Dimer_Tnp_hAT domain-containing protein

Overview
NameDimer_Tnp_hAT domain-containing protein
Smed IDSMED30035190
Length (bp)1858
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Dimer_Tnp_hAT domain-containing protein (SMED30035190) t-SNE clustered cells

Violin plots show distribution of expression levels for Dimer_Tnp_hAT domain-containing protein (SMED30035190) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dimer_Tnp_hAT domain-containing protein (SMED30035190) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Dimer_Tnp_hAT domain-containing protein (SMED30035190) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035190

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30035190h1SMcG0003610 Contig39361newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30035190h1SMcG0003610 Contig39361uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A0J7KNG8 (Dimer_Tnp_hAT domain-containing protein OS=Lasius niger OX=67767 GN=RF55_8155 PE=4 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 9.537e-39
Identity = 110/388 (28.35%), Postives = 183/388 (47.16%), Query Frame = 1 
Query:  415 IDEISDSENADGDEDENQFNSFACNHNLXXXXXXXXTEYTYSLDDANINEILQNFRKTVRFFRKSPVRND-ILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEEIGPDDLYNEKHFNILANLIEILKPTEL-VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQS-----GNFPQPELMELPYSPKAPTLSLATMLAARLFGEPNPPFAXXXXXXXXXXPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFGVTKQK--SKTVQNL---------------------FNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLDALIFLKYYF 1488
            ID    SE +D D+D+N+  SF  +   +                +N  +++   RK V+ FR+SP +ND ILQ+Y++++  K+L+LILDC+TRWNSL+ M+ER  ++++CI  AL ++  +  + E  +  + +L   L+P +L VK L +  +TL+TAE  + F+  ++   +T L  K   +L+ RI +RR  +L     +LQ+      +   P+   L          +  +L   L  E        +         AS S    +  +Q  S  L   +E       E+  QKE     V + K  SK ++N+                     +  L +I PTS ++ER FS AG     LR+S+  E +++L FL+ YF
Sbjct:  193 IDNADQSELSDVDDDDNE-GSFIVDCIPSSVKIA-----------SNYKDLITKVRKVVKIFRRSPTKNDLILQKYIQEEHDKRLELILDCKTRWNSLVNMLERFYIVRLCISKALIDLRSEIRFTETEWFAINDLKNSLQPVKLGVKVLCRRDATLITAETTVRFILEKLDNQSTPLSTKLAAALRRRIKQRR-IDLTGTLLYLQNPIKFENDLKNPDDTFLLPKKTIIRKEIKKLLERVLSNEHGEKILCASNT-------ASCSNNSDEDQEQDESRTLSLKEE------LEMKLQKEYPEVKVIESKVLSKNLENVLKKEMIAFENDGTKDRYLRMAYEYLLTIPPTSVEAERAFSAAGYIRNNLRTSLGDESINSLCFLRSYF 554

HSP 2 Score: 56.9954 bits (136), Expect = 9.537e-39
Identity = 34/88 (38.64%), Postives = 49/88 (55.68%), Query Frame = 3 
Query:  138 KMYLNVTLHSQEDSYKLGLIAIEGP*DAYQIHSLVEQKLMDYGIKLR-NILASTQDGASVMNKYAQLNKIENQFCINHAIHLAVVETL 398
            + YLN+ LHS +    LGLI I+G   A    +LVE+ L  + I ++ +I+A T DG +VM K   L     Q C  H I LA+++ L
Sbjct:   85 RRYLNINLHSDKQHRNLGLIRIQGSSSAEHCVNLVEEHLKIFNINIKDDIVAITADGPNVMIKVGNLMPCFQQRCFAHGIQLAIIDIL 172

