SMED30035230
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035230 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of SMED30035230 vs. Ensembl Human
Match: DCLK3 (doublecortin like kinase 3 [Source:HGNC Symbol;Acc:HGNC:19005]) HSP 1 Score: 283.878 bits (725), Expect = 2.092e-84 Identity = 137/300 (45.67%), Postives = 200/300 (66.67%), Query Frame = -2
Query: 278 SRPSTALPEKVFTKNKRR-GIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLAT-ILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGT 1171
+RP PE+ + R GII A+ Y G+ IG+G++AVV+E H T + A+K+ DK RL DMVD E+ I ++ +HPNI +L+ YET+ E+++++E V+GGDLFD+I+ +++F E A+++I DL AL ++H +++VHRD+KPEN+L+ + ++KL DFGLA ++R VCGTPTY+APEIL EKGYG EVD WA GVILYIL+ GFPPFR+P+R Q EL++ I G +++ +W+NIS A+ L+ LLV P++RYTA V+ W+ T G T
Sbjct: 327 TRPEENKPERPSGRKPRPMGII-----AANVEKHYETGRVIGDGNFAVVKECRHRETRQAYAMKIIDKSRLKGKEDMVDSEILIIQSLSHPNIVKLHEVYETDMEIYLILEYVQGGDLFDAIIESVKFPEPDAALMIMDLCKALVHMHDKSIVHRDLKPENLLVQRNEDKSTTLKLADFGLAKHVVR--PIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQLGHFEFLPPYWDNISDAAKDLVSRLLVVDPKKRYTAHQVLQHPWIETAGKT 619
BLAST of SMED30035230 vs. Ensembl Human
Match: DCLK3 (doublecortin like kinase 3 [Source:HGNC Symbol;Acc:HGNC:19005]) HSP 1 Score: 283.878 bits (725), Expect = 6.369e-83 Identity = 137/300 (45.67%), Postives = 200/300 (66.67%), Query Frame = -2
Query: 278 SRPSTALPEKVFTKNKR-RGIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLAT-ILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGT 1171
+RP PE+ + R GII A+ Y G+ IG+G++AVV+E H T + A+K+ DK RL DMVD E+ I ++ +HPNI +L+ YET+ E+++++E V+GGDLFD+I+ +++F E A+++I DL AL ++H +++VHRD+KPEN+L+ + ++KL DFGLA ++R VCGTPTY+APEIL EKGYG EVD WA GVILYIL+ GFPPFR+P+R Q EL++ I G +++ +W+NIS A+ L+ LLV P++RYTA V+ W+ T G T
Sbjct: 496 TRPEENKPERPSGRKPRPMGII-----AANVEKHYETGRVIGDGNFAVVKECRHRETRQAYAMKIIDKSRLKGKEDMVDSEILIIQSLSHPNIVKLHEVYETDMEIYLILEYVQGGDLFDAIIESVKFPEPDAALMIMDLCKALVHMHDKSIVHRDLKPENLLVQRNEDKSTTLKLADFGLAKHVVR--PIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQLGHFEFLPPYWDNISDAAKDLVSRLLVVDPKKRYTAHQVLQHPWIETAGKT 788 HSP 2 Score: 69.707 bits (169), Expect = 1.799e-11 Identity = 44/121 (36.36%), Postives = 71/121 (58.68%), Query Frame = -2
Query: 1763 LNDEHSHDSFSGSKMITVVR-AGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYK-ERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKEGLSRKIVKEILEDIYYPNNAEARSMLEY 2119
L+ H +S +++TVV+ G PR I L+ R + + +L +IS++L +K +RV KLFNL G EI D F+ D FIA+GKE L+ K ++ +E++ YPN A A ++ ++
Sbjct: 82 LHSAHPENSPLKPRVVTVVKLGGQRPRKIT-LLLNRRSVQTFEQLLADISEALGSPRWKNDRVRKLFNLKGREIRSVSDFFREGDAFIAMGKEPLTLKSIQVAVEEL-YPNKARALTLAQH 200
BLAST of SMED30035230 vs. Ensembl Human
Match: DCLK2 (doublecortin like kinase 2 [Source:HGNC Symbol;Acc:HGNC:19002]) HSP 1 Score: 273.092 bits (697), Expect = 4.794e-80 Identity = 127/267 (47.57%), Postives = 181/267 (67.79%), Query Frame = -2
Query: 296 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1096
E+S L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV I R HPNI L ET E+F+VMELVKGGDLFD+I + +++E S ++++LA+AL YLH ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + R TA ++ WV
Sbjct: 385 ESSTLLEKYKIGKVIGDGNFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWV 650 HSP 2 Score: 52.373 bits (124), Expect = 3.277e-6 Identity = 28/82 (34.15%), Postives = 47/82 (57.32%), Query Frame = -2
Query: 1847 FSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2092
F K++TV+R+G PR +R L+ + + VLT+I++++ S V +L L G+++ +D F +DVFIA G E
Sbjct: 193 FIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDS--GVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPE 272
BLAST of SMED30035230 vs. Ensembl Human
Match: DCLK2 (doublecortin like kinase 2 [Source:HGNC Symbol;Acc:HGNC:19002]) HSP 1 Score: 273.092 bits (697), Expect = 7.259e-80 Identity = 127/267 (47.57%), Postives = 181/267 (67.79%), Query Frame = -2
Query: 296 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1096
E+S L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV I R HPNI L ET E+F+VMELVKGGDLFD+I + +++E S ++++LA+AL YLH ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + R TA ++ WV
Sbjct: 402 ESSTLLEKYKIGKVIGDGNFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWV 667 HSP 2 Score: 52.373 bits (124), Expect = 3.177e-6 Identity = 28/82 (34.15%), Postives = 47/82 (57.32%), Query Frame = -2
Query: 1847 FSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2092
F K++TV+R+G PR +R L+ + + VLT+I++++ S V +L L G+++ +D F +DVFIA G E
Sbjct: 193 FIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDS--GVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPE 272
BLAST of SMED30035230 vs. Ensembl Human
Match: DCLK2 (doublecortin like kinase 2 [Source:HGNC Symbol;Acc:HGNC:19002]) HSP 1 Score: 272.707 bits (696), Expect = 2.554e-79 Identity = 127/267 (47.57%), Postives = 181/267 (67.79%), Query Frame = -2
Query: 296 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1096
E+S L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV I R HPNI L ET E+F+VMELVKGGDLFD+I + +++E S ++++LA+AL YLH ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + R TA ++ WV
Sbjct: 385 ESSTLLEKYKIGKVIGDGNFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWV 650 HSP 2 Score: 52.373 bits (124), Expect = 3.399e-6 Identity = 28/82 (34.15%), Postives = 47/82 (57.32%), Query Frame = -2
Query: 1847 FSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2092
F K++TV+R+G PR +R L+ + + VLT+I++++ S V +L L G+++ +D F +DVFIA G E
Sbjct: 193 FIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDS--GVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPE 272
BLAST of SMED30035230 vs. Ensembl Celegans
Match: unc-43 (Calcium/calmodulin-dependent protein kinase type II [Source:UniProtKB/Swiss-Prot;Acc:O62305]) HSP 1 Score: 205.682 bits (522), Expect = 8.028e-60 Identity = 110/290 (37.93%), Postives = 168/290 (57.93%), Query Frame = -2
Query: 242 IGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDM--VDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQA-HLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDS 1102
+ ++ F Y + +++G+G ++VVR H TG E A K+ + K+L+ A D ++ E I R HPNI RL+ + + E+ ++V +LV GG+LF+ IV +SE AS I + ++ Y HS +VHRD+KPEN+L++ G ++KL DFGLA + ++A H GTP Y++PE+L++ Y VD WA GVILYIL+ G+PPF D Q LY I G YDYP W+ ++ EA+ LID +L +P++R TA + W+ +H +D
Sbjct: 2 MNASTKFSDNYDVKEELGKGAFSVVRRCVHKTTGLEFAAKIINTKKLS-ARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESIAYCHSNGIVHRDLKPENLLLASKAKG-AAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKDPYSKPVDIWACGVILYILLVGYPPFW--DEDQHRLYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWICNRERVASAIHRQDTVDC 287
BLAST of SMED30035230 vs. Ensembl Celegans
Match: unc-43 (Calcium/calmodulin-dependent protein kinase type II [Source:UniProtKB/Swiss-Prot;Acc:O62305]) HSP 1 Score: 205.682 bits (522), Expect = 8.028e-60 Identity = 110/290 (37.93%), Postives = 168/290 (57.93%), Query Frame = -2
Query: 242 IGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDM--VDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQA-HLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDS 1102
+ ++ F Y + +++G+G ++VVR H TG E A K+ + K+L+ A D ++ E I R HPNI RL+ + + E+ ++V +LV GG+LF+ IV +SE AS I + ++ Y HS +VHRD+KPEN+L++ G ++KL DFGLA + ++A H GTP Y++PE+L++ Y VD WA GVILYIL+ G+PPF D Q LY I G YDYP W+ ++ EA+ LID +L +P++R TA + W+ +H +D
Sbjct: 2 MNASTKFSDNYDVKEELGKGAFSVVRRCVHKTTGLEFAAKIINTKKLS-ARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESIAYCHSNGIVHRDLKPENLLLASKAKG-AAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKDPYSKPVDIWACGVILYILLVGYPPFW--DEDQHRLYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWICNRERVASAIHRQDTVDC 287
BLAST of SMED30035230 vs. Ensembl Celegans
Match: unc-43 (Calcium/calmodulin-dependent protein kinase type II [Source:UniProtKB/Swiss-Prot;Acc:O62305]) HSP 1 Score: 205.682 bits (522), Expect = 8.028e-60 Identity = 110/290 (37.93%), Postives = 168/290 (57.93%), Query Frame = -2
Query: 242 IGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDM--VDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQA-HLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDS 1102
+ ++ F Y + +++G+G ++VVR H TG E A K+ + K+L+ A D ++ E I R HPNI RL+ + + E+ ++V +LV GG+LF+ IV +SE AS I + ++ Y HS +VHRD+KPEN+L++ G ++KL DFGLA + ++A H GTP Y++PE+L++ Y VD WA GVILYIL+ G+PPF D Q LY I G YDYP W+ ++ EA+ LID +L +P++R TA + W+ +H +D
Sbjct: 2 MNASTKFSDNYDVKEELGKGAFSVVRRCVHKTTGLEFAAKIINTKKLS-ARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESIAYCHSNGIVHRDLKPENLLLASKAKG-AAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKDPYSKPVDIWACGVILYILLVGYPPFW--DEDQHRLYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWICNRERVASAIHRQDTVDC 287
BLAST of SMED30035230 vs. Ensembl Celegans
Match: unc-43 (Calcium/calmodulin-dependent protein kinase type II [Source:UniProtKB/Swiss-Prot;Acc:O62305]) HSP 1 Score: 205.297 bits (521), Expect = 8.110e-60 Identity = 110/290 (37.93%), Postives = 168/290 (57.93%), Query Frame = -2
Query: 242 IGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDM--VDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQA-HLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDS 1102
+ ++ F Y + +++G+G ++VVR H TG E A K+ + K+L+ A D ++ E I R HPNI RL+ + + E+ ++V +LV GG+LF+ IV +SE AS I + ++ Y HS +VHRD+KPEN+L++ G ++KL DFGLA + ++A H GTP Y++PE+L++ Y VD WA GVILYIL+ G+PPF D Q LY I G YDYP W+ ++ EA+ LID +L +P++R TA + W+ +H +D
Sbjct: 2 MNASTKFSDNYDVKEELGKGAFSVVRRCVHKTTGLEFAAKIINTKKLS-ARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESIAYCHSNGIVHRDLKPENLLLASKAKG-AAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKDPYSKPVDIWACGVILYILLVGYPPFW--DEDQHRLYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWICNRERVASAIHRQDTVDC 287
BLAST of SMED30035230 vs. Ensembl Celegans
