G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30035471
Length (bp)1454
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30035471) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30035471) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30035471) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30035471) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035471

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
muscle cellSMED30035471h1SMcG0010906 dd_Smed_v4_19759_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30035471h1SMcG0010906 dd_Smed_v6_19759_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: K03H6.1 (pep chromosome:WBcel235:IV:1523718:1526599:1 gene:WBGene00019367.1 transcript:K03H6.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:K03H6.1)

HSP 1 Score: 56.9954 bits (136), Expect = 1.736e-8
Identity = 61/230 (26.52%), Postives = 102/230 (44.35%), Query Frame = 1 
Query:  511 MTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTHVTKQFNKTNLI----HSGECTAKPQFHYFYRVW--------------TILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTH-----HLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQ 1131
            ++IER +I+  P RAK   Q+     V+SS  + + L+  +  F  D  + +  N T L+    +SG    + Q  Y  + W              T L+A L   +P  ++I LN+L++R+          L  N+     +S++   T +H      ++VT+IA+   F +T  P     L   F +  E+ H     F   I   L+ T  +INF+L+C +   FRQ
Sbjct:  116 VSIERYLIVRSPFRAKLYWQRGKMVVVLSSIFVTTGLLTVYHHFEWDCVIEEFCNGTQLLDFCYYSG---MRHQKTYRNKDWVTPSNVKKVYLKFSTFLNAILVVFVPIVLVIVLNMLMIRQ----------LKINWTSPRLESMRGAMTRSHVRQRQRVAVTVIAIGLCFSLTQGPSAFMALYELFSEY-ELGHTFYAIF--SITNSLVVTGKTINFILFCLSSEHFRQ 329
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: dmsr-7 (DroMyoSuppressin Receptor related [Source:UniProtKB/TrEMBL;Acc:O16548])

HSP 1 Score: 50.447 bits (119), Expect = 1.867e-6
Identity = 37/135 (27.41%), Postives = 70/135 (51.85%), Query Frame = 1 
Query:  733 YRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQAC--SKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQ 1131
            + +W I    L+ +IP  +LI L+  +V +I  ++ +R  L +  + A   SK +KK +  +   ++ L+ +  +FLIT  P  +  +L      D   H  +  ++  + ++L   N S+NF+LYC   +R+RQ
Sbjct:  224 WNIWMI--GILFKIIPCILLIFLSFGLVSKIRDAEKHRRKLTSVPSNASTDSKPLKKKNGTSDRTTLMLVVILLVFLITEFPQGIISILCAIFTTD--VHRYLYFYIGDVLDLLSLVNSSVNFVLYCVMSSRYRQ 354
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: ghsrb (growth hormone secretagogue receptor b [Source:ZFIN;Acc:ZDB-GENE-090327-1])

HSP 1 Score: 50.8322 bits (120), Expect = 1.834e-6
Identity = 71/296 (23.99%), Postives = 131/296 (44.26%), Query Frame = 1 
Query:  277 NVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVI---SSTIICSSLVASHFFFTVDTHVTKQFNKTNLIHSGECTAKPQFHYFYR-------VWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLI-HFRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQ 1131
            N+L  +V+  Y   + ++ +YL  M + D +I +   L  Y   ++     N     C+L QF+  S +  ++  I  +++ER   I  PLRAK I  +   + VI    +  +CS   A   F  V     +  N TN   + EC A     Y  R       VW    +S++  +P   L  L  LI R + R K N    +++ +++  +++K        L+V ++A    F++   P  V + L+    + +    + I ++ +++  +L Y + +IN +LY     +FR 
Sbjct:   60 NLLTILVVTKYKDMRTTTNLYLCSMALSDLLIFLCMPLDLY--RVWRYRPWNFGDELCKLFQFVSESCTYSTILNITALSVERYFAICFPLRAKVIVTRGRVKGVILLLWTVALCS---AGPIFILVG---VEHENGTNAWETNECKAT---EYAIRSGLLTMMVWV---SSVFFFLPVLCLTVLYSLIGRRLWRRKENPVGPISSRDKSNKQTVK-------MLAVVVLA----FVLCWLPFHVGRYLVSKSSEANSPVISQISEYCNLVSFVLFYLSAAINPILYNIMSKKFRS 330
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: ghsra (growth hormone secretagogue receptor a [Source:ZFIN;Acc:ZDB-GENE-070912-238])

HSP 1 Score: 50.0618 bits (118), Expect = 3.636e-6
Identity = 75/292 (25.68%), Postives = 123/292 (42.12%), Query Frame = 1 
Query:  277 NVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTHVTKQFNKTNLIHSGECTAKPQFHYFYR-------VWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNGIIQ-FLDVIFEMLMYTNHSINFLLYCATGNRFR 1128
            N++  +V+  Y   + ++ +YL+ M   D +I +   L  Y   I+     N   + C+L QF+    +  ++  I  +++ER   I  PLRAK +  K   R VI    I S   A   F  V     +  N TN   + EC A     Y  R       VW    +S++  +P   L  L  LI R++ + K  R  +  N     + S  K +  T  +   L  V F F++   P  V + LI    +       II  + ++I  +L Y + +IN +LY     ++R
Sbjct:   55 NLMTILVVTKYKDMRTTTNLYLSSMAFSDLLIFLCMPLDLY--RIWRYRPWNFGNILCKLFQFVSECCTYSTILNITALSVERYFAICFPLRAKVVVTKGRVRGVILVLWIVSFFSAGPVFVLVG---VEHENGTNSWDTNECKAT---EYAIRSGLLTIMVWV---SSIFFFLPVFCLTVLYSLIGRKLWKRK--RETIGEN-----ASSRDKSNRQTVKM---LAVVVFAFVLCWLPFHVGRYLISKSTEMGSPVMSIISHYCNLISFVLFYLSAAINPILYNIMSKKYR 325
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: ntsr1 (neurotensin receptor 1 (high affinity) [Source:ZFIN;Acc:ZDB-GENE-090313-372])

