tRNA (guanine(37)-N1)-methyltransferase

Overview


Name	tRNA (guanine(37)-N1)-methyltransferase
Smed ID	SMED30035364
Length (bp)	1401

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of tRNA (guanine(37)-N1)-methyltransferase (SMED30035364) t-SNE clustered cells

Violin plots show distribution of expression levels for tRNA (guanine(37)-N1)-methyltransferase (SMED30035364) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of tRNA (guanine(37)-N1)-methyltransferase (SMED30035364) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for tRNA (guanine(37)-N1)-methyltransferase (SMED30035364) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30035364

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
neoblast	SMED30035364	SMESG000019159.1	dd_Smed_v4_7597_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

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Homology

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Human
Match: TRMT5 (tRNA methyltransferase 5 [Source:HGNC Symbol;Acc:HGNC:23141])

HSP 1 Score: 239.58 bits (610), Expect = 9.472e-73
Identity = 145/417 (34.77%), Postives = 239/417 (57.31%), Query Frame = 1

Query:   76 LWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKS-EKLPKFHLIMNLPSIAVDFLSAI--VANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLR---ERVKSVNLRFVRNVSPSKDMFCAEIIVNLSAISGN 1305
            L+  P +     K+++ A  + ++ P+LKV+      +M+  K+ +       +  +     E + I+LDP K+ + H+     E  +L+QL++      I    + + Y+++K   +   +LP+ Q +T GF+ IGHI HLNL++   P K++IG++++DK  G+T+ V+K+N ID  +R F +E+L+GE+  +T ++EN + ++FDFSKVYWN RLSTEH R+ + L+  DV++D   GVGPFAIP AKK C V ANDLN ES KW   N ++N    +   + + NLD  DF++  +   +++++  S E+ P  H++MNLP+ A++FLSA   + + Q      S+    V CY F     P E + ++    L    E   SV+L  VRNV+P+K+M C    +  S +  N
Sbjct:   72 LFSPPSDVRGMTKLDRTAFKKTVNIPVLKVRKEIVSKLMRSLKRAALQRPGIRRVIEDPEDKESRLIMLDPYKIFT-HDSFEKAELSVLEQLNVSPQ---ISKYNLELTYEHFKSEEILRAVLPEGQDVTSGFSRIGHIAHLNLRDHQLPFKHLIGQVMIDKNPGITSAVNKINNIDNMYRNFQMEVLSGEQNMMTKVRENNYTYEFDFSKVYWNPRLSTEHSRITELLKPGDVLFDVFAGVGPFAIPVAKKNCTVFANDLNPESHKWLLYNCKLNKVDQK---VKVFNLDGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFKWLLDGQ---PCSSEFLPIVHCYSFSKDANPAEDVRQRAGAVLGISLEACSSVHL--VRNVAPNKEMLCITFQIPASVLYKN 476

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Celegans
Match: C53A5.17 (tRNA (guanine(37)-N1)-methyltransferase [Source:UniProtKB/Swiss-Prot;Acc:A8WHT1])

HSP 1 Score: 203.371 bits (516), Expect = 8.936e-60
Identity = 147/438 (33.56%), Postives = 226/438 (51.60%), Query Frame = 1

Query:   61 SNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFD-KSKKFDGTSEYKG---IILDPSKLNSIHNELSDKESVILKQLDIEF-NDHIIEFMKI--NVVYDNYKLSTLFDWILPDNQHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAA--KKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXV-----------DKSEKLPKFHLIMNLPSIAVDFLSAIVA-----NDQIEFITRSDVF-LHVKCYCFXXXXXXXXXXXXXXNTQLR-------ERVKSVNLRFVRNVSPSKDMFCAEI 1275
            SN S L + P+      K+ K+A    +DFP+++++  +   V +  +   P LI    K  K    SE  G   ++  P K+             IL+ L  E  N+ ++++  +  ++ ++N+   ++F  +LP     + +T  GHI+H N  +E  P ++II E++LDKV+   TVV K N I   +R   LELLAGE+ YVT +KE G +FK DFSKVYWNSRLS EH RV        +VYDAC G+GPF +PA   +K  +VVANDLN ES KW K N+ +N  K     I++HN+DA  FI++ +   ++ ++           +  + + + H++MNLP+ AV+FL A         D+IE +     +  +V CY F    +       +   +         ER   V    VR VS  K+MFCA++
Sbjct:    9 SNMSIL-QPPEVVRGMTKLEKEAFRMPVDFPVIEIEARDAGIVGRRVR-LEPYLIGHKLKPLKNLVDSEKDGKKYLVFHPDKVQEDETRFK-----ILELLKRELGNEKLLDWTTLSKDLTFENWDAKSIFKAVLPVGIDYSSYTQTGHIIHCNFADEILPFRFIIAEVLLDKVNNCKTVVQKGNIITNVYRNLDLELLAGEDNYVTEVKETGLRFKMDFSKVYWNSRLSHEHERVSGMFNTQSLVYDACCGIGPFVLPATLKRKPKRVVANDLNPESVKWLKVNVGLNKIKEER--IEIHNMDAKMFIKENVADDVVRLMLEESTAGEFENEVPKPMSEVHVVMNLPAYAVNFLPAFRGALSRFKDEIEKVPLDKRYKWNVYCYLFAKSHVDVPDSWYEDEARRMCDEKTKWERSLVVKCHNVRTVSSRKEMFCAQL 437

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Fly
Match: CG32281 (gene:FBgn0052281 transcript:FBtr0073034)

HSP 1 Score: 223.016 bits (567), Expect = 3.414e-67
Identity = 140/412 (33.98%), Postives = 229/412 (55.58%), Query Frame = 1

Query:  142 IDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKL---NSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILP-DNQHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLP-IDLHNLDAGDFXXXXXXXXXXXXVDKSEKLP-KFHLIMNLPSIAVDFLSA---IVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKS---VNLRFVRNVSPSKDMFCAEIIVNLSAISGNFEAESKRRKLDE 1341
            +  P L+V  +  + VM + KKF   L+K +         + + I+L P+ +   +S+  E   ++ V         N     F  + + Y+N+  + +   +LP + + LT ++ IGHI HLNL++   P K +IG+++ DK+    TVV+K + ID  +R F LEL+ G+  Y    KENG  F+FDFSKVYWN RLSTEH R++  L+ DDV+YD   GVGPF+IPAAKK C V+ANDLN ES +W    ++ N+K+++ LP I + N D   FI + +   +++ +  ++      H+ MNLP++AV+FL A   + + D++  +  +  +  V  Y F   E  KE + + + + L   +       + FVRNV+P+KDM+     ++L+ ++   E E  R++  E
Sbjct:   50 VQVPRLRVPESQVQRVMPLVKKF---LLKMEHLHPVRAVDQSREILLHPTPVKNWDSLPTEDLQRQKV---------NAENFSFADLELRYENWSANEILKSVLPTEEEGLTSYSRIGHIAHLNLRDHLLPYKQLIGQVLRDKLPNCRTVVNKASSIDNTYRNFQLELICGDPDYQVETKENGVPFEFDFSKVYWNPRLSTEHERIVKMLKSDDVLYDVFAGVGPFSIPAAKKRCHVLANDLNPESFRW----LQHNAKRNKCLPNIKMSNKDGRQFIVEELREDLLKRLCTTDTTTYGIHITMNLPAMAVEFLDAFRGLYSADELAQLPTNVCYPTVHVYSFAKGENTKELVRQLVESNLGASLDENLLQGINFVRNVAPNKDMYRVSFKLSLNMLTTLKEVEVTRKRYAE 445

