phenylalanine--tRNA ligase alpha subunit-like

Overview
Namephenylalanine--tRNA ligase alpha subunit-like
Smed IDSMED30035483
Length (bp)1570
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of phenylalanine--tRNA ligase alpha subunit-like (SMED30035483) t-SNE clustered cells

Violin plots show distribution of expression levels for phenylalanine--tRNA ligase alpha subunit-like (SMED30035483) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of phenylalanine--tRNA ligase alpha subunit-like (SMED30035483) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for phenylalanine--tRNA ligase alpha subunit-like (SMED30035483) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035483

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30035483h1SMcG0009631 dd_Smed_v4_3224_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30035483h1SMcG0009631 dd_Smed_v4_3224_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30035483h1SMcG0009631 dd_Smed_v4_3224_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Human
Match: FARSA (phenylalanyl-tRNA synthetase subunit alpha [Source:HGNC Symbol;Acc:HGNC:3592])

HSP 1 Score: 466.848 bits (1200), Expect = 3.182e-160
Identity = 212/346 (61.27%), Postives = 278/346 (80.35%), Query Frame = 2 
Query:  449 GDQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYN-LHGGIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            G++  ++ +KRKL+ +VT+K + + KG  FST+  +     ++ E I    +    FKPYN L  G++    +LHPLLK+R ++++I +E+GF EMPT+N++ESSFWNFDALFQPQ H ARD HDTF+L +P ++ Q P +Y++RV   HS+GGYGSQGY+  WKL+EARKNLLRTHTT+ SAR LYRL+Q+K FTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++AD GL+L +L   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP +V+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +P+CRLD
Sbjct:  152 GEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETE-LSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPLCRLD 496

HSP 2 Score: 81.2629 bits (199), Expect = 1.071e-15
Identity = 35/83 (42.17%), Postives = 60/83 (72.29%), Query Frame = 3 
Query:  102 NIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
             ++HQ VVG +K+L++  +V+ +E++S    ELT EGE++   GS+E +++ SIP  G+ + +L++  + K+GF+KAMSNKWI
Sbjct:   31 GMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWI 113
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Human
Match: FARSA (phenylalanyl-tRNA synthetase subunit alpha [Source:HGNC Symbol;Acc:HGNC:3592])

HSP 1 Score: 466.463 bits (1199), Expect = 1.581e-159
Identity = 212/346 (61.27%), Postives = 278/346 (80.35%), Query Frame = 2 
Query:  449 GDQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYN-LHGGIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            G++  ++ +KRKL+ +VT+K + + KG  FST+  +     ++ E I    +    FKPYN L  G++    +LHPLLK+R ++++I +E+GF EMPT+N++ESSFWNFDALFQPQ H ARD HDTF+L +P ++ Q P +Y++RV   HS+GGYGSQGY+  WKL+EARKNLLRTHTT+ SAR LYRL+Q+K FTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++AD GL+L +L   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP +V+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +P+CRLD
Sbjct:  192 GEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETE-LSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPLCRLD 536

HSP 2 Score: 82.8037 bits (203), Expect = 4.122e-16
Identity = 41/118 (34.75%), Postives = 73/118 (61.86%), Query Frame = 3 
Query:    3 FTMS--TPIVPSVLELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
            F M+  TP+  + L L +    +    +   +    ++HQ VVG +K+L++  +V+ +E++S    ELT EGE++   GS+E +++ SIP  G+ + +L++  + K+GF+KAMSNKWI
Sbjct:   36 FKMAVHTPVPVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWI 153
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Human
Match: FARSA (phenylalanyl-tRNA synthetase subunit alpha [Source:HGNC Symbol;Acc:HGNC:3592])

HSP 1 Score: 443.736 bits (1140), Expect = 1.116e-151
Identity = 197/290 (67.93%), Postives = 248/290 (85.52%), Query Frame = 2 
Query:  617 FKPYN-LHGGIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            FKPYN L  G++    +LHPLLK+R ++++I +E+GF EMPT+N++ESSFWNFDALFQPQ H ARD HDTF+L +P ++ Q P +Y++RV   HS+GGYGSQGY+  WKL+EARKNLLRTHTT+ SAR LYRL+Q+K FTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++AD GL+L +L   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP +V+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +P+CRLD
Sbjct:  176 FKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPLCRLD 465

HSP 2 Score: 81.2629 bits (199), Expect = 9.897e-16
Identity = 35/83 (42.17%), Postives = 60/83 (72.29%), Query Frame = 3 
Query:  102 NIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
             ++HQ VVG +K+L++  +V+ +E++S    ELT EGE++   GS+E +++ SIP  G+ + +L++  + K+GF+KAMSNKWI
Sbjct:   31 GMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWI 113
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Human
Match: FARSA (phenylalanyl-tRNA synthetase subunit alpha [Source:HGNC Symbol;Acc:HGNC:3592])

HSP 1 Score: 416.772 bits (1070), Expect = 1.732e-142
Identity = 190/312 (60.90%), Postives = 249/312 (79.81%), Query Frame = 2 
Query:  449 GDQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYN-LHGGIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLE 1381
            G++  ++ +KRKL+ +VT+K + + KG  FST+  +     ++ E I    +    FKPYN L  G++    +LHPLLK+R ++++I +E+GF EMPT+N++ESSFWNFDALFQPQ H ARD HDTF+L +P ++ Q P +Y++RV   HS+GGYGSQGY+  WKL+EARKNLLRTHTT+ SAR LYRL+Q+K FTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++AD GL+L +L   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP +V+VIAWGLSLE
Sbjct:   68 GEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETE-LSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLE 378
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Human
Match: FARSA (phenylalanyl-tRNA synthetase subunit alpha [Source:HGNC Symbol;Acc:HGNC:3592])

HSP 1 Score: 218.394 bits (555), Expect = 2.280e-68
Identity = 97/137 (70.80%), Postives = 119/137 (86.86%), Query Frame = 2 
Query:  740 NNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLA 1150
            +N++ESSFWNFDALFQPQ H ARD HDTF+L +P ++ Q P +Y++RV   HS+GGYGSQGY+  WKL+EARKNLLRTHTT+ SAR LYRL+Q+K FTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++AD GL+L 
Sbjct:    2 DNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLG 138
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Celegans
Match: fars-1 (Phenylalanyl Amino-acyl tRNA Synthetase [Source:UniProtKB/TrEMBL;Acc:Q86B36])

HSP 1 Score: 452.981 bits (1164), Expect = 1.921e-155
Identity = 214/333 (64.26%), Postives = 260/333 (78.08%), Query Frame = 2 
Query:  488 IKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            I +V +K  ++ KG  F+T+  +   A +T E I    +   +FK YN    G+V    +LHPL+K+R E+++I   +GF EM TN YVESSFWNFDALFQPQ H ARDAHDTF++S+P  S +FP++YLERV  VHSKGGYGS GY   WK+EEA+KN+LRTHTTAVSAR LY+L+QE  F P K FSI+RVFRNE+LDATHLAEFHQVEG+IA++ LSLA+L     EFFKKLG+  +RFKP YNPYTEPSMEIFA+H GL KW+E+GNSGMFRPEMLLPMGLP+DVNV  +GLSLERPTMIKY IN+IR+L G K+DLN V+ NPICRLD
Sbjct:  165 ISEVNIKALVVSKGTSFTTSLAKQE-ADLTPEMIASGSWKDMQFKKYNFDSLGVVPSSGHLHPLMKVRSEFRQIFFSMGFSEMATNRYVESSFWNFDALFQPQQHPARDAHDTFFVSDPAISTKFPEDYLERVKTVHSKGGYGSAGYNYDWKIEEAQKNVLRTHTTAVSARQLYQLAQEG-FRPSKLFSIDRVFRNETLDATHLAEFHQVEGVIAEKNLSLAHLIGIFTEFFKKLGITNLRFKPTYNPYTEPSMEIFAYHQGLTKWVEIGNSGMFRPEMLLPMGLPADVNVAGYGLSLERPTMIKYGINNIRDLFGSKIDLNVVYNNPICRLD 495

HSP 2 Score: 67.3958 bits (163), Expect = 9.845e-12
Identity = 35/104 (33.65%), Postives = 62/104 (59.62%), Query Frame = 3 
Query:   39 ELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVVSEMK-SYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
            ++L ++ E+   +++  ++  N+DHQ V+G +K+L + E V++    +  +LELT EG Q  N GS E+ ++  +  +G  + D+ K    KIG AKAM  KW+
Sbjct:   16 QILDFLQESNEFNSIQLAQQWNLDHQKVIGAIKSLLANEGVLTTKDVTEKRLELTNEGVQFANEGSPEYLVFEFVGTDGAAQADIQKKPFGKIGMAKAMQFKWV 119
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Celegans
Match: fars-1 (Phenylalanyl Amino-acyl tRNA Synthetase [Source:UniProtKB/TrEMBL;Acc:Q86B36])

HSP 1 Score: 452.981 bits (1164), Expect = 1.921e-155
Identity = 214/333 (64.26%), Postives = 260/333 (78.08%), Query Frame = 2 
Query:  488 IKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            I +V +K  ++ KG  F+T+  +   A +T E I    +   +FK YN    G+V    +LHPL+K+R E+++I   +GF EM TN YVESSFWNFDALFQPQ H ARDAHDTF++S+P  S +FP++YLERV  VHSKGGYGS GY   WK+EEA+KN+LRTHTTAVSAR LY+L+QE  F P K FSI+RVFRNE+LDATHLAEFHQVEG+IA++ LSLA+L     EFFKKLG+  +RFKP YNPYTEPSMEIFA+H GL KW+E+GNSGMFRPEMLLPMGLP+DVNV  +GLSLERPTMIKY IN+IR+L G K+DLN V+ NPICRLD
Sbjct:  165 ISEVNIKALVVSKGTSFTTSLAKQE-ADLTPEMIASGSWKDMQFKKYNFDSLGVVPSSGHLHPLMKVRSEFRQIFFSMGFSEMATNRYVESSFWNFDALFQPQQHPARDAHDTFFVSDPAISTKFPEDYLERVKTVHSKGGYGSAGYNYDWKIEEAQKNVLRTHTTAVSARQLYQLAQEG-FRPSKLFSIDRVFRNETLDATHLAEFHQVEGVIAEKNLSLAHLIGIFTEFFKKLGITNLRFKPTYNPYTEPSMEIFAYHQGLTKWVEIGNSGMFRPEMLLPMGLPADVNVAGYGLSLERPTMIKYGINNIRDLFGSKIDLNVVYNNPICRLD 495

HSP 2 Score: 67.3958 bits (163), Expect = 9.845e-12
Identity = 35/104 (33.65%), Postives = 62/104 (59.62%), Query Frame = 3 
Query:   39 ELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVVSEMK-SYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
            ++L ++ E+   +++  ++  N+DHQ V+G +K+L + E V++    +  +LELT EG Q  N GS E+ ++  +  +G  + D+ K    KIG AKAM  KW+
Sbjct:   16 QILDFLQESNEFNSIQLAQQWNLDHQKVIGAIKSLLANEGVLTTKDVTEKRLELTNEGVQFANEGSPEYLVFEFVGTDGAAQADIQKKPFGKIGMAKAMQFKWV 119
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Celegans
Match: fars-1 (Phenylalanyl Amino-acyl tRNA Synthetase [Source:UniProtKB/TrEMBL;Acc:Q86B36])