HSP 1 Score: 49.2914 bits (116), Expect = 6.675e-6
Identity = 59/272 (21.69%), Postives = 120/272 (44.12%), Query Frame = 1 
Query:  337 YLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTH-VTKQFNKTNLIHSGECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILR-----SKNNRSILMNNFNQACSKSIKKVSTDT-HHLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQ 1131
            +LA + I D +ILV  +      +I++ H        C+   F+    S  + + I  +++ER + I  P +AK I  +   + +IS+  I S  +A+   FT+    +  ++  T ++ S   TAK            L+A L  ++P  ++ +LN +I  ++LR     +++NR  ++       S +++     +  H  + L AV   F++   P    +L+  +  +          +  ++  +L Y + +IN +LY      +RQ
Sbjct:   94 HLASLAISDLLILVLCMPIELYNFIWVHHPWAFGEAVCKGYYFLRDGCSYATAFNIASLSVERFMAICHPFKAKSIMSRSRTKKLISAMWIASFFLATPMLFTMGQQLMNDEYICTTIVSS--ITAKTVLQ--------LNAFLSFVVPMALISALNGVIASQLLRMFRESAQDNRVCIIGGNATMLSVAVEPNRAQSMRHGVMVLRAVVIAFVVCWLPYHARRLMYCYVTEWTTALYDFYHYFYMVTNVLFYVSSAINPILYNLVSANYRQ 355
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: ntsr1 (neurotensin receptor 1 (high affinity) [Source:ZFIN;Acc:ZDB-GENE-090313-372])

HSP 1 Score: 49.2914 bits (116), Expect = 6.675e-6
Identity = 59/272 (21.69%), Postives = 120/272 (44.12%), Query Frame = 1 
Query:  337 YLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTH-VTKQFNKTNLIHSGECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILR-----SKNNRSILMNNFNQACSKSIKKVSTDT-HHLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQ 1131
            +LA + I D +ILV  +      +I++ H        C+   F+    S  + + I  +++ER + I  P +AK I  +   + +IS+  I S  +A+   FT+    +  ++  T ++ S   TAK            L+A L  ++P  ++ +LN +I  ++LR     +++NR  ++       S +++     +  H  + L AV   F++   P    +L+  +  +          +  ++  +L Y + +IN +LY      +RQ
Sbjct:   94 HLASLAISDLLILVLCMPIELYNFIWVHHPWAFGEAVCKGYYFLRDGCSYATAFNIASLSVERFMAICHPFKAKSIMSRSRTKKLISAMWIASFFLATPMLFTMGQQLMNDEYICTTIVSS--ITAKTVLQ--------LNAFLSFVVPMALISALNGVIASQLLRMFRESAQDNRVCIIGGNATMLSVAVEPNRAQSMRHGVMVLRAVVIAFVVCWLPYHARRLMYCYVTEWTTALYDFYHYFYMVTNVLFYVSSAINPILYNLVSANYRQ 355
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: mlnr (motilin receptor [Source:ZFIN;Acc:ZDB-GENE-130530-1015])