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Zebrafish
Match: trmt5 (tRNA methyltransferase 5 [Source:NCBI gene;Acc:564078])

HSP 1 Score: 245.358 bits (625), Expect = 1.696e-75
Identity = 146/428 (34.11%), Postives = 240/428 (56.07%), Query Frame = 1

Query:   22 NFNYLMLSC-TMSQSNQS--ALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFS---PDLIKFDKSKKFDGTS-----EYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKS-VNLRFVRNVSPSKDMFC 1266
            N N+ M S   M+  +Q+   L++ P       ++++ A  + +  P +++  N     +K  KK +   P L +  +    DG +     E++ ++LDP+ + S  +  SD E+  LK   +      I+  ++ + Y+N+K   +   +LP+ Q +T GF+ +GHI H+NL+E   P + +IG++I+DK  G+T VV+K N ID+ +R F +E+LAGE   V  ++ENG  ++FDFS+VYWN RLSTEH R++  L + D V D   GVGPFAIPAA++GC V+ANDLN ES +W + N ++N    +   I   NLD  DFI   +   +  ++  S+K+   H++MNLP++A++FL A       E          V CY F  +  P+  + E+    L+  ++   ++  VRNV+P+K+M C
Sbjct:   27 NLNHFMRSQKVMTDQSQTDLGLFKPPSTVRGMTELDRSAFNQTVSVPAIRLPTNILNKAVKSLKKVALQRPGLKRVVEEHSEDGNADCGNIEHRLLLLDPNSITSSDSFGSD-EAEALKAYSVP---QEIQSYELKLTYENFKSEEILRAVLPEGQGVTSGFSRVGHIAHMNLREHQLPYRKLIGQVIIDKNPGITCVVNKTNTIDSTYRNFQMEVLAGESNMVAKVRENGVLYEFDFSRVYWNPRLSTEHERIVSLLHRGDTVVDVFAGVGPFAIPAARRGCAVLANDLNPESFRWLQHNAKLNKVDQK---ITTSNLDGRDFIRGPVRERLPALMKGSQKI---HVVMNLPALALEFLDAFKGLLDPEPDQSLSNLPQVHCYGFSKENDPQRDVVERAEASLKTNLQGQCSVHLVRNVAPNKEMMC 444

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Mouse
Match: Trmt5 (TRM5 tRNA methyltransferase 5 [Source:MGI Symbol;Acc:MGI:1923607])

HSP 1 Score: 232.646 bits (592), Expect = 2.663e-70
Identity = 132/402 (32.84%), Postives = 232/402 (57.71%), Query Frame = 1

Query:   70 SALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKS-EKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVK-SVNLRFVRNVSPSKDMFC 1266
            S L+  P +     ++++ A  + +  P+LK +      +M+  ++ +       +  +     + + I+LDP ++ +  +     E  +LK+LD+  +  + ++  + + Y+N+K   +   +LP+ Q +T GF+ +GHI HLNL++   P K++IG++++DK  G+T+ V+K + ID  +R F +E+L GEE  +T ++EN + ++FDFSKVYWN RLSTEH R+ + L   DV++D   GVGPFAIPAA+K C V ANDLN ES KW   N ++N    +   + + N+D  DFI+  +   ++  +  S E  P  H++MNLP+ A++FLS   +    +  + +++   V CYCF     P + + ++    L   ++ S ++  VRNV+P+K+M C
Sbjct:   63 SELFSPPSDVRGMRELDRTAFKKTVSIPVLKARKEVVNRLMRALRRVALQRPGIKRVIEDPKDEDSRLIMLDPYRMLTA-DSFDKAELGVLKELDV--SPQLSQY-NLELTYENFKSEEILKAVLPEGQDVTSGFSRVGHIAHLNLRDHQLPFKHLIGQVMVDKNPGITSAVNKTSNIDNTYRNFQMEVLCGEENMLTKVRENNYTYEFDFSKVYWNPRLSTEHGRITELLNPGDVLFDVFAGVGPFAIPAARKNCTVFANDLNPESHKWLLHNCKLNKVDQK---VKVFNMDGKDFIQGPVREELMLRLGLSAEAKPSVHIVMNLPAKAIEFLSVFRSLLDGQPCS-TELLPTVHCYCFSKDSDPAKDVRQQAEAVLGVSLETSSSVHLVRNVAPNKEMLC 456

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. UniProt/SwissProt
Match: sp|F6VSS6|TRM5_XENTR (tRNA (guanine(37)-N1)-methyltransferase OS=Xenopus tropicalis OX=8364 GN=trmt5 PE=3 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 9.018e-77
Identity = 155/419 (36.99%), Postives = 240/419 (57.28%), Query Frame = 1

Query:  115 VNKQAACREIDFPILKVKINNFKDVMKIFKKF---SPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVD--KSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKSVNLRFVRNVSPSKDMFCAEIIVNLSA----ISGNFEAESKRRKLDE 1341
            +N+ A  + I  P++K+K      +MK  K      P L +  +  K D     K ++LDP K+ SI +   + + V+ KQ D+  N  + ++ ++ + Y+N+K   +   +LP  Q +T GF+ +GHI H+NL++   P K +IG++ILDK  G+T+VV+K N ID+ +R F +E+LAGEE  +T +KEN   ++FDFSKVYWN RL TEH R+I  L+  DV++D   GVGPFA+PAAKK C V ANDLN ES KW   N ++N  + R   +   N D  DFI+  +   +++  D   +E+ P  H+ MNLP++AV+FL A   N   E    S +   + CY F   + P + ++ +  + L   ++  ++  VRNV+P+K+M C    +  S     +S   E ESKR +  E
Sbjct:   74 LNRDAFDKTIHVPVIKIKKEIINRLMKSLKHRLIQRPSLKRVIEDPKDDVN---KLVLLDPYKVKSI-DSFDESDHVLFKQFDV--NPQVSQY-ELQLTYENFKCEEILRAVLPKGQDVTSGFSRVGHIAHMNLRDHQLPYKNVIGQVILDKNPGITSVVNKTNTIDSTYRNFQMEVLAGEENMITKVKENYVTYEFDFSKVYWNPRLGTEHNRIIGFLKARDVLFDVFAGVGPFAVPAAKKNCTVYANDLNPESYKWLLHNCKLNKVEKR---VQAFNTDGRDFIKTTIKKELLKYADMPSAEEKPSLHIAMNLPALAVEFLDAF-KNLLEEEPCNSFILPTIHCYSFSKDDDPLQDVKARAESFLGTTLEDCSMHLVRNVAPNKEMVCISFKLPSSVLFQRLSDTGEPESKRPRTAE 481

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. UniProt/SwissProt
Match: sp|B8A5G9|TRM5_DANRE (tRNA (guanine(37)-N1)-methyltransferase OS=Danio rerio OX=7955 GN=trmt5 PE=3 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 1.280e-74
Identity = 146/428 (34.11%), Postives = 240/428 (56.07%), Query Frame = 1

