Na channel amiloride sensitive

Overview
NameNa channel amiloride sensitive
Smed IDSMED30035465
Length (bp)1762
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Na channel amiloride sensitive (SMED30035465) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Na channel amiloride sensitive (SMED30035465) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035465

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30035465SMESG000008099.1 SmedASXL_000547SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30035465SMESG000008099.1 SmedASXL_007628SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30035465SMESG000008099.1 SmedASXL_007629SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30035465SMESG000008099.1 dd_Smed_v4_61009_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30035465SMESG000008099.1 dd_Smed_v4_61009_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30035465SMESG000008099.1 dd_Smed_v6_61009_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Na channel amiloride sensitive vs. TrEMBL
Match: G4LY47 (Amiloride-sensitive sodium channel,putative OS=Schistosoma mansoni OX=6183 GN=Smp_175020 PE=3 SV=1)

HSP 1 Score: 317.005 bits (811), Expect = 5.262e-96
Identity = 189/492 (38.41%), Postives = 277/492 (56.30%), Query Frame = 1 
Query:    4 IFSQSSLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCHHQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKKIMKSSASDGMLPW------QHSRIKGIFLNITQNLSIISNNLERGLLKW-SSLTAKSPFYKLLHNITVNFKIIPYF--SGYQVTAYEQVPHVRSFLCTTFEFKTQSGYQPWVKLEILVNSNASLYHQSMNEISIITHEAGSLPFSAYDRHITETLNSGNKVQIFLSKSITMRLNTD-------RNPCNEY--------------------------TSTVNLFGRNYSYSHDVCGWKMCCTNCFKGCNCICPIDVLRQISNPCNET---CRQNSCARKG-NYTTCPSSCXXXXXXXXXXXXXXXXXX-----SHQLILIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIGFSCIYIFELIEAVILW-----HSGILTECINVPGGLRNDNIT 1311
            +FSQSSL+PL+KI+NAP++S++ILW +   +M IGL +CI+LVV+QY  HQT+FQLD+SG+   YD +P +T+C  ++    +F          L+A K +        LPW         R+K I +       II + L +G L W +S TA SP Y++L N +V F+I P +    Y++T  EQVP++R+FLCTTFE ++ +  + W  LEI +N  +++ + +     IITHE G LPFSAYDRHI   L     V ++ SKS+T RLNTD       +N    +                          T+TV+LFG  + YS + CGW  CCT     CNC C +D+L  I+    +T   C +  C  +   Y TCP  CI  K+ KH++      ++     + +L L++S++V +A EE+I+++ KLFSE+GGLCSLFIGFSCI++FEL+EA+IL      H     +C+  P    N +IT
Sbjct:    7 VFSQSSLVPLAKIANAPSRSLRILWAIVTAIMFIGLCVCISLVVMQYLKHQTVFQLDYSGRHTVYDQVPGITICPQSQESKRLF----------LDAMKRLNIITQPIPLPWFSKTVMDQLRMKVIEIQPDLKFGIIVHRLPKGELLWNTSKTAISPIYRMLLNFSVEFQIQPSYLADSYRLTVMEQVPNIRNFLCTTFELRSNNVEKSWSYLEIRINQESTIENSNRVTFIIITHERGELPFSAYDRHIHTNLPPDTSVSLYFSKSVTTRLNTDYLDTIRMKNKQQTFVSDTTNYLQFALKRLKQKTKEQYLSQTNTVSLFGTEFLYSREACGWVECCTKVAYHCNCTCTLDLL-SINRTALQTHFLCERTQCQDEQIPYETCPLPCIQTKFIKHSEVRLPGYEDGLPILTSRLKLVRSESVQVAMEEEIFSLAKLFSEVGGLCSLFIGFSCIFLFELLEALILMYYPNIHKMNDQKCLTTPFIQANRHIT 487
