Sprouty related EVH1 domain containing 2a

Overview
NameSprouty related EVH1 domain containing 2a
Smed IDSMED30035248
Length (bp)606
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Sprouty related EVH1 domain containing 2a (SMED30035248) t-SNE clustered cells

Violin plots show distribution of expression levels for Sprouty related EVH1 domain containing 2a (SMED30035248) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Sprouty related EVH1 domain containing 2a (SMED30035248) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Sprouty related EVH1 domain containing 2a (SMED30035248) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035248

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30035248h1SMcG0002496 Contig35398uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30035248h1SMcG0002496 Contig35398newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30035248h1SMcG0002496 dd_Smed_v4_14816_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30035248h1SMcG0002496 dd_Smed_v4_14816_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30035248h1SMcG0002496 dd_Smed_v4_14816_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30035248h1SMcG0002496 dd_Smed_v4_14816_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Human
Match: SPRED1 (sprouty related EVH1 domain containing 1 [Source:HGNC Symbol;Acc:HGNC:20249])

HSP 1 Score: 65.4698 bits (158), Expect = 4.893e-12
Identity = 34/92 (36.96%), Postives = 49/92 (53.26%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP----WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPL 401
            C YC + +    NV+G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP      + +    W   +    LS ++PC+CCY PL
Sbjct:  335 CVYCQERFNHEENVRGKCQDAPDPIKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRW---LALVALSFIVPCMCCYVPL 423
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Human
Match: SPRED2 (sprouty related EVH1 domain containing 2 [Source:HGNC Symbol;Acc:HGNC:17722])

HSP 1 Score: 64.3142 bits (155), Expect = 1.001e-11
Identity = 40/107 (37.38%), Postives = 49/107 (45.79%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC  M+    N +G C +APD     I  VSCM CA    Y    D +GDY DP         +  R +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  306 CVYCRDMFNHEENRRGHCQDAPDSVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACYHCGVMCRCCGGKHK 412
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Human
Match: SPRED2 (sprouty related EVH1 domain containing 2 [Source:HGNC Symbol;Acc:HGNC:17722])

HSP 1 Score: 64.3142 bits (155), Expect = 1.014e-11
Identity = 40/110 (36.36%), Postives = 50/110 (45.45%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP----WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC  M+    N +G C +APD     I  VSCM CA    Y    D +GDY DP      + +    W   +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  324 CVYCRDMFNHEENRRGHCQDAPDSVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRW---MALIALSFLAPCMCCYLPLRACYHCGVMCRCCGGKHK 430
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Human
Match: SPRED2 (sprouty related EVH1 domain containing 2 [Source:HGNC Symbol;Acc:HGNC:17722])

HSP 1 Score: 64.3142 bits (155), Expect = 1.160e-11
Identity = 40/110 (36.36%), Postives = 50/110 (45.45%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP----WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC  M+    N +G C +APD     I  VSCM CA    Y    D +GDY DP      + +    W   +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  309 CVYCRDMFNHEENRRGHCQDAPDSVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRW---MALIALSFLAPCMCCYLPLRACYHCGVMCRCCGGKHK 415
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Human
Match: SPRED3 (sprouty related EVH1 domain containing 3 [Source:HGNC Symbol;Acc:HGNC:31041])

HSP 1 Score: 50.447 bits (119), Expect = 5.401e-7
Identity = 40/115 (34.78%), Postives = 59/115 (51.30%), Query Frame = 3 
Query:  126 DSYVCTYCFQMYADSRNVKGS-CSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPN-WRDRIKWYLLSLVLPCLCCYGPLVRLQH--SAR-KLSLCGGKHE 455
            ++  C +C  ++    + +G  C+EAPDP   ++  +SC+ CA+   Y    D +GD+ DP       P           LSL +PCLCCY PL R  H  +AR   + CGG+HE
Sbjct:  293 EAARCVHCRALFRRRADGRGGRCAEAPDPGRLLVRRLSCLWCAESLLYHCLSDAEGDFSDPCACEPGHPRPAARWAALAALSLAVPCLCCYAPL-RACHWVAARCGCAGCGGRHE 406
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Fly
Match: Spred (gene:FBgn0020767 transcript:FBtr0087468)

HSP 1 Score: 57.3806 bits (137), Expect = 1.447e-9
Identity = 35/109 (32.11%), Postives = 54/109 (49.54%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDY-EDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHEP 458
            C YC ++Y++  N +G+C  APD      + +S M CA+   Y    D +G+  + P   +        R +   +LSL +PCL CY PL     +     LCGG+H+P
Sbjct:  316 CRYCHELYSEDFNRRGACEYAPDAFRSGYECISGMGCARCMIYHCMSDAEGETAQHPCDCSASEAGCSKRWLGLAILSLFVPCLWCYPPLRACHLAGIHCGLCGGQHKP 424
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Fly
Match: Spred (gene:FBgn0020767 transcript:FBtr0329879)

