SMED30035488

Overview
NameSMED30035488
Smed IDSMED30035488
Length (bp)2093
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30035488 (SMED30035488) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30035488 (SMED30035488) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30035488 (SMED30035488) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30035488 (SMED30035488) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035488

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30035488h1SMcG0006563 Contig3349GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30035488h1SMcG0006567 Contig6905uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30035488h1SMcG0006567 Contig6905newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30035488h1SMcG0006563 Contig907newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30035488h1SMcG0009497 Contig907newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30035488h1SMcG0006563 Contig55219uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30035488h1SMcG0006563 Contig55219newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30035488h1SMcG0006563 dd_Smed_v4_11479_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30035488h1SMcG0006568 dd_Smed_v4_5607_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30035488h1SMcG0006568 dd_Smed_v4_5607_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30035488h1SMcG0006568 dd_Smed_v4_5607_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30035488h1SMcG0006568 dd_Smed_v6_5607_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
oocyteSMED30035488h1SMcG0006568 Contig52462newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30035488h1SMcG0006568 Contig52462uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30035488h1SMcG0006568 Contig52462newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30035488h1SMcG0006568 Contig52462uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30035488h1SMcG0006568 Contig52462newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30035488h1SMcG0006568 Contig52462uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30035488h1SMcG0006568 dd_Smed_v4_5607_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30035488 vs. TrEMBL
Match: A0A2T7PUB9 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_04002 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.865e-15
Identity = 56/204 (27.45%), Postives = 97/204 (47.55%), Query Frame = 3 
Query:  756 CLSISQLMCEIRSRVYPFIINKNLTSVTE---SMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSL--NFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFY 1352
            C    +L+  +R+ +Y  ++ ++   VTE   +M  D + M    V    +        P+    R+N +   + +F  I + LE   +    +  K+G + GF+ Y+LRY   +   + +N+ E EF A+ A AF  S  ++        +++CP  RCL I + F  IY     F GS  +L  +FPLP+++ S S +T  Y
Sbjct:  381 CAPAEELLAPLRADLYAILLPRHKAFVTEYGQTMRQDFQQMQNYAVNDKNL--------PSLNHIRENQIFRNMRLFHYIMTRLEPGQAPEKTWFDKYGRKTGFIFYILRYFLLLNWGQCLNVTEAEFLALVATAFGASKESSWQE-----LRVCPDPRCLTIAALFQDIYRHAFLFLGSVLHLKHEFPLPKEIFSGSMWTVLY 571
[Close]
BLAST of SMED30035488 vs. TrEMBL
Match: K1QJE9 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10014990 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.689e-15
Identity = 66/274 (24.09%), Postives = 124/274 (45.26%), Query Frame = 3 
Query:  531 NFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFY 1352
            +F  AVE    FY ++   E            +  G  +    +K+S ++    L   H   +   +L E+ T       + + E+R+ +Y  ++ +    V E  +G S     +++E      D  +  PA    +++ + + ++ F  I SH E      +  ++G + GF+ Y LRY   +   +N+ L ++EF A+ A+ F++        +    ++I P+ RC+ I +WF+ +Y     F G    L  +FPLPE++ S S +T FY
Sbjct:  315 SFARAVEHSRMFYCLQSNPEEI----------VHKGYFSKLLAEKISSLKYPSHLMAMHNNFYWHRLLQEDTTRGQPCAEEALTELRALIYRIVLPRRENLVNE--YGRS-PWDTLRIEGVLAIDDPNL--PALHKIQEDKIFWNLNSFHYIMSHQEPVERPKWFDRYGRKNGFIVYCLRYFLLLNWGRNLQLQQQEFLALCALVFARP-----REEHYQQIQIRPTPRCVSIGNWFLDVYRHAFLFLGKLLYLTHEFPLPEEIYSGSVWTCFY 568
[Close]
BLAST of SMED30035488 vs. TrEMBL
Match: A0A0B7A3T0 (Uncharacterized protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF91910 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.083e-13
Identity = 57/195 (29.23%), Postives = 93/195 (47.69%), Query Frame = 3 
Query:  783 EIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENE---NSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYET 1358
            ++R+ +Y  I+  +  SVTE     S  + EI V  S +    I   P     +Q+     + +FE I  H E++      ++   +GL KGF+C +LRY   +   KN+ L  +EF  V A       R T+ +     M I PS RC+ + +WF+ +Y     F G+  +L  +FP P D+ S + + A Y +
Sbjct:   12 DLRAYIYSMILPPHKMSVTEIGRTPSEPVKEIVV--SKVVDPNI---PDLVQVKQSSFSQKLHVFEVIMQHQESQVFNEERSYFKMYGLRKGFICCLLRYFLLMNWGKNLFLSAQEFFVVIAWMLGN--RDTRYQQ----MSIVPSPRCITVENWFLTLYRNAYAFLGNILSLSNEFPAPRDLFSGATWVALYTS 195
[Close]
BLAST of SMED30035488 vs. TrEMBL
Match: R7ULZ5 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_190174 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.928e-11
Identity = 39/131 (29.77%), Postives = 65/131 (49.62%), Query Frame = 3 
Query:  975 FEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMN 1367
            F +I++H E E   N+ + +G+   F CYVLRY   +   +N+ +   EF A   M F K      + +  +   + P+ RCL + +W   +Y       G   +L  +F  P+DVIS S + AFY  +++
Sbjct:  420 FAKIFTHFEPECLPNWGANYGMNDAFRCYVLRYFLVLNTGRNLRMDYSEFTATAGMFF-KQTAGAMDEEWYIKQALVPNRRCLTMLNWLQTLYRYAYVMIGKLLHLCSEFSEPKDVISGSIWVAFYAVSVH 549
[Close]
BLAST of SMED30035488 vs. TrEMBL
Match: A0A210QAR5 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT01511 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.211e-11
Identity = 65/292 (22.26%), Postives = 125/292 (42.81%), Query Frame = 3 
Query:  498 VFVQKLKKKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDS-----RNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSL--NFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFY 1352
            VF Q+++     F+ AV    NFY +       G   +K  +    G L+   +K + +   +  +   H   +    + E+ +         +  +R+ VY  ++ ++ + V E  +G S     R    + VE S I        P+    + + + + +  F  I SH+E        +  ++G + GF  Y+LRY   +   +N+ + E EF A+ AM+  +            G+ + P+ RC+ + SW+  +Y+    F G   ++  +FPLP +  S + +T FY
Sbjct:  303 VFAQEMRHNM-GFRGAVVHSRNFYNL-------GVPPLKPSKT---GHLSQILEKGIREGHFVARVMGMHNNFYWHRAVVEDLSEGQPCAEVALAPLRAHVYRIVLQRHESMVEE--YGRSPWEPMRTAGVLAVEDSNI--------PSIHRIQPDKIFWNLKHFHYIMSHMERRTGSESKWFERYGRKTGFSVYLLRYFLLLNWGRNLFVTENEFLALLAMSLGRPSAAKYQ-----GICVRPTPRCVTLGSWYQDLYYHAYCFLGKLLSISHEFPLPCEAYSGAVWTVFY 568
[Close]
BLAST of SMED30035488 vs. Planmine SMEST
Match: SMESG000033468.1 (SMESG000033468.1)

