SMED30035493

Overview
NameSMED30035493
Smed IDSMED30035493
Length (bp)2173
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30035493 (SMED30035493) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30035493 (SMED30035493) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30035493 (SMED30035493) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30035493 (SMED30035493) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035493

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
muscle cellSMED30035493h1SMcG0012003 dd_Smed_v4_4495_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30035493h1SMcG0012003 dd_Smed_v4_4495_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30035493 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001306:21228..32256 +10696
Homology
[Close]
BLAST of SMED30035493 vs. Planmine SMEST
Match: SMESG000075822.1 (SMESG000075822.1)

HSP 1 Score: 1093.95 bits (2828), Expect = 0.000e+0
Identity = 623/627 (99.36%), Postives = 625/627 (99.68%), Query Frame = 1 
Query:   31 MSIKDSNFNIQISMSMNKSCDNLDYFGFTXXXXXXXXXXXXXXXXXQVREVTNCNEELAIINLHDCNYNVEYAINKILTHESSNEGEWXXXXXXXXXXXXXXXVLNKNQDQIKEKLQNSMESVTEVNESSDKKLLQFTKKENVCGKTFKNFNLTKRLNSNYIIPDHHRKNTLKPEINFLINGKWNEKTIIIENSKFPPMPVKDIIMLKNEKGESIDRSSPVQTYEKKENIEDVSIVFKTHCTTKPPPKVLCSLVAPVQIINNKTTKPHSLPKFKFGDTDFNEDIKKPDVTSKINNSDLKFVDIEDHSIIKVGPSQKIIHNQPICNFPSDIFNANPSYFNSPNFPMKTQSNLCNNNVKPEFNMKKPIDIAEYPYMNVLQHPGPTNLPXXXXXXXXXXXXXXXXXPKEINTNSKPHDMFLNHSSQLGYSPQTNVNHGKSTIYLVPNVMVNPISGLYMSVNNPLPTSALSPPMAPIASQFNMXXXXXXXMYYDESLQSDDIANKNHMVMRQYNQNRNFQRFKKNGPDITDEISIKTDHFSKPSMKTMPSIPMNQNSISKQIPYSFPLFISSASVASHNQSQMXXXXXXXXXXXXXXXXGFQPHQESNNNEHARKRNVQVQNGNFHGKAWS 1911
            MSIKDSNFNIQISMSMNKSCDNLDYFGFTDLIKKKLNEDELNLKLKQVREVTNCNEELAIINLHDCNYNVEYAINKILTHESSNEGEWKTKKGKQKKIIPKDKVLNKNQDQIKEK+QNSMESVTEVNESSDKKLLQFTKKENVCGKTFKNFNLTKRLNSNYIIPDHHRKNTLK EINFLINGKWNEKTIIIENSKFPPMPVKDIIMLKNEKGESIDRSSPVQTYEKKENIEDVSIVFKTHCTTKPPPKVLCSLVAPVQIINNKTTKPHSLPKFKFGDTDFNEDIKKPDVTSKINNSDLKFVDIEDHSIIKVGPSQKIIHNQPICNFPSDIFNANPSYFNSPNFPMKTQSNLCNNNVKPEFNMKKPIDIAEYPYMNVLQHPGPTNLPNCNSNMSSINSFNSSMSPKEINTNSKPHDMFLNHSSQLGYSPQTNVNHGKSTIYLVPNVMVNPISGLYMSVNNPLPTSALSPPMAPI+SQFNMPQQQPPPMYYDESLQSDDIANKNHM MRQYNQNRNFQRFKKNGPDITDEISIKTDHFSKPSMKTMPSIPMNQNSISKQIPYSFPLFISSASVASHNQSQMFINNPINSNSFSPFSNGFQPHQESNNNEHARKRNVQVQNGNFHGKAWS