HSP 1 Score: 452.981 bits (1164), Expect = 1.287e-154
Identity = 214/333 (64.26%), Postives = 260/333 (78.08%), Query Frame = 2 
Query:  488 IKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            I +V +K  ++ KG  F+T+  +   A +T E I    +   +FK YN    G+V    +LHPL+K+R E+++I   +GF EM TN YVESSFWNFDALFQPQ H ARDAHDTF++S+P  S +FP++YLERV  VHSKGGYGS GY   WK+EEA+KN+LRTHTTAVSAR LY+L+QE  F P K FSI+RVFRNE+LDATHLAEFHQVEG+IA++ LSLA+L     EFFKKLG+  +RFKP YNPYTEPSMEIFA+H GL KW+E+GNSGMFRPEMLLPMGLP+DVNV  +GLSLERPTMIKY IN+IR+L G K+DLN V+ NPICRLD
Sbjct:  221 ISEVNIKALVVSKGTSFTTSLAKQE-ADLTPEMIASGSWKDMQFKKYNFDSLGVVPSSGHLHPLMKVRSEFRQIFFSMGFSEMATNRYVESSFWNFDALFQPQQHPARDAHDTFFVSDPAISTKFPEDYLERVKTVHSKGGYGSAGYNYDWKIEEAQKNVLRTHTTAVSARQLYQLAQEG-FRPSKLFSIDRVFRNETLDATHLAEFHQVEGVIAEKNLSLAHLIGIFTEFFKKLGITNLRFKPTYNPYTEPSMEIFAYHQGLTKWVEIGNSGMFRPEMLLPMGLPADVNVAGYGLSLERPTMIKYGINNIRDLFGSKIDLNVVYNNPICRLD 551

HSP 2 Score: 67.781 bits (164), Expect = 9.835e-12
Identity = 35/104 (33.65%), Postives = 62/104 (59.62%), Query Frame = 3 
Query:   39 ELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVVSEMK-SYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
            ++L ++ E+   +++  ++  N+DHQ V+G +K+L + E V++    +  +LELT EG Q  N GS E+ ++  +  +G  + D+ K    KIG AKAM  KW+
Sbjct:   72 QILDFLQESNEFNSIQLAQQWNLDHQKVIGAIKSLLANEGVLTTKDVTEKRLELTNEGVQFANEGSPEYLVFEFVGTDGAAQADIQKKPFGKIGMAKAMQFKWV 175
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Celegans
Match: fars-1 (Phenylalanyl Amino-acyl tRNA Synthetase [Source:UniProtKB/TrEMBL;Acc:Q86B36])

HSP 1 Score: 452.981 bits (1164), Expect = 1.287e-154
Identity = 214/333 (64.26%), Postives = 260/333 (78.08%), Query Frame = 2 
Query:  488 IKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            I +V +K  ++ KG  F+T+  +   A +T E I    +   +FK YN    G+V    +LHPL+K+R E+++I   +GF EM TN YVESSFWNFDALFQPQ H ARDAHDTF++S+P  S +FP++YLERV  VHSKGGYGS GY   WK+EEA+KN+LRTHTTAVSAR LY+L+QE  F P K FSI+RVFRNE+LDATHLAEFHQVEG+IA++ LSLA+L     EFFKKLG+  +RFKP YNPYTEPSMEIFA+H GL KW+E+GNSGMFRPEMLLPMGLP+DVNV  +GLSLERPTMIKY IN+IR+L G K+DLN V+ NPICRLD
Sbjct:  221 ISEVNIKALVVSKGTSFTTSLAKQE-ADLTPEMIASGSWKDMQFKKYNFDSLGVVPSSGHLHPLMKVRSEFRQIFFSMGFSEMATNRYVESSFWNFDALFQPQQHPARDAHDTFFVSDPAISTKFPEDYLERVKTVHSKGGYGSAGYNYDWKIEEAQKNVLRTHTTAVSARQLYQLAQEG-FRPSKLFSIDRVFRNETLDATHLAEFHQVEGVIAEKNLSLAHLIGIFTEFFKKLGITNLRFKPTYNPYTEPSMEIFAYHQGLTKWVEIGNSGMFRPEMLLPMGLPADVNVAGYGLSLERPTMIKYGINNIRDLFGSKIDLNVVYNNPICRLD 551

HSP 2 Score: 67.781 bits (164), Expect = 9.835e-12
Identity = 35/104 (33.65%), Postives = 62/104 (59.62%), Query Frame = 3 
Query:   39 ELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVVSEMK-SYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
            ++L ++ E+   +++  ++  N+DHQ V+G +K+L + E V++    +  +LELT EG Q  N GS E+ ++  +  +G  + D+ K    KIG AKAM  KW+
Sbjct:   72 QILDFLQESNEFNSIQLAQQWNLDHQKVIGAIKSLLANEGVLTTKDVTEKRLELTNEGVQFANEGSPEYLVFEFVGTDGAAQADIQKKPFGKIGMAKAMQFKWV 175
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Celegans
Match: fars-2 (Phenylalanyl Amino-acyl tRNA Synthetase [Source:UniProtKB/TrEMBL;Acc:G5EEN1])

HSP 1 Score: 68.1662 bits (165), Expect = 5.399e-12
Identity = 67/267 (25.09%), Postives = 107/267 (40.07%), Query Frame = 2 
Query:  749 VESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEG--------LIADR--GLSLANLQSTIA---------------------------EFFKKL-----GMD-RIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIR 1420
            V +++ NFD+L  P+DH +R   DT+Y++                                        ++ LR HT+A    ++       +     +  I  V+R + +D TH   FHQ+EG        L+  +  G ++A L ST A                           +F + L     G D   R+  AY P+T PS E+  F+N  G+W+EV   G+   ++L   G+   +   A+G+ LER  M+ Y I DIR
Sbjct:   88 VVTTYQNFDSLLTPEDHVSRRPSDTYYVN---------------------------------------HEHCLRAHTSAHQHNLM-------QSGLDAFLVIGDVYRRDEVDRTHYPCFHQIEGVRLYSKDELLGKKPDGKNVAELFSTAASAATERSPEKQEKHTLDATKAAEIQLKQFLENLCDELFGKDAEKRWVDAYFPFTHPSWELEVFYN--GQWLEVLGCGIMEQKLLESAGVTDKIG-WAFGIGLERIAMVLYGIPDIR 305
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Fly
Match: alpha-PheRS (gene:FBgn0030007 transcript:FBtr0071190)

HSP 1 Score: 451.825 bits (1161), Expect = 6.216e-155
Identity = 228/351 (64.96%), Postives = 277/351 (78.92%), Query Frame = 2 
Query:  449 GDQL----LNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLS-QEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            GDQL    + D+KKRKL+++ T K F++ +GP+F+TT  +    +  E     L ++  KFK YN    G      +LHPLLK+R E+++I +E+GF EMPTNNYVESSFWNFDAL+QPQ H ARDAHDTF+++ P KS +FPQ+YLERV  VHS GGYGS+GY   WKLEEA+KNLLRTHTTAVSARMLY+L+ QE  F   KYFSI++VFRNE+LDATHLAEFHQVEG+IAD GL+L +L  T+ EFF+KLG+ ++ FKPAYNPYTEPSMEIF +H GL KWIEVGNSG+FRPEMLLPMGLP +VNVIAWGLSLERPTMIKY IN+IR+L+G K+DL  V   PICRLD
Sbjct:  147 GDQLPAKEVADFKKRKLLQETTTKSFVLARGPEFATTLTKLETDLTVEMLANGL-WDQLKFKAYNFDALGAPPTRGHLHPLLKVRTEFRQIFLEMGFSEMPTNNYVESSFWNFDALYQPQQHPARDAHDTFFVNHPAKSHKFPQDYLERVKKVHSVGGYGSKGYGYDWKLEEAQKNLLRTHTTAVSARMLYKLANQEGGFKAAKYFSIDKVFRNETLDATHLAEFHQVEGVIADVGLTLGDLIGTLYEFFRKLGITQLEFKPAYNPYTEPSMEIFCYHPGLAKWIEVGNSGVFRPEMLLPMGLPENVNVIAWGLSLERPTMIKYGINNIRDLVGPKVDLKMVEEGPICRLD 496

HSP 2 Score: 67.781 bits (164), Expect = 1.062e-11
Identity = 38/106 (35.85%), Postives = 61/106 (57.55%), Query Frame = 3 
Query:   39 ELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLES-TEKVVSEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKT--ANDKIGFAKAMSNKWI 347
             +L+++   +   TL  +     DHQ +VG LK++++  E V +E  ++  L LT+EG  V   GS+E  +Y  +P  GI +  L+    AN K+GF+KAMS+ WI
Sbjct:    8 RILQHLETTDKADTLDLAALFAEDHQKIVGSLKSIQAHGELVTAETVTHKSLGLTDEGRAVVENGSHEALVYDLVPPEGIAQAALMAAGGANAKVGFSKAMSHGWI 113
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Zebrafish
Match: farsa (phenylalanyl-tRNA synthetase subunit alpha [Source:NCBI gene;Acc:692329])

HSP 1 Score: 474.167 bits (1219), Expect = 1.464e-163
Identity = 215/334 (64.37%), Postives = 274/334 (82.04%), Query Frame = 2 
Query:  485 LIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            L+ +VTVK + + KG  FSTT  +     +T E I   ++   KFKPYN    G+     +LHPL+K+R ++++I +E+GF EMPTNN++ESSFWNFD+LFQPQ H ARD HDTF++S+P  + +FP++YLERV  VHS+GGYGSQGY+  WK+EEA+KNLLRTHTTAVSARMLY+L+Q++KFTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++AD GL+L NL   + +FF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP  V+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +PICRLD
Sbjct:  164 LLAEVTVKSYWITKGNSFSTTITKQETE-LTPEMIASGNWKEKKFKPYNFEAMGVAPDCGHLHPLMKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDSLFQPQQHPARDQHDTFFISDPALAHEFPRDYLERVKKVHSEGGYGSQGYKYDWKIEEAQKNLLRTHTTAVSARMLYKLAQQEKFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADYGLTLGNLMGVLHQFFTKLGITKLRFKPAYNPYTEPSMEVFSYHEGLKKWVEVGNSGVFRPEMLLPMGLPEGVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPICRLD 496

HSP 2 Score: 83.5741 bits (205), Expect = 1.160e-16
Identity = 39/90 (43.33%), Postives = 63/90 (70.00%), Query Frame = 3 
Query:  102 NIDHQVVVGILKNLESTEKVVS-EMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWIGFPRKTE 368
             +DHQ+VVG +K+L++  +V+S E KS    ELT EG ++   GS+E +++++IP  G+ ++ L+K A+ K+GF+KAMSNKWI   +  E
Sbjct:   31 GVDHQLVVGAVKSLQALGEVISAEQKSSKHWELTGEGREIAEQGSHEARVFNAIPAEGLPQNQLMKMASGKVGFSKAMSNKWIRLDKAHE 120
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Xenopus
Match: farsa (phenylalanyl-tRNA synthetase subunit alpha [Source:NCBI gene;Acc:780288])

