DNA repair protein complementing XP-A cells

Overview


Name	DNA repair protein complementing XP-A cells
Smed ID	SMED30035297
Length (bp)	1006

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of DNA repair protein complementing XP-A cells (SMED30035297) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA repair protein complementing XP-A cells (SMED30035297) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA repair protein complementing XP-A cells (SMED30035297) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30035297

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30035297	SMESG000010409.1	SmedASXL_003250	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
nervous system	SMED30035297	SMESG000010409.1	dd_Smed_v4_1950_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30035297	SMESG000010409.1	dd_Smed_v4_1950_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30035297	SMESG000010409.1	dd_Smed_v4_1950_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Human
Match: XPA (XPA, DNA damage recognition and repair factor [Source:HGNC Symbol;Acc:HGNC:12814])

HSP 1 Score: 163.696 bits (413), Expect = 6.559e-48
Identity = 102/257 (39.69%), Postives = 149/257 (57.98%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKK---RRYANLNDKEI-----ICNGKSYVDLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            EL  + RA IER RQ+AL+  + +   R Y+            +      +D   GF++++E E ++       +      F   +C  C + F +S+L  +FDL  C+ CRD + +HKLI KT AK+ YLL D DL KR+P LKFIVK+NP +S WGDMKLYL+ Q+ +RSLE+WGS+EALE  ++ R EN +KMK  K+ K +K+ R   +    K +   HQH Y    E +   D+YR+TC  CG+  +YE++
Sbjct:   19 ELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEY--GPEENLEDDMYRKTCTMCGHELTYEKM 273

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Human
Match: XPA (XPA, DNA damage recognition and repair factor [Source:HGNC Symbol;Acc:HGNC:12814])

HSP 1 Score: 145.591 bits (366), Expect = 6.178e-41
Identity = 80/192 (41.67%), Postives = 114/192 (59.38%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKK---RRYANLNDKEI-----ICNGKSYVDLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLEN 637
            EL  + RA IER RQ+AL+  + +   R Y+            +      +D   GF++++E E ++       +      F   +C  C + F +S+L  +FDL  C+ CRD + +HKLI KT AK+ YLL D DL KR+P LKFIVK+NP +S WGDMKLYL+ Q+ +RSLE+WGS+EALE  ++ R EN
Sbjct:   19 ELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQEN 210

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Celegans
Match: xpa-1 (XPA (Xeroderma pigmentosum comp grp A) related [Source:UniProtKB/TrEMBL;Acc:Q21302])

HSP 1 Score: 112.464 bits (280), Expect = 3.957e-29
Identity = 67/211 (31.75%), Postives = 116/211 (54.98%), Query Frame = 2

Query:  221 LNAGFLIDK-EIESKK--VKSFYSTKSNLSDNFTESV--CSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKE-MKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            L  GF  D  E++ ++  +      +   + N  ++   C  C +   +S+L + ++  VC+ CRD + +HKL+ +T  K  YLL D DL+ R+P L++  K+NP N  +GDMKLYL+ Q+  R LE+ GS E LE +++ R ++ +     +++K +K  R+  +    +K D+ G  H +    E    +D +RRTC  C Y + +E+L
Sbjct:   32 LGGGFCDDDDEVQERREQISEMRQKREEAAHNLIQAPENCEKCDKGLMDSWLWERYNCAVCDACRDDKGEHKLLARTEVKNTYLLKDCDLDLRKPKLRYWAKKNPHNPRYGDMKLYLKCQIETRVLEVHGSWEDLELKKELREQSKEVRSEKRFEKKLKDLRQQIRGTSGVKVDF-GRPHTHEFGKETHVEEDTWRRTCITCEYEEVFEKL 241

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Fly
Match: Xpac (gene:FBgn0004832 transcript:FBtr0070670)

HSP 1 Score: 143.665 bits (361), Expect = 3.479e-40
Identity = 97/283 (34.28%), Postives = 142/283 (50.18%), Query Frame = 2

Query:   92 LSLAERAVIERNRQKALIRLEKKRRYANLND---KEIICN---------------------GKSYVDLNAGFLIDKEIESKKVKSFYSTKSN------LSDNFTESV----CSICSETFFESFLKKNFDLIVCNKCRDP-EQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEK-EMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            L+ A++A IERN+ KA     +K R A L     KE+  N                     G  Y+D   GFL+++ +    V      KS       L D     V    C  C + F +S+L  NF   VC+KCRD  E++ LI +T AK  YLL D D +KR+P L++I ++NP N  WG+MKLYL  Q+ +R+LE+WGSEE L  + + R +  ++ K  KY K +K+ R   +     K  +  H+H +     YD  +D Y  TC  C Y ++YE++
Sbjct:   20 LTNAQKARIERNQAKA-----QKLREAKLVSHPFKELASNKEGGTHPEAALSQGSSVIKVQGTKYIDSGGGFLLEQPVMPTGVGPAGLNKSGEEAPPILDDAIAIPVQYEECLECGDMFADSYLFNNFGHSVCDKCRDKDERYALITRTEAKAEYLLKDCDFDKREPKLRYISRKNPHNVRWGEMKLYLHLQIHQRALEVWGSEEELVRQHEAREDKREEGKARKYNKKMKQLRMEVRSSIYTKKTHEVHEHEFGPDT-YDEEEDTYTHTCITCPYSETYEKM 296

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Zebrafish
Match: xpa (xeroderma pigmentosum, complementation group A [Source:ZFIN;Acc:ZDB-GENE-040426-1205])

HSP 1 Score: 155.992 bits (393), Expect = 1.199e-42
Identity = 92/239 (38.49%), Postives = 139/239 (58.16%), Query Frame = 2