HSP 1 Score: 962.6 bits (2487), Expect = 0.000e+0
Identity = 470/473 (99.37%), Postives = 472/473 (99.79%), Query Frame = 3 
Query:   57 MGVQQLYSYISQNAIKVVDLIQEAQKSNPMEILVDFNNFRRCLDEIILLKIKNDYENEYLATFCFEYDIFTNAYCEFHEMFAEMGIKLVWIVDGIKGIDMEVNKHKYETWSGRDSKYTEFVNSIFKSCRENIPIGKCVNYSQFYEKMVFVQKLKKKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMNDKNKRVPDSNEIIINGLEMNLMKDFEAEYQVVTKYD 1475
            MGVQQLYSYISQNAIKVVDLIQEAQKSNPMEILVDFNNFRRCLDEIILLKIKNDYENEYLATFCFEYDIFTNAYCEFHEMFAEMGIKLVWIVDGIKGIDMEVNKHKYETWSGRDSKYTEFVNSIFKSCRENIPIGKCVNYSQFYEKMVFVQKLKKKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMNDKNKRVPDSNEIII GLEM+LMKDFEAEY+VVTKYD
Sbjct:    1 MGVQQLYSYISQNAIKVVDLIQEAQKSNPMEILVDFNNFRRCLDEIILLKIKNDYENEYLATFCFEYDIFTNAYCEFHEMFAEMGIKLVWIVDGIKGIDMEVNKHKYETWSGRDSKYTEFVNSIFKSCRENIPIGKCVNYSQFYEKMVFVQKLKKKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMNDKNKRVPDSNEIIIYGLEMDLMKDFEAEYKVVTKYD 473
[Close]
BLAST of SMED30035488 vs. Planmine SMEST
Match: SMESG000033468.1 (SMESG000033468.1)