HSP 1 Score: 48.9062 bits (115), Expect = 7.743e-6
Identity = 64/279 (22.94%), Postives = 121/279 (43.37%), Query Frame = 1 
Query:  319 KCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTHVTKQFNKTNLIHSGECTAKPQFHYFYRVW------TILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHV 1137
            K ++ +YL+ M I D +I ++     Y  + ++  I  E V  CRL+ +I    +  ++  I V+++ER + I  P +AK    K+  + VI +    + L A+  FF +      ++    +   G    K   H  Y +       TI  ++ Y   P   L+ L   I R++ +S++     ++  N A  + + + +         L  V  +F I   P  + + L  F   D+ +   + Q  +V   +L Y + S+N +LY    N++R  V
Sbjct:   13 KTTTNLYLSSMAISDLVIFLSLPFDLYRLWKYVPWIFGEFV--CRLSHYINEGCTNATILHITVLSMERYLAICFPFKAKAAITKRRVKYVILALWGFALLSAAPMFFLMGV----EYENETMPDPGSRQCK---HTRYAIESGLLHTTIWVSTAYFFCPMFCLLFLYGSIGRKLWKSRHE----LHGPNAAARQKVNRQTVKI------LAVVVSVFAICWLPYHIGRFL--FTHVDDYHSARLSQNFNVASMVLFYLSASVNPVLYNLMSNKYRSAV 270
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: H2KNW0 (G-protein coupled receptor OS=Clonorchis sinensis OX=79923 GN=CLF_100861 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.775e-46
Identity = 122/360 (33.89%), Postives = 203/360 (56.39%), Query Frame = 1 
Query:  160 ILKETLMKEIPGKLLYSCRLYHIFWTXXXXXXXXXXXXXNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQ-ITQKKIARAVISSTIICSSLVASHFFFTVD-THVTK-------QFNKTNLIHSGE----------------------CTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIH---FRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHV 1137
            ++K  +M++ PG  L +C+++H  WT +PPI+ L+GS+GN LA +VL        S ++YL    +VDE++L+ GLLRR+I Y+    +EN S +FC+L QF+G SSSL+SVWL V++T ER +++  PL+  + +T  K+ RA++  +++C + ++ HFFFTVD  H+         + N ++++  G                       C+   +      +WT +DA +Y  +PFC++ SLN++I+  +  +  ++  +     +  S S+  V  +   L++ L  VSF FL+TT  +VV K LI     +    V +    Q  D + E+LMY NH++NF L+CATG RFR  +
Sbjct:   42 LIKTVIMRKSPGVFLTTCKIHHFLWTFVPPIVFLLGSLGNTLAFLVLRHPATRHVSVFVYLTARSVVDEIVLIVGLLRRWIDYLVGTKLENTSAIFCKLAQFLGTSSSLLSVWLTVLLTAERALVVTFPLQGNRLVTFAKVRRAIVFLSVVCVA-ISMHFFFTVDLVHLNAENCVLRVEMNGSSMLDEGSHEFLLNITSLKGKSTKSVCDGHCSFPDEMATMRWLWTTVDAIIYCYLPFCLIFSLNIVILHYVYAAHKDQYGIRAQPERLKSGSL-GVDNNIRQLTIMLQVVSFSFLVTTVSIVVMKTLIQVPAMKSVTSVQNKAAFQLADTVAELLMYINHAMNFYLFCATGKRFRSRL 399
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A1S8WXZ4 (7 transmembrane receptor OS=Opisthorchis viverrini OX=6198 GN=X801_04808 PE=4 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 7.853e-46
Identity = 127/361 (35.18%), Postives = 200/361 (55.40%), Query Frame = 1 
Query:  160 ILKETLMKEIPGKLLYSCRLYHIFWTXXXXXXXXXXXXXNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQ-ITQKKIARAVISSTIICSSLVASHFFFTVDTHVTKQ------------------------FNKTNL-------IHSGECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIH---FRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHV 1137
            ++K  +M++ PG  L +C++YH  WT +PPI+ L+GS+GN LA +VL        S ++YL    +VDE++L  GLLRR+I Y+    +EN S +FC+L QF+G SSSL+SVWL V++T ER +++  PL+  + +T  K+ RA+I  +++C + ++ HFFFTVD  V +                         FN T+L       +  G C+          +WT +DA +Y  +PFC++ SLN++I+  +  +  ++  +     +  S S+  V  +   L++ L  VSF FL+TT  +VV K LI     +    V      Q  D + E+LMY NH++NF L+CATG RFR  +
Sbjct:   42 LIKTVIMRKSPGVFLTTCKIYHFLWTFVPPIVFLLGSLGNTLAFLVLRHPATRHVSVFVYLTARSVVDEIVLTVGLLRRWIDYLVGTKLENTSAIFCKLAQFLGTSSSLLSVWLTVLLTAERALVVTFPLQGNRLVTFAKVRRAIIFLSVVCVA-ISMHFFFTVDL-VQRNAENCVLRVEMNGSSPLDEGSHEFLFNITSLKGKSSKSVCDGHCSFPDGMATMRWLWTTVDAIIYCYLPFCLIFSLNIVILHYVYAAHKDQYGIRAQPERLKSGSL-GVDNNIRQLTIMLQVVSFSFLVTTVSIVVMKTLIQVPAMKSVTSVQTKAAFQLADTVAELLMYINHAMNFYLFCATGKRFRSRL 399
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A4S2KQC3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_010711 PE=4 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 1.180e-45
Identity = 125/360 (34.72%), Postives = 200/360 (55.56%), Query Frame = 1 
Query:  160 ILKETLMKEIPGKLLYSCRLYHIFWTXXXXXXXXXXXXXNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQ-ITQKKIARAVISSTIICSSLVASHFFFTVD-THVTKQ----------------------FNKTNL-------IHSGECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIH---FRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHV 1137
            ++K  +M++ PG  L +C+++H  WT +PPI+ L+GS+GN LA +VL        S ++YL    +VDE++L  GLLRR+I Y+    +EN S +FC+L QF+G SSSL+SVWL V++T ER +++  PL+  + +T  K+ RA++  +++C + V+ HFFFTVD  H+  +                      FN T+L       +  G C+          +WT +DA +Y  +PFC++ SLN++I+  +  +  ++  +     +  S S+  V  +   L++ L  VSF FL+TT  +VV K L      +    V      Q  D + E+LMY NH++NF L+CATG RFR  +
Sbjct:   42 LIKTVIMRKSPGVFLTTCKIHHFLWTFVPPIVFLLGSLGNTLAFLVLRHPATRHVSVFVYLTARSVVDEIVLTVGLLRRWIDYLVGTKLENTSAIFCKLAQFLGTSSSLLSVWLTVLLTAERALVVTFPLQGNRLVTFAKVRRAIVFLSVVCVA-VSMHFFFTVDLVHLNAENCVLRVEMNGSSMLDDGSHEFLFNITSLKGKSAKSVCDGHCSFPDGMATMRWLWTTVDAIIYCYLPFCLIFSLNIVILHYVYAAHKDQYGIRAQPERLKSGSL-GVDNNIRQLTIMLQVVSFSFLVTTVSIVVMKTLTQVPAMKSVTSVQTKAAFQLADTVAELLMYINHAMNFYLFCATGKRFRSRL 399
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A504YW10 (G-protein coupled receptor OS=Fasciola gigantica OX=46835 GN=FGIG_06654 PE=4 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 9.570e-37
Identity = 121/355 (34.08%), Postives = 185/355 (52.11%), Query Frame = 1 
Query:  208 SCRLYHIFWTXXXXXXXXXXXXXNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQ-ITQKKIARAVISSTIICSSLVASHFFFTVD-------THVTKQFNKTNLIHSG--------------------------ECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNR--------SILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIHFRD---KDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHV 1137
            +C  Y   WT IPPII+L+GS GN+L+ +VL        S+++YL V  +VDE++L+ GLLRR+I Y+  + +E+ S   C++ QF GVSSS +SVWL V +T ER +++  PL   + IT  ++ R + + T+IC  L+++HFF TVD       T   K+   + L                               C  KP+F     +WT LDA +Y  +PF  +  LNV+IV     ++  +        +I M    +  S S ++V+ +   +++ L+ +SF FLITT  +VV K+L    D             Q  D + E+LMY NH++NF L+CATG RFR  +
Sbjct:   61 ACSAYRFLWTFIPPIIILLGSFGNILSFLVLRHPSTRYVSAFVYLTVRSVVDEVVLIVGLLRRWIDYLINRKLEDTSSTLCKMAQFAGVSSSFLSVWLTVTLTAERALVVSFPLHGTRFITYSRVRRLIFTLTVICV-LLSTHFFITVDLIPIPGSTKAKKEHAYSELWEQDANRTVLQRNYSQPELSTIMPTSEQVYRCNFKPEFAALAWLWTTLDAVIYCYLPFIFIFGLNVVIVHHANAAQKEQHRIRVRHSTISMQQLGKTPSFSREQVN-NVRQMTIMLLVISFAFLITTLAIVVTKILSQVLDLSGSSRRLQRLNFQLADSVAELLMYINHAMNFYLFCATGKRFRSRL 413
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183A9A8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS3543 PE=4 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 1.885e-36
Identity = 122/363 (33.61%), Postives = 188/363 (51.79%), Query Frame = 1 
Query:  202 LYSCRLYHIFWTXXXXXXXXXXXXXNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVD-------------------------------THVTKQF-NKTNLIHSG-----------ECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREI--LRSKNNR------SILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIHFRD---KDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFR 1128
            L +C  Y   WT IPPII+L+GS GN+LA +VL        S+++YL V  +VDE++L  GLLRR+I Y+  + +E+ + +FC+L QF GVSSS +SVWL V +T ER +++  PL   +       R +I    +   L++ HFF TVD                               ++ T+ + N+T    +G           +C  KP+F  F  +WT +DA +Y  +PF ++  LNV+IV  +   + K +R       I M   ++A   S  +++ +   +++ L+ VSF FL+TT  +VV K+L    D       +     Q  D + E+LMY NH++NF L+CATG RFR
Sbjct:   59 LPACSAYRFLWTYIPPIIILLGSFGNILAFMVLRHPSTRYVSAFVYLTVRSVVDEVVLTVGLLRRWIDYMINRKLEHSTSVFCKLAQFAGVSSSFLSVWLTVTLTAERALVVSFPLHGTRFISYSRVRKLIIVLSLICILLSLHFFITVDLIPTTTTPSSSTATTDFIHQPYHSLYLEPQDSNTTEPWRNQTQSAITGDVSSVRQEMVYQCNFKPEFASFAWLWTTIDAVVYCYLPFILIFGLNVVIVYHVNAAQKKQHRIRVRHSVIPMQQLSKATGASGDQMN-NVRQMTIMLLVVSFAFLVTTVAIVVTKILSQVLDLSGSGRTHERLYFQLADTVAELLMYINHAMNFYLFCATGKRFR 420
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Match: nmur3 (neuromedin-U receptor 2-like [Source:NCBI gene;Acc:103035443])

