Anoctamin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035175 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of Anoctamin vs. Ensembl Human
Match: ANO8 (anoctamin 8 [Source:HGNC Symbol;Acc:HGNC:29329]) HSP 1 Score: 377.867 bits (969), Expect = 2.079e-113 Identity = 184/438 (42.01%), Postives = 280/438 (63.93%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIG--LIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR 1473
+TF +D + W + + +PEL + +HR+ YA ++TA+Y++LLRGA+ L +RK V +EFGGG R F+ +E ++++V E F TSQERQSI+++WL +LRA+ + + L++Q I+P L ++ I Q+FP+H IL L + WV QPL++I YFGVKIA+YFAWLG YT A++ P + G L + Q + D+ + ALFN++W+T +L+ WKR +E YKWGT+DS E + E RP F+G I P+TR + YYP WKR F VSLP+ C++CV L+ FQ+ + + K + PK++LA ++ + +KK+A+ LN MEN+RLE +Y HL+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLI RQF+ N++E++ P + R+ + +G+R
Sbjct: 69 MTFPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTRGFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFPVHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSRDVSCVVFALFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGVRRISPITRAEEFYYPPWKRLLFQLLVSLPLCLACLVCVF--LLMLGCFQL-QELVLSVKGLPRLARFLPKVMLALLVSVSAEGYKKLAIWLNDMENYRLESAYEKHLIIKVVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLITRQFLQNVREVLQPHLYRRLGRGELGLR 503 HSP 2 Score: 165.622 bits (418), Expect = 4.397e-41 Identity = 79/162 (48.77%), Postives = 110/162 (67.90%), Query Frame = 1
Query: 1891 LYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
L QAE E+ + KY+D F+D+ EMF+QFGYV +F+ AFPLA L L+NN++EIRSDAFKL + QRPFG V SIG WQ +E + +A+ VN L+G G L R+ S I +V +EH LL+K+LI+ +IP++P W+ +E +E++RREA K E
Sbjct: 729 LTQAELESCMKKYEDTFQDYQEMFVQFGYVVLFSSAFPLAALCALVNNLIEIRSDAFKLCTGLQRPFGQRVESIGQWQKVMEAMGVLAIVVNCYLIGQCGQLQRLFPWLSPEAAIVSVVVLEHFALLLKYLIHVAIPDIPGWVAEEMAKLEYQRREAFKRHE 890
BLAST of Anoctamin vs. Ensembl Human
Match: ANO8 (anoctamin 8 [Source:HGNC Symbol;Acc:HGNC:29329]) HSP 1 Score: 338.961 bits (868), Expect = 5.581e-105 Identity = 166/398 (41.71%), Postives = 251/398 (63.07%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQL--VGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDL 1353
+TF +D + W + + +PEL + +HR+ YA ++TA+Y++LLRGA+ L +RK V +EFGGG R F+ +E ++++V E F TSQERQSI+++WL +LRA+ + + L++Q I+P L ++ I Q+FP+H IL L + WV QPL++I YFGVKIA+YFAWLG YT A++ P + G + + Q + D+ + ALFN++W+T +L+ WKR +E YKWGT+DS E + E RP F+G I P+TR + YYP WKR F VSLP+ C++CV L+ FQ+ + + K + PK++LA ++ + +KK+A+ LN MEN+RLE +Y HL+ K++LFQF+NS+ SLFYI FYL+D+
Sbjct: 69 MTFPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTRGFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFPVHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSRDVSCVVFALFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGVRRISPITRAEEFYYPPWKRLLFQLLVSLPLCLACLVCVF--LLMLGCFQL-QELVLSVKGLPRLARFLPKVMLALLVSVSAEGYKKLAIWLNDMENYRLESAYEKHLIIKVVLFQFVNSYLSLFYIGFYLKDM 463
BLAST of Anoctamin vs. Ensembl Human
Match: ANO8 (anoctamin 8 [Source:HGNC Symbol;Acc:HGNC:29329]) HSP 1 Score: 338.961 bits (868), Expect = 5.581e-105 Identity = 166/398 (41.71%), Postives = 251/398 (63.07%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQL--VGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDL 1353
+TF +D + W + + +PEL + +HR+ YA ++TA+Y++LLRGA+ L +RK V +EFGGG R F+ +E ++++V E F TSQERQSI+++WL +LRA+ + + L++Q I+P L ++ I Q+FP+H IL L + WV QPL++I YFGVKIA+YFAWLG YT A++ P + G + + Q + D+ + ALFN++W+T +L+ WKR +E YKWGT+DS E + E RP F+G I P+TR + YYP WKR F VSLP+ C++CV L+ FQ+ + + K + PK++LA ++ + +KK+A+ LN MEN+RLE +Y HL+ K++LFQF+NS+ SLFYI FYL+D+
Sbjct: 69 MTFPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTRGFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFPVHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSRDVSCVVFALFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGVRRISPITRAEEFYYPPWKRLLFQLLVSLPLCLACLVCVF--LLMLGCFQL-QELVLSVKGLPRLARFLPKVMLALLVSVSAEGYKKLAIWLNDMENYRLESAYEKHLIIKVVLFQFVNSYLSLFYIGFYLKDM 463
BLAST of Anoctamin vs. Ensembl Human
Match: ANO10 (anoctamin 10 [Source:HGNC Symbol;Acc:HGNC:25519]) HSP 1 Score: 236.884 bits (603), Expect = 9.593e-67 Identity = 155/413 (37.53%), Postives = 231/413 (55.93%), Query Frame = 1
Query: 286 LYLTASYK-NLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKN--WYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLE 1503
LYL + K +L GAE + + K+ + F D D FLT E Q I+K+ L +LRA++E G K +S++ L++ + Q+FPLH+ LK L + W T+F QP++ I YFG IALYF +L ++T AL+ +IGL + L E++D Y +F A FN++W+T L+ WKR C+ TY+WGT+ + + E RP F G + I +T ++ YP++KRQ +Y VSLP + LC+ + V +I+ +VW E W S+L P I+ A VI M+ +++ A L ENHRLE +Y+NHL+ K+++F F+N F SLFYIAF L+D+ LR+ LATLLI Q + I E +P+ R G+R KRK+ L+
Sbjct: 69 LYLVGASKIRMLLGAEAVGLVKECNDNTMRAFTYRTRQNFKGFDD--NNDDFLTMAECQFIIKHELENLRAKDEKMIPGYPQAKLYPGKSLLRRLLTSGIVIQVFPLHDSEALKKLEDTWYTRFALKYQPIDSIRGYFGETIALYFGFLEYFTFALIPMAVIGL-PYYLFVWEDYDKYVIF-ASFNLIWSTVILELWKRGCANMTYRWGTLLMKRKF-EEPRPGFHGVLGINSITGKEEPLYPSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEVWALGLHENSGSEWTSVLLYVPSIIYAIVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLILKVLVFNFLNCFASLFYIAFVLKDMKLLRQSLATLLITSQILNQIMESFLPYWLQRKH----GVRV-KRKVQALK 471 HSP 2 Score: 108.227 bits (269), Expect = 1.431e-23 Identity = 52/126 (41.27%), Postives = 81/126 (64.29%), Query Frame = 1
Query: 1882 ETTLY-QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIE 2256
+ TLY Q E + Y F+D+LE+F+QFGYVS+F+C +PLA F +LNN E+ SDA K+ ++RPF +IGVWQ+A E +S ++V N L+G+S ++ + + A +I ++VA+E
Sbjct: 475 DATLYEQVILEKEMGTYLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFSEPSANIGVWQLAFETMSVISVVTNCALIGMSPQVNAVFPESKA-DLILIVVAVE 599
BLAST of Anoctamin vs. Ensembl Human
Match: ANO10 (anoctamin 10 [Source:HGNC Symbol;Acc:HGNC:25519]) HSP 1 Score: 235.728 bits (600), Expect = 1.150e-66 Identity = 144/370 (38.92%), Postives = 216/370 (58.38%), Query Frame = 1
Query: 412 QDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKN--WYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLE 1503
+D D FLT E Q I+K+ L +LRA++E G K +S++ L++ + Q+FPLH+ LK L + W T+F QP++ I YFG IALYF +L ++T AL+ +IGL + L E++D Y +F + FN++W+T L+ WKR C+ TY+WGT+ + + E RP F G + I +T ++ YP++KRQ +Y VSLP + LC+ + V +I+ +VW E W S+L P I+ A VI M+ +++ A L ENHRLE +Y+NHL+ K+++F F+N F SLFYIAF L+D+ LR+ LATLLI Q + I E +P+ R G+R KRK+ L+
Sbjct: 44 KDGDNNDDFLTMAECQFIIKHELENLRAKDEKMIPGYPQAKLYPGKSLLRRLLTSGIVIQVFPLHDSEALKKLEDTWYTRFALKYQPIDSIRGYFGETIALYFGFLEYFTFALIPMAVIGL-PYYLFVWEDYDKYVIFAS-FNLIWSTVILELWKRGCANMTYRWGTLLMKRKF-EEPRPGFHGVLGINSITGKEEPLYPSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEVWALGLHENSGSEWTSVLLYVPSIIYAIVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLILKVLVFNFLNCFASLFYIAFVLKDMKLLRQSLATLLITSQILNQIMESFLPYWLQRKH----GVRV-KRKVQALK 405 HSP 2 Score: 133.65 bits (335), Expect = 8.675e-32 Identity = 66/176 (37.50%), Postives = 110/176 (62.50%), Query Frame = 1
Query: 1882 ETTLY-QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRH 2406
+ TLY Q E + Y F+D+LE+F+QFGYVS+F+C +PLA F +LNN E+ SDA K+ ++RPF +IGVWQ+A E +S ++V N L+G+S ++ + + A +I ++VA+EH +L +KF++ +IP+ P I+ + +E + EALK + + + E ++
Sbjct: 409 DATLYEQVILEKEMGTYLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFSEPSANIGVWQLAFETMSVISVVTNCALIGMSPQVNAVFPESKA-DLILIVVAVEHALLALKFILAFAIPDKPRHIQMKLARLEFESLEALKQQQMKLVTENLKE 583
BLAST of Anoctamin vs. Ensembl Celegans
Match: anoh-2 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:H2L2L3]) HSP 1 Score: 455.677 bits (1171), Expect = 8.345e-147 Identity = 276/754 (36.60%), Postives = 406/754 (53.85%), Query Frame = 1
Query: 199 IQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSI---------LKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVG--------------QENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTID-SQIELLTEERPSFKGE-MTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTL--EIMKKCQDRSQRKTIH--KTISTDSVDEMSEFSGNKRP--DFLNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSDCNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
+ W + ++ E+ P L + YHR YA+YLTA YK LL+GAEL +I+K + S+FGGG RDF+ +E F V ++ FL+ ER IVK + +RA K GLS+ + +IVP L+S + + LHN LKHL + WV + QP++++ YFG +IA+YFAWLGH T AL P+++G++ W L G Q DI ++ A FN +W+T YL+ WKR +E +KWGT D +Q L + RP+F+G+ + P++ R +YP WK Y ++ P+ FLCV+ + L + Q D + S L P I A +I D I++++A+ LN +EN+R +D Y + L+TK++LFQF+ +F SLFYIAF L+D+ +L+E LATLLI RQ NI E ++PF+ EK KL +L ++ + D + R+ + + +SV++ E + P F + P E + P S G + +L +L S ++ ++ R +P+ K DS L QAE E+ + Y P +DFLEMFIQFGYV +F+ AFPLA + L+NN++EIR DAFKL ++ QRPFG V IG WQ A+E L + V VN L+G SG + RI S I +IV +EHV+L K +I+ +P+VP+W+ E EH RREA K
Sbjct: 57 VMWLVQQMREHEPNLRIEVRYHRLNSCYAIYLTADYKTLLKGAELCHIKKAIKSKFGGGMRDFSFEEAQFFSGVEGKNTFLSPMERTIIVKQMMDMMRA----GKGGLSLKLPRRTITFTEGTAIVPRLISMNVVQNVSALHNTEFLKHLQQKWVFSI-DEQPIDQVKDYFGTEIAMYFAWLGHMTTALWFPSLLGILMWFLGGFKYKNSPGDKQDLYQLISDICFVLFAFFNCIWSTIYLEWWKRVQAELAFKWGTYDATQDSYLQDPRPAFQGDYLAPNPVSGRLEPFYPAWKHTVMRYVITYPITFLCVVGMFAAMLAIFTVQDLADFYFGDSWMLSWLCYFPMIFYALMIVISDKIYRRLALILNDLENYRTDDEYEDFLITKIVLFQFVTAFGSLFYIAFVLRDMKRLQETLATLLITRQVTQNIMETVVPFM------------IEKLKLSSLTYKMTRSMSDGTLRRHVENVRNKRQNSVEQEEEEKSPRTPTTSFFTLGSPTT-PETEVRQRKPNSDGTLATGDFKQLRTLSSI-------------FREDFSLKTERLPLPEFKPCTDS--------------NPELTQAELESVMSVYARPLDDFLEMFIQFGYVLLFSPAFPLAAVCALINNLIEIRVDAFKLCNTVQRPFGRRVKDIGAWQKAMEILGILGVIVNCALIGQSGLVQRIWPDLSWGGQILIIVVLEHVILASKMIIDILVPDVPHWVRIETAKQEHFRREAFK 765