HSP 1 Score: 643.269 bits (1658), Expect = 0.000e+0
Identity = 317/324 (97.84%), Postives = 320/324 (98.77%), Query Frame = 3 
Query:  504 VQKLKKKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMNDKNKRVPDSNEIIINGLEMNLMKDFEAEYQVVTKYD 1475
            + K  KKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMNDKNKRVPDSNEIII GLEM+LMKDFEAEY+VVTKYD
Sbjct:    5 MNKYLKKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMNDKNKRVPDSNEIIIYGLEMDLMKDFEAEYKVVTKYD 328
[Close]
BLAST of SMED30035488 vs. Planmine SMEST
Match: SMESG000033479.1 (SMESG000033479.1)

HSP 1 Score: 348.591 bits (893), Expect = 7.171e-111
Identity = 172/324 (53.09%), Postives = 224/324 (69.14%), Query Frame = 3 
Query:  528 PNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSK-------SIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMNDKNKRVPDSNEII---INGLEMNLMKDFEAEYQVVTK 1469
            P FK  V FCF+FYT++H   +SGK LI FD   +DGSLTSF Q+KLS +EI D LY+ HFG++      E+P  +  +I+ +M EIRSR+YP++++ +L  VTE    D+  ++ I V  S  KS+ II KP  ESERQNGV YGIDIFEEIWSHLE+++SLNFV +FGLEKGFLCY+LRY+YK+T   NI +  +EF A+FAM F +         RT  N+D+ LGMKICP+E+CL IFSWFM+IY T  YFCG A NLL DFP  E+V+SS+ F AFYE N+ND NKRV   +E +   I  LE  +++  +  YQ +TK
Sbjct:  290 PEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGIL-INVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAITK 612

HSP 2 Score: 137.117 bits (344), Expect = 1.259e-33
Identity = 74/176 (42.05%), Postives = 109/176 (61.93%), Query Frame = 3 
Query:  144 MEILVDFNNFRRCLDEIILLKIKNDYENEYLATFCFEYDIFTNAYCEFHEMFAEMGIKLVWIVDGIKGIDMEVNKHKYETWSGRDSKYTEFVNSIFKSCRENIPIGKCVNYSQFYEKMVFVQKLKKKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLS 671
            MEILVDF++FRR LDEIIL KI ND++N++L+  C E DIF+ AY EFH MFAE+GIKLVWI DGIKG+D  V+  K ETWS R +KY+  V+++ + C+  +P+                        NFK+ + + ++   +++L ++SG  +IK  +  SD  + S F ++ S
Sbjct:    1 MEILVDFSSFRRGLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNSVDAVLECCKNKMPV-----------------------KNFKDVISWAYSLTALENL-KASGVEVIKLLQGGSDEFIASEFHRRQS 152
[Close]
BLAST of SMED30035488 vs. Planmine SMEST
Match: SMESG000033479.1 (SMESG000033479.1)