Query:  110 AVIERNRQKALIRLEKKRRYANLNDKEIICNGKSY------VDLNAGFLIDKEIESKKV---KSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTC 796
            A IERNRQ+AL+   ++ R A+     +   G ++      +D  AGF I++E    +    +        +  ++   +C  C + F +S+L  +FDL VC+KCRD E +HKLI++T AK+ YLL D DL+KR+P L+FI+++NP N  WGDMKLYL+ QV +RS+E+WGS+EALE  ++TR EN +  K  ++ K +K+ R   +    K D   HQH Y      D  +D +   C
Sbjct:   33 AKIERNRQRALML--RQARLASRPSSAV--EGATHAKVAKTIDSGAGFFIEEETTEDEQQEKRVVQQPAPVMEPDYL--MCEECQKPFMDSYLSNSFDLSVCDKCRDNEVKHKLISRTEAKKNYLLKDCDLDKREPPLRFILRKNPHNPRWGDMKLYLKTQVEKRSVEVWGSDEALEEAKETREENKEVQKQKRFNKKVKELRRTVRSSMFKKDTSIHQHDYGPDELLDEEEDSFSEKC 265

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Xenopus
Match: xpa (xeroderma pigmentosum, complementation group A [Source:NCBI gene;Acc:549490])

HSP 1 Score: 174.866 bits (442), Expect = 2.665e-52
Identity = 110/260 (42.31%), Postives = 158/260 (60.77%), Query Frame = 2

Query:   74 CKMSEE-LSLAERAVIERNRQKALIRLEKK---RRYANLNDKEIICNGKSYVDLNAGFLIDKE-IESKKVKSF-YSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPEQ-HKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            CK  E+ L  A RA IERNRQ+AL+  + +   R Y        +      +D   GF I++E  E + V++  +     L  ++   +C  C + F +S+L  +FDL VC+ CRD E+ HKLI +T AK+ YLL D D+++R+P LKFI+K+NP NS+WGDMKLYL+ QV +RSLE+WGSEEALE  ++ R +N DKMK  K+ K +K+ R   +    K +  GHQH Y    E    +D Y++TC  CGY  +YE++
Sbjct:   10 CKEEEKILPAAVRAKIERNRQRALMLRQARLACRPYPTGEGISTVKALPKVIDSGGGFFIEEEDAEEQHVENVVHQPGPVLECDYL--ICEECGKDFMDSYLSNHFDLAVCDSCRDAEEKHKLITRTEAKQEYLLKDCDIDRREPVLKFILKKNPHNSNWGDMKLYLKTQVIKRSLEVWGSEEALEEAKEVRKDNRDKMKQKKFDKKVKELRRAVRSSLWKKEASGHQHDY--GPEEHVEEDSYKKTCITCGYEMNYEKM 265

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Xenopus
Match: xpa (xeroderma pigmentosum, complementation group A [Source:NCBI gene;Acc:549490])

HSP 1 Score: 152.14 bits (383), Expect = 6.736e-44
Identity = 87/197 (44.16%), Postives = 124/197 (62.94%), Query Frame = 2

Query:   74 CKMSEE-LSLAERAVIERNRQKALIRLEKK---RRYANLNDKEIICNGKSYVDLNAGFLIDKE-IESKKVKSF-YSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPEQ-HKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENID 643
            CK  E+ L  A RA IERNRQ+AL+  + +   R Y        +      +D   GF I++E  E + V++  +     L  ++   +C  C + F +S+L  +FDL VC+ CRD E+ HKLI +T AK+ YLL D D+++R+P LKFI+K+NP NS+WGDMKLYL+ QV +RSLE+WGSEEALE  ++ R +N D
Sbjct:   10 CKEEEKILPAAVRAKIERNRQRALMLRQARLACRPYPTGEGISTVKALPKVIDSGGGFFIEEEDAEEQHVENVVHQPGPVLECDYL--ICEECGKDFMDSYLSNHFDLAVCDSCRDAEEKHKLITRTEAKQEYLLKDCDIDRREPVLKFILKKNPHNSNWGDMKLYLKTQVIKRSLEVWGSEEALEEAKEVRKDNRD 204

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Mouse
Match: Xpa (xeroderma pigmentosum, complementation group A [Source:MGI Symbol;Acc:MGI:99135])

HSP 1 Score: 166.777 bits (421), Expect = 3.599e-49
Identity = 102/256 (39.84%), Postives = 153/256 (59.77%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGKSYV-------DLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            +L  A RA +ER RQ+AL+ L + R  A          G + V       D   GF++++E E  ++ +       + + F  ++C  C + F +S+L  +FDL  C+ CRD + +HKLI KT AK+ YLL D DL KR+P L+F+VK+NP++S WGDMKLYL+ QV +R+LE+WGS+EALE  ++ R EN +KMK  K+ K +K+ R   +    K +   HQH Y    E +   D+YR+TC  CG+  +YE++
Sbjct:   21 QLPAAVRASVERKRQRALM-LRQARLAARPYPAAAATGGVASVKAAPKMIDTKGGFILEEEEEKHEIGNIVHEPGPVME-FDYTICEECGKEFMDSYLMNHFDLPTCDSCRDADDKHKLITKTEAKQEYLLKDCDLEKREPALRFLVKKNPRHSQWGDMKLYLKLQVVKRALEVWGSQEALEDAKEVRQENREKMKQKKFDKKVKELRRAIRSSVWKRETTTHQHKY--GPEENLEDDMYRKTCTLCGHELTYEKM 272