BLAST of Anoctamin vs. Ensembl Celegans
Match: anoh-2 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:H2L2L3]) HSP 1 Score: 455.677 bits (1171), Expect = 4.205e-146 Identity = 276/754 (36.60%), Postives = 406/754 (53.85%), Query Frame = 1
Query: 199 IQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSI---------LKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVG--------------QENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTID-SQIELLTEERPSFKGE-MTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTL--EIMKKCQDRSQRKTIH--KTISTDSVDEMSEFSGNKRP--DFLNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSDCNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
+ W + ++ E+ P L + YHR YA+YLTA YK LL+GAEL +I+K + S+FGGG RDF+ +E F V ++ FL+ ER IVK + +RA K GLS+ + +IVP L+S + + LHN LKHL + WV + QP++++ YFG +IA+YFAWLGH T AL P+++G++ W L G Q DI ++ A FN +W+T YL+ WKR +E +KWGT D +Q L + RP+F+G+ + P++ R +YP WK Y ++ P+ FLCV+ + L + Q D + S L P I A +I D I++++A+ LN +EN+R +D Y + L+TK++LFQF+ +F SLFYIAF L+D+ +L+E LATLLI RQ NI E ++PF+ EK KL +L ++ + D + R+ + + +SV++ E + P F + P E + P S G + +L +L S ++ ++ R +P+ K DS L QAE E+ + Y P +DFLEMFIQFGYV +F+ AFPLA + L+NN++EIR DAFKL ++ QRPFG V IG WQ A+E L + V VN L+G SG + RI S I +IV +EHV+L K +I+ +P+VP+W+ E EH RREA K
Sbjct: 113 VMWLVQQMREHEPNLRIEVRYHRLNSCYAIYLTADYKTLLKGAELCHIKKAIKSKFGGGMRDFSFEEAQFFSGVEGKNTFLSPMERTIIVKQMMDMMRA----GKGGLSLKLPRRTITFTEGTAIVPRLISMNVVQNVSALHNTEFLKHLQQKWVFSI-DEQPIDQVKDYFGTEIAMYFAWLGHMTTALWFPSLLGILMWFLGGFKYKNSPGDKQDLYQLISDICFVLFAFFNCIWSTIYLEWWKRVQAELAFKWGTYDATQDSYLQDPRPAFQGDYLAPNPVSGRLEPFYPAWKHTVMRYVITYPITFLCVVGMFAAMLAIFTVQDLADFYFGDSWMLSWLCYFPMIFYALMIVISDKIYRRLALILNDLENYRTDDEYEDFLITKIVLFQFVTAFGSLFYIAFVLRDMKRLQETLATLLITRQVTQNIMETVVPFM------------IEKLKLSSLTYKMTRSMSDGTLRRHVENVRNKRQNSVEQEEEEKSPRTPTTSFFTLGSPTT-PETEVRQRKPNSDGTLATGDFKQLRTLSSI-------------FREDFSLKTERLPLPEFKPCTDS--------------NPELTQAELESVMSVYARPLDDFLEMFIQFGYVLLFSPAFPLAAVCALINNLIEIRVDAFKLCNTVQRPFGRRVKDIGAWQKAMEILGILGVIVNCALIGQSGLVQRIWPDLSWGGQILIIVVLEHVILASKMIIDILVPDVPHWVRIETAKQEHFRREAFK 821
BLAST of Anoctamin vs. Ensembl Celegans
Match: anoh-1 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:C0P286]) HSP 1 Score: 92.8189 bits (229), Expect = 5.850e-19 Identity = 93/359 (25.91%), Postives = 150/359 (41.78%), Query Frame = 1
Query: 655 QPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLI---------------GWQ------LVGQENH---------DIYY----------------LFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKG-EMTICPLTRRQIRYYPNWKR-----QSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIE---EKNWYSILSPT--PKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFY-----------------------LQDLVK--LREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKR 1485
QPLN+I YFG +IA YFAW G + L I GL+ W +GQ + +++ F A+F +W + +++ WKR S +Y+W + D + +RP F+G ++ P+T I P R SFV FVS ++ + V+ ++ V+L+ ++W+ N + E ++ LS P IL + I+ + +LN ENHR E + N L+ K+ FQ +N++ SLFY+AF L D L QL T +I+ F K++++P+ R + R E R
Sbjct: 250 QPLNKIRDYFGEQIAYYFAWQGTFLTLLWPAVIFGLVVFIYGFIDSISSAPLDWNHCKVVNFIGQTENVACGMRNGVTLFFSMVTQWFMSSFDTKMNAFFAVFMSIWGSVFVQIWKRNNSVLSYQWNSDD--FHAIEPDRPEFRGSKVKEDPITGEDIWISPALARYIKMLASFV-FVSFSML-VVVISLMLVTLL----KIWMVYNFQCTKEYTFHCWLSAAFLPSILNTLSAMGLGAIYSNLVSRLNSWENHRTESEHNNSLIVKIFAFQMVNTYTSLFYVAFIRPESHGLQPNGLFGLGTEFKDTCLDDTCSSLLALQLLTHTLIKPFPKFFKDVVLPYFVKLFRLRMYTSRTEAR 600 HSP 2 Score: 51.2174 bits (121), Expect = 3.616e-6 Identity = 43/154 (27.92%), Postives = 72/154 (46.75%), Query Frame = 1
Query: 1954 EMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVIT----LIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIR 2403
E I FG MFA FPLA L L+ +++R DA +LI ++P IG+W L L Y AV N ++ + G A + +++ +++V +K+L++ IP++P I ++ + KR +EK + R R
Sbjct: 631 EKIILFGTTMMFASLFPLAPLLALIIGFVDMRIDAHRLIWFNRKPIPIITNGIGIWLPILTFLQYCAVFTNAFIVAFTSGFCSTFLADGAYCTVQNRLIIVIVFQNLVFGLKYLLSSVIPSIPASI---KLALRKKRYVVAHIVEKGDVPHRTR 781
BLAST of Anoctamin vs. Ensembl Celegans
Match: anoh-1 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:C0P286]) HSP 1 Score: 92.8189 bits (229), Expect = 5.963e-19 Identity = 92/359 (25.63%), Postives = 147/359 (40.95%), Query Frame = 1
Query: 655 QPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLI---------------GWQ------LVGQENH---------DIYY----------------LFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKG-EMTICPLTRRQIRYYPNWKR-----QSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIE---EKNWYSILSPT--PKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFY-----------------------LQDLVK--LREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKR 1485
QPLN+I YFG +IA YFAW G + L I GL+ W +GQ + +++ F A+F +W + +++ WKR S +Y+W + D + +RP F+G ++ P+T I P R SFV+ V F ++ VI + L+ + ++W+ N + E ++ LS P IL + I+ + +LN ENHR E + N L+ K+ FQ +N++ SLFY+AF L D L QL T +I+ F K++++P+ R + R E R
Sbjct: 268 QPLNKIRDYFGEQIAYYFAWQGTFLTLLWPAVIFGLVVFIYGFIDSISSAPLDWNHCKVVNFIGQTENVACGMRNGVTLFFSMVTQWFMSSFDTKMNAFFAVFMSIWGSVFVQIWKRNNSVLSYQWNSDD--FHAIEPDRPEFRGSKVKEDPITGEDIWISPALARYIKMLASFVF-----VSFSMLVVVISLMLV-TLLKIWMVYNFQCTKEYTFHCWLSAAFLPSILNTLSAMGLGAIYSNLVSRLNSWENHRTESEHNNSLIVKIFAFQMVNTYTSLFYVAFIRPESHGLQPNGLFGLGTEFKDTCLDDTCSSLLALQLLTHTLIKPFPKFFKDVVLPYFVKLFRLRMYTSRTEAR 618 HSP 2 Score: 51.2174 bits (121), Expect = 3.641e-6 Identity = 43/154 (27.92%), Postives = 72/154 (46.75%), Query Frame = 1
Query: 1954 EMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVIT----LIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIR 2403
E I FG MFA FPLA L L+ +++R DA +LI ++P IG+W L L Y AV N ++ + G A + +++ +++V +K+L++ IP++P I ++ + KR +EK + R R
Sbjct: 649 EKIILFGTTMMFASLFPLAPLLALIIGFVDMRIDAHRLIWFNRKPIPIITNGIGIWLPILTFLQYCAVFTNAFIVAFTSGFCSTFLADGAYCTVQNRLIIVIVFQNLVFGLKYLLSSVIPSIPASI---KLALRKKRYVVAHIVEKGDVPHRTR 799
BLAST of Anoctamin vs. Ensembl Fly
Match: CG15270 (gene:FBgn0028879 transcript:FBtr0080703) HSP 1 Score: 308.145 bits (788), Expect = 1.268e-88 Identity = 175/437 (40.05%), Postives = 250/437 (57.21%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPE---LSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQ----DVSEEDIFLTSQERQSIVKYWLYSLRARN---ETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV---GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRR-QIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF 1434
+ F + + ++W + + P L + H + A Y++A L + AE + K++ + GG R+F E FQ DV +F TSQERQ +V L LRA E ++ + QSIV I+Q+FPLH + L L WV + PQPL++IA+YFGVK+ALYFAWLGHYT AL +P + G I + ++ GQ D+ ++ +LFN+ WA+ YL+ WKR E ++WGT+ + ELL RP +KG + +T R + + P W+R++F Y VS P+I C+ V V + FQ W D + E++ LS PK+LLA I MD+ + K+AV LN EN+RL+ Y NHL+ K+ LFQF+NSF SLFYIAFYL+D KL+EQLA LLI RQ IGN++E +P+
Sbjct: 112 IEFPEDAPDEALRWLLARIRSQPPAGLGLLVQVKAHESTRRNAFYVSAPVNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLRGDVGSTALF-TSQERQWLVLQVLQGLRAGCSDIEALHGRAAVAEGQSIVATWQESGLITQVFPLHEPSSLTQLQTHWVKQIFAPQPLDDIAAYFGVKVALYFAWLGHYTCALGVPAVFGTILYCILWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGPLEENNVTGRLEPKEAPAWQRRAFRYLVSFPIIGCCLCVVFAVMFLMLRFQDWWDSKLPEESVLCCLSVIPKVLLAGAITLMDEAYFKLAVWLNDRENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEEKLKEQLAGLLISRQIIGNLRESAIPY 547 HSP 2 Score: 154.066 bits (388), Expect = 8.612e-38 Identity = 76/154 (49.35%), Postives = 101/154 (65.58%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRRE 2358
QAE E++L KYD F D LEM +Q GYV +F+ AFPLAG+ L+NN++EIRSDAFKL +QRPFG V +IG WQ AL LS AV VN L+G+SG +SR+ + I LIV +EH++L ++ + +P +P W+ E EH RRE
Sbjct: 610 QAEIESSLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGVCALINNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNALSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRQALTWLLPELPSWLAAEIARAEHCRRE 763
BLAST of Anoctamin vs. Ensembl Fly
Match: CG15270 (gene:FBgn0028879 transcript:FBtr0344792) HSP 1 Score: 308.145 bits (788), Expect = 1.879e-88 Identity = 175/437 (40.05%), Postives = 250/437 (57.21%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPE---LSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQ----DVSEEDIFLTSQERQSIVKYWLYSLRARN---ETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV---GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRR-QIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF 1434
+ F + + ++W + + P L + H + A Y++A L + AE + K++ + GG R+F E FQ DV +F TSQERQ +V L LRA E ++ + QSIV I+Q+FPLH + L L WV + PQPL++IA+YFGVK+ALYFAWLGHYT AL +P + G I + ++ GQ D+ ++ +LFN+ WA+ YL+ WKR E ++WGT+ + ELL RP +KG + +T R + + P W+R++F Y VS P+I C+ V V + FQ W D + E++ LS PK+LLA I MD+ + K+AV LN EN+RL+ Y NHL+ K+ LFQF+NSF SLFYIAFYL+D KL+EQLA LLI RQ IGN++E +P+