Query:   22 NFNYLMLSC-TMSQSNQS--ALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFS---PDLIKFDKSKKFDGTS-----EYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKS-VNLRFVRNVSPSKDMFC 1266
            N N+ M S   M+  +Q+   L++ P       ++++ A  + +  P +++  N     +K  KK +   P L +  +    DG +     E++ ++LDP+ + S  +  SD E+  LK   +      I+  ++ + Y+N+K   +   +LP+ Q +T GF+ +GHI H+NL+E   P + +IG++I+DK  G+T VV+K N ID+ +R F +E+LAGE   V  ++ENG  ++FDFS+VYWN RLSTEH R++  L + D V D   GVGPFAIPAA++GC V+ANDLN ES +W + N ++N    +   I   NLD  DFI   +   +  ++  S+K+   H++MNLP++A++FL A       E          V CY F  +  P+  + E+    L+  ++   ++  VRNV+P+K+M C
Sbjct:   27 NLNHFMRSQKVMTDQSQTDLGLFKPPSTVRGMTELDRSAFNQTVSVPAIRLPTNILNKAVKSLKKVALQRPGLKRVVEEHSEDGNADCGNIEHRLLLLDPNSITSSDSFGSD-EAEALKAYSVP---QEIQSYELKLTYENFKSEEILRAVLPEGQGVTSGFSRVGHIAHMNLREHQLPYRKLIGQVIIDKNPGITCVVNKTNTIDSTYRNFQMEVLAGESNMVAKVRENGVLYEFDFSRVYWNPRLSTEHERIVSLLHRGDTVVDVFAGVGPFAIPAARRGCAVLANDLNPESFRWLQHNAKLNKVDQK---ITTSNLDGRDFIRGPVRERLPALMKGSQKI---HVVMNLPALALEFLDAFKGLLDPEPDQSLSNLPQVHCYGFSKENDPQRDVVERAEASLKTNLQGQCSVHLVRNVAPNKEMMC 444

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. UniProt/SwissProt
Match: sp|Q3MHN8|TRM5_BOVIN (tRNA (guanine(37)-N1)-methyltransferase OS=Bos taurus OX=9913 GN=TRMT5 PE=2 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 1.126e-73
Identity = 145/405 (35.80%), Postives = 240/405 (59.26%), Query Frame = 1

Query:   70 SALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVD--KSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLR---ERVKSVNLRFVRNVSPSKDMFC 1266
            S L+  P       ++++ A  + +  P+LKV+      +M+  K+ +       +  +     E + I+LDP K+ ++ +    +E  ILKQL++  N  I ++  +++ Y+N+K   +   +LP+ Q +T GF+ +GHI HLNL++   P K++IG++++DK  G+T+ V+K+N ID  +R F +E+L+GEE  +T ++EN + ++FDFSKVYWN RLSTEH R+ + L+  DV++D   GVGPFAIPAAKK C V ANDLN ES KW   N ++N    +   + + NLD  DF++  +   +++ +     E+    H++MNLP+ A++FLSA  A  + +    S++   V CY F     P + ++++  T L    E   SV+L  VRNV+P+K+M C
Sbjct:   68 SELFSPPSEVRGMTELDRTAFKKTVTIPVLKVRKEVVNKLMRSLKRAALQRPGIKRVIEDPEDGEGRLIMLDPYKMFTV-DSFEKEELSILKQLNV--NPQISKY-NLDLTYENFKSEEILRAVLPEGQDVTSGFSRVGHIAHLNLRDHQLPYKHLIGQVMIDKNPGITSAVNKINNIDNTYRNFEMEVLSGEENMMTKVRENNYTYEFDFSKVYWNPRLSTEHSRITELLKPGDVLFDVFAGVGPFAIPAAKKKCTVFANDLNPESHKWLLHNCKLNKVDQK---VKVFNLDGRDFLQGPVREELMQQLGPLSKERKHSVHIVMNLPAKAIEFLSAFKALLEGQPCG-SELLPIVHCYSFSKDANPAKDVQQRAATVLGISLEAYSSVHL--VRNVAPNKEMLC 462

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. UniProt/SwissProt
Match: sp|F7GSQ4|TRM5_MACMU (tRNA (guanine(37)-N1)-methyltransferase OS=Macaca mulatta OX=9544 GN=TRMT5 PE=3 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 8.494e-72
Identity = 149/430 (34.65%), Postives = 243/430 (56.51%), Query Frame = 1

Query:    7 SPFLFNFNYLMLSCTM----SQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKS-EKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLH-VKCYCFXXXXXXXXXXXXXXNTQLR---ERVKSVNLRFVRNVSPSKDMFC 1266
            SP +F         TM    +    S L+  P +     K+++ A  + ++ P+LKV+      +M+  ++ +       +  +     E + I+LDP K+ + H+     E  +L+QL++      I    + + Y+N+K   +   +LP+ Q +T GF+ +GHI HLNL++   P K +IG++++DK  G+T+ V+K+N ID  +R F +E+L+GE+  +T ++EN + ++FDFSKVYWN RLSTEH R+ + L+  DV++D   GVGPFAIP AKK C V ANDLN ES KW   N ++N    +   + + NLD  DF++  +   +I+++  S E+ P  H++MNLP+ A++FLSA      ++    S+ FL  V CY F     P + + ++    L    E   SV+L  VRNV+P+K+M C
Sbjct:   45 SPGIFLLGQRKRFSTMPEIETHERDSELFSPPSDVRGMTKLDRTAFKKTVNIPVLKVRKEIVSRLMRSLRRAALQRPGIKRVIEDPEDKESRLILLDPYKIFT-HDSFEKAELSVLEQLNVSPQ---ISKYNLELTYENFKSEEILRAVLPEGQDVTSGFSRVGHIAHLNLRDHQLPFKQLIGQVMIDKNPGITSAVNKINNIDNMYRNFHMEVLSGEQNMMTKVRENKYTYEFDFSKVYWNPRLSTEHSRITELLKAGDVLFDVFAGVGPFAIPVAKKNCTVFANDLNPESHKWLLHNCKLNKVDQK---VKIFNLDGKDFLQGPVKEELIQLLSLSKERKPSVHIVMNLPAKAIEFLSAF--KWLLDGQPCSNEFLPIVHCYSFSKDANPAKDVRQRAGAVLGISLEACSSVHL--VRNVAPNKEMLC 463

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. UniProt/SwissProt
Match: sp|Q95KJ2|TRM5_MACFA (tRNA (guanine(37)-N1)-methyltransferase OS=Macaca fascicularis OX=9541 GN=TRMT5 PE=2 SV=1)

HSP 1 Score: 236.498 bits (602), Expect = 7.589e-71
Identity = 148/430 (34.42%), Postives = 242/430 (56.28%), Query Frame = 1

Query:    7 SPFLFNFNYLMLSCTM----SQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKS-EKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLH-VKCYCFXXXXXXXXXXXXXXNTQLR---ERVKSVNLRFVRNVSPSKDMFC 1266
            SP +F         TM    +    S L+  P +     K+++ A  + ++ P+LKV+      +M+  ++ +       +  +     E + I+LDP K+ + H+        +L+QL++      I    + + Y+N+K   +   +LP+ Q +T GF+ +GHI HLNL++   P K +IG++++DK  G+T+ V+K+N ID  +R F +E+L+GE+  +T ++EN + ++FDFSKVYWN RLSTEH R+ + L+  DV++D   GVGPFAIP AKK C V ANDLN ES KW   N ++N    +   + + NLD  DF++  +   +I+++  S E+ P  H++MNLP+ A++FLSA      ++    S+ FL  V CY F     P + + ++    L    E   SV+L  VRNV+P+K+M C
Sbjct:   45 SPGIFLLGQRKRFSTMPEIETHEGDSELFSPPSDVRGMTKLDRTAFKKTVNIPVLKVRKEIVSRLMRSLRRAALQRPGIKRVIEDPEDKESRLILLDPYKIFT-HDSFEKAGLSVLEQLNVSPQ---ISKYNLELTYENFKSEEILRAVLPEGQDVTSGFSRVGHIAHLNLRDHQLPFKQLIGQVMIDKNPGITSAVNKINNIDNMYRNFHMEVLSGEQNMMTKVRENKYTYEFDFSKVYWNPRLSTEHSRITELLKAGDVLFDVFAGVGPFAIPVAKKNCTVFANDLNPESHKWLLHNCKLNKVDQK---VKIFNLDGKDFLQGPVKEELIQLLSLSKERKPSVHIVMNLPAKAIEFLSAF--KWLLDGQPCSNEFLPIVHCYSFSKDANPAKDVRQRAGAVLGISLEACSSVHL--VRNVAPNKEMLC 463