HSP 3 Score: 28.4906 bits (62), Expect = 9.537e-39
Identity = 14/43 (32.56%), Postives = 23/43 (53.49%), Query Frame = 2 
Query:    2 SESTI*KLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTS 130
            S +TI  +++ F+   K    ++  K+      FS+T DEWTS
Sbjct:   39 SSNTIRSIVIQFSESVKANMINEIQKLQMQNQKFSLTFDEWTS 81
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A2S2QKB9 (Dimer_Tnp_hAT domain-containing protein OS=Sipha flava OX=143950 GN=g.106181 PE=4 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 1.123e-34
Identity = 101/322 (31.37%), Postives = 173/322 (53.73%), Query Frame = 1 
Query:  544 DDANINEILQNFRKTVRFFRKSPVRND-ILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEEIGPDDLYNEKHFNILANLIEILKPTEL-VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQS--GNFPQPELMELPYS-PKAPTLSLATM-LAARLFGEPNPPFAXXXXXXXXXXPIASGSATLVQRLQQSISAVLQD-SKECANDGNFELSFQKELKMFGVTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLDALIFLKYYF 1488
            +D NI+ +++  RK V + ++SP +ND ILQ+YV+ + GK++KL+LDC+ RWNSL+ M+ER +LL+ CI+ +L ++       +  +N++  + E+L P +L V+ + +  +TL T +    FLF ++ + N+ L  +    L  RI +RR  EL  +  +LQ+   ++ Q   ++  +S PK   +    + L  R+  E  P       E+ + E I     TL ++L+  IS   +    +   + +     +KE+ +F     +   ++  F  L SI PTS +SER FS A     KLRS +    LDAL+ L+ YF
Sbjct:   46 NDYNISVVIKKIRKVVMYLKRSPTKNDTILQKYVKAEYGKEIKLLLDCKMRWNSLLDMLERFILLKTCIQKSLMDLNHPVRLEDSDYNLITEITEVLTPVKLTVEAIGRRDATLCTTDAAFKFLFKELFEKNSILANEMRTCLSNRIKKRRRPELSGVLNYLQNPHDDYDQITCLKDLFSLPKNDFIRKQIIKLIERIHIENYP----IKEEQQQRESIKFTQLTLKEKLELKISKSKRTIDPKIQRETDLVKIIKKEMNLFENGGTRGHHLELAFKYLMSIPPTSIESERAFSAAAYMGNKLRSRLGDGTLDALLLLRSYF 363
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A0V1ANV1 (BED-type domain-containing protein (Fragment) OS=Trichinella spiralis OX=6334 GN=T01_9761 PE=4 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.826e-34
Identity = 106/329 (32.22%), Postives = 165/329 (50.15%), Query Frame = 1 
Query:  547 DANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEEIGP-DDLYNEKHFNILANLIEILKPTEL-VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGNFPQPELME---LPYSPKAPTLSLATMLAARLF--------------GEPNPPFAXXXXXXXXXXPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFGVTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLDALIFL 1476
            DA +  +++  RK V  FR+SPV+ND LQ+  ED  GK L LI+D R RWNS++  + R + ++ C+K AL+ +   D+   E    ++++++  L+P  + V  L +   +L TAE VL FL   +K+  + + KK +N++K RI ERRN  L  L  +L + +           L    K    + A  L  R+F               E + P           + IA    TL++R + S    L DS  C      ++  + E+ +F    Q+ KT+Q L++AL +  PTS ++ R FS AG F TKLR S+K E +D L  L
Sbjct:  274 DAEVGNVVKKVRKIVASFRRSPVKNDCLQKMCEDMLGKNLALIMDSRVRWNSMLTNMRRFLEMKDCVKSALKSLSDVDNFLYETELTLISDIVNALEPVAVCVNALGRKDCSLATAETVLEFLLRNLKEQRSDIAKKLFNAMKNRIEERRNASLCGLLCYLDNPSSYVSSSSSNCILTRPNKRDIKATAKDLFDRMFKIQVEETKDIDDSDSEKSDP--------DISKSIADQLDTLIRRRKYS-KCDLMDS--CHGKDIMKI-IKNEMAVFEAVGQRPKTLQKLYDALITAPPTSCETGRSFSAAGLFVTKLRLSLKDETIDTLCLL 590