Sbjct: 112 IEFPEDAPDEALRWLLARIRSQPPAGLGLLVQVKAHESTRRNAFYVSAPVNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLRGDVGSTALF-TSQERQWLVLQVLQGLRAGCSDIEALHGRAAVAEGQSIVATWQESGLITQVFPLHEPSSLTQLQTHWVKQIFAPQPLDDIAAYFGVKVALYFAWLGHYTCALGVPAVFGTILYCILWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGPLEENNVTGRLEPKEAPAWQRRAFRYLVSFPIIGCCLCVVFAVMFLMLRFQDWWDSKLPEESVLCCLSVIPKVLLAGAITLMDEAYFKLAVWLNDRENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEEKLKEQLAGLLISRQIIGNLRESAIPY 547 HSP 2 Score: 154.451 bits (389), Expect = 8.112e-38 Identity = 76/154 (49.35%), Postives = 101/154 (65.58%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRRE 2358
QAE E++L KYD F D LEM +Q GYV +F+ AFPLAG+ L+NN++EIRSDAFKL +QRPFG V +IG WQ AL LS AV VN L+G+SG +SR+ + I LIV +EH++L ++ + +P +P W+ E EH RRE
Sbjct: 610 QAEIESSLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGVCALINNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNALSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRQALTWLLPELPSWLAAEIARAEHCRRE 763
BLAST of Anoctamin vs. Ensembl Fly
Match: CG15270 (gene:FBgn0028879 transcript:FBtr0344791) HSP 1 Score: 307.76 bits (787), Expect = 2.339e-88 Identity = 175/437 (40.05%), Postives = 250/437 (57.21%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPE---LSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQ----DVSEEDIFLTSQERQSIVKYWLYSLRARN---ETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV---GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRR-QIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF 1434
+ F + + ++W + + P L + H + A Y++A L + AE + K++ + GG R+F E FQ DV +F TSQERQ +V L LRA E ++ + QSIV I+Q+FPLH + L L WV + PQPL++IA+YFGVK+ALYFAWLGHYT AL +P + G I + ++ GQ D+ ++ +LFN+ WA+ YL+ WKR E ++WGT+ + ELL RP +KG + +T R + + P W+R++F Y VS P+I C+ V V + FQ W D + E++ LS PK+LLA I MD+ + K+AV LN EN+RL+ Y NHL+ K+ LFQF+NSF SLFYIAFYL+D KL+EQLA LLI RQ IGN++E +P+
Sbjct: 112 IEFPEDAPDEALRWLLARIRSQPPAGLGLLVQVKAHESTRRNAFYVSAPVNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLRGDVGSTALF-TSQERQWLVLQVLQGLRAGCSDIEALHGRAAVAEGQSIVATWQESGLITQVFPLHEPSSLTQLQTHWVKQIFAPQPLDDIAAYFGVKVALYFAWLGHYTCALGVPAVFGTILYCILWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGPLEENNVTGRLEPKEAPAWQRRAFRYLVSFPIIGCCLCVVFAVMFLMLRFQDWWDSKLPEESVLCCLSVIPKVLLAGAITLMDEAYFKLAVWLNDRENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEEKLKEQLAGLLISRQIIGNLRESAIPY 547 HSP 2 Score: 154.066 bits (388), Expect = 8.686e-38 Identity = 76/154 (49.35%), Postives = 101/154 (65.58%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRRE 2358
QAE E++L KYD F D LEM +Q GYV +F+ AFPLAG+ L+NN++EIRSDAFKL +QRPFG V +IG WQ AL LS AV VN L+G+SG +SR+ + I LIV +EH++L ++ + +P +P W+ E EH RRE
Sbjct: 610 QAEIESSLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGVCALINNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNALSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRQALTWLLPELPSWLAAEIARAEHCRRE 763
BLAST of Anoctamin vs. Ensembl Fly
Match: CG15270 (gene:FBgn0028879 transcript:FBtr0080702) HSP 1 Score: 308.145 bits (788), Expect = 2.703e-88 Identity = 175/437 (40.05%), Postives = 250/437 (57.21%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPE---LSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQ----DVSEEDIFLTSQERQSIVKYWLYSLRARN---ETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV---GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRR-QIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF 1434
+ F + + ++W + + P L + H + A Y++A L + AE + K++ + GG R+F E FQ DV +F TSQERQ +V L LRA E ++ + QSIV I+Q+FPLH + L L WV + PQPL++IA+YFGVK+ALYFAWLGHYT AL +P + G I + ++ GQ D+ ++ +LFN+ WA+ YL+ WKR E ++WGT+ + ELL RP +KG + +T R + + P W+R++F Y VS P+I C+ V V + FQ W D + E++ LS PK+LLA I MD+ + K+AV LN EN+RL+ Y NHL+ K+ LFQF+NSF SLFYIAFYL+D KL+EQLA LLI RQ IGN++E +P+
Sbjct: 112 IEFPEDAPDEALRWLLARIRSQPPAGLGLLVQVKAHESTRRNAFYVSAPVNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLRGDVGSTALF-TSQERQWLVLQVLQGLRAGCSDIEALHGRAAVAEGQSIVATWQESGLITQVFPLHEPSSLTQLQTHWVKQIFAPQPLDDIAAYFGVKVALYFAWLGHYTCALGVPAVFGTILYCILWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGPLEENNVTGRLEPKEAPAWQRRAFRYLVSFPIIGCCLCVVFAVMFLMLRFQDWWDSKLPEESVLCCLSVIPKVLLAGAITLMDEAYFKLAVWLNDRENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEEKLKEQLAGLLISRQIIGNLRESAIPY 547 HSP 2 Score: 154.451 bits (389), Expect = 7.882e-38 Identity = 76/154 (49.35%), Postives = 101/154 (65.58%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRRE 2358
QAE E++L KYD F D LEM +Q GYV +F+ AFPLAG+ L+NN++EIRSDAFKL +QRPFG V +IG WQ AL LS AV VN L+G+SG +SR+ + I LIV +EH++L ++ + +P +P W+ E EH RRE
Sbjct: 610 QAEIESSLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGVCALINNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNALSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRQALTWLLPELPSWLAAEIARAEHCRRE 763
BLAST of Anoctamin vs. Ensembl Fly
Match: CG15270 (gene:FBgn0028879 transcript:FBtr0307486) HSP 1 Score: 299.286 bits (765), Expect = 2.067e-85 Identity = 178/459 (38.78%), Postives = 254/459 (55.34%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPE---LSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQ----DVSEEDIFLTSQERQSIVKYWLYSLRARN---ETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV---GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRR-QIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWID------KNIEE------KNWYS----------ILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF 1434
+ F + + ++W + + P L + H + A Y++A L + AE + K++ + GG R+F E FQ DV +F TSQERQ +V L LRA E ++ + QSIV I+Q+FPLH + L L WV + PQPL++IA+YFGVK+ALYFAWLGHYT AL +P + G I + ++ GQ D+ ++ +LFN+ WA+ YL+ WKR E ++WGT+ + ELL RP +KG + +T R + + P W+R++F Y VS P+I C+ V V + FQ W+D K I + K+W+ LS PK+LLA I MD+ + K+AV LN EN+RL+ Y NHL+ K+ LFQF+NSF SLFYIAFYL+D KL+EQLA LLI RQ IGN++E +P+
Sbjct: 112 IEFPEDAPDEALRWLLARIRSQPPAGLGLLVQVKAHESTRRNAFYVSAPVNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLRGDVGSTALF-TSQERQWLVLQVLQGLRAGCSDIEALHGRAAVAEGQSIVATWQESGLITQVFPLHEPSSLTQLQTHWVKQIFAPQPLDDIAAYFGVKVALYFAWLGHYTCALGVPAVFGTILYCILWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGPLEENNVTGRLEPKEAPAWQRRAFRYLVSFPIIGCCLCVVFAVMFLMLRFQDWLDHHNIPDKGIAQCMYNLHKDWWDSKLPEESVLCCLSVIPKVLLAGAITLMDEAYFKLAVWLNDRENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEEKLKEQLAGLLISRQIIGNLRESAIPY 569 HSP 2 Score: 154.066 bits (388), Expect = 8.840e-38 Identity = 76/154 (49.35%), Postives = 101/154 (65.58%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRRE 2358
QAE E++L KYD F D LEM +Q GYV +F+ AFPLAG+ L+NN++EIRSDAFKL +QRPFG V +IG WQ AL LS AV VN L+G+SG +SR+ + I LIV +EH++L ++ + +P +P W+ E EH RRE
Sbjct: 632 QAEIESSLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGVCALINNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNALSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRQALTWLLPELPSWLAAEIARAEHCRRE 785
BLAST of Anoctamin vs. Ensembl Zebrafish
Match: BX279524.2 (anoctamin 8b [Source:NCBI gene;Acc:562782]) HSP 1 Score: 523.472 bits (1347), Expect = 1.550e-170 Identity = 292/750 (38.93%), Postives = 435/750 (58.00%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR--YEKRKLDTLEIMKKCQDRSQRKTIHKTISTDSV--DEMSEFSGNKRPDF------LNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSDCNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGET-TLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
+TF +D + W + + +PEL + +H++ YA ++TA+Y+NLLRGAE + ++K V EFGGG R F+ +E +++++ E F TSQERQSI+KYWL +LRA++ + + L+ Q I+P L ++ I Q+FPLH IL L + WV QPL++I YFGVKIA+YFAWLG YT ++L P +IG + W L Q + DI + ALFN++WAT +L+ WKR +E YKWGT+++ E L E RP F+G P+T + YYP W+R+ F + VS PV LC+ V L+ Q ++ + K + PKI+LA + D++++KIA LN MEN+RL+ +Y +L+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLIIRQF+ N+KE++ P++ R R + +R +E L+ + R+Q T+ + +M E D ++ E E +S V +S EE S T E G ++V + D +N E + L ++ +S+ S + ET TL QAE E + Y D F+D+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL +S QRPFG V SIG WQ +E + +A+ VN L+G G L R+ S I IV +EH +L+K++I+ +IP++P W+ +E +E +R+EA K E
Sbjct: 57 MTFPDTTDDHTLLWLLNHIRLGIPELIIQIRHHKHTRMYAFFVTATYENLLRGAEEMGLQKAVKPEFGGGSRSFSCEEDYIYENIESELCFFTSQERQSIIKYWLDNLRAKHGEALHNIHFLEGQPIIPELRARGVIQQVFPLHEQRILGQLMKSWVQAVCEKQPLDDICDYFGVKIAMYFAWLGFYTTSMLYPAVIGFVLWMLTESDQTSRDICCVVFALFNVVWATLFLERWKRRGAELAYKWGTLNTPAESLEEPRPQFRGVKRCSPVTGCEEFYYPPWRRRVFRWLVSFPVCILCLCFVFLAMLVCFELQEFV---MGIKELPRVARFIPKIMLAITVTACDEVYRKIACWLNDMENYRLQSAYEKNLIIKMVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLIIRQFVQNMKEVLQPYLAERHRLGELTLRAVWELLVSALLKYGRLAAGRAQVSPSDPTVLGPGLRGPQMEENESESLIDCGLKLRKVSFIEKVERKSASCVSPVDDSFLEEGSPTMVERGMDPASVFEMCD------DEDENGAPEMKELAAEPLPTGVENSSSRDSWMDPPEEQETNTLTQAEVEGCMQTYQDTFQDYQEMFVQFGYVVLFSSAFPLAAMCALINNIIEIRSDAFKLCTSLQRPFGLRVASIGQWQTVMEAMGLIAIIVNCYLIGQCGQLQRLFPWLSPEMAIISIVILEHFAVLLKYIIHVAIPDIPSWVGEEMAKLEFQRKEAFKKHE 797
BLAST of Anoctamin vs. Ensembl Zebrafish
Match: ano8a (anoctamin 8a [Source:ZFIN;Acc:ZDB-GENE-070912-583]) HSP 1 Score: 515.768 bits (1327), Expect = 4.699e-168 Identity = 299/828 (36.11%), Postives = 445/828 (53.74%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLE--------------------IMKKCQ---------------DRSQRKTIHKTISTDSVDEMSEFSG--NKRPDFLNISENAEFPILEDISKVPESHGE----ECSFTPS--------ELGSLESAVPSVSDCNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRHRVHSCDALEKKGENDNAHRDSA-VLTRSRS 2493