HSP 1 Score: 474.937 bits (1221), Expect = 1.438e-163
Identity = 214/341 (62.76%), Postives = 276/341 (80.94%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            N+ KKRKL+ +VTVK + + KG  FST+  +     +T E I    +   +FK YN +  G++    +LHPLLK+R ++++I +E+GF EMPTNN++ESSFWNFDALFQPQ H ARD HDTF+L +P  + +FP EYLE+V  VHS+GGYGSQGY+  W + EA+KN+LRTHTTAVSARMLY+L+Q+K+F+PVKYFSI+RVFRNE+LDATHLAEFHQ+EG++ADRGL+L NL   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPE+LLPMGLP DVNV+ WGLSLERPTMI+Y I +IREL+G K++L  V+ +PICRLD
Sbjct:  166 NELKKRKLVNEVTVKSYWVTKGSGFSTSITKQETD-LTPEMIASGSWREKQFKGYNFNALGVMPECGHLHPLLKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDALFQPQQHPARDQHDTFFLQDPALATEFPMEYLEKVKKVHSEGGYGSQGYKYDWSIHEAQKNILRTHTTAVSARMLYKLAQQKEFSPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADRGLTLGNLMGVLKEFFHKLGITKLRFKPAYNPYTEPSMEVFSYHEGLQKWVEVGNSGLFRPELLLPMGLPEDVNVLGWGLSLERPTMIRYGIKNIRELVGHKVNLQMVYDSPICRLD 505

HSP 2 Score: 75.0998 bits (183), Expect = 8.378e-14
Identity = 37/113 (32.74%), Postives = 71/113 (62.83%), Query Frame = 3 
Query:   39 ELLKYINE--NENTSTLTFSKNNNIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWIGFPRKTE 368
            +LL+ + +  ++   +L  +    ++HQ VVG +K+L+   +++ +E +S  K EL+ EGE++   GS+E +++ S+P  G+ + +L+K    K+GF+KAMSNKW+   +  E
Sbjct:   17 QLLQRLQDGGSKGVDSLELAALLGVEHQQVVGAVKSLQCLGQIIEAEQRSSKKWELSSEGEEIAREGSHEARVFHSLPKEGLLQSELMKQPIAKVGFSKAMSNKWVRVDKAAE 129
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Xenopus
Match: farsa (phenylalanyl-tRNA synthetase subunit alpha [Source:NCBI gene;Acc:780288])

HSP 1 Score: 474.937 bits (1221), Expect = 1.653e-163
Identity = 214/341 (62.76%), Postives = 276/341 (80.94%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            N+ KKRKL+ +VTVK + + KG  FST+  +     +T E I    +   +FK YN +  G++    +LHPLLK+R ++++I +E+GF EMPTNN++ESSFWNFDALFQPQ H ARD HDTF+L +P  + +FP EYLE+V  VHS+GGYGSQGY+  W + EA+KN+LRTHTTAVSARMLY+L+Q+K+F+PVKYFSI+RVFRNE+LDATHLAEFHQ+EG++ADRGL+L NL   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPE+LLPMGLP DVNV+ WGLSLERPTMI+Y I +IREL+G K++L  V+ +PICRLD
Sbjct:  159 NELKKRKLVNEVTVKSYWVTKGSGFSTSITKQETD-LTPEMIASGSWREKQFKGYNFNALGVMPECGHLHPLLKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDALFQPQQHPARDQHDTFFLQDPALATEFPMEYLEKVKKVHSEGGYGSQGYKYDWSIHEAQKNILRTHTTAVSARMLYKLAQQKEFSPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADRGLTLGNLMGVLKEFFHKLGITKLRFKPAYNPYTEPSMEVFSYHEGLQKWVEVGNSGLFRPELLLPMGLPEDVNVLGWGLSLERPTMIRYGIKNIRELVGHKVNLQMVYDSPICRLD 498

HSP 2 Score: 75.0998 bits (183), Expect = 9.600e-14
Identity = 36/106 (33.96%), Postives = 69/106 (65.09%), Query Frame = 3 
Query:   39 ELLKYINE--NENTSTLTFSKNNNIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
            +LL+ + +  ++   +L  +    ++HQ VVG +K+L+   +++ +E +S  K EL+ EGE++   GS+E +++ S+P  G+ + +L+K    K+GF+KAMSNKW+
Sbjct:    9 QLLQRLQDGGSKGVDSLELAALLGVEHQQVVGAVKSLQCLGQIIEAEQRSSKKWELSSEGEEIAREGSHEARVFHSLPKEGLLQSELMKQPIAKVGFSKAMSNKWV 114
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Xenopus
Match: farsa (phenylalanyl-tRNA synthetase subunit alpha [Source:NCBI gene;Acc:780288])

HSP 1 Score: 474.937 bits (1221), Expect = 2.042e-163
Identity = 214/341 (62.76%), Postives = 276/341 (80.94%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            N+ KKRKL+ +VTVK + + KG  FST+  +     +T E I    +   +FK YN +  G++    +LHPLLK+R ++++I +E+GF EMPTNN++ESSFWNFDALFQPQ H ARD HDTF+L +P  + +FP EYLE+V  VHS+GGYGSQGY+  W + EA+KN+LRTHTTAVSARMLY+L+Q+K+F+PVKYFSI+RVFRNE+LDATHLAEFHQ+EG++ADRGL+L NL   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPE+LLPMGLP DVNV+ WGLSLERPTMI+Y I +IREL+G K++L  V+ +PICRLD
Sbjct:  169 NELKKRKLVNEVTVKSYWVTKGSGFSTSITKQETD-LTPEMIASGSWREKQFKGYNFNALGVMPECGHLHPLLKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDALFQPQQHPARDQHDTFFLQDPALATEFPMEYLEKVKKVHSEGGYGSQGYKYDWSIHEAQKNILRTHTTAVSARMLYKLAQQKEFSPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADRGLTLGNLMGVLKEFFHKLGITKLRFKPAYNPYTEPSMEVFSYHEGLQKWVEVGNSGLFRPELLLPMGLPEDVNVLGWGLSLERPTMIRYGIKNIRELVGHKVNLQMVYDSPICRLD 508

HSP 2 Score: 75.485 bits (184), Expect = 6.952e-14
Identity = 37/113 (32.74%), Postives = 71/113 (62.83%), Query Frame = 3 
Query:   39 ELLKYINE--NENTSTLTFSKNNNIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWIGFPRKTE 368
            +LL+ + +  ++   +L  +    ++HQ VVG +K+L+   +++ +E +S  K EL+ EGE++   GS+E +++ S+P  G+ + +L+K    K+GF+KAMSNKW+   +  E
Sbjct:    9 QLLQRLQDGGSKGVDSLELAALLGVEHQQVVGAVKSLQCLGQIIEAEQRSSKKWELSSEGEEIAREGSHEARVFHSLPKEGLLQSELMKQPIAKVGFSKAMSNKWVRVDKAAE 121
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Xenopus
Match: farsa (phenylalanyl-tRNA synthetase subunit alpha [Source:NCBI gene;Acc:780288])

HSP 1 Score: 474.552 bits (1220), Expect = 2.291e-163
Identity = 214/341 (62.76%), Postives = 276/341 (80.94%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            N+ KKRKL+ +VTVK + + KG  FST+  +     +T E I    +   +FK YN +  G++    +LHPLLK+R ++++I +E+GF EMPTNN++ESSFWNFDALFQPQ H ARD HDTF+L +P  + +FP EYLE+V  VHS+GGYGSQGY+  W + EA+KN+LRTHTTAVSARMLY+L+Q+K+F+PVKYFSI+RVFRNE+LDATHLAEFHQ+EG++ADRGL+L NL   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPE+LLPMGLP DVNV+ WGLSLERPTMI+Y I +IREL+G K++L  V+ +PICRLD
Sbjct:  159 NELKKRKLVNEVTVKSYWVTKGSGFSTSITKQETD-LTPEMIASGSWREKQFKGYNFNALGVMPECGHLHPLLKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDALFQPQQHPARDQHDTFFLQDPALATEFPMEYLEKVKKVHSEGGYGSQGYKYDWSIHEAQKNILRTHTTAVSARMLYKLAQQKEFSPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADRGLTLGNLMGVLKEFFHKLGITKLRFKPAYNPYTEPSMEVFSYHEGLQKWVEVGNSGLFRPELLLPMGLPEDVNVLGWGLSLERPTMIRYGIKNIRELVGHKVNLQMVYDSPICRLD 498

HSP 2 Score: 75.0998 bits (183), Expect = 8.558e-14
Identity = 36/106 (33.96%), Postives = 69/106 (65.09%), Query Frame = 3 
Query:   39 ELLKYINE--NENTSTLTFSKNNNIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
            +LL+ + +  ++   +L  +    ++HQ VVG +K+L+   +++ +E +S  K EL+ EGE++   GS+E +++ S+P  G+ + +L+K    K+GF+KAMSNKW+
Sbjct:    9 QLLQRLQDGGSKGVDSLELAALLGVEHQQVVGAVKSLQCLGQIIEAEQRSSKKWELSSEGEEIAREGSHEARVFHSLPKEGLLQSELMKQPIAKVGFSKAMSNKWV 114
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Xenopus
Match: farsa (phenylalanyl-tRNA synthetase subunit alpha [Source:NCBI gene;Acc:780288])

HSP 1 Score: 473.011 bits (1216), Expect = 3.575e-163
Identity = 214/341 (62.76%), Postives = 276/341 (80.94%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            N+ KKRKL+ +VTVK + + KG  FST+  +     +T E I    +   +FK YN +  G++    +LHPLLK+R ++++I +E+GF EMPTNN++ESSFWNFDALFQPQ H ARD HDTF+L +P  + +FP EYLE+V  VHS+GGYGSQGY+  W + EA+KN+LRTHTTAVSARMLY+L+Q+K+F+PVKYFSI+RVFRNE+LDATHLAEFHQ+EG++ADRGL+L NL   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPE+LLPMGLP DVNV+ WGLSLERPTMI+Y I +IREL+G K++L  V+ +PICRLD
Sbjct:  134 NELKKRKLVNEVTVKSYWVTKGSGFSTSITKQETD-LTPEMIASGSWREKQFKGYNFNALGVMPECGHLHPLLKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDALFQPQQHPARDQHDTFFLQDPALATEFPMEYLEKVKKVHSEGGYGSQGYKYDWSIHEAQKNILRTHTTAVSARMLYKLAQQKEFSPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADRGLTLGNLMGVLKEFFHKLGITKLRFKPAYNPYTEPSMEVFSYHEGLQKWVEVGNSGLFRPELLLPMGLPEDVNVLGWGLSLERPTMIRYGIKNIRELVGHKVNLQMVYDSPICRLD 473

HSP 2 Score: 62.003 bits (149), Expect = 1.387e-9
Identity = 26/61 (42.62%), Postives = 43/61 (70.49%), Query Frame = 3 
Query:  165 SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
            +E +S  K EL+ EGE++   GS+E +++ S+P  G+ + +L+K    K+GF+KAMSNKW+
Sbjct:   29 AEQRSSKKWELSSEGEEIAREGSHEARVFHSLPKEGLLQSELMKQPIAKVGFSKAMSNKWV 89
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Mouse
Match: Farsa (phenylalanyl-tRNA synthetase, alpha subunit [Source:MGI Symbol;Acc:MGI:1913840])

