYELDPSISSSSYAVSVINSAIAEYNQKTCIRFRPRVPSDKYGL--KIFKGGGCWSYVGM-SMPTQQLSLAGGCWSKGIVIHEFAHALGFYHEQSRPDRDQFVEIKTENIRDGVASNFKKHSFNYVTTHGTPYDYGSVMHYGAYAFSKNGKPTIVAKQPGVILGQRRGLSDIDTKQINIHYRCG 236
[Close]
BLAST of Ast7 protein vs. Ensembl Nematostella
Match: EDO42421 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1T1])

HSP 1 Score: 100.138 bits (248), Expect = 7.292e-26
Identity = 62/190 (32.63%), Postives = 98/190 (51.58%), Query Frame = 3 
Query:  228 WPKNELRYTFDPSFPDKNK--NPVRDFLKAYEKELGNCIKFKEEKSGV--FATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFY---LCEGCKTTTPYDFDSIMQYPSEAFTCNGKETIL-RKNGGKIAFNMEVTKLDAKKVRDYYGCA 773
            WP   + Y  DP F   ++    +   +  YEK    CI+F+++ +    +  +   GGC ++VG        ++L+  C +K  + HE  H+LGF HE TRPDRD+ + I F N++  + +NF      E     TPYD+ SIM Y  + F+ NGK TI+ +K G K      ++ +D K+V   Y C+
Sbjct:   12 WPGGVVVYELDPRFERSSRAMEKLTQAISDYEKY--TCIRFRKKTANDNDYVYIKDRGGCRSHVGRKGGKQY-LSLSSGCWHKGTIIHELGHALGFFHEQTRPDRDDYVTILFENIRLGMASNFMKHSAGEVDSLGTPYDYGSIMHYGPKIFSANGKPTIVAKKPGVKFGQRDGLSPIDIKQVNLLYKCS 198
[Close]
BLAST of Ast7 protein vs. Ensembl Medaka
Match: lce (protease [Source:NCBI gene;Acc:100049451])

HSP 1 Score: 89.7373 bits (221), Expect = 7.629e-21
Identity = 62/212 (29.25%), Postives = 99/212 (46.70%), Query Frame = 3 
Query:  159 GDMVLSPVEILKINGYKAADGEKWPKN-----ELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKF-KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            GD+VL P     +  + A D  +WPK+     ++ Y    ++    K  +R+ +K + ++   CI F        + ++    GC + +GY     + V     C+    ++HE  H+LGF HEHTR DRD ++ I + N+ K+   NF   +     TPYD+ SIM Y   AF  + KETI      K A      ++ +D  +V   Y C
Sbjct:   63 GDLVL-PKTRNAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRFGCKSMMGYVGDKQVVVLQRFGCIKHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
[Close]
BLAST of Ast7 protein vs. Ensembl Medaka
Match: lce (protease [Source:NCBI gene;Acc:100049451])

HSP 1 Score: 89.3521 bits (220), Expect = 1.135e-20
Identity = 62/212 (29.25%), Postives = 99/212 (46.70%), Query Frame = 3 
Query:  159 GDMVLSPVEILKINGYKAADGEKWPKN-----ELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKF-KEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            GD+VL P     +  + A D  +WPK+     ++ Y    ++    K  +R+ +K + ++   CI F        + ++    GC + +GY     + V     C+    ++HE  H+LGF HEHTR DRD ++ I + N+ K+   NF   +     TPYD+ SIM Y   AF  + KETI      K A      ++ +D  +V   Y C
Sbjct:   63 GDLVL-PKTRNAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRFGCKSMMGYVGDKQVVVLQRFGCIKHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
[Close]
BLAST of Ast7 protein vs. Ensembl Medaka
Match: mc6ast2 (astacin like metallo-protease [Source:NCBI gene;Acc:100049316])

