Tubulin alpha chain

Overview
NameTubulin alpha chain
Smed IDSMED30035431
Length (bp)1851
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tubulin alpha chain (SMED30035431) t-SNE clustered cells

Violin plots show distribution of expression levels for Tubulin alpha chain (SMED30035431) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30035431

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testisSMED30035431 PL08003A1D07_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30035431 PL08003A1D07_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30035431 PL08003A1D07_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile cDNA to DNA expression microarray evidence
testis primordiumSMED30035431 Contig4533_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1B (tubulin alpha 1b [Source:HGNC Symbol;Acc:HGNC:18809])

HSP 1 Score: 489.189 bits (1258), Expect = 1.926e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1C (tubulin alpha 1c [Source:HGNC Symbol;Acc:HGNC:20768])

HSP 1 Score: 488.804 bits (1257), Expect = 2.004e-168
Identity = 245/434 (56.45%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+A+ +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAVAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1A (tubulin alpha 1a [Source:HGNC Symbol;Acc:HGNC:20766])

HSP 1 Score: 488.804 bits (1257), Expect = 2.034e-168
Identity = 246/434 (56.68%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1A (tubulin alpha 1a [Source:HGNC Symbol;Acc:HGNC:20766])

HSP 1 Score: 488.804 bits (1257), Expect = 2.034e-168
Identity = 246/434 (56.68%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 488.804 bits (1257), Expect = 2.287e-168
Identity = 243/434 (55.99%), Postives = 331/434 (76.27%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEI+D +++ +RK A+ C  LQ F I +SFGGGTGSGF SLL+E+L+V+Y  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CC+ YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-8 (Tubulin alpha-8 chain [Source:UniProtKB/Swiss-Prot;Acc:P52274])

HSP 1 Score: 483.411 bits (1243), Expect = 1.180e-166
Identity = 235/430 (54.65%), Postives = 330/430 (76.74%), Query Frame = 3
Query:   39 KEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            +E V I++GQ+G Q+ NA WEL+CIEHG+   G LKE  ++    + + FF E  NG++VPR I +DLEPTVLDE+R G Y NLFHPDQ+I+G EDAANN+ARGH+T+GKE+ID +++ +RK  E C+ LQ F + +SFGGGTGSGF+SLL+E+L+VE+  + +++ S+  +P ++TSVVEPYN++LT H+++E +D + + DNEAIYD+C + L++ RP+Y+N+NRLIAQ  S +TAS+RF+ +L+ D+++  TNL+PYPRIHFP+ ++AP IS  RA H+PL   +IT M F ++  +V CDP+ GKYM+ CL YRGDV PKDVNA+I  +K +  I FV+WCPTGFK+GIN Q  +TV   ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHWFVGEGMEEGE  EARDD+A L  DY
Sbjct:    6 RECVSIHIGQAGAQIGNACWELYCIEHGLDEAGFLKEEEKNKKQQSLQAFFSESMNGKHVPRAIYVDLEPTVLDEIRNGVYGNLFHPDQIINGKEDAANNYARGHYTIGKELIDVVLDRIRKQTENCEGLQGFLVFHSFGGGTGSGFSSLLMERLSVEFGKKSKLEFSVYPAPQVSTSVVEPYNSILTTHTTLELSDCSFMVDNEAIYDLCRQKLHVERPSYSNLNRLIAQVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLATYAPVISADRAHHEPLSVQDITHMCFEKANQMVKCDPSAGKYMAVCLLYRGDVVPKDVNAAISSVKAKRGINFVDWCPTGFKVGINYQAPITVEGGDLAKVHRAVCMLSNTTAIAEAWARLDHKFDLMYSKRAFVHWFVGEGMEEGEFMEARDDLAALEKDY 435          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-1 (Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:O18688])

HSP 1 Score: 483.026 bits (1242), Expect = 1.975e-166
Identity = 237/435 (54.48%), Postives = 334/435 (76.78%), Query Frame = 3
Query:   24 LIINMKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
             + NM+EV+ I+VGQ+GVQ+ NA WEL+C+EHGIQ +G +  S Q  D  +F TFF +  NG+YVPR I +DLEPTV+DE+RTG+Y+ LFHP+Q+I+G EDAANN+ARGH+T+GKE+ID +++ +R+ A+ C  LQ FF+ +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L++ RP+Y N+NR+I+Q  S +TAS+RF+ +L+ D+++  TNL+PYPRIHFP+ ++ P IS  +A H+ L   +IT   F  +  +V CDP  GKYM+ CL YRGDV PKDVN +I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE  EAR+D+A L  DY
Sbjct:    2 FVFNMREVISIHVGQAGVQIGNACWELYCLEHGIQPDGTMP-SDQQADGESFTTFFSDTGNGRYVPRSIFVDLEPTVVDEIRTGTYKKLFHPEQMITGKEDAANNYARGHYTVGKELIDTVLDRIRRLADNCSGLQGFFVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLSVDRPSYTNLNRIISQVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLAAYTPLISADKAYHEALSVNDITNSCFEPANQMVKCDPRHGKYMAVCLLYRGDVVPKDVNTAIAAIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVPRAVCMLSNTTAIAEAWSRLDYKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALEKDY 435          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-9 (Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:Q20221])

HSP 1 Score: 482.641 bits (1241), Expect = 3.095e-166
Identity = 238/432 (55.09%), Postives = 332/432 (76.85%), Query Frame = 3
Query:   39 KEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKE--SLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            +EV+ I+VGQ+GVQ+ NA WEL+C+EHGIQ +G + E  SL  DD  +F TFF E   G++VPR I +DLEPT +DE+RTG+Y+ LFHP+QL++G EDAANN+ARGH+T+GKEIID +M+ VR+  E C  LQ F + +SFGGGTGSGFT+LL+E+L+V+Y  + +++  I  +P ++TS+VEPYN++LT H+++EH+D + + DNEAIYDIC   L++ RPTY N+NRLIAQ  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ +   EIT+  F     +V CDP  GKYM+CCL YRGDV PKDVNA+I  +K +  I+FV+WCPTGFK+GIN QP   VP +++++  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    7 REVISIHVGQAGVQMGNACWELYCLEHGIQPDGMINEEDSLGVDDD-SFNTFFSETMAGKHVPRAIMVDLEPTPIDEIRTGTYKTLFHPEQLLTGKEDAANNYARGHYTIGKEIIDVVMDRVRRLVENCRGLQGFLVFHSFGGGTGSGFTALLMERLSVDYGKKSKLEFCIYPAPQVSTSMVEPYNSILTTHTTLEHSDCSFMVDNEAIYDICRRNLDLARPTYTNLNRLIAQVISSVTASLRFDGALNVDLTEFQTNLVPYPRIHFPLTTYAPIISAEKAYHEQMTVAEITQQCFEPGAQMVKCDPRRGKYMACCLLYRGDVVPKDVNAAIASVKTKRSIQFVDWCPTGFKVGINYQPPTVVPGADLSKLQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 437          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: mec-12 (Tubulin alpha-3 chain Detyrosinated tubulin alpha-3 chain [Source:UniProtKB/Swiss-Prot;Acc:P91910])

HSP 1 Score: 481.1 bits (1237), Expect = 9.686e-166
Identity = 239/433 (55.20%), Postives = 333/433 (76.91%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL--KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EV+ I++GQ+GVQ+ NA WEL+C+EHGIQ +GQ+   +SL   D  +F TFF E  +G++VPR + +DLEPTV+DE+RTG+YR+LFHP+QLI+G EDAANN+ARGH+T+GKEIID  ++ +R+ A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D + + DNEAIYDIC   L+I RP+Y N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ + +P IS  +A H+ L   EIT M F     +V CDP  GKYM+ CL +RGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MREVISIHIGQAGVQIGNACWELYCLEHGIQPDGQMPSDKSLGGSDD-SFSTFFSETGSGRHVPRAVMVDLEPTVIDEIRTGTYRSLFHPEQLITGKEDAANNYARGHYTIGKEIIDLTLDRIRRLADNCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKAKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCSFMVDNEAIYDICRRNLDIERPSYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATFSPVISAEKAYHEQLSVAEITNMCFEPHNQMVKCDPRHGKYMAVCLLFRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-1 (Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:O18688])

HSP 1 Score: 480.33 bits (1235), Expect = 1.938e-165
Identity = 236/431 (54.76%), Postives = 332/431 (77.03%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EV+ I+VGQ+GVQ+ NA WEL+C+EHGIQ +G +  S Q  D  +F TFF +  NG+YVPR I +DLEPTV+DE+RTG+Y+ LFHP+Q+I+G EDAANN+ARGH+T+GKE+ID +++ +R+ A+ C  LQ FF+ +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L++ RP+Y N+NR+I+Q  S +TAS+RF+ +L+ D+++  TNL+PYPRIHFP+ ++ P IS  +A H+ L   +IT   F  +  +V CDP  GKYM+ CL YRGDV PKDVN +I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE  EAR+D+A L  DY
Sbjct:    1 MREVISIHVGQAGVQIGNACWELYCLEHGIQPDGTMP-SDQQADGESFTTFFSDTGNGRYVPRSIFVDLEPTVVDEIRTGTYKKLFHPEQMITGKEDAANNYARGHYTVGKELIDTVLDRIRRLADNCSGLQGFFVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLSVDRPSYTNLNRIISQVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLAAYTPLISADKAYHEALSVNDITNSCFEPANQMVKCDPRHGKYMAVCLLYRGDVVPKDVNTAIAAIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVPRAVCMLSNTTAIAEAWSRLDYKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALEKDY 430          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub84B (gene:FBgn0003884 transcript:FBtr0081639)