+TF +D + W + ++ +P++ + H+ YA ++T+S++NLLRGAE + ++K V FGGG R F+ +E D+++++ E F TSQERQ I+KYWL +LRA+ + + L+ Q I+P L+++ I Q+FPLH IL L WV QPL+++ YFGVKIA+YFAWLG YT ++L P +IG + W Q + DI + A+FN++WAT +L+ WKR +E YKWGT+D+ E L E RP F+G P+T + YYP WKR+ F +FVS P+ LC+ V I Q ++ +E K + SI PKILLA + D+++KKIA+ LN MEN+RL+ +Y N+L+ K + FQF+NS+ SLFYI FYL+D+ +L+E LATLLI RQF+ NIKE++ P++ YE KL L I CQ ++ +R+++ + +E+ + +G +++ F E + + E P+ E PS E + + S+R K GE+ N + K D P G T L Q E E+ + Y+D D+ EMFIQFGYV +F+ AFPLA + L+NNI+EIRSDA KL S QRPFG V +IG WQ A+E + +A+ VN L+G G L R+ S I +V +EH +L+K++I+ +IP+VP W+ E +E++RREALK E+ + + + R +K E D H+ A L R +S
Sbjct: 40 MTFPDTTDDHTLLWLLNQIRLGIPQIRIQIRQHKYTHTYAFFITSSFENLLRGAEQMGMQKAVKQRFGGGTRRFSCEEDDIYENIESELCFFTSQERQGIIKYWLDNLRAKQGEALHNIHFLEGQPIIPELIARRVILQMFPLHEQRILNQLMTSWVQAVCERQPLDDVCDYFGVKIAMYFAWLGFYTNSMLYPAVIGFLLWMFAESDQTSQDICCVVFAIFNVVWATLFLERWKRREAELAYKWGTLDTPAESLEEPRPQFRGVKRHSPVTGCEEFYYPPWKRRMFRWFVSFPICILCLCFVCLAMFICLELQEFV---METKEFPSICRFIPKILLAVTVTVCDEVYKKIALWLNDMENYRLQSTYDNNLILKTVFFQFINSYLSLFYIGFYLKDMERLKEMLATLLIFRQFLQNIKEVLQPYL------------YEHHKLGALRTLWDLIQTMLLNYSHLIVQRIRLTCQASLDMNKNGTPPEQSEKRERRSLISSYRVPKTEEVDDDAGLKHRKVSFTEKVEYKDKAVEEPTQGSPQDDDSPTLVEEGMDPSLIFDIRDDEDDDEDDDREIDDSASKSQRRKGKAAGGEN--NTMEKKKSWMDPPEET---------GSTVLTQPEIESCMLIYEDTLHDYQEMFIQFGYVVLFSSAFPLAAMCALINNIVEIRSDALKLCSGLQRPFGQRVENIGQWQTAMEAMGLIAIIVNCYLIGQCGQLQRLFPWLSPEMAIVSVVVLEHFAILLKYVIHVAIPDVPNWVADEMAKLEYQRREALKKHERLAQQHQQQKR--------RKTEQDEEHQKLAEELARQQS 833
BLAST of Anoctamin vs. Ensembl Zebrafish
Match: BX279524.2 (anoctamin 8b [Source:NCBI gene;Acc:562782]) HSP 1 Score: 516.153 bits (1328), Expect = 7.281e-167 Identity = 295/799 (36.92%), Postives = 441/799 (55.19%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR------------YEKRKLDTLEIM------------------KKCQDRSQRKTIHKTISTDSVDEMSEFSGNKRPDF----------------LNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSD----------CNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGE----TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
+TF +D + W + + +PEL + +H++ YA ++TA+Y+NLLRGAE + ++K V EFGGG R F+ +E +++++ E F TSQERQSI+KYWL +LRA++ + + L+ Q I+P L ++ I Q+FPLH IL L + WV QPL++I YFGVKIA+YFAWLG YT ++L P +IG + W L Q + DI + ALFN++WAT +L+ WKR +E YKWGT+++ E L E RP F+G P+T + YYP W+R+ F + VS PV LC+ V L+ Q ++ + K + PKI+LA + D++++KIA LN MEN+RL+ +Y +L+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLIIRQF+ N+KE++ P++ R R + +R Y + ++ + QDR +RK ++ D M E G + + ++ E E +S V +S EE S T E G ++V + D ++ L ++ S K+ S + +SK + P E TL QAE E + Y D F+D+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL +S QRPFG V SIG WQ +E + +A+ VN L+G G L R+ S I IV +EH +L+K++I+ +IP++P W+ +E +E +R+EA K E
Sbjct: 67 MTFPDTTDDHTLLWLLNHIRLGIPELIIQIRHHKHTRMYAFFVTATYENLLRGAEEMGLQKAVKPEFGGGSRSFSCEEDYIYENIESELCFFTSQERQSIIKYWLDNLRAKHGEALHNIHFLEGQPIIPELRARGVIQQVFPLHEQRILGQLMKSWVQAVCEKQPLDDICDYFGVKIAMYFAWLGFYTTSMLYPAVIGFVLWMLTESDQTSRDICCVVFALFNVVWATLFLERWKRRGAELAYKWGTLNTPAESLEEPRPQFRGVKRCSPVTGCEEFYYPPWRRRVFRWLVSFPVCILCLCFVFLAMLVCFELQEFV---MGIKELPRVARFIPKIMLAITVTACDEVYRKIACWLNDMENYRLQSAYEKNLIIKMVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLIIRQFVQNMKEVLQPYLAERHRLGELTLRAVWELLVSALLKYGRLAAGRAQVSPSDPTVLGPGLRGPQMGEEGHQDRRERKCLNGGCGVPDED-MEEEHGERHSEEENESESLIDCGLKLRKVSFIEKVERKSASCVSPVDDSFLEEGSPTMVERGMDPASVFEMCDDEDENGAPEMKELAAEPLPTGVENSSSV-KLRKRGRSLERADSKTKRDSWMDPPEEQETNTLTQAEVEGCMQTYQDTFQDYQEMFVQFGYVVLFSSAFPLAAMCALINNIIEIRSDAFKLCTSLQRPFGLRVASIGQWQTVMEAMGLIAIIVNCYLIGQCGQLQRLFPWLSPEMAIISIVILEHFAVLLKYIIHVAIPDIPSWVGEEMAKLEFQRKEAFKKHE 860
BLAST of Anoctamin vs. Ensembl Zebrafish
Match: ano8b (anoctamin 8b [Source:ZFIN;Acc:ZDB-GENE-121024-1]) HSP 1 Score: 516.538 bits (1329), Expect = 9.596e-167 Identity = 295/799 (36.92%), Postives = 441/799 (55.19%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR------------YEKRKLDTLEIM------------------KKCQDRSQRKTIHKTISTDSVDEMSEFSGNKRPDF----------------LNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSD----------CNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGE----TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
+TF +D + W + + +PEL + +H++ YA ++TA+Y+NLLRGAE + ++K V EFGGG R F+ +E +++++ E F TSQERQSI+KYWL +LRA++ + + L+ Q I+P L ++ I Q+FPLH IL L + WV QPL++I YFGVKIA+YFAWLG YT ++L P +IG + W L Q + DI + ALFN++WAT +L+ WKR +E YKWGT+++ E L E RP F+G P+T + YYP W+R+ F + VS PV LC+ V L+ Q ++ + K + PKI+LA + D++++KIA LN MEN+RL+ +Y +L+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLIIRQF+ N+KE++ P++ R R + +R Y + ++ + QDR +RK ++ D M E G + + ++ E E +S V +S EE S T E G ++V + D ++ L ++ S K+ S + +SK + P E TL QAE E + Y D F+D+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL +S QRPFG V SIG WQ +E + +A+ VN L+G G L R+ S I IV +EH +L+K++I+ +IP++P W+ +E +E +R+EA K E
Sbjct: 90 MTFPDTTDDHTLLWLLNHIRLGIPELIIQIRHHKHTRMYAFFVTATYENLLRGAEEMGLQKAVKPEFGGGSRSFSCEEDYIYENIESELCFFTSQERQSIIKYWLDNLRAKHGEALHNIHFLEGQPIIPELRARGVIQQVFPLHEQRILGQLMKSWVQAVCEKQPLDDICDYFGVKIAMYFAWLGFYTTSMLYPAVIGFVLWMLTESDQTSRDICCVVFALFNVVWATLFLERWKRRGAELAYKWGTLNTPAESLEEPRPQFRGVKRCSPVTGCEEFYYPPWRRRVFRWLVSFPVCILCLCFVFLAMLVCFELQEFV---MGIKELPRVARFIPKIMLAITVTACDEVYRKIACWLNDMENYRLQSAYEKNLIIKMVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLIIRQFVQNMKEVLQPYLAERHRLGELTLRAVWELLVSALLKYGRLAAGRAQVSPSDPTVLGPGLRGPQMGEEGHQDRRERKCLNGGCGVPDED-MEEEHGERHSEEENESESLIDCGLKLRKVSFIEKVERKSASCVSPVDDSFLEEGSPTMVERGMDPASVFEMCDDEDENGAPEMKELAAEPLPTGVENSS-SVKLRKRGRSLERADSKTKRDSWMDPPEEQETNTLTQAEVEGCMQTYQDTFQDYQEMFVQFGYVVLFSSAFPLAAMCALINNIIEIRSDAFKLCTSLQRPFGLRVASIGQWQTVMEAMGLIAIIVNCYLIGQCGQLQRLFPWLSPEMAIISIVILEHFAVLLKYIIHVAIPDIPSWVGEEMAKLEFQRKEAFKKHE 883
BLAST of Anoctamin vs. Ensembl Zebrafish
Match: ano10a (anoctamin 10a [Source:NCBI gene;Acc:566425]) HSP 1 Score: 230.72 bits (587), Expect = 1.165e-64 Identity = 161/450 (35.78%), Postives = 245/450 (54.44%), Query Frame = 1
Query: 232 VPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEF-GGGYRDFNLDELDLFQDV-SEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVW-IDKNIEEKNWY-SILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDT-LEIMKKCQDRSQRKTIHKT 1554
V +L F + + + ++ + LL GAE + + K EF G R F + F+D + + FL+ E Q IVK+ L +LRA+NE G K +SIV L SK + Q FPLHN LK L W K + + QPL++I SYFG + LYF +L ++T AL+ +IG I + L E++D Y LF A+FN++W+T +L+ WKR + Y WGT+ + + E R F G + + P+T R+ YP+ KR +Y VS+P + LC+ V +++ + W I E N+ S+L P I+ A VI M+ +++ A L ENHRLE S++NHL+ K+++F F+N F SLFYIAF +QD+V LR+ LATLLI Q + + E +P+ R R + R + D L ++++ Q ++ T T
Sbjct: 52 VDQLLFPAQDGQKPNPNVFVVGSTLQRLLIGAEDVGLFK----EFQDGTMRGFTYANRESFKDFEGDGEGFLSDAECQYIVKHELDTLRAKNEEYIPGYPKYKLYPGKSIVRRLQSKGVLVQYFPLHNKEDLKRLSFSWYKKIKLSFQPLDDIRSYFGEGLGLYFGFLEYFTFALIPMALIG-IPYYLFDWEDYDKYVLF-AVFNLVWSTVFLEVWKRCSATLAYSWGTLGRK-KAFEEPRAGFHGPLGLNPVTGREEPIYPSSKRHLRIYLVSVPFVLLCLYLSFYVMMVYFDMEFWAISIYHENPNFATSVLLFVPSIIYAVVIEIMNLLYRYAAEFLTDWENHRLESSFQNHLVLKVLVFNFVNCFASLFYIAFVMQDMVLLRQSLATLLITSQILNQVMEAFLPYWLQRRRNKRVYKRMRRLMGDKELPLLEQIQLETEMNTYLGT 494 HSP 2 Score: 126.331 bits (316), Expect = 1.622e-29 Identity = 65/159 (40.88%), Postives = 99/159 (62.26%), Query Frame = 1
Query: 1891 LYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
L Q + E + Y F+D+LE F+ FGYVS+F+C +PLA + +LNNI E+ SDAFK+ ++RPF +IGVWQ+A E +S +AV N L+ +S + + A ++T +VAIEHV+L KF++ IP+VP I+ + ++ + EALK
Sbjct: 479 LEQIQLETEMNTYLGTFDDYLEQFLLFGYVSLFSCVYPLAAVLVVLNNITEVYSDAFKMCHVFKRPFSEPAANIGVWQLAFETMSIIAVVTNCALIALSPQVKAYFPESDAQLILT-VVAIEHVLLAFKFILAFVIPDVPKHIQVKLSKLDFESLEALK 636
BLAST of Anoctamin vs. Ensembl Xenopus
Match: adam22 (ADAM metallopeptidase domain 22 [Source:Xenbase;Acc:XB-GENE-957019]) HSP 1 Score: 518.079 bits (1333), Expect = 3.087e-168 Identity = 297/785 (37.83%), Postives = 435/785 (55.41%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRV-------RQTIMGIRYEKRKLDTLEIMKKCQDRSQRKTIHKTISTDSVDEMSEFSGN------------------------------KRPDFLNISENAEFPILEDISKVPESHGE-----ECSFTPSELGSLESAVPSVSDCNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGE----TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
+TF+ +D + W + + +PEL + +HR+ YA ++TA+Y+NLLRGAE + +RK V +EFGGG R F+ +E ++Q++ E IF TSQERQSI++YW+ +LRA+ S + L+ Q I+P L ++ I Q+FP+H IL L WV QPL+EI YFGVK+A+YF+W+G YT +L+ P + G++ W + Q + DI + ALFN++WAT +L+ WKR +E YKWGT+D+ E + E RP F+G I P+T + YYP WKR F FVSLPV C+ V V L Q +I + K ++ PKILLA ++ D++++KIA LN MEN+RL+ +Y HL+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLL+IRQF+ N+KE+ P + ++ R + +RY ++L K + +T + T + +G KR FL E ED+ VP+ E E S T E GS + SV + + G + + + + KR S + P E T L QAE E+ + KY+D F D+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL + QRPFG V IG WQ +E + +A+ VN L+G G L R++ I IV +EH LL+K++I+ IP++P W+ +E +E++RREA K E
Sbjct: 38 MTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFI---LSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLVIRQFLQNVKEVSQPHLYQKLQRGELTPRSVWLFVRYALKRL----FQKYSLPQHPLRTPGNPMETGAGGRKKCLNGGCGVPEEEEGVVSEDEEEEEEESIMDCGLKLKRVSFLEKGERR-----EDVGLVPQQEDEGAFLQEGSPTLMERGSDPA---SVFQLEDEEDEEQEGASGGVLQEGLEEDEEDDTEMKQKRES--WIDPPEDNYPTKLSQAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHE 805