[Close]
BLAST of Na channel amiloride sensitive vs. TrEMBL
Match: A0A0R3VSP1 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS194 PE=3 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 2.447e-83
Identity = 181/511 (35.42%), Postives = 261/511 (51.08%), Query Frame = 1 
Query:    1 KIFSQSSLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCHHQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKKIMKSSASDGMLPWQHSRIKGIFLN-ITQNLSI-----ISNNLERGLLKWSS-LTAKSPFYKLLHNITVNFKIIP--YFSGYQVTAYEQVPHVRSFLCTTFEFKTQSGYQPWVKLEILVNSN---ASLYHQSMNEI-------SIITHEAGSLPFSA------------------YDRHIT--------------------ETLNSGNKVQIFLS--------------------------KSITMRLNTDRNPCNEYTS-TVNLFGRNYSYSHDVCGWKMCCTNCFKGCNCICPIDVLRQIS--NPCNETCRQNSCARKGN-YTTCPSSCXXXXXXXX-----XXXXXXXXXXSHQLILIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIGFSCIYIFELIEAVILWHSG 1257
            ++FSQSSL+P++KI++APT+  +I+W +  T M IGL  CITLV+IQYC HQT+FQLD+SG+   YD IP +T+C + + +  +F N   +  G  EA K     A  G +PW +S +    L  IT N ++     I + L  G L W + + AKSP Y++LHN +V F+I P    + YQ+   EQVP+VR F+CTTFE  T+     W  +EI +  +   A +   + + +        II HE G LPFSA                  Y + +T                    +TL    K   FL                           ++  MR N +R P  + ++  V+LF   + YS + CGWK+CC      C C C +D L   +  N  N+ C +  C+     Y  CP  C++ K+ K           K+  N+ +L LI+S+NV +A EE+IY++ KLFSEIGGLCSLFIGFSCI+IFE +EA++L   G
Sbjct:    6 EVFSQSSLVPIAKIASAPTRLQRIIWVIISTAMFIGLCSCITLVIIQYCMHQTVFQLDYSGRHTVYDQIPGITICPDPQEIHRLF-NYARKVRGWPEANK----KAEQGTVPWLNSAVNKELLKLITANRALDPPGAIGHYLPSGSLYWDTQVVAKSPIYRVLHNFSVTFQIKPANVLNNYQLFVLEQVPNVRQFMCTTFELTTRRPEHGWSYVEITIQQDMHMAGIATDTADSVKPRGTTFGIIAHERGELPFSAYDRNIYTTLAPENEVSIYYSKSVTRRANTARSPCHDGADAAWLAQTLEETGKNGTFLDFLDRRRLNIKKKRAWPTDNVPYLPDHQATDMRTNEERQPATQKSAKAVSLFNTEFLYSREACGWKVCCMKVALECGCTCTMDWLLSTATENCPNDRCERTQCSDSPVLYNQCPMPCVLSKFVKQNLLIIEPPDSKLPANTSKLKLIRSENVQVAAEEEIYSLAKLFSEIGGLCSLFIGFSCIFIFEFLEAMLLIKRG 511
[Close]
BLAST of Na channel amiloride sensitive vs. TrEMBL
Match: A0A4S2L784 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009542 PE=3 SV=1)

HSP 1 Score: 236.884 bits (603), Expect = 2.072e-65
Identity = 126/298 (42.28%), Postives = 176/298 (59.06%), Query Frame = 1 
Query:    4 IFSQSSLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCHHQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKKIMKSSASDGMLPWQH----SRIKGIFLNITQNLS--IISNNLERGLLKW-SSLTAKSPFYKLLHNITVNFKIIPYFSG--YQVTAYEQVPHVRSFLCTTFEFKTQSGYQPWVKLEILVNSNASLYHQSMNEISIITHEAGSLPFSAYDRHITETLNSGNKVQIFLSKSITMRLNTDRNPCNEYTSTVNLFGRNYSYSHD 870