HSP 1 Score: 488.419 bits (1256), Expect = 1.711e-168
Identity = 244/434 (56.22%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEI+D +++ +RK A+ C  LQ F I +SFGGGTGSGFTSLL+E+L+V+Y  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP++++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CC+ YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub85E (gene:FBgn0003886 transcript:FBtr0082087)

HSP 1 Score: 486.493 bits (1251), Expect = 9.313e-168
Identity = 241/434 (55.53%), Postives = 331/434 (76.27%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + +++GQ+GVQ+ NA WEL+C+EHGIQ +G +   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEI+D +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS+ +A H+ L   EIT   F  +  +V CDP  GKYM+CC+ YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE  EAR+D+A L  DY
Sbjct:    1 MRECISVHIGQAGVQIGNACWELYCLEHGIQPDGHMPSDKTVGGGDDS--FSTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDVVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYMNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISVEKAYHEQLTVAEITNACFEPANQMVKCDPRRGKYMACCMLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFAEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub84D (gene:FBgn0003885 transcript:FBtr0081538)

HSP 1 Score: 484.182 bits (1245), Expect = 7.282e-167
Identity = 242/434 (55.76%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEI+D +++ +RK A+ C  LQ F I +SFGGGTGSGFTSLL+E+L+V+Y  + +++ ++  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP++++AP IS  +A H+ L   EIT   F  +  +V  DP  GKYM+CC+ YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKVDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub67C (gene:FBgn0087040 transcript:FBtr0076393)

HSP 1 Score: 452.21 bits (1162), Expect = 3.463e-154
Identity = 235/443 (53.05%), Postives = 323/443 (72.91%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLK--ESLQDDDSIA----------FRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EVV I +GQ G+Q+ NA WEL+ +EHGI  +G LK  E L    S A           RTFF E  NG+ VPR I +DLEPTV+D+VR G  R L+HP+QLISG EDAANN+ARG +++GKE+IDR+ + ++K AE CD LQ F I +S GGGTGSGFTSLL+E+L+ +YS + ++  ++  SP ++T+VVEPYNA+LT HS+M+H+D   + DNEAIYDIC  +L + RP Y N+NRLIAQ  S  TAS+RF  S++ D+++  TNL+P+PRIHFP++++AP +S  R+ H+      +T   F  S ++V CDP  GK+M+CC+ YRGDV PKDVNA++  IK +  I+FV+WCPTGFK+GIN +    VP  ++A+TSR+  MLSNT+AI+ A+ N++ K+DL+F +RAFVHW+VGEGMEEGE  EAR++IA+L  D+
Sbjct:    1 MREVVSIQIGQCGIQIGNACWELYLLEHGINLDGSLKTKEELTASGSSASVGHDTSANDARTFFTETGNGKQVPRSIFVDLEPTVIDDVRNGCMRELYHPEQLISGKEDAANNYARGRYSIGKEVIDRVTSRLQKIAEQCDSLQGFLIFHSLGGGTGSGFTSLLVERLSTDYSKKCKLDFAVYPSPKVSTAVVEPYNALLTTHSTMDHSDCVFMVDNEAIYDICNNSLGVDRPAYRNLNRLIAQIVSSTTASLRFSGSMNVDLNEFQTNLVPFPRIHFPLVAYAPLMSAERSAHEQHAITTLTNACFESSNMMVKCDPRAGKFMACCMLYRGDVVPKDVNAAVSAIKSKRHIQFVDWCPTGFKIGINYEKPAFVPDGDLAKTSRACCMLSNTTAISVAFSNLSYKFDLMFKKRAFVHWYVGEGMEEGEFTEARENIAVLERDF 443          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: CG7794 (gene:FBgn0038565 transcript:FBtr0083540)

HSP 1 Score: 383.645 bits (984), Expect = 1.512e-127
Identity = 196/430 (45.58%), Postives = 292/430 (67.91%), Query Frame = 3
Query:   42 EVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRV-QLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            E++ I++GQ+GVQ+ANA WELFC+EHGI ANG+L +S  DD  + F  F    P  Q  PR++ +D EPTV+DE+RTGSYRNLFHPD LI+G +D+ +NFARG+  +  E++DR MN +R+ A+ C  L+ F +  + GGG+GSG  + ++E+L  ++  +  V +  +  SP ++  +VEPYNA+L  H SM+  D++ + DNEA+YDIC  TLN+P PTY N+NR+IAQ  S  TAS RF    +    +L TNL+PYPRIH+P++++AP + IS ++   + T ++T   F  S  +V C+P+ GKYM+  L YRGD+ P ++N ++ +IK+    +FV+W PTGFK+G++  P   VP  ++A T+R+   +SN + I  AW  +  K+D L+ RRAFV+ +VGEG+EEG  NEA ++I  L+ DY
Sbjct:    6 EIIQIHIGQAGVQIANACWELFCLEHGILANGRLTQSPMDDSFLTFFEFTSHQPCVQ--PRLVMIDTEPTVIDEIRTGSYRNLFHPDTLITGKDDSGSNFARGYNLMASELLDRSMNAIRRVADRCRNLRGFLVFRAIGGGSGSGLGTRIMERLVEDFGKKMTVVEFLVYPSPSISPVIVEPYNALLAAHFSMDCADVSFIVDNEALYDICANTLNVPAPTYTNLNRIIAQVVSSFTASQRFGGGSTVSFQELQTNLVPYPRIHYPLINYAPLVPISHSQFVNMSTAQLTGQCFQMSNQMVRCNPSHGKYMASVLLYRGDIAPNEINTALENIKRNRSFRFVDWSPTGFKIGVSPMPPYYVPGGDLAPTNRACVAISNNTNIRIAWCRLVNKFDKLYQRRAFVYHYVGEGLEEGNFNEASENICQLVHDY 433          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba1c (tubulin, alpha 1c [Source:ZFIN;Acc:ZDB-GENE-061114-1])

HSP 1 Score: 489.189 bits (1258), Expect = 5.596e-169
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba1a (tubulin, alpha 1a [Source:ZFIN;Acc:ZDB-GENE-090507-4])

HSP 1 Score: 489.189 bits (1258), Expect = 1.252e-168
Identity = 246/434 (56.68%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba1c (tubulin, alpha 1c [Source:ZFIN;Acc:ZDB-GENE-061114-1])

HSP 1 Score: 488.804 bits (1257), Expect = 1.591e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba1b (tubulin, alpha 1b [Source:ZFIN;Acc:ZDB-GENE-030822-1])

HSP 1 Score: 487.649 bits (1254), Expect = 3.354e-168
Identity = 245/434 (56.45%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKE+ID +++ VRK ++ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKELIDLVLDRVRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVSEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba1b (tubulin, alpha 1b [Source:ZFIN;Acc:ZDB-GENE-030822-1])

HSP 1 Score: 487.649 bits (1254), Expect = 3.354e-168
Identity = 245/434 (56.45%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKE+ID +++ VRK ++ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKELIDLVLDRVRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVSEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tuba1b (tubulin alpha 4a [Source:NCBI gene;Acc:100038108])

HSP 1 Score: 489.189 bits (1258), Expect = 1.746e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tuba1b (tubulin alpha 4a [Source:NCBI gene;Acc:100038108])

HSP 1 Score: 489.189 bits (1258), Expect = 1.746e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tuba1b (tubulin alpha 4a [Source:NCBI gene;Acc:100038108])

HSP 1 Score: 489.189 bits (1258), Expect = 1.746e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tuba1b (tubulin alpha 4a [Source:NCBI gene;Acc:100038108])

HSP 1 Score: 489.189 bits (1258), Expect = 1.746e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tuba1b (tubulin alpha 4a [Source:NCBI gene;Acc:100038108])

HSP 1 Score: 488.804 bits (1257), Expect = 1.843e-168
Identity = 246/434 (56.68%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba1c (tubulin, alpha 1C [Source:MGI Symbol;Acc:MGI:1095409])

HSP 1 Score: 489.189 bits (1258), Expect = 9.300e-169
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba1b (tubulin, alpha 1B [Source:MGI Symbol;Acc:MGI:107804])

HSP 1 Score: 489.189 bits (1258), Expect = 1.322e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba1a (tubulin, alpha 1A [Source:MGI Symbol;Acc:MGI:98869])