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. TrEMBL
Match: A0A4W3GUF6 (tRNA (guanine(37)-N1)-methyltransferase OS=Callorhinchus milii OX=7868 GN=TRMT5 PE=3 SV=1)

HSP 1 Score: 253.447 bits (646), Expect = 4.545e-75
Identity = 162/437 (37.07%), Postives = 247/437 (56.52%), Query Frame = 1

Query:   76 LWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFD-GTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXX--XXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAI--VANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKS-VNLRFVRNVSPSKDMFC------AEIIVN-LSAISGNFEAES-KRRKLDE 1341
            L+  P       ++NK +  +++  P LKVK +    VMK  K  +  L +    +  D G   YK I+LDP K++S  +     + +ILK  +I+   H  +   + + Y+N+K   +   +LP+ Q +T GF+ +GHIVH+NL++   P KY+IG++I+DK  G+T+VV+K + ID  +R F +E+LAGE+  +  +KEN   ++FDFSKVYWN RL TEH R+++ L+  DVVYD   GVGPFAIPAAKK CKV+ANDLN +S KW   N ++N        +++ NLD  +FI   +         V  S+     H+IMNLP +AV+FL A   +  DQ       ++   V CY F   + P   I+++    L   ++   ++  VRNVSP+K+M C      AE++ N   A+  + E  + KR +LDE
Sbjct:   55 LYAAPATVRGMTQLNKNSFRKKLTLPALKVKTDEINKVMKSLKNVA--LKRPGLKRVVDLGDEAYKLILLDPFKISS-SDSFDASQKMILKNYNIDAQIHRYD---LTMTYENFKSEEILKAVLPEGQDVTSGFSRVGHIVHMNLRDHQLPYKYLIGQVIMDKNPGITSVVNKTSMIDNAYRNFQMEVLAGEDNMIAKVKENNITYEFDFSKVYWNPRLGTEHDRIVNLLKPGDVVYDVFAGVGPFAIPAAKKKCKVIANDLNPDSYKWLVHNCKVNKIDKN---VEIFNLDGREFILGPLKDNLVKQYEVLSSKDTTSIHVIMNLPGMAVEFLDAFRRLLKDQ----NPVNILPTVHCYSFSKSDKPALDIQQRAENFLGASLEGHCSIHLVRNVSPNKEMMCISFKIPAEVLYNDCPAMEDSAEEPAPKRLRLDE 478

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. TrEMBL
Match: V9KPY0 (tRNA (guanine(37)-N1)-methyltransferase OS=Callorhinchus milii OX=7868 GN=TRMT5 PE=2 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 8.866e-75
Identity = 162/437 (37.07%), Postives = 247/437 (56.52%), Query Frame = 1

Query:   76 LWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFD-GTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXX--XXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAI--VANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKS-VNLRFVRNVSPSKDMFC------AEIIVN-LSAISGNFEAES-KRRKLDE 1341
            L+  P       ++NK +  +++  P LKVK +    VMK  K  +  L +    +  D G   YK I+LDP K++S  +     + +ILK  +I+   H  +   + + Y+N+K   +   +LP+ Q +T GF+ +GHIVH+NL++   P KY+IG++I+DK  G+T+VV+K + ID  +R F +E+LAGE+  +  +KEN   ++FDFSKVYWN RL TEH R+++ L+  DVVYD   GVGPFAIPAAKK CKV+ANDLN +S KW   N ++N        +++ NLD  +FI   +         V  S+     H+IMNLP +AV+FL A   +  DQ       ++   V CY F   + P   I+++    L   ++   ++  VRNVSP+K+M C      AE++ N   A+  + E  + KR +LDE
Sbjct:   66 LYAAPATVRGMTQLNKNSFRKKLTLPALKVKTDEINKVMKSLKNVA--LKRPGLKRVVDLGDEAYKLILLDPFKISS-SDSFDASQKMILKNYNIDAQIHRYD---LTMTYENFKSEEILKAVLPEGQDVTSGFSRVGHIVHMNLRDHQLPYKYLIGQVIMDKNPGITSVVNKTSMIDNAYRNFQMEVLAGEDNMIAKVKENNITYEFDFSKVYWNPRLGTEHDRIVNLLKPGDVVYDVFAGVGPFAIPAAKKKCKVIANDLNPDSYKWLVHNCKVNKIDKN---VEIFNLDGREFILGPLKDNLVKQYEVLSSKDTTSIHVIMNLPGMAVEFLDAFRRLLKDQ----NPVNILPTVHCYSFSKSDKPALDIQQRAENFLGASLEGHCSIHLVRNVSPNKEMMCISFKIPAEVLYNDCPAMEDSAEEPAPKRLRLDE 489

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. TrEMBL
Match: A0A4W3GR83 (tRNA (guanine(37)-N1)-methyltransferase OS=Callorhinchus milii OX=7868 GN=TRMT5 PE=3 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.035e-74
Identity = 162/437 (37.07%), Postives = 247/437 (56.52%), Query Frame = 1

Query:   76 LWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFD-GTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXX--XXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAI--VANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKS-VNLRFVRNVSPSKDMFC------AEIIVN-LSAISGNFEAES-KRRKLDE 1341
            L+  P       ++NK +  +++  P LKVK +    VMK  K  +  L +    +  D G   YK I+LDP K++S  +     + +ILK  +I+   H  +   + + Y+N+K   +   +LP+ Q +T GF+ +GHIVH+NL++   P KY+IG++I+DK  G+T+VV+K + ID  +R F +E+LAGE+  +  +KEN   ++FDFSKVYWN RL TEH R+++ L+  DVVYD   GVGPFAIPAAKK CKV+ANDLN +S KW   N ++N        +++ NLD  +FI   +         V  S+     H+IMNLP +AV+FL A   +  DQ       ++   V CY F   + P   I+++    L   ++   ++  VRNVSP+K+M C      AE++ N   A+  + E  + KR +LDE
Sbjct:   65 LYAAPATVRGMTQLNKNSFRKKLTLPALKVKTDEINKVMKSLKNVA--LKRPGLKRVVDLGDEAYKLILLDPFKISS-SDSFDASQKMILKNYNIDAQIHRYD---LTMTYENFKSEEILKAVLPEGQDVTSGFSRVGHIVHMNLRDHQLPYKYLIGQVIMDKNPGITSVVNKTSMIDNAYRNFQMEVLAGEDNMIAKVKENNITYEFDFSKVYWNPRLGTEHDRIVNLLKPGDVVYDVFAGVGPFAIPAAKKKCKVIANDLNPDSYKWLVHNCKVNKIDKN---VEIFNLDGREFILGPLKDNLVKQYEVLSSKDTTSIHVIMNLPGMAVEFLDAFRRLLKDQ----NPVNILPTVHCYSFSKSDKPALDIQQRAENFLGASLEGHCSIHLVRNVSPNKEMMCISFKIPAEVLYNDCPAMEDSAEEPAPKRLRLDE 488