HSP 1 Score: 56.9954 bits (136), Expect = 1.454e-9
Identity = 35/109 (32.11%), Postives = 54/109 (49.54%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDY-EDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHEP 458
            C YC ++Y++  N +G+C  APD      + +S M CA+   Y    D +G+  + P   +        R +   +LSL +PCL CY PL     +     LCGG+H+P
Sbjct:  294 CRYCHELYSEDFNRRGACEYAPDAFRSGYECISGMGCARCMIYHCMSDAEGETAQHPCDCSASEAGCSKRWLGLAILSLFVPCLWCYPPLRACHLAGIHCGLCGGQHKP 402
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Fly
Match: Spred (gene:FBgn0020767 transcript:FBtr0329880)

HSP 1 Score: 56.6102 bits (135), Expect = 2.266e-9
Identity = 37/119 (31.09%), Postives = 54/119 (45.38%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSP------------NWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHEP 458
            C YC ++Y++  N +G+C  APD      + +S M CA+   Y    D +G+       T Q P             W   +   +LSL +PCL CY PL     +     LCGG+H+P
Sbjct:  316 CRYCHELYSEDFNRRGACEYAPDAFRSGYECISGMGCARCMIYHCMSDAEGE-------TAQHPCDCSASEAGCSKRW---LGLAILSLFVPCLWCYPPLRACHLAGIHCGLCGGQHKP 424
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Zebrafish
Match: spred1 (sprouty related EVH1 domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2338])

HSP 1 Score: 68.9366 bits (167), Expect = 7.762e-14
Identity = 48/142 (33.80%), Postives = 64/142 (45.07%), Query Frame = 3 
Query:   39 FLNELQIRFFIEKKLMSVNKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKW---YLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            F  ELQ      K      K    +R   +   C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP         +   ++W     LSL+ PCLCCY PL            CGGKH+
Sbjct:  112 FSEELQKDSV--KTQQPSGKASRRRREDGERSRCIYCREMFNHEDNQRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDASEEQF--CVRWLALLGLSLLAPCLCCYPPLRLCHRCGEACRCCGGKHK 249
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Zebrafish
Match: spred1 (sprouty related EVH1 domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2338])

HSP 1 Score: 67.3958 bits (163), Expect = 5.187e-13
Identity = 48/142 (33.80%), Postives = 64/142 (45.07%), Query Frame = 3 
Query:   39 FLNELQIRFFIEKKLMSVNKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKW---YLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            F  ELQ      K      K    +R   +   C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP         +   ++W     LSL+ PCLCCY PL            CGGKH+
Sbjct:  251 FSEELQKDSV--KTQQPSGKASRRRREDGERSRCIYCREMFNHEDNQRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDASEEQF--CVRWLALLGLSLLAPCLCCYPPLRLCHRCGEACRCCGGKHK 388
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Zebrafish
Match: spred1 (sprouty related EVH1 domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2338])

HSP 1 Score: 67.3958 bits (163), Expect = 5.521e-13
Identity = 48/142 (33.80%), Postives = 64/142 (45.07%), Query Frame = 3 
Query:   39 FLNELQIRFFIEKKLMSVNKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKW---YLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            F  ELQ      K      K    +R   +   C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP         +   ++W     LSL+ PCLCCY PL            CGGKH+
Sbjct:  245 FSEELQKDSV--KTQQPSGKASRRRREDGERSRCIYCREMFNHEDNQRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDASEEQF--CVRWLALLGLSLLAPCLCCYPPLRLCHRCGEACRCCGGKHK 382
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Zebrafish
Match: spred2a (sprouty related EVH1 domain containing 2a [Source:ZFIN;Acc:ZDB-GENE-110830-2])

HSP 1 Score: 65.855 bits (159), Expect = 1.827e-12
Identity = 48/122 (39.34%), Postives = 62/122 (50.82%), Query Frame = 3 
Query:  105 LMKRV--SADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKW---YLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            L +RV  S +   C YC  M++ S N +G C EAPDP +  I  VS M CA    Y    D +GDY DP   +  +   R  ++W     LSL+ PC+CCY PL    H       CGGKH+
Sbjct:  299 LPRRVEDSRERLRCVYCQNMFSPSENGRGRCQEAPDPVQTCIRRVSFMWCADSLLYHCMSDPEGDYSDPC--SCDASEERFCLRWLALLGLSLLAPCMCCYVPLRACYHCGVACHCCGGKHK 418
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Zebrafish
Match: spred2a (sprouty related EVH1 domain containing 2a [Source:ZFIN;Acc:ZDB-GENE-110830-2])