HSP 1 Score: 49.6766 bits (117), Expect = 4.754e-6
Identity = 65/299 (21.74%), Postives = 132/299 (44.15%), Query Frame = 1 
Query:  277 NVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGL-LRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICS--------SLVASHFFFTVDTHVTKQFNKTNLIHSGECT-AKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRS-ILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIHF--RDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQH 1134
            N+L CIV+  +   +  + +YL  + + D ++L+ G+ L  Y  +        ESV  C    F+  +    SV  + V+++ER + +  PL+ +     K A+ +I    I S        SL   H+++  +           ++ S  C   KPQ  + Y +   +   L+  +P  V+  L ++I  ++ R +  +   L  N +   S+ ++  S     ++  L  V  +F I   P    +LL  F  +  D +Y   + +++ ++  +L Y + ++N ++Y    +RFR+ 
Sbjct:   74 NLLTCIVIIKHRKMRTPTNLYLFSLAVSDLLVLLFGMPLEIYELWQNYPFPFGESV--CCFKVFLFETVCFASVLNVTVLSVERYVAVVHPLKTRYALTNKHAQKMIGGVWIVSLVCAVPNTSLHGIHYYYLPE----------KVLESATCNLLKPQ--WIYNLVIQVTTVLFYFVPMMVICGLYLVIGMKLGREQKQQGRKLGRNCSADVSRRVQVESGRRRQVTKMLAVVVLVFAICWAPFHTDRLLWSFITQWTDRMY--SVFEYVHILSGVLFYLSSAVNPIIYNLLSSRFRER 356
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Match: nmur1a (neuromedin U receptor 1a [Source:ZFIN;Acc:ZDB-GENE-081104-220])