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. TrEMBL
Match: A0A1A8JMA3 (tRNA (guanine(37)-N1)-methyltransferase OS=Nothobranchius kuhntae OX=321403 GN=TRMT5 PE=3 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 1.815e-74
Identity = 149/417 (35.73%), Postives = 241/417 (57.79%), Query Frame = 1

Query:   40 LSCTMSQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFS---PDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDI--EFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKS-VNLRFVRNVSPSKDMFCA 1269
            L  TM+   +  L+  P        ++K+A  + I  P L+V       V+K  KK +   P + K  + K  +G ++++ ++LDP +++SI N LS+ E+  L+   +  E  D+     ++ + YDN K   + + +LP  Q +T GF+ +GHI H+NL++   P K +IG++I+DK  GVT VV+K N ID+ +R F +E+LAGEE  V  +KENG  ++FDFS+VYWN RLSTEH RV+   ++ D V+D   GVGPFA+PAA+ G  + ANDLN ES KW + N ++N  + +   +   NLD   FI+  +   +  ++     +   H++MNLP++A++FL A     Q+E     ++   V CY F  ++ P   +  + +  L   +++  ++ FVRNV+P+KDM CA
Sbjct:   27 LCATMNTELEPKLYCPPSGVRGMTSLDKEAFTQTITVPALQVPTGVLNKVVKSLKKSTIQRPGVPKIVQDK--EGNADFRLVLLDPHRVSSI-NSLSEAEAEALRAFGVSEELQDY-----EVKLTYDNLKSEEVLEAVLPQGQDVTSGFSRVGHIAHMNLRDHQLPFKNLIGQVIMDKNPGVTCVVNKTNIIDSTYRNFCMEVLAGEENMVAKVKENGVMYEFDFSRVYWNPRLSTEHQRVVQLFKRGDAVFDVFAGVGPFAVPAARLGANIWANDLNPESYKWLQHNCKLNKVERK---VQTFNLDGRAFIQGPLKQELCTLLKGETSV---HVVMNLPALALEFLDAFRGLLQLEPACEQNL-PTVHCYAFSKEDDPAADVVLRASRSLGFPLENQCSVHFVRNVAPNKDMMCA 428

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. TrEMBL
Match: R7TC87 (tRNA (guanine(37)-N1)-methyltransferase OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_218789 PE=3 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 3.085e-74
Identity = 163/444 (36.71%), Postives = 238/444 (53.60%), Query Frame = 1

Query:   55 SQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKF---SPDLIKFDKSKKFDGT-SEYKGIILDPSKLNSI-----HNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDN-QHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRE----RVKSVNLRFVRNVSPSKDMFCAEIIVNLSAISGNFEA--ESKRRKLD 1338
            S S  + L   P+      +++K A   +   P+L +  +     MK+ K+F    P +   D     D    + K II DP K  S+     H +   +E  +L + D EF D+        + YDN+K   +   +LP+  Q + GF+ IGHI+HLNLKE     K++IG+++L+K S + TVV+K+N ID+ +R FA+E+LAGE+  +TT +ENG QF+ DFS+V+WN RL TEH R+ D LR  DVVYD   G+GPFA+PAAKK C V+ANDLN ES KW   NM++N  K         N+D   FI + + PH+++           H++MNLP++AV+FL A       +      +   V CY F   +   E IE+   T              +R VRNV+P K+M C E  ++ S +  N +A  E KR KL+
Sbjct:    6 SSSEGAVLLRPPQTVRGMTQLDKDAFKVQTKLPVLFIPNDKMHKTMKVLKEFLMKRPGVSAIDDVALNDPRYKKVKRIIFDPEKCRSVDALPDHVKERFQELKMLPETDFEFIDY-------EITYDNWKYEEILQAVLPEEEQKVAGFSRIGHILHLNLKEYHEDYKWLIGQVLLEKFSTIRTVVNKVNTIDSTYRNFAMEVLAGEDDMLTTCRENGLQFRMDFSRVFWNPRLGTEHQRITDQLRSGDVVYDVMAGIGPFAVPAAKKQCCVLANDLNPESYKWLLHNMKLN--KVNGANEQCFNMDGRQFIREQLKPHLMQQQSDEASQCSAHILMNLPALAVEFLDAFQGLCSEQEAASLRLLPTVHCYSFCSGQWGSEMIEQMRGTAALHLGCPLPADAVIRQVRNVAPGKEMLCVEFKLSRSILCSNADAAPEKKRAKLE 440

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Cavefish
Match: trmt5 (tRNA methyltransferase 5 [Source:NCBI gene;Acc:103022759])

HSP 1 Score: 234.187 bits (596), Expect = 3.390e-71
Identity = 146/457 (31.95%), Postives = 256/457 (56.02%), Query Frame = 1

Query:   19 FNFNYLMLSCTMSQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFS---PDLIKF--DKSKKFDGTS-EYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAI--VANDQIEFITRSDVFL-HVKCYCFXXXXXXXXXXXXXXNTQLRERVKS-VNLRFVRNVSPSKDMFCAEIIVNLSAI-SGN----FEAESKRRKLDE 1341
            F+ +  +++   + +   +L+  P       +++++A  + I+   +++  +    V+K  KK +   P L +   D   + D    E++ ++LDP  ++S  +  S  E+  LK   ++     ++  ++ + YDN K   +   +LP+ Q +T GF+ +GHI HLNL++   P + +IG++I+DK  G+T VV+K N ID+ +R F +E+LAGE   V  ++ENG  ++FDF++VYWN RLSTEH RV+  LR+ D V+D   GVGPF++PAA++GC V ANDLN ES +W + N ++N  +++   +   NLD  +FI   +   + E++  S  +   H++MNLP++A++FL A   +   + E     +V L  V CY F  ++ P++ + E+    L   ++    +  VRNV+P+K+M C    +    + S N     E   KR+K +E
Sbjct:   40 FHAHKSVMADPQAPAAHPSLYTAPPAVRGMTELDREAFTQTINVLAIRIPTSVIDKVVKSLKKVALQRPGLKRVVDDPQNQTDSDGKEHRLLLLDPQSVSSPES-FSQDEANALKAFSVKPE---LQRYELKLTYDNLKSDEVLRAVLPEGQDVTSGFSRVGHIAHLNLRDHQLPFRNLIGQVIIDKNPGITCVVNKTNTIDSTYRNFQMEVLAGENNMVAKVRENGVLYEFDFARVYWNPRLSTEHERVVSLLRRGDTVFDIFAGVGPFSVPAARRGCTVFANDLNPESYRWLQHNTKLNKVQAK---VTTFNLDGREFIRGPVRERLPELMKGSAGI---HVVMNLPALALEFLDAFRGLLGPEPEREPSGEVNLPQVHCYGFSKEDDPQKDVVERAEAALGTSLQGRCTVHMVRNVAPNKEMMCVSFTMPTEVLYSSNNKDFSEPSPKRQKCEE 486