HSP 1 Score: 65.4698 bits (158), Expect = 2.407e-12
Identity = 44/109 (40.37%), Postives = 56/109 (51.38%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKW---YLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC  M++ S N +G C EAPDP +  I  VS M CA    Y    D +GDY DP   +  +   R  ++W     LSL+ PC+CCY PL    H       CGGKH+
Sbjct:  305 CVYCQNMFSPSENGRGRCQEAPDPVQTCIRRVSFMWCADSLLYHCMSDPEGDYSDPC--SCDASEERFCLRWLALLGLSLLAPCMCCYVPLRACYHCGVACHCCGGKHK 411
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Xenopus
Match: spred1 (sprouty related, EVH1 domain containing 1 [Source:NCBI gene;Acc:493399])

HSP 1 Score: 66.2402 bits (160), Expect = 1.965e-12
Identity = 41/107 (38.32%), Postives = 53/107 (49.53%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC + +    N +G C +APDP ++ I  VSCM CA+   Y    D +GDY DP        N   R +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  297 CVYCQERFNHEENGRGKCQDAPDPIQRCIYQVSCMLCAESMLYHCMSDSEGDYSDPCSCDASDENLCLRWLALITLSFIAPCMCCYLPLRACHHCGEMCGCCGGKHK 403
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Xenopus
Match: gpat3 (glycerol-3-phosphate acyltransferase 3 [Source:Xenbase;Acc:XB-GENE-993435])

HSP 1 Score: 63.5438 bits (153), Expect = 1.595e-11
Identity = 34/91 (37.36%), Postives = 50/91 (54.95%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKW---YLLSLVLPCLCCYGPL 401
            C YC  ++    N +G C +APDP  + +  +SCM CA+   Y    D +G+Y DP       P++   ++W     LSLV+PC+CCY PL
Sbjct:  315 CVYCRDVFTSEENGRGQCQDAPDPIGRCVYQLSCMWCAESLLYHCMSDSEGEYSDPCSCDPGHPHF--CVRWAALVALSLVVPCMCCYLPL 403
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Xenopus
Match: spred2 (sprouty related, EVH1 domain containing 2 [Source:NCBI gene;Acc:496441])

HSP 1 Score: 52.373 bits (124), Expect = 1.272e-7
Identity = 33/100 (33.00%), Postives = 48/100 (48.00%), Query Frame = 3 
Query:  111 KRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKWYL---LSLVLPCLCCYGPL 401
            +R   +   C YC  M+    N +G C +APD     I  VSCM  A    Y    D +GDY D    +  + + +  ++W L   LS++ P +CCY PL
Sbjct:  295 RRKDGERSRCIYCRDMFNHDENRRGQCQDAPDSVRNCIRRVSCMGFADCVLYHCMSDSEGDYTDAC--SCDTSDEKFCLRWMLLIPLSIIAPGMCCYLPL 392
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Xenopus
Match: spred2 (sprouty related, EVH1 domain containing 2 [Source:NCBI gene;Acc:496441])

HSP 1 Score: 51.9878 bits (123), Expect = 1.632e-7
Identity = 33/100 (33.00%), Postives = 48/100 (48.00%), Query Frame = 3 
Query:  111 KRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKWYL---LSLVLPCLCCYGPL 401
            +R   +   C YC  M+    N +G C +APD     I  VSCM  A    Y    D +GDY D    +  + + +  ++W L   LS++ P +CCY PL
Sbjct:  291 RRKDGERSRCIYCRDMFNHDENRRGQCQDAPDSVRNCIRRVSCMGFADCVLYHCMSDSEGDYTDAC--SCDTSDEKFCLRWMLLIPLSIIAPGMCCYLPL 388
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Mouse
Match: Spred1 (sprouty protein with EVH-1 domain 1, related sequence [Source:MGI Symbol;Acc:MGI:2150016])

HSP 1 Score: 64.3142 bits (155), Expect = 8.774e-12
Identity = 33/92 (35.87%), Postives = 48/92 (52.17%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP----WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPL 401
            C YC + +    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP      + +    W   +    LS ++PC+CCY PL
Sbjct:  335 CVYCQERFNHEENARGKCQDAPDPVKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRW---LALVALSFIVPCMCCYVPL 423
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Mouse
Match: Spred2 (sprouty-related EVH1 domain containing 2 [Source:MGI Symbol;Acc:MGI:2150019])

HSP 1 Score: 62.7734 bits (151), Expect = 2.179e-11
Identity = 39/116 (33.62%), Postives = 52/116 (44.83%), Query Frame = 3 
Query:  111 KRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            ++ + +   C YC  M+    N +G C +APD     I  VSCM CA    Y    D +GDY DP         +  R +    LS + PC+CCY PL            CGGKH+
Sbjct:  292 RKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHK 407
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Mouse
Match: Spred2 (sprouty-related EVH1 domain containing 2 [Source:MGI Symbol;Acc:MGI:2150019])