HSP 1 Score: 343.969 bits (881), Expect = 3.019e-109
Identity = 173/324 (53.40%), Postives = 222/324 (68.52%), Query Frame = 3 
Query:  528 PNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSK-------SIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMNDKNKRVPDSNEII---INGLEMNLMKDFEAEYQVVTK 1469
            P FK  V FCF+FYT++H   +SGK LI FD   +DGSLTSF Q+KLS +EI D LY+ HFG++      E+P  +  +I+ +M EIRSR+YP++++ +L  VTE    D+  ++ I V  S  KS+ II KP  ESERQNGV YGIDIFEEIWSHL  ENSLNFV +FGLEKGFLCY+LRY+YK+T   NI +  +EF A+FAM F +         RT  N+D+ LGMKICP+E+CL IFSWFM+IY T  YFCG A NLL DFP  E+V+SS+ F AFYE N+ND NKRV   +E +   I  LE  +++  +  YQ +TK
Sbjct:  290 PEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGIL-INVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHL--ENSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAITK 610

HSP 2 Score: 137.117 bits (344), Expect = 1.310e-33
Identity = 74/176 (42.05%), Postives = 109/176 (61.93%), Query Frame = 3 
Query:  144 MEILVDFNNFRRCLDEIILLKIKNDYENEYLATFCFEYDIFTNAYCEFHEMFAEMGIKLVWIVDGIKGIDMEVNKHKYETWSGRDSKYTEFVNSIFKSCRENIPIGKCVNYSQFYEKMVFVQKLKKKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLS 671
            MEILVDF++FRR LDEIIL KI ND++N++L+  C E DIF+ AY EFH MFAE+GIKLVWI DGIKG+D  V+  K ETWS R +KY+  V+++ + C+  +P+                        NFK+ + + ++   +++L ++SG  +IK  +  SD  + S F ++ S
Sbjct:    1 MEILVDFSSFRRGLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNSVDAVLECCKNKMPV-----------------------KNFKDVISWAYSLTALENL-KASGVEVIKLLQGGSDEFIASEFHRRQS 152
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BLAST of SMED30035488 vs. Planmine SMEST
Match: SMESG000033479.1 (SMESG000033479.1)

HSP 1 Score: 348.977 bits (894), Expect = 8.650e-109
Identity = 172/324 (53.09%), Postives = 224/324 (69.14%), Query Frame = 3 
Query:  528 PNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYPFIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGVVYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNINLFEEEFKAVFAMAFSK-------SIRTTKNRDLILGMKICPSERCLIIFSWFMKIYFTGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMNDKNKRVPDSNEII---INGLEMNLMKDFEAEYQVVTK 1469
            P FK  V FCF+FYT++H   +SGK LI FD   +DGSLTSF Q+KLS +EI D LY+ HFG++      E+P  +  +I+ +M EIRSR+YP++++ +L  VTE    D+  ++ I V  S  KS+ II KP  ESERQNGV YGIDIFEEIWSHLE+++SLNFV +FGLEKGFLCY+LRY+YK+T   NI +  +EF A+FAM F +         RT  N+D+ LGMKICP+E+CL IFSWFM+IY T  YFCG A NLL DFP  E+V+SS+ F AFYE N+ND NKRV   +E +   I  LE  +++  +  YQ +TK
Sbjct:  496 PEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGIL-INVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAITK 818