HSP 1 Score: 488.804 bits (1257), Expect = 1.396e-168
Identity = 246/434 (56.68%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba3a (tubulin, alpha 3A [Source:MGI Symbol;Acc:MGI:1095406])

HSP 1 Score: 488.804 bits (1257), Expect = 1.570e-168
Identity = 243/434 (55.99%), Postives = 331/434 (76.27%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEI+D +++ +RK A+ C  LQ F I +SFGGGTGSGF SLL+E+L+V+Y  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CC+ YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba3b (tubulin, alpha 3B [Source:MGI Symbol;Acc:MGI:1095408])

HSP 1 Score: 488.804 bits (1257), Expect = 1.570e-168
Identity = 243/434 (55.99%), Postives = 331/434 (76.27%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEI+D +++ +RK A+ C  LQ F I +SFGGGTGSGF SLL+E+L+V+Y  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CC+ YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|Q3ZCJ7|TBA1C_BOVIN (Tubulin alpha-1C chain OS=Bos taurus OX=9913 GN=TUBA1C PE=1 SV=1)

HSP 1 Score: 489.574 bits (1259), Expect = 5.291e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLISG EDAANN+ARGH+T+GKEIID +++ VRK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRL++Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+A+ +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEIIDLVLDRVRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLMSQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAVAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|P68373|TBA1C_MOUSE (Tubulin alpha-1C chain OS=Mus musculus OX=10090 GN=Tuba1c PE=1 SV=1)

HSP 1 Score: 489.189 bits (1258), Expect = 6.505e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|P68365|TBA1C_CRIGR (Tubulin alpha-1C chain OS=Cricetulus griseus OX=10029 GN=TUBA1C PE=2 SV=1)

HSP 1 Score: 489.189 bits (1258), Expect = 6.505e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|Q6AYZ1|TBA1C_RAT (Tubulin alpha-1C chain OS=Rattus norvegicus OX=10116 GN=Tuba1c PE=1 SV=1)

HSP 1 Score: 489.189 bits (1258), Expect = 8.353e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLARVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|Q6P9V9|TBA1B_RAT (Tubulin alpha-1B chain OS=Rattus norvegicus OX=10116 GN=Tuba1b PE=1 SV=1)

HSP 1 Score: 489.189 bits (1258), Expect = 9.249e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: Q7Z1L8 (Tubulin alpha chain OS=Schmidtea polychroa OX=50054 PE=2 SV=1)

HSP 1 Score: 509.605 bits (1311), Expect = 8.455e-173
Identity = 247/431 (57.31%), Postives = 343/431 (79.58%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            MKE++CIN+GQ+G+Q+ANA WELFC EHGI A+G+++  L D DS AF TFF + P+ QYVPRV+S+DLEPTV+DE+RTG+YR LFHPD LI+G EDAA+NFARGHFT+GK IID  M  +RK  + CD L+ F +++S+GGGTGSGF +L+LEK+ +EY+ R ++ + I   P L+TS VEPYNAVLT H ++E  ++++  DNE++YDIC +TL+I RPTY+N+NRL+AQ +S +T S+R+ESSL++++ Q++TNLIPYPRIHF +++++P +S  RA H+     E+TR VF     L+  +  +GKYMSCCLQYRGD+TP+ VN +I++IK+R+DIKFV+WCPTGFK+GINSQP   +P S +A +  S++M++N++AI   W+N+NKK+ LLF +RAFVHW+VGEGMEEGE NEA D+I+ L+ DY
Sbjct:    1 MKEIICINIGQAGIQMANAIWELFCQEHGITADGKMRSGLTDGDSRAFNTFFFQSPSDQYVPRVLSIDLEPTVVDEIRTGTYRQLFHPDSLINGEEDAASNFARGHFTIGKSIIDVAMAQLRKVVDNCDGLEGFLMISSYGGGTGSGFQTLMLEKIGIEYAKRLKISVVIYPCPKLSTSTVEPYNAVLTSHFTLEQGELSVFFDNESMYDICTKTLDINRPTYSNLNRLLAQIYSAITVSLRYESSLNANLSQIVTNLIPYPRIHFSMVNYSPIVSAQRAAHEQFSVVELTRNVFEPGSQLLRVNMKVGKYMSCCLQYRGDITPRAVNEAIFEIKRRTDIKFVDWCPTGFKIGINSQPPTLIPGSGLAPSKSSVTMITNSTAIVTMWENINKKFTLLFNKRAFVHWYVGEGMEEGEFNEAIDNISYLLKDY 431          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A7M6D9 (Tubulin alpha chain OS=Dugesia ryukyuensis OX=79738 PE=2 SV=1)

HSP 1 Score: 504.212 bits (1297), Expect = 4.424e-170
Identity = 248/431 (57.54%), Postives = 330/431 (76.57%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EV+ INVGQ+G+Q+ANAAWELFCIEHGI+A+G+L++S +   ++ F TFF EVP+G +VPR I++DLEPTV+DE+RT +YR+L+HPD LI+ CEDAANNFARGHFT+GK +I+R ++ +RK    C  +Q F +LNS+GGGTGSG ++L+ E L +EYS   + Q  I  +P L T++VEPYNA+L+   S+EHTD+ +L DNEA ++IC   L+I RP Y N+NRL+AQ FS MT SIRF SSL+ D+ QL TNL+PYPRIHFP +++AP +   RA H+ L   EITR +F  S  L+ CD  +GKYMSCC+QYRG +TP+ VN  +Y+IK+R+DI FV+WCPTGFKLGI+  P   VP SE+A T R+ +M  NT+A+ Q W+ ++KK+DLLF +RAFVHW+VGEGMEEGE  EARDD+  L  DY
Sbjct:    1 MREVISINVGQAGIQMANAAWELFCIEHGIEADGKLRDSERFAQNVGFHTFFQEVPSGNFVPRAINIDLEPTVIDEIRTANYRHLWHPDYLINCCEDAANNFARGHFTVGKNVIERFLDQLRKCVHACQSVQGFIVLNSYGGGTGSGLSALIFEHLDIEYSQSAKFQQCIYPAPQLATAIVEPYNALLSASKSIEHTDVVMLIDNEATFEICTRQLSISRPVYNNLNRLLAQIFSAMTVSIRFGSSLNHDLLQLQTNLVPYPRIHFPAVNYAPILDPHRANHEKLSITEITRELFESSNQLIKCDLRLGKYMSCCIQYRGLITPRQVNLGVYEIKRRTDISFVDWCPTGFKLGISPMPPTIVPGSEIAPTERNCTMFCNTTAVAQVWQRLDKKFDLLFNKRAFVHWYVGEGMEEGEFVEARDDLIKLEKDY 431          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A310SSF3 (Tubulin alpha chain (Fragment) OS=Eufriesea mexicana OX=516756 GN=WN48_08017 PE=3 SV=1)

HSP 1 Score: 498.049 bits (1281), Expect = 7.212e-169
Identity = 243/435 (55.86%), Postives = 333/435 (76.55%), Query Frame = 3
Query:   27 IINMKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL-KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            +I  +EV+ +++GQ GVQ+ NA WEL+C+EHGIQ +G L  +S  +DD   F+TFF E   G+YVPR + +DLEPTV+DEVRTG+Y  LFHP+QLISG EDAANN+ARGH+TLGKEIID +++ +RK A+ C  LQ F I ++FGGGTGSGFTSLL+E+L+++Y  + +++ +I  SP ++T+VVEPYNA+LT H+++EH+D A L DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP +SI +A H+ L   E+T   F  +  +V C+P  GKYM+CCL YRGDV PKDVNASI  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R+++ML+NT+AI +AW  +N+K+DL+F +RAFVHW++ E M+E E NEAR+D+A L  DY
Sbjct:    2 LITKREVLSVHIGQGGVQMGNACWELYCLEHGIQPDGMLPAQSCTNDD--GFQTFFSETGGGKYVPRAVFLDLEPTVIDEVRTGTYHQLFHPEQLISGKEDAANNYARGHYTLGKEIIDLVLDRIRKIADMCTGLQGFLIFHAFGGGTGSGFTSLLMERLSMDYGKKAKLEFAIYPSPQISTAVVEPYNAILTTHTTLEHSDCAFLVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPIVSIEKAYHEQLTVMELTSSCFEPAMQMVKCNPQRGKYMACCLLYRGDVVPKDVNASIATIKTKRAIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAMAMLANTTAIAEAWTRLNRKFDLMFGKRAFVHWYITESMDESEFNEAREDLAALEKDY 434          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A1X2GXA8 (Tubulin alpha chain OS=Hesseltinella vesiculosa OX=101127 GN=DM01DRAFT_1315646 PE=3 SV=1)