            +FSQSSL+PL+KI+NAPT+ +++LW +F   M + L +CITLVVIQYC HQT+FQLD+SG+ + YD IP +T+C        +F              + +    +D  LPWQ      + +   L  + NLS  +I++ L  G L W +S TA SP Y+LLHN TV F + P  S   Y++T  EQVP+ RSFLCTTFE ++ S    W  LE+ ++ +AS+        +IITHE G LPFSAYDRHI   +N  + + ++ SKS+T RL T RNPC+E     ++       S D
Sbjct:    6 VFSQSSLVPLAKIANAPTRPLRLLWAVFTATMFMVLCVCITLVVIQYCKHQTVFQLDYSGRYMVYDQIPGITICPQAAEAKKLF-------------SRFVAMPGTDTRLPWQDKILIEQFRQRVLASSPNLSFDVITHRLPVGELVWNTSTTAISPIYQLLHNFTVRFNVQPPKSARSYRLTVMEQVPNARSFLCTTFELRSPSPKDRWSYLEVEIDQSASI--DDPISFTIITHERGELPFSAYDRHIHTIINPKSIISLYFSKSVTSRLQTARNPCHEGPDAKSMAAEYAKSSED 288

HSP 2 Score: 114.775 bits (286), Expect = 7.536e-23
Identity = 75/201 (37.31%), Postives = 112/201 (55.72%), Query Frame = 1 
Query:  715 DRHITETLNSGNKVQIFLSK--SITMRLNTDRNPCN-----------EYTSTVNLFGRNYSYSHDVCGWKMCCTNCFKGCNCICPIDVLRQISNPC--NETCRQNSCARKG-NYTTCPSSCXXXXXXXXXXXXXXXXXXSH-----QLILIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIGFSCIYIFELIEAVILWHS 1254
            DR +T   +  +K+++ L++  S++ R     NP N           E +  V LFG  + Y  + CGW  C     + C C C + VL      C     C +  C  +  +Y  CP  C++ K+ KHN+      + +      +L LI+S++VH+ATEE+I+++ KLFSE+GGLCSLFIGFSCI++FEL+EAVIL HS
Sbjct:  312 DRQVTWYEDFLDKLRLNLTQTNSLSTR-EAQSNPQNPSKVDRASKFQEKSKAVLLFGTAFLYGRESCGWAECWRQVLRECGCNCTLHVLGYNEAACRGGPACEKKHCVDQQISYKICPLPCVMTKFIKHNEVTVSGAEANISVGVCKLKLIRSESVHVATEEEIFSLAKLFSEVGGLCSLFIGFSCIFLFELLEAVILMHS 511
[Close]
BLAST of Na channel amiloride sensitive vs. TrEMBL
Match: A0A3Q0KTE1 (Amiloride-sensitive sodium channel,putative OS=Schistosoma mansoni OX=6183 PE=3 SV=1)

HSP 1 Score: 230.72 bits (587), Expect = 3.689e-62
Identity = 119/280 (42.50%), Postives = 172/280 (61.43%), Query Frame = 1 
Query:    4 IFSQSSLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCHHQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKKIMKSSASDGMLPW------QHSRIKGIFLNITQNLSIISNNLERGLLKW-SSLTAKSPFYKLLHNITVNFKIIPYF--SGYQVTAYEQVPHVRSFLCTTFEFKTQSGYQPWVKLEILVNSNASLYHQSMNEISIITHEAGSLPFSAYDRHITETLNSGNKVQIFLSKSITMRLNTDRNPCN 816
            +FSQSSL+PL+KI+NAP++S++ILW +   +M IGL +CI+LVV+QY  HQT+FQLD+SG+   YD +P +T+C  ++    +F          L+A K +        LPW         R+K I +       II + L +G L W +S TA SP Y++L N +V F+I P +    Y++T  EQVP++R+FLCTTFE ++ +  + W  LEI +N  +++ + +     IITHE G LPFSAYDRHI   L     V ++ SKS+T RLNT RNPC+
Sbjct:    7 VFSQSSLVPLAKIANAPSRSLRILWAIVTAIMFIGLCVCISLVVMQYLKHQTVFQLDYSGRHTVYDQVPGITICPQSQESKRLF----------LDAMKRLNIITQPIPLPWFSKTVMDQLRMKVIEIQPDLKFGIIVHRLPKGELLWNTSKTAISPIYRMLLNFSVEFQIQPSYLADSYRLTVMEQVPNIRNFLCTTFELRSNNVEKSWSYLEIRINQESTIENSNRVTFIIITHERGELPFSAYDRHIHTNLPPDTSVSLYFSKSVTTRLNTGRNPCH 276