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Mouse
Match: Xpa (xeroderma pigmentosum, complementation group A [Source:MGI Symbol;Acc:MGI:99135])

HSP 1 Score: 147.517 bits (371), Expect = 7.782e-42
Identity = 80/191 (41.88%), Postives = 118/191 (61.78%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGKSYV-------DLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLEN 637
            +L  A RA +ER RQ+AL+ L + R  A          G + V       D   GF++++E E  ++ +       + + F  ++C  C + F +S+L  +FDL  C+ CRD + +HKLI KT AK+ YLL D DL KR+P L+F+VK+NP++S WGDMKLYL+ QV +R+LE+WGS+EALE  ++ R EN
Sbjct:   21 QLPAAVRASVERKRQRALM-LRQARLAARPYPAAAATGGVASVKAAPKMIDTKGGFILEEEEEKHEIGNIVHEPGPVME-FDYTICEECGKEFMDSYLMNHFDLPTCDSCRDADDKHKLITKTEAKQEYLLKDCDLEKREPALRFLVKKNPRHSQWGDMKLYLKLQVVKRALEVWGSQEALEDAKEVRQEN 209

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Mouse
Match: Xpa (xeroderma pigmentosum, complementation group A [Source:MGI Symbol;Acc:MGI:99135])

HSP 1 Score: 147.132 bits (370), Expect = 1.329e-41
Identity = 80/191 (41.88%), Postives = 118/191 (61.78%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGKSYV-------DLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLEN 637
            +L  A RA +ER RQ+AL+ L + R  A          G + V       D   GF++++E E  ++ +       + + F  ++C  C + F +S+L  +FDL  C+ CRD + +HKLI KT AK+ YLL D DL KR+P L+F+VK+NP++S WGDMKLYL+ QV +R+LE+WGS+EALE  ++ R EN
Sbjct:   21 QLPAAVRASVERKRQRALM-LRQARLAARPYPAAAATGGVASVKAAPKMIDTKGGFILEEEEEKHEIGNIVHEPGPVME-FDYTICEECGKEFMDSYLMNHFDLPTCDSCRDADDKHKLITKTEAKQEYLLKDCDLEKREPALRFLVKKNPRHSQWGDMKLYLKLQVVKRALEVWGSQEALEDAKEVRQEN 209

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Mouse
Match: Xpa (xeroderma pigmentosum, complementation group A [Source:MGI Symbol;Acc:MGI:99135])

HSP 1 Score: 125.946 bits (315), Expect = 3.538e-35
Identity = 57/99 (57.58%), Postives = 77/99 (77.78%), Query Frame = 2

Query:  344 FFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLEN 637
            F +S+L  +FDL  C+ CRD + +HKLI KT AK+ YLL D DL KR+P L+F+VK+NP++S WGDMKLYL+ QV +R+LE+WGS+EALE  ++ R EN
Sbjct:    2 FMDSYLMNHFDLPTCDSCRDADDKHKLITKTEAKQEYLLKDCDLEKREPALRFLVKKNPRHSQWGDMKLYLKLQVVKRALEVWGSQEALEDAKEVRQEN 100

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BLAST of DNA repair protein complementing XP-A cells vs. UniProt/SwissProt
Match: sp|P27088|XPA_XENLA (DNA repair protein complementing XP-A cells homolog OS=Xenopus laevis OX=8355 GN=xpa PE=2 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 2.199e-51
Identity = 106/251 (42.23%), Postives = 149/251 (59.36%), Query Frame = 2

Query:   92 LSLAERAVIERNRQKALIRLEKK---RRYANLNDKEIICNGKSYVDLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPEQ-HKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            LS A RA IERNRQ+AL+  + +   R Y        +      +D   GF I++E   ++       +          +C  C + F +S+L  +FDL VC+ CRD E+ HKLI +T AK+ YLL D D++KR+P LKFI+K+NP N+ WGDMKLYL+AQV +RSLE+WGSEEALE  ++ R +N DKMK  K+ K +K+ R   +    K +  GHQH Y    E    +D Y++TC  CGY  +YE++
Sbjct:   19 LSAAVRAKIERNRQRALMLRQARLACRPYPTGEGISTVKAPPKVIDSGGGFFIEEEEAEEQHVENVVRQPGPVLECDYLICEECGKDFMDSYLSNHFDLAVCDSCRDAEEKHKLITRTEAKQEYLLKDCDIDKREPVLKFILKKNPHNTHWGDMKLYLKAQVIKRSLEVWGSEEALEEAKEVRKDNRDKMKQKKFDKKVKELRRTVRSSLWKKEASGHQHEY--GPEEHVEEDSYKKTCITCGYEMNYEKM 267

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BLAST of DNA repair protein complementing XP-A cells vs. UniProt/SwissProt
Match: sp|Q64267|XPA_MOUSE (DNA repair protein complementing XP-A cells homolog OS=Mus musculus OX=10090 GN=Xpa PE=1 SV=2)

HSP 1 Score: 166.777 bits (421), Expect = 2.517e-48
Identity = 102/256 (39.84%), Postives = 153/256 (59.77%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGKSYV-------DLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            +L  A RA +ER RQ+AL+ L + R  A          G + V       D   GF++++E E  ++ +       + + F  ++C  C + F +S+L  +FDL  C+ CRD + +HKLI KT AK+ YLL D DL KR+P L+F+VK+NP++S WGDMKLYL+ QV +R+LE+WGS+EALE  ++ R EN +KMK  K+ K +K+ R   +    K +   HQH Y    E +   D+YR+TC  CG+  +YE++
Sbjct:   21 QLPAAVRASVERKRQRALM-LRQARLAARPYPAAAATGGVASVKAAPKMIDTKGGFILEEEEEKHEIGNIVHEPGPVME-FDYTICEECGKEFMDSYLMNHFDLPTCDSCRDADDKHKLITKTEAKQEYLLKDCDLEKREPALRFLVKKNPRHSQWGDMKLYLKLQVVKRALEVWGSQEALEDAKEVRQENREKMKQKKFDKKVKELRRAIRSSVWKRETTTHQHKY--GPEENLEDDMYRKTCTLCGHELTYEKM 272