HSP 2 Score: 54.299 bits (129), Expect = 4.058e-7
Identity = 32/88 (36.36%), Postives = 51/88 (57.95%), Query Frame = 3 
Query:  138 KMYLNVTLHSQEDSYKLGLIAIEGP*DAYQIHSLVEQKLMDYGIKLR-NILASTQDGASVMNKYAQLNKIENQFCINHAIHLAVVETL 398
            K YL + +H+    Y +G+I I G   A  I  ++ +KL  + + ++ +I+A   D A VM K  +L  IE+Q C N+ IHLAVV+ +
Sbjct:  147 KRYLCLNVHTVNKVYGVGMIRINGSVKAAGIIQIILEKLEQFELDMKTDIVALVTDSAFVMRKIGRLLGIEHQLCYNNGIHLAVVDVI 234

HSP 3 Score: 30.8018 bits (68), Expect = 4.058e-7
Identity = 14/51 (27.45%), Postives = 26/51 (50.98%), Query Frame = 2 
Query:    2 SESTI*KLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCSENVFKC 154
            +E  + ++++ F  E + + K +       G  FS++ DEWTSC    + C
Sbjct:  101 TEKGMKEMVMSFGEEIRSEIKKELKLEKGIGRKFSLSLDEWTSCGNKRYLC 151
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A226DDR7 (Putative AC9 transposase OS=Folsomia candida OX=158441 GN=Fcan01_16136 PE=4 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 6.463e-32
Identity = 95/319 (29.78%), Postives = 161/319 (50.47%), Query Frame = 1 
Query:  565 ILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEEIGPDDLYNEKHFNILANLIEILKPTELVKE-LSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGNFP--QPELMELPYSPKAPTLSLATMLAARLFG------EPNPPFAXXXXXXXXXXPIASGSATLVQRLQQSISAVLQ--DSKECANDGNFELSFQKELKMFGVTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLDALIFLKYYF 1488
            I+   R TV+ FR+SP +N+ILQ YV+ +  K+L LILDCRTRWNSL  M+ER  L++ CIK A  ++     + E+ FN +  ++E LKP     E +    S L+T +  L  + ++++   +++  +  +S+K R+ ERR  EL  + ++L +  +     EL +     +     L  ML     G      E N   A E+          S +AT    L + +   +    +++         + ++E+K+F     +   +  +++ L +++PTS +SER FS++G  C K RS +    LD L +LK YF
Sbjct:  343 IIDKIRSTVKMFRRSPKKNEILQNYVKSEFQKELNLILDCRTRWNSLAAMIERFHLIRTCIKKAFIDLRVATNFEEQEFNYIFQVMEALKPIRAAVEVICNRESNLITTDAALNLVISKLRAQKSSVSLELASSIKFRVMERRT-ELTKVLRYLHNRVYANEDEELADTITRTEVQNF-LKKMLTRTRIGTVVGDEESNDTPANEDFTVQ-----PSAAATFKDELNEIMKKAMTPPTTEKSKKSQTLLTTLRQEMKLFEDGGTRGYNLSLIYDYLLTVKPTSVESERAFSVSGYVCNKFRSRLSDSTLDDLCYLKSYF 654
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A4C1XQA5 (Uncharacterized protein OS=Eumeta variegata OX=151549 GN=EVAR_98391_1 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.923e-30
Identity = 94/319 (29.47%), Postives = 162/319 (50.78%), Query Frame = 1 
Query:  547 DANINEILQNFRKTVRFFRKSPVRND-ILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEEIGPDDLYNEKHFNILANLIEILKPTEL-VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGNFPQPE--LMELPYSPKAPTLSLATM------LAARLFGEPNP---PFAXXXXXXXXXXPIASGSATLVQRLQQSISAVLQDSK---ECANDGNFELSFQKELKMFGVTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFEL 1455
            D NI E+++  RK V +F++SP++N+ +LQ+YV+ + GK+L L+LDC+TRWNSL+ M+ER  LL+ CI+ AL ++       +  F ++  ++++L P +L V+ + +  + L TA+  L FL  Q+   N+AL KK    L  RI +RR+  L  +  +LQ+    +    L EL +    P  + A +      +  RL    +      +E   +       +    TL ++LQ  I   ++      +  N+     S ++E+ +F     +   ++  +  L SI PTS + ER FS A     KLRS +   +
Sbjct:  117 DENIKELIEKVRKVVIYFKRSPLKNETVLQKYVKIEHGKELSLLLDCKTRWNSLLTMLERFYLLKSCIQKALIDLNHPICLEDVEFELVNKIVDVLTPIKLTVEAICRRDANLCTADAALNFLIKQLSSKNSALAKKIKTCLVDRIKQRRSDNLTGVLNYLQNPRKDEENQLLSELEWHNLFPVPTQALLRKQIKSIVKRLNSRDDTEEEERSESLTDVELRPETSQPQLTLKEKLQLEIDKSMKSPDPEIQVQNESGMTNSIRREMSLFENGGVRGYYLETAYKYLMSIPPTSVEPERAFSAAAYVGNKLRSRLGISI 435
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000045953.1 (zinc finger BED domain-containing protein 1-like [Source:NCBI gene;Acc:111189856])