HSP 2 Score: 137.502 bits (345), Expect = 2.274e-33
Identity = 74/176 (42.05%), Postives = 109/176 (61.93%), Query Frame = 3 
Query:  144 MEILVDFNNFRRCLDEIILLKIKNDYENEYLATFCFEYDIFTNAYCEFHEMFAEMGIKLVWIVDGIKGIDMEVNKHKYETWSGRDSKYTEFVNSIFKSCRENIPIGKCVNYSQFYEKMVFVQKLKKKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFFQKKLS 671
            MEILVDF++FRR LDEIIL KI ND++N++L+  C E DIF+ AY EFH MFAE+GIKLVWI DGIKG+D  V+  K ETWS R +KY+  V+++ + C+  +P+                        NFK+ + + ++   +++L ++SG  +IK  +  SD  + S F ++ S
Sbjct:    1 MEILVDFSSFRRGLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNSVDAVLECCKNKMPV-----------------------KNFKDVISWAYSLTALENL-KASGVEVIKLLQGGSDEFIASEFHRRQS 152
The following BLAST results are available for this feature:
BLAST of SMED30035488 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2T7PUB91.865e-1527.45Uncharacterized protein OS=Pomacea canaliculata OX... [more]
K1QJE92.689e-1524.09Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A0B7A3T01.083e-1329.23Uncharacterized protein (Fragment) OS=Arion vulgar... [more]
R7ULZ52.928e-1129.77Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A210QAR53.211e-1122.26Uncharacterized protein OS=Mizuhopecten yessoensis... [more]
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BLAST of SMED30035488 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035488 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000033468.10.000e+099.37SMESG000033468.1[more]
SMESG000033468.10.000e+097.84SMESG000033468.1[more]
SMESG000033479.17.171e-11153.09SMESG000033479.1[more]
SMESG000033479.13.019e-10953.40SMESG000033479.1[more]
SMESG000033479.18.650e-10953.09SMESG000033479.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035488 ID=SMED30035488|Name=SMED30035488|organism=Schmidtea mediterranea sexual|type=transcript|length=2093bp
CAAGTGTTTCCTAAAACTTAAATCAAAGAAATTTTATAATATTTTAATAG
TTTGAAATGGGTGTACAGCAACTCTATTCATACATTTCTCAGAATGCCAT
TAAAGTTGTGGATTTAATTCAAGAAGCTCAGAAGTCAAATCCCATGGAAA
TTTTAGTAGATTTTAACAATTTTCGGAGATGTCTTGACGAAATTATTTTG
CTGAAAATCAAAAATGACTACGAAAACGAATATTTAGCTACGTTTTGCTT
TGAATATGATATTTTCACCAATGCCTATTGTGAATTTCACGAAATGTTTG
CGGAAATGGGTATTAAATTGGTTTGGATTGTAGATGGAATAAAGGGCATT
GATATGGAGGTGAATAAGCACAAGTATGAGACATGGTCCGGTCGGGATAG
TAAATATACAGAATTTGTTAATTCAATTTTTAAAAGTTGTCGGGAAAATA
TACCTATTGGCAAATGTGTGAATTATTCTCAGTTTTATGAGAAAATGGTG