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BLAST of DNA repair protein complementing XP-A cells vs. UniProt/SwissProt
Match: sp|P23025|XPA_HUMAN (DNA repair protein complementing XP-A cells OS=Homo sapiens OX=9606 GN=XPA PE=1 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 3.150e-47
Identity = 102/257 (39.69%), Postives = 149/257 (57.98%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKK---RRYANLNDKEI-----ICNGKSYVDLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            EL  + RA IER RQ+AL+  + +   R Y+            +      +D   GF++++E E ++       +      F   +C  C + F +S+L  +FDL  C+ CRD + +HKLI KT AK+ YLL D DL KR+P LKFIVK+NP +S WGDMKLYL+ Q+ +RSLE+WGS+EALE  ++ R EN +KMK  K+ K +K+ R   +    K +   HQH Y    E +   D+YR+TC  CG+  +YE++
Sbjct:   19 ELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEY--GPEENLEDDMYRKTCTMCGHELTYEKM 273

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BLAST of DNA repair protein complementing XP-A cells vs. UniProt/SwissProt
Match: sp|P27089|XPA_CHICK (DNA repair protein complementing XP-A cells homolog OS=Gallus gallus OX=9031 GN=XPA PE=2 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 1.041e-42
Identity = 75/177 (42.37%), Postives = 115/177 (64.97%), Query Frame = 2

Query:  305 NFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
             F   +C  C + F +S+L ++FD   C+ CRD E +HKLI +T AKE YLL D DL+KR+P L+FIVK+NP N  WGDMKLYL+ QV +R+LE+WG+EE+L+  ++ R ++ +KMK  ++ K +K+ R   +    K     H+H Y    E +  ++ Y++TC  CG+  +YE++
Sbjct:   93 QFDYLICGDCGKEFMDSYLMQHFDWATCDNCRDAEDKHKLITRTEAKEEYLLKDCDLDKREPVLRFIVKKNPHNPRWGDMKLYLKLQVIKRALEVWGNEESLQEAKEQRRDSREKMKQKRFDKKVKELRRAVRSSLWKKTASIHEHEY--GPEENVDEETYKKTCTVCGHELTYEKM 267

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BLAST of DNA repair protein complementing XP-A cells vs. UniProt/SwissProt
Match: sp|P28518|XPA_DROME (DNA repair protein complementing XP-A cells homolog OS=Drosophila melanogaster OX=7227 GN=Xpac PE=2 SV=3)

HSP 1 Score: 143.665 bits (361), Expect = 3.487e-39
Identity = 97/283 (34.28%), Postives = 142/283 (50.18%), Query Frame = 2

Query:   92 LSLAERAVIERNRQKALIRLEKKRRYANLND---KEIICN---------------------GKSYVDLNAGFLIDKEIESKKVKSFYSTKSN------LSDNFTESV----CSICSETFFESFLKKNFDLIVCNKCRDP-EQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEK-EMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            L+ A++A IERN+ KA     +K R A L     KE+  N                     G  Y+D   GFL+++ +    V      KS       L D     V    C  C + F +S+L  NF   VC+KCRD  E++ LI +T AK  YLL D D +KR+P L++I ++NP N  WG+MKLYL  Q+ +R+LE+WGSEE L  + + R +  ++ K  KY K +K+ R   +     K  +  H+H +     YD  +D Y  TC  C Y ++YE++
Sbjct:   20 LTNAQKARIERNQAKA-----QKLREAKLVSHPFKELASNKEGGTHPEAALSQGSSVIKVQGTKYIDSGGGFLLEQPVMPTGVGPAGLNKSGEEAPPILDDAIAIPVQYEECLECGDMFADSYLFNNFGHSVCDKCRDKDERYALITRTEAKAEYLLKDCDFDKREPKLRYISRKNPHNVRWGEMKLYLHLQIHQRALEVWGSEEELVRQHEAREDKREEGKARKYNKKMKQLRMEVRSSIYTKKTHEVHEHEFGPDT-YDEEEDTYTHTCITCPYSETYEKM 296

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BLAST of DNA repair protein complementing XP-A cells vs. TrEMBL
Match: A0A2I4CG41 (DNA repair protein complementing XP-A cells OS=Austrofundulus limnaeus OX=52670 GN=xpa PE=4 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 1.218e-57
Identity = 110/255 (43.14%), Postives = 155/255 (60.78%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGK--SYVDLNAGFLIDKE----IESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRD-PEQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            ELS A RA IERNRQ+AL+  + +     L+  E   + K    +D  AGF I++E     E K  K  +     +   +   +C  C + F +SFL  +FDL VC+KCRD  E+HKLI++T AK+ YLL D DL+KR+P L+FI+++NP NS WGDMKLYL+ QV +R LE+WGSEEALE  R+TR EN +  K  ++ K +K+ R   +      +   HQH Y  +   D  +DLY++TC  CG+  SYE++
Sbjct:   18 ELSAAMRAKIERNRQRALMLRQARLASRPLSAVEGATSAKVSKTIDSGAGFFIEEEEYGGTEQKANKVVHQPAPVIEPEYL--LCDDCQKPFMDSFLSNSFDLPVCDKCRDNEEKHKLISRTEAKQRYLLKDCDLDKREPPLRFILRKNPHNSCWGDMKLYLQLQVVKRCLEVWGSEEALEEARETREENKEVQKQKRFNKKVKELRRAVRSSMWTKNTSVHQHEYGPEEVVDPEEDLYKKTCTTCGHELSYEKM 270