Sbjct:    1 MSIKDSNFNIQISMSMNKSCDNLDYFGFTDLIKKKLNEDELNLKLKQVREVTNCNEELAIINLHDCNYNVEYAINKILTHESSNEGEWKTKKGKQKKIIPKDKVLNKNQDQIKEKIQNSMESVTEVNESSDKKLLQFTKKENVCGKTFKNFNLTKRLNSNYIIPDHHRKNTLKTEINFLINGKWNEKTIIIENSKFPPMPVKDIIMLKNEKGESIDRSSPVQTYEKKENIEDVSIVFKTHCTTKPPPKVLCSLVAPVQIINNKTTKPHSLPKFKFGDTDFNEDIKKPDVTSKINNSDLKFVDIEDHSIIKVGPSQKIIHNQPICNFPSDIFNANPSYFNSPNFPMKTQSNLCNNNVKPEFNMKKPIDIAEYPYMNVLQHPGPTNLPNCNSNMSSINSFNSSMSPKEINTNSKPHDMFLNHSSQLGYSPQTNVNHGKSTIYLVPNVMVNPISGLYMSVNNPLPTSALSPPMAPISSQFNMPQQQPPPMYYDESLQSDDIANKNHMAMRQYNQNRNFQRFKKNGPDITDEISIKTDHFSKPSMKTMPSIPMNQNSISKQIPYSFPLFISSASVASHNQSQMFINNPINSNSFSPFSNGFQPHQESNNNEHARKRNVQVQNGNFHGKAWS 627
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BLAST of SMED30035493 vs. Planmine SMEST
Match: SMESG000075822.1 (SMESG000075822.1)

HSP 1 Score: 1070.07 bits (2766), Expect = 0.000e+0
Identity = 610/614 (99.35%), Postives = 612/614 (99.67%), Query Frame = 1 
Query:   70 MSMNKSCDNLDYFGFTXXXXXXXXXXXXXXXXXQVREVTNCNEELAIINLHDCNYNVEYAINKILTHESSNEGEWXXXXXXXXXXXXXXXVLNKNQDQIKEKLQNSMESVTEVNESSDKKLLQFTKKENVCGKTFKNFNLTKRLNSNYIIPDHHRKNTLKPEINFLINGKWNEKTIIIENSKFPPMPVKDIIMLKNEKGESIDRSSPVQTYEKKENIEDVSIVFKTHCTTKPPPKVLCSLVAPVQIINNKTTKPHSLPKFKFGDTDFNEDIKKPDVTSKINNSDLKFVDIEDHSIIKVGPSQKIIHNQPICNFPSDIFNANPSYFNSPNFPMKTQSNLCNNNVKPEFNMKKPIDIAEYPYMNVLQHPGPTNLPXXXXXXXXXXXXXXXXXPKEINTNSKPHDMFLNHSSQLGYSPQTNVNHGKSTIYLVPNVMVNPISGLYMSVNNPLPTSALSPPMAPIASQFNMXXXXXXXMYYDESLQSDDIANKNHMVMRQYNQNRNFQRFKKNGPDITDEISIKTDHFSKPSMKTMPSIPMNQNSISKQIPYSFPLFISSASVASHNQSQMXXXXXXXXXXXXXXXXGFQPHQESNNNEHARKRNVQVQNGNFHGKAWS 1911
            MSMNKSCDNLDYFGFTDLIKKKLNEDELNLKLKQVREVTNCNEELAIINLHDCNYNVEYAINKILTHESSNEGEWKTKKGKQKKIIPKDKVLNKNQDQIKEK+QNSMESVTEVNESSDKKLLQFTKKENVCGKTFKNFNLTKRLNSNYIIPDHHRKNTLK EINFLINGKWNEKTIIIENSKFPPMPVKDIIMLKNEKGESIDRSSPVQTYEKKENIEDVSIVFKTHCTTKPPPKVLCSLVAPVQIINNKTTKPHSLPKFKFGDTDFNEDIKKPDVTSKINNSDLKFVDIEDHSIIKVGPSQKIIHNQPICNFPSDIFNANPSYFNSPNFPMKTQSNLCNNNVKPEFNMKKPIDIAEYPYMNVLQHPGPTNLPNCNSNMSSINSFNSSMSPKEINTNSKPHDMFLNHSSQLGYSPQTNVNHGKSTIYLVPNVMVNPISGLYMSVNNPLPTSALSPPMAPI+SQFNMPQQQPPPMYYDESLQSDDIANKNHM MRQYNQNRNFQRFKKNGPDITDEISIKTDHFSKPSMKTMPSIPMNQNSISKQIPYSFPLFISSASVASHNQSQMFINNPINSNSFSPFSNGFQPHQESNNNEHARKRNVQVQNGNFHGKAWS