HSP 1 Score: 466.077 bits (1198), Expect = 3.563e-160
Identity = 212/341 (62.17%), Postives = 272/341 (79.77%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            N+ +KRKL+ +V +K + + KG  FST+  +   A ++ E I    +    FKPYN    G++    +LHPLLK+R ++++I +E+GF EMPT+N++ESSFWNFDALFQPQ H ARD HDTF+L +P ++ Q P  Y++RV   HS+GGYGSQGY+  WKLEEARKNLLRTHTTA SAR LY+L+Q+K FTP KYFSI+RVFRNE+LDATHLAEFHQ+EG+IAD GL+L +L   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP +V+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +P+CRLD
Sbjct:  157 NELRKRKLLTEVILKTYWVSKGKAFSTSVSKQE-AELSPEMISSGSWRDRPFKPYNFSARGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMGYVQRVKRTHSQGGYGSQGYKYTWKLEEARKNLLRTHTTAASARALYQLAQKKPFTPAKYFSIDRVFRNETLDATHLAEFHQIEGVIADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPVCRLD 496

HSP 2 Score: 80.1073 bits (196), Expect = 1.693e-15
Identity = 35/83 (42.17%), Postives = 60/83 (72.29%), Query Frame = 3 
Query:  102 NIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
             ++HQ VVG +K+L++  +V+ +E++S    ELT EGE++   GS+E +++ SIP+ G+ + +L+   + K+GF+KAMSNKWI
Sbjct:   31 GVEHQAVVGAVKSLQALGEVIEAELRSTKCWELTTEGEEIAREGSHEARVFRSIPLEGLVQSELMHLPSGKVGFSKAMSNKWI 113
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Mouse
Match: Farsa (phenylalanyl-tRNA synthetase, alpha subunit [Source:MGI Symbol;Acc:MGI:1913840])

HSP 1 Score: 461.455 bits (1186), Expect = 2.816e-158
Identity = 212/341 (62.17%), Postives = 271/341 (79.47%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            N+ +KRKL+ +V +K + + KG  FST+  +   A ++ E I    +    FKPYN    G++    +LHPLLK+R ++++I +E+GF EMPT+N++ESSFWNFDALFQPQ H ARD HDTF+L +P ++ Q P  Y++RV   HS+GGYGSQGY+  WKLEEARKNLLRTHTTA SAR LY+L+Q K FTP KYFSI+RVFRNE+LDATHLAEFHQ+EG+IAD GL+L +L   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP +V+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +P+CRLD
Sbjct:  157 NELRKRKLLTEVILKTYWVSKGKAFSTSVSKQE-AELSPEMISSGSWRDRPFKPYNFSARGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMGYVQRVKRTHSQGGYGSQGYKYTWKLEEARKNLLRTHTTAASARALYQLAQ-KPFTPAKYFSIDRVFRNETLDATHLAEFHQIEGVIADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPVCRLD 495

HSP 2 Score: 80.1073 bits (196), Expect = 1.615e-15
Identity = 35/83 (42.17%), Postives = 60/83 (72.29%), Query Frame = 3 
Query:  102 NIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
             ++HQ VVG +K+L++  +V+ +E++S    ELT EGE++   GS+E +++ SIP+ G+ + +L+   + K+GF+KAMSNKWI
Sbjct:   31 GVEHQAVVGAVKSLQALGEVIEAELRSTKCWELTTEGEEIAREGSHEARVFRSIPLEGLVQSELMHLPSGKVGFSKAMSNKWI 113
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Mouse
Match: Fars2 (phenylalanine-tRNA synthetase 2 (mitochondrial) [Source:MGI Symbol;Acc:MGI:1917205])

HSP 1 Score: 61.6178 bits (148), Expect = 1.143e-9
Identity = 62/261 (23.75%), Postives = 99/261 (37.93%), Query Frame = 2 
Query:  749 VESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGL-------------------IADRGLSLANLQST-------IAEFFKKLGMDR-----------IRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIR 1420
            V +++ NFD+L  P DH +R   D +YL+                                       R ++LR HT+A    +L+            +  +  V+R + +D  H   FHQ+EG+                   + + G   A+ Q T       + EF  K  + R           +R+   Y P+T PS E+    N  G+W+EV   G+   +++   G    +   A+GL LER  M+ Y I DIR
Sbjct:  119 VVTTWQNFDSLLIPADHPSRKKGDNYYLN---------------------------------------RAHMLRAHTSAHQWDLLHAGLN-------AFLVVGDVYRRDQIDCQHYPVFHQLEGVRLFSKHELFAGVKDGESLQLFEEGSRSAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEI--NFRGEWLEVLGCGVMEQQLVNSAGAQDRIG-WAFGLGLERLAMVLYDIPDIR 330
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Mouse
Match: Fars2 (phenylalanine-tRNA synthetase 2 (mitochondrial) [Source:MGI Symbol;Acc:MGI:1917205])

HSP 1 Score: 61.6178 bits (148), Expect = 1.143e-9
Identity = 62/261 (23.75%), Postives = 99/261 (37.93%), Query Frame = 2 
Query:  749 VESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGL-------------------IADRGLSLANLQST-------IAEFFKKLGMDR-----------IRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIR 1420
            V +++ NFD+L  P DH +R   D +YL+                                       R ++LR HT+A    +L+            +  +  V+R + +D  H   FHQ+EG+                   + + G   A+ Q T       + EF  K  + R           +R+   Y P+T PS E+    N  G+W+EV   G+   +++   G    +   A+GL LER  M+ Y I DIR
Sbjct:  119 VVTTWQNFDSLLIPADHPSRKKGDNYYLN---------------------------------------RAHMLRAHTSAHQWDLLHAGLN-------AFLVVGDVYRRDQIDCQHYPVFHQLEGVRLFSKHELFAGVKDGESLQLFEEGSRSAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEI--NFRGEWLEVLGCGVMEQQLVNSAGAQDRIG-WAFGLGLERLAMVLYDIPDIR 330
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Mouse
Match: Fars2 (phenylalanine-tRNA synthetase 2 (mitochondrial) [Source:MGI Symbol;Acc:MGI:1917205])

HSP 1 Score: 61.6178 bits (148), Expect = 1.143e-9
Identity = 62/261 (23.75%), Postives = 99/261 (37.93%), Query Frame = 2 
Query:  749 VESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGL-------------------IADRGLSLANLQST-------IAEFFKKLGMDR-----------IRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIR 1420
            V +++ NFD+L  P DH +R   D +YL+                                       R ++LR HT+A    +L+            +  +  V+R + +D  H   FHQ+EG+                   + + G   A+ Q T       + EF  K  + R           +R+   Y P+T PS E+    N  G+W+EV   G+   +++   G    +   A+GL LER  M+ Y I DIR
Sbjct:  119 VVTTWQNFDSLLIPADHPSRKKGDNYYLN---------------------------------------RAHMLRAHTSAHQWDLLHAGLN-------AFLVVGDVYRRDQIDCQHYPVFHQLEGVRLFSKHELFAGVKDGESLQLFEEGSRSAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEI--NFRGEWLEVLGCGVMEQQLVNSAGAQDRIG-WAFGLGLERLAMVLYDIPDIR 330
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. UniProt/SwissProt
Match: sp|Q7SYV0|SYFAB_XENLA (Phenylalanine--tRNA ligase alpha subunit B OS=Xenopus laevis OX=8355 GN=farsa-b PE=2 SV=1)

HSP 1 Score: 476.863 bits (1226), Expect = 1.022e-163
Identity = 216/343 (62.97%), Postives = 276/343 (80.47%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD*C 1489
            N+ KKRKL+ +VTVK + + KG  FST+  +     +T E I    +   +FK YN +  G++    +LHPLLK+R ++++I +E+GF EMPTNN++ESSFWNFDALFQPQ H ARD HDTF+L +P  + +FP EYLERV  VHS+GGYGSQGY+  W + EA+KN+LRTHTTAVSARMLY+L+ +K+FTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++ADRGL+L NL   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPE+LLPMGLP DVNV+ WGLSLERPTMI+Y I +IREL+G K++L  V+ +PICRLD C
Sbjct:  158 NELKKRKLVNEVTVKSYWVTKGSGFSTSITKQETD-LTPEMIASGSWREKQFKGYNFNALGVMPECGHLHPLLKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDALFQPQQHPARDQHDTFFLQDPALATEFPMEYLERVKKVHSEGGYGSQGYKYDWSIHEAQKNILRTHTTAVSARMLYKLAHQKEFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADRGLTLGNLMGVLKEFFHKLGITKLRFKPAYNPYTEPSMEVFSYHEGLKKWVEVGNSGLFRPELLLPMGLPGDVNVLGWGLSLERPTMIRYGIKNIRELVGHKVNLQMVYDSPICRLDAC 499

HSP 2 Score: 82.4185 bits (202), Expect = 2.110e-15
Identity = 38/98 (38.78%), Postives = 66/98 (67.35%), Query Frame = 3 
Query:   57 NENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
             +N+   TL  +   ++DHQ VVG +K+L+   +++ +E +S  K EL+ EGE++  +GS+E +++ S+P  G+ + +L+K    K+GF+KAMSNKWI
Sbjct:   17 GDNKGVDTLELAAVLSVDHQQVVGAVKSLQCLGEIIEAEQRSSKKWELSSEGEEIAQVGSHEARVFQSLPKEGLLQAELMKLPFAKVGFSKAMSNKWI 114
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. UniProt/SwissProt
Match: sp|Q1JPX3|SYFA_DANRE (Phenylalanine--tRNA ligase alpha subunit OS=Danio rerio OX=7955 GN=farsa PE=2 SV=2)

HSP 1 Score: 474.167 bits (1219), Expect = 1.105e-162
Identity = 215/334 (64.37%), Postives = 274/334 (82.04%), Query Frame = 2 
Query:  485 LIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            L+ +VTVK + + KG  FSTT  +     +T E I   ++   KFKPYN    G+     +LHPL+K+R ++++I +E+GF EMPTNN++ESSFWNFD+LFQPQ H ARD HDTF++S+P  + +FP++YLERV  VHS+GGYGSQGY+  WK+EEA+KNLLRTHTTAVSARMLY+L+Q++KFTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++AD GL+L NL   + +FF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP  V+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +PICRLD
Sbjct:  164 LLAEVTVKSYWITKGNSFSTTITKQETE-LTPEMIASGNWKEKKFKPYNFEAMGVAPDCGHLHPLMKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDSLFQPQQHPARDQHDTFFISDPALAHEFPRDYLERVKKVHSEGGYGSQGYKYDWKIEEAQKNLLRTHTTAVSARMLYKLAQQEKFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADYGLTLGNLMGVLHQFFTKLGITKLRFKPAYNPYTEPSMEVFSYHEGLKKWVEVGNSGVFRPEMLLPMGLPEGVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPICRLD 496

