SMED30035435
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035435 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of SMED30035435 vs. TrEMBL
Match: A0A5J4NUP0 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0004351 PE=4 SV=1) HSP 1 Score: 64.3142 bits (155), Expect = 6.880e-7 Identity = 60/253 (23.72%), Postives = 104/253 (41.11%), Query Frame = 3 Query: 387 LPNAKYKLTIDIVKTIKGSLGEWKSPILMAGEFSTFRDSNNF--CQFQSQANAEQVYFLEAVSIKNGEFKVLFNPEIYNDLQLDNIYTSICPGCGKTPSVTVKDIS-KSVDFLVAECMSDGYPAPVTKWKIQKKYAATI-----IETIKGGVRLIQPINNSTNEVQV------CEAVNVIGSASYSIVVKPVIFKTQVVK-------IKESQIIPCSTNDEKFCNGKGKCYEDKSEKNSKFCRCNKGYMGPNC 1082 L + K+ T+ + K + E + I + F+ DS++ C N++ ++FL + + V +NP Y + NI C C + D+ K L C + G P+P W K + IE IK ++ + +N +++ C A N +G AS +F Q+ + + +++++PCS D +C G+CY KS++N CRC Y G C Sbjct: 135 LADQKFNATVLVTNVYKSFVHE-QDQITVGSFFTPTVDSHDGPGCLDAFYRNSKYIFFLRRNMNADNFYDVTYNPHPYTEELDQNIQAKQCVNCFAPKIQPLNDLMLKPGSKLAVTCSASGKPSPELVWVKNGKSLNLVDKSISIEMIKVSEDNVESVLEISNLIRIDSGEYKCLATNPLGKASE-------LFNLQITQPEKSGQLLMDTELVPCSDEDRDYCLNGGQCYMLKSDRNVLQCRCPHQYFGDRC 379
BLAST of SMED30035435 vs. TrEMBL
Match: A0A5K4EN02 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 61.2326 bits (147), Expect = 6.061e-6 Identity = 57/217 (26.27%), Postives = 79/217 (36.41%), Query Frame = 3 Query: 636 IYTSICPGCGKTPSVTVKDISKSV----DFLVAECMSDGYPAPVTKW-----KIQKKYAATIIETIK---GGVRLIQPINN----STNEVQVCEAVNVIGSASYSIVVKPVIFKTQVVKIKESQIIPCSTNDEKFCNGKGKCYEDKSEKNSKFCRCNKGYMGPNCASVSRSVSNNLVERLKNMDKIFCNNQGISLKNYTTQTIYCECYLNFFGNHCE 1238 IY C C K T++ I V L C++ G P P W I +ET + G V I INN T E C A N +G A +K K+ +++PCS D+ +C G+CY K+E+ S CRC Y G C ++ T +Y CY +FG+ CE Sbjct: 193 IYGLYCHNCVKP---TIESIPNQVLEYGHSLTTSCLATGTPTPTIMWIRDGKSINLADKGLTVETFERSPGYVESILEINNLILIDTGEYW-CYAENALGLAKEKFTLKVQPNNRTEEKLMVDELVPCSDEDKDYCLNGGQCYTYKNERVSFQCRCLDHYFGDRC-------------------------------HFHTDGLYGNCYTPYFGSKCE 374
BLAST of SMED30035435 vs. TrEMBL
Match: A0A4S2L7F9 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009321 PE=4 SV=1) HSP 1 Score: 60.077 bits (144), Expect = 9.649e-6 Identity = 49/198 (24.75%), Postives = 89/198 (44.95%), Query Frame = 3 Query: 534 NAEQVYFLEAVSIKNGEFKVLFNPEIYNDLQLDNIYTSICPGCGKTPSVTVKDIS-KSVDFLVAECMSDGYPAPVTKWKIQKKYAATI-----IETIKGGVRLIQPINNSTNEVQV------CEAVNVIGSASYSI---VVKPVIFKTQVVKIKESQIIPCSTNDEKFCNGKGKCYEDKSEKNSKFCRCNKGYMGPNC 1082 N++ ++FL+ V G + + NP+++ + +IY C C + ++ K + L C++ G P P W K + +E IK + I ++ +++ C A N +G AS S V +P K + V + ++++I C +C G+CY KS++N CRC++ Y G C Sbjct: 168 NSKYMFFLKKVGTPAGYYAMTHNPQLFTEKLAYSIYARRCSSCYAPKIEPISNLEMKPGEQLTITCVAMGKPLPEVTWIKNGKSLNLVDKSVRVEEIKISDEQTESILEISSLIRIDSGEYKCLAHNSLGEASVSFSLQVSQPD--KPEQVSM-DTELIECPEEHRDYCLNGGQCYMLKSDRNVLQCRCSQQYFGDRC 362