Sbjct:    1 MSMNKSCDNLDYFGFTDLIKKKLNEDELNLKLKQVREVTNCNEELAIINLHDCNYNVEYAINKILTHESSNEGEWKTKKGKQKKIIPKDKVLNKNQDQIKEKIQNSMESVTEVNESSDKKLLQFTKKENVCGKTFKNFNLTKRLNSNYIIPDHHRKNTLKTEINFLINGKWNEKTIIIENSKFPPMPVKDIIMLKNEKGESIDRSSPVQTYEKKENIEDVSIVFKTHCTTKPPPKVLCSLVAPVQIINNKTTKPHSLPKFKFGDTDFNEDIKKPDVTSKINNSDLKFVDIEDHSIIKVGPSQKIIHNQPICNFPSDIFNANPSYFNSPNFPMKTQSNLCNNNVKPEFNMKKPIDIAEYPYMNVLQHPGPTNLPNCNSNMSSINSFNSSMSPKEINTNSKPHDMFLNHSSQLGYSPQTNVNHGKSTIYLVPNVMVNPISGLYMSVNNPLPTSALSPPMAPISSQFNMPQQQPPPMYYDESLQSDDIANKNHMAMRQYNQNRNFQRFKKNGPDITDEISIKTDHFSKPSMKTMPSIPMNQNSISKQIPYSFPLFISSASVASHNQSQMFINNPINSNSFSPFSNGFQPHQESNNNEHARKRNVQVQNGNFHGKAWS 614
[Close]
BLAST of SMED30035493 vs. Planmine SMEST
Match: SMESG000075822.1 (SMESG000075822.1)

HSP 1 Score: 1065.83 bits (2755), Expect = 0.000e+0
Identity = 608/612 (99.35%), Postives = 610/612 (99.67%), Query Frame = 1 
Query:   76 MNKSCDNLDYFGFTXXXXXXXXXXXXXXXXXQVREVTNCNEELAIINLHDCNYNVEYAINKILTHESSNEGEWXXXXXXXXXXXXXXXVLNKNQDQIKEKLQNSMESVTEVNESSDKKLLQFTKKENVCGKTFKNFNLTKRLNSNYIIPDHHRKNTLKPEINFLINGKWNEKTIIIENSKFPPMPVKDIIMLKNEKGESIDRSSPVQTYEKKENIEDVSIVFKTHCTTKPPPKVLCSLVAPVQIINNKTTKPHSLPKFKFGDTDFNEDIKKPDVTSKINNSDLKFVDIEDHSIIKVGPSQKIIHNQPICNFPSDIFNANPSYFNSPNFPMKTQSNLCNNNVKPEFNMKKPIDIAEYPYMNVLQHPGPTNLPXXXXXXXXXXXXXXXXXPKEINTNSKPHDMFLNHSSQLGYSPQTNVNHGKSTIYLVPNVMVNPISGLYMSVNNPLPTSALSPPMAPIASQFNMXXXXXXXMYYDESLQSDDIANKNHMVMRQYNQNRNFQRFKKNGPDITDEISIKTDHFSKPSMKTMPSIPMNQNSISKQIPYSFPLFISSASVASHNQSQMXXXXXXXXXXXXXXXXGFQPHQESNNNEHARKRNVQVQNGNFHGKAWS 1911
            MNKSCDNLDYFGFTDLIKKKLNEDELNLKLKQVREVTNCNEELAIINLHDCNYNVEYAINKILTHESSNEGEWKTKKGKQKKIIPKDKVLNKNQDQIKEK+QNSMESVTEVNESSDKKLLQFTKKENVCGKTFKNFNLTKRLNSNYIIPDHHRKNTLK EINFLINGKWNEKTIIIENSKFPPMPVKDIIMLKNEKGESIDRSSPVQTYEKKENIEDVSIVFKTHCTTKPPPKVLCSLVAPVQIINNKTTKPHSLPKFKFGDTDFNEDIKKPDVTSKINNSDLKFVDIEDHSIIKVGPSQKIIHNQPICNFPSDIFNANPSYFNSPNFPMKTQSNLCNNNVKPEFNMKKPIDIAEYPYMNVLQHPGPTNLPNCNSNMSSINSFNSSMSPKEINTNSKPHDMFLNHSSQLGYSPQTNVNHGKSTIYLVPNVMVNPISGLYMSVNNPLPTSALSPPMAPI+SQFNMPQQQPPPMYYDESLQSDDIANKNHM MRQYNQNRNFQRFKKNGPDITDEISIKTDHFSKPSMKTMPSIPMNQNSISKQIPYSFPLFISSASVASHNQSQMFINNPINSNSFSPFSNGFQPHQESNNNEHARKRNVQVQNGNFHGKAWS