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BLAST of DNA repair protein complementing XP-A cells vs. TrEMBL
Match: A0A147A513 (DNA repair protein complementing XP-A cells OS=Fundulus heteroclitus OX=8078 PE=4 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 1.559e-56
Identity = 110/255 (43.14%), Postives = 156/255 (61.18%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGK--SYVDLNAGFLIDKEIES----KKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRD-PEQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            ELS A RA IERNRQ+AL+  + +     L+  E   + K    VD  AGF ID+E +     K  K  +     +  ++   +C  C + F +SFL  +FDL VC+KCRD  E+HKLI++T AK+ YLL D+DL+KR+P L+FI+K+NP NS WGDMKLYL  QV +R +E+WGSEEALE  R+TR EN +  K  ++ K +K+ R   +      +   HQH Y  +   D  +DLY++TC  CG+  +YE++
Sbjct:   23 ELSAAMRAKIERNRQRALMLRQARLASRPLSAVEGATSAKVSKTVDSGAGFFIDEEEDGEDERKATKVVHQPAPVIEPDYL--LCDDCQKPFMDSFLSNSFDLSVCDKCRDNEEKHKLISRTEAKQRYLLKDVDLDKREPPLRFILKKNPHNSHWGDMKLYLLLQVEKRCMEVWGSEEALEEARETREENKEVQKQKRFNKKVKELRRAVRSSMWTKNTSAHQHEYGPEEVVDPEEDLYKKTCTTCGHELTYEKM 275

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BLAST of DNA repair protein complementing XP-A cells vs. TrEMBL
Match: A0A3Q2YG03 (Xeroderma pigmentosum, complementation group A OS=Hippocampus comes OX=109280 PE=4 SV=1)

HSP 1 Score: 193.356 bits (490), Expect = 3.848e-56
Identity = 107/258 (41.47%), Postives = 158/258 (61.24%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKK---RRYANLNDKEI--ICNGKSYVDLNAGFLIDKE--IESKKV--KSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDP-EQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            ELS A RA IERNRQ+AL+  + +   R +A      +  +C     +D  AGF I++E  ++ K+   +  +     +  ++    C  C + F +S+L  +FDL VC+KCRD  E+HKLI++T AK+IYLL D DL+KR+P LKFI+K+NP N  WGDMKLYL+  V +RS+E+WGSE ALE  ++TR EN +  K  ++ K +K+ R   +      D   HQH Y  +V  D  +DLY++TC  CG+  +YE++
Sbjct:   27 ELSAAMRAKIERNRQRALMLRQARIASRPFAAEQSATLAKVCKT---IDTGAGFFIEEEEGLQEKQTANRVVHQPAPVIEPDYL--FCDDCQKPFMDSYLSNSFDLSVCDKCRDSTEKHKLISRTEAKQIYLLKDCDLDKREPPLKFILKKNPHNPRWGDMKLYLKIHVEKRSMEVWGSEAALEEAKETREENREVQKQKRFNKKVKELRRAVRSSMWTKDTSAHQHEYGPEVVVDPEEDLYKKTCRTCGHELTYEKM 279

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BLAST of DNA repair protein complementing XP-A cells vs. TrEMBL
Match: A0A3B5MJH1 (Xeroderma pigmentosum, complementation group A OS=Xiphophorus couchianus OX=32473 PE=4 SV=1)

HSP 1 Score: 193.356 bits (490), Expect = 4.065e-56
Identity = 107/255 (41.96%), Postives = 155/255 (60.78%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGK--SYVDLNAGFLIDKEIES----KKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRD-PEQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            ELS A RA IERNRQ+AL+  + +     L+  E   + K    +D  AGF I++E       K  K  +     +  ++   +C  C + F +SFL  +FDL VC+KCRD  E+HKLI++T AK+ YLL D+DL+KR+P L+FI+K+NP N  WGDMKLYL  QV +R +E+WGSEEALE  R+TR EN +  K  ++ K +++ R   +      + R HQH Y  +   D  +DLY++TC  CG+  +YE++
Sbjct:   23 ELSAAMRAKIERNRQRALMLRQARLASRPLSAVEGATSAKVSKTIDSGAGFFIEEEEGGEEERKAKKVVHQPAPVIEPDYL--LCDDCQKPFMDSFLSNSFDLSVCDKCRDNEEKHKLISRTEAKQRYLLKDVDLDKREPPLRFILKKNPHNPHWGDMKLYLLLQVEKRCMEVWGSEEALEEARETREENKEVQKQKRFNKKVRELRRAVRSSTWTKNTRAHQHEYGPEEVVDPEEDLYKKTCTTCGHELTYEKM 275