Match: unc-43 (Calcium/calmodulin-dependent protein kinase type II [Source:UniProtKB/Swiss-Prot;Acc:O62305]) HSP 1 Score: 205.297 bits (521), Expect = 8.110e-60 Identity = 110/290 (37.93%), Postives = 168/290 (57.93%), Query Frame = -2
Query: 242 IGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDM--VDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQA-HLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDS 1102
+ ++ F Y + +++G+G ++VVR H TG E A K+ + K+L+ A D ++ E I R HPNI RL+ + + E+ ++V +LV GG+LF+ IV +SE AS I + ++ Y HS +VHRD+KPEN+L++ G ++KL DFGLA + ++A H GTP Y++PE+L++ Y VD WA GVILYIL+ G+PPF D Q LY I G YDYP W+ ++ EA+ LID +L +P++R TA + W+ +H +D
Sbjct: 2 MNASTKFSDNYDVKEELGKGAFSVVRRCVHKTTGLEFAAKIINTKKLS-ARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESIAYCHSNGIVHRDLKPENLLLASKAKG-AAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKDPYSKPVDIWACGVILYILLVGYPPFW--DEDQHRLYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWICNRERVASAIHRQDTVDC 287
BLAST of SMED30035230 vs. Ensembl Fly
Match: CG17528 (gene:FBgn0261387 transcript:FBtr0111274) HSP 1 Score: 251.521 bits (641), Expect = 5.098e-72 Identity = 119/274 (43.43%), Postives = 174/274 (63.50%), Query Frame = -2
Query: 254 YRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRI-SIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTH 1072
Y +G+ IG+G++A+V + H TG A+K+ DK + +D EV + + NHP+I L + + +++V+E V GGDLFD+I + RFSE + ++I L +A+ YLHS +VHRDIKPEN+L+ D HG + +KL DFGLA + + + VCGTPTY+APEIL E GYG ++D WA G+ILYIL+ GFPPF PD QQ L+D I G+Y++P +W +I R LI ++L P R+T+ D++ SW + G +GN T+
Sbjct: 477 YSLGRIIGDGNFAIVFKIKHRQTGHSYALKIIDKNKCKGKEHYIDAEVRVMKKLNHPHIISLILSVDQNTNMYLVLEYVSGGDLFDAITQVTRFSENQSRIMIRHLGAAMTYLHSMGIVHRDIKPENLLVKLDEHGNVLELKLADFGLACEVN-DLLYAVCGTPTYVAPEILLEVGYGLKIDVWAAGIILYILLCGFPPFVAPDNQQEPLFDAIISGIYEFPDPYWSDIGDGVRDLIANMLQADPDVRFTSEDILDHSWTI---GNKGNECTTY 746 HSP 2 Score: 51.9878 bits (123), Expect = 2.455e-6 Identity = 28/79 (35.44%), Postives = 47/79 (59.49%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSL-VQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
+++T++R+G PR I+R L+ + N + VLT I+Q + + + Y V K+F L G ++ D F ++DVF A G E
Sbjct: 313 RIVTLIRSGTKPRRIMRLLLNKRNSPSFDHVLTAITQVVRLDTGY---VRKVFTLSGIPVVRLSDFFGSDDVFFAYGTE 388
BLAST of SMED30035230 vs. Ensembl Fly
Match: CG17528 (gene:FBgn0261387 transcript:FBtr0111277) HSP 1 Score: 251.521 bits (641), Expect = 5.098e-72 Identity = 119/274 (43.43%), Postives = 174/274 (63.50%), Query Frame = -2
Query: 254 YRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRI-SIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTH 1072
Y +G+ IG+G++A+V + H TG A+K+ DK + +D EV + + NHP+I L + + +++V+E V GGDLFD+I + RFSE + ++I L +A+ YLHS +VHRDIKPEN+L+ D HG + +KL DFGLA + + + VCGTPTY+APEIL E GYG ++D WA G+ILYIL+ GFPPF PD QQ L+D I G+Y++P +W +I R LI ++L P R+T+ D++ SW + G +GN T+
Sbjct: 477 YSLGRIIGDGNFAIVFKIKHRQTGHSYALKIIDKNKCKGKEHYIDAEVRVMKKLNHPHIISLILSVDQNTNMYLVLEYVSGGDLFDAITQVTRFSENQSRIMIRHLGAAMTYLHSMGIVHRDIKPENLLVKLDEHGNVLELKLADFGLACEVN-DLLYAVCGTPTYVAPEILLEVGYGLKIDVWAAGIILYILLCGFPPFVAPDNQQEPLFDAIISGIYEFPDPYWSDIGDGVRDLIANMLQADPDVRFTSEDILDHSWTI---GNKGNECTTY 746 HSP 2 Score: 51.9878 bits (123), Expect = 2.455e-6 Identity = 28/79 (35.44%), Postives = 47/79 (59.49%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSL-VQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
+++T++R+G PR I+R L+ + N + VLT I+Q + + + Y V K+F L G ++ D F ++DVF A G E
Sbjct: 313 RIVTLIRSGTKPRRIMRLLLNKRNSPSFDHVLTAITQVVRLDTGY---VRKVFTLSGIPVVRLSDFFGSDDVFFAYGTE 388
BLAST of SMED30035230 vs. Ensembl Fly
Match: CaMKI (gene:FBgn0016126 transcript:FBtr0333670) HSP 1 Score: 202.216 bits (513), Expect = 1.596e-57 Identity = 104/275 (37.82%), Postives = 161/275 (58.55%), Query Frame = -2
Query: 257 KYRMGKKIGEGHYAVVREG-SHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENIL-ISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDT 1075
KY + +G G ++ VR S + G A+K+ DKK L + +++E+ + R HPNI +L YE +++V++VMELV GG+LFD IV ++E+ AS +I + A+ Y+H + VVHRD+KPEN+L S D +I I +DFGL+ + CGTP Y+APE+L +K YG VD W++GVI YIL+ G+PPF D L+ I KG +++ +W+ IS A+ I +L+ +RYT + + +W+ + N+H T
Sbjct: 30 KYNLHGLLGTGAFSEVRLAESKDSPGEHFAVKIIDKKALKGKEESLENEIRVLRRLTHPNIVQLLETYEDKSKVYLVMELVTGGELFDRIVEKGSYTEKDASHLIRQILEAVDYMHEQGVVHRDLKPENLLYYSPDDDSKIMI--SDFGLSKMEDSGIMATACGTPGYVAPEVLAQKPYGKAVDVWSIGVISYILLCGYPPFY--DENDANLFAQILKGDFEFDSPYWDEISESAKHFIKNLMCVTVEKRYTCKQALGHAWISGNEASSRNIHGT 300
BLAST of SMED30035230 vs. Ensembl Fly
Match: CaMKII (gene:FBgn0264607 transcript:FBtr0333673) HSP 1 Score: 199.519 bits (506), Expect = 1.767e-55 Identity = 106/267 (39.70%), Postives = 163/267 (61.05%), Query Frame = -2
Query: 296 FLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDM--VDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGN-QAHL-VCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1084
F Y + +++G+G +++V+ +TG E A K+ + K+LT A D ++ E I R +HPNI RL+ + + E ++V +LV GG+LF+ IV +SE AS I + ++ + H VVHRD+KPEN+L++ G ++KL DFGLA ++G+ QA GTP Y++PE+L+++ YG VD WA GVILYIL+ G+PPF D Q LY I G YDYP W+ ++ EA+ LI+ +L +P +R TA + + W+
Sbjct: 10 FSDNYDIKEELGKGAFSIVKRCVQKSTGFEFAAKIINTKKLT-ARDFQKLEREARICRKLHHPNIVRLHDSIQEENYHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVNHCHQNGVVHRDLKPENLLLASKAKG-AAVKLADFGLAIEVQGDHQAWFGFAGTPGYLSPEVLKKEPYGKSVDIWACGVILYILLVGYPPFW--DEDQHRLYSQIKAGAYDYPSPEWDTVTPEAKNLINQMLTVNPNKRITAAEALKHPWI 272
BLAST of SMED30035230 vs. Ensembl Fly
Match: CaMKII (gene:FBgn0264607 transcript:FBtr0300377) HSP 1 Score: 199.519 bits (506), Expect = 1.904e-55 Identity = 106/267 (39.70%), Postives = 163/267 (61.05%), Query Frame = -2
Query: 296 FLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDM--VDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGN-QAHL-VCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1084
F Y + +++G+G +++V+ +TG E A K+ + K+LT A D ++ E I R +HPNI RL+ + + E ++V +LV GG+LF+ IV +SE AS I + ++ + H VVHRD+KPEN+L++ G ++KL DFGLA ++G+ QA GTP Y++PE+L+++ YG VD WA GVILYIL+ G+PPF D Q LY I G YDYP W+ ++ EA+ LI+ +L +P +R TA + + W+
Sbjct: 10 FSDNYDIKEELGKGAFSIVKRCVQKSTGFEFAAKIINTKKLT-ARDFQKLEREARICRKLHHPNIVRLHDSIQEENYHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVNHCHQNGVVHRDLKPENLLLASKAKG-AAVKLADFGLAIEVQGDHQAWFGFAGTPGYLSPEVLKKEPYGKSVDIWACGVILYILLVGYPPFW--DEDQHRLYSQIKAGAYDYPSPEWDTVTPEAKNLINQMLTVNPNKRITAAEALKHPWI 272
BLAST of SMED30035230 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170]) HSP 1 Score: 283.108 bits (723), Expect = 5.178e-84 Identity = 134/276 (48.55%), Postives = 188/276 (68.12%), Query Frame = -2
Query: 266 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNL 1093
AS L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV + R HPNI L +T AE+++VMELVKGGDLFD+I + +++E+ AS+++ DLA+AL YLH +VHRDIKPEN+L+ E +G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P FW+NIS A+ LI H+L + RYTA DV+ WV N+
Sbjct: 385 ASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNM 659 HSP 2 Score: 55.4546 bits (132), Expect = 2.483e-7 Identity = 28/78 (35.90%), Postives = 47/78 (60.26%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
K++TV+R+G PR +R L+ + + VLT+I+ ++ S V +L+ L G++I+ +D F +DVFIA G E
Sbjct: 191 KLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDS--GAVKRLYTLEGKQIICLQDFFGDDDVFIACGPE 266
BLAST of SMED30035230 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170]) HSP 1 Score: 283.108 bits (723), Expect = 7.851e-84 Identity = 134/276 (48.55%), Postives = 188/276 (68.12%), Query Frame = -2
Query: 266 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNL 1093
AS L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV + R HPNI L +T AE+++VMELVKGGDLFD+I + +++E+ AS+++ DLA+AL YLH +VHRDIKPEN+L+ E +G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P FW+NIS A+ LI H+L + RYTA DV+ WV N+
Sbjct: 394 ASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNM 668 HSP 2 Score: 55.4546 bits (132), Expect = 2.703e-7 Identity = 28/78 (35.90%), Postives = 47/78 (60.26%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
K++TV+R+G PR +R L+ + + VLT+I+ ++ S V +L+ L G++I+ +D F +DVFIA G E
Sbjct: 191 KLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDS--GAVKRLYTLEGKQIICLQDFFGDDDVFIACGPE 266
BLAST of SMED30035230 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170]) HSP 1 Score: 283.108 bits (723), Expect = 7.851e-84 Identity = 134/276 (48.55%), Postives = 188/276 (68.12%), Query Frame = -2
Query: 266 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNL 1093
AS L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV + R HPNI L +T AE+++VMELVKGGDLFD+I + +++E+ AS+++ DLA+AL YLH +VHRDIKPEN+L+ E +G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P FW+NIS A+ LI H+L + RYTA DV+ WV N+
Sbjct: 394 ASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNM 668 HSP 2 Score: 55.4546 bits (132), Expect = 2.703e-7 Identity = 28/78 (35.90%), Postives = 47/78 (60.26%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
K++TV+R+G PR +R L+ + + VLT+I+ ++ S V +L+ L G++I+ +D F +DVFIA G E
Sbjct: 191 KLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDS--GAVKRLYTLEGKQIICLQDFFGDDDVFIACGPE 266
BLAST of SMED30035230 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170]) HSP 1 Score: 283.108 bits (723), Expect = 1.035e-83 Identity = 134/276 (48.55%), Postives = 188/276 (68.12%), Query Frame = -2
Query: 266 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNL 1093
AS L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV + R HPNI L +T AE+++VMELVKGGDLFD+I + +++E+ AS+++ DLA+AL YLH +VHRDIKPEN+L+ E +G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P FW+NIS A+ LI H+L + RYTA DV+ WV N+
Sbjct: 413 ASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNM 687 HSP 2 Score: 55.4546 bits (132), Expect = 2.607e-7 Identity = 28/78 (35.90%), Postives = 47/78 (60.26%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