HSP 1 Score: 85.1149 bits (209), Expect = 3.491e-19
Identity = 45/151 (29.80%), Postives = 78/151 (51.66%), Query Frame = 3 
Query:  330 NCIKFKEEKSG--VFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNM--EVTKLDAKKVRDYYGC 770
            +CI+F+  ++G   + ++ +  GC + VG              C+    V+HE  H+LGF HE TR DRDN++ + + N+  ++  NFY  +     TPYD++S+MQY   AF+ N   T+       ++F    +++K D  ++   Y C
Sbjct:  118 SCIRFRPYQNGDHEWLSIESRNGCYSYVGRQGGGQTVSLSRQGCLYHSTVQHELLHALGFNHEQTRSDRDNHIKVHWENIIDDMEYNFYKIDTLNQGTPYDYNSVMQYERYAFSKNNLPTMEPIPDSNVSFGQATQMSKNDIDRLNRLYNC 268
[Close]
BLAST of Ast7 protein vs. Ensembl Medaka
Match: mnep (nephrosin [Source:NCBI gene;Acc:100049315])

HSP 1 Score: 85.1149 bits (209), Expect = 6.055e-19
Identity = 64/227 (28.19%), Postives = 102/227 (44.93%), Query Frame = 3 
Query:  144 ADFNVGDMVLSPV----EILKINGYKAAD-----GEKWPKNE-----LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGV-FATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGCA 773
            A+ N+G  +  P+    +I    G + AD     G  WPK+      + +     F  + ++ +   L+++E     CI+F   +S   F  + +  GC + VG      +   +   CV    ++HE  H+LGF HE TR DRD+++ I   NV      NF   E     TPYD++SIM Y   AF+ N + TI  +      I    E++  D  +V   Y C+
Sbjct:   45 ANVNIGKNLDDPLVMFGDIAVPTGLQNADPCTARGCLWPKSSDGNVYVPFRISGQFSSRERDTILQGLRSFEGS--TCIRFTPHQSQRDFVDIQSRTGCWSFVGRRGGGQVVSLMRQGCVFMGTIQHELLHALGFNHEQTRSDRDSHVRILLQNVISGQEHNFRKIETRNLGTPYDYNSIMHYGRFAFSRNREPTIVPIPDPNVPIGRATEMSSNDILRVNRLYECS 269
[Close]
BLAST of Ast7 protein vs. Ensembl Medaka
Match: mnep (nephrosin [Source:NCBI gene;Acc:100049315])

HSP 1 Score: 84.3445 bits (207), Expect = 8.266e-19
Identity = 64/226 (28.32%), Postives = 101/226 (44.69%), Query Frame = 3 
Query:  144 ADFNVGDMVLSPV----EILKINGYKAAD-----GEKWPKNE-----LRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGV-FATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETI--LRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            A+ N+G  +  P+    +I    G + AD     G  WPK+      + +     F  + ++ +   L+++E     CI+F   +S   F  + +  GC + VG      +   +   CV    ++HE  H+LGF HE TR DRD+++ I   NV      NF   E     TPYD++SIM Y   AF+ N + TI  +      I    E++  D  +V   Y C
Sbjct:   50 ANVNIGKNLDDPLVMFGDIAVPTGLQNADPCTARGCLWPKSSDGNVYVPFRISGQFSSRERDTILQGLRSFEGS--TCIRFTPHQSQRDFVDIQSRTGCWSFVGRRGGGQVVSLMRQGCVFMGTIQHELLHALGFNHEQTRSDRDSHVRILLQNVISGQEHNFRKIETRNLGTPYDYNSIMHYGRFAFSRNREPTIVPIPDPNVPIGRATEMSSNDILRVNRLYEC 273
[Close]
BLAST of Ast7 protein vs. Planmine SMEST
Match: SMESG000080475.1 (SMESG000080475.1)

HSP 1 Score: 485.723 bits (1249), Expect = 2.355e-176
Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 3 
Query:   72 CFLLISLFSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGCA 773
            CFLLI+LFSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGCA
Sbjct:    9 CFLLITLFSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGCA 242
[Close]
BLAST of Ast7 protein vs. Planmine SMEST
Match: SMESG000080471.1 (SMESG000080471.1)