BLAST of Anoctamin vs. Ensembl Xenopus
Match: adam22 (ADAM metallopeptidase domain 22 [Source:Xenbase;Acc:XB-GENE-957019]) HSP 1 Score: 515.383 bits (1326), Expect = 1.400e-167 Identity = 296/762 (38.85%), Postives = 436/762 (57.22%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRV-------RQTIMGIRYEKRKLDTLEIMKKCQDRSQ----------RKTIHKTISTDSVDEMSEFSGNKRPDFLNISENAEFPILEDISKVPESHGE-----ECSFTPSELGSLESAVPSVSDCNISKRH-LSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
+TF+ +D + W + + +PEL + +HR+ YA ++TA+Y+NLLRGAE + +RK V +EFGGG R F+ +E ++Q++ E IF TSQERQSI++YW+ +LRA+ S + L+ Q I+P L ++ I Q+FP+H IL L WV QPL+EI YFGVK+A+YF+W+G YT +L+ P + G++ W + Q + DI + ALFN++WAT +L+ WKR +E YKWGT+D+ E + E RP F+G I P+T + YYP WKR F FVSLPV C+ V V L Q +I + K ++ PKILLA ++ D++++KIA LN MEN+RL+ +Y HL+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLL+IRQF+ N+KE+ P + ++ R + +RY ++L + + R+ RK + +S+ M KR FL E ED+ VP+ E E S T E GS ++V + D ++ S + E P+ D+ +K S QAE E+ + KY+D F D+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL + QRPFG V IG WQ +E + +A+ VN L+G G L R++ I IV +EH LL+K++I+ IP++P W+ +E +E++RREA K E
Sbjct: 43 MTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFI---LSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLVIRQFLQNVKEVSQPHLYQKLQRGELTPRSVWLFVRYALKRLFQKYSLPQHPLRTPGNPMETGAGGRKKYEEEEEEESI--MDCGLKLKRVSFLEKGERR-----EDVGLVPQQEDEGAFLQEGSPTLMERGSDPASVFQLEDEEDEEQEGASGGVLQEESWIDPPE-----DNYPTKLS-------------QAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHE 776
BLAST of Anoctamin vs. Ensembl Xenopus
Match: adam22 (ADAM metallopeptidase domain 22 [Source:Xenbase;Acc:XB-GENE-957019]) HSP 1 Score: 382.104 bits (980), Expect = 1.191e-116 Identity = 191/451 (42.35%), Postives = 284/451 (62.97%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRV-------RQTIMGIRYEKRKL 1491
+TF+ +D + W + + +PEL + +HR+ YA ++TA+Y+NLLRGAE + +RK V +EFGGG R F+ +E ++Q++ E IF TSQERQSI++YW+ +LRA+ S + L+ Q I+P L ++ I Q+FP+H IL L WV QPL+EI YFGVK+A+YF+W+G YT +L+ P + G++ W + Q + DI + ALFN++WAT +L+ WKR +E YKWGT+D+ E + E RP F+G I P+T + YYP WKR F FVSLPV C+ V V L Q +I + K ++ PKILLA ++ D++++KIA LN MEN+RL+ +Y HL+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLL+IRQF+ N+KE+ P + ++ R + +RY ++L
Sbjct: 36 MTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFI---LSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLVIRQFLQNVKEVSQPHLYQKLQRGELTPRSVWLFVRYALKRL 483 HSP 2 Score: 164.851 bits (416), Expect = 5.727e-41 Identity = 78/164 (47.56%), Postives = 108/164 (65.85%), Query Frame = 1
Query: 1885 TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
T L QAE E+ + KY+D F D+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL + QRPFG V IG WQ +E + +A+ VN L+G G L R++ I IV +EH LL+K++I+ IP++P W+ +E +E++RREA K E
Sbjct: 648 TKLSQAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHE 811
BLAST of Anoctamin vs. Ensembl Xenopus
Match: adam22 (ADAM metallopeptidase domain 22 [Source:Xenbase;Acc:XB-GENE-957019]) HSP 1 Score: 382.874 bits (982), Expect = 1.308e-116 Identity = 191/451 (42.35%), Postives = 284/451 (62.97%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRV-------RQTIMGIRYEKRKL 1491
+TF+ +D + W + + +PEL + +HR+ YA ++TA+Y+NLLRGAE + +RK V +EFGGG R F+ +E ++Q++ E IF TSQERQSI++YW+ +LRA+ S + L+ Q I+P L ++ I Q+FP+H IL L WV QPL+EI YFGVK+A+YF+W+G YT +L+ P + G++ W + Q + DI + ALFN++WAT +L+ WKR +E YKWGT+D+ E + E RP F+G I P+T + YYP WKR F FVSLPV C+ V V L Q +I + K ++ PKILLA ++ D++++KIA LN MEN+RL+ +Y HL+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLL+IRQF+ N+KE+ P + ++ R + +RY ++L
Sbjct: 42 MTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFI---LSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLVIRQFLQNVKEVSQPHLYQKLQRGELTPRSVWLFVRYALKRL 489 HSP 2 Score: 164.851 bits (416), Expect = 6.073e-41 Identity = 78/164 (47.56%), Postives = 108/164 (65.85%), Query Frame = 1
Query: 1885 TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
T L QAE E+ + KY+D F D+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL + QRPFG V IG WQ +E + +A+ VN L+G G L R++ I IV +EH LL+K++I+ IP++P W+ +E +E++RREA K E
Sbjct: 671 TKLSQAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHE 834
BLAST of Anoctamin vs. Ensembl Xenopus
Match: adam22 (ADAM metallopeptidase domain 22 [Source:Xenbase;Acc:XB-GENE-957019]) HSP 1 Score: 382.489 bits (981), Expect = 2.231e-116 Identity = 187/432 (43.29%), Postives = 278/432 (64.35%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQ 1455
+TF+ +D + W + + +PEL + +HR+ YA ++TA+Y+NLLRGAE + +RK V +EFGGG R F+ +E ++Q++ E IF TSQERQSI++YW+ +LRA+ S + L+ Q I+P L ++ I Q+FP+H IL L WV QPL+EI YFGVK+A+YF+W+G YT +L+ P + G++ W + Q + DI + ALFN++WAT +L+ WKR +E YKWGT+D+ E + E RP F+G I P+T + YYP WKR F FVSLPV C+ V V L Q +I + K ++ PKILLA ++ D++++KIA LN MEN+RL+ +Y HL+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLL+IRQF+ N+KE+ P + ++++
Sbjct: 66 MTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFI---LSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLVIRQFLQNVKEVSQPHLYQKLQR 494 HSP 2 Score: 164.851 bits (416), Expect = 5.736e-41 Identity = 78/164 (47.56%), Postives = 108/164 (65.85%), Query Frame = 1
Query: 1885 TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
T L QAE E+ + KY+D F D+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL + QRPFG V IG WQ +E + +A+ VN L+G G L R++ I IV +EH LL+K++I+ IP++P W+ +E +E++RREA K E
Sbjct: 695 TKLSQAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHE 858
BLAST of Anoctamin vs. Ensembl Mouse
Match: Ano8 (anoctamin 8 [Source:MGI Symbol;Acc:MGI:2687327]) HSP 1 Score: 376.711 bits (966), Expect = 1.900e-114 Identity = 184/438 (42.01%), Postives = 279/438 (63.70%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIG--LIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR 1473
+TF +D + W + + +PEL + +HR+ YA ++TA+Y++LLRGA+ L +RK V +EFGGG R F+ +E ++++V E F TSQERQSI+++WL +LRA+ + + L++Q I+P L ++ I Q+FP+H IL L + WV QPL++I YFGVKIA+YFAWLG YT A++ P + G L + Q + D+ + ALFN++W+T +L+ WKR +E YKWGT+DS E + E RP F+G I P+TR + YYP WKR F VSLP+ C++CV L+ FQ+ + + K ++ PK++LA ++ + +KK+AV LN MEN+RLE +Y HL+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLI RQ + N++E++ P + R+ +G+R
Sbjct: 69 MTFPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTRSFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFPVHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSRDVSCVVFALFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGIRRISPITRAEEFYYPPWKRLLFQLLVSLPLCLACLICVF--ILMLGCFQLQ-ELVLSVKGLPRLVRFLPKVMLALLVSVSAEGYKKLAVWLNDMENYRLESTYERHLIIKVVLFQFVNSYLSLFYIGFYLKDMDRLKEMLATLLITRQLLQNVREVLQPHLYRRLGSGELGLR 503 HSP 2 Score: 166.392 bits (420), Expect = 1.462e-41 Identity = 78/159 (49.06%), Postives = 110/159 (69.18%), Query Frame = 1
Query: 1891 LYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
L QAE E+ + KY+D F+D+ EMF+QFGYV +F+ AFPLA L L+NN++EIRSDAFKL + QRPFG V SIG WQ +E + +A+ VN L+G G L R+ S I +V +EH+ LL+K+LI+ +IP++P W+ +E +E++RREA K
Sbjct: 724 LTQAELESCMKKYEDTFQDYQEMFVQFGYVVLFSSAFPLAALCALVNNLIEIRSDAFKLCTGLQRPFGRRVESIGQWQKVMEAMGVLAIVVNCYLIGQCGQLQRLFPWLSPEAAIVSVVVLEHLALLVKYLIHVAIPDIPGWVAEEMAKLEYQRREAFK 882
BLAST of Anoctamin vs. Ensembl Mouse
Match: Ano8 (anoctamin 8 [Source:MGI Symbol;Acc:MGI:2687327]) HSP 1 Score: 352.443 bits (903), Expect = 3.888e-105 Identity = 183/482 (37.97%), Postives = 277/482 (57.47%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIG--LIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDL--------------------------------------------VKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR 1473
+TF +D + W + + +PEL + +HR+ YA ++TA+Y++LLRGA+ L +RK V +EFGGG R F+ +E ++++V E F TSQERQSI+++WL +LRA+ + + L++Q I+P L ++ I Q+FP+H IL L + WV QPL++I YFGVKIA+YFAWLG YT A++ P + G L + Q + D+ + ALFN++W+T +L+ WKR +E YKWGT+DS E + E RP F+G I P+TR + YYP WKR F VSLP+ C++CV L+ FQ+ + + K ++ PK++LA ++ + +KK+AV LN MEN+RLE +Y HL+ K++LFQF+NS+ SLFYI FYL+D+ V + LATLLI RQ + N++E++ P + R+ +G+R
Sbjct: 69 MTFPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTRSFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFPVHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSRDVSCVVFALFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGIRRISPITRAEEFYYPPWKRLLFQLLVSLPLCLACLICVF--ILMLGCFQLQ-ELVLSVKGLPRLVRFLPKVMLALLVSVSAEGYKKLAVWLNDMENYRLESTYERHLIIKVVLFQFVNSYLSLFYIGFYLKDMDRLKELLLLLSLTQSLERQLQAVLVPLAALRFRLLLLSLRGLLLVARAKMLATLLITRQLLQNVREVLQPHLYRRLGSGELGLR 547 HSP 2 Score: 166.392 bits (420), Expect = 1.421e-41 Identity = 78/159 (49.06%), Postives = 110/159 (69.18%), Query Frame = 1
Query: 1891 LYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
L QAE E+ + KY+D F+D+ EMF+QFGYV +F+ AFPLA L L+NN++EIRSDAFKL + QRPFG V SIG WQ +E + +A+ VN L+G G L R+ S I +V +EH+ LL+K+LI+ +IP++P W+ +E +E++RREA K