HSP 1 Score: 48.1358 bits (113), Expect = 2.708e-6
Identity = 65/303 (21.45%), Postives = 127/303 (41.91%), Query Frame = 1 
Query:  277 NVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGL-LRRYIGYIFIKHIENESVLF----CRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVIS----STIICSSLVAS-HFFFTVDTHVTKQFNKTNLIHSGECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKN--------NRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQ 1131
            N L CIV+  + + +  +  YL  + + D ++LV G+ L  Y      +  EN   LF    C     +  +    SV  +  +++ER I +  PL+A+ +  +  AR VI+    +++ CS    S H    + + + + + ++ +     C    Q  + Y V   L A  + L+P   +  L +LI R++ R K+         R    N       + I+   +    ++  L  +  +F I   P    +L+  F  +     +G+ Q + ++  +  Y +  +N ++Y     RFR 
Sbjct:   71 NFLTCIVISRHRLMRTPTNFYLFSLAVSDLLVLVLGMPLELY------EMWENYPSLFGEGGCYFKTMLFETVCFASVLNVTALSVERYIAVVHPLKARWLVTRTHARRVIACLWVASVCCSVPNTSLHGIHYIRSPLGEPYEESTI-----CIVT-QPRWIYNVVVQLTALFFFLVPMATISVLYLLIGRQLRREKSLAVPHECEGRGTFGN-------QRIRTNRSKCRQVTKMLYVLVIVFGICWAPFHTDRLVWSFVHRWTADSHGMFQCVHLVSGVFFYLSSVVNPVIYNLLSTRFRD 354
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000029588.1 (neuromedin-U receptor 1-like [Source:NCBI gene;Acc:101166005])

HSP 1 Score: 50.447 bits (119), Expect = 2.633e-6
Identity = 46/215 (21.40%), Postives = 96/215 (44.65%), Query Frame = 1 
Query:  511 MTIERVIIIYLPLRAKQITQKKIARAVI----SSTIICSSLVAS-HFFFTVDTHVTKQFNKTNLIHSGECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMN-NFNQACSKSIKKVSTDTHHLSVT--LIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQ 1131
            +++ER + +  PL+ K +  +  AR VI      +++C+    S H    +     ++F   +   S  C A+P   + Y +  ++   ++ ++P  V+  L +LI   + R K   ++  N +F         +      +L VT  L  +  +F +   P  V +L+  + DK  + H  I + + ++  +  Y + ++N +LY     RFR+
Sbjct:  163 LSVERYVAVTEPLKVKHVATRAHARRVIFMLWGLSMLCALPNTSVHGIKVLSPRFGREF--PSFSSSAGCLAEPD--WMYNLIILISTLVFFMLPMLVISILYLLIGLRLRREKLMATMDANHSFGPGSISKSHRQRLTKRNLQVTKMLCVLVVVFGLCWAPFHVDRLMWSYIDKSSIQHQKIFEKVHIVSGVFFYLSSAVNPILYNLMSTRFRE 373
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000061235.1 (SMESG000061235.1)

HSP 1 Score: 733.791 bits (1893), Expect = 0.000e+0
Identity = 414/417 (99.28%), Postives = 416/417 (99.76%), Query Frame = 1 
Query:   22 MSSTNICNNVESDGANTAXXXXXXXXXXXXPYASSTSNLSVLEFRLILKETLMKEIPGKLLYSCRLYHIFWTXXXXXXXXXXXXXNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTHVTKQFNKTNLIHSGECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHVXXXXXXXXXXXNRPADNTKCARSTIQISEMDAMLRGKRFTVYQEE 1272
            MSSTNICNNVESDGANTALFLLSLNDSLLLPYASSTSNLSVLEFRLILKETLMKEIPGKLLYSCRLYHIFWT+IPPIILLIGSIGNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGVSSSLVSVWLIVVMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTHVTKQFNKTNLIH GECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYH+GIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHVLSLLLCRTLSLNRPADNTKCARSTIQISEMDAMLRGKRFTVYQEE
Sbjct:    1 MSSTNICNNVESDGANTALFLLSLNDSLLLPYASSTSNLSVLEFRLILKETLMKEIPGKLLYSCRLYHIFWTVIPPIILLIGSIGNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGVSSSLVSVWLIVVMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTHVTKQFNKTNLIHFGECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHSGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHVLSLLLCRTLSLNRPADNTKCARSTIQISEMDAMLRGKRFTVYQEE 417
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000053893.1 (SMESG000053893.1)