HSP 1 Score: 377.096 bits (967), Expect = 2.291e-133
Identity = 177/233 (75.97%), Postives = 198/233 (84.98%), Query Frame = 3 
Query:   75 FLLISL-FSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            F+ +S+   LC AQCGCTQKGIKI++FNVGDMVLS  ++L+ING+KAA G  WP   LRYTFDPS+PDKNKN +R+FLK YEKELGNC++F EEKS V+ATVV GGGCSANVGYN  YPL +TL DPCVN+RNVKHEFTH LGF HEHTRPDRD  L I+F NV   LC NFYLCEGCK TT YDFDSIMQYPSEAF+CNGK+TIL+KNGGKI FNMEVTKLDAKKVRDYYGC
Sbjct:    9 FITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNAIREFLKIYEKELGNCLRFLEEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 241
[Close]
BLAST of Ast7 protein vs. Planmine SMEST
Match: SMESG000016662.1 (SMESG000016662.1)

HSP 1 Score: 332.798 bits (852), Expect = 6.385e-116
Identity = 153/234 (65.38%), Postives = 184/234 (78.63%), Query Frame = 3 
Query:   72 CFLLISL-FSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            CF+++ +   LCY+QCGC+QKGIK+ +FN+ DM+LS  +IL+ING+KAA    WP    RY FDPS+PD+NKN +R++LK YE ELGNC+KF E+KS V ATV+ G GCS+++GY+    L++TL D CVNKRNVKHEFTH LGF HEHTRPDRD  L I   NV    C NF+LCEGCKT+TPYDFDSIMQYPS+AFTCNGKETIL+ NG KI  NMEVTKLD KKVRDYY C
Sbjct:    8 CFIILGVGIELCYSQCGCSQKGIKVTNFNIDDMILSEKDILRINGFKAAQAALWPDKTARYIFDPSYPDRNKNRIREYLKIYEGELGNCLKFYEDKSDVHATVIMGKGCSSSIGYDKNKELRITLPDVCVNKRNVKHEFTHLLGFMHEHTRPDRDQYLKILLENVSPAYCHNFFLCEGCKTSTPYDFDSIMQYPSKAFTCNGKETILKINGDKIRSNMEVTKLDVKKVRDYYSC 241
[Close]
BLAST of Ast7 protein vs. Planmine SMEST
Match: SMESG000072791.1 (SMESG000072791.1)

HSP 1 Score: 170.629 bits (431), Expect = 4.254e-52
Identity = 90/241 (37.34%), Postives = 141/241 (58.51%), Query Frame = 3 
Query:   75 FLLISLFSLCYAQCGCT-QKGIK--IADFNVGDMVLSPVEILKINGYKA--ADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVT-GGGCSANVGYNSRYPLKVTLADP-CV-NKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTC-NGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
             L+++  S    QCGC  QK +K  +    +GDMVL   +I  ING+KA  ++ ++WP N++ ++F P+FP   K  VR+ L   +K+LGNC+KF E  +  +  V +   GC + +GY       + L +P C+ +K  VKHEF H+LGF H H R DRDN++ I+++ +  + C+ F  CEGC    PY+ +S+M YPS  F C  G+  + +++GG I +N   ++ D   VR +Y C
Sbjct:    8 LLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248
[Close]
BLAST of Ast7 protein vs. Planmine SMEST
Match: SMESG000015174.1 (SMESG000015174.1)

