EF-hand domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035304 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Match: sp|P14202|TEGU_SCHMA (Tegument antigen OS=Schistosoma mansoni OX=6183 GN=A12 PE=2 SV=1) HSP 1 Score: 56.6102 bits (135), Expect = 1.756e-8 Identity = 45/212 (21.23%), Postives = 82/212 (38.68%), Query Frame = 2 Query: 89 MEMLVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHL-----QKFQKLFGSHGLMNLDEFCDSLGLDINKVRQSLGYTNKNNDNNMSRQQP---VIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTP 700 ME + F +D +E I+ +EL Y + + D L +F ++++EFC GL ++++R+ K D + P +I ++SK E + E + R G +E+ ++K LD+ +W V+++ G YW + S+EP I + Y+ W+TP Sbjct: 10 MEEFIRAFLEIDADSNEMIDKQELIKYCQK-YRLDMKLIDPWIARFDT--DKDNKISIEEFCRGFGLKVSEIRREKDELKKERDGKFPKLPPNIEIIAATMSKTKQYEICCQ------------------------------FKEYVDNTSRTGNDMREVANKMKSLLDNTYGRVWQVVLLTGSYWMNFSHEPFLSIQFKYNNYVCLAWRTP 188
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8H730 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008204g1 PE=4 SV=1) HSP 1 Score: 98.5969 bits (244), Expect = 3.428e-21 Identity = 61/209 (29.19%), Postives = 103/209 (49.28%), Query Frame = 2 Query: 98 LVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHL-QKFQKLFGSH--GLMNLDEFCDSLGLD-----INKVRQSLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTP 700 +D F +D +SE I +EL Y+ ++ ++ + +K+ KLF + G + LDE+C+ LGL+ I ++ Q +NN ++P ED+ +S+ + +M+ + EI R+G EK++ R+KQQLD Q LWHV+M+ G YW+S+S+EP G++++ CWKTP Sbjct: 44 FLDAFFAVDKDRSESITPDELAAYMTSSSQFEANFGEKWMKLFDENDDGAITLDEYCNVLGLNPKQARIYRLNQLKAECLRNN----------------RLP------EDVTVIASSMGL--------------EMQVDLTEIFRDAQRRGKDEKDVAGRLKQQLDGQFGKLWHVVMVTGQYWASYSHEPGLSFFFKIGRHIVMCWKTP 216
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8JCG4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008204g2 PE=4 SV=1) HSP 1 Score: 98.5969 bits (244), Expect = 3.428e-21 Identity = 61/209 (29.19%), Postives = 103/209 (49.28%), Query Frame = 2 Query: 98 LVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHL-QKFQKLFGSH--GLMNLDEFCDSLGLD-----INKVRQSLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTP 700 +D F +D +SE I +EL Y+ ++ ++ + +K+ KLF + G + LDE+C+ LGL+ I ++ Q +NN ++P ED+ +S+ + +M+ + EI R+G EK++ R+KQQLD Q LWHV+M+ G YW+S+S+EP G++++ CWKTP Sbjct: 44 FLDAFFAVDKDRSESITPDELAAYMTSSSQFEANFGEKWMKLFDENDDGAITLDEYCNVLGLNPKQARIYRLNQLKAECLRNN----------------RLP------EDVTVIASSMGL--------------EMQVDLTEIFRDAQRRGKDEKDVAGRLKQQLDGQFGKLWHVVMVTGQYWASYSHEPGLSFFFKIGRHIVMCWKTP 216
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8H6V5 (Dynein light chain OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034129g1 PE=3 SV=1) HSP 1 Score: 96.6709 bits (239), Expect = 6.384e-21 Identity = 65/207 (31.40%), Postives = 101/207 (48.79%), Query Frame = 2 Query: 92 EMLVDTFGILDHSKSEKINLEELQNYLRNNFNYD-FHLQKFQKLFGS--HGLMNLDEFCDSLGLDINKVRQSLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPI-VTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTP 700 + +D + +D +I +L Y+R N NYD ++K+ KLF S G + LDE+C +LGL+ KV+ I+KM G SLP +TV S D+ +++ +IIEI+ R + EK++ + IKQQ D++M LWHV++I G +W +S+EP GKY+ W+TP Sbjct: 3 DAFIDAYFAIDTEGKGEITSAQLTRYMRQN-NYDEAFVRKWLKLFDSDNSGTITLDEYCQTLGLEAKKVK---------------------ADYIAKMRSG------------SLPSDITVIS----SDMTLELQLRIIEIVRDGQRLNSMEKDMAKYIKQQCDAKMERLWHVVIINGQFWCWYSHEPGYSFIFRLGKYIFCIWRTP 171