HSP 2 Score: 117.087 bits (292), Expect = 1.428e-23
Identity = 74/190 (38.95%), Postives = 112/190 (58.95%), Query Frame = 1 
Query:  730 ETLNSGNKVQIFLSKS---ITMRLNTDRNPCNEY----TSTVNLFGRNYSYSHDVCGWKMCCTNCFKGCNCICPIDVLRQISNPCNET---CRQNSCARKG-NYTTCPSSCXXXXXXXXXXXXXXXXXX-----SHQLILIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIGFSCIYIFELIEAVILWH 1251
            +T+   NK Q F+S +   +   L   +    E     T+TV+LFG  + YS + CGW  CCT     CNC C +D+L  I+    +T   C +  C  +   Y TCP  CI  K+ KH++      ++     + +L L++S++V +A EE+I+++ KLFSE+GGLCSLFIGFSCI++FEL+EA+IL +
Sbjct:  363 DTIRMKNKQQTFVSDTTNYLQFALKRLKQKTKEQYLSQTNTVSLFGTEFLYSREACGWVECCTKVAYHCNCTCTLDLL-SINRTALQTHFLCERTQCQDEQIPYETCPLPCIQTKFIKHSEVRLPGYEDGLPILTSRLKLVRSESVQVAMEEEIFSLAKLFSEVGGLCSLFIGFSCIFLFELLEALILMY 551
[Close]
BLAST of Na channel amiloride sensitive vs. TrEMBL
Match: A0A504Y611 (Na channel amiloride sensitive OS=Fasciola gigantica OX=46835 GN=FGIG_02532 PE=3 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 8.844e-62
Identity = 119/282 (42.20%), Postives = 174/282 (61.70%), Query Frame = 1 
Query:    4 IFSQSSLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCHHQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKKIMKSSASDGMLPWQHSRIKGIFLNITQ----NLS--IISNNLERGLLKWSSL-TAKSPFYKLLHNITVNFKIIPYFSG--YQVTAYEQVPHVRSFLCTTFEFKTQS-GYQPWVKLEILVNSNASLYHQSM-NEISIITHEAGSLPFSAYDRHITETLNSGNKVQIFLSKSITMRLNTDRNPCN 816
            +FSQSSL+PL+KI+NAP++S+++LW +F   M +GL  CITLVVIQYC HQT+FQLD+SG+ V YD IP +TLC  T+    +F   +  +V    +             PW + +    F  I +    N+S  ++++ +  G L+W +  +A SP Y +LHN +V F + P  +G  +++T  EQVP+ + FLCTTFE ++Q+     W  LE+ +N  A+     M +   +ITHE G LPFSAYDRHI  ++  G+ V ++ SKSIT RLNT RNPC+
Sbjct:    7 VFSQSSLVPLAKIANAPSRSLRLLWAVFTATMFVGLCACITLVVIQYCRHQTVFQLDYSGRNVIYDQIPGLTLCPQTQEAKRLFHLAVNMEVNKHPS-------------PWLNKQALDKFRQIAKQRKPNMSFEVLTHRMPPGQLQWDTQKSALSPLYGMLHNFSVRFVMQPAQTGDSHRLTVMEQVPNPKHFLCTTFELRSQNPSVSRWSYLEMQLNQTATPPGSDMFSSFILITHERGELPFSAYDRHIQTSMTPGSDVSVYFSKSITARLNTARNPCH 275

HSP 2 Score: 114.775 bits (286), Expect = 6.199e-23
Identity = 61/150 (40.67%), Postives = 90/150 (60.00%), Query Frame = 1 
Query:  826 STVNLFGRNYSYSHDVCGWKMCCTNCFKGCNCICPIDVLRQISNPCN--ETCRQNSCARKG-NYTTCPSSCXXXX-----XXXXXXXXXXXXXXSHQLILIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIGFSCIYIFELIEAVILWH 1251
            STV LFG  + YS + CGW  C     + CNC   +++     + C   E C +  C  K  +Y TCP +C++ K           +   +   + Q+ L++S+ V +ATEE+I+++ KLFSE+GGLCSLFIGFSCI++FEL+EAVIL +
