SMED30035268
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035268 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30035268 aligns in the following genomic locations:
Homology
BLAST of SMED30035268 vs. Ensembl Fly
Match: CG13392 (gene:FBgn0032033 transcript:FBtr0079720) HSP 1 Score: 51.9878 bits (123), Expect = 1.340e-7 Identity = 42/152 (27.63%), Postives = 73/152 (48.03%), Query Frame = 3 Query: 303 RPNEMVPQNGKIINILDISLIRRLRMI-NWVPQEDVWVLRKGSYLLCAFTVLSGSLTILNIRRFLQLQNQRLPTTLFGGLIVPVLLNGTAGEKYITSTIL-EGLYNCPLCMELRGSVLQAMFGSVVPLVLSFFICIPAAKLEKTYPVPKLTE 752 +P+E+ P++ +I D +L + ++I +W +VW LR +L A +G+ + R L+L +T + VP + A + +I IL L CP+C+++R + Q G V P +L+ F A TY +P +TE Sbjct: 7 KPDEL-PRDAVVIT-EDQALKYQWKIITSWDKIGEVWSLRYTPGILSALAAGTGAYINNHYRTKLRLGGHGRLSTYLPIVAVPAIFTMLAHKFFIQRPILLNPLGECPVCIQMRSAAFQTSLGIVYPTILAPFAAFLFATRCYTYRIPSITE 156
BLAST of SMED30035268 vs. Ensembl Fly
Match: CG13392 (gene:FBgn0032033 transcript:FBtr0331995) HSP 1 Score: 51.9878 bits (123), Expect = 1.340e-7 Identity = 42/152 (27.63%), Postives = 73/152 (48.03%), Query Frame = 3 Query: 303 RPNEMVPQNGKIINILDISLIRRLRMI-NWVPQEDVWVLRKGSYLLCAFTVLSGSLTILNIRRFLQLQNQRLPTTLFGGLIVPVLLNGTAGEKYITSTIL-EGLYNCPLCMELRGSVLQAMFGSVVPLVLSFFICIPAAKLEKTYPVPKLTE 752 +P+E+ P++ +I D +L + ++I +W +VW LR +L A +G+ + R L+L +T + VP + A + +I IL L CP+C+++R + Q G V P +L+ F A TY +P +TE Sbjct: 7 KPDEL-PRDAVVIT-EDQALKYQWKIITSWDKIGEVWSLRYTPGILSALAAGTGAYINNHYRTKLRLGGHGRLSTYLPIVAVPAIFTMLAHKFFIQRPILLNPLGECPVCIQMRSAAFQTSLGIVYPTILAPFAAFLFATRCYTYRIPSITE 156
BLAST of SMED30035268 vs. TrEMBL
Match: A0A068YDN9 (tRNA specific adenosine deaminase protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000801800 PE=4 SV=1) HSP 1 Score: 84.3445 bits (207), Expect = 1.220e-15 Identity = 55/209 (26.32%), Postives = 101/209 (48.33%), Query Frame = 3 Query: 309 NEMVPQNGKIINILDISLIRRLRMINWVPQEDVWVLRKGSYLLCAFTVLSGSLTILNIRRFLQLQNQRLPTTLFGGLIVPVLLNGTAG-----EKYITSTILEGLYN-------CPLCMELRGSVLQAMFGSVVPLVLSFFICIPAAKLEKTYPVPKLTEFYKYFDIIKLALNRGKYTFLTLGVLNFFYGGLISNRMMECNKRMDARDE 899 NE+V ++ K+++ +W PQ VW L G+ + S + ++ +R+F L +Q+ L G L+ PV++ +AG + ++ ++ G C C+E+RGS +Q + G V P++ S C A+ +T+PVP+ T++ K F ++ A T + L + N GG I+ +M+E + + A E Sbjct: 5 NEVVKKHRKVVS-------------SWHPQTSVWALAHGATMSALCVAGSSYMVLIRMRQFFNLYSQQY---LLGTLL-PVVILPSAGYIILQDAFVDRKLISGFSLPETRKSLCSTCLEIRGSFIQMVSGVVAPVLFSVSSCASASISYRTFPVPEFTKYRKVFGLVCKACGSFSSTLILLTLANAVVGGFITEKMVEQHALVSATRE 196
BLAST of SMED30035268 vs. TrEMBL
Match: A0A068WB22 (tRNA specific adenosine deaminase protein OS=Echinococcus granulosus OX=6210 GN=EgrG_000801800 PE=4 SV=1) HSP 1 Score: 82.8037 bits (203), Expect = 5.446e-15 Identity = 55/211 (26.07%), Postives = 101/211 (47.87%), Query Frame = 3 Query: 309 NEMVPQNGKIINILDISLIRRLRMINWVPQEDVWVLRKGSYLLCAFTVLSGSLTILNIRRFLQLQNQRLPTTLFGGLIVPVLLNGTAG-----EKYITSTILEGLYN-------CPLCMELRGSVLQAMFGSVVPLVLSFFICIPAAKLEKTYPVPKLTEFYKYFDIIKLALNRGKYTFLTLGVLNFFYGGLISNRMMECNKRMDARDEYV 905 NE+V ++ K+++ +W PQ VW L G+ + S + ++ +R+F L +Q+ L G L+ PV+ + G + ++ ++ G C C+E+RGS +Q + G V P++ S C A+ +T+PVP+LT++ K F ++ A T + L + N GG I+ +M+E + + A E + Sbjct: 5 NEVVKEHRKVVS-------------SWHPQTSVWALAHGATMSALCVAGSSYMVLIRMRQFFNLYSQQY---LLGTLL-PVVALPSVGYIILQDVFVDRKLISGFSLPETRKSLCSTCLEIRGSFIQMVAGVVAPVLFSVSSCASASISYRTFPVPELTKYRKVFGLVCKACGSFSSTLILLTLANAVVGGFITEKMVEQHALVSATRETI 198