HSP 1 Score: 496.893 bits (1278), Expect = 2.460e-168
Identity = 243/432 (56.25%), Postives = 343/432 (79.40%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL-KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EV+ I+VGQ+GVQ+ NA WEL+C+EHGIQ +G++  E+L  D S  F TFF E  +G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKE++D +++ VRK A+ C  LQ F + +SFGGGTGSGF +LL+E+L+V+Y  + +++ S+  +P ++TSVVEPYN++LT H+++EH+D++ + DNEAIYDIC   L+I RPTY+N+NRLIAQ  S +TAS+RF+ SL+ D+++  TNL+PYPRIHFP++++AP +S ++A H+ L   EIT   F  +  +V CDP  GKYMSCCL YRGDV PKD N++I +IK +  I+FV+WCPTGFK+GIN +P  TVP +++AQ +R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MREVISIHVGQAGVQIGNACWELYCLEHGIQPDGRIPDENLAGDHS--FDTFFSETDSGKHVPRTVFVDLEPTVVDEVRTGAYRQLFHPEQLITGKEDAANNYARGHYTVGKELVDSVLDRVRKLADGCTGLQGFLVFHSFGGGTGSGFGALLMERLSVDYGKKSKLEFSVYPAPQVSTSVVEPYNSILTTHTTLEHSDVSFMVDNEAIYDICRRNLDIERPTYSNLNRLIAQVVSSITASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVTYAPIVSAAKAYHEQLSVPEITSACFEPNNQMVKCDPRQGKYMSCCLLYRGDVVPKDTNSAIANIKTKRTIQFVDWCPTGFKVGINCKPPTTVPGADLAQVNRALCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 430          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A0L7QLG6 (Tubulin alpha chain (Fragment) OS=Habropoda laboriosa OX=597456 GN=WH47_10606 PE=3 SV=1)

HSP 1 Score: 496.123 bits (1276), Expect = 3.655e-168
Identity = 242/431 (56.15%), Postives = 331/431 (76.80%), Query Frame = 3
Query:   39 KEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLK-ESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            +EV+ +++GQ GVQ+ NA WEL+C+EHGIQ +G L  +S   D+   F+TFF E   G+YVPR + +DLEPTV+DEVRTG+Y  LFHP+QLISG EDAANN+ARGH+TLGKEIID +++ +RK+A+ C  LQ F I ++FGGGTGSGFTSLL+E+L+++Y  + +++ +I  SP ++T+VVEPYNA+LT H+++EH+D A L DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP +SI +A H+ L   E+T   F  +  +V C+P  GKYM+CCL YRGDV PKDVNASI  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R+++ML+NT+AI +AW  +N+K+DL+F +RAFVHW+V E M+E E NEAR+D+A L  DY
Sbjct:    2 REVLSVHIGQGGVQMGNACWELYCLEHGIQPDGMLPPQSCSSDE--GFQTFFSETGGGKYVPRAVFLDLEPTVIDEVRTGTYHQLFHPEQLISGKEDAANNYARGHYTLGKEIIDLVLDRIRKTADMCSGLQGFLIFHAFGGGTGSGFTSLLMERLSMDYGKKAKLEFAIYPSPQISTAVVEPYNAILTTHTTLEHSDCAFLVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPIVSIEKAYHEQLTVMELTSSCFEPAMQMVKCNPQRGKYMACCLLYRGDVVPKDVNASIATIKTKRAIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAMAMLANTTAIAEAWTRLNRKFDLMFGKRAFVHWYVQESMDESEFNEAREDLAALEKDY 430          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: ENSAMXT00000049713.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:103046666])

HSP 1 Score: 489.189 bits (1258), Expect = 9.941e-169
Identity = 246/434 (56.68%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: ENSAMXT00000030346.1 (tubulin alpha-1B chain [Source:NCBI gene;Acc:111195381])

HSP 1 Score: 488.804 bits (1257), Expect = 1.348e-168
Identity = 246/434 (56.68%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: si:ch211-114n24.6 (tubulin alpha-1A chain-like [Source:NCBI gene;Acc:103034569])

HSP 1 Score: 486.493 bits (1251), Expect = 1.029e-167
Identity = 244/434 (56.22%), Postives = 331/434 (76.27%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + ++VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++  RK A+ C  LQ F I +SFGGGTGSGFTSLL+E+L+V+Y  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVN++I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNSAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: tuba8l2 (tubulin alpha chain-like [Source:NCBI gene;Acc:103042625])

HSP 1 Score: 486.108 bits (1250), Expect = 1.168e-167
Identity = 244/434 (56.22%), Postives = 331/434 (76.27%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + ++VGQ+GVQ+ N  WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G+YVPR I +DLEPTV+DEVRTG+YR LFHP+QLISG EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V++  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RP+Y N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  S  +V CDP  GKYM+CCL YRGDV PKDVN +I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISVHVGQAGVQMGNTCWELYCLEHGIQPDGQMPSQKPVGGHDDS--FTTFFSETGAGKYVPRAIFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEIIDSVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDFGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPSYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNSCFEPSNQMVKCDPRHGKYMACCLLYRGDVVPKDVNVAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: tuba1a (tubulin alpha chain [Source:NCBI gene;Acc:103037973])

HSP 1 Score: 486.108 bits (1250), Expect = 1.490e-167
Identity = 245/434 (56.45%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ VRK ++ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RP+Y N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRVRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPSYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVSEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 404.06 bits (1037), Expect = 1.109e-137
Identity = 199/353 (56.37%), Postives = 269/353 (76.20%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGF 1085
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEI+D +++ +RK A+ C  LQ F I +SFGGGTGSGFTSLL+E+L+V+Y  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CC+ YRGDV PKDVNA+I  IK +  I+FV+WCPTGF
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGF 351          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001139.1 (pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 gene:ENSPMAG00000001018.1 transcript:ENSPMAT00000001139.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 320.087 bits (819), Expect = 7.659e-105
Identity = 167/355 (47.04%), Postives = 245/355 (69.01%), Query Frame = 3
Query:   39 KEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSI---AFRTFFHEVPNGQYVPRVISMDLEPTVLDE---VRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGF 1085
            ++ + I++GQ+GVQ+ NA WEL+C+EHGI+ +G L  +  D  S+   +F TFF E  +G++VPR I +DLEP+V+ E   +RT +         +++G E  ++   RG    G++I  R++   R  +E C  LQ F + +SFGGGTGSGFTSLL+E+L+ +Y  + +++  I  +P ++T+VVEPYNA+LT H+++EH+D + + DNEAIYDIC + L+I  P+Y N+NRLIAQ  S +TAS+RF+ +L+ D+++  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  S  +V CDP  GKYM+CCL YRGD+ PKDVN +I  IK R  ++FV+WCPTGF
Sbjct:    3 RDCISIHIGQAGVQMGNACWELYCLEHGIKPDGHLA-THPDVTSVVDSSFNTFFSETGSGKHVPRAIFIDLEPSVIGEPCGLRTLTVGGXLRMSMMLAGREVRSSRIWRGQKGGGRKICSRVLRRNRPQSERCTGLQGFLLFHSFGGGTGSGFTSLLMERLSADYGKKSKLEFCIYPAPQVSTAVVEPYNAILTTHTTLEHSDCSFMVDNEAIYDICRKKLDIDFPSYFNLNRLIAQMVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLTTYAPVISAEKAYHEQLSVAEITNACFEPSNQMVKCDPRNGKYMACCLLYRGDIVPKDVNTAIAIIKSRRAVRFVDWCPTGF 356          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: tubb2 (tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GENE-050522-384])