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BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Yeast
Match: TRM5 (tRNA(m(1)G37)methyltransferase; methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; localized to both cytoplasm and mitochondria, and modifies both cytoplasmic and mitochondrial tRNAs; mutations in human ortholog TRMT5 are associated with skeletal muscle respiratory chain deficiencies, and trm5 mutations analogous to disease mutations decrease respiration [Source:SGD;Acc:S000001112])

HSP 1 Score: 176.407 bits (446), Expect = 3.846e-50
Identity = 154/509 (30.26%), Postives = 230/509 (45.19%), Query Frame = 1

Query:   10 PFLFNFNYLMLSCTMSQSNQSALWECPKNFYIDGKVNKQAACREID-------FPILKVKINNFKDVMKIFKKFS---------PDLIKFDKSKKFD----------------GTSEYKGIILDPS--KLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDN---QHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGE-EQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDS-LRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKS---------------------EKLPK------------FHLIMNLPSIAVDFL----SAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERV-------------KSVNLRFVRNVSPSKDMFCA 1269
            P    FN L+ SC MS             F  +  VN+Q   RE+D        P+  VK    K++    K F          P ++K + SK  D                 T   KG++L  S   +   + +L +     LK+   E   H  E++   + YD +K   +   +LP+    +  TGFTI GHI HLNL+ EF P   +IG++ILDK + +  VV K++ I T+FRTF ++++AG+ +  V   KE+   FKFDFSKVYWNSRL TEH R++    +   VV D   GVGPFA+PA KK   V+ANDLN ES K+ K N+ +N        +   N+D  DFI +        I D+                      ++ P+             H +MNLP  A+ FL        A+ +    T    ++HV  +CF       +  E++++ ++  R+              +V+L  VR V+P+K M+CA
Sbjct:    6 PVFQKFNRLISSCKMSGV-----------FPYNPPVNRQ--MRELDRSFFITKIPMCAVKFPEPKNISVFSKNFKNCILRVPRIPHVVKLNSSKPKDELTSVQNKKLKTADGNNTPVTKGVLLHESIHSVEDAYGKLPEDALAFLKENSAEIVPH--EYV---LDYDFWKAEEILRAVLPEQFLEEVPTGFTITGHIAHLNLRTEFKPFDSLIGQVILDKNNKIECVVDKVSSIATQFRTFPMKVIAGKSDSLVVEQKESNCTFKFDFSKVYWNSRLHTEHERLVKQYFQPGQVVCDVFAGVGPFAVPAGKKDVIVLANDLNPESYKYLKENIALNKVAKT---VKSFNMDGADFIRQSPQLLQQWIQDEEGGKITIPLPLKKRHRSQQHNDQQPPQPRTKELIIPSHISHYVMNLPDSAISFLGNFRGIFAAHTKGATDTIQMPWVHV--HCFEKYPPGDQVTEDELHARVHARIIAALKVTADDLPLNAVSLHLVRKVAPTKPMYCA 491

[Close]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Nematostella
Match: EDO36767 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHN7])

HSP 1 Score: 180.644 bits (457), Expect = 3.440e-52
Identity = 114/278 (41.01%), Postives = 159/278 (57.19%), Query Frame = 1

Query:  463 TGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVS-GVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFL---SAIVANDQIEFITR-SDVFL----HVKCYCFXXXXXXXXXXXXXXNTQLRERVKSV-NLRFVRNVSPSKDMFC 1266
            +GF  IGHI H+NL++     K IIG++I+DK S  + TVV+K N ID  FR F +E++AGE    T+I +NG  ++FDFSKVYWNSRL  EH R++DS    DV+ D   GVGPFAIPAAKKGC V ANDLN  S K  + N + N    R   I   NLD  +F    ++      + ++ K+   H++MNLP+ A+ FL     + +  + +FI+  S   L     V CYCF   E P    +++    L   ++ +  +  VRNV+P K M C
Sbjct:  118 SGFETIGHIAHVNLRDNQLKFKNIIGQVIMDKNSPQIKTVVNKTNTIDDTFRFFKMEVIAGENNLQTSIIQNGITYEFDFSKVYWNSRLQAEHDRLVDSFSSSDVICDMFAGVGPFAIPAAKKGCFVYANDLNPSSFKALEHNAKTNQVADR---IKAFNLDGREF----VMQVTENTLKENNKMFN-HVVMNLPATALQFLDVFKGLFSGYEDKFISSLSGSTLINLPSVHCYCFSKDENPMMDAQKQAEKVLGASLEGICKVYHVRNVAPKKQMMC 387

[Close]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Medaka
Match: trmt5 (tRNA methyltransferase 5 [Source:NCBI gene;Acc:101175279])

HSP 1 Score: 239.965 bits (611), Expect = 1.689e-73
Identity = 144/405 (35.56%), Postives = 232/405 (57.28%), Query Frame = 1

Query:   76 LWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFS---PDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLT-GFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSA---IVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKS-VNLRFVRNVSPSKDMFC 1266
            L+  P        ++K A  + I  P L+V       VMK  KK +   P + +  + K  +G S+++ I+LDP+++ S  +  SD E+  L+   +    H  E   + + YDN K   + + +LP+ Q +T GF+ +GHI H+NL++     K +IG++I+DK  G+T VV+K N ID+ +R F +E+LAGEE  V  +KENG  ++FDFS+VYWN RLSTEH RV+  +++ D ++D   GVGPF IPAA++G  + ANDLN ES +W + N  +N  +SR   +   NLD  +FI   +   +  +V     +   H++MNLP++A++FL A   ++ N+    +    V+    CY F   E P   + ++ ++ L   +++   + FVRNV+P+KDM C
Sbjct:   45 LYRPPPEVRGMTSLDKDAFTQTITVPALRVPTVVINKVMKSLKKATIQRPGVSRVVQDK--EGNSDFRLILLDPNRMAS-SSCFSDAEAEALRSFSVSDELHNFE---LKLTYDNLKTEEVLEAVLPEGQDVTSGFSRVGHIAHMNLRDHQLLYKNLIGQVIMDKTPGITCVVNKTNTIDSTYRNFKMEVLAGEENMVAKVKENGVTYEFDFSRVYWNPRLSTEHERVVQLVKRGDTMFDVFAGVGPFTIPAARRGANIFANDLNPESYRWLQHNSRLNKVESR---VRTFNLDGREFIRGPLKQELPALVKGGAGV---HVVMNLPALALEFLDAFRGLLKNEPPCDLNLPTVY----CYGFSKDEDPGADMVKRASSSLGFPLENKSTVHFVRNVAPNKDMMC 433

[Close]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Planmine SMEST
Match: SMESG000019159.1 (SMESG000019159.1)

HSP 1 Score: 823.928 bits (2127), Expect = 0.000e+0
Identity = 432/435 (99.31%), Postives = 433/435 (99.54%), Query Frame = 1

Query:   37 MLSCTMSQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKSVNLRFVRNVSPSKDMFCAEIIVNLSAISGNFEAESKRRKLDE 1341
            MLSCTMSQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDAC GVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFIEKIMIPHIIEIVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIE+ITRSDVFLHV CYCFIGKEIPKETIEEKINTQLRERVKSVNLRFVRNVSPSKDMFCAEIIVNLSAISGNFEAESKRRKLDE
Sbjct:    1 MLSCTMSQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACAGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFIEKIMIPHIIEIVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEYITRSDVFLHVNCYCFIGKEIPKETIEEKINTQLRERVKSVNLRFVRNVSPSKDMFCAEIIVNLSAISGNFEAESKRRKLDE 435