Sbjct: 768 LTQAELESCMKKYEDTFQDYQEMFVQFGYVVLFSSAFPLAALCALVNNLIEIRSDAFKLCTGLQRPFGRRVESIGQWQKVMEAMGVLAIVVNCYLIGQCGQLQRLFPWLSPEAAIVSVVVLEHLALLVKYLIHVAIPDIPGWVAEEMAKLEYQRREAFK 926
BLAST of Anoctamin vs. Ensembl Mouse
Match: Ano10 (anoctamin 10 [Source:MGI Symbol;Acc:MGI:2143103]) HSP 1 Score: 235.728 bits (600), Expect = 8.014e-68 Identity = 152/410 (37.07%), Postives = 232/410 (56.59%), Query Frame = 1
Query: 286 LYLT-ASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKN--WYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF-----VNTRVRQTIMGIRYE 1479
LYL AS LL GAE + + K+ + F D + + FLT E Q I+K+ L +LRAR+E G K +S++ L++ ++Q+FPLH+ LK L + W T+F QP++ I SYFG IALYF +L ++T AL+ IIGL + L E++D Y +F + FN++W+T L+ WKR C+ TY+WGT+ + + E RP F G + I +T R+ Y ++KRQ +Y VSLP + LC+ + V +I+ + W E+ W S+L P I+ A VI M+ +++ A L ENHRLE +Y+NHL+ K+++F F+N F SLFYIAF L+D+ LR+ LATLLI Q + + E ++P+ RV++ + ++ E
Sbjct: 69 LYLVGASNVRLLLGAEAVGLVKECTDAAMRAFTYGTRHNFKGFHDNNND--FLTMAECQFIIKHELENLRARDEKMIPGYPQAKLYPGKSLMRRLLTSGIVTQVFPLHDTEALKKLEDTWYTRFALKYQPIDSIRSYFGETIALYFGFLEYFTFALIPMAIIGL-PYYLFVWEDYDKYVIFAS-FNLIWSTVILEVWKRGCANMTYRWGTLVMKRQF-EEPRPGFHGVLGINSVTGREEPLYSSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEDWALSLHEDSGSEWTSLLLYVPSIVYAVVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLVLKVLVFNFLNCFASLFYIAFVLKDMKLLRQSLATLLITSQILNQVVESLLPYWLQRKYCARVKRKVQALKSE 473
BLAST of Anoctamin vs. Ensembl Mouse
Match: Ano10 (anoctamin 10 [Source:MGI Symbol;Acc:MGI:2143103]) HSP 1 Score: 237.654 bits (605), Expect = 6.238e-67 Identity = 153/410 (37.32%), Postives = 232/410 (56.59%), Query Frame = 1
Query: 286 LYLT-ASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKN--WYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF-----VNTRVRQTIMGIRYE 1479
LYL AS LL GAE + + K+ + F D + + FLT E Q I+K+ L +LRAR+E G K +S++ L++ ++Q+FPLH+ LK L + W T+F QP++ I SYFG IALYF +L ++T AL+ IIGL + L E++D Y +F A FN++W+T L+ WKR C+ TY+WGT+ + + E RP F G + I +T R+ Y ++KRQ +Y VSLP + LC+ + V +I+ + W E+ W S+L P I+ A VI M+ +++ A L ENHRLE +Y+NHL+ K+++F F+N F SLFYIAF L+D+ LR+ LATLLI Q + + E ++P+ RV++ + ++ E
Sbjct: 69 LYLVGASNVRLLLGAEAVGLVKECTDAAMRAFTYGTRHNFKGFHDNNND--FLTMAECQFIIKHELENLRARDEKMIPGYPQAKLYPGKSLMRRLLTSGIVTQVFPLHDTEALKKLEDTWYTRFALKYQPIDSIRSYFGETIALYFGFLEYFTFALIPMAIIGL-PYYLFVWEDYDKYVIF-ASFNLIWSTVILEVWKRGCANMTYRWGTLVMKRQF-EEPRPGFHGVLGINSVTGREEPLYSSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEDWALSLHEDSGSEWTSLLLYVPSIVYAVVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLVLKVLVFNFLNCFASLFYIAFVLKDMKLLRQSLATLLITSQILNQVVESLLPYWLQRKYCARVKRKVQALKSE 473 HSP 2 Score: 139.428 bits (350), Expect = 1.350e-33 Identity = 68/176 (38.64%), Postives = 112/176 (63.64%), Query Frame = 1
Query: 1882 ETTLY-QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRH 2406
+TTLY Q E + Y F+D+LE+F+QFGYVS+F+C +PLA F +LNN E+ SDA K+ ++RPF SIGVWQ+A E +S ++V N L+G+S ++ + + S ++ ++VA+EH +L +KF++ +IP+ P I+Q+ +E + EALK + + + E ++
Sbjct: 475 DTTLYEQVLLEKEMGTYLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFAEPSASIGVWQLAFETMSVISVVTNCALIGMSPQVNAVFPE-SKTDLVLIVVAVEHALLALKFILAFAIPDKPRHIQQKLARLEFESLEALKQQQMKLVAENLKE 649
BLAST of Anoctamin vs. Ensembl Mouse
Match: Ano10 (anoctamin 10 [Source:MGI Symbol;Acc:MGI:2143103]) HSP 1 Score: 236.498 bits (602), Expect = 7.070e-67 Identity = 153/410 (37.32%), Postives = 232/410 (56.59%), Query Frame = 1
Query: 286 LYLT-ASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKN--WYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF-----VNTRVRQTIMGIRYE 1479
LYL AS LL GAE + + K+ + F D + + FLT E Q I+K+ L +LRAR+E G K +S++ L++ ++Q+FPLH+ LK L + W T+F QP++ I SYFG IALYF +L ++T AL+ IIGL + L E++D Y +F A FN++W+T L+ WKR C+ TY+WGT+ + + E RP F G + I +T R+ Y ++KRQ +Y VSLP + LC+ + V +I+ + W E+ W S+L P I+ A VI M+ +++ A L ENHRLE +Y+NHL+ K+++F F+N F SLFYIAF L+D+ LR+ LATLLI Q + + E ++P+ RV++ + ++ E
Sbjct: 69 LYLVGASNVRLLLGAEAVGLVKECTDAAMRAFTYGTRHNFKGFHDNNND--FLTMAECQFIIKHELENLRARDEKMIPGYPQAKLYPGKSLMRRLLTSGIVTQVFPLHDTEALKKLEDTWYTRFALKYQPIDSIRSYFGETIALYFGFLEYFTFALIPMAIIGL-PYYLFVWEDYDKYVIF-ASFNLIWSTVILEVWKRGCANMTYRWGTLVMKRQF-EEPRPGFHGVLGINSVTGREEPLYSSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEDWALSLHEDSGSEWTSLLLYVPSIVYAVVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLVLKVLVFNFLNCFASLFYIAFVLKDMKLLRQSLATLLITSQILNQVVESLLPYWLQRKYCARVKRKVQALKSE 473 HSP 2 Score: 112.464 bits (280), Expect = 3.831e-25 Identity = 56/137 (40.88%), Postives = 86/137 (62.77%), Query Frame = 1
Query: 1882 ETTLY-QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLIN 2289
+TTLY Q E + Y F+D+LE+F+QFGYVS+F+C +PLA F +LNN E+ SDA K+ ++RPF SIGVWQ+A E +S ++V N L+G+S ++ + + S ++ ++VA+E + L F I
Sbjct: 475 DTTLYEQVLLEKEMGTYLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFAEPSASIGVWQLAFETMSVISVVTNCALIGMSPQVNAVFPE-SKTDLVLIVVAVEVLGLQASFTIG 610
BLAST of Anoctamin vs. UniProt/SwissProt
Match: sp|Q6PB70|ANO8_MOUSE (Anoctamin-8 OS=Mus musculus OX=10090 GN=Ano8 PE=1 SV=3) HSP 1 Score: 376.711 bits (966), Expect = 1.329e-113 Identity = 184/438 (42.01%), Postives = 279/438 (63.70%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIG--LIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR 1473
+TF +D + W + + +PEL + +HR+ YA ++TA+Y++LLRGA+ L +RK V +EFGGG R F+ +E ++++V E F TSQERQSI+++WL +LRA+ + + L++Q I+P L ++ I Q+FP+H IL L + WV QPL++I YFGVKIA+YFAWLG YT A++ P + G L + Q + D+ + ALFN++W+T +L+ WKR +E YKWGT+DS E + E RP F+G I P+TR + YYP WKR F VSLP+ C++CV L+ FQ+ + + K ++ PK++LA ++ + +KK+AV LN MEN+RLE +Y HL+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLI RQ + N++E++ P + R+ +G+R
Sbjct: 69 MTFPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTRSFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFPVHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSRDVSCVVFALFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGIRRISPITRAEEFYYPPWKRLLFQLLVSLPLCLACLICVF--ILMLGCFQLQ-ELVLSVKGLPRLVRFLPKVMLALLVSVSAEGYKKLAVWLNDMENYRLESTYERHLIIKVVLFQFVNSYLSLFYIGFYLKDMDRLKEMLATLLITRQLLQNVREVLQPHLYRRLGSGELGLR 503 HSP 2 Score: 166.392 bits (420), Expect = 1.023e-40 Identity = 78/159 (49.06%), Postives = 110/159 (69.18%), Query Frame = 1
Query: 1891 LYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
L QAE E+ + KY+D F+D+ EMF+QFGYV +F+ AFPLA L L+NN++EIRSDAFKL + QRPFG V SIG WQ +E + +A+ VN L+G G L R+ S I +V +EH+ LL+K+LI+ +IP++P W+ +E +E++RREA K
Sbjct: 724 LTQAELESCMKKYEDTFQDYQEMFVQFGYVVLFSSAFPLAALCALVNNLIEIRSDAFKLCTGLQRPFGRRVESIGQWQKVMEAMGVLAIVVNCYLIGQCGQLQRLFPWLSPEAAIVSVVVLEHLALLVKYLIHVAIPDIPGWVAEEMAKLEYQRREAFK 882
BLAST of Anoctamin vs. UniProt/SwissProt
Match: sp|Q9HCE9|ANO8_HUMAN (Anoctamin-8 OS=Homo sapiens OX=9606 GN=ANO8 PE=1 SV=3) HSP 1 Score: 377.867 bits (969), Expect = 9.985e-113 Identity = 184/438 (42.01%), Postives = 280/438 (63.93%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIG--LIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR 1473
+TF +D + W + + +PEL + +HR+ YA ++TA+Y++LLRGA+ L +RK V +EFGGG R F+ +E ++++V E F TSQERQSI+++WL +LRA+ + + L++Q I+P L ++ I Q+FP+H IL L + WV QPL++I YFGVKIA+YFAWLG YT A++ P + G L + Q + D+ + ALFN++W+T +L+ WKR +E YKWGT+DS E + E RP F+G I P+TR + YYP WKR F VSLP+ C++CV L+ FQ+ + + K + PK++LA ++ + +KK+A+ LN MEN+RLE +Y HL+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLI RQF+ N++E++ P + R+ + +G+R
Sbjct: 69 MTFPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTRGFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFPVHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSRDVSCVVFALFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGVRRISPITRAEEFYYPPWKRLLFQLLVSLPLCLACLVCVF--LLMLGCFQL-QELVLSVKGLPRLARFLPKVMLALLVSVSAEGYKKLAIWLNDMENYRLESAYEKHLIIKVVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLITRQFLQNVREVLQPHLYRRLGRGELGLR 503 HSP 2 Score: 165.622 bits (418), Expect = 2.112e-40 Identity = 79/162 (48.77%), Postives = 110/162 (67.90%), Query Frame = 1
Query: 1891 LYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
L QAE E+ + KY+D F+D+ EMF+QFGYV +F+ AFPLA L L+NN++EIRSDAFKL + QRPFG V SIG WQ +E + +A+ VN L+G G L R+ S I +V +EH LL+K+LI+ +IP++P W+ +E +E++RREA K E
Sbjct: 729 LTQAELESCMKKYEDTFQDYQEMFVQFGYVVLFSSAFPLAALCALVNNLIEIRSDAFKLCTGLQRPFGQRVESIGQWQKVMEAMGVLAIVVNCYLIGQCGQLQRLFPWLSPEAAIVSVVVLEHFALLLKYLIHVAIPDIPGWVAEEMAKLEYQRREAFKRHE 890
BLAST of Anoctamin vs. UniProt/SwissProt
Match: sp|Q8BH79|ANO10_MOUSE (Anoctamin-10 OS=Mus musculus OX=10090 GN=Ano10 PE=1 SV=1) HSP 1 Score: 237.654 bits (605), Expect = 4.364e-66 Identity = 153/410 (37.32%), Postives = 232/410 (56.59%), Query Frame = 1
Query: 286 LYLT-ASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKN--WYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF-----VNTRVRQTIMGIRYE 1479
LYL AS LL GAE + + K+ + F D + + FLT E Q I+K+ L +LRAR+E G K +S++ L++ ++Q+FPLH+ LK L + W T+F QP++ I SYFG IALYF +L ++T AL+ IIGL + L E++D Y +F A FN++W+T L+ WKR C+ TY+WGT+ + + E RP F G + I +T R+ Y ++KRQ +Y VSLP + LC+ + V +I+ + W E+ W S+L P I+ A VI M+ +++ A L ENHRLE +Y+NHL+ K+++F F+N F SLFYIAF L+D+ LR+ LATLLI Q + + E ++P+ RV++ + ++ E