Sbjct:  358 STVRLFGTTFFYSRESCGWAECSRKVRQVCNCSSVVELWSYKMHGCEAVEACERAECQVKQISYKTCPLACVVAKFVKHNTITETGSEVNLAPGAIQIKLVRSEAVQVATEEEIFSLAKLFSEVGGLCSLFIGFSCIFLFELVEAVILMY 507
[Close]
BLAST of Na channel amiloride sensitive vs. Planmine SMEST
Match: SMESG000008099.1 (SMESG000008099.1)

HSP 1 Score: 1036.56 bits (2679), Expect = 0.000e+0
Identity = 522/524 (99.62%), Postives = 523/524 (99.81%), Query Frame = 1 
Query:    1 KIFSQSSLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCHHQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKKIMKSSASDGMLPWQHSRIKGIFLNITQNLSIISNNLERGLLKWSSLTAKSPFYKLLHNITVNFKIIPYFSGYQVTAYEQVPHVRSFLCTTFEFKTQSGYQPWVKLEILVNSNASLYHQSMNEISIITHEAGSLPFSAYDRHITETLNSGNKVQIFLSKSITMRLNTDRNPCNEYTSTVNLFGRNYSYSHDVCGWKMCCTNCFKGCNCICPIDVLRQISNPCNETCRQNSCARKGNYTTCPSSCXXXXXXXXXXXXXXXXXXSHQLILIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIGFSCIYIFELIEAVILWHSGILTECINVPGGLRNDNITRYSFRGKDVDKSSEHCALFSQTSSNKDIPFSFSISLEEQTLSPVSRFNFDFDGNKTPAVFLIKTQNEFEKGRFINAVKEGRARLKVE 1572
            KIFSQSSLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCHHQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKKIMKSSASDGMLPWQHSRIKGIFLNIT NLSIISNNLERGLLKWSSLTAKSPFYKLLHNITVNFKIIPYFSGYQVTAYEQVPHVRSFLCTTFEFKTQSGYQPWVKLEILVNSNASLYHQSMNEISIITHEAGSLPFSAYDRHITETLNSGNKVQIFLSKSITMRLNTDRNPCNEYTST+NLFGRNYSYSHDVCGWKMCCTNCFKGCNCICPIDVLRQISNPCNETCRQNSCARKGNYTTCPSSCIIIKYNKHNKNIEKINKNSHQLILIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIGFSCIYIFELIEAVILWHSGILTECINVPGGLRNDNITRYSFRGKDVDKSSEHCALFSQTSSNKDIPFSFSISLEEQTLSPVSRFNFDFDGNKTPAVFLIKTQNEFEKGRFINAVKEGRARLKVE
Sbjct:    3 KIFSQSSLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCHHQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKKIMKSSASDGMLPWQHSRIKGIFLNITHNLSIISNNLERGLLKWSSLTAKSPFYKLLHNITVNFKIIPYFSGYQVTAYEQVPHVRSFLCTTFEFKTQSGYQPWVKLEILVNSNASLYHQSMNEISIITHEAGSLPFSAYDRHITETLNSGNKVQIFLSKSITMRLNTDRNPCNEYTSTINLFGRNYSYSHDVCGWKMCCTNCFKGCNCICPIDVLRQISNPCNETCRQNSCARKGNYTTCPSSCIIIKYNKHNKNIEKINKNSHQLILIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIGFSCIYIFELIEAVILWHSGILTECINVPGGLRNDNITRYSFRGKDVDKSSEHCALFSQTSSNKDIPFSFSISLEEQTLSPVSRFNFDFDGNKTPAVFLIKTQNEFEKGRFINAVKEGRARLKVE 526
[Close]
BLAST of Na channel amiloride sensitive vs. Planmine SMEST
Match: SMESG000002742.1 (SMESG000002742.1)

HSP 1 Score: 239.58 bits (610), Expect = 9.456e-74
Identity = 134/278 (48.20%), Postives = 173/278 (62.23%), Query Frame = 1 
Query:  538 VPHVRSFLCTTFEFKTQSGYQPWVKLEILVNS------NASLYHQSMNEISIITHEAGSLPFSAYDRHITETLNSGNKVQIFLSKSITMRLNTDRNPCNEYTSTVNLFGRNYSYSHDVCGWKMCCTNCFKGCNCICPIDVLRQISNPCNETCRQNSCARKGNYTTCPSSCXXXXXXXXXXXXXXXXXXSHQLILIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIGFSCIYIFELIEAVILWHSGILTECINVPGGLRNDNITRYSFRGKDVDKSSE 1353