BLAST of SMED30035268 vs. TrEMBL
Match: A0A433SMM1 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_021822 PE=4 SV=1) HSP 1 Score: 82.4185 bits (202), Expect = 1.018e-14 Identity = 62/206 (30.10%), Postives = 97/206 (47.09%), Query Frame = 3 Query: 255 MEERSINPMFGVIVNRRPNEMVPQNGKIINILDISLIRRLRMINWVPQEDVWVLRKGSYLLCAFTVLSGSLTILNI-RRFLQLQNQRLPTTLFGGLIVPVLLNGTAGEKYITSTILEGLYNCPLCMELRGSVLQAMFGSVVPLVLSFFICIPAAKLEKTYPVPKLTEFYKYFDIIKLALNRGKYTFLTLGVLNFFYGGLISNRMME 869 M+++ + M G V + E +PQN + ++ I+ R+ + PQE V V SY + A T +SG L I N+ RR +L R T +++ L G E I IL G +CP C+ ++G LQA G V P VLS CIP A+ T P Y +++ + + F L +LN + G ++ R ++ Sbjct: 1 MDQQPVK-MRGEAVLFKRGERIPQNALPLQDAEVLSIQTSRINKFQPQEKVRVYHYSSYAVSATTSISG-LVIANVMRRNFRLGGLRKVLTYVPTVVISGLCAGAMQEILIKRQILLGKESCPPCVAVKGGCLQAGLGVVYPYVLSLLTCIPTARDYYTLQFPGRGSNSSYLQLLRQVSPKSSWMF-GLALLNIYVGSYVAVRQLQ 203
BLAST of SMED30035268 vs. TrEMBL
Match: A0A0R3UQW6 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS10272 PE=4 SV=1) HSP 1 Score: 81.6481 bits (200), Expect = 1.448e-14 Identity = 56/177 (31.64%), Postives = 87/177 (49.15%), Query Frame = 3 Query: 366 RRLRMINWVPQEDVWVLRKGSYLLCAFTVLSGSLTIL-NIRRFLQL-QNQRLPTTLFGGLIVPVLLNGTAGEKYITSTILE-------GLYNCPLCMELRGSVLQAMFGSVVPLVLSFFICIPAAKLEKTYPVPKLTEFYKYFDIIKLALNRGKYTFLTLGVLNFFYGGLISNRMME 869 +R + W PQ DVW L G+ + A V S +IL +R+F L Q L T+ +++P + A + +I + G + C C+E+RGS +QA G P +LS F C A+ +T+PVP L +F K +++ A T + L + N F GG I+ RM + Sbjct: 10 QRKTVSAWNPQTDVWALAHGTKI-SALCVAGSSFSILMRMRQFFSLFSQQHLLGTILPVVVLPSMGYIIANDIFIERPFVTRLSRPNIGQHFCSTCLEVRGSFIQATAGVFAPFLLSVFSCAAASISYRTFPVPDLGDFRKVLSLVRRAGGSLSSTLILLTLANAFVGGFITLRMAD 185
BLAST of SMED30035268 vs. TrEMBL
Match: A0A0X3Q661 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR92340 PE=4 SV=1) HSP 1 Score: 75.485 bits (184), Expect = 2.208e-12 Identity = 56/183 (30.60%), Postives = 87/183 (47.54%), Query Frame = 3 Query: 366 RRLRMI-NWVPQEDVWVLRKGSYLLCAFTVLSGSLTILNIRRFLQLQNQRLPTTLFGGLIVPVLLNGTAGEKYITSTILEGLYN------------CPLCMELRGSVLQAMFGSVVPLVLSFFICIPAAKLEKTYPVPKLTEFYKYFDIIKLALNRGKYTFLTLGVLNFFYGGLISNRMMECN 875 R+L+ + +W PQ +VW L G + + S IL +R+F L +Q+ F G I+PV+ T G + +++ + C C ELRGSVLQA G V P +LS F C AA +TYP P L ++ K I+ + T L + N +GG ++ M++ + Sbjct: 14 RQLQQLRHWQPQSEVWPLVHGPKMASVCVMASSFAIILRMRQFYNLFSQQH----FFGTIIPVVSLPTIGYLIASDLLVKRCLSSQFANYDSTRPICTTCYELRGSVLQAAMGVVFPYMLSMFACSAAAISYRTYPTPPLNDYRKMLLTIRRSCRALGTTLTLLTLANLAFGGWLTRSMLDTH 192
BLAST of SMED30035268 vs. Ensembl Medaka
