C2 domain

Overview
NameC2 domain
Smed IDSMED30035382
Length (bp)2017
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of C2 domain (SMED30035382) t-SNE clustered cells

Violin plots show distribution of expression levels for C2 domain (SMED30035382) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of C2 domain (SMED30035382) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for C2 domain (SMED30035382) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035382

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30035382h1SMcG0013418 PL05001B1A02smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30035382h1SMcG0013418 dd_Smed_v4_7138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30035382h1SMcG0013418 dd_Smed_v4_7138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30035382h1SMcG0013418 dd_Smed_v4_7138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30035382h1SMcG0013418 dd_Smed_v4_7138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30035382h1SMcG0013418 dd_Smed_v4_7138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30035382h1SMcG0013418 dd_Smed_v4_7138_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of C2 domain vs. TrEMBL
Match: A0A2H1CB78 (C2 domain-containing protein OS=Fasciola hepatica OX=6192 GN=D915_08957 PE=4 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.034e-18
Identity = 54/124 (43.55%), Postives = 81/124 (65.32%), Query Frame = 3 
Query:  216 ICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQXXXXXXXXXXXXYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRS 587
            +  K+KVH +GGK K S+ +V+ + GN QW +    KL  +++DP+VLLV DS+ +H GQV++P+V IP +    N  + P++S  +  ++LEPT K  +P G L YW+WVEEY +EE   K S
Sbjct:   35 LTQKWKVHFVGGKKKMSTSSVLDTYGNPQWDYEVTFKL-ASKSDPVVLLVTDSEDHHVGQVVVPLVTIPPR--PPNVVERPTDSNRLRRADLEPTKKVASPHGMLYYWIWVEEYRSEESDSKSS 155
[Close]
BLAST of C2 domain vs. TrEMBL
Match: A0A4E0R8W6 (C2 domain-containing protein OS=Fasciola hepatica OX=6192 GN=D915_005656 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.470e-17
Identity = 54/124 (43.55%), Postives = 81/124 (65.32%), Query Frame = 3 
Query:  216 ICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQXXXXXXXXXXXXYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRS 587
            +  K+KVH +GGK K S+ +V+ + GN QW +    KL  +++DP+VLLV DS+ +H GQV++P+V IP +    N  + P++S  +  ++LEPT K  +P G L YW+WVEEY +EE   K S
Sbjct:   35 LTQKWKVHFVGGKKKMSTSSVLDTYGNPQWDYEVTFKL-ASKSDPVVLLVTDSEDHHVGQVVVPLVTIPPR--PPNVVERPTDSNRLRRADLEPTKKVASPHGMLYYWIWVEEYRSEESDSKSS 155
[Close]
BLAST of C2 domain vs. TrEMBL
Match: A0A504YE87 (C2 domain OS=Fasciola gigantica OX=46835 GN=FGIG_03532 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 9.448e-17
Identity = 54/124 (43.55%), Postives = 81/124 (65.32%), Query Frame = 3 
Query:  216 ICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQXXXXXXXXXXXXYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRS 587
            +  K+KVH +GGK K S+ +V+ + GN QW +    KL  +++DP+VLLV DS+ +H GQV++P+V IP +    N  + P++S  +  ++LEPT K  +P G L YW+WVEEY +EE   K S
Sbjct:   35 LTQKWKVHFVGGKKKMSTSSVLDTYGNPQWDYEVTFKL-ASKSDPVVLLVTDSEDHHVGQVVVPLVTIPPR--PPNVVERPTDSNRLRRADLEPTKKVASPHGMLYYWIWVEEYRSEESDSKSS 155
[Close]
BLAST of C2 domain vs. TrEMBL
Match: A0A1S8X6E0 (Uncharacterized protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_01876 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.512e-15
Identity = 48/116 (41.38%), Postives = 73/116 (62.93%), Query Frame = 3 
Query:  222 NKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQXXXXXXXXXXXXYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEE 569
             K+KV  LGGK K SS ++++  GN  W +    K+   + +P+ LLV DS+ +H GQV+IP+  +P +    N+S+ P++ + +  ++LEPT K  NP G L YWVWVEEY  E+
Sbjct:   69 QKWKVVFLGGKKKMSSGSILAPAGNPVWDYEVTFKV-AARGEPISLLVTDSEDHHVGQVVIPVTAMPPR--PANSSERPTDPSRLRVADLEPTKKVENPIGTLYYWVWVEEYRAED 181
[Close]
BLAST of C2 domain vs. TrEMBL
Match: A0A5J4NA89 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0008740 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.656e-14
Identity = 46/114 (40.35%), Postives = 69/114 (60.53%), Query Frame = 3 
Query:  216 ICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQXXXXXXXXXXXXYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEY 557
            I  K+KV  LG K    +  V++  GN  W +   +K+   +  P+VLLV DS+ +H GQV+IP   +P +    N+ + P++++ +  S+LEPT K +NP G L YWVWVEEY
Sbjct:   33 ITQKWKVQFLGFKESAHTKPVLAPAGNPVWDYEVTLKV-AVRGGPVVLLVTDSEDHHVGQVVIPASSMPPR--PTNSYERPTDASRLRVSDLEPTKKVSNPLGTLYYWVWVEEY 143
[Close]
BLAST of C2 domain vs. Planmine SMEST
Match: SMESG000068709.1 (SMESG000068709.1)