HSP 1 Score: 62.7734 bits (151), Expect = 2.523e-11
Identity = 39/116 (33.62%), Postives = 52/116 (44.83%), Query Frame = 3 
Query:  111 KRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            ++ + +   C YC  M+    N +G C +APD     I  VSCM CA    Y    D +GDY DP         +  R +    LS + PC+CCY PL            CGGKH+
Sbjct:  319 RKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHK 434
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Mouse
Match: Spred3 (sprouty-related EVH1 domain containing 3 [Source:MGI Symbol;Acc:MGI:2142186])

HSP 1 Score: 50.447 bits (119), Expect = 4.141e-7
Identity = 40/115 (34.78%), Postives = 59/115 (51.30%), Query Frame = 3 
Query:  126 DSYVCTYCFQMYADSRNVKGS-CSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPN-WRDRIKWYLLSLVLPCLCCYGPLVRLQH--SAR-KLSLCGGKHE 455
            ++  C +C  ++    + +G  C+EAPDP   ++  +SC+ CA+   Y    D +GD+ DP       P           LSL +PCLCCY PL R  H  +AR   + CGG+HE
Sbjct:  291 EAARCVHCRALFRRRADGRGGRCAEAPDPGRLLVRRLSCLWCAESLLYHCLSDAEGDFSDPCACEPGHPRPAARWAALAALSLAVPCLCCYAPL-RACHWVAARCGCAGCGGRHE 404
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. UniProt/SwissProt
Match: sp|Q66JG9|SPRE1_XENTR (Sprouty-related, EVH1 domain-containing protein 1 OS=Xenopus tropicalis OX=8364 GN=spred1 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.041e-11
Identity = 41/107 (38.32%), Postives = 53/107 (49.53%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC + +    N +G C +APDP ++ I  VSCM CA+   Y    D +GDY DP        N   R +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  297 CVYCQERFNHEENGRGKCQDAPDPIQRCIYQVSCMLCAESMLYHCMSDSEGDYSDPCSCDASDENLCLRWLALITLSFIAPCMCCYLPLRACHHCGEMCGCCGGKHK 403
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. UniProt/SwissProt
Match: sp|Q3C2P8|SPRE2_RAT (Sprouty-related, EVH1 domain-containing protein 2 OS=Rattus norvegicus OX=10116 GN=Spred2 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.209e-11
Identity = 40/119 (33.61%), Postives = 54/119 (45.38%), Query Frame = 3 
Query:  111 KRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP----WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            ++ + +   C YC  M+    N +G C +APD     I  VSCM CA    Y    D +GDY DP      + +    W   +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  292 RKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRW---MALIALSFLAPCMCCYLPLRACHHCGVMCRCCGGKHK 407
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. UniProt/SwissProt
Match: sp|Q7Z699|SPRE1_HUMAN (Sprouty-related, EVH1 domain-containing protein 1 OS=Homo sapiens OX=9606 GN=SPRED1 PE=1 SV=2)

HSP 1 Score: 65.4698 bits (158), Expect = 2.350e-11
Identity = 34/92 (36.96%), Postives = 49/92 (53.26%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP----WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPL 401
            C YC + +    NV+G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP      + +    W   +    LS ++PC+CCY PL
Sbjct:  335 CVYCQERFNHEENVRGKCQDAPDPIKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRW---LALVALSFIVPCMCCYVPL 423
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. UniProt/SwissProt
Match: sp|Q5RDN2|SPRE2_PONAB (Sprouty-related, EVH1 domain-containing protein 2 OS=Pongo abelii OX=9601 GN=SPRED2 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 5.361e-11
Identity = 40/107 (37.38%), Postives = 49/107 (45.79%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC  M+    N +G C +APD     I  VSCM CA    Y    D +GDY DP         +  R +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  309 CVYCRDMFNHEENRRGHCQDAPDSVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACYHCGVMCRCCGGKHK 415
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. UniProt/SwissProt
Match: sp|Q7Z698|SPRE2_HUMAN (Sprouty-related, EVH1 domain-containing protein 2 OS=Homo sapiens OX=9606 GN=SPRED2 PE=1 SV=2)