K++TV+R+G PR +R L+ + + VLT+I+ ++ S V +L+ L G++I+ +D F +DVFIA G E
Sbjct: 191 KLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDS--GAVKRLYTLEGKQIICLQDFFGDDDVFIACGPE 266
BLAST of SMED30035230 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170]) HSP 1 Score: 283.108 bits (723), Expect = 1.129e-83 Identity = 134/276 (48.55%), Postives = 188/276 (68.12%), Query Frame = -2
Query: 266 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNL 1093
AS L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV + R HPNI L +T AE+++VMELVKGGDLFD+I + +++E+ AS+++ DLA+AL YLH +VHRDIKPEN+L+ E +G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P FW+NIS A+ LI H+L + RYTA DV+ WV N+
Sbjct: 413 ASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNM 687 HSP 2 Score: 55.4546 bits (132), Expect = 2.777e-7 Identity = 28/78 (35.90%), Postives = 47/78 (60.26%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
K++TV+R+G PR +R L+ + + VLT+I+ ++ S V +L+ L G++I+ +D F +DVFIA G E
Sbjct: 191 KLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDS--GAVKRLYTLEGKQIICLQDFFGDDDVFIACGPE 266
BLAST of SMED30035230 vs. Ensembl Xenopus
Match: ovch1 (ovochymase 1 [Source:Xenbase;Acc:XB-GENE-6449526]) HSP 1 Score: 281.182 bits (718), Expect = 8.151e-83 Identity = 136/288 (47.22%), Postives = 197/288 (68.40%), Query Frame = -2
Query: 224 SKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRG-NLHD--THNIDSQSRSRR 1078
S Y +G+ IG+G++AVV+ +E A+K+ DK +L D++++EV I + +HPNI +L++ YETE E+++++E ++GGDLFD I +I+F+E A++++ DL AL Y+HS+++VHRD+KPEN+L+ + G S+KL DFGLA G VCGTPTY+APEIL EKGYG +VD WA GVILYIL+ GFPPFR+PDR Q EL+ I G Y++ +W++IS E + LI LLV +P RY+A+ V+ SWV + G T NL T NI+ R+R+
Sbjct: 427 SYYEIGRTIGDGNFAVVKVCRPWNENQEYAMKIIDKSKLRGKEDIIENEVRIIKHLSHPNIVKLFSDYETEKEIYLILEYIRGGDLFDVITESIKFTEHDAALMLIDLCEALKYIHSKHIVHRDLKPENLLVQHNPDGSKSLKLADFGLAIYATG-PIFTVCGTPTYVAPEILSEKGYGLKVDMWATGVILYILLCGFPPFRSPDRNQEELFQIIQSGEYEFLPPYWDHISEEVKDLISKLLVLNPEIRYSAKCVLQHSWVTSRGQTNSRNLQREVTVNIERHFRNRQ 713 HSP 2 Score: 67.3958 bits (163), Expect = 7.432e-11 Identity = 34/103 (33.01%), Postives = 62/103 (60.19%), Query Frame = -2
Query: 1802 HSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYK-ERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKEGLSRKIVKEILEDIY 2107
HS +S +++TVV+ G P I L+ + + + ++ +IS++L +K +RV KL+NL G+EI D F+ +D+FIA+G+E L+ K + + ++Y
Sbjct: 31 HSENSPVKPRIVTVVKPGSHPLKKITLLLNKRSVQTFEQLVADISEALGFPRWKNDRVRKLYNLRGKEIRSISDFFRGDDIFIALGREKLTTKCTESVFNELY 133
BLAST of SMED30035230 vs. Ensembl Xenopus
Match: DCLK2 (doublecortin like kinase 2 [Source:NCBI gene;Acc:100487296]) HSP 1 Score: 276.174 bits (705), Expect = 2.495e-81 Identity = 130/278 (46.76%), Postives = 191/278 (68.71%), Query Frame = -2
Query: 263 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLH 1096
+AS+ L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV I R HPNI L +T AE+++VMELVKGGDLFD+I + +++E AS ++++LASA+ YLH ++VHRDIKPEN+L+ E S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + ERYTA ++ WV + ++GN+
Sbjct: 373 DASIILEKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKAKCCGKEHLIENEVSILRQVKHPNIIMLIEEMDTTAELYLVMELVKGGDLFDAITSSTKYTERDASAMVYNLASAMKYLHGLHIVHRDIKPENLLVCEYPDKTKSLKLGDFGLATVVDG-PLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILIGKLEFPSPYWDNITDSAKELISCMLQVNVEERYTAEQILSHPWV-SDDASQGNMQ 648 HSP 2 Score: 53.1434 bits (126), Expect = 1.856e-6 Identity = 27/78 (34.62%), Postives = 46/78 (58.97%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
K++TV+R+G PR +R L+ + + VLT+I++++ S V +L L G+++ +D F +DVFIA G E
Sbjct: 197 KLVTVIRSGSKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDS--GVVKRLCTLDGKQVTCLQDFFGEDDVFIACGPE 272
BLAST of SMED30035230 vs. Ensembl Xenopus
Match: DCLK2 (doublecortin like kinase 2 [Source:NCBI gene;Acc:100487296]) HSP 1 Score: 276.174 bits (705), Expect = 3.358e-81 Identity = 130/278 (46.76%), Postives = 191/278 (68.71%), Query Frame = -2
Query: 263 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLH 1096
+AS+ L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV I R HPNI L +T AE+++VMELVKGGDLFD+I + +++E AS ++++LASA+ YLH ++VHRDIKPEN+L+ E S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + ERYTA ++ WV + ++GN+
Sbjct: 383 DASIILEKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKAKCCGKEHLIENEVSILRQVKHPNIIMLIEEMDTTAELYLVMELVKGGDLFDAITSSTKYTERDASAMVYNLASAMKYLHGLHIVHRDIKPENLLVCEYPDKTKSLKLGDFGLATVVDG-PLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILIGKLEFPSPYWDNITDSAKELISCMLQVNVEERYTAEQILSHPWV-SDDASQGNMQ 658 HSP 2 Score: 53.1434 bits (126), Expect = 1.884e-6 Identity = 27/78 (34.62%), Postives = 46/78 (58.97%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
K++TV+R+G PR +R L+ + + VLT+I++++ S V +L L G+++ +D F +DVFIA G E
Sbjct: 197 KLVTVIRSGSKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDS--GVVKRLCTLDGKQVTCLQDFFGEDDVFIACGPE 272
BLAST of SMED30035230 vs. Ensembl Xenopus
Match: DCLK2 (doublecortin like kinase 2 [Source:NCBI gene;Acc:100487296]) HSP 1 Score: 276.174 bits (705), Expect = 3.708e-81 Identity = 130/278 (46.76%), Postives = 191/278 (68.71%), Query Frame = -2
Query: 263 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLH 1096
+AS+ L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV I R HPNI L +T AE+++VMELVKGGDLFD+I + +++E AS ++++LASA+ YLH ++VHRDIKPEN+L+ E S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + ERYTA ++ WV + ++GN+
Sbjct: 390 DASIILEKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKAKCCGKEHLIENEVSILRQVKHPNIIMLIEEMDTTAELYLVMELVKGGDLFDAITSSTKYTERDASAMVYNLASAMKYLHGLHIVHRDIKPENLLVCEYPDKTKSLKLGDFGLATVVDG-PLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILIGKLEFPSPYWDNITDSAKELISCMLQVNVEERYTAEQILSHPWV-SDDASQGNMQ 665 HSP 2 Score: 53.1434 bits (126), Expect = 1.680e-6 Identity = 27/78 (34.62%), Postives = 46/78 (58.97%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
K++TV+R+G PR +R L+ + + VLT+I++++ S V +L L G+++ +D F +DVFIA G E
Sbjct: 197 KLVTVIRSGSKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDS--GVVKRLCTLDGKQVTCLQDFFGEDDVFIACGPE 272
BLAST of SMED30035230 vs. Ensembl Xenopus
Match: DCLK2 (doublecortin like kinase 2 [Source:NCBI gene;Acc:100487296]) HSP 1 Score: 276.174 bits (705), Expect = 3.738e-81 Identity = 130/278 (46.76%), Postives = 191/278 (68.71%), Query Frame = -2
Query: 263 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLH 1096
+AS+ L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV I R HPNI L +T AE+++VMELVKGGDLFD+I + +++E AS ++++LASA+ YLH ++VHRDIKPEN+L+ E S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + ERYTA ++ WV + ++GN+
Sbjct: 390 DASIILEKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKAKCCGKEHLIENEVSILRQVKHPNIIMLIEEMDTTAELYLVMELVKGGDLFDAITSSTKYTERDASAMVYNLASAMKYLHGLHIVHRDIKPENLLVCEYPDKTKSLKLGDFGLATVVDG-PLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILIGKLEFPSPYWDNITDSAKELISCMLQVNVEERYTAEQILSHPWV-SDDASQGNMQ 665 HSP 2 Score: 53.1434 bits (126), Expect = 1.692e-6 Identity = 27/78 (34.62%), Postives = 46/78 (58.97%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
K++TV+R+G PR +R L+ + + VLT+I++++ S V +L L G+++ +D F +DVFIA G E
Sbjct: 197 KLVTVIRSGSKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDS--GVVKRLCTLDGKQVTCLQDFFGEDDVFIACGPE 272
BLAST of SMED30035230 vs. Ensembl Mouse
Match: Dclk3 (doublecortin-like kinase 3 [Source:MGI Symbol;Acc:MGI:3039580]) HSP 1 Score: 283.493 bits (724), Expect = 3.696e-83 Identity = 138/300 (46.00%), Postives = 200/300 (66.67%), Query Frame = -2
Query: 278 PSRPSTALPEKVFTKNKRRGIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLAT-ILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGT 1174
P RPS P + +GII ++ Y +G IG+G++A V+E H T + A+K+ DK +L D+VD E+ I ++ +HPNI +L+ YETEAE++++ME V+GGDLFD+IV N++F E A+++I DL AL ++H +N+VHRD+KPEN+L+ + I++KL DFGLA ++R VCGTPTY+APEIL EKGYG EVD WA GVILYIL+ GFPPFR+P+R Q EL++ I G +++ +W+NIS A+ L+ +LL P++RYTA V+ W+ +G T
Sbjct: 492 PERPSGRKP-------RPKGII-----SADVEKHYDIGGVIGDGNFATVKECRHRETKQAYAMKMIDKSQLKGKEDIVDSEILIIQSLSHPNIVKLHEVYETEAEIYLIMEYVQGGDLFDAIVENVKFPEPEAAVMITDLCKALVHMHDKNIVHRDVKPENLLVQRNEDKSITLKLADFGLAKYVVR--PIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQVGQFEFLSPYWDNISDAAKDLVRNLLEVDPKKRYTAEQVLQHPWIEMVGHT 777 HSP 2 Score: 62.3882 bits (150), Expect = 1.815e-9 Identity = 44/137 (32.12%), Postives = 70/137 (51.09%), Query Frame = -2
Query: 1781 DKPVKSPRARQINQKVANLEASHLNDEHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYK-ERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKEGLSRKIVKEILEDIYYPNNAEA 2188
+KPV PR + + EHS +++TVV+ G P L+ R + + +L++IS++L +K +RV KLF L G E+ D F+ D FIA+GKE L+ K ++ +E++Y N A A
Sbjct: 64 EKPVLGPRGAVMPLFSPQSSLHSVRAEHSPLK---PRVVTVVKLGGQPLRKATLLLNRRSVQTFEQLLSDISEALGFPRWKNDRVRKLFTLKGREVKSVSDFFREGDAFIAMGKEPLTLKSIQLAMEELYPKNRALA 197
BLAST of SMED30035230 vs. Ensembl Mouse
Match: Dclk2 (doublecortin-like kinase 2 [Source:MGI Symbol;Acc:MGI:1918012]) HSP 1 Score: 270.781 bits (691), Expect = 3.356e-79 Identity = 127/267 (47.57%), Postives = 181/267 (67.79%), Query Frame = -2
Query: 296 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1096
E+ L KYR+GK IG+G++AVV+E TG+E A+K+ DK + ++++EV I R HPNI L ET ++F+VMELVKGGDLFD+I + +++E S ++++LA+AL YLHS ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + R TA +++ WV
Sbjct: 401 ESFPLLEKYRIGKVIGDGNFAVVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV 666 HSP 2 Score: 52.373 bits (124), Expect = 2.175e-6 Identity = 28/82 (34.15%), Postives = 47/82 (57.32%), Query Frame = -2
Query: 1847 FSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2092
F K++TV+R+G PR +R L+ + + VLT+I++++ S V +L L G+++ +D F +DVFIA G E
Sbjct: 192 FIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDS--GVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPE 271