            VP    F+CTTFEF+       W  + ++V +      N +       +++ I HE GSLP+SAYD+HITET+  G+++ +FLSKS+T RLNT RNPC     T +LFG +Y Y HD CGWK CC    K CNC C IDVLR    PC   C +  C  + +Y  CPSSC + KYNKHN   +K  K+ ++L L+KSDNVHIATEE++YT+TKL SEIGGLCSLFIGFSCI++FE+IEA I+W S              ND+ T Y  +  DV  S +
Sbjct:    8 VPRTWGFICTTFEFENSDPDYSWSGINVVVENELDTVINQTPEKLKRYQVNFIVHEKGSLPYSAYDKHITETIMGGSELTVFLSKSVTRRLNTKRNPCLA-KGTTSLFGNDYEYGHDACGWKECCIKTLKFCNCTCDIDVLRHSLRPCPIGCVKKVCLSQISYEVCPSSCTVAKYNKHNILEQKCEKHMNKLNLVKSDNVHIATEEELYTLTKLCSEIGGLCSLFIGFSCIFLFEIIEAFIIWRSSAYI----------NDSNTLYLSKSLDVSNSPQ 274
[Close]
BLAST of Na channel amiloride sensitive vs. Planmine SMEST
Match: SMESG000000681.1 (SMESG000000681.1)

HSP 1 Score: 179.489 bits (454), Expect = 2.795e-50
Identity = 127/409 (31.05%), Postives = 194/409 (47.43%), Query Frame = 1 
Query:   19 SLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCHHQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKKIMKSSASDGMLPWQHSRIKGIFLNITQNLSIISNNLERGLLKWSSLTAKSPFYKLLHNITVNFKIIPYFSGYQVTAYEQVPHVRSFLCTTFEFKTQSGYQPWVKLEILVNSNASLYHQSMNEISIITHEAGSLPFSAYDRHITETLNSGNKVQIFLSKSITMRLNTDRNPCNEYTSTVNLFGRNYSYSHDVCGWKMCCTNCFKGCNCICPIDVLRQISNPCNETCRQNSCARKGNYTTCPSSCXXXXXXXXXXXXXXXXXXSHQLI-LIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIGFSCIYIFELIEAVI 1242
            SLIPL+KI NA ++  +ILWT+F   M++GLI  I L V QY    T+F++D+S      D +PA+T+CHN                                     H   K ++ ++    +II N        WS+  A S F   L         +P                ++ +CT FE   +   + W  L+I +           +   ++ H   S PF+ Y++++ + ++ G  +  F +KS+   L+T+RNPC      +N+ GRN+SY   VC W   C N    CNC C +DVL  I + C++ C Q       N + CP +C  +KY+  N     +++ +   I +I S+ V +ATEE IYT+ K+FSEIGGL SL  G SCI IFEL+E ++
Sbjct:    6 SLIPLTKILNAHSQLSRILWTIFSVAMVVGLIGSIILTVHQYLDQSTVFRMDYSTANYLND-LPAITVCHN------------------------------------NHRINKEVYHSVIGESTIIYNITPHSTANWSTENALSLFIGNLKTT------LP----------------KNQICTIFELYLEKS-EAWNSLKITMEQPDP---NPTDFFEVVIHGKESFPFNNYEKYLYKKVDPGKCIYTFTAKSVANHLDTNRNPCENIK--INVMGRNFSYDQTVCQWMHVCVNLHNYCNCSCELDVLGVIHSRCSDKCLQKFSRALVNTSICPQACHKMKYSDLNVVTRDLSQYNKSEIEIIMSNLVSMATEEPIYTLAKMFSEIGGLSSLLFGVSCIIIFELLEKLL 349
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BLAST of Na channel amiloride sensitive vs. Planmine SMEST
Match: SMESG000002761.1 (SMESG000002761.1)

HSP 1 Score: 147.132 bits (370), Expect = 8.944e-41
Identity = 72/166 (43.37%), Postives = 104/166 (62.65%), Query Frame = 1 
Query:    4 IFSQSSLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCHHQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKKIMKSSASDGMLPWQHSRIKGIFLNITQNLSIISNNLERGLLKWSSLTAKSPFYKLLHNITVNFKIIP 501