HSP 1 Score: 250.751 bits (639), Expect = 6.601e-78
Identity = 135/387 (34.88%), Postives = 225/387 (58.14%), Query Frame = 3
Query:   96 WELFCIEHGIQANGQLKESLQDDDSIAFR---TFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICIS---PVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRR 1238
            WE+   EHGI   G    S   D  +       +++E   G+YVPR I +DLEP  +D VR+G +  +F PD  + G   A NN+A+GH+T G E++D +++VVRK AE CD LQ F + +S GGGTGSG  +LL+ K+  EY    +V  ++  S   P ++ +VVEPYNA L++H  +E+TD     DNEA+YDIC  TL +  PTY ++N L++   S +T  +RF   L++D+ +L  N++P+PR+HF +   AP  S    +++ L   E+T+ +F    ++  CDP  G+Y++    +RG ++ K+V+  + +++ ++   FVEW P   K  +       +P   +     S + + N++AI + +K +++++  +F R+
Sbjct:    3 WEVISDEHGIDPTG----SYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYI--IQVLYTVVASGPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAV-----CDIPPRGLKM---SATFIGNSTAIQELFKRISEQFTAMFRRK 375          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005307.1 (pep scaffold:Pmarinus_7.0:GL485425:14485:17012:-1 gene:ENSPMAG00000004835.1 transcript:ENSPMAT00000005307.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 158.303 bits (399), Expect = 3.722e-46
Identity = 74/131 (56.49%), Postives = 99/131 (75.57%), Query Frame = 3
Query:   39 KEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRK--SAEC 416
            +E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+  VRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEI+D +++ +RK  S  C
Sbjct:    2 RECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSEKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVGAVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLVSGSC 130          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001368.1 (pep scaffold:Pmarinus_7.0:GL476337:866089:875956:-1 gene:ENSPMAG00000001230.1 transcript:ENSPMAT00000001368.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 165.622 bits (418), Expect = 4.501e-45
Identity = 127/447 (28.41%), Postives = 214/447 (47.87%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHG-IQANGQLKESLQDDDSIAFRTFFHEVPNGQYV--------------PRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDI-------------------------CLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWF 1256
            M + V + VGQ G Q+    W+L   EH  +   G   E+L    S  FR        G+ V               R + +D+E  V++E+  G  R++F   QLI+    + NN+A G+   G +  ++I+  +R +AE CD LQ FF+++S GGGTGSG  + +L  L  E+ +  R  +++  S      V  PYN+VL +    EH D  +  +N+A+ DI                         CL+    P   +  +N ++A    +MT+S RFE SL+ D++++  NL+P+PR+H+ V S  P  +++     P    ++    F +   L+  DP    Y++C L  RG+V   DV  +I  +K    + FV+W   G+K G+ S P V  P S +A        L+N + +   + ++ +++  L+ R+A +H +
Sbjct:    1 MAQSVVVQVGQCGNQIGCRFWDLALREHAAVNKKGIYDEAL----SSFFRNVDTRPGTGEEVDIPVDSRSTIRSLKARAVLIDMEEGVVNELLQGPLRDVFDQKQLITDVSGSGNNWAVGNKVYGAQFREKILERLRVTAEHCDSLQCFFLIHSMGGGTGSGLGTFVLRLLEDEFPDVHRFVMALYPSAE-DDVVTSPYNSVLAMRELTEHADCVLPIENQALVDIVNKINHAAALGKSVRSQTTLSSSHGCLKGQEKP---FDTMNNIVANLMLNMTSSSRFEGSLNVDLNEITMNLVPFPRLHYLVSSLTPLYALADVALPPRRLDQMFSDAFSKENQLLRVDPRRSLYLACALMVRGNVQLSDVRRNIERLK--PSLHFVDWNEEGWKTGLCSVPPVGYPHSLLA--------LANNTCVKHTFADIQERFARLYRRKAHLHHY 429          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB3 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication [Source:SGD;Acc:S000004593])

HSP 1 Score: 461.07 bits (1185), Expect = 9.309e-159
Identity = 229/433 (52.89%), Postives = 314/433 (72.52%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQD--DDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EV+ INVGQ+G Q+ NA WEL+ +EHGI+ +G L++ L         F TFFHE   G++VPR I +DLEP V+DEVRTG ++ LFHP+QLI+G EDAANN+ARGH+T+G+EI+D +   +RK A+ CD LQ F   +S GGGTGSG  SLLLE L+ EY  + +++ ++  +P L+TSVVEPYN VLT H+++EH D   + DNEAIYDIC   L I RP+++N+N LIAQ  S +TAS+RF+ SL+ D+++  TNL+PYPRIHFP++S+AP +S  RA H+     EIT   F     +V CDPT GKYM+ CL YRGDV  +DV  ++  +K +  ++ V+WCPTGFK+GI  +P   +P+SE+A   R++ MLSNT+AI  AWK +++K+DL++ +RAFVHW+VGEGMEEGE  EAR+D+A L  DY
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKEDGHLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAANNYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLENLSYEYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNLGISRPSFSNLNGLIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPILSKKRATHESNSVSEITNACFEPGNQMVKCDPTKGKYMANCLLYRGDVVTRDVQRAVEQVKNKKTVQMVDWCPTGFKIGICYEPPSVIPSSELANVDRAVCMLSNTTAIADAWKRIDQKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB1 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication [Source:SGD;Acc:S000004550])

HSP 1 Score: 451.825 bits (1161), Expect = 4.492e-155
Identity = 225/433 (51.96%), Postives = 317/433 (73.21%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQD--DDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EV+ INVGQ+G Q+ NA WEL+ +EHGI+ +G L++ L         F TFFHE   G++VPR I +DLEP V+DEVR G Y++LFHP+QLISG EDAANN+ARGH+T+G+EI+  +++ +RK A+ CD LQ F   +S GGGTGSG  SLLLE+L+ EY  + +++ ++  +P ++TSVVEPYN VLT H+++EH D   + DNEAIYD+C   L+IPRP++AN+N LIAQ  S +TAS+RF+ SL+ D+++  TNL+PYPRIHFP++S++P +S S+A H+     EIT   F     +V CDP  GKYM+ CL YRGDV  +DV  ++  +K +  ++ V+WCPTGFK+GI  +P    P S++A   R++ MLSNT++I +AWK +++K+DL++ +RAFVHW+VGEGMEEGE  EAR+D+A L  DY
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKPDGHLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRNGPYKDLFHPEQLISGKEDAANNYARGHYTVGREILGDVLDRIRKLADQCDGLQGFLFTHSLGGGTGSGLGSLLLEELSAEYGKKSKLEFAVYPAPQVSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCKRNLDIPRPSFANLNNLIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYSPVLSKSKAFHESNSVSEITNACFEPGNQMVKCDPRDGKYMATCLLYRGDVVTRDVQRAVEQVKNKKTVQLVDWCPTGFKIGICYEPPTATPNSQLATVDRAVCMLSNTTSIAEAWKRIDRKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB2 (Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria [Source:SGD;Acc:S000001857])

HSP 1 Score: 295.434 bits (755), Expect = 3.736e-94
Identity = 160/431 (37.12%), Postives = 259/431 (60.09%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E++ I+ GQ G Q+  A WE  C EHG+  NG       D        +F+E  +G++VPR I++DLEP  +D VR  +  NLF PD  I G   A N +A+GH+T G E++D +M+V+R+ AE CD LQ F I +S GGGTGSG  +LL+ K+  E+ +R     S+  SP  + +VVEPYNA L++H  +EH+D     DNEA+YDIC  TL + +P+Y ++N L++   S +T S+R+   L+SD+ +L  NL+P+PR+HF ++ +AP  +I     + L   E+T+ +F    ++   DP  G+Y++    +RG V+ K+V   ++ ++ ++   FVEW P   +  + S   V     +MA T      ++N+++I + +K V  ++  +F R+AF+HW+  EGM+E E +EA  ++  L+++Y
Sbjct:    1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYH-GHDDIQKERLNVYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPELTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAVCS---VAPQGLDMAAT-----FIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEFSEAESNMNDLVSEY 422          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB4 (Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004202])

HSP 1 Score: 122.094 bits (305), Expect = 4.346e-30
Identity = 119/417 (28.54%), Postives = 197/417 (47.24%), Query Frame = 3
Query:   42 EVVCINVGQSGVQLANAAWELFCIEHGIQANG--QLKESL--QDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQ--LISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVV-EPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNIS------ISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQ-PNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVH 1250
            E++ +  GQ G  +    W     EH I  +G  QL +S   +DDD+   + FF E    ++ PR I MD EP+V+ +V   ++R  F P    + S    A N++A G + +G    D I+N + K  +  D  + F +L+S  GGTGSG  S LLE L   Y  +     S+   P  ++ VV + YN +L +   +E +D  ++ DN ++ +I  +    P     + N+LI+   S +T SIRF S + S M  + + LIP P +HF   S  P  S      I+   H   D   +   +   S  LV+       Y +      G+V P+ ++ ++  +++R  IKF  W  +   + I  + P + +  +E      S  MLSN S +   ++N    +D +F + AF++
Sbjct:    4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDT---KPFFRENSRNKFTPRAIMMDSEPSVIADVEN-TFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVF--PARSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYD---VMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHVNIGRRSPYLPLQPNE---NEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLN 405          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO38067 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDY3])

HSP 1 Score: 487.649 bits (1254), Expect = 1.137e-168
Identity = 244/434 (56.22%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKE+ID +++ +RK A+ C  LQ F I +SFGGGTGSGF+SLL+E+L+V+Y  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKELIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFSSLLMERLSVDYGKKSKLEFAIYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO44803 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUT0])

HSP 1 Score: 487.649 bits (1254), Expect = 1.447e-168
Identity = 245/434 (56.45%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F I +SFGGGTGSGFTSLL+E+L+V+Y  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CC+ YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO44804 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUT1])

HSP 1 Score: 487.649 bits (1254), Expect = 1.495e-168
Identity = 244/434 (56.22%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEI+D +++ +RK A+ C  LQ F I +SFGGGTGSGF+SLL+E+L+V+Y  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFSSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO38091 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDY0])

HSP 1 Score: 487.263 bits (1253), Expect = 1.681e-168
Identity = 244/434 (56.22%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKE+ID +++ +RK A+ C  LQ F I +SFGGGTGSGF+SLL+E+L+V+Y  + +++ +I  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKELIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFSSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO41766 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3G2])