HSP 1 Score: 64.3142 bits (155), Expect = 5.569e-11
Identity = 40/110 (36.36%), Postives = 50/110 (45.45%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP----WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC  M+    N +G C +APD     I  VSCM CA    Y    D +GDY DP      + +    W   +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  309 CVYCRDMFNHEENRRGHCQDAPDSVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRW---MALIALSFLAPCMCCYLPLRACYHCGVMCRCCGGKHK 415
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. TrEMBL
Match: R7UND1 (WH1 domain-containing protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_34948 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 9.594e-14
Identity = 42/107 (39.25%), Postives = 56/107 (52.34%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWL-NTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC +M+    N +G C +APD   + I+ VSC+CCA+   Y    D DGDY  P + ++    N R      LLSL++PCL CY PL            CGGKH+
Sbjct:  319 CVYCHEMFVHEDNQRGRCEDAPDRGAQCIERVSCICCARGMLYHCMADADGDYGHPCVCDSSDEGNCRKWTALGLLSLIVPCLWCYAPLTACHRLGVLCGCCGGKHK 425
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. TrEMBL
Match: A0A3B4AR98 (Sprouty related EVH1 domain containing 1 OS=Periophthalmus magnuspinnatus OX=409849 PE=4 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.040e-13
Identity = 45/128 (35.16%), Postives = 62/128 (48.44%), Query Frame = 3 
Query:   75 KKLMSVNKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            K L S +K    +R   +   C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP         +  R +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  232 KYLFSDSKKSRRRREDGERSRCIYCREMFNHEDNRRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDEQFCLRWLALVALSFIAPCMCCYLPLRACHHCGEACRCCGGKHK 359
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. TrEMBL
Match: A0A060WY42 (KBD domain-containing protein OS=Oncorhynchus mykiss OX=8022 GN=GSONMT00011927001 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.729e-13
Identity = 40/107 (37.38%), Postives = 54/107 (50.47%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP         +  R +    LS + PC+CCY PL    H       CGGKH+
Sbjct:   81 CIYCREMFNHEDNQRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDTADEQFCLRWLALTALSFIAPCMCCYLPLRACHHCGEACRCCGGKHK 187
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. TrEMBL
Match: A0A3B3QJ92 (Sprouty related EVH1 domain containing 1 OS=Paramormyrops kingsleyae OX=1676925 PE=4 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.975e-13
Identity = 45/125 (36.00%), Postives = 61/125 (48.80%), Query Frame = 3 
Query:   84 MSVNKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            +   K    +R   +   CTYC +M+    N +G C +APDP  + I  VSCM CA+   Y    D +GD+ DP        ++  R +    LSLV PCLCCY PL    H       CGG+H+
Sbjct:  283 LKTTKASRRRREDGERSRCTYCREMFNHEDNRRGQCPDAPDPVRQCIHRVSCMLCAESMLYHCMSDSEGDFSDPCSCDPSEEHFCLRWMALAALSLVAPCLCCYLPLRACYHCGEVCRCCGGRHK 407
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. TrEMBL
Match: A0A3B5KYN1 (Sprouty related EVH1 domain containing 1 OS=Xiphophorus couchianus OX=32473 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.761e-13
Identity = 40/107 (37.38%), Postives = 54/107 (50.47%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP         +  R +    LS + PC+CCY PL    H       CGGKH+
Sbjct:   82 CIYCREMFNHEDNWRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDEQFCLRWLALLALSFIAPCMCCYLPLRACHHCGEACHCCGGKHK 188
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Cavefish
Match: spred1 (sprouty related EVH1 domain containing 1 [Source:NCBI gene;Acc:103044678])

HSP 1 Score: 76.6406 bits (187), Expect = 3.373e-16
Identity = 43/122 (35.25%), Postives = 61/122 (50.00%), Query Frame = 3 
Query:   93 NKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP-WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            +K    +R   +   C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP   +T +       +    LSL+ PC+CCY PL    H       CGGKH+
Sbjct:  267 SKKSRRRREDGERSRCIYCREMFNHEDNWRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSEEQFCLRWLALLGLSLIAPCMCCYPPLRACHHCGEACHCCGGKHK 388
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Cavefish
Match: spred1 (sprouty related EVH1 domain containing 1 [Source:NCBI gene;Acc:103044678])

HSP 1 Score: 76.2554 bits (186), Expect = 3.533e-16
Identity = 43/122 (35.25%), Postives = 61/122 (50.00%), Query Frame = 3 
Query:   93 NKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP-WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            +K    +R   +   C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP   +T +       +    LSL+ PC+CCY PL    H       CGGKH+
Sbjct:  255 SKKSRRRREDGERSRCIYCREMFNHEDNWRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSEEQFCLRWLALLGLSLIAPCMCCYPPLRACHHCGEACHCCGGKHK 376
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Cavefish
Match: spred1 (sprouty related EVH1 domain containing 1 [Source:NCBI gene;Acc:103044678])

HSP 1 Score: 76.2554 bits (186), Expect = 4.625e-16
Identity = 43/122 (35.25%), Postives = 61/122 (50.00%), Query Frame = 3 
Query:   93 NKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP-WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            +K    +R   +   C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP   +T +       +    LSL+ PC+CCY PL    H       CGGKH+
Sbjct:  288 SKKSRRRREDGERSRCIYCREMFNHEDNWRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSEEQFCLRWLALLGLSLIAPCMCCYPPLRACHHCGEACHCCGGKHK 409
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Cavefish
Match: spred2a (sprouty related EVH1 domain containing 2a [Source:ZFIN;Acc:ZDB-GENE-110830-2])