HSP 2 Score: 83.5741 bits (205), Expect = 8.755e-16
Identity = 39/90 (43.33%), Postives = 63/90 (70.00%), Query Frame = 3 
Query:  102 NIDHQVVVGILKNLESTEKVVS-EMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWIGFPRKTE 368
             +DHQ+VVG +K+L++  +V+S E KS    ELT EG ++   GS+E +++++IP  G+ ++ L+K A+ K+GF+KAMSNKWI   +  E
Sbjct:   31 GVDHQLVVGAVKSLQALGEVISAEQKSSKHWELTGEGREIAEQGSHEARVFNAIPAEGLPQNQLMKMASGKVGFSKAMSNKWIRLDKAHE 120
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. UniProt/SwissProt
Match: sp|Q6AZG6|SYFAA_XENLA (Phenylalanine--tRNA ligase alpha subunit A OS=Xenopus laevis OX=8355 GN=farsa-a PE=2 SV=1)

HSP 1 Score: 473.011 bits (1216), Expect = 3.824e-162
Identity = 215/341 (63.05%), Postives = 273/341 (80.06%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            N+ KKRKL+ +VTVK + + KG  FST+  +     +T E I    +   +FK YN    G++    +LHPLLK+R ++++I +E+GF EMPTNN++ESSFWNFDALFQPQ H ARD HDTF+L +P  + +FP EYLERV  VHS+GGYGSQGY+  W + EA+KN+LRTHTTAVSARMLY+L+ +K FTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++ADRGL+L NL   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPE+LLPMGLP DVNV+ WGLSLERPTMI+Y I +IREL+G K++L  V+ +PICRLD
Sbjct:  158 NELKKRKLVNEVTVKSYWVTKGSGFSTSITKQETD-LTPEMIASGSWREKQFKGYNFDALGVMPECGHLHPLLKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDALFQPQQHPARDQHDTFFLQDPALATEFPMEYLERVKKVHSEGGYGSQGYKYDWSIHEAQKNILRTHTTAVSARMLYKLALQKDFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADRGLTLGNLMGVLKEFFHKLGITKLRFKPAYNPYTEPSMEVFSYHEGLKKWVEVGNSGLFRPELLLPMGLPEDVNVLGWGLSLERPTMIRYGIKNIRELVGHKVNLQMVYDSPICRLD 497

HSP 2 Score: 74.3294 bits (181), Expect = 7.996e-13
Identity = 37/113 (32.74%), Postives = 70/113 (61.95%), Query Frame = 3 
Query:   39 ELLKYINE--NENTSTLTFSKNNNIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWIGFPRKTE 368
            +LL+ + +  ++   +L  +    ++HQ VVG +K+L+   +++ +E +S  K EL+ EGE++   GS+E +++ ++P  G+ +  L+K    K+GF+KAMSNKWI   +  E
Sbjct:    9 QLLQRLQDGGSKGVDSLELAALLGVEHQQVVGAVKSLQCLGEIIQAEQRSSKKWELSSEGEEIGREGSHEARVFQTLPKEGLLQAQLMKQPFSKVGFSKAMSNKWIRLDKAAE 121
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. UniProt/SwissProt
Match: sp|Q505J8|SYFA_RAT (Phenylalanine--tRNA ligase alpha subunit OS=Rattus norvegicus OX=10116 GN=Farsa PE=1 SV=1)

HSP 1 Score: 469.159 bits (1206), Expect = 1.936e-160
Identity = 213/341 (62.46%), Postives = 273/341 (80.06%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            N+ +KRKL+ +V +K + + KG  FST+  +   A ++ E I    +    FKPYN    G++    +LHPLLK+R ++++I +E+GF EMPT+N++ESSFWNFDALFQPQ H ARD HDTF+L +P ++ Q P +Y++RV   HS+GGYGSQGY+  WKLEEARKNLLRTHTTA SAR LYRL+Q+K FTP KYFSI+RVFRNE+LDATHLAEFHQ+EG+IAD GL+L +L   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP +V+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +P+CRLD
Sbjct:  157 NELRKRKLLTEVILKTYWVSKGKGFSTSVSKQE-AELSPEMISSGSWRDRPFKPYNFSARGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYTWKLEEARKNLLRTHTTAASARALYRLAQKKPFTPAKYFSIDRVFRNETLDATHLAEFHQIEGVIADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPVCRLD 496

HSP 2 Score: 82.4185 bits (202), Expect = 2.438e-15
Identity = 39/111 (35.14%), Postives = 74/111 (66.67%), Query Frame = 3 
Query:   18 PIVPSVLELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
            P++  +L  L+  +   +++ L  +    ++HQ VVG +K+L++  +V+ +E++S    ELT EGE++   GS+E +++ SIP+ G+ + +L++  + K+GF+KAMSNKWI
Sbjct:    5 PVLEQLLRRLEVADGGLDSAEL--ATQLGVEHQAVVGAVKSLQALGEVIEAELRSTKCWELTTEGEEIAREGSHEARVFRSIPLEGLVQSELMQLPSGKVGFSKAMSNKWI 113
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. UniProt/SwissProt
Match: sp|Q9Y285|SYFA_HUMAN (Phenylalanine--tRNA ligase alpha subunit OS=Homo sapiens OX=9606 GN=FARSA PE=1 SV=3)

HSP 1 Score: 466.848 bits (1200), Expect = 1.528e-159
Identity = 212/346 (61.27%), Postives = 278/346 (80.35%), Query Frame = 2 
Query:  449 GDQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYN-LHGGIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            G++  ++ +KRKL+ +VT+K + + KG  FST+  +     ++ E I    +    FKPYN L  G++    +LHPLLK+R ++++I +E+GF EMPT+N++ESSFWNFDALFQPQ H ARD HDTF+L +P ++ Q P +Y++RV   HS+GGYGSQGY+  WKL+EARKNLLRTHTT+ SAR LYRL+Q+K FTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++AD GL+L +L   + EFF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP +V+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +P+CRLD
Sbjct:  152 GEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETE-LSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPLCRLD 496

HSP 2 Score: 81.2629 bits (199), Expect = 5.143e-15
Identity = 35/83 (42.17%), Postives = 60/83 (72.29%), Query Frame = 3 
Query:  102 NIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
             ++HQ VVG +K+L++  +V+ +E++S    ELT EGE++   GS+E +++ SIP  G+ + +L++  + K+GF+KAMSNKWI
Sbjct:   31 GMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWI 113
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. TrEMBL
Match: A0A2H1WLT8 (SFRICE_017812 OS=Spodoptera frugiperda OX=7108 GN=SFRICE_017812 PE=4 SV=1)

HSP 1 Score: 505.753 bits (1301), Expect = 1.329e-172
Identity = 236/341 (69.21%), Postives = 283/341 (82.99%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            NDYKKRKL+++VTVK F++ KGP+FSTT  +     +T E +    + + +FKPYN    G      +LHPLLK+R E++ I +E+GF EMPTN Y+ESSFWNFDALFQPQ H ARDAHDTF++S P  + QFP EYLERV  VHS+GGYGSQGYR  WK+EEA+KNLLRTHTTAVSARMLY+L+Q+K+FTP KYFSI++VFRNE+LDATHLAEFHQVEG+IADRGL LA+L S +  FFK+LG D+++FKPAYNPYTEPSMEIFA+H GLGKWIE+GNSG+FRPEMLLPMGLP DVNVIAWGLSLERPTMIKY +N+IR+L+G K+DL  V  NPICRLD
Sbjct:  151 NDYKKRKLLQEVTVKSFLLSKGPQFSTTIKKLETD-LTSEMLLTGAWKTLQFKPYNFDALGQPPECGHLHPLLKVRSEFREIFLEMGFTEMPTNQYIESSFWNFDALFQPQQHPARDAHDTFFMSSPASTTQFPMEYLERVKKVHSEGGYGSQGYRYDWKIEEAQKNLLRTHTTAVSARMLYKLAQQKEFTPQKYFSIDKVFRNETLDATHLAEFHQVEGVIADRGLGLADLISVLDTFFKRLGFDKLQFKPAYNPYTEPSMEIFAYHTGLGKWIEIGNSGVFRPEMLLPMGLPEDVNVIAWGLSLERPTMIKYGLNNIRDLVGPKVDLQMVENNPICRLD 490

HSP 2 Score: 85.5001 bits (210), Expect = 7.021e-14
Identity = 43/104 (41.35%), Postives = 67/104 (64.42%), Query Frame = 3 
Query:   39 ELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVVSEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKT-ANDKIGFAKAMSNKWI 347
             +L Y+++ +   T+  +     DHQ +VG +K+LE+ E VVSE     + ELT EG+QV +LGS+E  +Y+ IP  G+ + +++KT  N K+GF+KAMS  WI
Sbjct:    6 RILHYLDKTDKVDTIKLANEFEEDHQKIVGAVKSLEALEMVVSEPTKSTRWELTGEGQQVADLGSHEAVLYNHIPETGMPQAEVMKTVPNAKVGFSKAMSAGWI 109
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. TrEMBL
Match: A0A2A4JJB0 (AA_TRNA_LIGASE_II domain-containing protein OS=Heliothis virescens OX=7102 GN=B5V51_1799 PE=4 SV=1)

HSP 1 Score: 502.671 bits (1293), Expect = 2.138e-171
Identity = 234/345 (67.83%), Postives = 285/345 (82.61%), Query Frame = 2 
Query:  452 DQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            D + NDYKKRKL+++VT+K F++ KGP+F+TT  +     +T E +    + + +FKPYN    G      +LHPLLK+R E++ I +E+GF EMPTN Y+ESSFWNFDALFQPQ H ARDAHDTF++S P  + +FP EYLERV  VHS+GGYGSQGYR  WK+EEA+KNLLRTHTTAVSARMLY+L+Q+++FTP KYFSI++VFRNE+LDATHLAEFHQVEG+IADRGL LA+L S +  FFK+LG DR++FKPAYNPYTEPSMEIFA+H GLGKWIE+GNSG+FRPEMLLPMGLP DVNVIAWGLSLERPTMIKY +N+IR+L+G K+DL  V  NPICRLD
Sbjct:  147 DNVRNDYKKRKLLQEVTIKSFLLSKGPQFATTIKKLETD-LTSEMLLTGAWKTLQFKPYNFDALGQPPECGHLHPLLKVRSEFREIFLEMGFNEMPTNQYIESSFWNFDALFQPQQHPARDAHDTFFMSSPTSTTEFPMEYLERVKKVHSEGGYGSQGYRYDWKIEEAQKNLLRTHTTAVSARMLYKLAQQEEFTPQKYFSIDKVFRNETLDATHLAEFHQVEGVIADRGLGLADLISVLDAFFKRLGFDRLQFKPAYNPYTEPSMEIFAYHTGLGKWIEIGNSGVFRPEMLLPMGLPEDVNVIAWGLSLERPTMIKYGLNNIRDLVGPKVDLQMVQNNPICRLD 490

