Surfeit locus protein 2

Overview
NameSurfeit locus protein 2
Smed IDSMED30035347
Length (bp)642
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Surfeit locus protein 2 (SMED30035347) t-SNE clustered cells

Violin plots show distribution of expression levels for Surfeit locus protein 2 (SMED30035347) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Surfeit locus protein 2 (SMED30035347) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Surfeit locus protein 2 (SMED30035347) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035347

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30035347SMESG000073153.1 SmedASXL_008730SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30035347SMESG000073153.1 dd_Smed_v4_4458_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Surfeit locus protein 2 vs. Ensembl Human
Match: SURF2 (surfeit 2 [Source:HGNC Symbol;Acc:HGNC:11475])

HSP 1 Score: 57.3806 bits (137), Expect = 1.965e-9
Identity = 35/130 (26.92%), Postives = 63/130 (48.46%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFN-CARTEHIFKQYDKF----LRKTKD-NEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKK 401
            +  ++R  +  +  L    D  KV+C LTGHE+      +  Y +GKK+    R    F  Y +F    +  TK+ ++++C+LT + I    + +  H  G  + RA  K+ +C++   EY P   V ++
Sbjct:    4 LPGDVRAFLREHPSLRLQTDARKVRCILTGHELPCRLPELQVYTRGKKYQRLVRASPAF-DYAEFEPHIVPSTKNPHQLFCKLTLRHINKCPEHVLRHTQGRRYQRALCKYEECQKQGVEYVPACLVHRR 132
[Close]
BLAST of Surfeit locus protein 2 vs. Ensembl Human
Match: SURF2 (surfeit 2 [Source:HGNC Symbol;Acc:HGNC:11475])

HSP 1 Score: 57.3806 bits (137), Expect = 1.965e-9
Identity = 35/130 (26.92%), Postives = 63/130 (48.46%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFN-CARTEHIFKQYDKF----LRKTKD-NEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKK 401
            +  ++R  +  +  L    D  KV+C LTGHE+      +  Y +GKK+    R    F  Y +F    +  TK+ ++++C+LT + I    + +  H  G  + RA  K+ +C++   EY P   V ++
Sbjct:    4 LPGDVRAFLREHPSLRLQTDARKVRCILTGHELPCRLPELQVYTRGKKYQRLVRASPAF-DYAEFEPHIVPSTKNPHQLFCKLTLRHINKCPEHVLRHTQGRRYQRALCKYEECQKQGVEYVPACLVHRR 132
[Close]
BLAST of Surfeit locus protein 2 vs. Ensembl Zebrafish
Match: surf2 (surfeit 2 [Source:NCBI gene;Acc:445173])

HSP 1 Score: 53.1434 bits (126), Expect = 3.507e-8
Identity = 31/128 (24.22%), Postives = 65/128 (50.78%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFN--CARTEHIFKQYDKFLRKT--KDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKK 401
            + E++R  ++     E  E   ++KC L GHE   +   +  +  GKK+    A+ +  + QY+  +  +  + N ++C+LT + I    K++  H++G  + +A  ++ +C     ++ PV  +RKK
Sbjct:    4 LPEDLRSFLEKQPCFELTES-KRIKCVLNGHEFPCSLSELQHFTSGKKYKKLSAQEQFNYSQYEPHITSSSKQPNHLFCKLTLRHINREPKEVLRHVSGKRYQKALAQYEECVRQGVKFVPV-QLRKK 129
[Close]
BLAST of Surfeit locus protein 2 vs. Ensembl Xenopus
Match: gxylt1 (glucoside xylosyltransferase 1 [Source:Xenbase;Acc:XB-GENE-5867993])

HSP 1 Score: 60.4622 bits (145), Expect = 1.278e-10
Identity = 34/123 (27.64%), Postives = 62/123 (50.41%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIFKQY----DKFLRKTKDN-EVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPV 383
            +  ++R  +  +  L+ I   NKV+CTLTGHE+I     +  + +GKK+    T      +       +  TK+  +++C+LT + I    + I  H+ G  + RA +K+ +CK+   E+ P 
Sbjct:    4 LPGDVRLFLQQHPSLQLIA-ANKVRCTLTGHELICRLPELQSFTRGKKYQRLTTASSSFDFSSFEPHIVPSTKNPKQLFCKLTLRHINRIPEHIQRHVQGKRYTRALKKYEECKQQGVEFVPA 125
[Close]
BLAST of Surfeit locus protein 2 vs. Ensembl Xenopus
Match: gxylt1 (glucoside xylosyltransferase 1 [Source:Xenbase;Acc:XB-GENE-5867993])

HSP 1 Score: 53.5286 bits (127), Expect = 3.311e-8
Identity = 29/102 (28.43%), Postives = 55/102 (53.92%), Query Frame = 3 
Query:   93 NKVKCTLTGHEMIMNKDIIDKYLKGKKFN---CARTEHIFKQYDKFLRKTKDN--EVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPV 383
            ++V+CTLTGHE+I     +  + +GKK+     A +   F  ++  +  +  N  +++C+LT + I    + I  H+ G  + RA +K+ +CK+   E+ P 
Sbjct:   10 SQVRCTLTGHELICRLPELQSFTRGKKYQRLTTASSSFDFSSFEPHIVPSTKNPKQLFCKLTLRHINRIPEHIQRHVQGKRYTRALKKYEECKQQGVEFVPA 111
[Close]
BLAST of Surfeit locus protein 2 vs. Ensembl Mouse
Match: Surf2 (surfeit gene 2 [Source:MGI Symbol;Acc:MGI:98444])

HSP 1 Score: 58.9214 bits (141), Expect = 3.912e-10
Identity = 34/143 (23.78%), Postives = 70/143 (48.95%), Query Frame = 3 
Query:   39 EIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIFKQYD------KFLRKTKD-NEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKKDLGKYMSMMKELNGE 446
            ++   +  +  L  + +  KV+C+LTGHE+      + +Y +GKK+   R    F  +D        +  TK+ ++++C+LT + I  + + +  H  G  + RA  ++ +C++   EY P   + K+   +  +   EL G+
Sbjct:    7 DVLAFLRQHPSLRLLPNTRKVRCSLTGHELPCRLPELQEYTRGKKYQ--RLSSSFSNFDYAAFEPHIVPSTKNRHQLFCKLTLRHINKSPEHVLRHTQGRRYQRALHQYEECQKQGVEYVPACLLHKRKKREDQTNSDELPGQ 147
[Close]
BLAST of Surfeit locus protein 2 vs. UniProt/SwissProt
Match: sp|O57589|SURF2_TAKRU (Surfeit locus protein 2 OS=Takifugu rubripes OX=31033 GN=surf2 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.673e-10
Identity = 41/144 (28.47%), Postives = 73/144 (50.69%), Query Frame = 3 
Query:   81 IEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIF--KQYDKFLRKT--KDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTV--RKKDLGKYMS---MMKELNGENGEESEKDNGEIE 485
            + D  K+KCTL GHE   N   ++K+ +GKK+   R    F  +QY+  +  +  + N+++C+LT + +      +  HI+G  F +A  K+  C +   E+ P   +  R KD  + +S     K+ NG     S +++G+ E
Sbjct:   20 LTDGKKIKCTLNGHEFPCNLQELEKFTQGKKYEKLRPAADFNYRQYEPHIVASTKQPNQLFCKLTLRHLNRQPHHVLRHIHGKRFKKALSKYEDCVQKGIEFIPARLMQKRPKDAREEVSRGRTSKQGNGTWAPSSSEEDGDSE 163
[Close]
BLAST of Surfeit locus protein 2 vs. UniProt/SwissProt
Match: sp|P09926|SURF2_MOUSE (Surfeit locus protein 2 OS=Mus musculus OX=10090 GN=Surf2 PE=1 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.736e-9
Identity = 34/143 (23.78%), Postives = 70/143 (48.95%), Query Frame = 3 
Query:   39 EIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIFKQYD------KFLRKTKD-NEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKKDLGKYMSMMKELNGE 446
            ++   +  +  L  + +  KV+C+LTGHE+      + +Y +GKK+   R    F  +D        +  TK+ ++++C+LT + I  + + +  H  G  + RA  ++ +C++   EY P   + K+   +  +   EL G+
Sbjct:    7 DVLAFLRQHPSLRLLPNTRKVRCSLTGHELPCRLPELQEYTRGKKYQ--RLSSSFSNFDYAAFEPHIVPSTKNRHQLFCKLTLRHINKSPEHVLRHTQGRRYQRALHQYEECQKQGVEYVPACLLHKRKKREDQTNSDELPGQ 147
[Close]
BLAST of Surfeit locus protein 2 vs. UniProt/SwissProt
Match: sp|Q15527|SURF2_HUMAN (Surfeit locus protein 2 OS=Homo sapiens OX=9606 GN=SURF2 PE=1 SV=3)

HSP 1 Score: 57.3806 bits (137), Expect = 9.347e-9
Identity = 35/130 (26.92%), Postives = 63/130 (48.46%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFN-CARTEHIFKQYDKF----LRKTKD-NEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKK 401
            +  ++R  +  +  L    D  KV+C LTGHE+      +  Y +GKK+    R    F  Y +F    +  TK+ ++++C+LT + I    + +  H  G  + RA  K+ +C++   EY P   V ++
Sbjct:    4 LPGDVRAFLREHPSLRLQTDARKVRCILTGHELPCRLPELQVYTRGKKYQRLVRASPAF-DYAEFEPHIVPSTKNPHQLFCKLTLRHINKCPEHVLRHTQGRRYQRALCKYEECQKQGVEYVPACLVHRR 132
[Close]
BLAST of Surfeit locus protein 2 vs. TrEMBL
Match: A0A4S2KES2 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_011113 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 2.567e-16
Identity = 50/161 (31.06%), Postives = 80/161 (49.69%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIFKQYDKFL-----RKTKDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKKDLGKY--MSMMKELNGENGEESEKDNGEIETP 491
            M+  + +L+ S+E L  + D N++KC  TGHEM  + D+I +YL G KF  A    + + +  ++      K   N+++C+LT ++I      +  H  G  F +A  K+ +CKES +E+ P +       GKY   S  KE N     E E   G  + P
Sbjct:    1 MDSRLEELLQSSESLVILPDRNRIKCLYTGHEMPFDYDVISRYLSGVKFRRACANKLLENHKDYIVDVSSSKAHLNQLFCKLTWRYINKDVDHLIRHFRGRKFQQALGKYLQCKESGNEFAPTS-------GKYKNASYFKESNRIGTAELESVGGSPKQP 154
[Close]
BLAST of Surfeit locus protein 2 vs. TrEMBL
Match: A0A4S2KFB6 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_011113 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 7.456e-16
Identity = 53/174 (30.46%), Postives = 88/174 (50.57%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIFKQYDKFL-----RKTKDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKKDLGKY--MSMMKELNGENGEESEKD--NGEIETPKEVLSESESVE 524
            M+  + +L+ S+E L  + D N++KC  TGHEM  + D+I +YL G KF  A    + + +  ++      K   N+++C+LT ++I      +  H  G  F +A  K+ +CKES +E+ P +       GKY   S  KE N      S++   NG + T +   S S S++
Sbjct:    1 MDSRLEELLQSSESLVILPDRNRIKCLYTGHEMPFDYDVISRYLSGVKFRRACANKLLENHKDYIVDVSSSKAHLNQLFCKLTWRYINKDVDHLIRHFRGRKFQQALGKYLQCKESGNEFAPTS-------GKYKNASYFKESNRILNSSSQRSTINGSL-TNRRSCSSSPSMK 166
[Close]
BLAST of Surfeit locus protein 2 vs. TrEMBL
Match: A0A4S2KER1 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_011113 PE=4 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 5.150e-15
Identity = 46/144 (31.94%), Postives = 75/144 (52.08%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIFKQYDKFL-----RKTKDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKKDLGKY--MSMMKELN 440
            M+  + +L+ S+E L  + D N++KC  TGHEM  + D+I +YL G KF  A    + + +  ++      K   N+++C+LT ++I      +  H  G  F +A  K+ +CKES +E+ P +       GKY   S  KE N
Sbjct:    1 MDSRLEELLQSSESLVILPDRNRIKCLYTGHEMPFDYDVISRYLSGVKFRRACANKLLENHKDYIVDVSSSKAHLNQLFCKLTWRYINKDVDHLIRHFRGRKFQQALGKYLQCKESGNEFAPTS-------GKYKNASYFKESN 137
[Close]
BLAST of Surfeit locus protein 2 vs. TrEMBL
Match: V4ALB3 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_230039 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 5.639e-13
Identity = 45/135 (33.33%), Postives = 73/135 (54.07%), Query Frame = 3 
Query:   18 KHVK-MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIF---KQYDKFLRKTK---DNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKK 401
            KHV  ++ E+ +L+     LE  ++ NK+KC L+GHEM   +  I  Y+ GKKF   + +  +   +  D  +  TK   +  +YC LT   +  T KDI  HING  F RA  +W +C+E+  ++ P   ++K+
Sbjct:    6 KHVGTLDTEVEELLKKYRELELDKETNKIKCCLSGHEMPSTELAITTYISGKKFLKLKAQSDYNYDEHADHLVPSTKKFHEKHLYCRLTQIHVNHTPKDIDRHINGRKFKRALREWKRCQETGEKFKPFGNLQKQ 140
[Close]
BLAST of Surfeit locus protein 2 vs. TrEMBL
Match: A0A0L8H114 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22024602mg PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.738e-12
Identity = 41/122 (33.61%), Postives = 69/122 (56.56%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIFKQYDKFLRKTKD-------NEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEY 374
            ++ EI+K++  N HL+++ D  K+KC+L+GHEM  +  +++ YL GKK    +  + +  YDK+ +   D       N++ C LT K+I      I  HING  F +A +KW + ++   EY
Sbjct:   11 LSAEIQKILSENSHLKYLSDTGKIKCSLSGHEMSCDAKVVNSYLHGKKHKRLKALNDY-DYDKYRQHLVDSTKRFPKNQLLCLLTYKYINNLQSSIQKHINGRKFQKAFKKWEELQKEGKEY 131
[Close]
BLAST of Surfeit locus protein 2 vs. Ensembl Cavefish
Match: surf2 (surfeit 2 [Source:NCBI gene;Acc:103028662])

HSP 1 Score: 60.4622 bits (145), Expect = 8.837e-11
Identity = 36/128 (28.12%), Postives = 65/128 (50.78%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFN--CARTEHIFKQYDKFLRKT--KDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKK 401
            ++ E+RK + +   LE + D  KVKCTL GHE   N   +  ++ G K+    A  E  + QY+  L  +  + N ++C+LT + +    + +  HING  + +A  ++ +C +   ++ P     KK
Sbjct:    4 LSAELRKFLQNQPFLE-LTDGKKVKCTLNGHEFPCNLTELQHFISGNKYKKLSANAEFNYSQYEPHLVPSTKQPNHLFCKLTLRHLNRVPEHVLRHINGKRYKKALGEYEECVKQGVQFVPARLRHKK 130
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BLAST of Surfeit locus protein 2 vs. Ensembl Cavefish
Match: surf2 (surfeit 2 [Source:NCBI gene;Acc:103028662])

HSP 1 Score: 58.9214 bits (141), Expect = 2.935e-10
Identity = 36/129 (27.91%), Postives = 65/129 (50.39%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFN--CARTEHIFKQYDKFLRKT--KDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKKD 404
            ++ E+RK + +   LE + D  KVKCTL GHE   N   +  ++ G K+    A  E  + QY+  L  +  + N ++C+LT + +    + +  HING  + +A  ++ +C +   ++ P     KK 
Sbjct:    4 LSAELRKFLQNQPFLE-LTDGKKVKCTLNGHEFPCNLTELQHFISGNKYKKLSANAEFNYSQYEPHLVPSTKQPNHLFCKLTLRHLNRVPEHVLRHINGKRYKKALGEYEECVKQGVQFVPARLRHKKQ 131
[Close]
BLAST of Surfeit locus protein 2 vs. Ensembl Nematostella
Match: EDO43393 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYV9])

HSP 1 Score: 56.6102 bits (135), Expect = 6.270e-10
Identity = 35/111 (31.53%), Postives = 59/111 (53.15%), Query Frame = 3 
Query:   42 IRKLIDSNEHLEFIED---LNKVKCTLTGHEMIMNKDIIDKYLKGKKFN--CARTE---HIFKQYDKFLRKT--KDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKW 344
            ++K++  + +LE +E+     KV+C LTGHE+      ID Y++GKK+   C        I  ++ KFL  +  + N+ YC+LT +++      I  H  G  F +A EK+
Sbjct:    8 VKKILAKHPNLEIVEENSERPKVRCKLTGHELPCQPAAIDTYIRGKKYQRLCGSEPKEVAIKDEWRKFLDVSVKRRNQYYCKLTKRYLNNLPHHIERHFAGKRFQKALEKY 118
[Close]
BLAST of Surfeit locus protein 2 vs. Ensembl Medaka
Match: surf2 (surfeit 2 [Source:NCBI gene;Acc:101158543])

HSP 1 Score: 57.3806 bits (137), Expect = 8.628e-10
Identity = 32/121 (26.45%), Postives = 64/121 (52.89%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFN--CARTEHIFKQYDKFLRKT--KDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTP 380
            +  ++R  + +    E + D  K+KCTL GHE+  N   +  + +GKK+    +R E  + QY+  +  +  + N+++C+LT + +    + +  HING  F +A  ++ +C +   E+ P
Sbjct:    4 LPADLRAFLVTQPFFE-LTDNKKIKCTLNGHELPCNLTELQNFTEGKKYKKLSSRAEFNYSQYEPHIIPSTKQPNQLFCKLTLRHLNREPRHVLIHINGKRFKKALAQYEECVKQGIEFVP 123
[Close]
BLAST of Surfeit locus protein 2 vs. Planmine SMEST
Match: SMESG000073153.1 (SMESG000073153.1)

HSP 1 Score: 361.303 bits (926), Expect = 7.394e-129
Identity = 197/198 (99.49%), Postives = 197/198 (99.49%), Query Frame = 3 
Query:   30 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIFKQYDKFLRKTKDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKKDLGKYMSMMKELNGENGEESEKDNGEIETPKEVLSESESVEDNEERMXXXXXXXXXXXXXXXXXVPIKKRKMIN 623
            MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIFKQYDKFLRKTKDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKKDLGKYMSMMKELNGENGEESEKDNGEIE PKEVLSESESVEDNEERMEADDSSEKSNEDESEQEVPIKKRKMIN
Sbjct:    1 MNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIIDKYLKGKKFNCARTEHIFKQYDKFLRKTKDNEVYCELTCKFIKFTYKDIYNHINGYNFLRACEKWFKCKESKSEYTPVTTVRKKDLGKYMSMMKELNGENGEESEKDNGEIEIPKEVLSESESVEDNEERMEADDSSEKSNEDESEQEVPIKKRKMIN 198
The following BLAST results are available for this feature:
BLAST of Surfeit locus protein 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
SURF21.965e-926.92surfeit 2 [Source:HGNC Symbol;Acc:HGNC:11475][more]
SURF21.965e-926.92surfeit 2 [Source:HGNC Symbol;Acc:HGNC:11475][more]
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BLAST of Surfeit locus protein 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Surfeit locus protein 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Surfeit locus protein 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
surf23.507e-824.22surfeit 2 [Source:NCBI gene;Acc:445173][more]
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BLAST of Surfeit locus protein 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gxylt11.278e-1027.64glucoside xylosyltransferase 1 [Source:Xenbase;Acc... [more]
gxylt13.311e-828.43glucoside xylosyltransferase 1 [Source:Xenbase;Acc... [more]
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BLAST of Surfeit locus protein 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Surf23.912e-1023.78surfeit gene 2 [Source:MGI Symbol;Acc:MGI:98444][more]
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BLAST of Surfeit locus protein 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|O57589|SURF2_TAKRU3.673e-1028.47Surfeit locus protein 2 OS=Takifugu rubripes OX=31... [more]
sp|P09926|SURF2_MOUSE2.736e-923.78Surfeit locus protein 2 OS=Mus musculus OX=10090 G... [more]
sp|Q15527|SURF2_HUMAN9.347e-926.92Surfeit locus protein 2 OS=Homo sapiens OX=9606 GN... [more]
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BLAST of Surfeit locus protein 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2KES22.567e-1631.06Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4S2KFB67.456e-1630.46Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4S2KER15.150e-1531.94Uncharacterized protein OS=Opisthorchis felineus O... [more]
V4ALB35.639e-1333.33Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A0L8H1141.738e-1233.61Uncharacterized protein OS=Octopus bimaculoides OX... [more]
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BLAST of Surfeit locus protein 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
surf28.837e-1128.13surfeit 2 [Source:NCBI gene;Acc:103028662][more]
surf22.935e-1027.91surfeit 2 [Source:NCBI gene;Acc:103028662][more]
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BLAST of Surfeit locus protein 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Surfeit locus protein 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Surfeit locus protein 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO433936.270e-1031.53Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Surfeit locus protein 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
surf28.628e-1026.45surfeit 2 [Source:NCBI gene;Acc:101158543][more]
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BLAST of Surfeit locus protein 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000073153.17.394e-12999.49SMESG000073153.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035347 ID=SMED30035347|Name=Surfeit locus protein 2|organism=Schmidtea mediterranea sexual|type=transcript|length=642bp
CTTTGTTTAAATATTCAAAGCACGTTAAAATGAATGAAGAAATTAGAAAG
TTAATTGATTCCAATGAACACTTGGAATTTATTGAAGATTTAAATAAAGT
TAAATGTACTCTTACAGGACATGAAATGATAATGAATAAAGATATTATTG
ACAAGTACTTAAAGGGTAAAAAATTTAATTGTGCAAGAACTGAACATATA
TTCAAACAATATGATAAATTCTTGCGAAAGACTAAAGATAATGAAGTATA
TTGTGAGTTAACGTGCAAATTTATTAAATTCACATATAAAGACATTTATA
ATCATATAAATGGATACAATTTTTTAAGAGCATGTGAAAAATGGTTTAAA
TGTAAAGAATCAAAAAGTGAATACACACCAGTAACTACTGTTAGAAAGAA
GGATTTGGGAAAATACATGTCAATGATGAAGGAGTTAAACGGTGAAAATG
GCGAAGAATCAGAAAAGGATAATGGAGAAATTGAAACACCCAAAGAAGTT
TTATCAGAAAGTGAATCTGTTGAAGACAACGAAGAAAGGATGGAAGCAGA
TGATTCATCCGAGAAATCTAATGAAGATGAATCCGAACAAGAAGTTCCTA
TTAAAAAACGGAAAATGATTAATTAATAAATTTTCTCGAATG
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protein sequence of SMED30035347-orf-1

>SMED30035347-orf-1 ID=SMED30035347-orf-1|Name=SMED30035347-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=208bp
LFKYSKHVKMNEEIRKLIDSNEHLEFIEDLNKVKCTLTGHEMIMNKDIID
KYLKGKKFNCARTEHIFKQYDKFLRKTKDNEVYCELTCKFIKFTYKDIYN
HINGYNFLRACEKWFKCKESKSEYTPVTTVRKKDLGKYMSMMKELNGENG
EESEKDNGEIETPKEVLSESESVEDNEERMEADDSSEKSNEDESEQEVPI
KKRKMIN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005654nucleoplasm
GO:0005634nucleus
GO:0005730nucleolus
GO:0005886plasma membrane
GO:0016607nuclear speck
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR008833Surf2
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF05477SURF2coord: 12..195
e-value: 1.3E-18
score: 67.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 146..207
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 147..170
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..207
NoneNo IPR availablePANTHERPTHR34348SURFEIT LOCUS PROTEIN 2coord: 10..196