BLAST of SMED30035230 vs. Ensembl Mouse
Match: Dclk2 (doublecortin-like kinase 2 [Source:MGI Symbol;Acc:MGI:1918012]) HSP 1 Score: 270.781 bits (691), Expect = 7.502e-79 Identity = 127/267 (47.57%), Postives = 181/267 (67.79%), Query Frame = -2
Query: 296 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1096
E+ L KYR+GK IG+G++AVV+E TG+E A+K+ DK + ++++EV I R HPNI L ET ++F+VMELVKGGDLFD+I + +++E S ++++LA+AL YLHS ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + R TA +++ WV
Sbjct: 385 ESFPLLEKYRIGKVIGDGNFAVVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV 650 HSP 2 Score: 52.7582 bits (125), Expect = 2.062e-6 Identity = 28/82 (34.15%), Postives = 47/82 (57.32%), Query Frame = -2
Query: 1847 FSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2092
F K++TV+R+G PR +R L+ + + VLT+I++++ S V +L L G+++ +D F +DVFIA G E
Sbjct: 192 FIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDS--GVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPE 271
BLAST of SMED30035230 vs. Ensembl Mouse
Match: Dclk2 (doublecortin-like kinase 2 [Source:MGI Symbol;Acc:MGI:1918012]) HSP 1 Score: 270.781 bits (691), Expect = 7.744e-79 Identity = 127/267 (47.57%), Postives = 181/267 (67.79%), Query Frame = -2
Query: 296 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1096
E+ L KYR+GK IG+G++AVV+E TG+E A+K+ DK + ++++EV I R HPNI L ET ++F+VMELVKGGDLFD+I + +++E S ++++LA+AL YLHS ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + R TA +++ WV
Sbjct: 384 ESFPLLEKYRIGKVIGDGNFAVVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV 649 HSP 2 Score: 52.7582 bits (125), Expect = 2.026e-6 Identity = 28/82 (34.15%), Postives = 47/82 (57.32%), Query Frame = -2
Query: 1847 FSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2092
F K++TV+R+G PR +R L+ + + VLT+I++++ S V +L L G+++ +D F +DVFIA G E
Sbjct: 192 FIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDS--GVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPE 271
BLAST of SMED30035230 vs. Ensembl Mouse
Match: Dclk1 (doublecortin-like kinase 1 [Source:MGI Symbol;Acc:MGI:1330861]) HSP 1 Score: 257.684 bits (657), Expect = 7.515e-78 Identity = 124/260 (47.69%), Postives = 175/260 (67.31%), Query Frame = -2
Query: 296 KYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1075
+Y++G+ IG+G++AVV+E +T RE A+K+ K + M+ +EV I R HPNI L + E+++VMELVKGGDLFD+I +++E AS ++++LASA+ YLHS N+VHRDIKPEN+L+ E G S+KL DFGLATI+ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR Q L+D I G D+P +W+N+S A+ LI+ +L+ + +R++A V+ WV
Sbjct: 82 RYKVGRTIGDGNFAVVKECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDG-PLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELINMMLLVNVDQRFSAVQVLEHPWV 340
BLAST of SMED30035230 vs. UniProt/SwissProt
Match: sp|Q9C098|DCLK3_HUMAN (Serine/threonine-protein kinase DCLK3 OS=Homo sapiens OX=9606 GN=DCLK3 PE=2 SV=2) HSP 1 Score: 283.878 bits (725), Expect = 1.005e-83 Identity = 137/300 (45.67%), Postives = 200/300 (66.67%), Query Frame = -2
Query: 278 SRPSTALPEKVFTKNKRR-GIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLAT-ILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGT 1171
+RP PE+ + R GII A+ Y G+ IG+G++AVV+E H T + A+K+ DK RL DMVD E+ I ++ +HPNI +L+ YET+ E+++++E V+GGDLFD+I+ +++F E A+++I DL AL ++H +++VHRD+KPEN+L+ + ++KL DFGLA ++R VCGTPTY+APEIL EKGYG EVD WA GVILYIL+ GFPPFR+P+R Q EL++ I G +++ +W+NIS A+ L+ LLV P++RYTA V+ W+ T G T
Sbjct: 327 TRPEENKPERPSGRKPRPMGII-----AANVEKHYETGRVIGDGNFAVVKECRHRETRQAYAMKIIDKSRLKGKEDMVDSEILIIQSLSHPNIVKLHEVYETDMEIYLILEYVQGGDLFDAIIESVKFPEPDAALMIMDLCKALVHMHDKSIVHRDLKPENLLVQRNEDKSTTLKLADFGLAKHVVR--PIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQLGHFEFLPPYWDNISDAAKDLVSRLLVVDPKKRYTAHQVLQHPWIETAGKT 619
BLAST of SMED30035230 vs. UniProt/SwissProt
Match: sp|Q8BWQ5|DCLK3_MOUSE (Serine/threonine-protein kinase DCLK3 OS=Mus musculus OX=10090 GN=Dclk3 PE=1 SV=2) HSP 1 Score: 283.493 bits (724), Expect = 2.586e-82 Identity = 138/300 (46.00%), Postives = 200/300 (66.67%), Query Frame = -2
Query: 278 PSRPSTALPEKVFTKNKRRGIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLAT-ILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGT 1174
P RPS P + +GII ++ Y +G IG+G++A V+E H T + A+K+ DK +L D+VD E+ I ++ +HPNI +L+ YETEAE++++ME V+GGDLFD+IV N++F E A+++I DL AL ++H +N+VHRD+KPEN+L+ + I++KL DFGLA ++R VCGTPTY+APEIL EKGYG EVD WA GVILYIL+ GFPPFR+P+R Q EL++ I G +++ +W+NIS A+ L+ +LL P++RYTA V+ W+ +G T
Sbjct: 492 PERPSGRKP-------RPKGII-----SADVEKHYDIGGVIGDGNFATVKECRHRETKQAYAMKMIDKSQLKGKEDIVDSEILIIQSLSHPNIVKLHEVYETEAEIYLIMEYVQGGDLFDAIVENVKFPEPEAAVMITDLCKALVHMHDKNIVHRDVKPENLLVQRNEDKSITLKLADFGLAKYVVR--PIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQVGQFEFLSPYWDNISDAAKDLVRNLLEVDPKKRYTAEQVLQHPWIEMVGHT 777 HSP 2 Score: 62.3882 bits (150), Expect = 1.269e-8 Identity = 44/137 (32.12%), Postives = 70/137 (51.09%), Query Frame = -2
Query: 1781 DKPVKSPRARQINQKVANLEASHLNDEHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYK-ERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKEGLSRKIVKEILEDIYYPNNAEA 2188
+KPV PR + + EHS +++TVV+ G P L+ R + + +L++IS++L +K +RV KLF L G E+ D F+ D FIA+GKE L+ K ++ +E++Y N A A
Sbjct: 64 EKPVLGPRGAVMPLFSPQSSLHSVRAEHSPLK---PRVVTVVKLGGQPLRKATLLLNRRSVQTFEQLLSDISEALGFPRWKNDRVRKLFTLKGREVKSVSDFFREGDAFIAMGKEPLTLKSIQLAMEELYPKNRALA 197
BLAST of SMED30035230 vs. UniProt/SwissProt
Match: sp|Q5MPA9|DCLK2_RAT (Serine/threonine-protein kinase DCLK2 OS=Rattus norvegicus OX=10116 GN=Dclk2 PE=1 SV=2) HSP 1 Score: 273.863 bits (699), Expect = 5.319e-79 Identity = 128/267 (47.94%), Postives = 181/267 (67.79%), Query Frame = -2
Query: 296 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1096
E+ L KYR+GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV I R HPNI L ET E+F+VMELVKGGDLFD+I + +++E S ++++LASAL YLH ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + R TA +++ WV
Sbjct: 401 ESFTLLEKYRIGKVIGDGNFAVVKECMDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETTTELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLASALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV 666
BLAST of SMED30035230 vs. UniProt/SwissProt
Match: sp|D2I3C6|DCLK2_AILME (Serine/threonine-protein kinase DCLK2 OS=Ailuropoda melanoleuca OX=9646 GN=DCLK2 PE=3 SV=1) HSP 1 Score: 273.478 bits (698), Expect = 1.074e-78 Identity = 127/267 (47.57%), Postives = 182/267 (68.16%), Query Frame = -2
Query: 296 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1096
E+S L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV I R HPNI L ET E+F+VMELVKGGDLFD+I + +++E S ++++LA+AL YLH ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + R TA +++ WV
Sbjct: 403 ESSTLLEKYKIGKVIGDGNFAVVKECMDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV 668
BLAST of SMED30035230 vs. UniProt/SwissProt
Match: sp|Q8N568|DCLK2_HUMAN (Serine/threonine-protein kinase DCLK2 OS=Homo sapiens OX=9606 GN=DCLK2 PE=1 SV=4) HSP 1 Score: 271.937 bits (694), Expect = 2.648e-78 Identity = 127/267 (47.57%), Postives = 181/267 (67.79%), Query Frame = -2
Query: 296 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1096
E+S L KY++GK IG+G++AVV+E +TG+E A+K+ DK + ++++EV I R HPNI L ET E+F+VMELVKGGDLFD+I + +++E S ++++LA+AL YLH ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q +L+D I G ++P +W+NI+ A+ LI +L + R TA ++ WV
Sbjct: 386 ESSTLLEKYKIGKVIGDGNFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWV 651
BLAST of SMED30035230 vs. TrEMBL
Match: A0A5J4NKT6 (Doublecortin-like kinase 3 (Fragment) OS=Paragonimus westermani OX=34504 GN=DEA37_0009167 PE=3 SV=1) HSP 1 Score: 296.204 bits (757), Expect = 1.754e-89 Identity = 138/298 (46.31%), Postives = 203/298 (68.12%), Query Frame = -2
Query: 203 VFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTH---NIDSQSRSRRMELDRQL 1087
+ +Y +G+ +G+G++AVVR TG+ A+K+ DK +L +M+ +E+ I CNHPNI RL +ET EV++ ME VK GDLFD I ++++FSE A++I+ DLA+AL+YLH R++VHRD+KPEN+L+ G+I +KL DFGLA +R N + +CGTPTYIAPEIL E+GYG EVD WALG+I YI++ GF PFRTPDR+Q++L++ I +GV+ Y +W+++S A+ L+ LLV PR+R TA + I WV G L D+ +D Q R+ R+ L++++
Sbjct: 3 ILQKRYHIGRILGDGNFAVVRLARRRETGQLYAMKIIDKAKLKGKENMLQNEISIMHQCNHPNIVRLIEEFETPNEVWLAMEFVKDGDLFDGITKSVKFSEPVAAVIVADLANALFYLHCRSIVHRDLKPENVLLWRQPDGKIRVKLADFGLALEIRRN-LYTICGTPTYIAPEILCERGYGLEVDMWALGIITYIMLCGFAPFRTPDRRQSKLFESIKRGVFVYLSPYWDDVSQAAKDLVSQLLVVQPRKRLTALETIIHPWVFA-EGCAMKLEDSQATIQMDQQRRALRLNLEKRI 298
BLAST of SMED30035230 vs. TrEMBL
Match: A0A1I8IN06 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 294.278 bits (752), Expect = 2.789e-89 Identity = 142/292 (48.63%), Postives = 197/292 (67.47%), Query Frame = -2
Query: 200 KYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDSQSRSRRMELDRQLI 1075
+Y +G+ +G+G++AVVR +G E A KV DKK+L MV +EV I R C HPNI RL YET+AE++++MELVKGGDLFD+I + ++F+E AS ++ D+A AL+YLHSR +VHRD+KPEN+L+ +IS+KL DFGLA + VCGTPTY+APEIL E GYG EVD WALGVI YIL+ GFPPFR+ DR+Q+EL+ I G ++Y +W+++S A+ LI+ LLV R TA DV+C +WVL+ GG++ + + + + L QL+
Sbjct: 4 RYDLGRTLGDGNFAVVRACVKRDSGNEYAAKVIDKKKLRGKEHMVLNEVRIMRLCRHPNIVRLVDEYETKAEIYLIMELVKGGDLFDAISQVVKFNEIQASSMVADIAGALHYLHSRRIVHRDLKPENLLVYRSKDNKISLKLGDFGLAMEVE-EAIFTVCGTPTYVAPEILSETGYGLEVDMWALGVIAYILLCGFPPFRSRDRRQSELFQMIKTGTFEYLSPYWDHVSAPAKDLINRLLVVDKAARLTAIDVLCHAWVLSRGGSQRQEQEQQTDSAALKDEQSRLRDQLV 294
BLAST of SMED30035230 vs. TrEMBL
Match: K1S2X4 (Serine/threonine-protein kinase DCLK3 OS=Crassostrea gigas OX=29159 GN=CGI_10028458 PE=4 SV=1) HSP 1 Score: 308.531 bits (789), Expect = 3.848e-89 Identity = 143/302 (47.35%), Postives = 211/302 (69.87%), Query Frame = -2
Query: 173 SKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDSQSRSRRMELDRQLIGSFEEFNRL 1078
+KY +G+ +G+G++A+VR+ A E A+KV DK +L MV++E+EI + C+H NI +LY YET ++++VMELVKGGDLFD+I ++++F E ++ ++ DL +AL+YLHSR++VHRD+KPEN+L+ + G IS+KL DFGLA ++ VCGTPTY+APEIL E GYG EVD WA+GVI YIL+ GFPPFR+PDR QTEL++FI G Y++ +W+NIS A+ LI+HLLV ++RYTA + + W+L L G ++ +D ++ R EL+ Q S++ + +L