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BLAST of DNA repair protein complementing XP-A cells vs. TrEMBL
Match: A0A3Q1HRW1 (Xeroderma pigmentosum, complementation group A OS=Anabas testudineus OX=64144 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.004e-55
Identity = 108/255 (42.35%), Postives = 155/255 (60.78%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGK--SYVDLNAGFLIDKEIES----KKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRD-PEQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            ELS A RA IERNRQ+AL+  + +     L+  E   + K    +D  AGF I++E +     K  K  +     +  ++   VC  C + F +S+L  +FDL VC+KCRD  E+HKLI++T AK+ YLL D DL+KR+P L+FI+K+NP N  WGDMKLYL+ QV +R +E+WGSEEALE  R+TR EN +  K  ++ K +K+ R   +      D   HQH Y  +   D  +DLY++TC  CG+  +YE++
Sbjct:   23 ELSAAMRAKIERNRQRALMLRQARLASRPLSAAEGATSAKVSKTIDSGAGFFIEEEEDGEEDQKARKVVHQPAPVIEPDYL--VCDDCEKPFMDSYLSNSFDLSVCDKCRDNEEKHKLISRTEAKQSYLLKDCDLDKREPPLRFILKKNPHNPRWGDMKLYLKLQVEKRCMEVWGSEEALEEARETREENKEVQKQKRFNKKVKELRRAVRSSMWTKDTSVHQHQYGPEEVVDPEEDLYKKTCSTCGHELTYEKM 275

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Cavefish
Match: xpa (xeroderma pigmentosum, complementation group A [Source:ZFIN;Acc:ZDB-GENE-040426-1205])

HSP 1 Score: 172.555 bits (436), Expect = 1.382e-51
Identity = 101/256 (39.45%), Postives = 152/256 (59.38%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGKSYV----DLNAGFLIDKEIES---KKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
             L  A  A IERNRQ+AL+ L+++   A     E + +  + V    D  AGF I+ E +    + +K  +     +  ++   +C  CS  F +S+L  +FDL VC+KCRD + +H+LI++T AK  YLL D DL+ R+P L+F++K+NP N  WGDMKLYL+ QV +RSL++WGSEEALE  R++R EN +  K  ++ K +K+ R   +    K D   HQH Y  +   D  +D YR+ C  CG+  +YE++
Sbjct:   21 PLPPAVLARIERNRQRALL-LDRRASPAQRPLLETVRSTAAKVSKTIDSGAGFFIEDEEDDEEQRTIKVVHKPAPVMEPDYL--MCEECSRPFMDSYLSNSFDLSVCDKCRDNDVKHRLISRTEAKHQYLLKDCDLDLREPPLRFVLKKNPHNPRWGDMKLYLKTQVVKRSLDVWGSEEALEEARESREENREVQKQKRFNKKVKELRRAVRSSMFKKDTSVHQHDYGPEEVVDEEEDQYRKVCKTCGHQLTYEKM 273

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Cavefish
Match: ENSAMXT00000033108.1 (pep primary_assembly:Astyanax_mexicanus-2.0:21:5200073:5204441:-1 gene:ENSAMXG00000037903.1 transcript:ENSAMXT00000033108.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 98.2117 bits (243), Expect = 4.028e-24
Identity = 55/140 (39.29%), Postives = 82/140 (58.57%), Query Frame = 2

Query:  413 HKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            H+LI++T AK  YLL D DL+ R+P L+F++K+NP N             V +RSL++WGSEEALE  R++R EN +  K  ++ K +K+ R   +    K D   HQH Y  +   D  +D YR+ C  CG+  +YE++
Sbjct:   71 HRLISRTEAKHQYLLKDCDLDLREPPLRFVLKKNPHNP------------VVKRSLDVWGSEEALEEARESREENREVQKQKRFNKKVKELRRAVRSSMFKKDTSVHQHDYGPEEVVDEEEDQYRKVCKTCGHQLTYEKM 198

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Cavefish
Match: ENSAMXT00000051486.1 (pep primary_assembly:Astyanax_mexicanus-2.0:21:5200073:5204441:-1 gene:ENSAMXG00000037903.1 transcript:ENSAMXT00000051486.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 74.7146 bits (182), Expect = 1.468e-15
Identity = 49/140 (35.00%), Postives = 74/140 (52.86%), Query Frame = 2

Query:  413 HKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            H+LI++T AK  YLL D DL+ R+P L+F++K+NP N       +Y              S EALE  R++R EN +  K  ++ K +K+ R   +    K D   HQH Y  +   D  +D YR+ C  CG+  +YE++
Sbjct:   71 HRLISRTEAKHQYLLKDCDLDLREPPLRFVLKKNPHNPVNIYCTIY------------CFSIEALEEARESREENREVQKQKRFNKKVKELRRAVRSSMFKKDTSVHQHDYGPEEVVDEEEDQYRKVCKTCGHQLTYEKM 198

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Sea Lamprey
Match: XPA (XPA, DNA damage recognition and repair factor [Source:HGNC Symbol;Acc:HGNC:12814])

HSP 1 Score: 153.68 bits (387), Expect = 1.468e-45
Identity = 76/210 (36.19%), Postives = 122/210 (58.10%), Query Frame = 2

Query:  215 VDLNAGFLIDKEIESKKV---KSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            VD   GFL+++  + ++    +  ++    +  ++ +  C  C   F +S+L   FDL VC+ CRD E +H+L+++T AK  +LL D DL+ R+P L+F++++NP N  WGDMKLYL  QV  RSL++WG EEAL+  R+ R    D+ +  ++   +K+ R   +    +    GH H Y  +   DA  D Y RTC QCG+  +YE++
Sbjct:   16 VDTGGGFLLEEADDEEEAAARRVVHTPAPVMKADYLQ--CGECGRRFMDSYLSATFDLAVCDACRDNEGKHQLVSRTEAKTEFLLKDCDLDGREPALRFVLRKNPHNPRWGDMKLYLRLQVEGRSLDVWGGEEALQEAREQRHHGRDRRQQKRFNNKVKELRMAVRSSLYRKASAGHVHEYGPEQRDDADADAYHRTCVQCGHRLAYEKM 223