[Close]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Planmine SMEST
Match: SMESG000019159.1 (SMESG000019159.1)

HSP 1 Score: 811.601 bits (2095), Expect = 0.000e+0
Identity = 427/430 (99.30%), Postives = 428/430 (99.53%), Query Frame = 1

Query:   52 MSQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKSVNLRFVRNVSPSKDMFCAEIIVNLSAISGNFEAESKRRKLDE 1341
            MSQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDAC GVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFIEKIMIPHIIEIVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIE+ITRSDVFLHV CYCFIGKEIPKETIEEKINTQLRERVKSVNLRFVRNVSPSKDMFCAEIIVNLSAISGNFEAESKRRKLDE
Sbjct:    1 MSQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACAGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFIEKIMIPHIIEIVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEYITRSDVFLHVNCYCFIGKEIPKETIEEKINTQLRERVKSVNLRFVRNVSPSKDMFCAEIIVNLSAISGNFEAESKRRKLDE 430

[Close]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Planmine SMEST
Match: SMESG000019155.1 (SMESG000019155.1)

HSP 1 Score: 416.001 bits (1068), Expect = 4.279e-142
Identity = 220/427 (51.52%), Postives = 300/427 (70.26%), Query Frame = 1

Query:   37 MLSCTMS-----QSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKV-SGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKSVNLRFVRNVSPSKDMFCAEIIVNLSAIS 1299
            +LSC+       + N   L++ P NF  +GK+NK    R+I+FP+  V    FK V  IF +FSP+L KF+           KGIILDP+    I+NEL+D+E   LK+++ EFND  I F +  V Y+NY L TL +W    N  L   + +GHI+H+NL+E+  P + +IGE+ILD     + TVV KLN +D+EFR   L+LLAG+  YVTT+KEN  QFK D+SKVYWNSRLSTEH RV+  L+  D+VYDAC GVGPFAIPAAKKGC+V+ANDLN E C+WF+TN+E+NS K   + ID+HN+DA DFIEK++IP IIE  +K++ L  F+LIMN PS AV+FLS+IV N++++ IT S+V L+V+CYCFIG+E+ ++ + ++I +    R+K++N RFVRNVSPSKD +C+EI VNLS I+
Sbjct:   47 LLSCSYKVLKSFRRNYILLYQAPHNFLQNGKINKNVVSRDIEFPVFNVPSLYFKKVQTIFSRFSPNLRKFENVVINPNDKNQKGIILDPNNFEKINNELTDQEIQFLKEINCEFNDSKINFSRFTVSYNNYFLETLLEWAFDKNTELNSRSFVGHIIHINLQEKHLPYQNLIGELILDYYHPRIKTVVGKLNGVDSEFRNAELKLLAGKPDYVTTVKENASQFKLDYSKVYWNSRLSTEHLRVVSMLKSGDIVYDACAGVGPFAIPAAKKGCRVIANDLNSECCQWFRTNVELNSTKKYQISIDMHNMDAKDFIEKVVIPDIIETANKNKDLTSFYLIMNHPSKAVEFLSSIVTNNKLDKITHSNVILNVQCYCFIGREVTQQVVRDQIISHFGNRMKNLNFRFVRNVSPSKDFYCSEISVNLSPIN 473

[Close]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Planmine SMEST
Match: SMESG000019155.1 (SMESG000019155.1)

HSP 1 Score: 416.387 bits (1069), Expect = 4.724e-142
Identity = 220/427 (51.52%), Postives = 300/427 (70.26%), Query Frame = 1

Query:   37 MLSCTMS-----QSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKDVMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESVILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLTGFTIIGHIVHLNLKEEFYPLKYIIGEMILDKV-SGVTTVVSKLNKIDTEFRTFALELLAGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDACTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLHNLDAGDFXXXXXXXXXXXXVDKSEKLPKFHLIMNLPSIAVDFLSAIVANDQIEFITRSDVFLHVKCYCFXXXXXXXXXXXXXXNTQLRERVKSVNLRFVRNVSPSKDMFCAEIIVNLSAIS 1299
            +LSC+       + N   L++ P NF  +GK+NK    R+I+FP+  V    FK V  IF +FSP+L KF+           KGIILDP+    I+NEL+D+E   LK+++ EFND  I F +  V Y+NY L TL +W    N  L   + +GHI+H+NL+E+  P + +IGE+ILD     + TVV KLN +D+EFR   L+LLAG+  YVTT+KEN  QFK D+SKVYWNSRLSTEH RV+  L+  D+VYDAC GVGPFAIPAAKKGC+V+ANDLN E C+WF+TN+E+NS K   + ID+HN+DA DFIEK++IP IIE  +K++ L  F+LIMN PS AV+FLS+IV N++++ IT S+V L+V+CYCFIG+E+ ++ + ++I +    R+K++N RFVRNVSPSKD +C+EI VNLS I+
Sbjct:   55 LLSCSYKVLKSFRRNYILLYQAPHNFLQNGKINKNVVSRDIEFPVFNVPSLYFKKVQTIFSRFSPNLRKFENVVINPNDKNQKGIILDPNNFEKINNELTDQEIQFLKEINCEFNDSKINFSRFTVSYNNYFLETLLEWAFDKNTELNSRSFVGHIIHINLQEKHLPYQNLIGELILDYYHPRIKTVVGKLNGVDSEFRNAELKLLAGKPDYVTTVKENASQFKLDYSKVYWNSRLSTEHLRVVSMLKSGDIVYDACAGVGPFAIPAAKKGCRVIANDLNSECCQWFRTNVELNSTKKYQISIDMHNMDAKDFIEKVVIPDIIETANKNKDLTSFYLIMNHPSKAVEFLSSIVTNNKLDKITHSNVILNVQCYCFIGREVTQQVVRDQIISHFGNRMKNLNFRFVRNVSPSKDFYCSEISVNLSPIN 481

The following BLAST results are available for this feature:

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Human
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Celegans
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Fly
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Zebrafish
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Xenopus
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Mouse
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. UniProt/SwissProt
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. TrEMBL
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Cavefish
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Sea Lamprey
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Yeast
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Nematostella
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Medaka
BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Planmine SMEST