Sbjct: 481 NKYDLGRTLGDGNFAIVRQSKLKAGSGEYAMKVIDKPKLRGKEHMVENEIEIMKDCHHHNIVKLYEEYETSDKIYLVMELVKGGDLFDAITQSVKFGEVDSARMVKDLCNALFYLHSRSIVHRDLKPENLLVHRNKDGSISLKLADFGLAMEVK-EPIFTVCGTPTYVAPEILSEIGYGLEVDMWAVGVITYILLCGFPPFRSPDRNQTELFEFIKAGEYEFLSPYWDNISPYAKDLIEHLLVVDKKKRYTAIETLSHPWIL-LNGDMSRPPESSKLDEMRKNTRKELELQAKQSYDSYQKL 780 HSP 2 Score: 69.707 bits (169), Expect = 2.311e-8 Identity = 35/109 (32.11%), Postives = 67/109 (61.47%), Query Frame = -2
Query: 1754 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKEGLSRKIVKEILEDIYYPNNAEARSMLEYWDR 2080
K++T+VR G PR ++ L+ R + ++ ++ +IS++ +V KLF++ G E+ D F+ +DVFI VG E L+ + +I+E++ YP+++ A+S+L +R
Sbjct: 97 KVVTIVRNGHKPRNNVKILLNRRSVQSFEQLMADISEAFGPKYKNNKVKKLFSIRGREVQGISDFFRDDDVFIGVGGESLTETDIVDIVEEL-YPDSSMAKSILRELER 204
BLAST of SMED30035230 vs. TrEMBL
Match: V4A365 (Protein kinase domain-containing protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_194203 PE=4 SV=1) HSP 1 Score: 289.656 bits (740), Expect = 2.669e-88 Identity = 131/261 (50.19%), Postives = 191/261 (73.18%), Query Frame = -2
Query: 284 MGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLG 1066
MG+ +G+G++AVV++ +TG+E IKV DK +L MV++E+EI + CNH NI +LY +ET +++VMELVKGGDLFD+I ++++F+E A+ ++ DL +AL+YLHSRN+VHRD+KPEN+L+ + G +S+KL DFGLA + + + VCGTPTY+APEIL E GY EVD WA+GVI+YIL+ GFPPFR+PDR+QTEL+ I G Y++ + +W+ S+ A+ LI HLLV +RY++ DV+ W++ G
Sbjct: 1 MGRVLGDGNFAVVKQSRLRSTGQEFGIKVIDKSKLKGKEHMVENEIEIMKECNHFNIVKLYEEFETRDRLYLVMELVKGGDLFDAITQSVKFTEIEAARMVKDLCNALFYLHSRNIVHRDLKPENLLVHRNKDGTVSLKLADFGLAMEVN-DLIYTVCGTPTYVAPEILSEIGYSLEVDMWAVGVIMYILLCGFPPFRSPDRKQTELFAMIKAGEYEFVMPYWDGTSLYAKNLIGHLLVVDRTKRYSSIDVLSHPWIIFNG 260
BLAST of SMED30035230 vs. TrEMBL
Match: A0A267FWU6 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022282g3 PE=4 SV=1) HSP 1 Score: 300.056 bits (767), Expect = 3.945e-88 Identity = 149/318 (46.86%), Postives = 206/318 (64.78%), Query Frame = -2
Query: 227 SRPSTALPEKVFTKNKRRGIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNI---DSQSRSR 1171
S P + + +++R +I I +Y +G+ +G+G++AVVR +G E A KV DKK+L MV +EV I R C HPNI RL YET+AE++++MELVKGGDLFD+I + ++F+E AS ++ D+A AL+YLHSR +VHRD+KPEN+L+ +IS+KL DFGLA + VCGTPTY+APEIL E GYG EVD WALGVI YIL+ GFPPFR+ DR+Q+EL+ I G ++Y +W+++S A+ LI+ LLV R TA DV+C +WVL+ GG++ T + D QS R
Sbjct: 230 SEPKESTDKAAAPTDRKRPLIRPISSTRYVFERYDLGRTLGDGNFAVVRACVKRDSGNEYAAKVIDKKKLRGKEHMVLNEVRIMRLCRHPNIVRLVDEYETKAEIYLIMELVKGGDLFDAISQVVKFNEIQASSMVADIAGALHYLHSRRIVHRDLKPENLLVYRSKDNKISLKLGDFGLAMEVE-EAIFTVCGTPTYVAPEILSETGYGLEVDMWALGVIAYILLCGFPPFRSRDRRQSELFQMIKTGTFEYLSPYWDHVSAPAKDLINRLLVVDKAARLTAIDVLCHAWVLSRGGSQRQEQQTDSAALKDEQSSLR 546
BLAST of SMED30035230 vs. Ensembl Cavefish
Match: dclk2a (doublecortin like kinase 2 [Source:NCBI gene;Acc:103023338]) HSP 1 Score: 284.648 bits (727), Expect = 2.562e-84 Identity = 133/276 (48.19%), Postives = 188/276 (68.12%), Query Frame = -2
Query: 266 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNL 1093
+S L KY++GK IG+G++AVV++ +TG+E A+K+ DK + ++++EV + R HPNI L +T +E+++VMELVKGGDLFDSI + +++E AS ++++LA AL YLH ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + QQ +L+D I +G D+P +WENIS A+ LI +L + RYTA DV+C WV N+
Sbjct: 419 SSSILEKYKVGKIIGDGNFAVVKDCVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLMEEVDTPSELYLVMELVKGGDLFDSITSSTKYTERDASAMVYNLAGALKYLHRMSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATLVEG-PLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNQQEDLFDQILQGRLDFPSPYWENISHSAKELIGRMLQVNVEARYTAEDVLCHPWVTEDAAMENNM 693 HSP 2 Score: 55.0694 bits (131), Expect = 2.512e-7 Identity = 30/88 (34.09%), Postives = 49/88 (55.68%), Query Frame = -2
Query: 1847 EHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2110
E + F K++TV+R+G PR +R L+ + + VLT+I+ ++ S V +L+ L G++I +D F +DVFIA G E
Sbjct: 181 ERENKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDS--GVVKRLYTLEGKQITCLQDFFGEDDVFIACGPE 266
BLAST of SMED30035230 vs. Ensembl Cavefish
Match: dclk2a (doublecortin like kinase 2 [Source:NCBI gene;Acc:103023338]) HSP 1 Score: 285.034 bits (728), Expect = 3.613e-84 Identity = 132/266 (49.62%), Postives = 186/266 (69.92%), Query Frame = -2
Query: 296 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1093
+S L KY++GK IG+G++AVV++ +TG+E A+K+ DK + ++++EV + R HPNI L +T +E+++VMELVKGGDLFDSI + +++E AS ++++LA AL YLH ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + QQ +L+D I +G D+P +WENIS A+ LI +L + RYTA DV+C WV
Sbjct: 435 SSSILEKYKVGKIIGDGNFAVVKDCVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLMEEVDTPSELYLVMELVKGGDLFDSITSSTKYTERDASAMVYNLAGALKYLHRMSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATLVEG-PLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNQQEDLFDQILQGRLDFPSPYWENISHSAKELIGRMLQVNVEARYTAEDVLCHPWV 699 HSP 2 Score: 55.4546 bits (132), Expect = 2.437e-7 Identity = 30/88 (34.09%), Postives = 49/88 (55.68%), Query Frame = -2
Query: 1847 EHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2110
E + F K++TV+R+G PR +R L+ + + VLT+I+ ++ S V +L+ L G++I +D F +DVFIA G E
Sbjct: 181 ERENKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDS--GVVKRLYTLEGKQITCLQDFFGEDDVFIACGPE 266
BLAST of SMED30035230 vs. Ensembl Cavefish
Match: dclk2a (doublecortin like kinase 2 [Source:NCBI gene;Acc:103023338]) HSP 1 Score: 283.878 bits (725), Expect = 4.064e-84 Identity = 132/266 (49.62%), Postives = 186/266 (69.92%), Query Frame = -2
Query: 296 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1093
+S L KY++GK IG+G++AVV++ +TG+E A+K+ DK + ++++EV + R HPNI L +T +E+++VMELVKGGDLFDSI + +++E AS ++++LA AL YLH ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + QQ +L+D I +G D+P +WENIS A+ LI +L + RYTA DV+C WV
Sbjct: 371 SSSILEKYKVGKIIGDGNFAVVKDCVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLMEEVDTPSELYLVMELVKGGDLFDSITSSTKYTERDASAMVYNLAGALKYLHRMSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATLVEG-PLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNQQEDLFDQILQGRLDFPSPYWENISHSAKELIGRMLQVNVEARYTAEDVLCHPWV 635 HSP 2 Score: 55.4546 bits (132), Expect = 1.877e-7 Identity = 30/88 (34.09%), Postives = 49/88 (55.68%), Query Frame = -2
Query: 1847 EHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2110
E + F K++TV+R+G PR +R L+ + + VLT+I+ ++ S V +L+ L G++I +D F +DVFIA G E
Sbjct: 182 ERENKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDS--GVVKRLYTLEGKQITCLQDFFGEDDVFIACGPE 267
BLAST of SMED30035230 vs. Ensembl Cavefish
Match: dclk2a (doublecortin like kinase 2 [Source:NCBI gene;Acc:103023338]) HSP 1 Score: 285.804 bits (730), Expect = 8.636e-84 Identity = 133/276 (48.19%), Postives = 188/276 (68.12%), Query Frame = -2
Query: 266 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNL 1093
+S L KY++GK IG+G++AVV++ +TG+E A+K+ DK + ++++EV + R HPNI L +T +E+++VMELVKGGDLFDSI + +++E AS ++++LA AL YLH ++VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + QQ +L+D I +G D+P +WENIS A+ LI +L + RYTA DV+C WV N+
Sbjct: 435 SSSILEKYKVGKIIGDGNFAVVKDCVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLMEEVDTPSELYLVMELVKGGDLFDSITSSTKYTERDASAMVYNLAGALKYLHRMSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATLVEG-PLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNQQEDLFDQILQGRLDFPSPYWENISHSAKELIGRMLQVNVEARYTAEDVLCHPWVTEDAAMENNM 709 HSP 2 Score: 55.0694 bits (131), Expect = 3.265e-7 Identity = 30/88 (34.09%), Postives = 49/88 (55.68%), Query Frame = -2
Query: 1847 EHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2110
E + F K++TV+R+G PR +R L+ + + VLT+I+ ++ S V +L+ L G++I +D F +DVFIA G E
Sbjct: 181 ERENKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDS--GVVKRLYTLEGKQITCLQDFFGEDDVFIACGPE 266
BLAST of SMED30035230 vs. Ensembl Cavefish
Match: dclk1b (doublecortin-like kinase 1b [Source:ZFIN;Acc:ZDB-GENE-080516-7]) HSP 1 Score: 270.011 bits (689), Expect = 5.542e-79 Identity = 129/260 (49.62%), Postives = 177/260 (68.08%), Query Frame = -2
Query: 296 KYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1075
+YR+G+ IG+G++AVVRE +TGRE A+K+ +K + M+ +EV I R HPN+ L +T +E+++VMELVKGGDLFD+I +++E AS ++++LASA+ YLHS N+VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR Q L+D I G D+PL +W+N+S A+ LI +L +RYTA V+ WV
Sbjct: 366 RYRVGRMIGDGNFAVVRECVERSTGREYALKIINKGKCRGKEHMIQNEVSILRRVKHPNVVLLIEEMDTYSELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATVVDG-PLYTVCGTPTYVAPEIITESGYGLKVDIWAAGVITYILLCGFPPFRGSTEDQEALFDQILMGQLDFPLPYWDNVSDSAKELITCMLQVEVNQRYTALQVLDHPWV 624 HSP 2 Score: 55.0694 bits (131), Expect = 3.149e-7 Identity = 35/118 (29.66%), Postives = 63/118 (53.39%), Query Frame = -2
Query: 1847 PSFSDKPVKSPRARQINQKVANLEASHLNDEHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2200
P++S K S RA +A+ +A L+ + S D F K++T++R+G PR +R L+ + VL++I+ ++ + V +L+ + G+ I +D F +D+FIA G E
Sbjct: 142 PNWSVKTTASSRA---PSSLASTKACSLDAKESKD-FIRPKLVTIIRSGVKPRKAVRILLNKKTAHTFEQVLSDITDAIKLDTGA--VKRLYTVDGKLISCLQDFFGEDDIFIACGPE 253
BLAST of SMED30035230 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010548.1 (pep scaffold:Pmarinus_7.0:GL477749:266235:284843:-1 gene:ENSPMAG00000009555.1 transcript:ENSPMAT00000010548.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 245.358 bits (625), Expect = 3.712e-71 Identity = 124/285 (43.51%), Postives = 182/285 (63.86%), Query Frame = -2
Query: 263 SKYRMGKKIGEGHYAVVREGSHLATGR--------EVAIKVYDKKRLTNAMDMVDDEVEIQ-RACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENIS---MEARFLIDHLLVTHPRERYTARDVICDSWVLTLG-GTRGNLH 1078
S+Y +G+ IG+G++A V+E +++G ++A+K+ DK +L +M+ EV + R HPN+ RL A E+ ++ ++V+E V+GGDLFD+I ++RF+E A+ ++ DL AL YLH+ N+VHRD+KPEN+L+ + G ++KL D GLA + G + VCGTPTY+APEIL E GYG EVD WA GVI YIL+ GFPPFR+ +R Q EL++ I G Y++ +W++IS A+ L+ LLV RYTAR + WV G G +LH