HSP 1 Score: 50.8322 bits (120), Expect = 3.993e-6
Identity = 50/188 (26.60%), Postives = 82/188 (43.62%), Query Frame = 1 
Query:  556 INEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEE----IGPDDLY--NEKHFNILANLIEILKPTELVKELSKHTSTLLTAEGVLTF---------LFTQMKQNNT--ALGKKFYNSLKIRISERRNKELITLYKFLQSGNFPQPELMELPY 1068
            I+ +L   R+ V FF KS     +L+   E  +    KLI D  TRWNS   M+ R +  Q  +  AL E        DL   +++   +L +++E+LKP + +       +TLL++E   T          + T MK + T   + K   +++   I  R +    +L +FL       P    LP+
Sbjct:  287 ISRLLGKVRRVVTFFHKSTTAAAVLKAKQEMLQLPPHKLIQDVATRWNSSYDMLTRYLEQQAAVYSALTEKDVKKNAKDLITLSDQDVTVLEDVVEVLKPMKTI-------TTLLSSEQQPTVSMILPLKHSILTAMKHSGTDSQIVKDVKSAIATNIEGRYSNP--SLQQFLNESTALDPRFKSLPH 465
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000061313.1 (SMESG000061313.1)

HSP 1 Score: 670.618 bits (1729), Expect = 0.000e+0
Identity = 351/372 (94.35%), Postives = 358/372 (96.24%), Query Frame = 1 
Query:  394 LYNKNNVIDEISDSENADGDEDENQFNSFACNHNLXXXXXXXXTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEEIGPDDLYNEKHFNILANLIEILKPTEL-VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGNFPQPELMELPYSPKAPTLSLATMLAARLFGEPNPPFAXXXXXXXXXXPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFGVTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLDALIFLKYYFIKKLNP 1506
             YNKNNVIDEISDSENADGDEDENQFNSFA +HNLNIDNDIDNT+YTYSLDDANIN+ILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPM+ERL LLQVCIKHALEEI  DDLYNEK FNILANLIEILKPTEL VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSG FPQP+LMELPYS KAPTLSL+TMLAARLFGEPNPPFAEENIEESEVEPIASGSATLVQRLQQSISAVL DSKEC NDGNFELSFQKELK+FGVTKQKSKTVQNLFN LASIQPTSTDSERVFSIAGNFCTKL SSMKFELLDA IFLKYYFIKKLNP
Sbjct:  220 FYNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTKYTYSLDDANINQILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPMMERLFLLQVCIKHALEEIRRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGAFPQPDLMELPYSTKAPTLSLSTMLAARLFGEPNPPFAEENIEESEVEPIASGSATLVQRLQQSISAVLHDSKECGNDGNFELSFQKELKIFGVTKQKSKTVQNLFNVLASIQPTSTDSERVFSIAGNFCTKLHSSMKFELLDAFIFLKYYFIKKLNP 591