BLAST of SMED30035435 vs. Planmine SMEST
Match: SMESG000040472.1 (SMESG000040472.1) HSP 1 Score: 878.626 bits (2269), Expect = 0.000e+0 Identity = 472/473 (99.79%), Postives = 472/473 (99.79%), Query Frame = 3 Query: 225 MNIITLFLYQCCFAFYQIKAGNLCDKLNDFGDNIQTKAILAPXXXXXXXXXXXDLPNAKYKLTIDIVKTIKGSLGEWKSPILMAGEFSTFRDSNNFCQFQSQANAEQVYFLEAVSIKNGEFKVLFNPEIYNDLQLDNIYTSICPGCGKTPSVTVKDISKSVDFLVAECMSDGYPAPVTKWKIQKKYAATIIETIKGGVRLIQPINNSTNEVQVCEAVNVIGSASYSIVVKPVIFKTQVVKIKESQIIPCSTNDEKFCNGKGKCYEDKSEKNSKFCRCNKGYMGPNCASVSRSVSNNLVERLKNMDKIFCNNQGISLKNYTTQTIYCECYLNFFGNHCENRYYMKAPVNEGLKEFITEQNSKLAVKYXXXXXXXXXXXXXXXXXXXXXXWRFYLTKQRKRIIQIKGDKRSENETKISNSDNIIMPIPPNEENSLLLTNIKPKNFQNNDNSIQDKEIRLKNPNLISGNGIYDNTY 1643 MNIITLFLYQCCFAFYQIKAGNLCDKLNDFGDNIQTKAILAPIIIIGKGGMKIDLPNAKYKLTIDIVKTIKGSLGEWKSPILMAGEFSTFRDSNN CQFQSQANAEQVYFLEAVSIKNGEFKVLFNPEIYNDLQLDNIYTSICPGCGKTPSVTVKDISKSVDFLVAECMSDGYPAPVTKWKIQKKYAATIIETIKGGVRLIQPINNSTNEVQVCEAVNVIGSASYSIVVKPVIFKTQVVKIKESQIIPCSTNDEKFCNGKGKCYEDKSEKNSKFCRCNKGYMGPNCASVSRSVSNNLVERLKNMDKIFCNNQGISLKNYTTQTIYCECYLNFFGNHCENRYYMKAPVNEGLKEFITEQNSKLAVKYIIIGCLIVLAGCILIVIVIVCLWRFYLTKQRKRIIQIKGDKRSENETKISNSDNIIMPIPPNEENSLLLTNIKPKNFQNNDNSIQDKEIRLKNPNLISGNGIYDNTY Sbjct: 1 MNIITLFLYQCCFAFYQIKAGNLCDKLNDFGDNIQTKAILAPIIIIGKGGMKIDLPNAKYKLTIDIVKTIKGSLGEWKSPILMAGEFSTFRDSNNSCQFQSQANAEQVYFLEAVSIKNGEFKVLFNPEIYNDLQLDNIYTSICPGCGKTPSVTVKDISKSVDFLVAECMSDGYPAPVTKWKIQKKYAATIIETIKGGVRLIQPINNSTNEVQVCEAVNVIGSASYSIVVKPVIFKTQVVKIKESQIIPCSTNDEKFCNGKGKCYEDKSEKNSKFCRCNKGYMGPNCASVSRSVSNNLVERLKNMDKIFCNNQGISLKNYTTQTIYCECYLNFFGNHCENRYYMKAPVNEGLKEFITEQNSKLAVKYIIIGCLIVLAGCILIVIVIVCLWRFYLTKQRKRIIQIKGDKRSENETKISNSDNIIMPIPPNEENSLLLTNIKPKNFQNNDNSIQDKEIRLKNPNLISGNGIYDNTY 473
BLAST of SMED30035435 vs. Planmine SMEST
Match: SMESG000040472.1 (SMESG000040472.1) HSP 1 Score: 855.899 bits (2210), Expect = 0.000e+0 Identity = 472/525 (89.90%), Postives = 472/525 (89.90%), Query Frame = 3 Query: 225 MNIITLFLYQCCFAFYQIKAGNLCDKLNDFGDNIQTKAILAPXXXXXXXXXXXDLPNAKYKLTIDIVKTIKGSLGEWKSPILMAGEFSTFRDSNNFCQFQSQANAEQVYFLEAVSIKNGEFKVLFNPEIYNDLQLDNIYTSICPGCGKTPSVTVKDISKSVDFLVAECMSDGYPAPVTKWKIQKKYAATIIETIKGGVRLIQPINNSTNEVQVCEAVNVIGSASYSIVVKPVIFKTQVVKIKESQIIPCSTNDEKFCNGKGKCYEDKSEKNSKFCRCNKGYMGPNCASVSRSVSNNLVERLKNMDKIFCNNQGISLKNYTTQTIY----------------------------------------------------CECYLNFFGNHCENRYYMKAPVNEGLKEFITEQNSKLAVKYXXXXXXXXXXXXXXXXXXXXXXWRFYLTKQRKRIIQIKGDKRSENETKISNSDNIIMPIPPNEENSLLLTNIKPKNFQNNDNSIQDKEIRLKNPNLISGNGIYDNTY 