HSP 1 Score: 194.126 bits (492), Expect = 1.291e-56
Identity = 143/355 (40.28%), Postives = 213/355 (60.00%), Query Frame = 1 
Query:  115 YASSTSNLSVLEFRLILKETLMKEIPGKLLYSCRLYHIFWTXXXXXXXXXXXXXNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTHVTKQFNKTNLIHS-GECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSIL-----MNNFNQACSKSIKKVST-------DTHHLSVTLIAVSFIFLITTTPLVVHKLLIH-FRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHV 1137
            + + + NL++ E R  L++ L+++IPG  L  C + +  WT +PP ILL G+ GN+LA  V+C   + K SSY+YLA + I+DE+IL+TGLLRR+  ++    IE+ +   C++ QFIGV+ S  SVWLIV MT+ER+III LPL+  ++      +  I   I C SL++SHFF TV  +     N   +      C  K ++++F R W + +A  YS+IPF +LI  N +I+    ++   RS L     M    Q+ S + +KV T       +T  L+V L+ +S  FL TTTPLV +KL++    + D+V +  I Q +D IFE+LMY NHSINF +YCA+G RFR  +
Sbjct:   34 FHNESQNLNIREIRNKLEQLLVEKIPGSKLLHCVILNNLWTFLPPFILLFGTFGNILAFFVICQRHLLKTSSYVYLAAIAIIDELILLTGLLRRWSDHLVGSRIEDSNKFSCKIFQFIGVTVSFSSVWLIVFMTVERLIIIKLPLKVNRLVSPNKVKVYIVILIFCLSLLSSHFFETVSLNNQNNSNSNLVTTPLYRCNFKDKYYHFIRYWMMFEALFYSIIPFILLIIFNGIILSLTYKASKTRSTLVKQCYMTRQFQSISHNRQKVVTNVSQLNHETKQLTVMLVTISVAFLCTTTPLVTYKLILKVINNTDDVRYLAIRQLMDTIFELLMYINHSINFYIYCASGRRFRTQL 388
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042142.1 (SMESG000042142.1)

HSP 1 Score: 190.66 bits (483), Expect = 7.064e-55
Identity = 150/365 (41.10%), Postives = 215/365 (58.90%), Query Frame = 1 
Query:  133 NLSVLE---FRLILKETLMKEIPGKLLYSCRLYHIFWTXXXXXXXXXXXXXNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVD------THVTKQFNKTNLIHS-GECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMN---NFNQACSKSI---------KKVST---DTHHLSVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNG-----IIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHV 1137
            NL++ E   +R  L   L+K+IPGKL+  C  ++  W +IP  I+ +GSIGN L+ +VLC   +   SSYIYLA + I DE+IL+ GL R++I ++    +EN S+ FC+L Q IGVSSS +SVWLIV +T+ERV+II  P  A +  +   A+ +I S II S   + HFF TV        +V   F+     +S  +C  K ++  F   W  +D + YS IP  +LI  N++I+ EI +++N  + ++N   + N+    SI         K+ S+   +T  L+V L+ VSF FLITT+P V+ KLLI  +     Y NG      +  LD IFEMLMY NH+INF LYCATGN+FRQ +
Sbjct:   35 NLTMSELPAYRRRLFNILVKKIPGKLITECIFHNWLWMLIPIFIVAMGSIGNFLSAVVLCRKSMSHISSYIYLATLAIFDELILLCGLFRKWIEHVTDWSLENHSISFCKLFQVIGVSSSFISVWLIVTLTVERVLIIKRPFEASKYAKASRAKYIIVSLIIISFTTSCHFFETVTLQNLNHKNVNTNFSSDKGSNSIKKCQFKTEYTKFTNAWFFIDGAFYSWIPLFLLIINNLIILSEIYKARNIHNSMINTNHDENKTLGTSIVRTQLPRRMKESSSRNRETKQLTVMLLIVSFSFLITTSPFVILKLLIKLKG----YPNGDRKLAQLVLLDGIFEMLMYVNHAINFYLYCATGNKFRQQL 395
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000063133.1 (SMESG000063133.1)