Match: tmem126a (transmembrane protein 126A [Source:ZFIN;Acc:ZDB-GENE-040426-1770]) HSP 1 Score: 54.6842 bits (130), Expect = 1.643e-8 Identity = 42/161 (26.09%), Postives = 74/161 (45.96%), Query Frame = 3 Query: 390 VPQEDVWVLRKGSYLLCAFTVLSGSLTILNIRRFLQLQNQRLPTTLFGGLIVPVLLNGTAGEKYITSTILEGLYNCPLCMELRGSVLQAMFGSVVPLVLSFFICIPAAKLEKTYPVPKLTEFYKY-FDIIKLALNRGKYTFLTLGVLNFFYGGLISNRMME 869 +P D + G + L L+G ++ RR L + RL ++L ++P L ++ +L G NCP C +RG+++ + G+V P+VL+ + I A T P+P +Y D+ + LN+ + L L F+G + +R E Sbjct: 24 LPDLDQKLFTLGPFYLAGNAGLAGLISNSLYRRALNIHAARLASSLPMA-VLPFLTTSALYSATVSQPLLSGDLNCPTCAMIRGALVGVVGGAVYPIVLALPLNIGLASRYSTAPLPGKGNLLRYCVDVSRPVLNKMRAVVL----LQAFFGTYLGSRNFE 179
BLAST of SMED30035268 vs. Planmine SMEST
Match: SMESG000008500.1 (SMESG000008500.1) HSP 1 Score: 457.603 bits (1176), Expect = 1.954e-164 Identity = 224/225 (99.56%), Postives = 225/225 (100.00%), Query Frame = 3 Query: 243 MKGNMEERSINPMFGVIVNRRPNEMVPQNGKIINILDISLIRRLRMINWVPQEDVWVLRKGSYLLCAFTVLSGSLTILNIRRFLQLQNQRLPTTLFGGLIVPVLLNGTAGEKYITSTILEGLYNCPLCMELRGSVLQAMFGSVVPLVLSFFICIPAAKLEKTYPVPKLTEFYKYFDIIKLALNRGKYTFLTLGVLNFFYGGLISNRMMECNKRMDARDEYVYQKL 917 MKGNMEERSINPMFGVIVNRRPNEMVPQNGKIINILDISLIRRLRMINWVPQEDVWVLRKGSYLLCAFTVLSGSLTILNIRRFLQLQNQRLPTTLFGGLIVPVLLNGTAGEKYITSTILEGLYNCPLCMELRGSVLQAMFGS+VPLVLSFFICIPAAKLEKTYPVPKLTEFYKYFDIIKLALNRGKYTFLTLGVLNFFYGGLISNRMMECNKRMDARDEYVYQKL Sbjct: 1 MKGNMEERSINPMFGVIVNRRPNEMVPQNGKIINILDISLIRRLRMINWVPQEDVWVLRKGSYLLCAFTVLSGSLTILNIRRFLQLQNQRLPTTLFGGLIVPVLLNGTAGEKYITSTILEGLYNCPLCMELRGSVLQAMFGSIVPLVLSFFICIPAAKLEKTYPVPKLTEFYKYFDIIKLALNRGKYTFLTLGVLNFFYGGLISNRMMECNKRMDARDEYVYQKL 225 The following BLAST results are available for this feature:
BLAST of SMED30035268 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30035268 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30035268 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of SMED30035268 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30035268 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30035268 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30035268 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30035268 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30035268 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30035268 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30035268 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30035268 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30035268 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of SMED30035268 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035268 ID=SMED30035268|Name=SMED30035268|organism=Schmidtea mediterranea sexual|type=transcript|length=968bpback to top protein sequence of SMED30035268-orf-1 >SMED30035268-orf-1 ID=SMED30035268-orf-1|Name=SMED30035268-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=240bp MKGNMEERSINPMFGVIVNRRPNEMVPQNGKIINILDISLIRRLRMINWVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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