HSP 2 Score: 163.31 bits (412), Expect = 6.683e-43
Identity = 86/120 (71.67%), Postives = 87/120 (72.50%), Query Frame = 3 
Query:   48 KSYNLRTSLQKWLNLVCVSQ*QRMNGLAVVKMYLNVTLHSQEDSYKLGLIAIEGP*DAYQIHSLVEQKLMDYGIKLRNILASTQDGASVMNKYAQLNKIENQFCINHAIHLAVVETLQQK 407
            KSYNLRTSLQKW+NL                          EDSYKLGLIAIEG  DAYQIHSLVEQKLMDYGIKLRNILASTQDGASVM KYAQLNKI NQFCINHAIHLAVVET   K
Sbjct:  130 KSYNLRTSLQKWVNL--------------------------EDSYKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKLRNILASTQDGASVMKKYAQLNKITNQFCINHAIHLAVVETFYNK 223
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000022344.1 (SMESG000022344.1)

HSP 1 Score: 670.618 bits (1729), Expect = 0.000e+0
Identity = 353/372 (94.89%), Postives = 358/372 (96.24%), Query Frame = 1 
Query:  394 LYNKNNVIDEISDSENADGDEDENQFNSFACNHNLXXXXXXXXTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEEIGPDDLYNEKHFNILANLIEILKPTEL-VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGNFPQPELMELPYSPKAPTLSLATMLAARLFGEPNPPFAXXXXXXXXXXPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFGVTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLDALIFLKYYFIKKLNP 1506
             YNKNNVIDEISDSENADGDEDENQFNSFA +HNLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRY EDKEGKK KLILDCRTRWNSLIPM+ERLVLLQVCIKHALEEIG DDLYNEK FNIL NLIEILKPTE  VKELSKHTSTL+TAE VLTFLFTQMKQNNTALGKKFYNSLKI ISERRNKELITLYKFLQSG FPQP+LM+LPYS KAPTLSLATMLAARLFGEPNP FAEENIEESEVEPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFGVTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLDALIFLKYYFIKKLNP
Sbjct:  224 FYNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYDEDKEGKKFKLILDCRTRWNSLIPMMERLVLLQVCIKHALEEIGCDDLYNEKDFNILENLIEILKPTEFAVKELSKHTSTLITAERVLTFLFTQMKQNNTALGKKFYNSLKIGISERRNKELITLYKFLQSGTFPQPDLMKLPYSTKAPTLSLATMLAARLFGEPNPQFAEENIEESEVEPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFGVTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLDALIFLKYYFIKKLNP 595

HSP 2 Score: 162.155 bits (409), Expect = 0.000e+0
Identity = 81/90 (90.00%), Postives = 81/90 (90.00%), Query Frame = 3 
Query:  138 KMYLNVTLHSQEDSYKLGLIAIEGP*DAYQIHSLVEQKLMDYGIKLRNILASTQDGASVMNKYAQLNKIENQFCINHAIHLAVVETLQQK 407
            K YLNVTLHSQEDSYKLGLIAIEG  DAYQIHSLVEQKLM Y IKLRNILASTQDGASVM KYAQLNKIENQFCINHAIHLAVVET   K
Sbjct:  138 KRYLNVTLHSQEDSYKLGLIAIEGSCDAYQIHSLVEQKLMYYDIKLRNILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNK 227

HSP 3 Score: 87.4261 bits (215), Expect = 0.000e+0
Identity = 42/45 (93.33%), Postives = 42/45 (93.33%), Query Frame = 2 
Query:    2 SESTI*KLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCS 136
            SESTI KLIVDFANEKKLQFKDKF KMA SGMCFSITTDEWTSCS
Sbjct:   92 SESTIKKLIVDFANEKKLQFKDKFIKMANSGMCFSITTDEWTSCS 136
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000065760.1 (SMESG000065760.1)