Sbjct: 361 SRYEIGRTIGDGNFATVKECRRVSSGDGSAGEAEWDLAMKIIDKAKLVGREEMLRSEVALTTRLGQHPNVVRLVEAMESPSDAYLVLERVRGGDLFDAITESVRFTERHAAAMVADLCRALQYLHANNIVHRDVKPENLLVQRNADGGTTLKLADLGLAMEVTG-PIYTVCGTPTYVAPEILAETGYGLEVDMWAAGVITYILLCGFPPFRSLERDQEELFEIIQLGEYEFLSPYWDHISDGHEAAKDLVSRLLVVDTARRYTARQALHHPWVSDGGAGDHTDLH 644
BLAST of SMED30035230 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008748.1 (pep scaffold:Pmarinus_7.0:GL476577:591:63029:-1 gene:ENSPMAG00000007907.1 transcript:ENSPMAT00000008748.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 203.756 bits (517), Expect = 4.952e-59 Identity = 108/302 (35.76%), Postives = 175/302 (57.95%), Query Frame = -2
Query: 191 FLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMD--MVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHL--VCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDSQSRSRRMELDRQLIGSF 1084
F +Y++ +++G+G ++VVR ++TG+E A K+ + K+L+ A D ++ E I R H NI RL+ + E ++V +LV GG+LF+ IV +SE AS I + ++++ H +VHRD+KPEN+L++ + G ++KL DFGLA ++G+Q GTP Y++PE+L + YG VD WA GVILYIL+ G+PPF D Q +LY I G YD+P W+ ++ EA+ LI+ +L +P +R TA + + W+ +H ++ R+ R+L G+
Sbjct: 10 FTDEYQLYEELGKGAFSVVRRCVKVSTGQEFAAKIINTKKLS-ARDHQKLEREARICRLLKHSNIVRLHESIAEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILESVHHCHQHGIVHRDLKPENLLLASKMKG-AAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPYGKPVDMWACGVILYILLVGYPPFW--DEDQHKLYQQIKAGAYDFPSPEWDTVTAEAKNLINQMLTINPAKRITAAEALKHPWICQRSTVASCMHRQETVECL---RKFNARRKLKGAI 304
BLAST of SMED30035230 vs. Ensembl Sea Lamprey
Match: stk33 (serine/threonine kinase 33 [Source:ZFIN;Acc:ZDB-GENE-040724-242]) HSP 1 Score: 186.037 bits (471), Expect = 2.430e-53 Identity = 103/272 (37.87%), Postives = 158/272 (58.09%), Query Frame = -2
Query: 296 FLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRL-TNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILI--SEDIHG--RISIKLTDFGLATILRGNQAHLV----CGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1084
F + YR+G+++G G + V E +H+ TG + AIK +K+R ++ + +++ EV I + H NI L ET V++VMEL + G+L + + R SE A + H LA A+ YLH ++VHRD+K ENIL SE G + I++TDFGL+ + GN A + CGTP Y+A E+++ + Y + D W++GVILY+L+ G PPF Q +L++ I KG + WE +S A+ L+ LL P R TA +++ D WV
Sbjct: 1 FQNYYRLGRQLGSGSFGTVYEATHVETGVKWAIKTVNKERAGSSGVRLLEREVVILKRVQHQNIIHLEEVLETHKRVYLVMELCELGELRAVLEQRKRLSEAEARHVAHSLAGAIAYLHKHDIVHRDLKLENILAKSSEPHEGIDHLDIRVTDFGLSAVREGNGAASMLQSPCGTPIYMAAEVIQSQDYSQQCDVWSIGVILYMLLCGEPPFMA--HSQEKLFELIIKGELSFTQPAWEEVSESAKELLRGLLKVDPAHRLTASELLHDHWV 270
BLAST of SMED30035230 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007114.1 (pep scaffold:Pmarinus_7.0:GL477366:299514:356138:-1 gene:ENSPMAG00000006368.1 transcript:ENSPMAT00000007114.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 196.823 bits (499), Expect = 2.608e-53 Identity = 107/269 (39.78%), Postives = 155/269 (57.62%), Query Frame = -2
Query: 296 SVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGL---ATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPD-RQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1090
S +KY++GK IG+G++AVVRE + + + M++ EV I R HPNI L +T +E++MVMELVKGGDLFD+I + +++E AS ++++L SA+ YLH+ N ++RDIKPEN+L+ +H LTD+ L A L HL T++ P + YG +VD WA GVI YIL+ GFPPFR D Q L+D I G D+P +W+NIS+ A+ LI +L +R++A V+ WV
Sbjct: 408 SAITAKYKIGKVIGDGNFAVVRECIERMMIASYMLNIIQRHPPRPQEHMIESEVSILRRVKHPNIILLVEEVDTPSELYMVMELVKGGDLFDAITSSTKYTERDASGMLYNLTSAVAYLHALN-INRDIKPENLLVRAGMHYSHCTDLTDYSLLIFAATLSPGPLHLSVCDLTFVCP---LPRSYGLKVDIWAAGVIAYILLCGFPPFRASDGDDQEALFDQILVGQLDFPSPYWDNISLTAKELISRMLQVDVDKRFSAEQVLAHPWV 672 HSP 2 Score: 51.6026 bits (122), Expect = 7.052e-7 Identity = 26/78 (33.33%), Postives = 46/78 (58.97%), Query Frame = -2
Query: 1847 KMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2080
K++TV+R+G PR +R L+ + + VLT+I+ ++ + V +L+ L G+++ +D F +DVFIA G E
Sbjct: 192 KLVTVIRSGVKPRKAVRILLNKKTAHSFDQVLTDITDAIKLDT--GVVKRLYTLDGKQVTCLQDFFGDDDVFIACGPE 267
BLAST of SMED30035230 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006410.1 (pep scaffold:Pmarinus_7.0:GL476370:17405:31068:-1 gene:ENSPMAG00000005688.1 transcript:ENSPMAT00000006410.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 191.43 bits (485), Expect = 6.048e-52 Identity = 109/274 (39.78%), Postives = 162/274 (59.12%), Query Frame = -2
Query: 296 GEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLT-NAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLA---TILRGNQAHLVCGTPTYIAPEILEEKGY-GTEVDNWALGVILYILISGFPPFRTPDRQQ-TELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1099
GE + YR+ K IG+G++A V+ H+ TGREVAIK+ DK +L ++ + EV I + NHPNI +LY ETE +++VME GG++FD +V + R E+ A + SA+ Y H +N+VHRD+K EN+L+ D+H IK+ DFG + T++ N+ CG+P Y APE+ + K Y G EVD W+LGVILY L+SG PF D Q EL + + +G Y P F+ +S + L+ LV +P +R + ++ D W+
Sbjct: 30 GEELPHIGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKCLNHPNIVKLYEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKNIVHRDLKAENLLLDVDMH----IKIADFGFSNEFTLV--NKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPF---DGQNLKELREKVLRGKYRIP--FY--MSTDCENLLKRFLVLNPIKRGSLEQIMRDRWM 290
BLAST of SMED30035230 vs. Ensembl Yeast
Match: DUN1 (Cell-cycle checkpoint S/T protein kinase; required for transient G2/M arrest after DNA damage, damage-induced transcription, and nuclear-to-cytoplasmic redistribution of Rnr2p-Rnr4p after genotoxic stress and iron deprivation; phosphorylates repair protein Rad55p, transcriptional repressor Sml1p, superoxide dismutase, and ribonucleotide reductase inhibitors Crt1p and Dif1p; functions in the Mec1p pathway to regulate dNTP pools and telomere length; postreplicative repair role [Source:SGD;Acc:S000002259]) HSP 1 Score: 184.496 bits (467), Expect = 8.596e-51 Identity = 96/285 (33.68%), Postives = 158/285 (55.44%), Query Frame = -2
Query: 299 FLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMD--MVDDEVEIQRACNHPNIARLYAAY-----ETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILIS---------------EDIHGRISIKLTDFGLATILRGNQ-AHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSW 1084
F KY +GK++G GHYA+V+E + TG++VA+K++ ++ + +E I HPNI L ++ +++ + ++V+E + G+LF+ IVR ++ + + L + L YLH +N++HRDIKPENIL++ ++ I +K+ DFGLA Q + +CGTP+Y+APE+L +KGY ++VD W+ GVILY+ + GFPPF + L + I + Y + +W+ I LI +LLV +P ERY + + W
Sbjct: 196 FFDKYLLGKELGAGHYALVKEAKNKKTGQQVAVKIFHAQQNDDQKKNKQFREETNILMRVQHPNIVNLLDSFVEPISKSQIQKYLVLEKIDDGELFERIVRKTCLRQDESKALFKQLLTGLKYLHEQNIIHRDIKPENILLNITRRENPSQVQLGPWDEDEIDIQVKIADFGLAKFTGEMQFTNTLCGTPSYVAPEVLTKKGYTSKVDLWSAGVILYVCLCGFPPF-SDQLGPPSLKEQILQAKYAFYSPYWDKIDDSVLHLISNLLVLNPDERYNIDEALNHPW 479
BLAST of SMED30035230 vs. Ensembl Yeast
Match: CMK1 (Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication [Source:SGD;Acc:S000001910]) HSP 1 Score: 179.874 bits (455), Expect = 8.773e-50 Identity = 97/270 (35.93%), Postives = 164/270 (60.74%), Query Frame = -2
Query: 293 KYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTN---AMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLV--CGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEF----WENISMEARFLIDHLLVTHPRERYTARDVICDSWVL 1075
KY GK +G G + VVR+ + TG +VA+K+ KK L ++ + DE++I + +HPNI +E++ + +++ +L KGG+LFD I++ +F+EE A I+ ++ SA+ Y+HS+N+VHRD+KPEN+L D + + DFG+A L+ ++ L G+ Y+APE+L + G+G D W++GVI Y L+ G+ FR +R Q + D T G +YP++F W+++S +A+ I L P +R TA +++ D W++
Sbjct: 36 KYVFGKTLGAGTFGVVRQAKNTETGEDVAVKILIKKALKGNKVQLEALYDELDILQRLHHPNIVAFKDWFESKDKFYIITQLAKGGELFDRILKKGKFTEEDAVRILVEILSAVKYMHSQNIVHRDLKPENLLYI-DKSDESPLVVADFGIAKRLKSDEELLYKPAGSLGYVAPEVLTQDGHGKPCDIWSIGVITYTLLCGYSAFRA-ERVQ-DFLDECTTG--EYPVKFHRPYWDSVSNKAKQFILKALNLDPSKRPTAAELLEDPWII 300
BLAST of SMED30035230 vs. Ensembl Yeast
Match: CMK2 (Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication [Source:SGD;Acc:S000005376]) HSP 1 Score: 169.088 bits (427), Expect = 5.232e-46 Identity = 95/279 (34.05%), Postives = 157/279 (56.27%), Query Frame = -2
Query: 290 EASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTN---AMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQ--AHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYD-YPLEF----WENISMEARFLIDHLLVTHPRERYTARDVICDSWVLT 1096
E+ V + Y G+ +G G + VVR+ L+T +VAIK+ KK L + M+ +E+ I + +HPNI +E++ + ++V +L GG+LFD I+ +F+E A II + A+ Y+HS+NVVHRD+KPEN+L D + + DFG+A L+G + + G+ Y+APE+L + G+G D W++GVI Y L+ G+ PF + F+ + YP+ F W+NIS++ + I L +P +R TA +++ D W+ +
Sbjct: 39 ESYVNRTNYIFGRTLGAGSFGVVRQARKLSTNEDVAIKILLKKALQGNNVQLQMLYEELSILQKLSHPNIVSFKDWFESKDKFYIVTQLATGGELFDRILSRGKFTEVDAVEIIVQILGAVEYMHSKNVVHRDLKPENVLYV-DKSENSPLVIADFGIAKQLKGEEDLIYKAAGSLGYVAPEVLTQDGHGKPCDIWSIGVITYTLLCGYSPFIA-----ESVEGFMEECTASRYPVTFHMPYWDNISIDVKRFILKALRLNPADRPTATELLDDPWITS 311
BLAST of SMED30035230 vs. Ensembl Yeast
Match: RAD53 (DNA damage response protein kinase; required for cell-cycle arrest, regulation of copper genes in response to DNA damage; phosphorylates nuclear pores to counteract gene gating, preventing aberrant transitions at forks approaching transcribed genes; activates downstream kinase Dun1p; differentially senses mtDNA depletion, mitochondrial ROS; relocalizes to cytosol under hypoxia; human homolog CHEK2 implicated in breast cancer can complement yeast null mutant [Source:SGD;Acc:S000006074]) HSP 1 Score: 172.555 bits (436), Expect = 3.460e-45 Identity = 96/269 (35.69%), Postives = 148/269 (55.02%), Query Frame = -2
Query: 296 IGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHL--VCGTPTYIAPEIL-------------EEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVY-DYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1054
+G+G +A V++ TG+ A+K+ K+++ MD V E+E+ + NHP I RL YE +MVME V GGDL D + + E+ I + +A+ Y+HS + HRD+KP+NILI +D + +K+TDFGLA + +GN + + CGT Y+APE++ E Y + VD W++G ++Y++++G PF Q +LY I +G Y + PL+ + IS EAR ID LL P R TA + W+
Sbjct: 204 VGQGAFATVKKAIERTTGKTFAVKIISKRKVIGNMDGVTRELEVLQKLNHPRIVRLKGFYEDTESYYMVMEFVSGGDLMDFVAAHGAVGEDAGREISRQILTAIKYIHSMGISHRDLKPDNILIEQD--DPVLVKITDFGLAKV-QGNGSFMKTFCGTLAYVAPEVIRGKDTSVSPDEYEERNEYSSLVDMWSMGCLVYVILTGHLPFS--GSTQDQLYKQIGRGSYHEGPLKDFR-ISEEARDFIDSLLQVDPNNRSTAAKALNHPWI 466