Sbjct: 69 LYLVGASNVRLLLGAEAVGLVKECTDAAMRAFTYGTRHNFKGFHDNNND--FLTMAECQFIIKHELENLRARDEKMIPGYPQAKLYPGKSLMRRLLTSGIVTQVFPLHDTEALKKLEDTWYTRFALKYQPIDSIRSYFGETIALYFGFLEYFTFALIPMAIIGL-PYYLFVWEDYDKYVIF-ASFNLIWSTVILEVWKRGCANMTYRWGTLVMKRQF-EEPRPGFHGVLGINSVTGREEPLYSSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEDWALSLHEDSGSEWTSLLLYVPSIVYAVVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLVLKVLVFNFLNCFASLFYIAFVLKDMKLLRQSLATLLITSQILNQVVESLLPYWLQRKYCARVKRKVQALKSE 473 HSP 2 Score: 139.428 bits (350), Expect = 9.440e-33 Identity = 68/176 (38.64%), Postives = 112/176 (63.64%), Query Frame = 1
Query: 1882 ETTLY-QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRH 2406
+TTLY Q E + Y F+D+LE+F+QFGYVS+F+C +PLA F +LNN E+ SDA K+ ++RPF SIGVWQ+A E +S ++V N L+G+S ++ + + S ++ ++VA+EH +L +KF++ +IP+ P I+Q+ +E + EALK + + + E ++
Sbjct: 475 DTTLYEQVLLEKEMGTYLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFAEPSASIGVWQLAFETMSVISVVTNCALIGMSPQVNAVFPE-SKTDLVLIVVAVEHALLALKFILAFAIPDKPRHIQQKLARLEFESLEALKQQQMKLVAENLKE 649
BLAST of Anoctamin vs. UniProt/SwissProt
Match: sp|Q9NW15|ANO10_HUMAN (Anoctamin-10 OS=Homo sapiens OX=9606 GN=ANO10 PE=1 SV=2) HSP 1 Score: 237.269 bits (604), Expect = 7.008e-66 Identity = 154/413 (37.29%), Postives = 231/413 (55.93%), Query Frame = 1
Query: 286 LYLTASYK-NLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKN--WYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLE 1503
LYL + K +L GAE + + K+ + F D D FLT E Q I+K+ L +LRA++E G K +S++ L++ + Q+FPLH+ LK L + W T+F QP++ I YFG IALYF +L ++T AL+ +IGL + L E++D Y +F + FN++W+T L+ WKR C+ TY+WGT+ + + E RP F G + I +T ++ YP++KRQ +Y VSLP + LC+ + V +I+ +VW E W S+L P I+ A VI M+ +++ A L ENHRLE +Y+NHL+ K+++F F+N F SLFYIAF L+D+ LR+ LATLLI Q + I E +P+ R G+R KRK+ L+
Sbjct: 69 LYLVGASKIRMLLGAEAVGLVKECNDNTMRAFTYRTRQNFKGFDD--NNDDFLTMAECQFIIKHELENLRAKDEKMIPGYPQAKLYPGKSLLRRLLTSGIVIQVFPLHDSEALKKLEDTWYTRFALKYQPIDSIRGYFGETIALYFGFLEYFTFALIPMAVIGL-PYYLFVWEDYDKYVIFAS-FNLIWSTVILELWKRGCANMTYRWGTLLMKRKF-EEPRPGFHGVLGINSITGKEEPLYPSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEVWALGLHENSGSEWTSVLLYVPSIIYAIVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLILKVLVFNFLNCFASLFYIAFVLKDMKLLRQSLATLLITSQILNQIMESFLPYWLQRKH----GVRV-KRKVQALK 471 HSP 2 Score: 133.65 bits (335), Expect = 5.898e-31 Identity = 66/176 (37.50%), Postives = 110/176 (62.50%), Query Frame = 1
Query: 1882 ETTLY-QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRH 2406
+ TLY Q E + Y F+D+LE+F+QFGYVS+F+C +PLA F +LNN E+ SDA K+ ++RPF +IGVWQ+A E +S ++V N L+G+S ++ + + A +I ++VA+EH +L +KF++ +IP+ P I+ + +E + EALK + + + E ++
Sbjct: 475 DATLYEQVILEKEMGTYLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFSEPSANIGVWQLAFETMSVISVVTNCALIGMSPQVNAVFPESKA-DLILIVVAVEHALLALKFILAFAIPDKPRHIQMKLARLEFESLEALKQQQMKLVTENLKE 649
BLAST of Anoctamin vs. UniProt/SwissProt
Match: sp|Q4V8U5|ANO10_DANRE (Anoctamin-10 OS=Danio rerio OX=7955 GN=ano10 PE=2 SV=1) HSP 1 Score: 231.491 bits (589), Expect = 4.502e-64 Identity = 161/450 (35.78%), Postives = 244/450 (54.22%), Query Frame = 1
Query: 232 VPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEF-GGGYRDFNLDELDLFQDV-SEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVW-IDKNIEEKNWY-SILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDT-LEIMKKCQDRSQRKTIHKT 1554
V +L F + + + ++ + LL GAE + + K EF G R F + F+D + + FL E Q IVK+ L +LRA+NE G K +SIV L SK + Q FPLHN LK L W K + + QPL++I SYFG + LYF +L ++T AL+ +IG I + L E++D Y LF A+FN++W+T +L+ WKR + Y WGT+ + + E R F G + + P+T R+ YP+ KR +Y VS+P + LC+ V +++ + W I E N+ S+L P I+ A VI M+ +++ A L ENHRLE S++NHL+ K+++F F+N F SLFYIAF +QD+V LR+ LATLLI Q + + E +P+ R R + R + D L ++++ Q ++ T T
Sbjct: 52 VDQLLFPAQDGQKPNPNVFVVGSTLQRLLSGAEDVGLFK----EFQDGTMRGFTYANRESFKDFEGDGEGFLGDAECQYIVKHELDTLRAKNEEYIPGYPKYKLYPGKSIVRRLQSKGVLVQYFPLHNKEDLKRLSFSWYKKIKLSFQPLDDIRSYFGEGLGLYFGFLEYFTFALIPMALIG-IPYYLFDWEDYDKYVLF-AVFNLVWSTVFLEVWKRCSATLAYSWGTLGRK-KAFEEPRAGFHGPLGLNPVTGREEPIYPSSKRHLRIYLVSVPFVLLCLYLSFYVMMVYFDMEFWAISIYHENPNFATSVLLFVPSIIYAVVIEIMNLLYRYAAEFLTDWENHRLESSFQNHLVPKVLVFNFVNCFASLFYIAFVMQDMVLLRQSLATLLITSQILNQVMEAFLPYWLQRRRNKRVYKRMRRLMGDKELPLLEQIQLETEMNTYLGT 494 HSP 2 Score: 124.405 bits (311), Expect = 4.708e-28 Identity = 65/159 (40.88%), Postives = 98/159 (61.64%), Query Frame = 1
Query: 1891 LYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
L Q + E + Y F+D+LE F+ FGYVS+F+C PLA + +LNNI E+ SDAFK+ ++RPF +IGVWQ+A E +S +AV N L+ +S + + A ++T +VAIEHV+L KF++ IP+VP I+ + ++ + EALK
Sbjct: 479 LEQIQLETEMNTYLGTFDDYLEQFLLFGYVSLFSCVHPLAAVLVVLNNITEVYSDAFKMCHVFKRPFSEPAANIGVWQLAFETMSIIAVVTNCALIALSPQVKAYFPESDAQLILT-VVAIEHVLLAFKFILAFVIPDVPKHIQVKLSKLDFESLEALK 636
BLAST of Anoctamin vs. TrEMBL
Match: A0A4Z2DXS9 (Anoctamin OS=Schistosoma japonicum OX=6182 GN=EWB00_010472 PE=3 SV=1) HSP 1 Score: 585.104 bits (1507), Expect = 0.000e+0 Identity = 333/816 (40.81%), Postives = 483/816 (59.19%), Query Frame = 1
Query: 28 MTILDEAKEHFKNFKRDIQSTAKILIKSSTKWGKXXXXXXXXXXXXLTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKF-GLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVG-QENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQ----VWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKL-DTLEIMKKCQDRSQRKTIHKTISTDSVDEMSEFSGNKRPDFLNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSDCNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRHRVHSCDALEKKGENDN 2454
MTILDE + + ++ + T+K ++ + KW ++S+VPS D D+V+ F S + + W + + + VP+L +HR + +YALYLTASY+ LL GAE L I+K ++ E GG R+F++DEL LF +V E+IFL++ ER +I+ ++L SLRA E S + +Q ++P L S I QIFPLH + L L IW+ ++ QPL+EI YFG+K+ALYFAWLGHYT +L+ P I+GL+ W V +N YYL A+ N++W + YL+HWKRT S Y+W + D I LL E RP FKG +++CP+T R +R YP KR + FV+ PV+ L ++ VI ++L++ Q V D N + +L PKI LA I MD ++KIA+ L ENHRLED Y NH + KLIL QF+NSF+SLFY AFYL+DL LR+QL TLL+IRQ I +I+EI +P +R+RQ + +RYE+++L + ++ + + S + I+ S+ SE N+ D +S +E SE+ + + +V++ D KL +S +S P E + AE EATL +Y+DP +DFLEMFIQFGYVSMF FPLA L +NNI+EIR DA+KL +SYQRPF SIGVWQ+AL+ +SYVAV VN+ LLG+SG R+ S +I +V IEH +++++ I+ +P+ P + + +EH+RREALK +E+E+M+E HR S ++ E+DN
Sbjct: 1 MTILDECHKKLTDLQKQVTQTSKNVLSRTQKWRRLSTVPSTDCDVVVIFKSELSEELVDWLIKIIRKRVPQLVVHKQFHRTSRQYALYLTASYRGLLTGAEELRIKKPLLPEHGGDLREFSVDELSLFDNVMNENIFLSTSERANIIHHFLMSLRACREDSDICSIRFADDQCMIPSLQSAGIILQIFPLHEQDELDKLTNIWIRRWIVLQPLDEIKEYFGIKVALYFAWLGHYTYSLIFPAIVGLVVWLFVNPNKNSSFYYLLMAIVNLIWTSLYLEHWKRTSSFLAYRWSSWDKPISLLEEPRPLFKGTLSLCPVTGRPVRTYPKRKRLLILCFVTTPVVLLSLIIVIYITLMYVRLQEKMNVLADSNTTIIPNF-VLVTLPKIFLALSISLMDLSYRKIAIWLTDFENHRLEDDYNNHYVFKLILLQFVNSFYSLFYTAFYLKDLELLRKQLVTLLLIRQIIDSIREIFLPLGQSRLRQVWLSLRYEQKRLAEGSKLKSESYNSSSPIRTNSAINLSSIQ--SEIDNNQNCD--------------------DSGSKEVDVN-SEMHIHQRVIQTVTN----------------------DDKLGSNSKSSD-------NPEEIHITPAEREATLLRYEDPTDDFLEMFIQFGYVSMFGGIFPLASLLAFMNNIIEIRGDAYKLSTSYQRPFAKFANSIGVWQLALDAVSYVAVIVNIALLGISGTAQRLFPNLSTSQLIICLVFIEHAIIVMRAAISALVPHTPSSVVHQIAKLEHRRREALKILERESMRE--HHRQQSPPNMQ---ESDN 758
BLAST of Anoctamin vs. TrEMBL
Match: A0A5K4FED7 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 581.637 bits (1498), Expect = 0.000e+0 Identity = 325/806 (40.32%), Postives = 481/806 (59.68%), Query Frame = 1
Query: 28 MTILDEAKEHFKNFKRDIQSTAKILIKSSTKWGKXXXXXXXXXXXXLTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRA-RNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVG-QENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQ----VWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLEIMKKCQDRSQRKTIHKTISTDSVDEMSEFSGNKRPDFLNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSDCNISKRHLSKNIK---GESRRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRHRVHS 2418
MTILDE + + ++ + T++ +I + KW ++S+VPS D D+V+ F + + + W + + VP+L +HR + +YALYLTASY LL GAE L I+K ++ E GGG R+F++DE LF +V +E +FL++ ER +IV ++L SLRA R ++ + +Q ++P L S I QIFPLH N L L IW+ ++ QPL+EI YFG K+A YFAWLGHYT +L+ P+++GL W V +N YYL A+ N++W + YL+HWKRT S Y+W + D+ I LL E RP FKG +++CP+T R IR YP WKR + F++ P++ ++ VI ++LI+ Q ++ D N + IL PKI LA I MD +KKIA+ L ENHRLED Y NH + KLIL QF+NSF+SLFY AFYL+DL LR+QL TLL+ RQ I +I+E +P ++R+RQ + ++YE+R L +R++ K+ S + + S + + + D S ++CS +P +D NI++ ++ N + + + + KL+ +S + S +TP AE EATL +Y+DP +DFLEMFIQFGYVSMF FPLAGL +NNI+EIR DA+KL +SYQRPFG SIG+WQ+AL+ +SY+AV VN+ LLG+SG R+ S+ +I +V IEH +++ + I+ +P+ P + + +EH+RREALK +E+E+M+E R + S
Sbjct: 1 MTILDECHKKLADLQKQVTQTSRNVISRTQKWRRLSTVPSTDCDVVVIFKTELSEEIVDWLIKIIKNRVPQLVIHKQFHRTSRQYALYLTASYIGLLTGAEELRIKKSLLPEHGGGLREFSVDEFSLFDNVMDEKLFLSTSERSNIVHHFLMSLRACREDSDMCSIKFDNDQCMIPSLQSAGIILQIFPLHEPNELNKLTSIWIRRWVVLQPLDEIKEYFGTKVAFYFAWLGHYTYSLIFPSVVGLAVWLFVSPNKNSSFYYLLMAIINLIWTSLYLEHWKRTSSFLAYRWSSWDTPISLLEEPRPLFKGILSLCPITGRPIRTYPKWKRLLILCFITTPIVLFSLIIVIYITLIYVQLQEKMNIYADNNTSIIPNF-ILVTLPKIFLAVSISIMDFSYKKIAIWLTDFENHRLEDDYNNHYVVKLILLQFVNSFYSLFYTAFYLKDLELLRKQLVTLLLTRQIIDSIRETFLPLGHSRLRQVWLSLKYEQRCL---------VERNKLKSKDNNGSLSTSNLSSSLAPSSKTD---------------------SDVDKCSISPENNSQ-------CADNNITRMNVDVNPEIFIHQRIQTVTEENKLNYNSKSFDHSEETRITP-------AEREATLLRYEDPTDDFLEMFIQFGYVSMFTGIFPLAGLLAFMNNIIEIRGDAYKLSTSYQRPFGKFANSIGIWQLALDVVSYIAVIVNIALLGISGTAQRLFPNLSSSQLIIFLVLIEHAIIITRAAISALVPHTPTSVVLQIAKLEHRRREALKILERESMREHQRQQSPS 761