HSP 1 Score: 482.641 bits (1241), Expect = 0.000e+0
Identity = 265/312 (84.94%), Postives = 270/312 (86.54%), Query Frame = 1 
Query:  394 LYNKNNVIDEISDSENADGDEDENQFNSFACNHNLXXXXXXXXTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEEIGPDDLYNEKHFNILANLIEILKPTEL-VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGNFPQPELMELPYSPKAPTLSLATMLAARLFGEPNPPFAXXXXXXXXXXPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFGVTKQK 1326
             YNKNNVIDEISDSENADGDEDENQFNSFA +HNLNIDNDIDNTEYTYSLDDANINEILQNFRKT RF RKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNS+IPM+ERLVLLQVCIKH LEEIG DDLYNEK F  LA+LIEILKPTEL VKELSKHTSTLL  E                            ISERRNKELITLYKFLQSG FPQP+LMELPYS KAPTLSLATMLAARLFGEPNPPFAEENIEESEVEPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFGVTKQK
Sbjct:  247 FYNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTEYTYSLDDANINEILQNFRKTDRFLRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSIIPMMERLVLLQVCIKHVLEEIGCDDLYNEKDFTNLADLIEILKPTELAVKELSKHTSTLLRKE----------------------------ISERRNKELITLYKFLQSGTFPQPDLMELPYSTKAPTLSLATMLAARLFGEPNPPFAEENIEESEVEPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFGVTKQK 530

HSP 2 Score: 169.474 bits (428), Expect = 0.000e+0
Identity = 83/90 (92.22%), Postives = 83/90 (92.22%), Query Frame = 3 
Query:  138 KMYLNVTLHSQEDSYKLGLIAIEGP*DAYQIHSLVEQKLMDYGIKLRNILASTQDGASVMNKYAQLNKIENQFCINHAIHLAVVETLQQK 407
            K YLNVTLHSQEDSYKLGLIAIEG  DAYQIHSLVEQKLMDYGIKLRNILASTQDGASVM KYAQLNKIENQFCINHAIHLAVVET   K
Sbjct:  161 KRYLNVTLHSQEDSYKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKLRNILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNK 250

HSP 3 Score: 90.8929 bits (224), Expect = 0.000e+0
Identity = 44/45 (97.78%), Postives = 44/45 (97.78%), Query Frame = 2 
Query:    2 SESTI*KLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCS 136
            SESTI KLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCS
Sbjct:  115 SESTIKKLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCS 159
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000026901.1 (SMESG000026901.1)

HSP 1 Score: 473.396 bits (1217), Expect = 0.000e+0
Identity = 253/268 (94.40%), Postives = 258/268 (96.27%), Query Frame = 1 
Query:  394 LYNKNNVIDEISDSENADGDEDENQFNSFACNHNLXXXXXXXXTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEEIGPDDLYNEKHFNILANLIEILKPTEL-VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGNFPQPELMELPYSPKAPTLSLATMLAARLFGEPNPPFAXXXXXXXXXXPIASGS 1194
             +NKNNVIDEISDSENADGDEDENQFNSFA +HNLNIDNDIDNTEYTYSLDDA INEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILD RTRWNSLIPM+ERLVLLQVCIKHALEEIG DDLYNEK FNILANLIEILKPTEL VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGK FYNSLKIRISERRNKELITLYKFLQSG FPQP+LMELPYS KAPTLSLATMLAARLFGEPNPPF+EENIEESEVEPIASGS
Sbjct:  247 FHNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTEYTYSLDDAKINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDSRTRWNSLIPMMERLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKIFYNSLKIRISERRNKELITLYKFLQSGTFPQPDLMELPYSTKAPTLSLATMLAARLFGEPNPPFSEENIEESEVEPIASGS 514

HSP 2 Score: 169.859 bits (429), Expect = 0.000e+0
Identity = 83/90 (92.22%), Postives = 83/90 (92.22%), Query Frame = 3 
Query:  138 KMYLNVTLHSQEDSYKLGLIAIEGP*DAYQIHSLVEQKLMDYGIKLRNILASTQDGASVMNKYAQLNKIENQFCINHAIHLAVVETLQQK 407
            K YLNVTLHSQEDSYKLGLIAIEG  DAYQIHSLVEQKLMDYGIKLRNILASTQDGASVM KYAQLNKIENQFCINHAIHLAVVET   K
Sbjct:  161 KRYLNVTLHSQEDSYKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKLRNILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFHNK 250

HSP 3 Score: 84.7297 bits (208), Expect = 0.000e+0
Identity = 40/45 (88.89%), Postives = 43/45 (95.56%), Query Frame = 2 
Query:    2 SESTI*KLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCS 136
            SESTI KLIVDFANEKK+QFKDKFTKMA+SG+C SITTDEWTSCS
Sbjct:  115 SESTIKKLIVDFANEKKIQFKDKFTKMAESGICLSITTDEWTSCS 159