Sbjct:    1 MNKSCDNLDYFGFTDLIKKKLNEDELNLKLKQVREVTNCNEELAIINLHDCNYNVEYAINKILTHESSNEGEWKTKKGKQKKIIPKDKVLNKNQDQIKEKIQNSMESVTEVNESSDKKLLQFTKKENVCGKTFKNFNLTKRLNSNYIIPDHHRKNTLKTEINFLINGKWNEKTIIIENSKFPPMPVKDIIMLKNEKGESIDRSSPVQTYEKKENIEDVSIVFKTHCTTKPPPKVLCSLVAPVQIINNKTTKPHSLPKFKFGDTDFNEDIKKPDVTSKINNSDLKFVDIEDHSIIKVGPSQKIIHNQPICNFPSDIFNANPSYFNSPNFPMKTQSNLCNNNVKPEFNMKKPIDIAEYPYMNVLQHPGPTNLPNCNSNMSSINSFNSSMSPKEINTNSKPHDMFLNHSSQLGYSPQTNVNHGKSTIYLVPNVMVNPISGLYMSVNNPLPTSALSPPMAPISSQFNMPQQQPPPMYYDESLQSDDIANKNHMAMRQYNQNRNFQRFKKNGPDITDEISIKTDHFSKPSMKTMPSIPMNQNSISKQIPYSFPLFISSASVASHNQSQMFINNPINSNSFSPFSNGFQPHQESNNNEHARKRNVQVQNGNFHGKAWS 612
The following BLAST results are available for this feature:
BLAST of SMED30035493 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035493 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000075822.10.000e+099.36SMESG000075822.1[more]
SMESG000075822.10.000e+099.35SMESG000075822.1[more]
SMESG000075822.10.000e+099.35SMESG000075822.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035493 ID=SMED30035493|Name=SMED30035493|organism=Schmidtea mediterranea sexual|type=transcript|length=2173bp
GTTTTATTTAAGTAAACTTTTGTATATCAAATGTCAATAAAAGATAGCAA
TTTTAATATACAAATTTCTATGAGTATGAATAAAAGTTGTGATAATTTAG
ATTATTTTGGTTTTACAGATCTTATCAAAAAGAAATTAAACGAAGATGAA
CTTAATTTAAAATTAAAACAAGTTCGTGAAGTGACTAATTGTAATGAAGA
ACTTGCAATTATAAACCTTCATGATTGTAATTATAATGTTGAGTACGCAA
TTAATAAAATATTAACTCATGAATCATCAAATGAAGGAGAGTGGAAAACT
AAAAAAGGGAAACAAAAGAAAATAATACCAAAAGATAAAGTTCTTAATAA
AAATCAAGATCAAATCAAGGAAAAACTTCAAAATTCTATGGAGTCAGTTA
CAGAAGTAAATGAAAGTTCTGATAAAAAATTATTACAATTTACAAAAAAA
GAAAATGTTTGTGGTAAAACGTTTAAAAATTTTAATTTGACCAAGAGACT
AAACTCTAATTATATAATTCCCGATCATCACAGAAAAAATACTTTGAAAC
CAGAAATAAATTTTTTGATAAATGGAAAATGGAATGAGAAAACAATTATT
ATTGAAAATTCAAAATTCCCACCTATGCCTGTCAAGGATATTATTATGCT
AAAAAATGAAAAAGGTGAAAGTATCGACAGAAGTTCACCGGTACAAACTT
ATGAGAAAAAAGAAAACATTGAAGACGTATCTATTGTTTTTAAAACACAC
TGCACAACAAAACCGCCGCCCAAAGTGTTGTGTTCTCTAGTGGCACCGGT
ACAAATAATTAATAATAAAACCACTAAGCCACATTCTCTGCCGAAATTCA
AATTCGGAGATACTGATTTCAATGAAGACATTAAGAAGCCTGATGTTACT
AGTAAAATAAATAATTCCGATTTGAAATTTGTCGATATCGAGGATCATTC
GATAATTAAAGTTGGGCCCTCACAGAAGATAATACACAATCAACCGATCT
GTAACTTTCCATCCGATATCTTTAATGCAAACCCCTCGTATTTCAATTCT
CCGAATTTCCCAATGAAAACCCAATCTAACTTGTGCAACAACAATGTGAA
ACCCGAATTCAATATGAAAAAACCCATTGATATCGCCGAGTATCCATACA