HSP 1 Score: 827.395 bits (2136), Expect = 0.000e+0
Identity = 515/529 (97.35%), Postives = 515/529 (97.35%), Query Frame = 3 
Query:  117 MLSIPGQKIPRKISIXXXXXXXXXXXXXXXXXXICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQXXXXXXXXXXXXYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNXXXXXXXXXXXXXXXXXTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGXXXXXXXXXDCKITPXXXXXXXXXXXXXXPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTXXXXXXXXXXXXXXXXXXXXXXTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITKVNNDESESIKRENSELKQLNSRLGQQINDLTFELDSVKQTLCKLRLKLLDEGATQFLEI 1703
            MLSIPGQKIPRKISIKVAKAEKLKETKKEGKNKICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQVIIPIVKIPNKPYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNKSMGSLFGSKSKLGSSKTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGKKKSKPSSSDCKITPDSSDVESSSNFSFNPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTNQNLVSSSKNSSKQNNGNLSSSTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITK              ELKQLNSRLGQQINDLTFELDSVKQTLCKLRLKLLDEGATQFLEI
Sbjct:    1 MLSIPGQKIPRKISIKVAKAEKLKETKKEGKNKICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQVIIPIVKIPNKPYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNKSMGSLFGSKSKLGSSKTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGKKKSKPSSSDCKITPDSSDVESSSNFSFNPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTNQNLVSSSKNSSKQNNGNLSSSTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITK--------------ELKQLNSRLGQQINDLTFELDSVKQTLCKLRLKLLDEGATQFLEI 515
[Close]
BLAST of C2 domain vs. Planmine SMEST
Match: SMESG000068709.1 (SMESG000068709.1)

HSP 1 Score: 751.125 bits (1938), Expect = 0.000e+0
Identity = 477/488 (97.75%), Postives = 480/488 (98.36%), Query Frame = 3 
Query:  117 MLSIPGQKIPRKISIXXXXXXXXXXXXXXXXXXICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQXXXXXXXXXXXXYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNXXXXXXXXXXXXXXXXXTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGXXXXXXXXXDCKITPXXXXXXXXXXXXXXPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTXXXXXXXXXXXXXXXXXXXXXXTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITK-VNNDESESIKRENSELK 1577
            MLSIPGQKIPRKISIKVAKAEKLKETKKEGKNKICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQVIIPIVKIPNKPYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNKSMGSLFGSKSKLGSSKTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGKKKSKPSSSDCKITPDSSDVESSSNFSFNPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTNQNLVSSSKNSSKQNNGNLSSSTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITK +  DE    K+EN +LK
Sbjct:    1 MLSIPGQKIPRKISIKVAKAEKLKETKKEGKNKICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQVIIPIVKIPNKPYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNKSMGSLFGSKSKLGSSKTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGKKKSKPSSSDCKITPDSSDVESSSNFSFNPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTNQNLVSSSKNSSKQNNGNLSSSTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITKEIYVDEINQFKKENYQLK 488
[Close]
BLAST of C2 domain vs. Planmine SMEST
Match: SMESG000068709.1 (SMESG000068709.1)