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267F2V4 (Dynein light chain (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034129g2 PE=3 SV=1) HSP 1 Score: 96.6709 bits (239), Expect = 6.982e-21 Identity = 65/207 (31.40%), Postives = 101/207 (48.79%), Query Frame = 2 Query: 92 EMLVDTFGILDHSKSEKINLEELQNYLRNNFNYD-FHLQKFQKLFGS--HGLMNLDEFCDSLGLDINKVRQSLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPI-VTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTP 700 + +D + +D +I +L Y+R N NYD ++K+ KLF S G + LDE+C +LGL+ KV+ I+KM G SLP +TV S D+ +++ +IIEI+ R + EK++ + IKQQ D++M LWHV++I G +W +S+EP GKY+ W+TP Sbjct: 7 DAFIDAYFAIDTEGKGEITSAQLTRYMRQN-NYDEAFVRKWLKLFDSDNSGTITLDEYCQTLGLEAKKVK---------------------ADYIAKMRSG------------SLPSDITVIS----SDMTLELQLRIIEIVRDGQRLNSMEKDMAKYIKQQCDAKMERLWHVVIINGQFWCWYSHEPGYSFIFRLGKYIFCIWRTP 175
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8HXX8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig028751g1 PE=4 SV=1) HSP 1 Score: 95.5153 bits (236), Expect = 3.962e-20 Identity = 58/204 (28.43%), Postives = 103/204 (50.49%), Query Frame = 2 Query: 98 LVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHL-QKFQKLFGSH--GLMNLDEFCDSLGLDINKVRQSLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTP 700 +D F +D +SE I +EL Y+ ++ ++ + +K+ KLF + G + LDE+C+ LGL+ + R + N+ + +++P ED+ +S+ + +M+ + EI R+G EK++ R+KQ+LD Q LWHV+M+ G YW+S+S+EP G++++ CWKTP Sbjct: 44 FLDAFFAVDKDRSESITPDELAAYMTSSSQFEANFGEKWMKLFDENDDGAITLDEYCNVLGLNPKQAR--IYRLNQLKAECLRS---------NRLP------EDVTVIASSMGL--------------EMQVDLTEIFRDAQRRGKDEKDVAGRLKQKLDGQFGKLWHVVMVTGQYWASYSHEPGLSFFFKIGRHIVMCWKTP 216
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000038910.1 (SMESG000038910.1) HSP 1 Score: 431.795 bits (1109), Expect = 9.287e-156 Identity = 207/209 (99.04%), Postives = 208/209 (99.52%), Query Frame = 2 Query: 89 MEMLVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHLQKFQKLFGSHGLMNLDEFCDSLGLDINKVRQSLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTPIPQDM 715 MEMLVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHLQKFQKLFGSHGLM+LDEFCDSLGLDINKVRQ LGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTPIPQDM Sbjct: 1 MEMLVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHLQKFQKLFGSHGLMSLDEFCDSLGLDINKVRQRLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTPIPQDM 209
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000038910.1 (SMESG000038910.1) HSP 1 Score: 428.328 bits (1100), Expect = 2.451e-154 Identity = 205/207 (99.03%), Postives = 206/207 (99.52%), Query Frame = 2 Query: 95 MLVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHLQKFQKLFGSHGLMNLDEFCDSLGLDINKVRQSLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTPIPQDM 715 MLVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHLQKFQKLFGSHGLM+LDEFCDSLGLDINKVRQ LGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTPIPQDM Sbjct: 1 MLVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHLQKFQKLFGSHGLMSLDEFCDSLGLDINKVRQRLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTPIPQDM 207