HSP 1 Score: 69.3218 bits (168), Expect = 1.139e-13
Identity = 41/107 (38.32%), Postives = 55/107 (51.40%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKWYL-LSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC  +++ ++N +G C EAPDP +  I  VS M CA    Y    D +GDY DP        ++  R    L LSL+ PC+CCY PL      A     CGG+H+
Sbjct:  296 CVYCRDVFSPTQNRRGQCQEAPDPVDTCIRRVSFMWCADSLLYHCMSDPEGDYSDPCSCDPSEEHFCLRWTALLGLSLIAPCMCCYAPLRACHRCAIACHCCGGRHK 402
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Cavefish
Match: spred2b (sprouty-related, EVH1 domain-containing protein 2-like [Source:NCBI gene;Acc:103032023])

HSP 1 Score: 53.9138 bits (128), Expect = 2.119e-8
Identity = 36/100 (36.00%), Postives = 50/100 (50.00%), Query Frame = 3 
Query:  111 KRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKW---YLLSLVLPCLCCYGPL 401
            ++   +   C YC  M+    N +G C +APDP    +  VS M CA    Y    D +GDY DP   +  + + R  ++W     LSL+ PCLCCY PL
Sbjct:  301 RKEDGERSRCVYCRDMFNHEENRRGQCHDAPDPIRTCVHRVSFMWCADSLLYHCMSDPEGDYSDPC--SCDTGDERFCLRWLALLGLSLLAPCLCCYPPL 398
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Sea Lamprey
Match: SPRED2 (sprouty related EVH1 domain containing 2 [Source:HGNC Symbol;Acc:HGNC:17722])

HSP 1 Score: 64.6994 bits (156), Expect = 8.944e-13
Identity = 33/91 (36.26%), Postives = 49/91 (53.85%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKW---YLLSLVLPCLCCYGPL 401
            C YC   +    N +G C +APDP+ + +  +SCMC A+   Y    D +G+Y DP  +     + R  ++W     LSL++PCLC Y PL
Sbjct:  259 CKYCRAAFDPPGNRRGGCPDAPDPARRCVTALSCMCAAESMLYHCMADPEGNYSDP-CSCETGGHERFGLRWAALVALSLLVPCLCLYPPL 348
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Sea Lamprey
Match: SPRED2 (sprouty related EVH1 domain containing 2 [Source:HGNC Symbol;Acc:HGNC:17722])

HSP 1 Score: 49.6766 bits (117), Expect = 1.122e-7
Identity = 31/98 (31.63%), Postives = 52/98 (53.06%), Query Frame = 3 
Query:  120 SADSYVCTYCFQMYADSRNVKGS-CSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKWYLL---SLVLPCLCCYGPL 401
            S +   C YC +++  SR  +   C++APD +   ++  SC+  A+   Y    D +GD+ DP  +     + R   +W++L   SL+ PC+CCY PL
Sbjct:  298 SGERARCIYCGELFDPSRPGRARRCADAPDRARVCVERASCLWLAETVLYHCMSDPEGDFSDP-CSCDGDGDGRVCQRWFVLAGLSLLAPCMCCYLPL 394
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Medaka
Match: spred1 (sprouty related EVH1 domain containing 1 [Source:NCBI gene;Acc:101162967])

HSP 1 Score: 73.559 bits (179), Expect = 3.883e-15
Identity = 40/107 (37.38%), Postives = 54/107 (50.47%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP         +  R +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  310 CIYCREMFNHEDNWRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDEQFCLRWLALVALSFIAPCMCCYLPLRACHHCGEACHCCGGKHK 416
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Medaka
Match: spred1 (sprouty related EVH1 domain containing 1 [Source:NCBI gene;Acc:101162967])

HSP 1 Score: 73.1738 bits (178), Expect = 4.702e-15
Identity = 40/107 (37.38%), Postives = 54/107 (50.47%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP         +  R +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  334 CIYCREMFNHEDNWRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDEQFCLRWLALVALSFIAPCMCCYLPLRACHHCGEACHCCGGKHK 440
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Medaka
Match: spred1 (sprouty related EVH1 domain containing 1 [Source:NCBI gene;Acc:101162967])

HSP 1 Score: 73.1738 bits (178), Expect = 6.402e-15
Identity = 40/107 (37.38%), Postives = 54/107 (50.47%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR-IKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHE 455
            C YC +M+    N +G C +APDP ++ I  VSCM CA+   Y    D +GD+ DP         +  R +    LS + PC+CCY PL    H       CGGKH+
Sbjct:  433 CIYCREMFNHEDNWRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDEQFCLRWLALVALSFIAPCMCCYLPLRACHHCGEACHCCGGKHK 539
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Medaka
Match: spred2a (sprouty-related, EVH1 domain-containing protein 2-like [Source:NCBI gene;Acc:105358735])