HSP 2 Score: 90.8929 bits (224), Expect = 1.289e-15
Identity = 48/104 (46.15%), Postives = 69/104 (66.35%), Query Frame = 3 
Query:   39 ELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVVSEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKT-ANDKIGFAKAMSNKWI 347
             +L Y+ +N+   T+  +   N DHQ VVG +K+LE+ E VVSE     + ELT EGE V + GS+E  +Y+SIP NGI + +++KT  N K+GF+KAMS+ WI
Sbjct:    6 RILHYLEKNDKVDTIKLASEFNEDHQKVVGAVKSLEALEMVVSETTKSTRWELTGEGELVADKGSHEAVLYNSIPENGIPQAEVMKTVPNAKVGFSKAMSSGWI 109
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. TrEMBL
Match: E0D4V6 (Phenylalanyl-tRNA synthetase alpha subunit OS=Bombyx mori OX=7091 GN=FRSA PE=2 SV=1)

HSP 1 Score: 497.664 bits (1280), Expect = 1.881e-169
Identity = 230/341 (67.45%), Postives = 281/341 (82.40%), Query Frame = 2 
Query:  464 NDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            NDYKKRKL+++VT+K F++ KGP+F+ T  +     +T + +    + + +FKPYN    G      +LHPLLK+R E++ I +E+GF EMPTN YVESSFWNFDALFQPQ H ARDAHDTF++S P  + +FP EYLERV  VHSKGGYGSQGYR  WK+EEA+KNLLRTHTTAVSARMLYRL+Q+ +FTP KYFSI++VFRNE+LDATHLAEFHQVEG++ADRGL LA+L + +  FFK+LG D+++FKPAYNPYTEPSMEIFA+H GL KWIE+GNSG+FRPEMLLPMGLP DVNVIAWGLSLERPTMIKY +N+IR+L+G K+DL  V+ NPICRLD
Sbjct:  151 NDYKKRKLLQEVTIKSFLLSKGPQFAITIKKLETD-LTSDMLLSGSWKTLEFKPYNFDALGQPPECGHLHPLLKVRSEFREIFLEMGFTEMPTNKYVESSFWNFDALFQPQQHPARDAHDTFFMSSPAATTEFPMEYLERVKKVHSKGGYGSQGYRYDWKIEEAQKNLLRTHTTAVSARMLYRLAQQTEFTPQKYFSIDKVFRNETLDATHLAEFHQVEGVVADRGLGLADLITVLDAFFKRLGFDQLQFKPAYNPYTEPSMEIFAYHTGLAKWIEIGNSGVFRPEMLLPMGLPEDVNVIAWGLSLERPTMIKYGLNNIRDLVGPKVDLRMVYNNPICRLD 490

HSP 2 Score: 87.4261 bits (215), Expect = 1.390e-14
Identity = 46/104 (44.23%), Postives = 67/104 (64.42%), Query Frame = 3 
Query:   39 ELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVVSEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVK-TANDKIGFAKAMSNKWI 347
             +LKY+  ++   TL   +  N +HQ +VG +K+LE+ E V+SE     K ELTEEGE V N GS+E  +Y SIP +G+ + + +K   N K+GF+KAMS+ WI
Sbjct:    6 RILKYLENSDKADTLKLCEEFNEEHQKIVGAVKSLEALEMVISEAVKITKWELTEEGELVANNGSHEAVLYRSIPDSGVPQSEAMKMVPNAKVGFSKAMSSGWI 109
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. TrEMBL
Match: A0A437BLU6 (AA_TRNA_LIGASE_II domain-containing protein OS=Chilo suppressalis OX=168631 GN=evm_003861 PE=4 SV=1)

HSP 1 Score: 493.812 bits (1270), Expect = 5.747e-168
Identity = 228/346 (65.90%), Postives = 283/346 (81.79%), Query Frame = 2 
Query:  449 GDQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
             D + NDYKKRKL+++VT+K F++ KGP+F+TT  +    + TE  +    + + +FKPYN    G      +LHPLLK+R E++ I +E+GF EMPTN Y+ESSFWNFDALFQPQ H ARDAHDTF++ +P  +  FP +YLERV  VHS+GGYGSQGYR  WK+EEA+KNLLRTHTTAVSARMLY+L+Q+K+FTP KYFSI++VFRNE+LDATHLAEFHQVEG+IA RGL LA+L  T+  FF++LG DR++FKPAYNPYTEPSMEIFA+H GLGKWIE+GNSG+FRPEMLLPMGLP DV+VIAWGLSLERPTMIKY +++IR+L+G K+DL  V  NPICRL+
Sbjct:  146 ADNVRNDYKKRKLLQEVTIKSFMLSKGPQFATTIKKLETDLTTEMLLSG-SWKTLEFKPYNFDALGQPPECGHLHPLLKVRSEFREIFLEMGFTEMPTNQYIESSFWNFDALFQPQQHPARDAHDTFFILDPATTTDFPMDYLERVKKVHSEGGYGSQGYRYKWKIEEAQKNLLRTHTTAVSARMLYKLAQQKEFTPQKYFSIDKVFRNETLDATHLAEFHQVEGVIAGRGLGLADLVGTLDAFFRRLGFDRLQFKPAYNPYTEPSMEIFAYHTGLGKWIEIGNSGVFRPEMLLPMGLPEDVSVIAWGLSLERPTMIKYGLDNIRDLVGPKVDLQMVQTNPICRLN 490

HSP 2 Score: 89.3521 bits (220), Expect = 4.070e-15
Identity = 46/104 (44.23%), Postives = 69/104 (66.35%), Query Frame = 3 
Query:   39 ELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVVSEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTA-NDKIGFAKAMSNKWI 347
            ++L  + +N+   TL  +   N DHQ +VG +K+LE+ E V SE     + ELTEEG+QV   GS+E  +Y S+P +GI + +L+K+A N K+GF+KAMS+ WI
Sbjct:    6 KILHRLEKNDKVDTLQLASEFNEDHQKIVGAVKSLEALEMVTSEATKNTRWELTEEGQQVAANGSHEAVLYRSVPESGITQAELMKSAPNAKVGFSKAMSSGWI 109
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. TrEMBL
Match: S4PH52 (Phenylalanyl-tRNA synthetase alpha subunit OS=Pararge aegeria OX=116150 PE=4 SV=1)

HSP 1 Score: 489.189 bits (1258), Expect = 9.668e-167
Identity = 230/345 (66.67%), Postives = 278/345 (80.58%), Query Frame = 2 
Query:  452 DQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            D + +DYKKRKL++++T+K F++ KGPKF+T+  +     +T E +    +   +FKPYN    G      +LHPLLK+R E++ I +E+GF EMPTN YVESSFWNFDALFQPQ H ARDAHDTF++S P  S +FP +YLERV  VHS+GGYGSQGYR  WK+ EA+KNLLRTHTTAVSARMLYRL+Q+K+FT  KYFSI++VFRNESLDATHLAEFHQVEG+ A RGL LA+L  T+  FF +LG  R++FKPAYNPYTEPSMEIFAFH GLGKWIE+GNSG+FRPEMLLPMGLP DV+VIAWGLSLERPTMIKY +N+IR+L+G K+DL  V  NPICRLD
Sbjct:  103 DNIRSDYKKRKLLQEITLKSFVLTKGPKFATSIKKMETD-LTSEMLLTGAWKELQFKPYNFDALGQPPECGHLHPLLKVRSEFREIFLEMGFTEMPTNQYVESSFWNFDALFQPQQHPARDAHDTFFISSPATSTEFPVDYLERVKKVHSEGGYGSQGYRYSWKIAEAQKNLLRTHTTAVSARMLYRLAQQKEFTAQKYFSIDKVFRNESLDATHLAEFHQVEGVAAARGLGLADLVGTLDAFFARLGFRRLQFKPAYNPYTEPSMEIFAFHEGLGKWIEIGNSGVFRPEMLLPMGLPEDVSVIAWGLSLERPTMIKYGLNNIRDLVGPKVDLQMVQDNPICRLD 446
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Cavefish
Match: farsa (phenylalanyl-tRNA synthetase subunit alpha [Source:NCBI gene;Acc:103030421])

HSP 1 Score: 478.789 bits (1231), Expect = 2.469e-165
Identity = 216/336 (64.29%), Postives = 275/336 (81.85%), Query Frame = 2 
Query:  479 RKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            RKL+ +VT+K + + KG  FSTT  +     +T E I    +   KFKPYN    G+     +LHPL+K+R ++++I +E+GF EMPTNN++ESSFWNFD+LFQPQ H ARD HDTF++S+P  + +FPQ+YLERV  VHS+GGYGSQGY+  WK+EEA+KNLLRTHTTAVSARMLY+L+Q++KFTPVKYFSI+RVFRNE+LDATHLAEFHQ+EGL+ D GL+L +L   + +FF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP DV+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +P+CRLD
Sbjct:  162 RKLLSEVTIKSYWITKGRNFSTTITKQETE-LTPEMIASGSWRDKKFKPYNFEAMGVAPDSGHLHPLMKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDSLFQPQQHPARDQHDTFFMSDPALAREFPQDYLERVKKVHSEGGYGSQGYKYDWKIEEAQKNLLRTHTTAVSARMLYKLAQQEKFTPVKYFSIDRVFRNETLDATHLAEFHQIEGLVVDYGLTLGDLMGVLHQFFMKLGITKLRFKPAYNPYTEPSMEVFSYHEGLKKWVEVGNSGVFRPEMLLPMGLPEDVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPVCRLD 496

HSP 2 Score: 73.559 bits (179), Expect = 1.847e-13
Identity = 34/90 (37.78%), Postives = 59/90 (65.56%), Query Frame = 3 
Query:  102 NIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWIGFPRKTE 368
             ++HQ VVG +K+L++  +V+ +  +S    ELT EG ++   GS+E +++++IP  G+ +  L+K  + K+GF+KAMSNKWI   +  E
Sbjct:   31 GVEHQAVVGAVKSLQALGEVIEATQRSSKHWELTGEGREISEQGSHEARVFNAIPEEGLPQSQLMKLPSGKVGFSKAMSNKWIRLDKAHE 120
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009374.1 (pep scaffold:Pmarinus_7.0:GL476376:147333:247965:1 gene:ENSPMAG00000008453.1 transcript:ENSPMAT00000009374.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 476.863 bits (1226), Expect = 2.058e-165
Identity = 217/345 (62.90%), Postives = 280/345 (81.16%), Query Frame = 2 
Query:  452 DQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            D+  N+ KKRKL+ +V VK + + KGP FST+  +     +T E I    +   KFKPYN    GI+    +LHPLLK+R ++++I +E+GF EMPTNN++ESSFWNFDALFQPQ H ARD HDTF+++EP  +  FP++YLERV  +HS GGYGSQGY+  WK EEA+KN++RTHTTAVSARMLY+L+Q++KFTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++AD GL+L +L   + +FF KLG+ ++RFKPA+NPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP  V+VIAWGLSLERPTMIKYRI++IREL+G K++L  V+ +PICRL+
Sbjct:  152 DKERNELKKRKLLTEVVVKTYWVSKGPAFSTSIIKQETD-LTPEMIASGSWRDLKFKPYNFDAMGIMPDSGHLHPLLKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDALFQPQQHPARDQHDTFFIAEPANATCFPEDYLERVKQIHSVGGYGSQGYKYEWKREEAQKNIMRTHTTAVSARMLYQLAQQEKFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADFGLTLGDLMGVLHQFFTKLGITKLRFKPAFNPYTEPSMEVFSYHEGLKKWVEVGNSGVFRPEMLLPMGLPEGVSVIAWGLSLERPTMIKYRIDNIRELVGHKVNLQMVYNSPICRLE 495