HSP 1 Score: 486.108 bits (1250), Expect = 4.836e-168
Identity = 239/432 (55.32%), Postives = 332/432 (76.85%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL-KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+  +  Q     +F TFF E   G++VPR + +DLEPTV+DEVRTGSYR LFHP+QLI+G EDAANN+ARGH+T+GK+ +D++++ +RK A+ C  LQ FFI +SFGGGTGSGF+SLL+E+L+V+Y  + +++ ++  +P + ++VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RP+Y N+NRLI Q  S +TAS+RF+ SL+ D+++  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  LV CDP  GKYM+CCL +RGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTQGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGSYRQLFHPEQLITGKEDAANNYARGHYTIGKQQVDQVLDRLRKLADQCSGLQGFFIFHSFGGGTGSGFSSLLMERLSVDYGKKSKLEFAVYPAPHIASAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPSYTNLNRLIGQIVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQLVKCDPRHGKYMACCLLFRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000037900.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 490.73 bits (1262), Expect = 2.538e-169
Identity = 247/434 (56.91%), Postives = 332/434 (76.50%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYNA+LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000033163.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 490.345 bits (1261), Expect = 2.860e-169
Identity = 247/434 (56.91%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYNA+LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000020578.2 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 490.345 bits (1261), Expect = 2.860e-169
Identity = 247/434 (56.91%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYNA+LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000020614.2 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 488.419 bits (1256), Expect = 1.847e-168
Identity = 246/436 (56.42%), Postives = 333/436 (76.38%), Query Frame = 3
Query:   30 INMKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            ++ +E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYNA+LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL YRGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MSKRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 434          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: tuba1c (tubulin alpha-1B chain-like [Source:NCBI gene;Acc:101167538])

HSP 1 Score: 487.263 bits (1253), Expect = 5.158e-168
Identity = 245/434 (56.45%), Postives = 333/434 (76.73%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQL---KESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+E + I+VGQ+GVQ+ NA WEL+C+EHGIQ +GQ+   K     DDS  F TFF E   G++VPR + +DLEPTV+DEVRTG+YR LFHP+QLI+G EDAANN+ARGH+T+GKEIID +++ +RK A+ C  LQ F + +SFGGGTGSGFTSLL+E+L+V+Y  + +++ SI  +P ++T+VVEPYN++LT H+++EH+D A + DNEAIYDIC   L+I RPTY N+NRLI+Q  S +TAS+RF+ +L+ D+ +  TNL+PYPRIHFP+ ++AP IS  +A H+ L   EIT   F  +  +V CDP  GKYM+CCL +RGDV PKDVNA+I  IK +  I+FV+WCPTGFK+GIN QP   VP  ++A+  R++ MLSNT+AI +AW  ++ K+DL++ +RAFVHW+VGEGMEEGE +EAR+D+A L  DY
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTLGGGDDS--FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLFRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDY 432          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000009421.1 (SMESG000009421.1)

HSP 1 Score: 555.444 bits (1430), Expect = 0.000e+0
Identity = 270/431 (62.65%), Postives = 357/431 (82.83%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M E++CIN GQ+GVQL+NAAWELFCIEHGIQANG LKE+L++ DS+AF+TFF EVP GQYVPR IS+DLEPTVLDEVRTG+YRNL+HPDQLISG EDAANN+ARGHFTLGK +ID  +  +R++ + CD LQ FFI+NSFGGGTGSGF +LL+E++ VEY+ R + Q+ I  S  + T+VVEPYN++L+++SS EH +I  +TDNEA+Y +   TL+I RPTY N+NR++AQ FS ++ S+R+ES L++D DQ+ITNL+PYPRIH+PV +++P +S+ RAEH+ L T EITR +F     +V+   + G+YM+CCLQYRG+VTPKDVN ++++IK+R+D+KFVEWCPTGFKLGIN QP   VP SE+AQT RS++MLSNT+A++Q W N+ +K+D+LF +R+FVHW+VGEGMEEGE NEA+D++  LI DY
Sbjct:    1 MPEIICINTGQAGVQLSNAAWELFCIEHGIQANGLLKENLRNGDSMAFKTFFQEVPGGQYVPRAISIDLEPTVLDEVRTGAYRNLWHPDQLISGSEDAANNYARGHFTLGKLLIDATVERIRRAVDMCDRLQGFFIINSFGGGTGSGFGALLIERIGVEYTKRPKFQVVIVPSNHMATAVVEPYNSLLSLNSSTEHAEICNMTDNEAVYQLLTNTLDISRPTYNNLNRILAQVFSSLSVSLRYESLLNADFDQIITNLVPYPRIHYPVCNYSPIVSVRRAEHEQLSTHEITRSIFDDQSQMVSIKISDGRYMACCLQYRGNVTPKDVNNAVFEIKRRTDVKFVEWCPTGFKLGINCQPPTAVPGSELAQTIRSVTMLSNTTAVSQIWGNIGRKFDVLFSKRSFVHWYVGEGMEEGEFNEAKDEMTSLIQDY 431          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000019721.1 (SMESG000019721.1)

HSP 1 Score: 523.857 bits (1348), Expect = 0.000e+0
Identity = 261/431 (60.56%), Postives = 339/431 (78.65%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EV+CI+VGQ+G+Q++NAAWELFCIEHGI ANG LKESL+DDDS AF+TFF E PNGQ+VPR +++DLEP+V+DE+R GSYRNLFHP QL++  +D +NNFAR +FT+GK      ++V+RK  E CD LQ FF+L+S  GGTGSGFTSL+L KL+ E++   + Q SI  SPVL ++VVEPYN  L     +EH+DI+ +  NEA+++IC   L + RPTY+NINR+I+  FSD+T S+RFE+SL+S+  Q+  NLIPYPRIHFP+MSHAP +S   AEH+ L T +ITR VF     L+TCD   G YM C LQ+RGDVTPKDVN +++ +K++S+IKFV+WCPTGFKLGINSQP VTVP+SEMAQ+ RS++M+SNT+ I    + V KK+D L Y+R+FVHWFVGEGMEEGE NEARD++A LI D+
Sbjct:    1 MQEVICIHVGQAGIQMSNAAWELFCIEHGINANGLLKESLKDDDSTAFKTFFCEAPNGQFVPRALTVDLEPSVIDEIRVGSYRNLFHPKQLLNVFDDGSNNFARAYFTVGKSFYLETLDVLRKITENCDGLQGFFLLHSHAGGTGSGFTSLILNKLSTEFAKTMKFQFSIFPSPVLCSAVVEPYNTTLAAQYDVEHSDISTIISNEAVFNICNNQLAVSRPTYSNINRVISHGFSDLTVSLRFENSLTSNFAQMGVNLIPYPRIHFPLMSHAPLMSKLMAEHEKLSTDQITRSVFDAKNQLITCDVLNGLYMCCVLQFRGDVTPKDVNQAVFKLKRKSEIKFVDWCPTGFKLGINSQPKVTVPSSEMAQSPRSVTMISNTTGIKDPMEIVMKKFDTLLYKRSFVHWFVGEGMEEGEFNEARDEMANLILDF 431          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000057127.1 (SMESG000057127.1)

HSP 1 Score: 520.005 bits (1338), Expect = 5.841e-180
Identity = 258/431 (59.86%), Postives = 332/431 (77.03%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EVVCINVGQ+G+Q+ANA+WELFCIEHGI+ NG+LK   QD  S  F TFF EVP+G YVPR I++DLEPTV+DE+RT SYRNL+HPD LI+ CEDAANNFARGH+T+GK +I++ M  +RK A  CD +Q F +LNS+GGGTGSG ++L+ E L+VE+S   + Q +I  +P L T++VEPYN++LT    ME+TD+ +L DNEA YDIC + LNI RP Y NINRLIAQ FS MT SIRF +SL +D+ QL TNLIPYPRIHFP ++++P I   RA H+ L   EITR +F     L+ CD  +GKYMSCC+QYRG +TP+ VN ++YDIK+R++I FV+WCPTGFKLGI+  P  TVP SEMA+T ++ +M  NTSA+ Q W+ ++KK+DLLF++RAF+HW+VGEGMEE E  EAR D+  L  DY
Sbjct:    1 MREVVCINVGQAGIQMANASWELFCIEHGIECNGKLKYDAQDFTS--FHTFFQEVPSGNYVPRAINVDLEPTVIDEIRTASYRNLWHPDYLINCCEDAANNFARGHYTIGKSVIEKFMERLRKCAMACDSVQGFIVLNSYGGGTGSGLSALIFENLSVEFSKTAKFQQAIYPAPQLATAIVEPYNSLLTASKCMENTDVVMLIDNEATYDICFKKLNISRPGYNNINRLIAQIFSAMTVSIRFGASLDNDLVQLQTNLIPYPRIHFPTVNYSPIIDSHRAAHEGLTISEITRELFDPGNQLIRCDLRLGKYMSCCIQYRGLITPRQVNIAVYDIKRRTEINFVDWCPTGFKLGISPMPPATVPGSEMAKTDKNCTMFCNTSAVAQLWQRLDKKFDLLFHKRAFIHWYVGEGMEEAEFVEARSDLVALEQDY 429          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000057667.1 (SMESG000057667.1)