HSP 1 Score: 750.74 bits (1937), Expect = 0.000e+0
Identity = 477/487 (97.95%), Postives = 479/487 (98.36%), Query Frame = 3 
Query:  117 MLSIPGQKIPRKISIXXXXXXXXXXXXXXXXXXICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQXXXXXXXXXXXXYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNXXXXXXXXXXXXXXXXXTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGXXXXXXXXXDCKITPXXXXXXXXXXXXXXPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTXXXXXXXXXXXXXXXXXXXXXXTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITKVNNDESESIKRENSELK 1577
            MLSIPGQKIPRKISIKVAKAEKLKETKKEGKNKICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQVIIPIVKIPNKPYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNKSMGSLFGSKSKLGSSKTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGKKKSKPSSSDCKITPDSSDVESSSNFSFNPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTNQNLVSSSKNSSKQNNGNLSSSTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITK   DE    K+EN +LK
Sbjct:    1 MLSIPGQKIPRKISIKVAKAEKLKETKKEGKNKICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQVIIPIVKIPNKPYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNKSMGSLFGSKSKLGSSKTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGKKKSKPSSSDCKITPDSSDVESSSNFSFNPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTNQNLVSSSKNSSKQNNGNLSSSTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITK---DEINQFKKENYQLK 484
[Close]
BLAST of C2 domain vs. Planmine SMEST
Match: SMESG000068709.1 (SMESG000068709.1)

HSP 1 Score: 745.732 bits (1924), Expect = 0.000e+0
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 3 
Query:  117 MLSIPGQKIPRKISIXXXXXXXXXXXXXXXXXXICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQXXXXXXXXXXXXYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNXXXXXXXXXXXXXXXXXTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGXXXXXXXXXDCKITPXXXXXXXXXXXXXXPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTXXXXXXXXXXXXXXXXXXXXXXTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITK 1526
            MLSIPGQKIPRKISIKVAKAEKLKETKKEGKNKICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQVIIPIVKIPNKPYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNKSMGSLFGSKSKLGSSKTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGKKKSKPSSSDCKITPDSSDVESSSNFSFNPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTNQNLVSSSKNSSKQNNGNLSSSTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITK
Sbjct:    1 MLSIPGQKIPRKISIKVAKAEKLKETKKEGKNKICNKFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQVIIPIVKIPNKPYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGKRSFNKSMGSLFGSKSKLGSSKTNLEIKNRESLGTFGSHSNLNKPEKHKKSSSFLNLKIGGKKKSKPSSSDCKITPDSSDVESSSNFSFNPAHPSNPFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNGNRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMALTANKKVPTEDSVKATNPFGDGMAPTVVPPYTNQNLVSSSKNSSKQNNGNLSSSTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVGSDYSIFSQYSNPFPVQSITK 470
[Close]
BLAST of C2 domain vs. Planmine SMEST
Match: SMESG000061076.1 (SMESG000061076.1)