            +F  SSLIPLSKI+NAP+KSI+ILW +F   MLIGL++CI  VV QYC  QTIFQLD+SGK  + D  P++++C     + +MF   ++  +   E + +MK +      PW +S +   F  IT  L++I N +    L+W++ +A S  YK++HN TV F I P
Sbjct:   11 LFGSSSLIPLSKIANAPSKSIRILWIIFSITMLIGLLVCIGFVVNQYCLQQTIFQLDYSGKMTSLDNCPSISICFLNDEVQNMFMQAMKTVLNRQEYEDLMKKNKDSRKPPWLYSGVHSFFRKITDRLTVIQNKITNSSLQWTTHSAISKLYKVMHNFTVKFSISP 176
The following BLAST results are available for this feature:
BLAST of Na channel amiloride sensitive vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G4LY475.262e-9638.41Amiloride-sensitive sodium channel,putative OS=Sch... [more]
A0A0R3VSP12.447e-8335.42Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
A0A4S2L7842.072e-6542.28Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A3Q0KTE13.689e-6242.50Amiloride-sensitive sodium channel,putative OS=Sch... [more]
A0A504Y6118.844e-6242.20Na channel amiloride sensitive OS=Fasciola giganti... [more]
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BLAST of Na channel amiloride sensitive vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Na channel amiloride sensitive vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000008099.10.000e+099.62SMESG000008099.1[more]
SMESG000002742.19.456e-7448.20SMESG000002742.1[more]
SMESG000000681.12.795e-5031.05SMESG000000681.1[more]
SMESG000002761.18.944e-4143.37SMESG000002761.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035465 ID=SMED30035465|Name=Na channel amiloride sensitive|organism=Schmidtea mediterranea sexual|type=transcript|length=1762bp
AAAATATTCTCTCAAAGCTCTTTAATACCTCTTTCTAAAATCTCTAATGC
TCCAACAAAATCAATAAAAATATTATGGACTTTGTTTTTTACTGTAATGC
TCATTGGGTTGATAATATGCATCACTTTAGTTGTGATTCAATATTGTCAT
CATCAAACCATTTTCCAGCTTGATTTTTCCGGCAAACAAGTTGCCTATGA
TGCAATTCCTGCCGTTACTCTCTGTCATAATACCAAGAATATGGTTTCAA
TGTTTAGGAATCTTTTGGAAAGGCAGGTAGGTGTCCTTGAAGCAAAAAAA
ATTATGAAATCAAGTGCAAGTGACGGAATGCTTCCATGGCAGCATTCAAG
AATCAAGGGAATCTTTTTGAATATAACACAAAATCTTTCGATTATTTCAA
ATAATTTAGAGAGAGGACTTTTAAAATGGTCGAGTTTAACAGCTAAATCT
CCATTTTATAAACTTCTTCACAATATCACTGTTAACTTTAAAATTATCCC
ATACTTTTCAGGCTACCAAGTAACTGCTTATGAACAGGTACCCCACGTGA
GGAGTTTCCTTTGTACTACATTTGAATTTAAAACCCAAAGCGGTTATCAA
CCGTGGGTAAAATTGGAGATTCTTGTCAATAGTAATGCCAGTCTCTATCA
TCAATCTATGAATGAAATTAGTATAATAACTCATGAAGCTGGATCCCTCC
CATTTTCTGCATATGACAGACATATCACAGAGACATTAAATTCCGGAAAC
AAAGTTCAGATTTTTCTGTCAAAGTCCATTACAATGCGCCTCAATACTGA
TCGGAATCCGTGCAATGAATACACAAGTACTGTTAACTTATTTGGTAGGA
ACTATTCTTATAGTCATGATGTATGTGGCTGGAAAATGTGTTGCACCAAT
TGCTTCAAAGGTTGTAACTGTATATGCCCCATTGATGTACTTCGACAGAT
TTCAAATCCATGTAATGAGACATGTCGGCAAAATAGCTGTGCACGGAAAG
GTAATTATACAACTTGCCCATCTTCTTGTATAATAATAAAGTACAATAAG