HSP 1 Score: 186.037 bits (471), Expect = 2.545e-52
Identity = 136/358 (37.99%), Postives = 205/358 (57.26%), Query Frame = 1 
Query:  163 LKETLMKEIPGKLLYSCRLYHIFWTXXXXXXXXXXXXXNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTHVTKQFNKTNLIHSGE------CTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRS--ILMNNFNQACSKSIKKVST----------------------DTHHLSVTLIAVSFIFLITTTPLVVHKLLIH---FRDKDEVYHNGIIQFLDVIFEMLMYTNHSINFLLYCATGNRFRQHV 1137
             ++ +  +IPG+LL  C+++H+ W   PPI+ L+G++GN  A  VL    + + S+Y YLAV+   D+ IL+ GLLRR++   F    EN  ++ C++ QFIGV++SL+SVWLIVV+T+ER+I+I LPLR       K  + +I S  I +  ++SHFF TV  +  K      +    +      C  K ++  F ++WT LDA LYS +P  ++I +NV+I   I ++  +R   I MN+ +   +KSI    +                      +T  L++ L+ VS  FLITTTPLV+ K  I    +R+    Y    ++ +D IFEMLMYTNH+INFLLYCA+G +FR HV
Sbjct:  168 FRKDIQNKIPGRLLVQCQIFHLIWKAFPPILFLLGTLGNTTAFCVLVQRKLIRVSTYYYLAVLTCFDQSILIIGLLRRWVDKTFGFSSENNWLVCCQVFQFIGVATSLMSVWLIVVVTVERLIVIKLPLRVHSYVSIKKTKLIILSLFIITMTISSHFFETVTLNRRKNSKTLQMTTESKNHSFLICDFKTEYATFAKIWTFLDAFLYSCLPLVLMIVMNVVIGHGIYQADRSRGEMISMNSKDLRKTKSINSNKSTRTGNRIYGFTPRQAAPPLPNRETRQLTIMLLVVSCTFLITTTPLVLLKFFIKLNLYRNSSTSYITK-LELVDTIFEMLMYTNHAINFLLYCASGKKFRYHV 524
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000020718.1 (SMESG000020718.1)

HSP 1 Score: 175.252 bits (443), Expect = 3.083e-49
Identity = 129/339 (38.05%), Postives = 209/339 (61.65%), Query Frame = 1 
Query:  154 RLILKETLMKEIPGKLLYSCRLYHIFWTXXXXXXXXXXXXXNVLACIVLCCYLIDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGXXXXXXXXXXXXXMTIERVIIIYLPLRAKQITQKKIARAVISSTIICS--SLVASHFFFTVD-THVTKQFNKTNLIHSGECTAKPQFHYFYRVWTILDASLYSLIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHLSVTLIAVSFIFLITTTPLVVHKLL-----IHFRDKDEVYHNGII------QFLDVIFEMLMYTNHSINFLLYCATGNRFR 1128
            +LIL+E +    P + L  C L++   TIIP IIL  G+IGNV++ IVLC +     S++IYL+++ IVD+ +L+ GLL+R++ Y+  + IEN +  FC L+Q+ G S+S++SVWLIV +T+ER I+I  P +A  + QK  +RA++ S  + S   ++  HF FT+D T+ T+ F  T+  ++  C     +  F  VW ++DA +YS +PF  + +LNV+I+ ++ +++  R  +M   N+   KS+ + S  T  L++ L+ +S  FLITTTP+ + K+L        +   + +H   I      QF D I E+LM+ NH++NF LYCA+GN+FR
Sbjct:   58 KLILQENVYNANPSEELSLCVLHNYILTIIPAIILFFGTIGNVMSFIVLCNFAGKTVSTFIYLSLLAIVDQFVLIFGLLKRWVDYLLKRKIENRNKTFCILSQYFGTSASVLSVWLIVAVTLERAIVISFPFKAHNLIQK--SRAIVISISMASVIMILNCHFIFTLDLTNSTQLFESTD--NNFVCGFSESYRNFKTVWLLIDAIIYSFLPFIFIFALNVIILIKVCQARRTRQSMM-TLNRG-QKSVYR-SESTRQLTIMLLMISGTFLITTTPIFIVKMLYVVPKAFSKAIPKTFHAPGIRDAVASQFADSIAEILMHLNHALNFYLYCASGNKFR 389
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
K03H6.11.736e-826.52pep chromosome:WBcel235:IV:1523718:1526599:1 gene:... [more]
dmsr-71.867e-627.41DroMyoSuppressin Receptor related [Source:UniProt... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ghsrb1.834e-623.99growth hormone secretagogue receptor b [Source:ZFI... [more]
ghsra3.636e-625.68growth hormone secretagogue receptor a [Source:ZFI... [more]
ntsr16.675e-621.69neurotensin receptor 1 (high affinity) [Source:ZFI... [more]
ntsr16.675e-621.69neurotensin receptor 1 (high affinity) [Source:ZFI... [more]
mlnr7.743e-622.94motilin receptor [Source:ZFIN;Acc:ZDB-GENE-130530-... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H2KNW01.775e-4633.89G-protein coupled receptor OS=Clonorchis sinensis ... [more]
A0A1S8WXZ47.853e-4635.187 transmembrane receptor OS=Opisthorchis viverrini... [more]
A0A4S2KQC31.180e-4534.72G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A504YW109.570e-3734.08G-protein coupled receptor OS=Fasciola gigantica O... [more]
A0A183A9A81.885e-3633.61G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nmur34.754e-621.74neuromedin-U receptor 2-like [Source:NCBI gene;Acc... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nmur1a2.708e-621.45neuromedin U receptor 1a [Source:ZFIN;Acc:ZDB-GENE... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000029588.12.633e-621.40neuromedin-U receptor 1-like [Source:NCBI gene;Acc... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000061235.10.000e+099.28SMESG000061235.1[more]
SMESG000053893.11.291e-5640.28SMESG000053893.1[more]
SMESG000042142.17.064e-5541.10SMESG000042142.1[more]
SMESG000063133.12.545e-5237.99SMESG000063133.1[more]
SMESG000020718.13.083e-4938.05SMESG000020718.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035471 ID=SMED30035471|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1454bp
TGAGTTTTTAATTATTTGAAAATGTCGAGCACAAATATCTGTAACAATGT
TGAAAGTGATGGTGCAAATACGGCACTATTTCTGTTGAGTTTAAACGACA
GCCTTTTGTTACCTTATGCTTCGTCGACATCAAATTTATCAGTTCTGGAG
TTCAGACTAATCTTGAAAGAGACGCTCATGAAAGAAATTCCCGGTAAGCT
TTTGTATTCTTGTCGTTTGTATCACATATTCTGGACCATAATCCCACCAA
TAATATTATTGATCGGATCAATTGGGAATGTTTTAGCATGTATTGTACTT
TGTTGTTATCTCATTGACAAATGTTCTTCGTATATATATTTAGCTGTTAT
GGTAATCGTCGACGAAATGATACTTGTAACCGGACTTTTGAGACGATATA
TTGGATACATATTTATCAAACATATTGAGAACGAGTCGGTACTATTTTGT
CGACTTACTCAATTTATTGGCGTATCAAGCAGTTTGGTATCGGTATGGCT
AATCGTTGTAATGACCATTGAAAGGGTTATCATCATATATCTGCCTCTGA
GAGCAAAACAAATCACTCAGAAAAAAATTGCCAGAGCAGTAATAAGTTCA
ACAATTATATGCAGCTCACTCGTAGCCAGTCATTTTTTCTTTACTGTCGA
TACTCATGTGACGAAGCAGTTTAACAAAACAAATCTCATCCATTCCGGGG
AGTGTACTGCTAAACCTCAATTTCATTACTTCTATCGAGTTTGGACAATT
CTTGACGCATCTCTGTACAGTTTAATTCCATTTTGTGTTTTAATATCTCT
CAACGTTTTGATCGTTCGAGAAATTCTAAGATCAAAGAACAACCGGTCGA
TTCTCATGAATAACTTCAATCAAGCATGTAGTAAAAGTATTAAAAAGGTT
TCAACTGATACTCACCATTTATCAGTAACCTTAATTGCAGTATCGTTCAT
CTTTCTGATCACGACAACTCCTCTTGTTGTACATAAACTTCTGATCCATT
TCCGAGATAAAGACGAGGTCTATCATAATGGCATCATTCAATTTCTAGAT
GTTATTTTTGAAATGTTGATGTATACAAATCATTCCATCAATTTCCTTCT
ATATTGTGCAACTGGAAACCGCTTCCGACAACATGTCTTGTCGTTATTAT
TGTGTCGGACTCTGTCATTAAACAGACCTGCCGACAATACGAAATGTGCT
CGTTCGACAATCCAAATATCGGAGATGGATGCGATGTTACGCGGGAAACG
TTTCACAGTTTACCAGGAAGAATAACATTCTTGATAAAGTGGAAACTGTT
CTTCTTCTTGTTTTTATTAATTTATGACTGTAAATCAGAAATGTTTATTT
GATCCAAAAATTAAATAATATGAATATGTGGAAAAAATGAGAGCCATATA
AAATTAAAAAAACCATACCAATGAAGAATTATCAAAATATTTTAATGATG
TGTG
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protein sequence of SMED30035471-orf-1