HSP 1 Score: 510.375 bits (1313), Expect = 1.511e-176
Identity = 255/431 (59.16%), Postives = 333/431 (77.26%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EVV INVGQ+GVQ+ NA WELFC EH I  NG      +     AF+T FHEVPNGQYVPR +S+DLEPTVLDEVRTG YRNLFHPDQLISGCEDAANN+ARGHFT+G++IID+ +N +RK A  C Y+Q F +++S  GGTG+GF++LL E+L++E++ R + Q+++  +P L TSVV PYN V+  H+SM+  +++++ DNEA+YDIC + L + RPT++N+NRL+AQ  S MT S+RFE+SL+SD+DQL TNLIP+PRIHFP   HAP    S+ EH+ +D  E+TR +F  S   +  +  +GK MSC LQYRG   PK VN +I++IK+R+DI FV+WCPTGFK+GINS P  TVP S +AQTSRS++MLSN++AI Q W  +NKK+DLLF +R+FVHWFVGEGMEEGE NEARD++  +I DY
Sbjct:    1 MREVVFINVGQAGVQIGNAIWELFCSEHNIGPNGFPFNEAEISGDEAFQTIFHEVPNGQYVPRAVSVDLEPTVLDEVRTGCYRNLFHPDQLISGCEDAANNYARGHFTVGRKIIDQCVNQIRKVAHACTYIQGFVLVDSMAGGTGAGFSTLLNERLSIEFTKRPKFQVAVYPAPKLDTSVVSPYNTVMNTHASMDFIELSVIMDNEALYDICFDCLLVQRPTFSNLNRLVAQVLSSMTVSLRFENSLNSDLDQLCTNLIPFPRIHFPTSHHAPVECQSKVEHETIDEIELTRSLFLPSSQFIKVNCGMGKLMSCVLQYRGQCNPKSVNEAIFEIKRRNDIHFVDWCPTGFKVGINSFPPTTVPGSGLAQTSRSVTMLSNSTAICQMWVKLNKKFDLLFAKRSFVHWFVGEGMEEGEFNEARDNLDSIIKDY 431          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000010593.1 (SMESG000010593.1)

HSP 1 Score: 508.449 bits (1308), Expect = 1.434e-175
Identity = 251/431 (58.24%), Postives = 341/431 (79.12%), Query Frame = 3
Query:   36 MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRTFFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAANNFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNXXXXXXXXXXXXXXXEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIAILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSDMDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCILVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGFKLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYRRAFVHWFVXXXXXXXXXXXARDDIAILIADY 1328
            M+EV+CIN+GQ+GVQL+NAAWELFC+EHGI+ANG LKE+L  D++ +FRTFF EVP GQ+VPR IS+DLEPTV+DE+R+G YRNLFHPDQLIS  ED A+NFAR  FT+GK  I+ + N VRK  + C+ LQ FF+++S  GGTGSGFT+L L+ L +EY+ R + Q+S+  SP+L+T+VVEP+N  L+   ++E+ DI  + DNEAIY+I    LNIPRPT++NINR+I+Q FSDMT S+RFE SL+SD++QL TNLIPYPRIHFPV  H P  S S+AEH+PL T  +T+ +F ++  +V+C    GKYM C L YRG +TPK+VN +I++IK++++IKFV+WCPTGFKLGIN+QP +  P SE+A + +S++ML+N+++I   W+ +  K+D+LF +R+FVHW+VGEGMEEGE NEARD++  +  DY
Sbjct:    1 MREVICINIGQAGVQLSNAAWELFCMEHGIEANGLLKENLSLDETDSFRTFFQEVPTGQFVPRTISIDLEPTVIDEIRSGPYRNLFHPDQLISSNEDGASNFARAFFTIGKRQIENLTNTVRKLVDACEMLQGFFLIHSVSGGTGSGFTTLALDTLTLEYTKRNKFQMSVFPSPMLSTAVVEPFNTSLSSVRALENCDITNIMDNEAIYNISNACLNIPRPTFSNINRVISQVFSDMTTSLRFEGSLNSDLNQLCTNLIPYPRIHFPVCCHTPLTSPSKAEHEPLTTATLTKNLFDKNSQMVSCTLNEGKYMCCVLMYRGRITPKEVNQAIFEIKRKNEIKFVDWCPTGFKLGINNQPPIMAPGSEIAPSDKSVTMLANSTSIKDIWEKIMSKFDILFSKRSFVHWYVGEGMEEGEFNEARDELNGIYLDY 431          
The following BLAST results are available for this feature:
BLAST of Tubulin alpha chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBA1B1.926e-16856.68tubulin alpha 1b [Source:HGNC Symbol;Acc:HGNC:1880... [more]
TUBA1C2.004e-16856.45tubulin alpha 1c [Source:HGNC Symbol;Acc:HGNC:2076... [more]
TUBA1A2.034e-16856.68tubulin alpha 1a [Source:HGNC Symbol;Acc:HGNC:2076... [more]
TUBA1A2.034e-16856.68tubulin alpha 1a [Source:HGNC Symbol;Acc:HGNC:2076... [more]
TUBA3C2.287e-16855.99tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tba-81.180e-16654.65Tubulin alpha-8 chain [Source:UniProtKB/Swiss-Pro... [more]
tba-11.975e-16654.48Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:... [more]
tba-93.095e-16655.09Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:... [more]
mec-129.686e-16655.20Tubulin alpha-3 chain Detyrosinated tubulin alpha-... [more]
tba-11.938e-16554.76Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
alphaTub84B1.711e-16856.22gene:FBgn0003884 transcript:FBtr0081639[more]
alphaTub85E9.313e-16855.53gene:FBgn0003886 transcript:FBtr0082087[more]
alphaTub84D7.282e-16755.76gene:FBgn0003885 transcript:FBtr0081538[more]
alphaTub67C3.463e-15453.05gene:FBgn0087040 transcript:FBtr0076393[more]
CG77941.512e-12745.58gene:FBgn0038565 transcript:FBtr0083540[more]
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BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tuba1c5.596e-16956.68tubulin, alpha 1c [Source:ZFIN;Acc:ZDB-GENE-061114... [more]
tuba1a1.252e-16856.68tubulin, alpha 1a [Source:ZFIN;Acc:ZDB-GENE-090507... [more]
tuba1c1.591e-16856.68tubulin, alpha 1c [Source:ZFIN;Acc:ZDB-GENE-061114... [more]
tuba1b3.354e-16856.45tubulin, alpha 1b [Source:ZFIN;Acc:ZDB-GENE-030822... [more]
tuba1b3.354e-16856.45tubulin, alpha 1b [Source:ZFIN;Acc:ZDB-GENE-030822... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tuba1b1.746e-16856.68tubulin alpha 4a [Source:NCBI gene;Acc:100038108][more]
tuba1b1.746e-16856.68tubulin alpha 4a [Source:NCBI gene;Acc:100038108][more]
tuba1b1.746e-16856.68tubulin alpha 4a [Source:NCBI gene;Acc:100038108][more]
tuba1b1.746e-16856.68tubulin alpha 4a [Source:NCBI gene;Acc:100038108][more]
tuba1b1.843e-16856.68tubulin alpha 4a [Source:NCBI gene;Acc:100038108][more]
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BLAST of Tubulin alpha chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tuba1c9.300e-16956.68tubulin, alpha 1C [Source:MGI Symbol;Acc:MGI:10954... [more]
Tuba1b1.322e-16856.68tubulin, alpha 1B [Source:MGI Symbol;Acc:MGI:10780... [more]
Tuba1a1.396e-16856.68tubulin, alpha 1A [Source:MGI Symbol;Acc:MGI:98869... [more]
Tuba3a1.570e-16855.99tubulin, alpha 3A [Source:MGI Symbol;Acc:MGI:10954... [more]
Tuba3b1.570e-16855.99tubulin, alpha 3B [Source:MGI Symbol;Acc:MGI:10954... [more]
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BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q3ZCJ7|TBA1C_BOVIN5.291e-16856.68Tubulin alpha-1C chain OS=Bos taurus OX=9913 GN=TU... [more]
sp|P68373|TBA1C_MOUSE6.505e-16856.68Tubulin alpha-1C chain OS=Mus musculus OX=10090 GN... [more]
sp|P68365|TBA1C_CRIGR6.505e-16856.68Tubulin alpha-1C chain OS=Cricetulus griseus OX=10... [more]
sp|Q6AYZ1|TBA1C_RAT8.353e-16856.68Tubulin alpha-1C chain OS=Rattus norvegicus OX=101... [more]
sp|Q6P9V9|TBA1B_RAT9.249e-16856.68Tubulin alpha-1B chain OS=Rattus norvegicus OX=101... [more]
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BLAST of Tubulin alpha chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
Q7Z1L88.455e-17357.31Tubulin alpha chain OS=Schmidtea polychroa OX=5005... [more]
A7M6D94.424e-17057.54Tubulin alpha chain OS=Dugesia ryukyuensis OX=7973... [more]
A0A310SSF37.212e-16955.86Tubulin alpha chain (Fragment) OS=Eufriesea mexica... [more]
A0A1X2GXA82.460e-16856.25Tubulin alpha chain OS=Hesseltinella vesiculosa OX... [more]
A0A0L7QLG63.655e-16856.15Tubulin alpha chain (Fragment) OS=Habropoda labori... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000049713.19.941e-16956.68tubulin alpha-1A chain [Source:NCBI gene;Acc:10304... [more]
ENSAMXT00000030346.11.348e-16856.68tubulin alpha-1B chain [Source:NCBI gene;Acc:11119... [more]
si:ch211-114n24.61.029e-16756.22tubulin alpha-1A chain-like [Source:NCBI gene;Acc:... [more]
tuba8l21.168e-16756.22tubulin alpha chain-like [Source:NCBI gene;Acc:103... [more]
tuba1a1.490e-16756.45tubulin alpha chain [Source:NCBI gene;Acc:10303797... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBA3C1.109e-13756.37tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
ENSPMAT00000001139.17.659e-10547.04pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 g... [more]
tubb26.601e-7834.88tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GE... [more]
ENSPMAT00000005307.13.722e-4656.49pep scaffold:Pmarinus_7.0:GL485425:14485:17012:-1 ... [more]
ENSPMAT00000001368.14.501e-4528.41pep scaffold:Pmarinus_7.0:GL476337:866089:875956:-... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
TUB39.309e-15952.89Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB14.492e-15551.96Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB23.736e-9437.12Beta-tubulin; associates with alpha-tubulin (Tub1p... [more]
TUB44.346e-3028.54Gamma-tubulin; involved in nucleating microtubules... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO380671.137e-16856.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO448031.447e-16856.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO448041.495e-16856.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO380911.681e-16856.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO417664.836e-16855.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000037900.12.538e-16956.91tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
ENSORLT00000033163.12.860e-16956.91tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
ENSORLT00000020578.22.860e-16956.91tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
ENSORLT00000020614.21.847e-16856.42tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
tuba1c5.158e-16856.45tubulin alpha-1B chain-like [Source:NCBI gene;Acc:... [more]
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BLAST of Tubulin alpha chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000009421.10.000e+062.65SMESG000009421.1[more]
SMESG000019721.10.000e+060.56SMESG000019721.1[more]
SMESG000057127.15.841e-18059.86SMESG000057127.1[more]
SMESG000057667.11.511e-17659.16SMESG000057667.1[more]
SMESG000010593.11.434e-17558.24SMESG000010593.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035431 ID=SMED30035431|Name=Tubulin alpha chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1851bp
TCTTGTCAATATATAATCTATAATTAATTATAAATATGAAAGAGGTCGTT
TGCATCAACGTAGGGCAGTCGGGGGTCCAGTTGGCCAATGCAGCCTGGGA
ACTATTTTGCATAGAGCATGGGATACAGGCCAACGGCCAGTTGAAGGAAA
GTCTCCAAGATGATGATAGTATAGCATTCAGAACATTTTTCCACGAGGTT
CCAAATGGTCAATATGTACCAAGAGTTATTTCAATGGATTTAGAACCGAC
AGTTTTAGATGAGGTCAGAACTGGGTCATACAGAAACCTTTTCCATCCCG
ATCAGTTGATATCGGGCTGTGAGGATGCTGCAAATAATTTCGCAAGGGGC
CATTTTACACTGGGCAAAGAAATAATCGACCGAATTATGAATGTTGTGCG
GAAATCAGCCGAATGCTGTGACTATTTGCAGAACTTTTTCATTCTCAATA
GTTTTGGGGGAGGAACGGGTTCAGGATTCACATCTTTGCTTCTGGAAAAA
CTGGCTGTTGAGTATTCAAACCGATTTCGTGTTCAATTGAGCATTTGTAT
CAGCCCCGTGTTGACCACCAGCGTGGTGGAGCCGTACAATGCTGTCCTAA
CAATCCACTCATCAATGGAGCATACAGACATTGCCATCCTAACTGACAAT
GAAGCTATTTACGACATATGCCTGGAAACCTTGAACATTCCTAGGCCTAC
CTATGCGAACATCAATAGATTAATTGCTCAAAACTTTAGCGATATGACTG
CTTCAATAAGATTTGAAAGTTCTCTTTCTTCAGACATGGACCAACTTATA
ACGAATCTTATTCCTTATCCTCGAATACACTTTCCCGTGATGTCTCATGC
TCCAAATATTTCAATTAGTCGTGCCGAACACCAACCGTTAGATACCGGCG
AGATCACCAGAATGGTCTTCGGACGCAGCTGCATTTTAGTGACCTGTGAT
CCGACCATTGGCAAATACATGTCTTGTTGTCTCCAATATAGAGGAGATGT
CACGCCTAAAGATGTAAATGCAAGCATTTATGACATAAAAAAGAGGTCTG
ATATTAAATTTGTTGAATGGTGTCCAACTGGTTTCAAACTGGGAATAAAC
TCTCAGCCCAATGTCACAGTTCCAGCATCAGAAATGGCCCAGACATCGAG
GTCCATCTCTATGCTATCGAATACAAGTGCCATTAATCAAGCATGGAAAA
ATGTTAATAAAAAATATGACCTTTTGTTTTATCGGCGAGCATTTGTTCAC
TGGTTTGTTGGCGAAGGCATGGAGGAAGGCGAGATGAATGAAGCAAGAGA
TGACATTGCAATTTTAATAGCAGATTATGAAGAAATTACAGAAGACACTG
AAGAAGTCGGGGAGATTATAACTACTCAACTGGACCAGGGACTAACTGCA
AGATCACAGCAAGCAGAAGCATCGTTTCTACGCCTGAATAAATCTCGAAG
TCAAGAATCTCTGAAACCGTCCATGCAGTTATTTGCTGGGTCGAGAATAT
TGCCAGAAAATCCTAAATCTATAGCTAACAAGACAAATGATTACAAAGCC
GTTTCTGAAGCTGAAGACAACGACGACGAAGACAACGACGAAGAAGACGA
AGACGAAGAGAATGAAGCTAGAATGGAAAACATAAAAGCCACTTCTGGAA
GTGTGTTCACAAGTTCCAAATTGACATCTACGAGTTCTAATTAATTTTAT
TTTCTTATATCTTTCGAATTATTTTTTTCCGTTAATGTTATTGTCGAATT
CTTAGGAAACAAATTCTTTCGCTTTTTGCTGTAAATTTATTTTGCTATTT
TGTTTAAATGATGTATTTATTATTTAATAAATTTTACTTGTTTAAAAAAA
A
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protein sequence of SMED30035431-orf-1