HSP 2 Score: 82.0333 bits (201), Expect = 6.156e-17
Identity = 41/110 (37.27%), Postives = 67/110 (60.91%), Query Frame = 3 
Query:   42 LLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVVS-EMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWIGFPRKTE 368
            LL  +  ++   +   +    +DHQ+VVG +K+L+S   V+  E +S  K EL EEG +V   GS+E +++ ++P  GI + +LVK  N K+GF+KAMS+KW+   +  E
Sbjct:   10 LLARLQASDGLDSGDVAAQFGVDHQMVVGAVKSLQSQGDVIQVENRSATKWELMEEGREVVEHGSHEARVFHAVPPEGILQSELVKLPNGKVGFSKAMSSKWLRMDKSAE 119
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Yeast
Match: FRS2 (Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar [Source:SGD;Acc:S000001872])

HSP 1 Score: 385.185 bits (988), Expect = 1.255e-129
Identity = 184/346 (53.18%), Postives = 249/346 (71.97%), Query Frame = 2 
Query:  455 QLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQE--YLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            ++LND KKRKLI    +  F + KGP+FST D       +T + +    +   KFKPYN +  G+      LHPL K+R+E+++I   +GF EMP+N YVE+ FWNFDAL+ PQ H ARD  DTFY+ +P  +D  P +  Y++ +  VH +G +GS GYR  WK EE +K +LRTH+TA+SARML+ L+++ K  P + FSI+RVFRNE++DATHLAEFHQVEG++AD  ++L +L   + EFF+++G+  +RFKP YNPYTEPSMEIF++H GL KW+E+GNSGMFRPEML  MGLP D+ V+ WGLSLERPTMIKY++ +IREL+G K+ L+ +  NP  RLD
Sbjct:  157 KILNDLKKRKLIAQGKITDFNVTKGPEFST-DLTKLETDLTSDMVSTNAYKDLKFKPYNFNSQGVQISSGALHPLNKVREEFRQIFFSMGFTEMPSNQYVETGFWNFDALYVPQQHPARDLQDTFYIKDPLTAD-LPDDKTYMDNIKAVHEQGRFGSIGYRYNWKPEECQKLVLRTHSTAISARMLHDLAKDPK--PTRLFSIDRVFRNEAVDATHLAEFHQVEGVLADYNITLGDLIKFMEEFFERMGVTGLRFKPTYNPYTEPSMEIFSWHEGLQKWVEIGNSGMFRPEMLESMGLPKDLRVLGWGLSLERPTMIKYKVQNIRELLGHKVSLDFIETNPAARLD 498
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Yeast
Match: MSF1 (Mitochondrial phenylalanyl-tRNA synthetase; active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase [Source:SGD;Acc:S000006251])

HSP 1 Score: 55.0694 bits (131), Expect = 1.265e-8
Identity = 32/89 (35.96%), Postives = 51/89 (57.30%), Query Frame = 2 
Query: 1199 RIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGL-PSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFA 1462
            ++R+  AY P+T PS EI  +  G  +W+E+   G+ R ++LL  G  PS+    A+GL L+R  M+ + I DIR L  +    +R F+
Sbjct:  275 KVRWINAYFPWTAPSWEIEVWWQG--EWLELCGCGLIRQDVLLRAGYKPSETIGWAFGLGLDRIAMLLFEIPDIRLLWSRDERFSRQFS 361
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Nematostella
Match: EDO34480 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SP79])

HSP 1 Score: 447.203 bits (1149), Expect = 1.910e-153
Identity = 208/344 (60.47%), Postives = 270/344 (78.49%), Query Frame = 2 
Query:  461 LNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQ--KSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            + + KKRKL+    +K F + KG  FS +  Q   A +T + +    +   +FK YN    G      +LHPL+K+R ++++I +E+GF EM TNN++ESSFWNFD+LFQPQ H ARDAHDTF++S+P+  K  + PQ Y+E V  VH +GGYGSQGY+  WK EEA+KNLLRTHTTAVSARMLY+L Q++KFTP K+FSI+RVFRNE+LDATHLAEFHQ+EGL+ADR L+L +L  T+ +FF+KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEML PMGLP DV+VIAWGLSLERPTMIKY I++IR+L+G K+DL+ V+ NPIC L+
Sbjct:  156 IKELKKRKLVTTQVIKSFYVTKGKAFSLSV-QRLEADLTADMLLSGSWRDKEFKAYNFDALGTPVTSGHLHPLMKVRAQFRQIFLEMGFTEMATNNFIESSFWNFDSLFQPQQHPARDAHDTFFISDPKFAKLGEVPQSYVEDVKRVHEQGGYGSQGYQYEWKTEEAKKNLLRTHTTAVSARMLYKLGQQEKFTPAKFFSIDRVFRNETLDATHLAEFHQIEGLVADRNLTLGDLIGTLHQFFRKLGITKLRFKPAYNPYTEPSMEVFSYHEGLKKWVEVGNSGVFRPEMLRPMGLPDDVSVIAWGLSLERPTMIKYGISNIRDLIGHKVDLDMVYRNPICLLE 498

HSP 2 Score: 63.5438 bits (153), Expect = 8.059e-11
Identity = 35/106 (33.02%), Postives = 62/106 (58.49%), Query Frame = 3 
Query:   63 NENTSTLTFSKNNNIDHQVVVGILKNLESTEKVV-SEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGI---NRDDLVKTANDKIGFAKAMSNKWIGFPRKTE 368
            N    T   ++    DHQ++VG++K+++S  +++ +E +    L LT+EG Q+   GS+E  ++ +IP  G+   N   L + AN  IGF+KAMS+ W+   ++ E
Sbjct:   17 NGGVDTFELAREFGKDHQIIVGLVKSIQSAGELINTEQRQVQSLVLTDEGNQIAENGSHEAWVFDAIPEGGMPQSNIKSLGEHAN--IGFSKAMSSGWLRIDKQAE 120
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Medaka
Match: farsa (phenylalanyl-tRNA synthetase subunit alpha [Source:NCBI gene;Acc:101166476])

HSP 1 Score: 484.952 bits (1247), Expect = 5.378e-167
Identity = 225/358 (62.85%), Postives = 288/358 (80.45%), Query Frame = 2 
Query:  425 EQLENFQTGDQL----LNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            E+L   ++G QL     N+ KKRKL+ +VTVK + + +G  FSTT  +     +T E I    +   KFKPYN    G+     +LHPL+K+R ++++I +E+GF EMPTNN++ESSFWNFD+LFQPQ H ARD HDTF+LS+P  + +FPQ+YLERV  VHS+GGYGSQGY+  WK+EEA+KN+LRTHTTAVSARMLY+L+Q++KFTPVKYFSI+RVFRNE+LDATHLAEFHQ+EG++AD GL+L +L   + +FF KLG+ ++RFKPAYNPYTEPSME+F++H GL KW+EVGNSG+FRPEMLLPMGLP DV+VIAWGLSLERPTMIKY IN+IREL+G K++L  V+ +PICRLD
Sbjct:  193 EKLLLVRSGAQLEEKDKNELKKRKLLTEVTVKSYWITRGSSFSTTLTKQETE-LTPEMIATGSWKDRKFKPYNFEAMGVAPDCGHLHPLMKVRTQFRQIFLEMGFSEMPTNNFIESSFWNFDSLFQPQQHPARDQHDTFFLSDPALAHEFPQDYLERVKTVHSEGGYGSQGYKYDWKMEEAQKNILRTHTTAVSARMLYKLAQQEKFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADYGLTLGDLMGVLHQFFTKLGITKLRFKPAYNPYTEPSMEVFSYHEGLKKWVEVGNSGVFRPEMLLPMGLPEDVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPICRLD 549

HSP 2 Score: 87.4261 bits (215), Expect = 6.852e-18
Identity = 44/108 (40.74%), Postives = 73/108 (67.59%), Query Frame = 3 
Query:   30 SVLELLKYINENEN-TSTLTFSKNNNIDHQVVVGILKNLESTEKVVS-EMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI 347
            SV  LL+++ + +    +L  + +  +DHQV+VG +K+L+S   V+S E +S    ELT+EG ++   GS+E +++ SIP+ G+ + +L+K +  KIGF+KAMSNKWI
Sbjct:   61 SVEALLQHVEKADGGVDSLDVASSIGVDHQVIVGAVKSLQSLGDVISAEQRSSKHWELTDEGTELAEQGSHEARVFGSIPLEGLPQSELMKLSFGKIGFSKAMSNKWI 168
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Medaka
Match: farsa (phenylalanyl-tRNA synthetase subunit alpha [Source:NCBI gene;Acc:101166476])

HSP 1 Score: 88.9669 bits (219), Expect = 2.178e-27
Identity = 47/119 (39.50%), Postives = 76/119 (63.87%), Query Frame = 3 
Query:   30 SVLELLKYINENEN-TSTLTFSKNNNIDHQVVVGILKNLESTEKVVS-EMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWI-------GFPR 359
            SV  LL+++ + +    +L  + +  +DHQV+VG +K+L+S   V+S E +S    ELT+EG ++   GS+E +++ SIP+ G+ + +L+K +  KIGF+KAMSNKWI       G PR
Sbjct:    6 SVEALLQHVEKADGGVDSLDVASSIGVDHQVIVGAVKSLQSLGDVISAEQRSSKHWELTDEGTELAEQGSHEARVFGSIPLEGLPQSELMKLSFGKIGFSKAMSNKWIRVDKSHEGGPR 124

HSP 2 Score: 53.5286 bits (127), Expect = 2.178e-27
Identity = 35/91 (38.46%), Postives = 50/91 (54.95%), Query Frame = 2 
Query:  425 EQLENFQTGDQL----LNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG-GIVNRDANLHPLLK 682
            E+L   ++G QL     N+ KKRKL+ +VTVK + + +G  FSTT  +     +T E I    +   KFKPYN    G+     +LHPL+K
Sbjct:  139 EKLLLVRSGAQLEEKDKNELKKRKLLTEVTVKSYWITRGSSFSTTLTKQETE-LTPEMIATGSWKDRKFKPYNFEAMGVAPDCGHLHPLMK 228
[Close]
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Planmine SMEST
Match: SMESG000051669.1 (SMESG000051669.1)

HSP 1 Score: 759.985 bits (1961), Expect = 0.000e+0
Identity = 378/381 (99.21%), Postives = 379/381 (99.48%), Query Frame = 2 
Query:  347 WV--SEKDGVKLIXXXXXXXXXXXXXXLEQLENFQTGDQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHGGIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 1483
            W+  SEKDGVKLIVKKSDNVVDKVKLDLEQLENFQTGDQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHGGIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD
Sbjct:  112 WIGFSEKDGVKLIVKKSDNVVDKVKLDLEQLENFQTGDQLLNDYKKRKLIKDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHGGIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQPQDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWKLEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDATHLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEPSMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPTMIKYRINDIRELMGQKMDLNRVFANPICRLD 492