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1


Match Name	E-value	Identity	Description
TRMT5	9.472e-73	34.77	tRNA methyltransferase 5 [Source:HGNC Symbol;Acc:H...	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
C53A5.17	8.936e-60	33.56	tRNA (guanine(37)-N1)-methyltransferase [Source:U...	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
CG32281	3.414e-67	33.98	gene:FBgn0052281 transcript:FBtr0073034	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
trmt5	1.696e-75	34.11	tRNA methyltransferase 5 [Source:NCBI gene;Acc:564...	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Trmt5	2.663e-70	32.84	TRM5 tRNA methyltransferase 5 [Source:MGI Symbol;A...	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|F6VSS6\|TRM5_XENTR	9.018e-77	36.99	tRNA (guanine(37)-N1)-methyltransferase OS=Xenopus...	[more]
sp\|B8A5G9\|TRM5_DANRE	1.280e-74	34.11	tRNA (guanine(37)-N1)-methyltransferase OS=Danio r...	[more]
sp\|Q3MHN8\|TRM5_BOVIN	1.126e-73	35.80	tRNA (guanine(37)-N1)-methyltransferase OS=Bos tau...	[more]
sp\|F7GSQ4\|TRM5_MACMU	8.494e-72	34.65	tRNA (guanine(37)-N1)-methyltransferase OS=Macaca ...	[more]
sp\|Q95KJ2\|TRM5_MACFA	7.589e-71	34.42	tRNA (guanine(37)-N1)-methyltransferase OS=Macaca ...	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4W3GUF6	4.545e-75	37.07	tRNA (guanine(37)-N1)-methyltransferase OS=Callorh...	[more]
V9KPY0	8.866e-75	37.07	tRNA (guanine(37)-N1)-methyltransferase OS=Callorh...	[more]
A0A4W3GR83	1.035e-74	37.07	tRNA (guanine(37)-N1)-methyltransferase OS=Callorh...	[more]
A0A1A8JMA3	1.815e-74	35.73	tRNA (guanine(37)-N1)-methyltransferase OS=Nothobr...	[more]
R7TC87	3.085e-74	36.71	tRNA (guanine(37)-N1)-methyltransferase OS=Capitel...	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
trmt5	3.390e-71	31.95	tRNA methyltransferase 5 [Source:NCBI gene;Acc:103...	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
TRM5	3.846e-50	30.26	tRNA(m(1)G37)methyltransferase; methylates a tRNA ...	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO36767	3.440e-52	41.01	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
trmt5	1.689e-73	35.56	tRNA methyltransferase 5 [Source:NCBI gene;Acc:101...	[more]

BLAST of tRNA (guanine(37)-N1)-methyltransferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4


Match Name	E-value	Identity	Description
SMESG000019159.1	0.000e+0	99.31	SMESG000019159.1	[more]
SMESG000019159.1	0.000e+0	99.30	SMESG000019159.1	[more]
SMESG000019155.1	4.279e-142	51.52	SMESG000019155.1	[more]
SMESG000019155.1	4.724e-142	51.52	SMESG000019155.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30035364 ID=SMED30035364|Name=tRNA (guanine(37)-N1)-methyltransferase|organism=Schmidtea mediterranea sexual|type=transcript|length=1401bp
TAATTAAGTCCTTTCCTTTTTAATTTTAATTATCTTATGTTATCTTGTAC
AATGTCACAATCAAATCAATCGGCATTATGGGAATGTCCTAAAAATTTTT
ATATTGATGGAAAAGTCAATAAACAAGCAGCATGTCGTGAAATCGATTTC
CCTATTTTAAAAGTTAAAATAAATAACTTTAAGGATGTCATGAAAATATT
TAAAAAATTCTCTCCTGACCTTATTAAATTTGATAAATCGAAAAAGTTTG
ATGGTACTTCTGAGTATAAAGGTATTATTTTAGATCCTTCTAAACTCAAC
AGTATCCACAATGAACTCAGTGATAAAGAATCTGTAATTCTTAAACAGTT
GGACATTGAATTTAATGATCATATCATTGAATTTATGAAGATTAATGTTG
TATATGACAATTATAAGTTATCAACTCTATTCGATTGGATTTTACCCGAT
AACCAACATTTAACTGGATTTACTATTATCGGTCATATAGTACATTTAAA
TTTAAAAGAAGAGTTTTATCCTTTGAAATACATTATCGGAGAAATGATAT
TAGATAAAGTATCCGGAGTTACTACAGTTGTTTCCAAACTAAATAAAATA
GACACTGAATTTCGAACATTTGCATTGGAACTGTTAGCCGGGGAAGAGCA
ATATGTTACAACTATTAAAGAAAACGGTTTTCAATTTAAATTTGACTTTT
CAAAGGTCTATTGGAACTCAAGATTGAGCACAGAACATTGTCGAGTTATT
GACTCACTACGAAAAGATGATGTAGTGTATGATGCATGTACCGGTGTTGG
ACCATTTGCTATTCCTGCTGCCAAGAAAGGCTGTAAGGTGGTTGCAAATG
ATCTCAACGATGAGAGCTGTAAATGGTTCAAAACGAATATGGAAATAAAT
TCCAAGAAATCCCGTCATTTACCGATTGATTTGCACAATCTCGATGCTGG
AGATTTCATTGAGAAAATAATGATTCCTCACATAATTGAAATTGTTGATA
AAAGCGAAAAATTGCCGAAATTTCATCTAATCATGAATTTGCCGTCGATT
GCTGTTGATTTTCTTTCCGCAATTGTTGCTAATGATCAAATCGAGTTTAT
AACTCGGTCTGATGTCTTTCTCCATGTCAAATGTTACTGTTTTATTGGTA
AAGAAATTCCAAAAGAAACGATTGAAGAGAAAATCAATACTCAGCTCAGG
GAAAGAGTGAAATCTGTTAATCTTCGATTTGTGCGAAACGTTTCTCCATC
AAAAGATATGTTTTGCGCCGAAATAATCGTCAATTTGTCTGCGATATCTG
GGAACTTTGAAGCGGAATCAAAACGAAGGAAATTGGACGAATAAATTCTT
TAGAATCCGTCTTTTCATTTTTTCAATATTTCTTCAGATTCTGAATTTCT
C

protein sequence of SMED30035364-orf-1

>SMED30035364-orf-1 ID=SMED30035364-orf-1|Name=SMED30035364-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=436bp
MLSCTMSQSNQSALWECPKNFYIDGKVNKQAACREIDFPILKVKINNFKD
VMKIFKKFSPDLIKFDKSKKFDGTSEYKGIILDPSKLNSIHNELSDKESV
ILKQLDIEFNDHIIEFMKINVVYDNYKLSTLFDWILPDNQHLTGFTIIGH
IVHLNLKEEFYPLKYIIGEMILDKVSGVTTVVSKLNKIDTEFRTFALELL
AGEEQYVTTIKENGFQFKFDFSKVYWNSRLSTEHCRVIDSLRKDDVVYDA
CTGVGPFAIPAAKKGCKVVANDLNDESCKWFKTNMEINSKKSRHLPIDLH
NLDAGDFIEKIMIPHIIEIVDKSEKLPKFHLIMNLPSIAVDFLSAIVAND
QIEFITRSDVFLHVKCYCFIGKEIPKETIEEKINTQLRERVKSVNLRFVR
NVSPSKDMFCAEIIVNLSAISGNFEAESKRRKLDE*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **INTERPRO**
Term	Definition
IPR030382	MeTrfase_TRM5/TYW2
IPR029063	SAM-dependent_MTases

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005759	mitochondrial matrix

Vocabulary: **biological process**
Term	Definition
GO:0008033	tRNA processing
GO:0030488	tRNA methylation
GO:0032259	methylation

Vocabulary: **molecular function**
Term	Definition
GO:0008168	methyltransferase activity
GO:0009019	tRNA (guanine-N1-)-methyltransferase activity
GO:0016740	transferase activity
GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.30.300.110		coord: 110..202 e-value: 1.2E-18 score: 69.1
None	No IPR available	GENE3D	G3DSA:3.40.50.150		coord: 206..416 e-value: 4.5E-44 score: 152.4
None	No IPR available	PANTHER	PTHR23245	UNCHARACTERIZED	coord: 13..416
None	No IPR available	PANTHER	PTHR23245:SF36	TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE	coord: 13..416
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PFAM	PF02475	Met_10	coord: 144..367 e-value: 6.4E-54 score: 182.7
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PROSITE	PS51684	SAM_MT_TRM5_TYW2	coord: 145..417 score: 89.308
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	SSF53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 143..415

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tRNA (guanine(37)-N1)-methyltransferase

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Owlery

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