TGAATGTGTTACAACACCCTGGACCAACAAATCTCCCGAATTGCAATAGC
AATATGTCATCGATCAATTCTTTCAATTCTTCAATGTCGCCTAAAGAAAT
AAATACCAACTCAAAACCACATGATATGTTTCTTAATCATTCGTCTCAAC
TCGGTTACTCGCCACAAACTAACGTCAATCATGGCAAATCCACCATTTAT
CTAGTGCCGAATGTGATGGTGAATCCAATAAGTGGATTATATATGTCTGT
CAACAATCCCCTACCAACAAGTGCCCTTAGTCCACCAATGGCTCCTATTG
CATCACAGTTTAATATGCCGCAACAGCAACCACCTCCTATGTATTACGAC
GAATCTCTTCAGTCTGATGACATAGCCAATAAAAATCACATGGTCATGAG
ACAATACAATCAAAACCGGAATTTCCAGAGATTCAAAAAGAATGGTCCTG
ATATCACGGATGAAATCTCAATTAAAACTGACCATTTTTCAAAACCCAGC
ATGAAAACTATGCCGAGTATTCCTATGAATCAAAATTCTATTTCGAAACA
AATCCCTTACAGCTTTCCTTTATTCATTTCATCAGCATCAGTGGCGTCTC
ACAACCAATCCCAAATGTTTATAAACAACCCAATCAATTCTAACTCTTTC
AGTCCTTTCTCCAATGGTTTCCAGCCTCACCAGGAATCAAACAACAATGA
ACATGCGAGGAAAAGAAATGTTCAAGTACAGAATGGGAACTTCCATGGTA
AAGCATGGTCATAATGTTTAAGATTGAACATTTTGTTTGTTAAAACTGAA
TTTATATTTAGTGTGTAAATTTAGATCAATTATTTGACTTTCATTAATAG
ACAGCTTAAATCTTATTTATACATATATATATTTATTGGTTAATACTGAA
TTGGCCGTATGATTGGTTGATTTCAAATTTTTAATTTACACATGACATAG
AATTCAGTAAAATATAGGTCAAGTGATGTTTGTTATCAAAATTGCTATCC
AAATTATGGACTATATTGTAGAG
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protein sequence of SMED30035493-orf-1

>SMED30035493-orf-1 ID=SMED30035493-orf-1|Name=SMED30035493-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=628bp
MSIKDSNFNIQISMSMNKSCDNLDYFGFTDLIKKKLNEDELNLKLKQVRE
VTNCNEELAIINLHDCNYNVEYAINKILTHESSNEGEWKTKKGKQKKIIP
KDKVLNKNQDQIKEKLQNSMESVTEVNESSDKKLLQFTKKENVCGKTFKN
FNLTKRLNSNYIIPDHHRKNTLKPEINFLINGKWNEKTIIIENSKFPPMP
VKDIIMLKNEKGESIDRSSPVQTYEKKENIEDVSIVFKTHCTTKPPPKVL
CSLVAPVQIINNKTTKPHSLPKFKFGDTDFNEDIKKPDVTSKINNSDLKF
VDIEDHSIIKVGPSQKIIHNQPICNFPSDIFNANPSYFNSPNFPMKTQSN
LCNNNVKPEFNMKKPIDIAEYPYMNVLQHPGPTNLPNCNSNMSSINSFNS
SMSPKEINTNSKPHDMFLNHSSQLGYSPQTNVNHGKSTIYLVPNVMVNPI
SGLYMSVNNPLPTSALSPPMAPIASQFNMPQQQPPPMYYDESLQSDDIAN
KNHMVMRQYNQNRNFQRFKKNGPDITDEISIKTDHFSKPSMKTMPSIPMN
QNSISKQIPYSFPLFISSASVASHNQSQMFINNPINSNSFSPFSNGFQPH
QESNNNEHARKRNVQVQNGNFHGKAWS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR009060UBA-like_sf
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.10.8.10coord: 28..79
e-value: 4.2E-9
score: 38.3
NoneNo IPR availableCDDcd14277UBA_UBP2_likecoord: 41..78
e-value: 5.85882E-11
score: 55.6453
IPR009060UBA-like superfamilySUPERFAMILYSSF46934UBA-likecoord: 37..97