HSP 4 Score: 39.6614 bits (91), Expect = 0.000e+0
Identity = 17/20 (85.00%), Postives = 18/20 (90.00%), Query Frame = 3 
Query: 1203 STTIAAINFCSLAGFKGMCQ 1262
            S +IA INFCSLAGFKGMCQ
Sbjct:  512 SGSIAEINFCSLAGFKGMCQ 531
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000077975.1 (SMESG000077975.1)

HSP 1 Score: 407.527 bits (1046), Expect = 1.116e-176
Identity = 209/218 (95.87%), Postives = 211/218 (96.79%), Query Frame = 1 
Query:  394 LYNKNNVIDEISDSENADGDEDENQFNSFACNHNLXXXXXXXXTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPMVERLVLLQVCIKHALEEIGPDDLYNEKHFNILANLIEILKPTEL-VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGNFPQ 1044
             YNKNNVIDEISDSENADGDEDENQFNSFA  HNLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNS+IPM+ERLVLLQVCIKHALEEIG DDLYNEK FNILANLIEILKPTEL VKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSG FPQ
Sbjct:  247 FYNKNNVIDEISDSENADGDEDENQFNSFAGYHNLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSIIPMMERLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGTFPQ 464

HSP 2 Score: 164.851 bits (416), Expect = 1.116e-176
Identity = 81/90 (90.00%), Postives = 81/90 (90.00%), Query Frame = 3 
Query:  138 KMYLNVTLHSQEDSYKLGLIAIEGP*DAYQIHSLVEQKLMDYGIKLRNILASTQDGASVMNKYAQLNKIENQFCINHAIHLAVVETLQQK 407
            K YLNVTLHSQEDSYKLGLIAIE   DAYQIHSLVEQKLMDYGIKLRNIL STQDGASVM KYAQLNKIENQFCINHAIHLAVVET   K
Sbjct:  161 KRYLNVTLHSQEDSYKLGLIAIECSCDAYQIHSLVEQKLMDYGIKLRNILTSTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNK 250