HSP 1 Score: 67.0106 bits (162), Expect = 7.693e-13
Identity = 44/120 (36.67%), Postives = 57/120 (47.50%), Query Frame = 3 
Query:  123 ADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDR---IKWYL---LSLVLPCLCCYGPLVRLQHSARKLSLCGGKHEPVT 464
            A+   C YC  M+    N +G C EAPDP    I  VS + CA    Y    D +G+Y DP      S + RD    ++W     LSL+ PC+CCYGPL            CGGKH+  +
Sbjct:  316 AEHLRCVYCQDMFRLEENWRGRCQEAPDPVRTCIRRVSFIWCADSLLYHCMSDPEGEYFDPC-----SCDTRDEHFCLRWAALVGLSLLAPCMCCYGPLRACHRCGVACRCCGGKHKAAS 430
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Medaka
Match: SPRED2 (sprouty related EVH1 domain containing 2 [Source:NCBI gene;Acc:101162704])

HSP 1 Score: 62.7734 bits (151), Expect = 1.982e-11
Identity = 40/117 (34.19%), Postives = 52/117 (44.44%), Query Frame = 3 
Query:  138 CTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDP--------WLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHEPVT 464
            C +C + +  S N +G C +APDP    I  +SCM  A    Y    D +GDY DP            R    W   +    LSLV PCLC Y PL    H       CGG+H+ ++
Sbjct:  351 CEHCGESFFISNNRRGRCQDAPDPVRACIQRISCMWLADTMLYHCMSDPEGDYSDPCSCDGGESGGGGRVGTRW---LALLGLSLVAPCLCLYPPLHACHHVGLVCGCCGGRHKAMS 464
[Close]
BLAST of Sprouty related EVH1 domain containing 2a vs. Planmine SMEST
Match: SMESG000045033.1 (SMESG000045033.1)