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Yeast
Match: RAD14 (Protein that recognizes and binds damaged DNA during NER; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein; NER stands for nucleotide excision repair [Source:SGD;Acc:S000004814])

HSP 1 Score: 61.6178 bits (148), Expect = 3.636e-11
Identity = 54/174 (31.03%), Postives = 82/174 (47.13%), Query Frame = 2

Query:  347 FESFLKKNFDLIVCNKC--RDPEQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEM------KFDYRGHQHVYLDKVEYDAAQDLY---RRTCGQCGYVDSYEELD 835
             +  L   F L VC +C    PE++ L+ KT  KE Y L D +LN        + K NP + ++  M+L++  +V   + + WG EE L+ E + R E     +  KY+K IK+ R   + +E       K   + H H + D V+    +D Y   RR C  CG     EE+D
Sbjct:  200 MDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED-LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCTDCGL--ETEEID 370

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Nematostella
Match: EDO34508 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SP61])

HSP 1 Score: 47.7506 bits (112), Expect = 1.293e-6
Identity = 28/69 (40.58%), Postives = 41/69 (59.42%), Query Frame = 2

Query:  422 INKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTR 628
            I K +AK  YLL+D DL +    L    K NP N S+  MK++  ++V  +++E+WGS E LE E+  R
Sbjct:   13 IAKGNAKRDYLLSDEDLKE----LNHETKDNPINKSYPPMKIFKLSEVKSKAVEVWGSLEKLEDEKTKR 77

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BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Medaka
Match: xpa (XPA, DNA damage recognition and repair factor [Source:NCBI gene;Acc:101167183])

HSP 1 Score: 167.162 bits (422), Expect = 2.468e-49
Identity = 110/260 (42.31%), Postives = 150/260 (57.69%), Query Frame = 2

Query:   89 ELSLAERAVIERNRQKALIRLEKKRRYANLNDKE--IICNGKS-----YVDLNAGFLIDKEIESKKVKSFYS-TKSNLSDNFTES---VCSICSETFFESFLKKNFDLIVCNKCRDPE-QHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIKKQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEEL 832
            ELS A RA IERNRQ+AL+      R A L  +    +  G S      +D  AGF ID+E E        S T         E    +C  C + F +S L  +FDL VC+KCRD E +HKLI++T AK+ YLL D DL+KR+P L+FI+K+NP N  WGDMKLYL+ Q  +R+LE+WGSEEA+E  R+ R EN +  K  ++ K +K+ R   +      +  GHQH Y  +   D   DLYR+TC  CG+  ++E++
Sbjct:   23 ELSAAMRAKIERNRQRALML-----RQARLASRPSAAVEGGTSAKVSRTIDSGAGFFIDEEEEGGGGGEGKSRTVVQEPAPVIEPEYLLCDDCQKPFMDSVLSNSFDLSVCDKCRDTEGKHKLISRTEAKQRYLLKDCDLDKREPPLRFILKKNPHNPRWGDMKLYLQLQAEKRALEVWGSEEAVEEAREAREENREVQKQKRFNKKVKELRRAVRSSVWTKNTSGHQHEYGPEEVLDPEDDLYRKTCTSCGHEVTFEKM 277

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BLAST of DNA repair protein complementing XP-A cells vs. Planmine SMEST
Match: SMESG000010409.1 (SMESG000010409.1)

HSP 1 Score: 325.865 bits (834), Expect = 2.485e-113
Identity = 160/161 (99.38%), Postives = 160/161 (99.38%), Query Frame = 2

Query:   80 MSEELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGKSYVDLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPEQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSSWGDMKLYLEAQ 562
            MSEELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGKSYVDLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPEQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVK NPKNSSWGDMKLYLEAQ
Sbjct:    1 MSEELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGKSYVDLNAGFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDLIVCNKCRDPEQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKPNPKNSSWGDMKLYLEAQ 161

The following BLAST results are available for this feature:

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Human
BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Celegans
BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Fly
BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Zebrafish
BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Xenopus
BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Mouse
BLAST of DNA repair protein complementing XP-A cells vs. UniProt/SwissProt
BLAST of DNA repair protein complementing XP-A cells vs. TrEMBL
BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Cavefish
BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Sea Lamprey
BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Yeast
BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Nematostella
BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Medaka
BLAST of DNA repair protein complementing XP-A cells vs. Planmine SMEST

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
XPA	6.559e-48	39.69	XPA, DNA damage recognition and repair factor [Sou...	[more]
XPA	6.178e-41	41.67	XPA, DNA damage recognition and repair factor [Sou...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
xpa-1	3.957e-29	31.75	XPA (Xeroderma pigmentosum comp grp A) related [S...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Xpac	3.479e-40	34.28	gene:FBgn0004832 transcript:FBtr0070670	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
xpa	1.199e-42	38.49	xeroderma pigmentosum, complementation group A [So...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
xpa	2.665e-52	42.31	xeroderma pigmentosum, complementation group A [So...	[more]
xpa	6.736e-44	44.16	xeroderma pigmentosum, complementation group A [So...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4