HSP 3 Score: 90.5077 bits (223), Expect = 1.116e-176
Identity = 44/45 (97.78%), Postives = 44/45 (97.78%), Query Frame = 2 
Query:    2 SESTI*KLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCS 136
            SESTI KLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCS
Sbjct:  115 SESTIKKLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCS 159
The following BLAST results are available for this feature:
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0J7KNG89.537e-3928.35Dimer_Tnp_hAT domain-containing protein OS=Lasius ... [more]
A0A2S2QKB91.123e-3431.37Dimer_Tnp_hAT domain-containing protein OS=Sipha f... [more]
A0A0V1ANV11.826e-3432.22BED-type domain-containing protein (Fragment) OS=T... [more]
A0A226DDR76.463e-3229.78Putative AC9 transposase OS=Folsomia candida OX=15... [more]
A0A4C1XQA51.923e-3029.47Uncharacterized protein OS=Eumeta variegata OX=151... [more]
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSAMXT00000045953.13.993e-626.60zinc finger BED domain-containing protein 1-like [... [more]
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000061313.16.683e-4394.35SMESG000061313.1[more]
SMESG000022344.10.000e+094.89SMESG000022344.1[more]
SMESG000065760.10.000e+084.94SMESG000065760.1[more]
SMESG000026901.10.000e+094.40SMESG000026901.1[more]
SMESG000077975.11.116e-17695.87SMESG000077975.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035190 ID=SMED30035190|Name=Dimer_Tnp_hAT domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1858bp
TAGCGAAAGTACAATTTAAAAATTAATCGTAGATTTTGCTAATGAGAAAA
AGCTACAATTTAAGGACAAGTTTACAAAAATGGCTAAATCTGGTATGTGT
TTCTCAATAACAACGGATGAATGGACTAGCTGTAGTGAAAATGTATTTAA
ATGTGACATTACATTCTCAGGAGGATAGTTATAAATTAGGCCTTATTGCC
ATAGAGGGTCCATGAGATGCCTACCAGATTCACTCATTAGTTGAACAAAA
ATTGATGGATTATGGTATTAAACTTCGTAATATTTTGGCATCAACCCAAG
ATGGGGCGAGTGTTATGAATAAATACGCACAATTAAATAAAATTGAAAAT
CAATTTTGCATCAACCATGCTATTCATTTGGCTGTTGTAGAAACTTTACA
ACAAAAATAATGTAATTGATGAAATTTCAGATTCTGAAAATGCTGATGGC
GATGAAGATGAAAATCAATTTAATTCTTTTGCCTGCAATCATAATTTAAA
TATAGATAATGATATCGATAATACTGAATACACATACAGTTTAGATGATG
CAAACATTAATGAAATACTGCAAAACTTCCGAAAGACAGTCAGATTTTTT
AGAAAATCGCCAGTAAGAAACGACATATTGCAACGATATGTTGAAGATAA
AGAGGGTAAAAAACTTAAACTTATATTGGACTGTAGAACCAGGTGGAACT
CCCTTATACCTATGGTGGAGAGATTGGTTCTACTTCAAGTTTGCATAAAA
CATGCATTGGAAGAAATAGGGCCTGACGATTTATACAATGAAAAACACTT
TAATATTTTAGCAAATTTAATTGAAATATTAAAGCCGACAGAATTGGTAA
AAGAACTAAGCAAACATACTTCAACCTTACTCACTGCGGAAGGAGTATTG
ACATTTTTATTTACTCAAATGAAACAAAACAACACTGCATTGGGGAAAAA
ATTTTATAATTCTTTGAAAATAAGAATTTCAGAAAGGCGAAATAAAGAAT
TAATAACTTTGTATAAATTCTTACAAAGTGGAAATTTTCCACAACCAGAA
TTGATGGAACTTCCGTATTCGCCTAAGGCGCCGACTTTGTCCCTAGCAAC
GATGTTAGCTGCAAGATTATTTGGTGAGCCTAATCCACCATTTGCTGAAG
AGAATATTGAGGAAAGTGAAGTTGAACCAATAGCATCTGGATCGGCTACT
TTAGTACAACGATTGCAGCAATCAATTTCTGCAGTCTTGCAGGATTCAAA
GGAATGTGCCAATGATGGCAATTTTGAGTTATCCTTCCAAAAAGAGTTGA
AAATGTTTGGTGTTACTAAGCAAAAATCGAAAACGGTCCAAAACTTATTC
AATGCTCTGGCATCGATTCAACCCACATCAACAGACAGTGAGAGAGTCTT
TTCGATAGCCGGTAATTTTTGTACCAAATTACGTTCATCCATGAAATTTG
AGCTTTTGGACGCATTAATTTTTCTAAAATATTATTTTATTAAAAAACTA
AATCCATAATTATAAATTATGTTACTTTTCTTTTTTCTATTTTTGATGTA
TAATTAAATAATGAAGTTATGTCAATTTTCAATTTTTTTTATATAATTCT
TAAATAAAAATAATTATTTAAAAATAATATTGCTTAATTTTGAATATTGT
TTGTATTTTTGTTTATTTTCCTTGATTTACAGCTCCAAATGTATGTTTTA
TTGTATATTTAAAGGAACTTTTTTATATTTAAAAAAACCGGGGGTTTGAA
ATTCTTCGGTTTTTTGGATGCAATAGTTTGGACTAATTTTACACTAAATT
TTAAAAACTTGACCAAAGATTGTTCTTAGATTTTATTAGTAAACTATATT
TTAATTAT
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protein sequence of SMED30035190-orf-1

>SMED30035190-orf-1 ID=SMED30035190-orf-1|Name=SMED30035190-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=266bp
MVERLVLLQVCIKHALEEIGPDDLYNEKHFNILANLIEILKPTELVKELS
KHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITL
YKFLQSGNFPQPELMELPYSPKAPTLSLATMLAARLFGEPNPPFAEENIE
ESEVEPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFG
VTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLD
ALIFLKYYFIKKLNP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR008906HATC_C_dom
IPR012337RNaseH-like_sf
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
GO:0003676nucleic acid binding