BLAST of SMED30035230 vs. Ensembl Yeast
Match: SNF1 (AMP-activated S/T protein kinase; forms a complex with Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; regulates nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth and acts as a non-canonical GEF, activating Arf3p during invasive growth; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ub ligase [Source:SGD;Acc:S000002885]) HSP 1 Score: 166.777 bits (421), Expect = 7.610e-44 Identity = 96/266 (36.09%), Postives = 152/266 (57.14%), Query Frame = -2
Query: 299 LSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDM---VDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILR-GNQAHLVCGTPTYIAPEILEEKGY-GTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSW 1081
+ Y++ K +GEG + V+ H TG++VA+K+ +KK L + DM ++ E+ R HP+I +LY +++ E+ MV+E G +LFD IV+ + SE+ A + SA+ Y H +VHRD+KPEN+L+ E +++K+ DFGL+ I+ GN CG+P Y APE++ K Y G EVD W+ GVILY+++ PF D L+ I+ GVY P + +S A LI +L+ +P R + +++ D W
Sbjct: 52 IGNYQIVKTLGEGSFGKVKLAYHTTTGQKVALKIINKKVLAKS-DMQGRIEREISYLRLLRHPHIIKLYDVIKSKDEIIMVIEYA-GNELFDYIVQRDKMSEQEARRFFQQIISAVEYCHRHKIVHRDLKPENLLLDE----HLNVKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYVMLCRRLPFD--DESIPVLFKNISNGVYTLP----KFLSPGAAGLIKRMLIVNPLNRISIHEIMQDDW 305
BLAST of SMED30035230 vs. Ensembl Nematostella
Match: EDO44488 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVP7]) HSP 1 Score: 274.633 bits (701), Expect = 1.501e-86 Identity = 134/259 (51.74%), Postives = 182/259 (70.27%), Query Frame = -2
Query: 296 YRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1072
YR+GK IG+G++AVVRE H T +E A+K+ +K ++ M+++E+ I R H +I L YET E+F+VMELVKGGDLF++IV +++E AS ++ DLASAL YLH ++VHRDIKPEN+L+ +GR S+KL DFGLA ++ + LVCGTPTY+APEIL+E GYG +VD WA GVI YIL+ GFPPFR+PD Q EL+D I G ++Y FW +IS A+ LIDH+LV +RY+A+ V+ WV
Sbjct: 1 YRIGKVIGDGNFAVVRECKHRKTNKEYALKIINKAKVKGKEHMIENEISILRRVKHNHIVELIEEYETPREIFLVMELVKGGDLFEAIVEATKYTEVHASHMVRDLASALDYLHCNSIVHRDIKPENLLVLNLPNGRKSLKLADFGLAMEVK-SPMFLVCGTPTYVAPEILDESGYGLKVDIWAAGVISYILLCGFPPFRSPD--QDELFDLILAGEFEYLSPFWNDISSSAKDLIDHMLVVDANQRYSAKQVLAHPWV 256
BLAST of SMED30035230 vs. Ensembl Nematostella
Match: EDO42432 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1C2]) HSP 1 Score: 274.248 bits (700), Expect = 3.405e-83 Identity = 122/258 (47.29%), Postives = 175/258 (67.83%), Query Frame = -2
Query: 299 YRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSW 1072
Y G+K+G+G++A+VR+ T +E AIK+ DK+++ M+DDE+ I R C HPNI RL+ Y++ E+++VMEL+KGGDLFD+I +++F+E A D+ AL YLH R +VHRD+KPEN+L+ + G+ +KL DFGLA + VCGTPTY+APEILEE GYG +VD WA GVI YI++ GFPPFR+P+R Q EL+D I G +++ +W+ +S +A+ LI +LV P +R TA V+ W
Sbjct: 246 YNFGQKLGDGNFAIVRQCKDKITQKEFAIKIIDKRKIRGKEKMIDDEIAIMRRCRHPNIVRLFEDYDSATEIYLVMELIKGGDLFDAISSSVKFTEHVAKSYFRDMCKALAYLHKRKIVHRDLKPENLLVHKRSDGQTHLKLADFGLAMEVTA-PIFTVCGTPTYVAPEILEENGYGLKVDMWAAGVITYIMLCGFPPFRSPNRDQDELFDLIQSGEFEFLSPYWDPVSDDAKDLISTMLVVDPTKRATADQVLQHRW 502
BLAST of SMED30035230 vs. Ensembl Nematostella
Match: EDO49898 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF52]) HSP 1 Score: 202.986 bits (515), Expect = 2.884e-57 Identity = 120/335 (35.82%), Postives = 180/335 (53.73%), Query Frame = -2
Query: 185 IGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTN-AMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQA--HLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVL-------------TLGG---------TRGNLH----DTHNIDSQSRSRRMELDRQLIGSFEE 1102
I E F KY + +++G+G ++ VR+ H T E A+K+ D K +T + V+ E I R HPN+ RL+A ++ F+V +LV GG+LF+ IV +SE AS I + ++ + H +VHRD+KPEN+L++ G + +KL DFGLA + G + + GTP Y++PE+L++ YG VD WA GVILYIL+ G+PPF D +Q +LY I G YDYP W+ ++ +A+ LID +L +R TA + + W++ TL G RG +H THN S SR ++ D I + E
Sbjct: 7 ITEGGTFNEKYELKEELGKGAFSTVRKCCHRETKIEYAVKILDTKNMTQRDVHKVEREARICRHLRHPNVVRLHANIMSDGFYFLVFDLVTGGELFEDIVAREYYSEADASHCIQQVLLSVQHCHENGIVHRDLKPENLLLASRERGAM-VKLADFGLAIEVDGERLGWYGFAGTPGYLSPEVLKKDPYGKPVDLWACGVILYILLVGYPPFW--DEEQQKLYSQIKAGTYDYPSPEWDTVTADAKDLIDKMLTVDAPKRITAAEALKHPWIVNRERIASKVHRQQTLDGLKRFNARRKLRGAIHATILATHNFSSGSRHKKKGSDEDSIATIIE 338
BLAST of SMED30035230 vs. Ensembl Nematostella
Match: EDO36253 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ85]) HSP 1 Score: 191.045 bits (484), Expect = 8.422e-55 Identity = 99/262 (37.79%), Postives = 154/262 (58.78%), Query Frame = -2
Query: 296 KYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENIL-ISEDIHGRISIKLTDFGLATI-LRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1075
KY + + +G G ++ V ATG A+K KK L + V++E+ I + H NI L ++ ++ +++VM+LV+GG+LFD IV ++E+ AS ++ + A YLHS +VHRD+KPEN+L S D +I I +DFGL+ I +G+ CGTP Y+APE+L+++ YG VD W++GVI YIL+ G+PPF D L+ I + Y++ +W+ IS A+ I HL+ P+ RYT R + W+
Sbjct: 16 KYDLREVLGTGAFSEVVLAEEKATGSLFAVKCISKKELKGKEESVENEIAILKKVRHKNIIGLKDIFDNKSHLYLVMDLVQGGELFDRIVEKGNYTEQDASALVQQILEAADYLHSLGIVHRDLKPENLLYYSPDEDSKIMI--SDFGLSKIEAQGSFMDTACGTPGYVAPEVLKQQPYGKAVDCWSIGVITYILLCGYPPFY--DDSDANLFAQIMRAEYEFDSPYWDEISESAKDFIRHLMELDPKLRYTCRQALSHPWI 273
BLAST of SMED30035230 vs. Ensembl Nematostella
Match: EDO44594 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RV77]) HSP 1 Score: 189.119 bits (479), Expect = 3.350e-53 Identity = 108/274 (39.42%), Postives = 156/274 (56.93%), Query Frame = -2
Query: 296 FLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVD---DEVEIQRAC-NHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILR-GNQAHLVCGTPTYIAPEILE------EKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1084
F+ KY + +G G ++VR+ TG+ A+K+ DK D++ DEV I + H NI RL +E+ A F+V E+ G+LFD + + SE+ I+ + A+ Y+H NVVHRD+KPENIL+ E+I+ +K++DFG A L+ G +CGTP Y+APE+L+ GY EVD WA GVILY L+ GFPPF R+Q + I G Y++ WE++S +A+ LI LLV HP+ER TA D + W+
Sbjct: 19 FIDKYEPKELLGCGVSSIVRKCYDKLTGKAFAVKIIDKLSDQGGSDIIATTRDEVNILLSLQGHKNIIRLEDVFESTAFFFLVFEIAPKGELFDYLTEKVTLSEKQTRRIMLSIFEAVDYMHYHNVVHRDLKPENILLDEEIN----VKISDFGFAVELKEGETLRELCGTPGYLAPEVLQCSMFPDAPGYTKEVDMWACGVILYTLLCGFPPFW--HRRQVVMLRNIMNGKYNFSSPEWEDVSNDAKNLIKKLLVVHPKERITASDALKHPWL 286
BLAST of SMED30035230 vs. Ensembl Medaka
Match: ENSORLT00000003850.2 (serine/threonine-protein kinase DCLK2 [Source:NCBI gene;Acc:101173659]) HSP 1 Score: 275.789 bits (704), Expect = 5.825e-81 Identity = 128/266 (48.12%), Postives = 184/266 (69.17%), Query Frame = -2
Query: 296 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1093
+S+ KY++GK IG+G++AVV+E ATG+E A+K+ DK R + ++++EV + R HP+I +L ET +++F+VMELVKGGDLFD+I + ++SE AS ++++LA A+ YLH N+VHRDIKPEN+L+ E G S+KL DFGLAT++ G VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q EL+D I +G ++P W+ IS+ A+ LI +L + R+TA +V+ WV
Sbjct: 421 SSIINEKYKVGKVIGDGNFAVVKECVERATGQEFALKIIDKARCSGKEHLIENEVAVLRRVRHPSIIQLIEVDETPSQLFLVMELVKGGDLFDAITSSTKYSERDASAMVYNLAGAVKYLHRMNIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLFTVCGTPTYVAPEIIAETGYGLKVDIWAAGVIAYILLCGFPPFRSENNVQEELFDQILRGKLEFPSPDWDTISLPAKMLISQMLQVNVDARFTAEEVLSHPWV 685 HSP 2 Score: 51.9878 bits (123), Expect = 2.537e-6 Identity = 29/99 (29.29%), Postives = 52/99 (52.53%), Query Frame = -2
Query: 1847 VANLEASHLNDEHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2143
A+ E S + F K++TV+R+G PR +R L+ + + VL +I++++ S V +L+ L G+++ +D F +DVF+A G E
Sbjct: 198 TASKERSDSRESRESKDFIKPKLVTVIRSGVKPRKAVRVLLNKKTAHSFDQVLADITEAIKLDSGA--VKRLYTLDGKQLTCLQDFFGDDDVFMACGPE 294
BLAST of SMED30035230 vs. Ensembl Medaka
Match: ENSORLT00000031333.1 (serine/threonine-protein kinase DCLK2 [Source:NCBI gene;Acc:101173659]) HSP 1 Score: 275.789 bits (704), Expect = 5.825e-81 Identity = 128/266 (48.12%), Postives = 184/266 (69.17%), Query Frame = -2
Query: 296 ASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1093
+S+ KY++GK IG+G++AVV+E ATG+E A+K+ DK R + ++++EV + R HP+I +L ET +++F+VMELVKGGDLFD+I + ++SE AS ++++LA A+ YLH N+VHRDIKPEN+L+ E G S+KL DFGLAT++ G VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR+ + Q EL+D I +G ++P W+ IS+ A+ LI +L + R+TA +V+ WV
Sbjct: 421 SSIINEKYKVGKVIGDGNFAVVKECVERATGQEFALKIIDKARCSGKEHLIENEVAVLRRVRHPSIIQLIEVDETPSQLFLVMELVKGGDLFDAITSSTKYSERDASAMVYNLAGAVKYLHRMNIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG-PLFTVCGTPTYVAPEIIAETGYGLKVDIWAAGVIAYILLCGFPPFRSENNVQEELFDQILRGKLEFPSPDWDTISLPAKMLISQMLQVNVDARFTAEEVLSHPWV 685 HSP 2 Score: 51.9878 bits (123), Expect = 2.537e-6 Identity = 29/99 (29.29%), Postives = 52/99 (52.53%), Query Frame = -2
Query: 1847 VANLEASHLNDEHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2143
A+ E S + F K++TV+R+G PR +R L+ + + VL +I++++ S V +L+ L G+++ +D F +DVF+A G E
Sbjct: 198 TASKERSDSRESRESKDFIKPKLVTVIRSGVKPRKAVRVLLNKKTAHSFDQVLADITEAIKLDSGA--VKRLYTLDGKQLTCLQDFFGDDDVFMACGPE 294
BLAST of SMED30035230 vs. Ensembl Medaka
Match: dclk1a (serine/threonine-protein kinase DCLK1 [Source:NCBI gene;Acc:101167140]) HSP 1 Score: 268.085 bits (684), Expect = 3.687e-78 Identity = 130/264 (49.24%), Postives = 177/264 (67.05%), Query Frame = -2
Query: 284 KYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLG 1075
+Y++G+ IG+G++AVVRE L+TGRE A+K+ +K + M+ +EV I R HPNI L +T E+++VMELVKGGDLFD+I R++E AS ++H+LA+A+ YLHS N+VHRDIKPEN+L+ E G S+KL DFGLAT++ G + VCGTPTY+APEI+ E GYG +VD WA GVI YIL+ GFPPFR Q L+D I G ++P +W+N+S A+ LI +L +RYTA V+ WV G
Sbjct: 381 RYKVGRMIGDGNFAVVRECVELSTGREYALKIINKGKCRGKEHMIQNEVAILRRVKHPNIVLLIEEVDTYNELYLVMELVKGGDLFDAITSANRYTERDASGMLHNLANAIKYLHSLNIVHRDIKPENLLVYEHADGSKSLKLGDFGLATVVDG-PLYTVCGTPTYVAPEIISETGYGLKVDIWAAGVITYILLCGFPPFRGCSDDQEALFDQILMGQLEFPFPYWDNVSETAKELIRSMLEVEVDQRYTALQVLEHPWVTNEG 643 HSP 2 Score: 51.9878 bits (123), Expect = 2.244e-6 Identity = 29/89 (32.58%), Postives = 48/89 (53.93%), Query Frame = -2
Query: 1847 DEHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE 2113
D + F K++TVVR+G PR +R L+ + + VLT+I+ ++ S V K++ L G+ + +D F ED+F+A G E