>SMED30035431-orf-1 ID=SMED30035431-orf-1|Name=SMED30035431-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=553bp
MKEVVCINVGQSGVQLANAAWELFCIEHGIQANGQLKESLQDDDSIAFRT
FFHEVPNGQYVPRVISMDLEPTVLDEVRTGSYRNLFHPDQLISGCEDAAN
NFARGHFTLGKEIIDRIMNVVRKSAECCDYLQNFFILNSFGGGTGSGFTS
LLLEKLAVEYSNRFRVQLSICISPVLTTSVVEPYNAVLTIHSSMEHTDIA
ILTDNEAIYDICLETLNIPRPTYANINRLIAQNFSDMTASIRFESSLSSD
MDQLITNLIPYPRIHFPVMSHAPNISISRAEHQPLDTGEITRMVFGRSCI
LVTCDPTIGKYMSCCLQYRGDVTPKDVNASIYDIKKRSDIKFVEWCPTGF
KLGINSQPNVTVPASEMAQTSRSISMLSNTSAINQAWKNVNKKYDLLFYR
RAFVHWFVGEGMEEGEMNEARDDIAILIADYEEITEDTEEVGEIITTQLD
QGLTARSQQAEASFLRLNKSRSQESLKPSMQLFAGSRILPENPKSIANKT
NDYKAVSEAEDNDDEDNDEEDEDEENEARMENIKATSGSVFTSSKLTSTS
SN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0002030early male germ cell
PLANA:0003304testis primordium
Vocabulary: INTERPRO
TermDefinition
IPR002452Alpha_tubulin
IPR000217Tubulin
IPR003008Tubulin_FtsZ_GTPase
IPR018316Tubulin/FtsZ_2-layer-sand-dom
IPR023123Tubulin_C
IPR017975Tubulin_CS
IPR008280Tub_FtsZ_C
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
GO:0007010cytoskeleton organization
Vocabulary: molecular function
TermDefinition
GO:0005525GTP binding
GO:0005200structural constituent of cytoskeleton
GO:0003924GTPase activity
GO:0000166nucleotide binding
Vocabulary: cellular component
TermDefinition
GO:0005874microtubule
GO:0005737cytoplasm
GO:0005856cytoskeleton
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023123Tubulin, C-terminalGENE3DG3DSA:1.10.287.600coord: 1..20
e-value: 2.7E-10
score: 41.6
IPR008280Tubulin/FtsZ, C-terminalSUPERFAMILYSSF55307Tubulin C-terminal domain-likecoord: 1..19