1643 MNIITLFLYQCCFAFYQIKAGNLCDKLNDFGDNIQTKAILAPIIIIGKGGMKIDLPNAKYKLTIDIVKTIKGSLGEWKSPILMAGEFSTFRDSNN CQFQSQANAEQVYFLEAVSIKNGEFKVLFNPEIYNDLQLDNIYTSICPGCGKTPSVTVKDISKSVDFLVAECMSDGYPAPVTKWKIQKKYAATIIETIKGGVRLIQPINNSTNEVQVCEAVNVIGSASYSIVVKPVIFKTQVVKIKESQIIPCSTNDEKFCNGKGKCYEDKSEKNSKFCRCNKGYMGPNCASVSRSVSNNLVERLKNMDKIFCNNQGISLKNYTTQTIY CECYLNFFGNHCENRYYMKAPVNEGLKEFITEQNSKLAVKYIIIGCLIVLAGCILIVIVIVCLWRFYLTKQRKRIIQIKGDKRSENETKISNSDNIIMPIPPNEENSLLLTNIKPKNFQNNDNSIQDKEIRLKNPNLISGNGIYDNTY Sbjct: 1 MNIITLFLYQCCFAFYQIKAGNLCDKLNDFGDNIQTKAILAPIIIIGKGGMKIDLPNAKYKLTIDIVKTIKGSLGEWKSPILMAGEFSTFRDSNNSCQFQSQANAEQVYFLEAVSIKNGEFKVLFNPEIYNDLQLDNIYTSICPGCGKTPSVTVKDISKSVDFLVAECMSDGYPAPVTKWKIQKKYAATIIETIKGGVRLIQPINNSTNEVQVCEAVNVIGSASYSIVVKPVIFKTQVVKIKESQIIPCSTNDEKFCNGKGKCYEDKSEKNSKFCRCNKGYMGPNCASVSRSVSNNLVERLKNMDKIFCNNQGISLKNYTTQTIYCECYLNFFGDNCEKKFSFSVSKNLVERLKHLDKKYCNNQGISLKNYTTQTIFCECYLNFFGNHCENRYYMKAPVNEGLKEFITEQNSKLAVKYIIIGCLIVLAGCILIVIVIVCLWRFYLTKQRKRIIQIKGDKRSENETKISNSDNIIMPIPPNEENSLLLTNIKPKNFQNNDNSIQDKEIRLKNPNLISGNGIYDNTY 525 The following BLAST results are available for this feature:
BLAST of SMED30035435 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30035435 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30035435 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30035435 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30035435 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30035435 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30035435 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30035435 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 3
BLAST of SMED30035435 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30035435 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30035435 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30035435 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30035435 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30035435 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035435 ID=SMED30035435|Name=SMED30035435|organism=Schmidtea mediterranea sexual|type=transcript|length=1867bpback to top protein sequence of SMED30035435-orf-1 >SMED30035435-orf-1 ID=SMED30035435-orf-1|Name=SMED30035435-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=474bp MNIITLFLYQCCFAFYQIKAGNLCDKLNDFGDNIQTKAILAPIIIIGKGGback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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