HSP 1 Score: 169.088 bits (427), Expect = 1.761e-51
Identity = 90/241 (37.34%), Postives = 140/241 (58.09%), Query Frame = 3 
Query:   78 LLISLFSLCYAQCGCTQKGI---KIADFNVGDMVLSPVEILKINGYKA--ADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGG-GCSANVGYNSRYPLK-VTLADP-CV-NKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKE-TILRKNGGKIAFNMEVTKLDAKKVRDYYGC 770
            L I++      QCGC ++ +    +    +GDM+L   +I  ING+K   A+ +KWP N + Y+F  +FP      VR  L   +++LGNC+KF E   G +  V +   GC + +GY +  P + + L +P C+ +K  VKHEF H+LGF H H R DRDN++ I +  ++ + C+ F  C+GC+   PY+  S+M Y SE F C+ +E  +LRK+GG I +N + T  D   +R++YGC
Sbjct:    9 LAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 249
The following BLAST results are available for this feature:
BLAST of Ast7 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ASTL8.323e-1928.44astacin like metalloendopeptidase [Source:HGNC Sym... [more]
TLL18.552e-1630.21tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843][more]
TLL13.390e-1530.21tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843][more]
TLL13.470e-1530.21tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843][more]
TLL24.595e-1530.05tolloid like 2 [Source:HGNC Symbol;Acc:HGNC:11844][more]
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BLAST of Ast7 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nas-142.309e-1928.42Zinc metalloproteinase nas-14 [Source:UniProtKB/S... [more]
nas-62.419e-1930.16Zinc metalloproteinase nas-6 [Source:UniProtKB/Sw... [more]
nas-72.565e-1929.65Zinc metalloproteinase nas-7 [Source:UniProtKB/Sw... [more]
nas-41.308e-1827.08Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-121.379e-1828.51Zinc metalloproteinase nas-12 [Source:UniProtKB/S... [more]
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BLAST of Ast7 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG118651.368e-2029.57gene:FBgn0028947 transcript:FBtr0080753[more]
CG67632.252e-1828.64gene:FBgn0039069 transcript:FBtr0084378[more]
CG67632.252e-1828.64gene:FBgn0039069 transcript:FBtr0334497[more]
CG69743.935e-1726.63gene:FBgn0038285 transcript:FBtr0083042[more]
CG152554.383e-1727.07gene:FBgn0028950 transcript:FBtr0080785[more]
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BLAST of Ast7 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CU326366.21.065e-1830.10pep chromosome:GRCz11:10:42887204:42898220:-1 gene... [more]
c6ast32.534e-1832.00six-cysteine containing astacin protease 3 [Source... [more]
c6ast32.534e-1832.00six-cysteine containing astacin protease 3 [Source... [more]
c6ast45.992e-1828.67six-cysteine containing astacin protease 4 [Source... [more]
c6ast45.992e-1828.67six-cysteine containing astacin protease 4 [Source... [more]
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BLAST of Ast7 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
polr2l.21.013e-1826.32polymerase (RNA) II (DNA directed) polypeptide L, ... [more]
golt1b3.752e-1728.65golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
golt1b4.449e-1727.78golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
golt1b5.346e-1727.78golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
ENSXETT00000022029.11.006e-1628.04astacin-like metalloendopeptidase [Source:NCBI gen... [more]
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BLAST of Ast7 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Astl2.120e-1727.31astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl3.496e-1727.31astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl3.496e-1727.31astacin-like metalloendopeptidase (M12 family) [So... [more]
Mep1b4.724e-1627.80meprin 1 beta [Source:MGI Symbol;Acc:MGI:96964][more]
Tll11.149e-1530.21tolloid-like [Source:MGI Symbol;Acc:MGI:106923][more]
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BLAST of Ast7 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P31579|LCE_ORYLA1.017e-1929.25Low choriolytic enzyme OS=Oryzias latipes OX=8090 ... [more]
sp|Q19269|NAS14_CAEEL2.937e-1828.42Zinc metalloproteinase nas-14 OS=Caenorhabditis el... [more]
sp|Q9U3S9|NAS6_CAEEL3.077e-1830.16Zinc metalloproteinase nas-6 OS=Caenorhabditis ele... [more]
sp|P55113|NAS7_CAEEL3.263e-1829.65Zinc metalloproteinase nas-7 OS=Caenorhabditis ele... [more]
sp|Q6HA08|ASTL_HUMAN3.997e-1828.44Astacin-like metalloendopeptidase OS=Homo sapiens ... [more]
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BLAST of Ast7 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A060Q7151.434e-17299.57Metalloendopeptidase (Fragment) OS=Schmidtea medit... [more]
A0A060Q6Y68.240e-13075.97Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7861.530e-4837.34Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7856.333e-4837.34Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7148.688e-4836.73Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
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BLAST of Ast7 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
c6ast38.856e-2227.60six-cysteine containing astacin protease 3 [Source... [more]
ENSAMXT00000038400.11.514e-2026.32pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000044667.12.325e-2026.32pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000021576.22.971e-2026.32pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
c6ast13.387e-2031.88low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
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BLAST of Ast7 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000007253.14.081e-1531.79pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1... [more]
ENSPMAT00000004913.12.584e-826.73pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 g... [more]
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BLAST of Ast7 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ast7 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO414206.708e-3333.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377704.145e-2831.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO348062.109e-2635.95Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO396273.633e-2632.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO424217.292e-2632.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ast7 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lce7.629e-2129.25protease [Source:NCBI gene;Acc:100049451][more]
lce1.135e-2029.25protease [Source:NCBI gene;Acc:100049451][more]
mc6ast23.491e-1929.80astacin like metallo-protease [Source:NCBI gene;Ac... [more]
mnep6.055e-1928.19nephrosin [Source:NCBI gene;Acc:100049315][more]
mnep8.266e-1928.32nephrosin [Source:NCBI gene;Acc:100049315][more]
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BLAST of Ast7 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000080475.12.355e-17699.57SMESG000080475.1[more]
SMESG000080471.12.291e-13375.97SMESG000080471.1[more]
SMESG000016662.16.385e-11665.38SMESG000016662.1[more]
SMESG000072791.14.254e-5237.34SMESG000072791.1[more]
SMESG000015174.11.761e-5137.34SMESG000015174.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30035521
GenBankCCW36845.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035521 ID=SMED30035521|Name=Ast7 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=809bp
GAAATTTCTATAAAGTTCGAATTTATAATTGAAAAAAAATATTAAAAATG
ATCTTGAAAATTGTAGTAATTTGTTTCTTACTAATATCTCTTTTTAGCTT
ATGCTATGCTCAATGTGGATGTACGCAGAAAGGAATTAAAATTGCTGACT
TCAATGTTGGAGACATGGTTCTTAGCCCTGTGGAAATCCTGAAAATAAAT
GGGTATAAAGCTGCTGATGGAGAGAAGTGGCCGAAAAATGAACTGCGATA
CACATTTGACCCATCTTTTCCTGATAAAAACAAAAACCCTGTCCGAGATT
TTCTTAAAGCATATGAAAAAGAATTGGGTAACTGTATTAAATTCAAAGAA
GAAAAAAGTGGCGTCTTTGCAACTGTTGTCACAGGAGGAGGTTGCTCTGC
TAATGTCGGATATAATTCACGGTATCCTCTAAAAGTTACGCTCGCCGATC
CTTGTGTTAATAAAAGAAACGTCAAACACGAATTCACCCATTCTCTTGGA
TTTCAACACGAACATACTCGACCAGATAGAGACAACAATTTAAACATCCA
GTTTAATAACGTAAAGAAAAATCTGTGTGCAAATTTTTATTTGTGCGAAG
GATGCAAAACTACAACTCCTTATGATTTTGACAGCATCATGCAATATCCT
TCAGAAGCTTTTACATGCAACGGTAAAGAAACGATATTAAGAAAAAATGG
TGGGAAAATAGCTTTCAATATGGAAGTAACAAAACTTGATGCTAAAAAAG
TACGAGACTATTATGGATGTGCCTAAAAGTTTTTTTTAAAATAGAAAGAT
TCATTAATT
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protein sequence of SMED30035521-orf-1