HSP 1 Score: 66.2402 bits (160), Expect = 4.293e-11
Identity = 48/122 (39.34%), Postives = 73/122 (59.84%), Query Frame = 3 
Query:  225 KFKVHILGGKNKESSPTVMSSNGNVQWLFSCMIKLH-KTQNDPLVLLVEDSDGNHKGQXXXXXXXXXXXXYTYNA--SQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTEEQLGK 581
            K++V  LG   K ++  + S +GN +WL  C I +  +   +P+ LLV DS G+H GQV+IP++ IP KP T      + P   +N+  SELE T K +NP G+L YW+W E+Y+   ++ K
Sbjct:   34 KWRVLFLGVIEKMATAKIESESGNPEWL--CEITVTCRNAFEPISLLVTDSKGHHAGQVVIPLISIPPKPITNGLPNDRPPLLLSNLYKSELEATKKNSNPQGYLYYWMWAEDYWQPGEVQK 153
The following BLAST results are available for this feature:
BLAST of C2 domain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2H1CB781.034e-1843.55C2 domain-containing protein OS=Fasciola hepatica ... [more]
A0A4E0R8W68.470e-1743.55C2 domain-containing protein OS=Fasciola hepatica ... [more]
A0A504YE879.448e-1743.55C2 domain OS=Fasciola gigantica OX=46835 GN=FGIG_0... [more]
A0A1S8X6E02.512e-1541.38Uncharacterized protein (Fragment) OS=Opisthorchis... [more]
A0A5J4NA891.656e-1440.35Uncharacterized protein OS=Paragonimus westermani ... [more]
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BLAST of C2 domain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2 domain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000068709.10.000e+097.35SMESG000068709.1[more]
SMESG000068709.10.000e+097.75SMESG000068709.1[more]
SMESG000068709.10.000e+097.95SMESG000068709.1[more]
SMESG000068709.10.000e+0100.00SMESG000068709.1[more]
SMESG000061076.14.293e-1139.34SMESG000061076.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035382 ID=SMED30035382|Name=C2 domain|organism=Schmidtea mediterranea sexual|type=transcript|length=2017bp
ATTTAGACATAACCATAATTTTATTTTATGCTTTCTGTATTATTTTAGAT
AAATTTCTATTTTTCATATTTATACAAGTTATTGGTATTTTAAATTGTTT
ATATTATTAAATCGATATGCTTTCAATTCCCGGACAAAAGATACCTCGTA
AAATATCAATTAAAGTTGCTAAAGCCGAAAAGCTTAAAGAGACTAAAAAG
GAAGGCAAAAATAAAATATGTAATAAATTTAAAGTTCATATACTCGGTGG
AAAGAATAAAGAATCGAGTCCAACTGTAATGAGCTCAAATGGAAATGTTC
AATGGCTTTTTTCTTGCATGATAAAACTTCATAAAACTCAGAATGATCCA
TTAGTTCTTTTAGTTGAAGATTCTGATGGTAATCACAAAGGACAAGTTAT
AATTCCAATTGTCAAAATTCCTAATAAACCATACACGTATAATGCAAGTC
AAGAACCTTCAAATTCAACTAACATTCTCTGTTCTGAATTAGAACCTACT
AATAAATGTAATAACCCATCCGGATTTTTACTTTATTGGGTATGGGTCGA
AGAATATTTTACTGAAGAACAATTAGGCAAAAGATCATTCAACAAGAGCA
TGGGGTCGTTATTTGGATCGAAAAGCAAACTTGGTTCATCCAAAACAAAT
CTTGAAATAAAAAATCGCGAGTCACTAGGCACTTTCGGTTCTCATAGTAA
TCTCAACAAACCAGAAAAACATAAAAAGAGCTCCAGTTTTTTGAATTTAA
AAATCGGTGGGAAGAAAAAATCTAAGCCTAGCTCATCAGACTGCAAAATT
ACTCCTGATTCGAGTGATGTCGAATCCTCGTCGAATTTCAGCTTTAATCC
AGCTCATCCATCTAATCCGTTTGAAAATGATCCTGATTTGGTGAAAACTG
ATAGAGTATCTATCAAAAATGGTTCTTTGGCAGAAGATGACAGTTTATCA
AGGAAATTCAATAAGCATTCTAACGATTCTTATAATTTTAACAAGTCAGA
CATAACTTATAATGGAAACCGTGATAACCTTTCAGAAGCTCAATTCAGTA
TAAAATCCGGTGATAAAAACATACTGTCAGCCTTTGAATCAGTCTCAAAG
GTAGAAAAAGCTTCACGTCAATCAATTGAGGAAATAAACAATGGTAAAAC
ACTTTTAATGATGGCCCTAACGGCTAATAAGAAAGTTCCAACTGAAGACA
GTGTCAAAGCTACGAATCCTTTTGGTGATGGCATGGCACCAACGGTGGTA
CCGCCATACACTAATCAGAATCTTGTTTCGAGCAGCAAAAATAGTAGCAA
ACAGAATAATGGAAATTTAAGTTCTTCGACCTTACCGAACAAATATAACA
CAATGAAAGATTTACAGACCTACAAAGAGCCACCATTTAATATTGATTCA
TCTATCGTATTTTCCAATGAAACAGAATCAACTTATCGGCCTGGCCATAA
TAAATCGGGTGTAGGAAGTGATTATTCAATATTCAGTCAATATAGTAATC
CATTTCCAGTACAGAGTATTACAAAAGTCAACAATGATGAATCTGAGAGT
ATTAAGAGGGAAAACTCAGAATTGAAGCAGTTGAATAGTAGATTGGGGCA
GCAAATCAATGATTTGACATTTGAACTCGACTCAGTCAAGCAGACTTTGT
GCAAGTTAAGACTTAAATTATTAGATGAAGGTGCTACACAATTTTTGGAA
ATCTAGAAGAATTATATTTCAAGAAATTATTTATTTATTCTTTCATTTCC
ATGTTGATGAAAATATCTATTTTATATTTTATAACTTTTGTTAAATGTGT
TATCAAGATAATTTTATTATTGACACTTTTGTTACACTGCTGTATTATTG
TGATTGGTGATTTTTTGTTTTTTCGTTTGTTGATCAGTGACGAACTTGCT
CAATTTATAATACAATACAACATTTATGATAAATTGATTGTTATTCATTG
TCTTGTGCGGTTTACACTTGTTTACTTTCAATTTAATTAATAAATCATTG
CTTTTGTGAAAAAAAAA
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protein sequence of SMED30035382-orf-1