CATAACAAAAACATTGAAAAGATTAATAAAAATAGCCATCAGTTAATTCT
GATCAAAAGTGACAATGTTCACATTGCTACGGAAGAAAAGATTTATACAA
TCACAAAATTATTCAGTGAAATCGGTGGCCTGTGCTCCCTATTCATTGGA
TTCAGTTGCATTTACATTTTCGAGTTAATAGAAGCCGTCATCCTATGGCA
CAGTGGCATTCTGACGGAATGCATCAATGTACCAGGAGGTTTGAGAAATG
ACAATATTACTCGATATTCTTTTCGTGGCAAAGACGTCGACAAATCAAGT
GAACACTGTGCATTATTTTCTCAGACAAGTTCCAATAAAGATATTCCTTT
CTCCTTTTCCATTAGCTTAGAAGAACAAACTCTTAGCCCAGTTTCACGTT
TTAATTTCGATTTTGATGGAAATAAAACCCCTGCGGTATTCTTAATCAAG
ACTCAAAATGAGTTTGAAAAAGGCAGATTTATCAATGCAGTTAAAGAAGG
CAGGGCAAGATTGAAGGTTGAGTAATCAAGAGTTAGAACATACAATAACT
GAAAAAACCTAATTTTGAAATATTTGCAATTTGAATCACTGATTTTAATG
GTTTGTTAATTAGGAAAGACTTTTATTTATTTCACCATTGATTTTTTTCC
AATTCCTATTGATACGCCTCCAGGCGACGTCACAGTTGTTTCATGTAATT
TAGAGGTTGAAG
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protein sequence of SMED30035465-orf-1

>SMED30035465-orf-1 ID=SMED30035465-orf-1|Name=SMED30035465-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=525bp
KIFSQSSLIPLSKISNAPTKSIKILWTLFFTVMLIGLIICITLVVIQYCH
HQTIFQLDFSGKQVAYDAIPAVTLCHNTKNMVSMFRNLLERQVGVLEAKK
IMKSSASDGMLPWQHSRIKGIFLNITQNLSIISNNLERGLLKWSSLTAKS
PFYKLLHNITVNFKIIPYFSGYQVTAYEQVPHVRSFLCTTFEFKTQSGYQ
PWVKLEILVNSNASLYHQSMNEISIITHEAGSLPFSAYDRHITETLNSGN
KVQIFLSKSITMRLNTDRNPCNEYTSTVNLFGRNYSYSHDVCGWKMCCTN
CFKGCNCICPIDVLRQISNPCNETCRQNSCARKGNYTTCPSSCIIIKYNK
HNKNIEKINKNSHQLILIKSDNVHIATEEKIYTITKLFSEIGGLCSLFIG
FSCIYIFELIEAVILWHSGILTECINVPGGLRNDNITRYSFRGKDVDKSS
EHCALFSQTSSNKDIPFSFSISLEEQTLSPVSRFNFDFDGNKTPAVFLIK
TQNEFEKGRFINAVKEGRARLKVE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR001873ENaC
Vocabulary: biological process
TermDefinition
GO:0006814sodium ion transport
GO:0006810transport
GO:0006811ion transport
GO:0035725sodium ion transmembrane transport
Vocabulary: molecular function
TermDefinition
GO:0005272sodium channel activity
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001873Epithelial sodium channelPRINTSPR01078AMINACHANNELcoord: 378..392
score: 32.27
coord: 65..81
score: 28.71
coord: 321..341
score: 22.86
coord: 392..408
score: 37.88
IPR001873Epithelial sodium channelPFAMPF00858ASCcoord: 5..411
e-value: 6.9E-27
score: 94.5
IPR001873Epithelial sodium channelPANTHERPTHR11690AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATEDcoord: 23..415
NoneNo IPR availableGENE3DG3DSA:2.60.470.10coord: 53..380
e-value: 1.7E-13
score: 52.3
NoneNo IPR availableGENE3DG3DSA:1.10.3590.10coord: 78..222
e-value: 1.7E-13
score: 52.3
NoneNo IPR availableGENE3DG3DSA:1.10.287.770coord: 23..410
e-value: 1.7E-13
score: 52.3
NoneNo IPR availableGENE3DG3DSA:1.10.287.820coord: 263..343
e-value: 1.7E-13
score: 52.3
NoneNo IPR availableTMHMMTMhelixcoord: 24..46