>SMED30035521-orf-1 ID=SMED30035521-orf-1|Name=SMED30035521-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=243bp
MILKIVVICFLLISLFSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKI
NGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFK
EEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSL
GFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQY
PSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGCA*
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Aliases
The feature 'Ast7 protein' has the following synonyms
Synonym
Ast7
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR001506Peptidase_M12A
IPR006026Peptidase_Metallo
IPR024079MetalloPept_cat_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008237metallopeptidase activity
GO:0008270zinc ion binding
GO:0008233peptidase activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001506Peptidase M12APRINTSPR00480ASTACINcoord: 155..172
score: 46.11
coord: 227..240
score: 21.9
coord: 136..154
score: 42.19
coord: 191..206
score: 47.71
IPR001506Peptidase M12APFAMPF01400Astacincoord: 60..241
e-value: 9.8E-35
score: 119.9
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 57..196
e-value: 5.5E-11
score: 52.5
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3DG3DSA:3.40.390.10coord: 25..242
e-value: 2.7E-47
score: 163.2
NoneNo IPR availablePANTHERPTHR10127DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAININGcoord: 38..241
NoneNo IPR availablePROSITEPS51864ASTACINcoord: 51..242
score: 31.469
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.947
NoneNo IPR availableSUPERFAMILYSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 57..241
NoneNo IPR availableTMHMMTMhelixcoord: 5..24