HSP 2 Score: 228.024 bits (580), Expect = 0.000e+0
Identity = 114/118 (96.61%), Postives = 115/118 (97.46%), Query Frame = 3 
Query:    9 MSTPIVPSVLELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLESTEKVVSEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTANDKIGFAKAMSNKWIGFPRK 362
            MSTPIVPSVLELLKYINENENTSTLTFSKN NIDHQVVVGILKNLESTEKVVSEMKSYNKLELTEEGEQVKNLGSYEFQI+SSIPINGINRDDLVKTANDKIGFAKAMSNKWIGF  K
Sbjct:    1 MSTPIVPSVLELLKYINENENTSTLTFSKNYNIDHQVVVGILKNLESTEKVVSEMKSYNKLELTEEGEQVKNLGSYEFQIFSSIPINGINRDDLVKTANDKIGFAKAMSNKWIGFSEK 118
The following BLAST results are available for this feature:
BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
FARSA3.182e-16061.27phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
FARSA1.581e-15961.27phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
FARSA1.116e-15167.93phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
FARSA1.732e-14260.90phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
FARSA2.280e-6870.80phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fars-11.921e-15564.26Phenylalanyl Amino-acyl tRNA Synthetase [Source:U... [more]
fars-11.921e-15564.26Phenylalanyl Amino-acyl tRNA Synthetase [Source:U... [more]
fars-11.287e-15464.26Phenylalanyl Amino-acyl tRNA Synthetase [Source:U... [more]
fars-11.287e-15464.26Phenylalanyl Amino-acyl tRNA Synthetase [Source:U... [more]
fars-25.399e-1225.09Phenylalanyl Amino-acyl tRNA Synthetase [Source:U... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
alpha-PheRS6.216e-15564.96gene:FBgn0030007 transcript:FBtr0071190[more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
farsa1.464e-16364.37phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
farsa1.438e-16362.76phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
farsa1.653e-16362.76phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
farsa2.042e-16362.76phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
farsa2.291e-16362.76phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
farsa3.575e-16362.76phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Farsa3.563e-16062.17phenylalanyl-tRNA synthetase, alpha subunit [Sourc... [more]
Farsa2.816e-15862.17phenylalanyl-tRNA synthetase, alpha subunit [Sourc... [more]
Fars21.143e-923.75phenylalanine-tRNA synthetase 2 (mitochondrial) [S... [more]
Fars21.143e-923.75phenylalanine-tRNA synthetase 2 (mitochondrial) [S... [more]
Fars21.143e-923.75phenylalanine-tRNA synthetase 2 (mitochondrial) [S... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q7SYV0|SYFAB_XENLA1.022e-16362.97Phenylalanine--tRNA ligase alpha subunit B OS=Xeno... [more]
sp|Q1JPX3|SYFA_DANRE1.105e-16264.37Phenylalanine--tRNA ligase alpha subunit OS=Danio ... [more]
sp|Q6AZG6|SYFAA_XENLA3.824e-16263.05Phenylalanine--tRNA ligase alpha subunit A OS=Xeno... [more]
sp|Q505J8|SYFA_RAT1.936e-16062.46Phenylalanine--tRNA ligase alpha subunit OS=Rattus... [more]
sp|Q9Y285|SYFA_HUMAN1.528e-15961.27Phenylalanine--tRNA ligase alpha subunit OS=Homo s... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2H1WLT81.329e-17269.21SFRICE_017812 OS=Spodoptera frugiperda OX=7108 GN=... [more]
A0A2A4JJB02.138e-17167.83AA_TRNA_LIGASE_II domain-containing protein OS=Hel... [more]
E0D4V61.881e-16967.45Phenylalanyl-tRNA synthetase alpha subunit OS=Bomb... [more]
A0A437BLU65.747e-16865.90AA_TRNA_LIGASE_II domain-containing protein OS=Chi... [more]
S4PH529.668e-16766.67Phenylalanyl-tRNA synthetase alpha subunit OS=Para... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
farsa2.469e-16564.29phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000009374.12.058e-16562.90pep scaffold:Pmarinus_7.0:GL476376:147333:247965:1... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
FRS21.255e-12953.18Alpha subunit of cytoplasmic phenylalanyl-tRNA syn... [more]
MSF11.265e-835.96Mitochondrial phenylalanyl-tRNA synthetase; active... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO344801.910e-15360.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
farsa5.378e-16762.85phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
farsa2.178e-2739.50phenylalanyl-tRNA synthetase subunit alpha [Source... [more]
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BLAST of phenylalanine--tRNA ligase alpha subunit-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000051669.10.000e+099.21SMESG000051669.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035483 ID=SMED30035483|Name=phenylalanine--tRNA ligase alpha subunit-like|organism=Schmidtea mediterranea sexual|type=transcript|length=1570bp
CTTTTACAATGAGTACTCCAATTGTACCTTCTGTTTTGGAGTTATTAAAA
TATATTAATGAAAATGAAAACACAAGTACTTTAACATTTTCAAAAAATAA
TAATATTGACCACCAGGTCGTGGTCGGTATTTTAAAAAATCTAGAATCAA
CTGAAAAAGTTGTTTCTGAAATGAAAAGCTACAACAAATTAGAACTAACT
GAAGAAGGTGAACAAGTTAAAAATTTGGGCAGTTATGAGTTTCAAATTTA
CAGTTCAATTCCAATTAATGGAATCAATAGAGATGATCTAGTGAAAACTG
CAAACGATAAAATAGGATTTGCAAAAGCAATGTCTAATAAATGGATTGGG
TTTCCGAGAAAGACGGAGTGAAACTTATCGTAAAAAAATCCGATAATGTT
GTGGATAAAGTGAAATTAGATTTAGAACAACTGGAAAACTTTCAAACCGG
AGATCAATTATTAAATGATTATAAAAAGCGAAAACTCATTAAAGATGTAA
CAGTAAAGTACTTCATTATGAGAAAGGGTCCGAAATTTTCCACAACTGAC
GATCAAGATGCAATAGCTGTGATAACAGAGGAAAATATCAAATGTCTGGA
TTTTAATAGCTGTAAATTCAAGCCTTACAATCTCCACGGTGGAATCGTAA
ATCGCGATGCCAATTTACATCCGCTTTTGAAAATGCGTAAAGAATACAAA
CGGATTTTAATTGAGCTGGGATTTATGGAGATGCCTACGAATAATTACGT
TGAGAGCAGTTTCTGGAATTTCGACGCTCTTTTCCAACCCCAGGATCACT
CAGCTCGTGATGCTCATGATACGTTCTATTTATCGGAACCGCAGAAATCC
GATCAATTTCCCCAGGAATATCTCGAACGGGTTTCCGACGTCCATTCGAA
AGGTGGCTATGGATCACAGGGTTACCGAAACCCCTGGAAACTAGAAGAAG
CTCGCAAAAACCTGCTGAGAACTCACACGACCGCCGTCAGTGCGAGAATG
CTCTATCGATTGTCACAGGAAAAGAAATTCACTCCCGTTAAATATTTCAG
CATTAATCGGGTGTTCCGGAACGAATCTCTCGATGCGACGCATCTCGCGG
AATTCCATCAAGTGGAAGGTTTGATCGCGGATCGCGGCCTCAGTCTAGCG
AATCTCCAATCGACAATTGCGGAATTCTTCAAGAAACTCGGGATGGATCG
GATCCGATTCAAGCCCGCCTACAATCCGTACACGGAACCAAGCATGGAGA
TATTTGCGTTCCATAACGGGCTGGGCAAATGGATTGAGGTTGGCAATTCC
GGCATGTTCCGCCCGGAAATGCTGCTGCCCATGGGGTTGCCTAGTGATGT
CAATGTTATCGCCTGGGGATTGTCCCTCGAGCGGCCCACCATGATTAAGT
ACCGAATCAATGACATACGAGAGCTCATGGGACAGAAAATGGATCTAAAT
CGGGTTTTTGCTAATCCAATTTGTCGGTTGGATTAGTGTTTTATTTACCG
CTTTAATAAATTGTTGACTTTTTGATTTTTCCTGGTGTTTTTATCATTAT
TTCTGATATTTGCTCGGTGG
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protein sequence of SMED30035483-orf-1

>SMED30035483-orf-1 ID=SMED30035483-orf-1|Name=SMED30035483-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=123bp
FTMSTPIVPSVLELLKYINENENTSTLTFSKNNNIDHQVVVGILKNLEST
EKVVSEMKSYNKLELTEEGEQVKNLGSYEFQIYSSIPINGINRDDLVKTA
NDKIGFAKAMSNKWIGFPRKTE*
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protein sequence of SMED30035483-orf-2

>SMED30035483-orf-2 ID=SMED30035483-orf-2|Name=SMED30035483-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=382bp
MDWVSEKDGVKLIVKKSDNVVDKVKLDLEQLENFQTGDQLLNDYKKRKLI
KDVTVKYFIMRKGPKFSTTDDQDAIAVITEENIKCLDFNSCKFKPYNLHG
GIVNRDANLHPLLKMRKEYKRILIELGFMEMPTNNYVESSFWNFDALFQP
QDHSARDAHDTFYLSEPQKSDQFPQEYLERVSDVHSKGGYGSQGYRNPWK
LEEARKNLLRTHTTAVSARMLYRLSQEKKFTPVKYFSINRVFRNESLDAT
HLAEFHQVEGLIADRGLSLANLQSTIAEFFKKLGMDRIRFKPAYNPYTEP
SMEIFAFHNGLGKWIEVGNSGMFRPEMLLPMGLPSDVNVIAWGLSLERPT
MIKYRINDIRELMGQKMDLNRVFANPICRLD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR040725PheRS_DBD3
IPR040724PheRS_DBD1
IPR036390WH_DNA-bd_sf
IPR011991WHTH_DNA-bd_dom
IPR002319Phenylalanyl-tRNA_Synthase
IPR006195aa-tRNA-synth_II
Vocabulary: molecular function
TermDefinition
GO:0005524ATP binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0000049tRNA binding
GO:0000166nucleotide binding
GO:0004826phenylalanine-tRNA ligase activity
GO:0016874ligase activity
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0009328phenylalanine-tRNA ligase complex
Vocabulary: biological process
TermDefinition
GO:0043039tRNA aminoacylation
GO:0006412translation
GO:0006432phenylalanyl-tRNA aminoacylation
GO:0051290protein heterotetramerization
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040725PheRS, DNA binding domain 3PFAMPF18553PheRS_DBD3coord: 77..121
e-value: 6.5E-12
score: 45.6
NoneNo IPR availableGENE3DG3DSA:1.10.10.2310coord: 6..77
e-value: 3.6E-8
score: 35.4
NoneNo IPR availableGENE3DG3DSA:1.10.10.2330coord: 78..122
e-value: 8.9E-14
score: 52.7
NoneNo IPR availablePANTHERPTHR11538:SF40PHENYLALANINE--TRNA LIGASE ALPHA SUBUNITcoord: 24..118
NoneNo IPR availablePANTHERPTHR11538PHENYLALANYL-TRNA SYNTHETASEcoord: 24..118
IPR040724PheRS, DNA binding domain 1PFAMPF18552PheRS_DBD1coord: 13..57
e-value: 4.2E-10
score: 39.2
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILYSSF46785"Winged helix" DNA-binding domaincoord: 10..72