Match Name	E-value	Identity	Description
Xpa	3.599e-49	39.84	xeroderma pigmentosum, complementation group A [So...	[more]
Xpa	7.782e-42	41.88	xeroderma pigmentosum, complementation group A [So...	[more]
Xpa	1.329e-41	41.88	xeroderma pigmentosum, complementation group A [So...	[more]
Xpa	3.538e-35	57.58	xeroderma pigmentosum, complementation group A [So...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P27088\|XPA_XENLA	2.199e-51	42.23	DNA repair protein complementing XP-A cells homolo...	[more]
sp\|Q64267\|XPA_MOUSE	2.517e-48	39.84	DNA repair protein complementing XP-A cells homolo...	[more]
sp\|P23025\|XPA_HUMAN	3.150e-47	39.69	DNA repair protein complementing XP-A cells OS=Hom...	[more]
sp\|P27089\|XPA_CHICK	1.041e-42	42.37	DNA repair protein complementing XP-A cells homolo...	[more]
sp\|P28518\|XPA_DROME	3.487e-39	34.28	DNA repair protein complementing XP-A cells homolo...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A2I4CG41	1.218e-57	43.14	DNA repair protein complementing XP-A cells OS=Aus...	[more]
A0A147A513	1.559e-56	43.14	DNA repair protein complementing XP-A cells OS=Fun...	[more]
A0A3Q2YG03	3.848e-56	41.47	Xeroderma pigmentosum, complementation group A OS=...	[more]
A0A3B5MJH1	4.065e-56	41.96	Xeroderma pigmentosum, complementation group A OS=...	[more]
A0A3Q1HRW1	1.004e-55	42.35	Xeroderma pigmentosum, complementation group A OS=...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
xpa	1.382e-51	39.45	xeroderma pigmentosum, complementation group A [So...	[more]
ENSAMXT00000033108.1	4.028e-24	39.29	pep primary_assembly:Astyanax_mexicanus-2.0:21:520...	[more]
ENSAMXT00000051486.1	1.468e-15	35.00	pep primary_assembly:Astyanax_mexicanus-2.0:21:520...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
XPA	1.468e-45	36.19	XPA, DNA damage recognition and repair factor [Sou...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
RAD14	3.636e-11	31.03	Protein that recognizes and binds damaged DNA duri...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO34508	1.293e-6	40.58	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
xpa	2.468e-49	42.31	XPA, DNA damage recognition and repair factor [Sou...	[more]

BLAST of DNA repair protein complementing XP-A cells vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000010409.1	2.485e-113	99.38	SMESG000010409.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30035297 ID=SMED30035297|Name=DNA repair protein complementing XP-A cells|organism=Schmidtea mediterranea sexual|type=transcript|length=1006bp
GTACCTAGTATTTTCAAATTTTGATTTTACAAGTCTATTGTTTTAAAATG
GTGATTATTTGAATAATTATTTTTGTAAAATGAGTGAAGAATTAAGCTTA
GCGGAAAGAGCTGTTATTGAACGAAATCGACAAAAAGCTCTTATAAGACT
TGAGAAAAAGCGTCGATATGCCAATTTGAATGACAAAGAAATTATTTGTA
ATGGTAAAAGTTATGTTGACTTAAACGCTGGATTTCTCATTGATAAAGAA
ATTGAAAGTAAAAAAGTGAAAAGCTTTTATTCTACTAAATCAAACTTGTC
GGATAATTTTACTGAATCTGTTTGTAGTATTTGTTCAGAAACATTTTTCG
AATCATTTTTAAAAAAAAATTTCGACTTGATCGTTTGTAATAAATGTCGG
GACCCGGAGCAGCATAAGCTTATTAACAAAACCTCTGCCAAAGAAATCTA
TCTGCTTAACGACATTGATTTGAATAAACGCCAGCCGAATCTAAAATTCA
TAGTAAAACAGAATCCCAAGAACTCGTCTTGGGGCGACATGAAACTCTAT
CTGGAAGCCCAAGTCGCAGAGCGATCTCTAGAGATCTGGGGATCCGAGGA
AGCCCTGGAAACCGAGCGGAAGACTCGATTGGAAAATATTGACAAAATGA
AAATTATGAAATATCAGAAAAGCATCAAAAAGCAGCGGGAAAACGCCAAG
GAAAAGGAGATGAAATTCGATTATCGGGGACATCAGCACGTTTATCTGGA
CAAGGTAGAGTACGACGCAGCTCAGGATTTGTACCGACGCACGTGCGGGC
AATGTGGCTATGTCGATAGCTATGAGGAGCTCGATTAATAAATGGCGCGT
TATCTTTTGATATATTATCATTATTGATTAATTTTATTATATTATTGATT
AATTTTTTTATGTCATTGATTAATTTTATAATTTTTACTAGGACAATTTA
TTAATGCTCAATAAATGCAGAATTATAACTCTGCAATTTTATGCATCTAT
TTGCAC

protein sequence of SMED30035297-orf-1

>SMED30035297-orf-1 ID=SMED30035297-orf-1|Name=SMED30035297-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=253bp
MSEELSLAERAVIERNRQKALIRLEKKRRYANLNDKEIICNGKSYVDLNA
GFLIDKEIESKKVKSFYSTKSNLSDNFTESVCSICSETFFESFLKKNFDL
IVCNKCRDPEQHKLINKTSAKEIYLLNDIDLNKRQPNLKFIVKQNPKNSS
WGDMKLYLEAQVAERSLEIWGSEEALETERKTRLENIDKMKIMKYQKSIK
KQRENAKEKEMKFDYRGHQHVYLDKVEYDAAQDLYRRTCGQCGYVDSYEE
LD*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **cellular component**
Term	Definition
GO:0042025	host cell nucleus
GO:0005634	nucleus

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000429	neoblast
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR022656	XPA_C
IPR022652	Znf_XPA_CS
IPR009061	DNA-bd_dom_put_sf

Vocabulary: **molecular function**
Term	Definition
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0046872	metal ion binding

Vocabulary: **biological process**
Term	Definition
GO:0006281	DNA repair
GO:0006289	nucleotide-excision repair
GO:0006974	cellular response to DNA damage stimulus

Main menu

DNA repair protein complementing XP-A cells

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

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