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026622.1 (SMESG000026622.1) HSP 1 Score: 76.2554 bits (186), Expect = 6.994e-17 Identity = 57/207 (27.54%), Postives = 98/207 (47.34%), Query Frame = 2 Query: 89 MEMLVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHLQKFQKLFGSH--GLMNLDEFCDSLGLDINKVRQSLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTPI 703 M+ + F +D +SE+I + EL+ Y+R N D ++K+ +F S+ G++ LDE+C LGL N+ R + R+Q I S +P +LP V+ +Q+ + +ME ++++I + + K ++ + IK++LD P WH + + G +WS S P GKY+ WKT I Sbjct: 1 MDNFLTAFFNIDSDQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEAR-------------IMREQFNIEHS--AIPK-------------TLP-DDVKIIQSS--VTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELDKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKTEI 176
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000008592.1 (SMESG000008592.1) HSP 1 Score: 68.5514 bits (166), Expect = 6.130e-14 Identity = 53/204 (25.98%), Postives = 95/204 (46.57%), Query Frame = 2 Query: 98 LVDTFGILDHSKSEKINLEELQNYL-RNNFNYDFHLQKFQKLF--GSHGLMNLDEFCDSLGLDINKVRQSLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRKGTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTP 700 L+ F +D S +I +EL NY+ + ++ F + K+ ++F + G + L+EFCD+LGL N+ T + M + P P E TNE+I + V ++ DPD + Q I++ + EK+I + IK++LD + +WHV++ R + S S++ ++ + W+TP Sbjct: 3 LIKAFEEIDTDYSGEITKDELINYMIKKDYEMGF-VNKWMEIFDPNNTGRITLEEFCDALGLQPNEDYLQQVQTKQEESKPMIQICP---------PFNEETNEEI---------IDVEIIREDPDTTAEFRQFAIDVSHQASKDHKLEKDIAKSIKKKLDEEYNKIWHVVVARSTFGSFISHKSGKFMHYKIENFCYLIWQTP 187
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000081370.1 (SMESG000081370.1) HSP 1 Score: 63.929 bits (154), Expect = 2.327e-12 Identity = 50/208 (24.04%), Postives = 97/208 (46.63%), Query Frame = 2 Query: 86 NMEMLVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHLQKFQKLFG--SHGLMNLDEFCDSLGLDINKVRQSLGYTNKNNDNNMSRQQPVIGPSISKMPGGEHTNEDIMKQETSLPIVTVRSLQTDPDLPKDMEQKIIEIIVGEFRK-GTAEKEIPERIKQQLDSQMAPLWHVLMIRGDYWSSHSYEPPNYITLGFGKYLITCWKTP 700 +++MLV+ F +D + + +I+ +ELQ+Y++ D ++K+ + F G ++L+EF L + +D+ R PV E + I++ + P + ++ I+ F+ G +KEI + +K++LDSQ+ LWH ++++G YW +S+EP N + F + +KTP Sbjct: 9 DVQMLVELFYEIDANCNNEISRKELQDYMKKRGYSDNDVEKWMQNFDVDHDGKISLEEFVSRLEI---------------SDDFYDR--PVT-------------------HENDVEIISA------GNTPFHRQYVMVNIVRRAFKSHGENKKEIAKFVKEKLDSQLEKLWHCVLVKGQYWGFYSHEPENSVVFKFKNTVFILFKTP 174 The following BLAST results are available for this feature:
BLAST of EF-hand domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of EF-hand domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of EF-hand domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035304 ID=SMED30035304|Name=EF-hand domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=781bpback to top protein sequence of SMED30035304-orf-1 >SMED30035304-orf-1 ID=SMED30035304-orf-1|Name=SMED30035304-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=210bp MEMLVDTFGILDHSKSEKINLEELQNYLRNNFNYDFHLQKFQKLFGSHGLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|