HSP 1 Score: 266.544 bits (680), Expect = 1.558e-92
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 3 
Query:   84 MSVNKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHEPVTV 467
            MSVNKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHEPVTV
Sbjct:    1 MSVNKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIVSCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKWYLLSLVLPCLCCYGPLVRLQHSARKLSLCGGKHEPVTV 128
The following BLAST results are available for this feature:
BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SPRED14.893e-1236.96sprouty related EVH1 domain containing 1 [Source:H... [more]
SPRED21.001e-1137.38sprouty related EVH1 domain containing 2 [Source:H... [more]
SPRED21.014e-1136.36sprouty related EVH1 domain containing 2 [Source:H... [more]
SPRED21.160e-1136.36sprouty related EVH1 domain containing 2 [Source:H... [more]
SPRED35.401e-734.78sprouty related EVH1 domain containing 3 [Source:H... [more]
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BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Spred1.447e-932.11gene:FBgn0020767 transcript:FBtr0087468[more]
Spred1.454e-932.11gene:FBgn0020767 transcript:FBtr0329879[more]
Spred2.266e-931.09gene:FBgn0020767 transcript:FBtr0329880[more]
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BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spred17.762e-1433.80sprouty related EVH1 domain containing 1 [Source:Z... [more]
spred15.187e-1333.80sprouty related EVH1 domain containing 1 [Source:Z... [more]
spred15.521e-1333.80sprouty related EVH1 domain containing 1 [Source:Z... [more]
spred2a1.827e-1239.34sprouty related EVH1 domain containing 2a [Source:... [more]
spred2a2.407e-1240.37sprouty related EVH1 domain containing 2a [Source:... [more]
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BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
spred11.965e-1238.32sprouty related, EVH1 domain containing 1 [Source:... [more]
gpat31.595e-1137.36glycerol-3-phosphate acyltransferase 3 [Source:Xen... [more]
spred21.272e-733.00sprouty related, EVH1 domain containing 2 [Source:... [more]
spred21.632e-733.00sprouty related, EVH1 domain containing 2 [Source:... [more]
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BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Spred18.774e-1235.87sprouty protein with EVH-1 domain 1, related seque... [more]
Spred22.179e-1133.62sprouty-related EVH1 domain containing 2 [Source:M... [more]
Spred22.523e-1133.62sprouty-related EVH1 domain containing 2 [Source:M... [more]
Spred34.141e-734.78sprouty-related EVH1 domain containing 3 [Source:M... [more]
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BLAST of Sprouty related EVH1 domain containing 2a vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q66JG9|SPRE1_XENTR1.041e-1138.32Sprouty-related, EVH1 domain-containing protein 1 ... [more]
sp|Q3C2P8|SPRE2_RAT1.209e-1133.61Sprouty-related, EVH1 domain-containing protein 2 ... [more]
sp|Q7Z699|SPRE1_HUMAN2.350e-1136.96Sprouty-related, EVH1 domain-containing protein 1 ... [more]
sp|Q5RDN2|SPRE2_PONAB5.361e-1137.38Sprouty-related, EVH1 domain-containing protein 2 ... [more]
sp|Q7Z698|SPRE2_HUMAN5.569e-1136.36Sprouty-related, EVH1 domain-containing protein 2 ... [more]
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BLAST of Sprouty related EVH1 domain containing 2a vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R7UND19.594e-1439.25WH1 domain-containing protein (Fragment) OS=Capite... [more]
A0A3B4AR981.040e-1335.16Sprouty related EVH1 domain containing 1 OS=Periop... [more]
A0A060WY421.729e-1337.38KBD domain-containing protein OS=Oncorhynchus myki... [more]
A0A3B3QJ921.975e-1336.00Sprouty related EVH1 domain containing 1 OS=Paramo... [more]
A0A3B5KYN12.761e-1337.38Sprouty related EVH1 domain containing 1 OS=Xiphop... [more]
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BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spred13.373e-1635.25sprouty related EVH1 domain containing 1 [Source:N... [more]
spred13.533e-1635.25sprouty related EVH1 domain containing 1 [Source:N... [more]
spred14.625e-1635.25sprouty related EVH1 domain containing 1 [Source:N... [more]
spred2a1.139e-1338.32sprouty related EVH1 domain containing 2a [Source:... [more]
spred2b2.119e-836.00sprouty-related, EVH1 domain-containing protein 2-... [more]
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BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
SPRED28.944e-1336.26sprouty related EVH1 domain containing 2 [Source:H... [more]
SPRED21.122e-731.63sprouty related EVH1 domain containing 2 [Source:H... [more]
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BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sprouty related EVH1 domain containing 2a vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spred13.883e-1537.38sprouty related EVH1 domain containing 1 [Source:N... [more]
spred14.702e-1537.38sprouty related EVH1 domain containing 1 [Source:N... [more]
spred16.402e-1537.38sprouty related EVH1 domain containing 1 [Source:N... [more]
spred2a7.693e-1336.67sprouty-related, EVH1 domain-containing protein 2-... [more]
SPRED21.982e-1134.19sprouty related EVH1 domain containing 2 [Source:N... [more]
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BLAST of Sprouty related EVH1 domain containing 2a vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000045033.11.558e-92100.00SMESG000045033.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035248 ID=SMED30035248|Name=Sprouty related EVH1 domain containing 2a|organism=Schmidtea mediterranea sexual|type=transcript|length=606bp
ATTAGTTGGCCATTCATTCCGTTTAAATACTACACAAATTCCTCAATGAA
TTACAAATTAGATTTTTTATTGAGAAAAAACTGATGAGTGTGAATAAAGA
CATGCTGATGAAAAGAGTTTCTGCGGATTCGTACGTTTGTACGTATTGTT
TTCAAATGTACGCCGACAGTCGAAACGTCAAAGGCAGTTGTTCAGAAGCT
CCAGATCCATCAGAGAAAGTCATTGATATTGTCAGTTGTATGTGTTGCGC
GAAATTTTGCTGGTATTTCATCACAGTCGACGATGATGGAGATTACGAAG
ATCCTTGGCTTAACACCAGGCAGTCTCCGAATTGGAGAGACAGAATTAAA
TGGTATTTATTGTCGCTAGTTCTTCCGTGTCTTTGTTGTTATGGACCTCT
CGTTAGACTTCAACATTCCGCAAGAAAGCTTTCATTGTGCGGCGGTAAAC
ACGAACCAGTAACTGTATAAATGGTTATCATTAACTTTTCATATTTACAG
CATTCGTTTTTCAATATTACTGACCAATTAATTATCTTATAAATGTAAAT
AAGGAATATATATTTTTTACTGGAAATATAATATATGTTTTTCTCTCTAA
AAAAAA
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protein sequence of SMED30035248-orf-1

>SMED30035248-orf-1 ID=SMED30035248-orf-1|Name=SMED30035248-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=129bp
MSVNKDMLMKRVSADSYVCTYCFQMYADSRNVKGSCSEAPDPSEKVIDIV
SCMCCAKFCWYFITVDDDGDYEDPWLNTRQSPNWRDRIKWYLLSLVLPCL
CCYGPLVRLQHSARKLSLCGGKHEPVTV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR007875Sprouty
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0009966regulation of signal transduction
GO:0007275multicellular organism development
GO:0010923negative regulation of phosphatase activity
Vocabulary: molecular function
TermDefinition
GO:0019902phosphatase binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007875SproutyPFAMPF05210Sproutycoord: 19..119
e-value: 8.1E-16
score: 58.3
NoneNo IPR availablePANTHERPTHR11202VASODILATOR-STIMULATED PHOSPHOPROTEIN/SPROUTY PROTEIN EVH1 DOMAIN CONTAINING PROTEIN 3coord: 10..125
NoneNo IPR availablePANTHERPTHR11202:SF3SPROUTY-RELATED PROTEIN WITH EVH-1 DOMAIN, ISOFORM Ccoord: 10..125