>SMED30035471-orf-1 ID=SMED30035471-orf-1|Name=SMED30035471-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=418bp
MSSTNICNNVESDGANTALFLLSLNDSLLLPYASSTSNLSVLEFRLILKE
TLMKEIPGKLLYSCRLYHIFWTIIPPIILLIGSIGNVLACIVLCCYLIDK
CSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIG
VSSSLVSVWLIVVMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSL
VASHFFFTVDTHVTKQFNKTNLIHSGECTAKPQFHYFYRVWTILDASLYS
LIPFCVLISLNVLIVREILRSKNNRSILMNNFNQACSKSIKKVSTDTHHL
SVTLIAVSFIFLITTTPLVVHKLLIHFRDKDEVYHNGIIQFLDVIFEMLM
YTNHSINFLLYCATGNRFRQHVLSLLLCRTLSLNRPADNTKCARSTIQIS
EMDAMLRGKRFTVYQEE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 150..172
score: 21.65
coord: 70..94
score: 21.73
coord: 300..324
score: 20.81
coord: 343..369
score: 25.2
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 85..361
e-value: 4.7E-19
score: 68.7
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 63..383
e-value: 4.9E-45
score: 155.8
NoneNo IPR availablePANTHERPTHR46641:SF12CNMAMIDE RECEPTORcoord: 66..400
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 66..400
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 69..370
e-value: 6.39361E-33
score: 123.512
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 64..388
NoneNo IPR availableTMHMMTMhelixcoord: 189..208
NoneNo IPR availableTMHMMTMhelixcoord: 154..176
NoneNo IPR availableTMHMMTMhelixcoord: 339..361
NoneNo IPR availableTMHMMTMhelixcoord: 76..98
NoneNo IPR availableTMHMMTMhelixcoord: 243..265
NoneNo IPR availableTMHMMTMhelixcoord: 300..319
NoneNo IPR availableTMHMMTMhelixcoord: 105..127
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 85..361
score: 25.788