>SMED30035382-orf-1 ID=SMED30035382-orf-1|Name=SMED30035382-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=530bp
MLSIPGQKIPRKISIKVAKAEKLKETKKEGKNKICNKFKVHILGGKNKES
SPTVMSSNGNVQWLFSCMIKLHKTQNDPLVLLVEDSDGNHKGQVIIPIVK
IPNKPYTYNASQEPSNSTNILCSELEPTNKCNNPSGFLLYWVWVEEYFTE
EQLGKRSFNKSMGSLFGSKSKLGSSKTNLEIKNRESLGTFGSHSNLNKPE
KHKKSSSFLNLKIGGKKKSKPSSSDCKITPDSSDVESSSNFSFNPAHPSN
PFENDPDLVKTDRVSIKNGSLAEDDSLSRKFNKHSNDSYNFNKSDITYNG
NRDNLSEAQFSIKSGDKNILSAFESVSKVEKASRQSIEEINNGKTLLMMA
LTANKKVPTEDSVKATNPFGDGMAPTVVPPYTNQNLVSSSKNSSKQNNGN
LSSSTLPNKYNTMKDLQTYKEPPFNIDSSIVFSNETESTYRPGHNKSGVG
SDYSIFSQYSNPFPVQSITKVNNDESESIKRENSELKQLNSRLGQQINDL
TFELDSVKQTLCKLRLKLLDEGATQFLEI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR035892C2_domain_sf
IPR000008C2_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 472..499
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 184..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 225..249
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 387..409
IPR035892C2 domain superfamilyGENE3DG3DSA:2.60.40.150coord: 8..124
e-value: 4.0E-5
score: 25.3