Sbjct: 169 DGRENKDFIRPKLVTVVRSGVKPRKAVRILLNKKTAHSYEQVLTDITDAIKLDS--GVVKKIYTLEGKLVSCLQDFFGDEDIFVACGPE 255
BLAST of SMED30035230 vs. Ensembl Medaka
Match: dclk1b (serine/threonine-protein kinase DCLK1 [Source:NCBI gene;Acc:101155370]) HSP 1 Score: 258.84 bits (660), Expect = 2.015e-75 Identity = 124/260 (47.69%), Postives = 178/260 (68.46%), Query Frame = -2
Query: 296 KYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1075
+Y++G+ +G+G++AVVRE +TGRE A+K+ K++ M+ EV I R HPNI L ++ +E+++VMELVKGGDLF +I + +++E AS ++ +LA+A+ YLHS N+VHRDIKPEN+L+ E G S+KL DFGLATI+ G + VCGTPTY+APEI+ E GYG +VD WA G+I YIL+ GFPPFR+ D Q+ L++ I K +D+P +W+N+S A+ LI +L +RYTA V+ WV
Sbjct: 349 RYKVGRTLGDGNFAVVRECVERSTGREYALKIISKEKCKGKEHMIQSEVAILRRVKHPNIVLLVEEIDSPSELYLVMELVKGGDLFYAITSSNKYTERDASCMLFNLANAIKYLHSLNIVHRDIKPENLLVFEHQDGSKSLKLGDFGLATIVNG-PLYAVCGTPTYVAPEIVAETGYGLKVDVWAAGIITYILLCGFPPFRSEDDQEA-LFEQILKCQFDFPTPYWDNVSDAAKTLITKMLEVSVDQRYTAAQVLDHPWV 606
BLAST of SMED30035230 vs. Ensembl Medaka
Match: dclk1b (serine/threonine-protein kinase DCLK1 [Source:NCBI gene;Acc:101155370]) HSP 1 Score: 259.996 bits (663), Expect = 3.521e-75 Identity = 123/260 (47.31%), Postives = 177/260 (68.08%), Query Frame = -2
Query: 296 KYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWV 1075
+Y++G+ +G+G++AVVRE +TGRE A+K+ K++ M+ EV I R HPNI L ++ +E+++VMELVKGGDLF +I + +++E AS ++ +LA+A+ YLHS N+VHRDIKPEN+L+ E G S+KL DFGLATI+ G + VCGTPTY+APEI+ E GYG +VD WA G+I YIL+ GFPPFR+ + Q L++ I K +D+P +W+N+S A+ LI +L +RYTA V+ WV
Sbjct: 385 RYKVGRTLGDGNFAVVRECVERSTGREYALKIISKEKCKGKEHMIQSEVAILRRVKHPNIVLLVEEIDSPSELYLVMELVKGGDLFYAITSSNKYTERDASCMLFNLANAIKYLHSLNIVHRDIKPENLLVFEHQDGSKSLKLGDFGLATIVNG-PLYAVCGTPTYVAPEIVAETGYGLKVDVWAAGIITYILLCGFPPFRSSEDDQEALFEQILKCQFDFPTPYWDNVSDAAKTLITKMLEVSVDQRYTAAQVLDHPWV 643
BLAST of SMED30035230 vs. Planmine SMEST
Match: SMESG000060309.1 (SMESG000060309.1) HSP 1 Score: 1343.56 bits (3476), Expect = 0.000e+0 Identity = 730/730 (100.00%), Postives = 730/730 (100.00%), Query Frame = -2
Query: 170 MPRRMTDFPSNYNSPQIYRRYIWNNGLGNRSKDDVDALPPISNQYGSGYYTPQPSFSDKPVKSPRARQINQKVANLEASHLNDEHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKEGLSRKIVKEILEDIYYPNNAEARSMLEYWDRVVGXXXXXXXXXXXSIEKHENLYNDYKDEGTGHATHNVVTXXXXXXXXXXXGSVASKVLRRHRRRCIECKDDISHFNDKEKANHVELERLRASLIERERARQFXXXXXXXXXXXXXXXXXXXXXXXXXXXXQYELERQMRENLFTSRRAQDERYEVEALRKLSARKRQAPVXXXXXXXXXXXXXXXQTVTLRVPSRPSTALPEKVFTKNKRRGIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDSQSRSRRMELDRQLIGSFEEFNRLI 2359
MPRRMTDFPSNYNSPQIYRRYIWNNGLGNRSKDDVDALPPISNQYGSGYYTPQPSFSDKPVKSPRARQINQKVANLEASHLNDEHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKEGLSRKIVKEILEDIYYPNNAEARSMLEYWDRVVGQKTSRSQKKSKSIEKHENLYNDYKDEGTGHATHNVVTYPYPGYPDYRYGSVASKVLRRHRRRCIECKDDISHFNDKEKANHVELERLRASLIERERARQFREENQSLQERLRLERDRKEREKIIKVREQYELERQMRENLFTSRRAQDERYEVEALRKLSARKRQAPVIIQEIIQREPEPPEIQTVTLRVPSRPSTALPEKVFTKNKRRGIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDSQSRSRRMELDRQLIGSFEEFNRLI
Sbjct: 1 MPRRMTDFPSNYNSPQIYRRYIWNNGLGNRSKDDVDALPPISNQYGSGYYTPQPSFSDKPVKSPRARQINQKVANLEASHLNDEHSHDSFSGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKEGLSRKIVKEILEDIYYPNNAEARSMLEYWDRVVGQKTSRSQKKSKSIEKHENLYNDYKDEGTGHATHNVVTYPYPGYPDYRYGSVASKVLRRHRRRCIECKDDISHFNDKEKANHVELERLRASLIERERARQFREENQSLQERLRLERDRKEREKIIKVREQYELERQMRENLFTSRRAQDERYEVEALRKLSARKRQAPVIIQEIIQREPEPPEIQTVTLRVPSRPSTALPEKVFTKNKRRGIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGTRGNLHDTHNIDSQSRSRRMELDRQLIGSFEEFNRLI 730
BLAST of SMED30035230 vs. Planmine SMEST
Match: SMESG000072629.1 (SMESG000072629.1) HSP 1 Score: 295.434 bits (755), Expect = 1.252e-86 Identity = 128/269 (47.58%), Postives = 193/269 (71.75%), Query Frame = -2
Query: 278 FLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGT 1084
F Y++G IG G+++ V+E ATG+ A K+ DKKRL + D++++E+ I ++C HPNI +L +ET +VF+V E +K GDLFD+I NI+F E +++++ D+ AL Y+HS+ +VHRDIKPEN+L S +G I++KL+DFGLA ++ +++CGTPTY+APEIL E+GYG EVD WA GVI YIL+ GFPPFR+P+R QTEL++FI G ++Y +W+ IS E++ LI+++L+ P +R+T D +C W++TLG +
Sbjct: 652 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIK-NGLINLKLSDFGLAVEIK-EPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNS 918 HSP 2 Score: 51.2174 bits (121), Expect = 3.805e-6 Identity = 24/101 (23.76%), Postives = 61/101 (60.40%), Query Frame = -2
Query: 1793 SGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE--GLSRKIVKEILEDIYYPN 2089
S +++T++R G P + + L+ + + ++ D+L++IS + + + + +++N+ G+EI D F++ F+A+G + L+++ V+ I+ ++ +PN
Sbjct: 102 SKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAEL-FPN 201
BLAST of SMED30035230 vs. Planmine SMEST
Match: SMESG000072629.1 (SMESG000072629.1) HSP 1 Score: 294.664 bits (753), Expect = 9.290e-86 Identity = 128/269 (47.58%), Postives = 193/269 (71.75%), Query Frame = -2
Query: 278 FLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGT 1084
F Y++G IG G+++ V+E ATG+ A K+ DKKRL + D++++E+ I ++C HPNI +L +ET +VF+V E +K GDLFD+I NI+F E +++++ D+ AL Y+HS+ +VHRDIKPEN+L S +G I++KL+DFGLA ++ +++CGTPTY+APEIL E+GYG EVD WA GVI YIL+ GFPPFR+P+R QTEL++FI G ++Y +W+ IS E++ LI+++L+ P +R+T D +C W++TLG +
Sbjct: 750 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIK-NGLINLKLSDFGLAVEIK-EPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNS 1016 HSP 2 Score: 51.6026 bits (122), Expect = 2.749e-6 Identity = 24/101 (23.76%), Postives = 61/101 (60.40%), Query Frame = -2
Query: 1793 SGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE--GLSRKIVKEILEDIYYPN 2089
S +++T++R G P + + L+ + + ++ D+L++IS + + + + +++N+ G+EI D F++ F+A+G + L+++ V+ I+ ++ +PN
Sbjct: 200 SKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAEL-FPN 299
BLAST of SMED30035230 vs. Planmine SMEST
Match: SMESG000078466.1 (SMESG000078466.1) HSP 1 Score: 256.529 bits (654), Expect = 1.512e-72 Identity = 118/259 (45.56%), Postives = 171/259 (66.02%), Query Frame = -2
Query: 413 VTLRVPSRPSTALPEKVFTKNKRRGIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDY 1189
+ LR PS P + + K + + + + ++ + K +G+G++AVV+ H T E A+K+ DK R+ M+++E+ I + CNH NI +L YET EVF+VMELVK GDLFD+I +N++FSE A+ ++ D+A+AL+YLHSR +VHRD+KPEN+++ G I++KL DFGLA ++ + VCGTPTY+APEIL EKGY EVD WALGVI YIL+ GFPPFR+ +R Q EL+ I G +++
Sbjct: 716 ILLRKPSLPGSIVMGK---------LCRQLSSSDIIYQRFEVTKMLGDGNFAVVKLALHRKTKTEYALKIIDKSRMMGKESMIENEIWIMKLCNHDNIVKLKEEYETREEVFLVMELVKNGDLFDAITQNVKFSEPVAAKMVADMANALFYLHSRRIVHRDLKPENLMVLRHKDGSITLKLADFGLAMEIK-KLIYTVCGTPTYVAPEILSEKGYSLEVDLWALGVITYILLCGFPPFRSSNRNQEELFQIIQSGNFEF 964 HSP 2 Score: 66.2402 bits (160), Expect = 7.303e-11 Identity = 45/140 (32.14%), Postives = 77/140 (55.00%), Query Frame = -2
Query: 1760 RARQINQKVANLEASHLNDEHSHDSFSGSKMITVVRAGP-PPRAIIRALIKRANHKNMADVLTEISQSLVQSSYK--ERVTKLFNLLGEEILDAKDLFKAEDVFIAVG-KEGLSRKIVKEILEDIYYPNNAEARSMLEYW 2167
+++ IN K N LN + K+ITVVR P + +R L+ + + ++ +L++I SL + + + ++FNL G+EI D F+ +D FIAVG E L K +K+IL+D+ YP++ + +L+YW
Sbjct: 113 KSKPINSKNVNYYQDILNQQ------VKPKVITVVRNSPEKSKGFVRILLNKRSVQSYETLLSDIETSLRDHLHHKMQGIGRIFNLFGKEIKSVGDFFRNDDFFIAVGDNETLGVKQIKDILDDL-YPDHPQTSHVLKYW 245
BLAST of SMED30035230 vs. Planmine SMEST
Match: SMESG000078466.1 (SMESG000078466.1) HSP 1 Score: 255.758 bits (652), Expect = 3.884e-72 Identity = 118/259 (45.56%), Postives = 171/259 (66.02%), Query Frame = -2
Query: 413 VTLRVPSRPSTALPEKVFTKNKRRGIIEWIGEASVFLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDY 1189
+ LR PS P + + K + + + + ++ + K +G+G++AVV+ H T E A+K+ DK R+ M+++E+ I + CNH NI +L YET EVF+VMELVK GDLFD+I +N++FSE A+ ++ D+A+AL+YLHSR +VHRD+KPEN+++ G I++KL DFGLA ++ + VCGTPTY+APEIL EKGY EVD WALGVI YIL+ GFPPFR+ +R Q EL+ I G +++
Sbjct: 742 ILLRKPSLPGSIVMGK---------LCRQLSSSDIIYQRFEVTKMLGDGNFAVVKLALHRKTKTEYALKIIDKSRMMGKESMIENEIWIMKLCNHDNIVKLKEEYETREEVFLVMELVKNGDLFDAITQNVKFSEPVAAKMVADMANALFYLHSRRIVHRDLKPENLMVLRHKDGSITLKLADFGLAMEIK-KLIYTVCGTPTYVAPEILSEKGYSLEVDLWALGVITYILLCGFPPFRSSNRNQEELFQIIQSGNFEF 990 HSP 2 Score: 66.6254 bits (161), Expect = 7.249e-11 Identity = 45/140 (32.14%), Postives = 77/140 (55.00%), Query Frame = -2
Query: 1760 RARQINQKVANLEASHLNDEHSHDSFSGSKMITVVRAGP-PPRAIIRALIKRANHKNMADVLTEISQSLVQSSYK--ERVTKLFNLLGEEILDAKDLFKAEDVFIAVG-KEGLSRKIVKEILEDIYYPNNAEARSMLEYW 2167
+++ IN K N LN + K+ITVVR P + +R L+ + + ++ +L++I SL + + + ++FNL G+EI D F+ +D FIAVG E L K +K+IL+D+ YP++ + +L+YW
Sbjct: 139 KSKPINSKNVNYYQDILNQQ------VKPKVITVVRNSPEKSKGFVRILLNKRSVQSYETLLSDIETSLRDHLHHKMQGIGRIFNLFGKEIKSVGDFFRNDDFFIAVGDNETLGVKQIKDILDDL-YPDHPQTSHVLKYW 271 The following BLAST results are available for this feature:
BLAST of SMED30035230 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMED30035230 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of SMED30035230 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of SMED30035230 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of SMED30035230 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of SMED30035230 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of SMED30035230 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30035230 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30035230 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMED30035230 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of SMED30035230 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of SMED30035230 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of SMED30035230 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SMED30035230 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035230 ID=SMED30035230|Name=SMED30035230|organism=Schmidtea mediterranea sexual|type=transcript|length=2429bpback to top protein sequence of SMED30035230-orf-1 >SMED30035230-orf-1 ID=SMED30035230-orf-1|Name=SMED30035230-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=742bp MPRRMTDFPSNYNSPQIYRRYIWNNGLGNRSKDDVDALPPISNQYGSGYYback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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