TTTGTTCAGAAATTGAAAAAGAAATATCCAAATTTCAAAGAGGCAGTTGA
ATTTTGCTTTAATTTTTATACTATGAAACATTTAAGTGAAAGTTCTGGAA
AGAATTTAATAAAATTTGACCGAAAAATGAGTGATGGCTCGCTTACAAGT
TTTTTTCAAAAAAAATTATCCGACGTTGAAATCATTGATGAATTATATGT
GGGACATTTTGGAAAATTCGATATAACTATCTTGTTTGAAGAACCAACAG
GATCCTGTCTATCCATATCTCAATTAATGTGTGAGATACGTTCAAGGGTT
TATCCATTTATTATTAATAAAAACCTTACAAGTGTAACTGAATCGATGCA
TGGAGATTCACGAAATATGATGGAAATAAAAGTTGAAACCTCCGCAATAA
AATCAGACCGTATTATAGGTAAGCCAGCTTCGGAATCGGAACGGCAAAAC
GGAGTTGTATACGGAATTGATATATTTGAAGAAATATGGTCACATTTAGA
AAATGAGAATTCATTGAATTTCGTAAGCAAATTTGGATTGGAAAAAGGAT
TTCTCTGTTACGTTTTGAGATATATGTATAAAATAACAAAAAATAAAAAT
ATCAATTTGTTTGAAGAGGAATTTAAAGCGGTATTTGCAATGGCCTTCTC
TAAATCAATCCGTACAACTAAAAATCGAGATTTGATTTTGGGAATGAAAA
TCTGTCCTTCAGAAAGGTGCTTGATAATATTTTCTTGGTTTATGAAAATA
TACTTTACCGGGATATATTTCTGTGGAAGTGCATTCAATTTGTTATGTGA
TTTTCCGTTGCCGGAAGACGTTATTAGTTCAAGTTTTTTTACCGCGTTCT
ATGAAACCAACATGAATGATAAAAACAAGAGAGTTCCAGATTCAAATGAA
ATTATAATTAACGGCTTAGAAATGAATTTAATGAAAGATTTCGAAGCCGA
ATATCAAGTTGTCACAAAATATGACTAATGAAAACAATTAAAACTTATAT
CTAACTAATTTATGCAAATTTTACATTATTTTTTATTTGTTTAGGGAGTG
GGTTCTCTAATTTATTTGTTTTTCTGATTTTTCATTTGTTAGCTTTCATC
GGAATTCTGTGATTATTTATTATTTTTAGTATTGGGAGTCACTGATAAAT
GATGCCATTTAGTTTTAGCCTCTCTATAGTCACAAAAAGCAATACTCTCA
AATATAATGCTGATGTCATGGGTCTAAAATTAATTTGTTTAAATTAGCCT
TAATTACTTGAATTATCTTTCTCGGTCGTGACACTTCCTTTTCTTGGGGT
TTATACTATCTCACCCGGACGCCCCGCAATTTTTATCTTACCACCATGGA
CCCATATTGGTACCAAGTATAGCAGCCGAGAATCTCACAAGCATGTGAGC
GTCTACCTTAGCACTACTTCTTTTGCGATTCTTGCTTACGGGTTCTTGAC
TGTTGGTATAAATGCATACGTCATTCTCTTCTTCAATATTACCTCCCTTT
CGTTTAATTACTCTGCTAACTTGGATCCATGCAGCCATAGTAAGTATTAT
GTATACACCCTGTATTACATTCGGAATTTATCCTAAATATACC
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protein sequence of SMED30035488-orf-1

>SMED30035488-orf-1 ID=SMED30035488-orf-1|Name=SMED30035488-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=474bp
MGVQQLYSYISQNAIKVVDLIQEAQKSNPMEILVDFNNFRRCLDEIILLK
IKNDYENEYLATFCFEYDIFTNAYCEFHEMFAEMGIKLVWIVDGIKGIDM
EVNKHKYETWSGRDSKYTEFVNSIFKSCRENIPIGKCVNYSQFYEKMVFV
QKLKKKYPNFKEAVEFCFNFYTMKHLSESSGKNLIKFDRKMSDGSLTSFF
QKKLSDVEIIDELYVGHFGKFDITILFEEPTGSCLSISQLMCEIRSRVYP
FIINKNLTSVTESMHGDSRNMMEIKVETSAIKSDRIIGKPASESERQNGV
VYGIDIFEEIWSHLENENSLNFVSKFGLEKGFLCYVLRYMYKITKNKNIN
LFEEEFKAVFAMAFSKSIRTTKNRDLILGMKICPSERCLIIFSWFMKIYF
TGIYFCGSAFNLLCDFPLPEDVISSSFFTAFYETNMNDKNKRVPDSNEII
INGLEMNLMKDFEAEYQVVTKYD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron