Phosphoglycerate kinase

Overview


Name	Phosphoglycerate kinase
Smed ID	SMED30035317
Length (bp)	1402

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Phosphoglycerate kinase (SMED30035317) t-SNE clustered cells

Violin plots show distribution of expression levels for Phosphoglycerate kinase (SMED30035317) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Phosphoglycerate kinase (SMED30035317) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Phosphoglycerate kinase (SMED30035317) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30035317

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pharynx	SMED30035317	SMESG000039090.1	dd_Smed_v4_972_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30035317	SMESG000039090.1	dd_Smed_v4_972_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30035317	SMESG000039090.1	dd_Smed_v4_972_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30035317	SMESG000039090.1	dd_Smed_v4_972_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30035317	SMESG000039090.1	dd_Smed_v4_972_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30035317	SMESG000039090.1	dd_Smed_v4_972_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30035317	SMESG000039090.1	dd_Smed_v4_972_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30035317	SMESG000039090.1	OX_Smed_1.0.01902	ox_Smed_v2	PMID:24238224 Kao et al., 2013
epidermis	SMED30035317	SMESG000039090.1	dd_Smed_v4_972_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Phosphoglycerate kinase vs. Ensembl Human
Match: PGK1 (phosphoglycerate kinase 1 [Source:HGNC Symbol;Acc:HGNC:8896])

HSP 1 Score: 580.096 bits (1494), Expect = 0.000e+0
Identity = 295/417 (70.74%), Postives = 345/417 (82.73%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DKLD+  KRV++RVDFNVPMK+ +ITNNQR+ AA+P+IK+ L+  AKSVVLMSHLGRPDG P   KYSLEPVA E+  LLGKDV FL DCVG  V  AC NPA GS+ LLENLRFHVEEEGKG DASGNK+KA   +++ F   L+ LGDVYVNDAFGTAHRAH+SMVG +LPQKA GFL+K EL YFAKALE+P RPFLAI+GGAKV+DKI LI NML+ V+E+II GGMAFTFLKVLNNMEIG SLFDE G+KIV+++++KA++  VKI LPVDFVT DKFDE+AK G AT+A+G+P G MGLDCG E+ K++  A+ RAK IVWNGP+GVFE+E F  GTKA MD VV  T  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI 417

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Human
Match: PGK2 (phosphoglycerate kinase 2 [Source:HGNC Symbol;Acc:HGNC:8898])

HSP 1 Score: 564.303 bits (1453), Expect = 0.000e+0
Identity = 288/417 (69.06%), Postives = 340/417 (81.53%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L KKL++DKLD+  KRV++RVDFNVPMK  +ITNNQR+ A++P+IKY L+  AK+VVLMSHLGRPDG P   KYSL PVA E+  LLGKDV FL DCVG  V  AC NPA GS+ LLENLRFHVEEEGKG D SG KIKA  ++++ F   L+ LGDVYVNDAFGTAHRAH+SMVG +LP KASGFL+K EL YFAKALENP RPFLAI+GGAKV+DKI LIKNML+ V+E+II GGMA+TFLKVLNNMEIG SLFDE G+KIV++I+ KA++  V+I  PVDFVTGDKFDE+A+VG AT+A+G+  G MGLDCG E+ K     + +A+ IVWNGP+GVFE++ F  GTKA MD +V  T  G  T+IGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGKILPGV ALS++
Sbjct:    1 MSLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASIPSIKYCLDNGAKAVVLMSHLGRPDGVPMPDKYSLAPVAVELKSLLGKDVLFLKDCVGAEVEKACANPAPGSVILLENLRFHVEEEGKGQDPSGKKIKAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPHKASGFLMKKELDYFAKALENPVRPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGMAYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIVKATSKGCITVIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKILPGVEALSNM 417

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Human
Match: PGK1 (phosphoglycerate kinase 1 [Source:HGNC Symbol;Acc:HGNC:8896])

HSP 1 Score: 541.576 bits (1394), Expect = 0.000e+0
Identity = 277/389 (71.21%), Postives = 321/389 (82.52%), Query Frame = 1

Query:  145 MKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            MK+ +ITNNQR+ AA+P+IK+ L+  AKSVVLMSHLGRPDG P   KYSLEPVA E+  LLGKDV FL DCVG  V  AC NPA GS+ LLENLRFHVEEEGKG DASGNK+KA   +++ F   L+ LGDVYVNDAFGTAHRAH+SMVG +LPQKA GFL+K EL YFAKALE+P RPFLAI+GGAKV+DKI LI NML+ V+E+II GGMAFTFLKVLNNMEIG SLFDE G+KIV+++++KA++  VKI LPVDFVT DKFDE+AK G AT+A+G+P G MGLDCG E+ K++  A+ RAK IVWNGP+GVFE+E F  GTKA MD VV  T  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI 389

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Celegans
Match: pgk-1 (Probable phosphoglycerate kinase [Source:UniProtKB/Swiss-Prot;Acc:P91427])

HSP 1 Score: 548.51 bits (1412), Expect = 0.000e+0
Identity = 284/409 (69.44%), Postives = 329/409 (80.44%), Query Frame = 1

Query:   76 KLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 1302
            KL++D+L+LA KRVLIRVDFNVP+KDGKITNNQR+ AA+PTI++AL   AKSVVLMSHLGRPDG    KY+L+PVA E+  LL KDV FL DCVG  V +AC +PA GS+ LLENLR+H+EEEGKGVDASG K+KA +  VKKF E LT LGD+YVNDAFGTAHRAH+SMVG    Q+ASGFLLK EL+YF+KAL+NPARPFLAI+GGAKV+DKI LIKN+L+ V+E+II GGMA+TFLKV   ++IGNSL+DE G+KIV E++  AK K V+IHLPVDFV  DKF E A     T   GVP G MGLD G E+ K F +AI RAKTIVWNGP GVFEF+ F  GTK+ MD VV  T AGA TIIGGGDTAT   K+ TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct:    6 KLAIDQLNLAGKRVLIRVDFNVPLKDGKITNNQRIAAAVPTIQHALSNGAKSVVLMSHLGRPDGRRQDKYTLKPVAEELKALLKKDVLFLDDCVGSEVEAACADPAPGSVILLENLRYHLEEEGKGVDASGAKVKADSAAVKKFRESLTKLGDIYVNDAFGTAHRAHSSMVGVEHSQRASGFLLKNELSYFSKALDNPARPFLAILGGAKVADKIQLIKNLLDKVNEMIIGGGMAYTFLKVAQGVKIGNSLYDEEGAKIVNELLEAAKAKGVQIHLPVDFVIADKFAEDATSKTVTAEEGVPDGHMGLDVGPESSKIFAAAIQRAKTIVWNGPAGVFEFDKFATGTKSLMDEVVKATAAGAITIIGGGDTATAAKKYNTEDKVSHVSTGGGASLELLEGKVLPGVDALS 414

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Fly
Match: Pgk (gene:FBgn0250906 transcript:FBtr0077739)

HSP 1 Score: 544.658 bits (1402), Expect = 0.000e+0
Identity = 278/409 (67.97%), Postives = 332/409 (81.17%), Query Frame = 1

Query:   76 KLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 1302
            KLS++ LDLA KRVL+RVDFNVP+K+GKIT+NQR+VAA+ +IK AL K AKSVVLMSHLGRPDG  N KY+L PVAAE+  LLG+DV FL DCVG  V +ACK+PA GS+ LLEN+RF+VEEEGKG+DASG K+KA   +VK+F   L  LGDVYVNDAFGTAHRAH+SM+G    Q+A+G LL  EL YF++AL+ P  PFLAI+GGAKV+DKI LI+N+L+ V+E+II GGMAFTFLKVLNNM+IG SLFDE GSKIV++++ KAK+ NV++HLPVDFV GDKF E+A V  AT+  G+P G MGLD G +T + F + I RAK IVWNGP GVFEF NF +GTK+ MD VV  TK G  +IIGGGDTA+CCAKW TE  VSHVSTGGGASLELLEGK LPGVAAL+
Sbjct:    5 KLSIENLDLAGKRVLMRVDFNVPIKEGKITSNQRIVAALDSIKLALSKKAKSVVLMSHLGRPDGNKNIKYTLAPVAAELKTLLGQDVIFLSDCVGSEVEAACKDPAPGSVILLENVRFYVEEEGKGLDASGGKVKADPAKVKEFRASLAKLGDVYVNDAFGTAHRAHSSMMGDGFEQRAAGLLLNKELKYFSQALDKPPNPFLAILGGAKVADKIQLIENLLDKVNEMIIGGGMAFTFLKVLNNMKIGGSLFDEEGSKIVEKLVEKAKKNNVQLHLPVDFVCGDKFAENAAVSEATVEAGIPDGHMGLDVGPKTRELFAAPIARAKLIVWNGPPGVFEFPNFANGTKSIMDGVVAATKNGTVSIIGGGDTASCCAKWNTEALVSHVSTGGGASLELLEGKTLPGVAALT 413

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Fly
Match: Pgk (gene:FBgn0250906 transcript:FBtr0330718)

HSP 1 Score: 544.658 bits (1402), Expect = 0.000e+0
Identity = 278/409 (67.97%), Postives = 332/409 (81.17%), Query Frame = 1

Query:   76 KLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 1302
            KLS++ LDLA KRVL+RVDFNVP+K+GKIT+NQR+VAA+ +IK AL K AKSVVLMSHLGRPDG  N KY+L PVAAE+  LLG+DV FL DCVG  V +ACK+PA GS+ LLEN+RF+VEEEGKG+DASG K+KA   +VK+F   L  LGDVYVNDAFGTAHRAH+SM+G    Q+A+G LL  EL YF++AL+ P  PFLAI+GGAKV+DKI LI+N+L+ V+E+II GGMAFTFLKVLNNM+IG SLFDE GSKIV++++ KAK+ NV++HLPVDFV GDKF E+A V  AT+  G+P G MGLD G +T + F + I RAK IVWNGP GVFEF NF +GTK+ MD VV  TK G  +IIGGGDTA+CCAKW TE  VSHVSTGGGASLELLEGK LPGVAAL+
Sbjct:    5 KLSIENLDLAGKRVLMRVDFNVPIKEGKITSNQRIVAALDSIKLALSKKAKSVVLMSHLGRPDGNKNIKYTLAPVAAELKTLLGQDVIFLSDCVGSEVEAACKDPAPGSVILLENVRFYVEEEGKGLDASGGKVKADPAKVKEFRASLAKLGDVYVNDAFGTAHRAHSSMMGDGFEQRAAGLLLNKELKYFSQALDKPPNPFLAILGGAKVADKIQLIENLLDKVNEMIIGGGMAFTFLKVLNNMKIGGSLFDEEGSKIVEKLVEKAKKNNVQLHLPVDFVCGDKFAENAAVSEATVEAGIPDGHMGLDVGPKTRELFAAPIARAKLIVWNGPPGVFEFPNFANGTKSIMDGVVAATKNGTVSIIGGGDTASCCAKWNTEALVSHVSTGGGASLELLEGKTLPGVAALT 413

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Fly
Match: Pgk (gene:FBgn0250906 transcript:FBtr0330716)

HSP 1 Score: 544.658 bits (1402), Expect = 0.000e+0
Identity = 278/409 (67.97%), Postives = 332/409 (81.17%), Query Frame = 1

Query:   76 KLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 1302
            KLS++ LDLA KRVL+RVDFNVP+K+GKIT+NQR+VAA+ +IK AL K AKSVVLMSHLGRPDG  N KY+L PVAAE+  LLG+DV FL DCVG  V +ACK+PA GS+ LLEN+RF+VEEEGKG+DASG K+KA   +VK+F   L  LGDVYVNDAFGTAHRAH+SM+G    Q+A+G LL  EL YF++AL+ P  PFLAI+GGAKV+DKI LI+N+L+ V+E+II GGMAFTFLKVLNNM+IG SLFDE GSKIV++++ KAK+ NV++HLPVDFV GDKF E+A V  AT+  G+P G MGLD G +T + F + I RAK IVWNGP GVFEF NF +GTK+ MD VV  TK G  +IIGGGDTA+CCAKW TE  VSHVSTGGGASLELLEGK LPGVAAL+
Sbjct:    5 KLSIENLDLAGKRVLMRVDFNVPIKEGKITSNQRIVAALDSIKLALSKKAKSVVLMSHLGRPDGNKNIKYTLAPVAAELKTLLGQDVIFLSDCVGSEVEAACKDPAPGSVILLENVRFYVEEEGKGLDASGGKVKADPAKVKEFRASLAKLGDVYVNDAFGTAHRAHSSMMGDGFEQRAAGLLLNKELKYFSQALDKPPNPFLAILGGAKVADKIQLIENLLDKVNEMIIGGGMAFTFLKVLNNMKIGGSLFDEEGSKIVEKLVEKAKKNNVQLHLPVDFVCGDKFAENAAVSEATVEAGIPDGHMGLDVGPKTRELFAAPIARAKLIVWNGPPGVFEFPNFANGTKSIMDGVVAATKNGTVSIIGGGDTASCCAKWNTEALVSHVSTGGGASLELLEGKTLPGVAALT 413

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Fly
Match: Pgk (gene:FBgn0250906 transcript:FBtr0334162)

HSP 1 Score: 544.658 bits (1402), Expect = 0.000e+0
Identity = 278/409 (67.97%), Postives = 332/409 (81.17%), Query Frame = 1

Query:   76 KLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 1302
            KLS++ LDLA KRVL+RVDFNVP+K+GKIT+NQR+VAA+ +IK AL K AKSVVLMSHLGRPDG  N KY+L PVAAE+  LLG+DV FL DCVG  V +ACK+PA GS+ LLEN+RF+VEEEGKG+DASG K+KA   +VK+F   L  LGDVYVNDAFGTAHRAH+SM+G    Q+A+G LL  EL YF++AL+ P  PFLAI+GGAKV+DKI LI+N+L+ V+E+II GGMAFTFLKVLNNM+IG SLFDE GSKIV++++ KAK+ NV++HLPVDFV GDKF E+A V  AT+  G+P G MGLD G +T + F + I RAK IVWNGP GVFEF NF +GTK+ MD VV  TK G  +IIGGGDTA+CCAKW TE  VSHVSTGGGASLELLEGK LPGVAAL+
Sbjct:    5 KLSIENLDLAGKRVLMRVDFNVPIKEGKITSNQRIVAALDSIKLALSKKAKSVVLMSHLGRPDGNKNIKYTLAPVAAELKTLLGQDVIFLSDCVGSEVEAACKDPAPGSVILLENVRFYVEEEGKGLDASGGKVKADPAKVKEFRASLAKLGDVYVNDAFGTAHRAHSSMMGDGFEQRAAGLLLNKELKYFSQALDKPPNPFLAILGGAKVADKIQLIENLLDKVNEMIIGGGMAFTFLKVLNNMKIGGSLFDEEGSKIVEKLVEKAKKNNVQLHLPVDFVCGDKFAENAAVSEATVEAGIPDGHMGLDVGPKTRELFAAPIARAKLIVWNGPPGVFEFPNFANGTKSIMDGVVAATKNGTVSIIGGGDTASCCAKWNTEALVSHVSTGGGASLELLEGKTLPGVAALT 413

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Fly
Match: Pgk (gene:FBgn0250906 transcript:FBtr0330717)

HSP 1 Score: 544.658 bits (1402), Expect = 0.000e+0
Identity = 278/409 (67.97%), Postives = 332/409 (81.17%), Query Frame = 1

Query:   76 KLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 1302
            KLS++ LDLA KRVL+RVDFNVP+K+GKIT+NQR+VAA+ +IK AL K AKSVVLMSHLGRPDG  N KY+L PVAAE+  LLG+DV FL DCVG  V +ACK+PA GS+ LLEN+RF+VEEEGKG+DASG K+KA   +VK+F   L  LGDVYVNDAFGTAHRAH+SM+G    Q+A+G LL  EL YF++AL+ P  PFLAI+GGAKV+DKI LI+N+L+ V+E+II GGMAFTFLKVLNNM+IG SLFDE GSKIV++++ KAK+ NV++HLPVDFV GDKF E+A V  AT+  G+P G MGLD G +T + F + I RAK IVWNGP GVFEF NF +GTK+ MD VV  TK G  +IIGGGDTA+CCAKW TE  VSHVSTGGGASLELLEGK LPGVAAL+
Sbjct:    5 KLSIENLDLAGKRVLMRVDFNVPIKEGKITSNQRIVAALDSIKLALSKKAKSVVLMSHLGRPDGNKNIKYTLAPVAAELKTLLGQDVIFLSDCVGSEVEAACKDPAPGSVILLENVRFYVEEEGKGLDASGGKVKADPAKVKEFRASLAKLGDVYVNDAFGTAHRAHSSMMGDGFEQRAAGLLLNKELKYFSQALDKPPNPFLAILGGAKVADKIQLIENLLDKVNEMIIGGGMAFTFLKVLNNMKIGGSLFDEEGSKIVEKLVEKAKKNNVQLHLPVDFVCGDKFAENAAVSEATVEAGIPDGHMGLDVGPKTRELFAAPIARAKLIVWNGPPGVFEFPNFANGTKSIMDGVVAATKNGTVSIIGGGDTASCCAKWNTEALVSHVSTGGGASLELLEGKTLPGVAALT 413

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BLAST of Phosphoglycerate kinase vs. Ensembl Zebrafish
Match: pgk1 (phosphoglycerate kinase 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2711])

HSP 1 Score: 561.222 bits (1445), Expect = 0.000e+0
Identity = 289/417 (69.30%), Postives = 336/417 (80.58%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL +DK+D+  KRV++RVDFNVPMK+  ITNNQR+ AA+P+I++ L+  AK+VVLMSHLGRPDG P   KYSLEPVAAE+  LLGKDV+FL DCVG  V  AC +P  GS+ LLENLRFHV EEGKG DASGNK KAS  E+  F   L+ LGDVYVNDAFGTAHRAH+SMVG +LPQKA+GFL+K EL YFA ALE P RPFLAI+GGAKV DKI LI NML+ V+E+II GGMAFTFLKVL NMEIG SLFDE GS IV++++ KA++  VKI LPVDF+T DKFDE A  G AT+A G+P G MGLDCG E+ K +  A+ RAK IVWNGP+GVFE++NF HGTK  MD VV+ TK G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLHLDKVDVKGKRVIMRVDFNVPMKNNVITNNQRIKAAVPSIQHCLDNCAKAVVLMSHLGRPDGVPMPDKYSLEPVAAELKNLLGKDVQFLKDCVGPEVEKACADPPAGSVILLENLRFHVAEEGKGKDASGNKTKASQAEIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAAGFLMKKELDYFAMALEKPQRPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLKNMEIGTSLFDEEGSTIVKDLMAKAEKNGVKITLPVDFITADKFDEKATTGTATVAEGIPAGWMGLDCGPESSKLYAEAVARAKQIVWNGPVGVFEWDNFAHGTKNMMDKVVEATKNGCITIIGGGDTATCCAKWDTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV 417

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Xenopus
Match: mfsd6l (major facilitator superfamily domain containing 6-like [Source:Xenbase;Acc:XB-GENE-5841491])

HSP 1 Score: 562.377 bits (1448), Expect = 0.000e+0
Identity = 284/417 (68.11%), Postives = 344/417 (82.49%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DK+D+  KRV++RVDFNVPMK+ +ITNNQR+ AA+P+I++ L+  AKSVVLMSHLGRPDG P   KYSL PVA E+  L+ +++ FL DCVG  V +AC +PA G++ LLENLRFHVEEEGKG DA+GNK+KA A +V  F   L+ LGDVY+NDAFGTAHRAH+SMVG  LPQ+A+GFL+K EL YFAKALENP RPFLAI+GGAKV DKI LI NML+ V+E+II GGMAFTFLKVLNNM IGNSL+DE G+KIV++++ KA++  VKI LPVDF T +KFDE+A  GHA+++TG+P G MGLDCG E+MK F  A+ RAK IVWNGP+GVFE++NF  GTKA MD VV+VT  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLTLDKVDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIQHCLDHGAKSVVLMSHLGRPDGVPMPDKYSLAPVAEELKALMKREIVFLKDCVGPEVEAACADPAPGTVLLLENLRFHVEEEGKGKDAAGNKVKADAAKVDAFRASLSKLGDVYINDAFGTAHRAHSSMVGVKLPQRAAGFLMKKELDYFAKALENPERPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMSIGNSLYDEEGAKIVKDLMAKAEKNGVKITLPVDFTTAEKFDENAGTGHASVSTGIPDGWMGLDCGPESMKLFVEAVGRAKQIVWNGPVGVFEWDNFAKGTKAVMDKVVEVTGKGCITIIGGGDTATCCAKWDTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV 417

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Xenopus
Match: mfsd6l (major facilitator superfamily domain containing 6-like [Source:Xenbase;Acc:XB-GENE-5841491])

HSP 1 Score: 539.65 bits (1389), Expect = 0.000e+0
Identity = 273/398 (68.59%), Postives = 328/398 (82.41%), Query Frame = 1

Query:  118 LIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            ++ VDFNVPMK+ +ITNNQR+ AA+P+I++ L+  AKSVVLMSHLGRPDG P   KYSL PVA E+  L+ +++ FL DCVG  V +AC +PA G++ LLENLRFHVEEEGKG DA+GNK+KA A +V  F   L+ LGDVY+NDAFGTAHRAH+SMVG  LPQ+A+GFL+K EL YFAKALENP RPFLAI+GGAKV DKI LI NML+ V+E+II GGMAFTFLKVLNNM IGNSL+DE G+KIV++++ KA++  VKI LPVDF T +KFDE+A  GHA+++TG+P G MGLDCG E+MK F  A+ RAK IVWNGP+GVFE++NF  GTKA MD VV+VT  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:   21 MVMVDFNVPMKNNQITNNQRIKAAVPSIQHCLDHGAKSVVLMSHLGRPDGVPMPDKYSLAPVAEELKALMKREIVFLKDCVGPEVEAACADPAPGTVLLLENLRFHVEEEGKGKDAAGNKVKADAAKVDAFRASLSKLGDVYINDAFGTAHRAHSSMVGVKLPQRAAGFLMKKELDYFAKALENPERPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMSIGNSLYDEEGAKIVKDLMAKAEKNGVKITLPVDFTTAEKFDENAGTGHASVSTGIPDGWMGLDCGPESMKLFVEAVGRAKQIVWNGPVGVFEWDNFAKGTKAVMDKVVEVTGKGCITIIGGGDTATCCAKWDTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV 418

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Xenopus
Match: mfsd6l (major facilitator superfamily domain containing 6-like [Source:Xenbase;Acc:XB-GENE-5841491])

HSP 1 Score: 521.161 bits (1341), Expect = 0.000e+0
Identity = 270/417 (64.75%), Postives = 328/417 (78.66%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DK+D+  KRV++R+                  AA+P+I++ L+  AKSVVLMSHLGRPDG P   KYSL PVA E+  L+ +++ FL DCVG  V +AC +PA G++ LLENLRFHVEEEGKG DA+GNK+KA A +V  F   L+ LGDVY+NDAFGTAHRAH+SMVG  LPQ+A+GFL+K EL YFAKALENP RPFLAI+GGAKV DKI LI NML+ V+E+II GGMAFTFLKVLNNM IGNSL+DE G+KIV++++ KA++  VKI LPVDF T +KFDE+A  GHA+++TG+P G MGLDCG E+MK F  A+ RAK IVWNGP+GVFE++NF  GTKA MD VV+VT  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLTLDKVDVKGKRVVMRIK-----------------AAVPSIQHCLDHGAKSVVLMSHLGRPDGVPMPDKYSLAPVAEELKALMKREIVFLKDCVGPEVEAACADPAPGTVLLLENLRFHVEEEGKGKDAAGNKVKADAAKVDAFRASLSKLGDVYINDAFGTAHRAHSSMVGVKLPQRAAGFLMKKELDYFAKALENPERPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMSIGNSLYDEEGAKIVKDLMAKAEKNGVKITLPVDFTTAEKFDENAGTGHASVSTGIPDGWMGLDCGPESMKLFVEAVGRAKQIVWNGPVGVFEWDNFAKGTKAVMDKVVEVTGKGCITIIGGGDTATCCAKWDTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV 400

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Xenopus
Match: mfsd6l (major facilitator superfamily domain containing 6-like [Source:Xenbase;Acc:XB-GENE-5841491])

HSP 1 Score: 520.005 bits (1338), Expect = 0.000e+0
Identity = 270/417 (64.75%), Postives = 329/417 (78.90%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DK+D+  KRV++R+   +              AA+P+I++ L+  AKSVVLMSHLGRPDG P   KYSL PVA E+  L+ +++ FL DCVG  V +AC +PA G++ LLENLRFHVEEEGKG DA+GNK+KA A +V  F   L+ LGDVY+NDAFGTAHRAH+SMVG  LPQ+A+GFL+K EL YFAKALENP RPFLAI+GGAKV DKI LI NML+ V+E+II GGMAFTFLKVLNNM IGNSL+DE G+KIV++++ KA++  VKI LPVDF T +KFDE+A  GHA+++TG+P G MGLDCG E+MK F  A+ RAK IVWNGP+GVFE++NF  GTKA MD VV+VT  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLTLDKVDVKGKRVVMRLILLIK-------------AAVPSIQHCLDHGAKSVVLMSHLGRPDGVPMPDKYSLAPVAEELKALMKREIVFLKDCVGPEVEAACADPAPGTVLLLENLRFHVEEEGKGKDAAGNKVKADAAKVDAFRASLSKLGDVYINDAFGTAHRAHSSMVGVKLPQRAAGFLMKKELDYFAKALENPERPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMSIGNSLYDEEGAKIVKDLMAKAEKNGVKITLPVDFTTAEKFDENAGTGHASVSTGIPDGWMGLDCGPESMKLFVEAVGRAKQIVWNGPVGVFEWDNFAKGTKAVMDKVVEVTGKGCITIIGGGDTATCCAKWDTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV 404

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Xenopus
Match: mfsd6l (major facilitator superfamily domain containing 6-like [Source:Xenbase;Acc:XB-GENE-5841491])

HSP 1 Score: 506.908 bits (1304), Expect = 1.875e-178
Identity = 267/419 (63.72%), Postives = 327/419 (78.04%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHA--SMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DK+D+  KRV++RVDFNVPMK+ +ITNNQR+ AA+P+I++ L+  AKSVVLMSHLGRPDG P   KYSL PVA E+  L+ +++ FL DCVG  V +AC +PA G++ LLENLRFHVEEEGKG DA+GNK+              +   DV ++     AHR  +  SMVG  LPQ+A+GFL+K EL YFAKALENP RPFLAI+GGAKV DKI LI NML+ V+E+II GGMAFTFLKVLNNM IGNSL+DE G+KIV++++ KA++  VKI LPVDF T +KFDE+A  GHA+++TG+P G MGLDCG E+MK F  A+ RAK IVWNGP+GVFE++NF  GTKA MD VV+VT  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLTLDKVDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIQHCLDHGAKSVVLMSHLGRPDGVPMPDKYSLAPVAEELKALMKREIVFLKDCVGPEVEAACADPAPGTVLLLENLRFHVEEEGKGKDAAGNKV--------------SLFTDVKLHLLRAVAHRETSCLSMVGVKLPQRAAGFLMKKELDYFAKALENPERPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMSIGNSLYDEEGAKIVKDLMAKAEKNGVKITLPVDFTTAEKFDENAGTGHASVSTGIPDGWMGLDCGPESMKLFVEAVGRAKQIVWNGPVGVFEWDNFAKGTKAVMDKVVEVTGKGCITIIGGGDTATCCAKWDTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV 405

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Mouse
Match: Pgk1 (phosphoglycerate kinase 1 [Source:MGI Symbol;Acc:MGI:97555])

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 293/417 (70.26%), Postives = 346/417 (82.97%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DKLD+  KRV++RVDFNVPMK+ +ITNNQR+ AA+P+IK+ L+  AKSVVLMSHLGRPDG P   KYSLEPVAAE+  LLGKDV FL DCVG  V +AC NPA G++ LLENLRFHVEEEGKG DASGNK+KA   ++  F   L+ LGDVYVNDAFGTAHRAH+SMVG +LPQKA GFL+K EL YFAKALE+P RPFLAI+GGAKV+DKI LI NML+ V+E+II GGMAFTFLKVLNNMEIG SL+DE G+KIV+++++KA++  VKI LPVDFVT DKFDE+AK G AT+A+G+P G MGLDCG E+ K++  A+ RAK IVWNGP+GVFE+E F  GTK+ MD VV  T  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAAELKSLLGKDVLFLKDCVGPEVENACANPAAGTVILLENLRFHVEEEGKGKDASGNKVKAEPAKIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLYDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGTESSKKYAEAVGRAKQIVWNGPVGVFEWEAFARGTKSLMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV 417

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Mouse
Match: Pgk2 (phosphoglycerate kinase 2 [Source:MGI Symbol;Acc:MGI:97563])

HSP 1 Score: 551.977 bits (1421), Expect = 0.000e+0
Identity = 288/417 (69.06%), Postives = 334/417 (80.10%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M L  KL++DK+DL  KRV++RVDFNVPMK+ +ITNNQR+ AA+P+IK+ L+  AKSVVLMSHLGRPDG P   KYSLEPVA E+  LL KDV FL DCVG  V  AC NP +GSI LLENLRFHVEEEGKG D+SG KI A   +V+ F   L+ LGDVYVNDAFGTAHRAH+S VG +LPQKASGFL+K EL YF+KALE P RPFLAI+GGAKV DKI LIKNML+ V+ +II GGMA+TFLK L NM+IG SLFDE G+ IV+EI+ KA++  VKI  PVDFVTGDKFDE+AKVG AT+ +G+P G MGLDCG E++K     + +AK IVWNGP+GVFE++ F  GTKA MD VV  T  G  TIIGGGDTATCCAKWGTEDKVSHVSTGGGASLELLEGKILPGV ALS++
Sbjct:    1 MALSAKLTLDKVDLKGKRVIMRVDFNVPMKNNQITNNQRIKAAIPSIKHCLDNGAKSVVLMSHLGRPDGIPMPDKYSLEPVADELKSLLNKDVIFLKDCVGPEVEQACANPDNGSIILLENLRFHVEEEGKGKDSSGKKISADPAKVEAFQASLSKLGDVYVNDAFGTAHRAHSSTVGVNLPQKASGFLMKKELDYFSKALEKPERPFLAILGGAKVKDKIQLIKNMLDKVNFMIIGGGMAYTFLKELKNMQIGASLFDEEGATIVKEIMEKAEKNGVKIVFPVDFVTGDKFDENAKVGQATIESGIPSGWMGLDCGPESIKINAQIVAQAKLIVWNGPIGVFEWDAFAKGTKALMDEVVKATSNGCVTIIGGGDTATCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVEALSNM 417

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Mouse
Match: Pgk1 (phosphoglycerate kinase 1 [Source:MGI Symbol;Acc:MGI:97555])

HSP 1 Score: 366.696 bits (940), Expect = 9.013e-126
Identity = 180/252 (71.43%), Postives = 210/252 (83.33%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNME 813
            M+L  KL++DKLD+  KRV++RVDFNVPMK+ +ITNNQR+ AA+P+IK+ L+  AKSVVLMSHLGRPDG P   KYSLEPVAAE+  LLGKDV FL DCVG  V +AC NPA G++ LLENLRFHVEEEGKG DASGNK+KA   ++  F   L+ LGDVYVNDAFGTAHRAH+SMVG +LPQKA GFL+K EL YFAKALE+P RPFLAI+GGAKV+DKI LI NML+ V+E+II GGMAFTFLKVLNNME
Sbjct:    1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAAELKSLLGKDVLFLKDCVGPEVENACANPAAGTVILLENLRFHVEEEGKGKDASGNKVKAEPAKIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNME 252

[Close]

BLAST of Phosphoglycerate kinase vs. UniProt/SwissProt
Match: sp|P41759|PGK_SCHMA (Phosphoglycerate kinase OS=Schistosoma mansoni OX=6183 GN=PGK PE=1 SV=1)

HSP 1 Score: 580.867 bits (1496), Expect = 0.000e+0
Identity = 300/410 (73.17%), Postives = 338/410 (82.44%), Query Frame = 1

Query:   76 KLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSD 1305
            KLS+  +DL  KRVLIRVDFNVPMKDGK+TN QR+ AA+PTIKYAL+K AKSVVLMSHLGRPDG    KYSL+PV  EVSKLLGK+V FL+DCVG  V++AC NPA GS+FLLENLRFHVEEEGKGV  +G K KA+A+++K FSE LT LGDVYVNDAFGTAHRAHASMVGC LPQKA GFL+  ELTYFAKALENP RPFLAI+GGAKVSDKI LI NML+ V+ELII GGMA+TFLK ++NM IGNSLFD  G++IV +++  AK KNV IHLPVDFVT DKF + A     T+ +G+  G MGLD G +T++EF   I RAKTIVWNGPMGVFE + F  GTKAAMD VV  TK GATTIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK LPGV AL+D
Sbjct:    5 KLSISDVDLKGKRVLIRVDFNVPMKDGKVTNTQRIAAAIPTIKYALDKGAKSVVLMSHLGRPDGHKVDKYSLKPVCPEVSKLLGKEVTFLNDCVGPDVVNACANPAPGSVFLLENLRFHVEEEGKGVSPTGEKTKATADQIKAFSESLTKLGDVYVNDAFGTAHRAHASMVGCQLPQKACGFLMNKELTYFAKALENPERPFLAILGGAKVSDKIQLINNMLDKVNELIIGGGMAYTFLKQIHNMHIGNSLFDAPGAEIVHKVMETAKAKNVAIHLPVDFVTADKFADDANTEIRTIQSGIADGWMGLDIGPKTIEEFSKVISRAKTIVWNGPMGVFEMDKFATGTKAAMDEVVKATKNGATTIIGGGDTATCCAKWDTEDKVSHVSTGGGASLELLEGKQLPGVVALTD 414

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BLAST of Phosphoglycerate kinase vs. UniProt/SwissProt
Match: sp|Q5NVB5|PGK1_PONAB (Phosphoglycerate kinase 1 OS=Pongo abelii OX=9601 GN=PGK1 PE=2 SV=4)

HSP 1 Score: 580.867 bits (1496), Expect = 0.000e+0
Identity = 295/417 (70.74%), Postives = 345/417 (82.73%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DKLD+  KRV++RVDFNVPMK+ +ITNNQR+ AA+P+IK+ L+  AKSVVLMSHLGRPDG P   KYSLEPVA E+  LLGKDV FL DCVG  V  AC NPA GS+ LLENLRFHVEEEGKG DASGNK+KA   +++ F   L+ LGDVYVNDAFGTAHRAH+SMVG +LPQKA GFL+K EL YFAKALE+P RPFLAI+GGAKV+DKI LI NML+ V+E+II GGMAFTFLKVLNNMEIG SLFDE G+KIV+++++KA++  VKI LPVDFVT DKFDE+AK G AT+A+G+P G MGLDCG E+ K++  A+ RAK IVWNGP+GVFE+E F  GTKA MD VV  T  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFAQGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI 417

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BLAST of Phosphoglycerate kinase vs. UniProt/SwissProt
Match: sp|Q60HD8|PGK1_MACFA (Phosphoglycerate kinase 1 OS=Macaca fascicularis OX=9541 GN=PGK1 PE=2 SV=3)

HSP 1 Score: 580.867 bits (1496), Expect = 0.000e+0
Identity = 295/417 (70.74%), Postives = 345/417 (82.73%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DKLD+  KRV++RVDFNVPMK+ +ITNNQR+ AA+P+IK+ L+  AKSVVLMSHLGRPDG P   KYSLEPVA E+  LLGKDV FL DCVG  V  AC NPA GS+ LLENLRFHVEEEGKG DASGNK+KA   +++ F   L+ LGDVYVNDAFGTAHRAH+SMVG +LPQKA GFL+K EL YFAKALE+P RPFLAI+GGAKV+DKI LI NML+ V+E+II GGMAFTFLKVLNNMEIG SLFDE G+KIV+++++KA++  VKI LPVDFVT DKFDE+AK G AT+A+G+P G MGLDCG E+ K++  A+ RAK IVWNGP+GVFE+E F  GTKA MD VV  T  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFAQGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI 417

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BLAST of Phosphoglycerate kinase vs. UniProt/SwissProt
Match: sp|A5A6K4|PGK1_PANTR (Phosphoglycerate kinase 1 OS=Pan troglodytes OX=9598 GN=PGK1 PE=2 SV=1)

HSP 1 Score: 580.096 bits (1494), Expect = 0.000e+0
Identity = 295/417 (70.74%), Postives = 345/417 (82.73%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DKLD+  KRV++RVDFNVPMK+ +ITNNQR+ AA+P+IK+ L+  AKSVVLMSHLGRPDG P   KYSLEPVA E+  LLGKDV FL DCVG  V  AC NPA GS+ LLENLRFHVEEEGKG DASGNK+KA   +++ F   L+ LGDVYVNDAFGTAHRAH+SMVG +LPQKA GFL+K EL YFAKALE+P RPFLAI+GGAKV+DKI LI NML+ V+E+II GGMAFTFLKVLNNMEIG SLFDE G+KIV+++++KA++  VKI LPVDFVT DKFDE+AK G AT+A+G+P G MGLDCG E+ K++  A+ RAK IVWNGP+GVFE+E F  GTKA MD VV  T  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI 417

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BLAST of Phosphoglycerate kinase vs. UniProt/SwissProt
Match: sp|P00558|PGK1_HUMAN (Phosphoglycerate kinase 1 OS=Homo sapiens OX=9606 GN=PGK1 PE=1 SV=3)

HSP 1 Score: 580.096 bits (1494), Expect = 0.000e+0
Identity = 295/417 (70.74%), Postives = 345/417 (82.73%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DKLD+  KRV++RVDFNVPMK+ +ITNNQR+ AA+P+IK+ L+  AKSVVLMSHLGRPDG P   KYSLEPVA E+  LLGKDV FL DCVG  V  AC NPA GS+ LLENLRFHVEEEGKG DASGNK+KA   +++ F   L+ LGDVYVNDAFGTAHRAH+SMVG +LPQKA GFL+K EL YFAKALE+P RPFLAI+GGAKV+DKI LI NML+ V+E+II GGMAFTFLKVLNNMEIG SLFDE G+KIV+++++KA++  VKI LPVDFVT DKFDE+AK G AT+A+G+P G MGLDCG E+ K++  A+ RAK IVWNGP+GVFE+E F  GTKA MD VV  T  G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS++
Sbjct:    1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI 417

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BLAST of Phosphoglycerate kinase vs. TrEMBL
Match: A0A183B9P9 (Phosphoglycerate kinase OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS15934 PE=3 SV=1)

HSP 1 Score: 612.838 bits (1579), Expect = 0.000e+0
Identity = 319/411 (77.62%), Postives = 349/411 (84.91%), Query Frame = 1

Query:   73 KKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSD 1305
            KKLS+  +DL  KRVLIRVDFNVPMKDGKITN QR+VAA+PTIKYALEK A SVVLMSHLGRPDG    KYSL+PVA EVSKLLGK V+FL DCVGDA I AC NPA GS+FLLENLRFHVEEEGKGV  +G K+KA   +VK FSE LT LGDVYVNDAFGTAHRAHASMVGC+LPQKA GFL+  ELTYFAKALENPARPFLAIIGGAKVSDKI LIKNML+ V+ELIITGGMAFTFLK L NM+IG SL+DE G+ IV E++  AK+KNV+IHLPVDFVTG KF + A VG AT+ +G+P   MGLDCG +TM++F + I RAKTIVWNGPMGVFE E F  GTKAAMDAVV+ TK GATTIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK LPGV ALSD
Sbjct:    4 KKLSIANVDLKGKRVLIRVDFNVPMKDGKITNTQRIVAALPTIKYALEKGATSVVLMSHLGRPDGNKVDKYSLKPVAEEVSKLLGKPVQFLPDCVGDATIQACSNPAPGSVFLLENLRFHVEEEGKGVGPNGEKVKADPAKVKAFSESLTKLGDVYVNDAFGTAHRAHASMVGCNLPQKACGFLMDKELTYFAKALENPARPFLAIIGGAKVSDKIQLIKNMLDKVNELIITGGMAFTFLKKLYNMKIGKSLYDEPGAAIVDEVMELAKQKNVEIHLPVDFVTGSKFGDDATVGSATVESGIPEDYMGLDCGPKTMEKFRAVILRAKTIVWNGPMGVFEMEKFAGGTKAAMDAVVEATKNGATTIIGGGDTATCCAKWKTEDKVSHVSTGGGASLELLEGKELPGVVALSD 414

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BLAST of Phosphoglycerate kinase vs. TrEMBL
Match: A0A504Z124 (Phosphoglycerate kinase OS=Fasciola gigantica OX=46835 GN=FGIG_04452 PE=3 SV=1)

HSP 1 Score: 610.142 bits (1572), Expect = 0.000e+0
Identity = 318/411 (77.37%), Postives = 351/411 (85.40%), Query Frame = 1

Query:   73 KKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSD 1305
            KKLS+  +DL  KRVLIRVDFNVPMKDGKITNNQR+VAA+PTIKYALEK AKSVVLMSHLGRPDG    KYSL+PVA EVSKLLGK V+FL DCVGDA + AC NPA GS+FLLENLRFHVEEEGKGV  +G K+KA   +VK FSE LT LGDVYVNDAFGTAHRAHASMVGC+LPQKA GFL+  ELTYFAKALENPARPFLAIIGGAKVSDKI LIKNML+ V+ELIITGGMAFTFLK L+NM+IG SLFDE G+ IV E++  AK+K V+IHLPVDFVTG KF + A VG AT+ +G+P   MGLDCG +TM++F + I RAKTIVWNGPMGVFE E F  GTKAAMDAVV+ TK GATTIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK LPGV AL++
Sbjct:    4 KKLSIANVDLKGKRVLIRVDFNVPMKDGKITNNQRIVAALPTIKYALEKGAKSVVLMSHLGRPDGNKVDKYSLKPVAEEVSKLLGKAVQFLPDCVGDATLQACSNPAPGSVFLLENLRFHVEEEGKGVGPNGEKVKADPAKVKAFSESLTKLGDVYVNDAFGTAHRAHASMVGCNLPQKACGFLMDKELTYFAKALENPARPFLAIIGGAKVSDKIQLIKNMLDKVNELIITGGMAFTFLKKLHNMKIGKSLFDEPGAAIVDEVMALAKQKKVEIHLPVDFVTGSKFGDDATVGSATVESGIPDDYMGLDCGPKTMEKFRAVILRAKTIVWNGPMGVFEMEKFAGGTKAAMDAVVEATKNGATTIIGGGDTATCCAKWKTEDKVSHVSTGGGASLELLEGKELPGVVALTN 414

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BLAST of Phosphoglycerate kinase vs. TrEMBL
Match: A0A4E0RCZ7 (Phosphoglycerate kinase OS=Fasciola hepatica OX=6192 GN=D915_002597 PE=3 SV=1)

HSP 1 Score: 610.142 bits (1572), Expect = 0.000e+0
Identity = 318/411 (77.37%), Postives = 351/411 (85.40%), Query Frame = 1

Query:   73 KKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSD 1305
            KKLS+  +DL  KRVLIRVDFNVPMKDGKITNNQR+VAA+PTIKYALEK AKSVVLMSHLGRPDG    KYSL+PVA EVSKLLGK V+FL DCVGDA + AC NPA GS+FLLENLRFHVEEEGKGV  +G K+KA   +VK FSE LT LGDVYVNDAFGTAHRAHASMVGC+LPQKA GFL+  ELTYFAKALENPARPFLAIIGGAKVSDKI LIKNML+ V+ELIITGGMAFTFLK L+NM+IG SLFDE G+ IV E++  AK+K V+IHLPVDFVTG KF + A VG AT+ +G+P   MGLDCG +TM++F + I RAKTIVWNGPMGVFE E F  GTKAAMDAVV+ TK GATTIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK LPGV AL++
Sbjct:    4 KKLSIANVDLKGKRVLIRVDFNVPMKDGKITNNQRIVAALPTIKYALEKGAKSVVLMSHLGRPDGNKVDKYSLKPVAEEVSKLLGKAVQFLPDCVGDATLQACSNPAPGSVFLLENLRFHVEEEGKGVGPNGEKVKADPAKVKAFSESLTKLGDVYVNDAFGTAHRAHASMVGCNLPQKACGFLMDKELTYFAKALENPARPFLAIIGGAKVSDKIQLIKNMLDKVNELIITGGMAFTFLKKLHNMKIGKSLFDEPGAAIVDEVMALAKQKKVEIHLPVDFVTGSKFGDDATVGSATVESGIPDDYMGLDCGPKTMEKFRAVILRAKTIVWNGPMGVFEMEKFAGGTKAAMDAVVEATKNGATTIIGGGDTATCCAKWKTEDKVSHVSTGGGASLELLEGKELPGVVALTN 414

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BLAST of Phosphoglycerate kinase vs. TrEMBL
Match: A0A075A297 (Phosphoglycerate kinase OS=Opisthorchis viverrini OX=6198 GN=T265_00607 PE=3 SV=1)

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 312/411 (75.91%), Postives = 342/411 (83.21%), Query Frame = 1

Query:   73 KKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSD 1305
            +KLS+  +DL  KRVL+RVDFNVPMKDGK+TN QR+VAA+PTI+YALEK AKSVVLMSHLGRPDG    KYSL+PVA EVSKLLGK VKFL DCVG     AC NP  GS+FLLENLRFHVEEEGKGV  +G KIKA+ E+VK FSE LT LGDVYVNDAFGTAHRAHASMVGC LPQ+A GFL+  ELTYFAKAL+ P RPFLAIIGGAKVSDKI LIKNML+ VDELIITGGMAFTFLK L+NM+IG SL+DE G+ IV E++  AK KNVKIHLPVDF+TGDKF E A  G AT+ +G+P   MGLDCG +TM+EFC  I RAKTIVWNGPMGVFE E F  GTKAAMDAVV  T  G TTIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK LPG+ ALSD
Sbjct:    4 RKLSIANVDLKGKRVLMRVDFNVPMKDGKVTNTQRIVAALPTIQYALEKGAKSVVLMSHLGRPDGNKVDKYSLKPVAEEVSKLLGKPVKFLPDCVGPETTEACANPPAGSVFLLENLRFHVEEEGKGVSPTGEKIKATPEQVKAFSESLTKLGDVYVNDAFGTAHRAHASMVGCKLPQRACGFLMDKELTYFAKALDQPQRPFLAIIGGAKVSDKIQLIKNMLDKVDELIITGGMAFTFLKKLHNMKIGKSLYDEPGAAIVDEVMQLAKSKNVKIHLPVDFITGDKFAEDANTGTATVESGIPDTHMGLDCGPKTMEEFCKVISRAKTIVWNGPMGVFEMEKFAGGTKAAMDAVVAATTKGCTTIIGGGDTATCCAKWKTEDKVSHVSTGGGASLELLEGKQLPGILALSD 414

[Close]

BLAST of Phosphoglycerate kinase vs. TrEMBL
Match: Q45UT3 (Phosphoglycerate kinase OS=Fasciola hepatica OX=6192 PE=2 SV=2)

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 318/409 (77.75%), Postives = 349/409 (85.33%), Query Frame = 1

Query:   73 KKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAAL 1299
            KKLS+  +DL  KRVLIRVDFNVPMKDGKITNNQR+VAA+PTIKYALEK AKSVVLMSHLGRPDG    KYSL+PVA EVSKLLGK V+FL DCVGDA + AC NPA GS+FLLENLRFHVEEEGKGV  +G K+KA   +VK FSE LT LGDVYVNDAFGTAHRAHASMVGC+LPQKA GFL+  ELTYFAKALENPARPFLAIIGGAKVSDKI LIKNML+ V+ELIITGGMAFTFLK L+NM+IG SLFDE G+ IV E++  AK+K V+IHLPVDFVTG KF + A VG AT+ +G+P   MGLDCG +TM++F + I RAKTIVWNGPMGVFE E F  GTKAAMDAVV+ TK GATTIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK LPGV AL
Sbjct:    4 KKLSIANVDLKGKRVLIRVDFNVPMKDGKITNNQRIVAALPTIKYALEKGAKSVVLMSHLGRPDGNKVDKYSLKPVAEEVSKLLGKAVQFLPDCVGDATLQACSNPAPGSVFLLENLRFHVEEEGKGVGPNGEKVKADPAKVKAFSESLTKLGDVYVNDAFGTAHRAHASMVGCNLPQKACGFLMDKELTYFAKALENPARPFLAIIGGAKVSDKIQLIKNMLDKVNELIITGGMAFTFLKKLHNMKIGKSLFDEPGAAIVDEVMALAKQKKVEIHLPVDFVTGSKFGDDATVGSATVESGIPDDYMGLDCGPKTMEKFRAVILRAKTIVWNGPMGVFEMEKFAGGTKAAMDAVVEATKNGATTIIGGGDTATCCAKWKTEDKVSHVSTGGGASLELLEGKELPGVVAL 412

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BLAST of Phosphoglycerate kinase vs. Ensembl Cavefish
Match: pgk1 (phosphoglycerate kinase 1 [Source:NCBI gene;Acc:103037587])

HSP 1 Score: 560.836 bits (1444), Expect = 0.000e+0
Identity = 288/415 (69.40%), Postives = 333/415 (80.24%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 1302
            M+L  KL++DK+D+  KRV++RVDFNVPMKD  ITNNQR+ AA+P+I++ L+  AK+VVLMSHLGRPDG P   KYSLEPV AE+  LLGKDV FL DCVG  V  AC +PA GS+ LLENLRFHV EEGKG DA+GNK KAS EE+  F   L+ LGD+YVNDAFGTAHRAH+SMVG  LPQKA+GFL+K EL YFA ALE P RPFLAI+GGAKV DKI LI NML+ V+E+II GGMAFTFLKVL NMEIG SLFDE GSKIV++++ KA++  VKI LPVDFV  DKFDE+AK   AT+  G+P   MGLDCG E+ K +  A+ RAK IVWNGP+GVFE++NF  GTK  MD VV+VTK G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct:    1 MSLSNKLTLDKVDVKGKRVIMRVDFNVPMKDKAITNNQRIKAAVPSIQHCLDNGAKAVVLMSHLGRPDGVPMPDKYSLEPVVAELKTLLGKDVTFLKDCVGPEVEKACADPAAGSVILLENLRFHVAEEGKGKDAAGNKTKASQEEINSFRASLSKLGDIYVNDAFGTAHRAHSSMVGVDLPQKAAGFLMKKELDYFAMALEKPQRPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLKNMEIGTSLFDEEGSKIVKDLMAKAEKNGVKITLPVDFVIADKFDENAKTNTATVEAGIPADWMGLDCGPESSKVYSEAVSRAKQIVWNGPVGVFEWDNFAKGTKNLMDKVVEVTKNGCITIIGGGDTATCCAKWDTEDKVSHVSTGGGASLELLEGKVLPGVDALS 415

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BLAST of Phosphoglycerate kinase vs. Ensembl Cavefish
Match: pgk1 (phosphoglycerate kinase 1 [Source:NCBI gene;Acc:103037587])

HSP 1 Score: 518.85 bits (1335), Expect = 0.000e+0
Identity = 273/415 (65.78%), Postives = 318/415 (76.63%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 1302
            M+L  KL++DK+D+  KRV++R+                  AA+P+I++ L+  AK+VVLMSHLGRPDG P   KYSLEPV AE+  LLGKDV FL DCVG  V  AC +PA GS+ LLENLRFHV EEGKG DA+GNK KAS EE+  F   L+ LGD+YVNDAFGTAHRAH+SMVG  LPQKA+GFL+K EL YFA ALE P RPFLAI+GGAKV DKI LI NML+ V+E+II GGMAFTFLKVL NMEIG SLFDE GSKIV++++ KA++  VKI LPVDFV  DKFDE+AK   AT+  G+P   MGLDCG E+ K +  A+ RAK IVWNGP+GVFE++NF  GTK  MD VV+VTK G  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct:    1 MSLSNKLTLDKVDVKGKRVIMRIK-----------------AAVPSIQHCLDNGAKAVVLMSHLGRPDGVPMPDKYSLEPVVAELKTLLGKDVTFLKDCVGPEVEKACADPAAGSVILLENLRFHVAEEGKGKDAAGNKTKASQEEINSFRASLSKLGDIYVNDAFGTAHRAHSSMVGVDLPQKAAGFLMKKELDYFAMALEKPQRPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLKNMEIGTSLFDEEGSKIVKDLMAKAEKNGVKITLPVDFVIADKFDENAKTNTATVEAGIPADWMGLDCGPESSKVYSEAVSRAKQIVWNGPVGVFEWDNFAKGTKNLMDKVVEVTKNGCITIIGGGDTATCCAKWDTEDKVSHVSTGGGASLELLEGKVLPGVDALS 398

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BLAST of Phosphoglycerate kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002727.1 (pep scaffold:Pmarinus_7.0:GL491524:864:2355:-1 gene:ENSPMAG00000002492.1 transcript:ENSPMAT00000002727.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 162.54 bits (410), Expect = 5.224e-49
Identity = 81/116 (69.83%), Postives = 96/116 (82.76%), Query Frame = 1

Query:  469 NKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEI 816
            ++IKA A  V  F   L+ LGD+YVNDAFGTAHRAH+SMVGC LP KA+GFL+K EL YFAKALE P RPFLAI+GGAKV DKI LI N+L+ VDE+I+ GGMA+TFLKVL+NME+
Sbjct:    1 SQIKADAAAVAAFRASLSKLGDIYVNDAFGTAHRAHSSMVGCDLPIKAAGFLMKKELDYFAKALEKPERPFLAILGGAKVKDKIQLINNLLDKVDEMIVGGGMAYTFLKVLHNMEV 116

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BLAST of Phosphoglycerate kinase vs. Ensembl Yeast
Match: PGK1 (3-phosphoglycerate kinase; catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis [Source:SGD;Acc:S000000605])

HSP 1 Score: 495.738 bits (1275), Expect = 4.456e-175
Identity = 264/416 (63.46%), Postives = 316/416 (75.96%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEG-KGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSD 1305
            M+L  KLSV  LDL +KRV IRVDFNVP+   KIT+NQR+VAA+PTIKY LE + + VVL SHLGRP+G  N+KYSL PVA E+  LLGKDV FL+DCVG  V +A K  A GS+ LLENLR+H+EEEG + VD  G K+KAS E+V+KF   L+SL DVY+NDAFGTAHRAH+SMVG  LPQ+A+GFLL+ EL YF KALENP RPFLAI+GGAKV+DKI LI N+L+ VD +II GGMAFTF KVL N EIG+S+FD+AG++IV +++ KAK K V++ LPVDF+  D F   A     T   G+P G  GLD G E+ K F + + +AKTIVWNGP GVFEFE F  GTKA +D VV  + AG T IIGGGDTAT   K+G  DK+SHVSTGGGASLELLEGK LPGVA LS+
Sbjct:    1 MSLSSKLSVQDLDLKDKRVFIRVDFNVPLDGKKITSNQRIVAALPTIKYVLEHHPRYVVLASHLGRPNGERNEKYSLAPVAKELQSLLGKDVTFLNDCVGPEVEAAVKASAPGSVILLENLRYHIEEEGSRKVD--GQKVKASKEDVQKFRHELSSLADVYINDAFGTAHRAHSSMVGFDLPQRAAGFLLEKELKYFGKALENPTRPFLAILGGAKVADKIQLIDNLLDKVDSIIIGGGMAFTFKKVLENTEIGDSIFDKAGAEIVPKLMEKAKAKGVEVVLPVDFIIADAFSADANTKTVTDKEGIPAGWQGLDNGPESRKLFAATVAKAKTIVWNGPPGVFEFEKFAAGTKALLDEVVKSSAAGNTVIIGGGDTATVAKKYGVTDKISHVSTGGGASLELLEGKELPGVAFLSE 414

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BLAST of Phosphoglycerate kinase vs. Ensembl Yeast
Match: YCR013C_mRNA (Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant [Source:SGD;Acc:S000000606])

HSP 1 Score: 125.176 bits (313), Expect = 6.726e-34
Identity = 99/208 (47.60%), Postives = 121/208 (58.17%), Query Frame = -3

Query:  723 KILF*CLIISIFYKSDKAATPGRIFPSNNSKLAPPPVLTWLTLSSVPHLAQHXXXXXXXXXXXXXALVTSTTASIAALVPWQKFSNSNTPIGPFQTIVLAL*IALQNSFIVSLPQSNPIDPTGTPVANVA*PTLACSSNLSPVTKSTGK*ILTFFSLALLIISWTILEPASSNKLFPISMLLRTFKNVNAIPPVIISSSTMFNMFLIS 1346
            K+++     SI++ SDK ATPG   PSNNSK APPPV TW  LS  P+    VAVSPPP++ V PA    TT+S  ALVP   FSNS TP GPFQT+VLA      N+F+ S P SNP  P G P  +V    LA +   S + KSTGK   T  +LA  I   TI  PA S    PIS+  +TF  V AIPP ++  ST+ N   I+
Sbjct:    8 KLIYRFQFNSIYFFSDKKATPGNSLPSNNSKEAPPPVETWEILSVTPYFLATVAVSPPPMMTVLPAAELLTTSSNKALVPAANFSNSKTPGGPFQTMVLAFATVAANNFLDSGPLSNPCQPAGIPSLSVTVLVLASAEKASAMMKSTGKTTSTPLALAFSINFGTISAPALSKMESPISVFSKTFLKVKAIPPPMMIESTLSNKLSIN 215

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BLAST of Phosphoglycerate kinase vs. Ensembl Nematostella
Match: EDO25387 (Phosphoglycerate kinase [Source:UniProtKB/TrEMBL;Acc:A8DWM3])

HSP 1 Score: 113.235 bits (282), Expect = 6.517e-30
Identity = 63/139 (45.32%), Postives = 90/139 (64.75%), Query Frame = 1

Query:  883 KNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAAL 1299
            K V+IHLPVD +  D F   AK         +P G  GLD G  ++  F + +  +KTI+WNGP+GVFE E+F  GT A  +++   TK GA +++GGGD+     ++G E+K+S+VSTGGGA LE+LEG +LPG+ A+
Sbjct:    1 KGVQIHLPVDVIGADAFSNDAKT-QVFDVNAIPEGWQGLDAGPRSLAIFDTLLLNSKTILWNGPLGVFEMESFAKGTIALGESIAKATKNGAFSLVGGGDSVAAVKQFGLEEKMSYVSTGGGAMLEMLEGSVLPGIEAI 138

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BLAST of Phosphoglycerate kinase vs. Ensembl Medaka
Match: pgk1 (phosphoglycerate kinase 1 [Source:NCBI gene;Acc:100049274])

HSP 1 Score: 550.051 bits (1416), Expect = 0.000e+0
Identity = 289/417 (69.30%), Postives = 341/417 (81.77%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DK+D+  KRV++RVDFNVPMK  +ITNNQR+ AA+P+I++ L+  AKSVVLMSHLGRPDG    +KYSLEPVAAE+  LLG+DV FL DCVG  V +AC NPA GS+ LLENLRFHVEEEGKG DA+GNK KA+ E++  F   L+ LGDVY+NDAFGTAHRAH+SMVG +LPQKA+GFL+K EL YFA ALE P RPFLAI+GGAKV DKI LI NML+ V+E+II GGMAFTFLKVLNNMEIG SLFDE G+ IV++++ KA++  VKI LPVDF+T DKFDE A  G AT+A G+P G MGLDCG E+ K +  A+ RAK IVWNGP+GVFE+ENF  GTK  MD VV+VT+AG  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS +
Sbjct:    1 MSLSNKLTLDKVDVNGKRVVMRVDFNVPMKGRQITNNQRIKAAVPSIQHCLDHGAKSVVLMSHLGRPDGNAMPEKYSLEPVAAELKSLLGRDVAFLKDCVGPDVEAACANPAAGSVILLENLRFHVEEEGKGKDAAGNKTKATQEQIDVFRASLSKLGDVYINDAFGTAHRAHSSMVGVNLPQKAAGFLMKKELDYFAMALEKPQRPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAAIVKDLMAKAEKNGVKITLPVDFITADKFDEKAATGTATVAAGIPAGWMGLDCGPESSKAYAEAVGRAKQIVWNGPVGVFEWENFAKGTKNLMDKVVEVTQAGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVEALSSV 417

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Medaka
Match: pgk1 (phosphoglycerate kinase 1 [Source:NCBI gene;Acc:100049274])

HSP 1 Score: 535.028 bits (1377), Expect = 0.000e+0
Identity = 281/406 (69.21%), Postives = 332/406 (81.77%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGK 1275
            M+L  KL++DK+D+  KRV++RVDFNVPMK  +ITNNQR+ AA+P+I++ L+  AKSVVLMSHLGRPDG    +KYSLEPVAAE+  LLG+DV FL DCVG  V +AC NPA GS+ LLENLRFHVEEEGKG DA+GNK KA+ E++  F   L+ LGDVY+NDAFGTAHRAH+SMVG +LPQKA+GFL+K EL YFA ALE P RPFLAI+GGAKV DKI LI NML+ V+E+II GGMAFTFLKVLNNMEIG SLFDE G+ IV++++ KA++  VKI LPVDF+T DKFDE A  G AT+A G+P G MGLDCG E+ K +  A+ RAK IVWNGP+GVFE+ENF  GTK  MD VV+VT+AG  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEG+
Sbjct:    1 MSLSNKLTLDKVDVNGKRVVMRVDFNVPMKGRQITNNQRIKAAVPSIQHCLDHGAKSVVLMSHLGRPDGNAMPEKYSLEPVAAELKSLLGRDVAFLKDCVGPDVEAACANPAAGSVILLENLRFHVEEEGKGKDAAGNKTKATQEQIDVFRASLSKLGDVYINDAFGTAHRAHSSMVGVNLPQKAAGFLMKKELDYFAMALEKPQRPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAAIVKDLMAKAEKNGVKITLPVDFITADKFDEKAATGTATVAAGIPAGWMGLDCGPESSKAYAEAVGRAKQIVWNGPVGVFEWENFAKGTKNLMDKVVEVTQAGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGE 406

[Close]

BLAST of Phosphoglycerate kinase vs. Ensembl Medaka
Match: pgk1 (phosphoglycerate kinase 1 [Source:NCBI gene;Acc:100049274])

HSP 1 Score: 466.848 bits (1200), Expect = 2.509e-163
Identity = 259/417 (62.11%), Postives = 306/417 (73.38%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTP-NKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            M+L  KL++DK+D+  KRV++RVDFNVPMK  +ITNNQR+ AA+P+I++ L+  AKSVVLMSHLGRPDG    +KYSLEPVAAE+  LLG+DV FL DCVG  V +AC NPA GS+ LLENLRFHVEEEGKG DA+GNK KA+ E++  F   L+ LGDVY+NDAFGTAHRAH                                         AKV DKI LI NML+ V+E+II GGMAFTFLKVLNNMEIG SLFDE G+ IV++++ KA++  VKI LPVDF+T DKFDE A  G AT+A G+P G MGLDCG E+ K +  A+ RAK IVWNGP+GVFE+ENF  GTK  MD VV+VT+AG  TIIGGGDTATCCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS +
Sbjct:    1 MSLSNKLTLDKVDVNGKRVVMRVDFNVPMKGRQITNNQRIKAAVPSIQHCLDHGAKSVVLMSHLGRPDGNAMPEKYSLEPVAAELKSLLGRDVAFLKDCVGPDVEAACANPAAGSVILLENLRFHVEEEGKGKDAAGNKTKATQEQIDVFRASLSKLGDVYINDAFGTAHRAHR----------------------------------------AKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAAIVKDLMAKAEKNGVKITLPVDFITADKFDEKAATGTATVAAGIPAGWMGLDCGPESSKAYAEAVGRAKQIVWNGPVGVFEWENFAKGTKNLMDKVVEVTQAGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVEALSSV 377

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BLAST of Phosphoglycerate kinase vs. Planmine SMEST
Match: SMESG000039090.1 (SMESG000039090.1)

HSP 1 Score: 810.831 bits (2093), Expect = 0.000e+0
Identity = 415/416 (99.76%), Postives = 415/416 (99.76%), Query Frame = 1

Query:   61 MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 1308
            MNL KKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKAGATTIIGGGDTATCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL
Sbjct:    1 MNLSKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKAGATTIIGGGDTATCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALSDL 416

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BLAST of Phosphoglycerate kinase vs. Planmine SMEST
Match: SMESG000048946.1 (SMESG000048946.1)

HSP 1 Score: 484.567 bits (1246), Expect = 7.864e-167
Identity = 247/412 (59.95%), Postives = 319/412 (77.43%), Query Frame = 1

Query:   67 LGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYALEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVGDAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFSECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKALENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNMEIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHATLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHGTKAAMDAVVDVTKXXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 1302
            + +KL +D++++ NKRVL+RVDFNVPMK+G ITN QR+  A+PTIK AL   AKS+VL+SHLGRP G  ++KY+L PVA  +SK+L   V FL DC+G+ +  A      GS+FLLENLRF++EEEGKGV  SG K++A+A +V  F +CLT LG +YVNDAFGTAHRAHASMVG +LP + +G L+K EL YF +AL+NP RPFLAI+GGAKV+DKI+LI N+L+ ++ELIITGGMAFTFLK+L+NMEIG SL+D  G+ +VQ I+ +A   NV +HLP+DFVT ++F E A+  +AT+ +G+P G MGLD G +++  F   I R+KTI+WNGPMGVFEF+ F  G+   M+ VV  TK GA TIIGGGDTA+CCAK+ +   VSHVSTGGGASLELLEGKILPG+  L+
Sbjct:    1 MDRKLCIDEINVKNKRVLMRVDFNVPMKNGVITNMQRITEAIPTIKLALRGKAKSIVLISHLGRPQGRKDEKYTLRPVAVVLSKILDITVHFLPDCIGEYIEDAVMESPDGSVFLLENLRFYLEEEGKGVSESGQKLRANAADVASFGKCLTKLGYIYVNDAFGTAHRAHASMVGVNLPLRVAGLLMKKELKYFNRALKNPQRPFLAILGGAKVNDKIVLIFNLLDKINELIITGGMAFTFLKILHNMEIGVSLYDLDGALMVQRIMKRASVCNVTVHLPLDFVTANRFAEDARTSYATIESGIPNGWMGLDIGSKSVAAFNKVIARSKTIIWNGPMGVFEFDKFAKGSMYCMEEVVKATKNGAVTIIGGGDTASCCAKFNSAHLVSHVSTGGGASLELLEGKILPGIRVLN 412

The following BLAST results are available for this feature:

BLAST of Phosphoglycerate kinase vs. Ensembl Human
BLAST of Phosphoglycerate kinase vs. Ensembl Celegans
BLAST of Phosphoglycerate kinase vs. Ensembl Fly
BLAST of Phosphoglycerate kinase vs. Ensembl Zebrafish
BLAST of Phosphoglycerate kinase vs. Ensembl Xenopus
BLAST of Phosphoglycerate kinase vs. Ensembl Mouse
BLAST of Phosphoglycerate kinase vs. UniProt/SwissProt
BLAST of Phosphoglycerate kinase vs. TrEMBL
BLAST of Phosphoglycerate kinase vs. Ensembl Cavefish
BLAST of Phosphoglycerate kinase vs. Ensembl Sea Lamprey
BLAST of Phosphoglycerate kinase vs. Ensembl Yeast
BLAST of Phosphoglycerate kinase vs. Ensembl Nematostella
BLAST of Phosphoglycerate kinase vs. Ensembl Medaka
BLAST of Phosphoglycerate kinase vs. Planmine SMEST

BLAST of Phosphoglycerate kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3


Match Name	E-value	Identity	Description
PGK1	0.000e+0	70.74	phosphoglycerate kinase 1 [Source:HGNC Symbol;Acc:...	[more]
PGK2	0.000e+0	69.06	phosphoglycerate kinase 2 [Source:HGNC Symbol;Acc:...	[more]
PGK1	0.000e+0	71.21	phosphoglycerate kinase 1 [Source:HGNC Symbol;Acc:...	[more]

BLAST of Phosphoglycerate kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
pgk-1	0.000e+0	69.44	Probable phosphoglycerate kinase [Source:UniProtK...	[more]

BLAST of Phosphoglycerate kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Pgk	0.000e+0	67.97	gene:FBgn0250906 transcript:FBtr0077739	[more]
Pgk	0.000e+0	67.97	gene:FBgn0250906 transcript:FBtr0330718	[more]
Pgk	0.000e+0	67.97	gene:FBgn0250906 transcript:FBtr0330716	[more]
Pgk	0.000e+0	67.97	gene:FBgn0250906 transcript:FBtr0334162	[more]
Pgk	0.000e+0	67.97	gene:FBgn0250906 transcript:FBtr0330717	[more]

BLAST of Phosphoglycerate kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
pgk1	0.000e+0	69.30	phosphoglycerate kinase 1 [Source:ZFIN;Acc:ZDB-GEN...	[more]

BLAST of Phosphoglycerate kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mfsd6l	0.000e+0	68.11	major facilitator superfamily domain containing 6-...	[more]
mfsd6l	0.000e+0	68.59	major facilitator superfamily domain containing 6-...	[more]
mfsd6l	0.000e+0	64.75	major facilitator superfamily domain containing 6-...	[more]
mfsd6l	0.000e+0	64.75	major facilitator superfamily domain containing 6-...	[more]
mfsd6l	1.875e-178	63.72	major facilitator superfamily domain containing 6-...	[more]

BLAST of Phosphoglycerate kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Pgk1	0.000e+0	70.26	phosphoglycerate kinase 1 [Source:MGI Symbol;Acc:M...	[more]
Pgk2	0.000e+0	69.06	phosphoglycerate kinase 2 [Source:MGI Symbol;Acc:M...	[more]
Pgk1	9.013e-126	71.43	phosphoglycerate kinase 1 [Source:MGI Symbol;Acc:M...	[more]

BLAST of Phosphoglycerate kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P41759\|PGK_SCHMA	0.000e+0	73.17	Phosphoglycerate kinase OS=Schistosoma mansoni OX=...	[more]
sp\|Q5NVB5\|PGK1_PONAB	0.000e+0	70.74	Phosphoglycerate kinase 1 OS=Pongo abelii OX=9601 ...	[more]
sp\|Q60HD8\|PGK1_MACFA	0.000e+0	70.74	Phosphoglycerate kinase 1 OS=Macaca fascicularis O...	[more]
sp\|A5A6K4\|PGK1_PANTR	0.000e+0	70.74	Phosphoglycerate kinase 1 OS=Pan troglodytes OX=95...	[more]
sp\|P00558\|PGK1_HUMAN	0.000e+0	70.74	Phosphoglycerate kinase 1 OS=Homo sapiens OX=9606 ...	[more]

BLAST of Phosphoglycerate kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A183B9P9	0.000e+0	77.62	Phosphoglycerate kinase OS=Echinostoma caproni OX=...	[more]
A0A504Z124	0.000e+0	77.37	Phosphoglycerate kinase OS=Fasciola gigantica OX=4...	[more]
A0A4E0RCZ7	0.000e+0	77.37	Phosphoglycerate kinase OS=Fasciola hepatica OX=61...	[more]
A0A075A297	0.000e+0	75.91	Phosphoglycerate kinase OS=Opisthorchis viverrini ...	[more]
Q45UT3	0.000e+0	77.75	Phosphoglycerate kinase OS=Fasciola hepatica OX=61...	[more]

BLAST of Phosphoglycerate kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
pgk1	0.000e+0	69.40	phosphoglycerate kinase 1 [Source:NCBI gene;Acc:10...	[more]
pgk1	0.000e+0	65.78	phosphoglycerate kinase 1 [Source:NCBI gene;Acc:10...	[more]

BLAST of Phosphoglycerate kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000002727.1	5.224e-49	69.83	pep scaffold:Pmarinus_7.0:GL491524:864:2355:-1 gen...	[more]

BLAST of Phosphoglycerate kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
PGK1	4.456e-175	63.46	3-phosphoglycerate kinase; catalyzes transfer of h...	[more]
YCR013C_mRNA	6.726e-34	47.60	Dubious open reading frame; unlikely to encode a f...	[more]

BLAST of Phosphoglycerate kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO25387	6.517e-30	45.32	Phosphoglycerate kinase [Source:UniProtKB/TrEMBL;...	[more]

BLAST of Phosphoglycerate kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
pgk1	0.000e+0	69.30	phosphoglycerate kinase 1 [Source:NCBI gene;Acc:10...	[more]
pgk1	0.000e+0	69.21	phosphoglycerate kinase 1 [Source:NCBI gene;Acc:10...	[more]
pgk1	2.509e-163	62.11	phosphoglycerate kinase 1 [Source:NCBI gene;Acc:10...	[more]

BLAST of Phosphoglycerate kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000039090.1	0.000e+0	99.76	SMESG000039090.1	[more]
SMESG000048946.1	7.864e-167	59.95	SMESG000048946.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30035317 ID=SMED30035317|Name=Phosphoglycerate kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=1402bp
CTTTTGTTTAACACGAGACATATTTTGAAAATTTTATTTAGTACGTGATT
TCAACAAAAAATGAATCTAGGTAAAAAATTGTCCGTTGATAAACTTGACT
TAGCTAACAAACGAGTTCTGATTCGAGTGGATTTCAATGTGCCTATGAAA
GATGGGAAAATTACTAATAATCAAAGATTGGTTGCAGCAATGCCTACAAT
TAAATATGCATTGGAAAAAAATGCTAAATCTGTTGTTCTCATGAGCCATC
TTGGTAGACCAGATGGCACTCCAAACAAAAAATATTCTCTTGAACCAGTT
GCAGCTGAAGTGTCAAAGCTTTTGGGAAAGGATGTTAAATTCCTACATGA
TTGTGTCGGAGACGCAGTAATTTCTGCTTGTAAAAATCCTGCTCATGGAA
GTATATTTTTGCTCGAAAATCTTCGATTCCACGTCGAAGAAGAAGGAAAA
GGAGTCGATGCAAGTGGAAATAAAATTAAAGCATCTGCTGAAGAAGTTAA
AAAATTCAGTGAATGTTTGACATCATTGGGTGACGTCTACGTCAATGATG
CTTTTGGAACAGCTCACAGAGCTCACGCTTCTATGGTAGGATGTTCATTA
CCTCAAAAAGCTTCAGGATTTTTATTGAAGACTGAGTTGACTTATTTTGC
AAAGGCTTTAGAAAATCCAGCTAGACCATTTTTAGCTATTATTGGAGGAG
CTAAAGTCAGCGATAAAATCCTGCTGATTAAAAACATGTTAAACATTGTC
GACGAACTTATAATCACTGGCGGTATGGCATTTACATTTTTGAAGGTTCT
CAACAACATGGAGATTGGAAACAGTTTATTTGACGAAGCTGGCTCTAAAA
TTGTCCAGGAAATTATTAACAAAGCCAAAGAAAAAAATGTCAAAATTCAT
TTACCGGTCGATTTTGTTACTGGGGATAAATTTGATGAGCATGCCAAAGT
CGGTCATGCTACATTAGCCACCGGAGTGCCAGTAGGATCAATGGGATTGG
ATTGTGGCAAAGAGACGATGAAAGAGTTCTGCAGTGCTATTCATAGAGCT
AAAACTATCGTTTGGAATGGACCAATGGGAGTGTTTGAATTTGAAAACTT
TTGCCATGGAACCAAAGCAGCTATGGATGCCGTTGTGGACGTCACTAAAG
CGGGAGCGACAACAATTATCGGAGGAGGTGACACGGCTACATGCTGTGCT
AAATGGGGCACCGAGGACAAGGTGAGCCATGTCAGCACCGGAGGAGGAGC
CAGTTTGGAGTTGTTGGAGGGAAAGATTCTTCCCGGAGTGGCTGCTTTGT
CAGATTTGTAAAAAATCGATATTATTAGACACTAGAATAGGATTTTTCCC
TATATTTTTTGTTTTATTGAATAAATTGTGATGGCTGTTTATTAAAAAAA
AA

protein sequence of SMED30035317-orf-1

>SMED30035317-orf-1 ID=SMED30035317-orf-1|Name=SMED30035317-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=417bp
MNLGKKLSVDKLDLANKRVLIRVDFNVPMKDGKITNNQRLVAAMPTIKYA
LEKNAKSVVLMSHLGRPDGTPNKKYSLEPVAAEVSKLLGKDVKFLHDCVG
DAVISACKNPAHGSIFLLENLRFHVEEEGKGVDASGNKIKASAEEVKKFS
ECLTSLGDVYVNDAFGTAHRAHASMVGCSLPQKASGFLLKTELTYFAKAL
ENPARPFLAIIGGAKVSDKILLIKNMLNIVDELIITGGMAFTFLKVLNNM
EIGNSLFDEAGSKIVQEIINKAKEKNVKIHLPVDFVTGDKFDEHAKVGHA
TLATGVPVGSMGLDCGKETMKEFCSAIHRAKTIVWNGPMGVFEFENFCHG
TKAAMDAVVDVTKAGATTIIGGGDTATCCAKWGTEDKVSHVSTGGGASLE
LLEGKILPGVAALSDL*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000034	epidermis
PLANA:0000099	neuron
PLANA:0000101	muscle cell
PLANA:0000418	head

Vocabulary: **INTERPRO**
Term	Definition
IPR036043	Phosphoglycerate_kinase_sf
IPR007087	Znf_C2H2
IPR001576	Phosphoglycerate_kinase
IPR015824	Phosphoglycerate_kinase_N
IPR015901	Phosphoglycerate_kinase_C
IPR015911	Phosphoglycerate_kinase_CS

Vocabulary: **molecular function**
Term	Definition
GO:0046872	metal ion binding
GO:0004618	phosphoglycerate kinase activity
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0016740	transferase activity

Vocabulary: **biological process**
Term	Definition
GO:0006096	glycolytic process
GO:0016310	phosphorylation

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001576	Phosphoglycerate kinase	PRINTS	PR00477	PHGLYCKINASE	coord: 34..56 score: 48.45 coord: 208..227 score: 64.4 coord: 13..29 score: 67.81 coord: 185..207 score: 54.61 coord: 391..408 score: 86.26 coord: 332..357 score: 58.45 coord: 113..128 score: 64.56 coord: 156..178 score: 74.2 coord: 368..379 score: 61.81
IPR001576	Phosphoglycerate kinase	PFAM	PF00162	PGK	coord: 9..405 e-value: 3.6E-148 score: 493.6
IPR001576	Phosphoglycerate kinase	PIRSF	PIRSF000724	Pgk	coord: 1..416 e-value: 1.7E-136 score: 452.7
IPR001576	Phosphoglycerate kinase	PANTHER	PTHR11406	PHOSPHOGLYCERATE KINASE	coord: 3..415
IPR001576	Phosphoglycerate kinase	CDD	cd00318	Phosphoglycerate_kinase	coord: 9..415 e-value: 0.0 score: 630.441
IPR015824	Phosphoglycerate kinase, N-terminal	GENE3D	G3DSA:3.40.50.1260		coord: 75..415 e-value: 8.5E-162 score: 540.7
IPR015824	Phosphoglycerate kinase, N-terminal	GENE3D	G3DSA:3.40.50.1260		coord: 1..72 e-value: 3.6E-27 score: 97.2
IPR015824	Phosphoglycerate kinase, N-terminal	GENE3D	G3DSA:3.40.50.1260		coord: 202..391 e-value: 8.5E-162 score: 540.7
None	No IPR available	PANTHER	PTHR11406:SF0	PHOSPHOGLYCERATE KINASE	coord: 3..415
IPR015911	Phosphoglycerate kinase, conserved site	PROSITE	PS00111	PGLYCERATE_KINASE	coord: 18..28
IPR036043	Phosphoglycerate kinase superfamily	SUPERFAMILY	SSF53748	Phosphoglycerate kinase	coord: 3..415