BLAST of Anoctamin vs. TrEMBL
Match: K1QYU7 (Anoctamin OS=Crassostrea gigas OX=29159 GN=CGI_10028235 PE=3 SV=1) HSP 1 Score: 544.658 bits (1402), Expect = 3.246e-178 Identity = 321/793 (40.48%), Postives = 460/793 (58.01%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGL----IGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLEIMKKCQDRSQRKTIHKTISTDSVDEMSEFSGNKRPDFLNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSD-CNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKET----------MKERIRHRVHSCDALEKKGENDNAHRDSAVLTRSRSKM 2499
+TF + +D + W + L P L + +H N +LTA+Y+ LLRGAE L I+K + + GGG ++F +E + F ++ +E+ F +SQERQSI+ + L +LRA + ++ QSIVP+L S + IS++FPLH ++ L + WV F +PQPL++I YFGVKIALYFA+LGHYT+AL +P +GL WQ QE D ++ ALFN WAT YL+HWKR E Y WGT+DSQ E+L + RP + G + + +T R YYP+WKR F YF+S PVI +C+L V Q WI+ ++ + S PKILLA IG +D+I+K IA LN EN+R+E++Y NHL+ KL+LFQF+NSF SLFYIAFYL+D+ +L +QLA +LI RQ IGN+KE +P+ + + +G K + + SQ+ TDS SEFS + D ++S S+ E+C+ E S+E V V+D C + +R ++ GE+++ +K++ +K + +LT QAE E+ + +Y+D +D+LEMFIQFGYV++F+ AFPLA LLNN++EIRSDAFKL +YQRPFG V +IG WQ ALE + +A+ VN L+GVSG + RI+ S VI +IV +EH++L +KFLI ++IP++P IE R +E RREALK E E ++E+I R S E N+ SAV + S M
Sbjct: 42 VTFPADTDTTTLMWILDRLRARSPYLVIHIRHHSNTKGSMFHLTATYEGLLRGAEELGIQKLLKEDLGGGMKEFTFEEQECFNNIEDENQFFSSQERQSIIHHLLDNLRAIKGEQLGKVKFVEGQSIVPVLQSNKIISEVFPLHCTQSIEELRKDWVQAFFSPQPLDKIKDYFGVKIALYFAYLGHYTLALCMPAFVGLGIWITQWQ-ADQEWDDELFIGFALFNAFWATLYLEHWKRKSCELAYHWGTLDSQREMLKDPRPLYTGRLEVSSVTGRLEPYYPSWKRNLFRYFISYPVILICLLFV----------QEWINSLVKSGDVPGFFSFLPKILLAVCIGILDEIYKTIAKWLNDWENYRMEETYWNHLIVKLVLFQFVNSFLSLFYIAFYLRDMDRLCDQLAAILITRQIIGNLKEAFLPYFQWKAKLYKVGYEMTKDLITP-------DNPSQQ-------FTDSPS--SEFSKSPGND-------------AELSSPDGSNTEQCNSMDRE--SIE--VDGVTDSCGVRQRGATE---GENQK--------TKENFQTKSTKPLSLT-------QAEVESAMKQYEDTLDDYLEMFIQFGYVTLFSSAFPLAAFCALLNNVVEIRSDAFKLCMTYQRPFGKTVENIGTWQDALELMGVIAIIVNCALIGVSGQVQRIIPGASREMVIIIIVLLEHIILGLKFLIAYAIPDIPETIETNRAKLEFLRREALKKFESEMQSIAPSSPAEIREKILARQKS--RRENLHHNETKQETSAVNSAKSSDM 770
BLAST of Anoctamin vs. TrEMBL
Match: A0A095A0N2 (Anoctamin OS=Schistosoma haematobium OX=6185 GN=MS3_07463 PE=3 SV=1) HSP 1 Score: 536.954 bits (1382), Expect = 3.107e-175 Identity = 325/820 (39.63%), Postives = 476/820 (58.05%), Query Frame = 1
Query: 28 MTILDEAKEHFKNFKRDIQSTAKILIKSSTKWGKXXXXXXXXXXXXLTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRA-RNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVG-QENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDK-NIEEKNWYS-----ILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIA------------FYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLEIMKKCQDRSQRKTIHKTISTDSVDEMSEFSGNKRPDFLNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSDCNISKRHLSKN--IKGESRRNKIPDL-KLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRHRVHS 2418
MTILDE + + ++ + T+K +I + KW ++S+VPS D D+V+ F + + + W + + VP+L +HR + +YALYLTASY+ LL GAE L I+K ++ E GGG R+F++DE LF +V +E +FL++ ER +IV ++L SLRA R ++ + +Q ++P L S I QIFPLH + L L IW+ ++ QPL+EI YFG K+A YFAWLGHYT +L+ P+++GL W V +N YYL A+ N++W + YL+HWKRT S Y+WG+ D+ I LL E RP FKG++++CP+T R IR Y WKR + F++ P++ L ++ VI ++LI+ Q DK NI N S IL PKI LA I MD +KKIAV L ENHRLED Y NH + KLIL F +++A YL +L QL TLL+ RQ I +I+EI +P ++R+RQ + ++YE+R L +R++ K S D+ ++ F+ P + A+ +S ++CS P D NI++ + N I R K+ + KL+ +S + S +TP AE EATL +Y+DP +DFLEMFIQFGYVSMF FPLAGL +NN++EIR DA+KL +SYQRPFG SIG+WQ+AL+ +SY+AV VN+ LLG+SG + R+ SA +I +V IEH +++ + I+ +P+ P + + +EH+RREALK +E+E+M+E R + S
Sbjct: 1 MTILDECHKKLADLQKQVTQTSKNVINRTQKWRRLSTVPSTDCDVVVIFKTELSEEIVDWLIKIINNRVPQLVIHKQFHRTSRQYALYLTASYRGLLTGAEELRIKKSLLPEHGGGLREFSVDEFSLFDNVMDEKLFLSTSERSNIVHHFLMSLRACREDSDVCSIKFANDQCMIPSLQSAGIILQIFPLHEPSELNKLTSIWIRRWVVLQPLDEIKEYFGTKVAFYFAWLGHYTYSLIFPSVVGLAVWLFVNPNKNSSFYYLLMAIINLIWTSLYLEHWKRTSSFLAYRWGSWDTPISLLEEPRPLFKGKLSLCPITGRPIRTYSKWKRLLILCFITTPIVLLSLIIVIYITLIYVRLQ---DKMNIYADNNTSIIPNFILVTLPKIFLAVSISIMDYSYKKIAVWLTDFENHRLEDDYNNHYVVKLILINFR---LVAYHVAPEFTWIPVPPEQGYLY-FTRLILQLVTLLLTRQIIDSIREIFLPLGHSRLRQVWLSLKYEQRCL---------AERNKLK------SEDNNGSLATFN----PSSSSSLSLAQ-------PSKTDSDVDKCSILPENKS-------QCVDNNITRMDVDVNSEILIHQRIQKVTEKNKLNNNSKSFDNSEETRITP-------AEREATLLRYEDPTDDFLEMFIQFGYVSMFTGIFPLAGLLAFMNNVIEIRGDAYKLSTSYQRPFGQFANSIGIWQLALDVVSYIAVIVNIALLGISGTVQRLFPNLSASQLIIFLVLIEHAIIITRAAISALVPHTPTSVVLQIAKLEHRRREALKILERESMREHQRQQSPS 773
BLAST of Anoctamin vs. TrEMBL
Match: A0A3P9P9W1 (Anoctamin OS=Poecilia reticulata OX=8081 PE=3 SV=1) HSP 1 Score: 536.569 bits (1381), Expect = 3.198e-174 Identity = 296/770 (38.44%), Postives = 442/770 (57.40%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTR----------VRQTIMGIRYEKRKLDTLEIM---KKCQDRSQRKTIHKTISTDSVDEMSEFSGNKRPDFLNISENAEFPILEDISKVPESHGEECSF-------------TPSELGSLESAVPSVSDCNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGE----TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEK 2379
+TFA +D + W + + +PEL + +H++ YA ++TA+Y+NLLRGAE + +RK V EFGGG R F+ +E +++++ E F TSQERQ+I+KYW+ +LRA+ + L+ Q I+P L ++ I QIFPLH IL L + WV QPL++I YFGVKIA+YFAWLG YT ++L P +IG + W L Q + DI + ALFN++WAT +L+ WKR +E YKWGT+D+ + L E RP F+G P+T + YYP W+R+ F + VSLP+ LC+ V V LI Q ++ I+E + PKI+LA + D++++KIA LN MEN+RL+ +Y +L+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLIIRQF+ N+KE++ P++ R V ++ + + +L ++ KKC + + + FS + + I + + I KV +E F P+ + + + PS SD S RH RR + + + P +KR S + P E TTL QAE E+ + Y D F+D+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL + QRPFG V SIG WQ A+E + +A+ VN L+G G L R+ S I IV +EH +L+K++I+ +IP++P W+++E ++++RREA K E+
Sbjct: 53 MTFADTTDDHTLLWLLNHIRLGIPELIIQIRHHKHTRVYAFFITATYENLLRGAEEMGLRKGVKPEFGGGARSFSCEEDYIYENIESELCFFTSQERQTIIKYWMDNLRAKQGEVLHNIIFLEGQPIIPELRARGVIQQIFPLHEQRILNQLMKSWVQAVCEKQPLDDICDYFGVKIAMYFAWLGFYTTSMLYPAVIGFVLWMLTESDQTSRDICCVVFALFNVVWATLFLERWKRRGAELAYKWGTLDTPSKSLEEPRPQFRGVKRCSPITGCEEFYYPPWRRRVFRWLVSLPICILCLCFVFLVMLICFELQEFV-MGIKEMP--RLARFIPKIMLAITVSACDEVYRKIACWLNDMENYRLQSAYEKNLIIKMVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLIIRQFLQNVKEVLQPYLYERHKLGELSLRAVWDLLLSVLLKYARLAAGKVQASEKKCLNGGCGVPDEEEGGERDEADSGRFSEGETEEENMIDCGLKLRKVSFIEKVQSGASDELYFLEEGSPTMVEKGMDPASVFEISAPPPSASDGAASLRH--------RRRGQ----SVERTEPKTKRES--WMDPPEEMESTTLTQAEMESCMQTYPDTFQDYQEMFVQFGYVVLFSSAFPLAAMCALINNIIEIRSDAFKLCTGLQRPFGVRVESIGQWQTAMEAMGLIAIIVNCYLIGQCGQLQRLFPWLSPEMAIISIVILEHFAILLKYVIHVAIPDIPTWVKEEMAKLDYQRREAFKVKEE 805
BLAST of Anoctamin vs. Ensembl Cavefish
Match: ano8b (anoctamin 8 [Source:NCBI gene;Acc:103021376]) HSP 1 Score: 382.104 bits (980), Expect = 4.559e-116 Identity = 189/438 (43.15%), Postives = 277/438 (63.24%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR 1473
+TFA D + W + + +PEL + +H++ YA ++TA+Y+NLLRGAE + +RK + EFGGG R F+ +E +++++ E F TSQERQSI+KYWL +LRA+ + L+ Q I+P L ++ I+Q+FPLH IL L + WV QPL++I YFGVKIA+YFAWLG YT ++L P +IG + W L Q + DI + ALFN++WAT +L+ WKR +E YKWGT+D+ E L E RP F+G P+T + YYP W+R+ F + VS PV LC+ V LI Q ++ + K + P+I+LA + D+++KKIA LN MEN+RL+ +Y +L+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLIIRQF+ N+KE++ P++ R R + +R
Sbjct: 83 MTFADNIDDHTLLWLLNHIRLGIPELIIQIRHHKHTRVYAFFVTATYENLLRGAEEMGLRKSIKPEFGGGSRSFSCEEDYIYENIESELCFFTSQERQSIIKYWLDNLRAKPGEVLHNIHFLEGQPIIPELKARGVITQVFPLHEQRILGQLMKSWVQAVCEKQPLDDICDYFGVKIAMYFAWLGFYTTSMLYPAVIGFVLWMLTESDQTSRDICCVVFALFNVVWATLFLERWKRRGAELAYKWGTLDTPAESLEEPRPQFRGVKRCSPVTGCEEFYYPPWRRRLFTWLVSFPVCILCLCFVFLAMLICFELQEFV---MGIKELPRVARFIPEIILAITVTACDEVYKKIACWLNDMENYRLQSAYEKNLIIKMVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLIIRQFLQNLKEVLQPYLYERHRLGELTLR 517 HSP 2 Score: 166.392 bits (420), Expect = 1.285e-41 Identity = 100/245 (40.82%), Postives = 138/245 (56.33%), Query Frame = 1
Query: 1651 EDISKVPESHGEECSFTPSELGSLESAVPSVSDCNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGE---TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
+D + VPE+ E S P GS SA P + RR + L + ++R S PGE TTL QAE E+ + Y+D F+D+ EMFIQFGYV +F+ AFPLA + L+NNI+EIRSDAFKL + QRPFG V SIG WQ +E + +A+ VN L+G G L R+ S I IV +EH +L+K++I+ +IP++P W+ +E +E +RREA K E
Sbjct: 657 DDDNGVPETK-ELTSEPPPPAGSESSAPPRLR-----------------RRGR----SLERADSKTRRESWID-PPGEHVTTTLTQAEIESCMQTYEDTFQDYQEMFIQFGYVVLFSSAFPLAAMCALINNIIEIRSDAFKLCTGLQRPFGMRVESIGQWQTVMEVMGLIAIIVNCYLIGQCGQLQRLFPWLSPEMAIISIVILEHFAILLKYVIHVAIPDIPGWVGEEMAKLEFQRREAFKKHE 878
BLAST of Anoctamin vs. Ensembl Cavefish
Match: ano10a (anoctamin 10 [Source:NCBI gene;Acc:103032363]) HSP 1 Score: 243.817 bits (621), Expect = 2.889e-69 Identity = 155/407 (38.08%), Postives = 236/407 (57.99%), Query Frame = 1
Query: 298 ASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDK--NIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF---------VNTRVRQTIMGIR 1473
+++K LL GAE + + K+ G+ N + FQ + D FL+ E Q I+K+ L +LRA+ E+ G + +K +S++ L SK + Q FPLH+ LK L W K + + QPL++I YFG +ALYF +L ++T AL+ +IG I + L E++D Y LF A+FN++W+T +L+ WKR ++ Y WGT+ S+ + E RP F G + P+T R+ YPN KRQ +Y VS+P + LC+ + V +I+ + W N + W S+L P I+ A VI M+ +++ A L ENHRLE S++NHL+ K+++F F+N F SLFYIAF +QD+V LR+ LATLLI Q + I E +P+ V+ RVR+ ++G R
Sbjct: 74 STWKRLLIGAEDVGLFKEFHDGTMRGFTYANRESFKEFQ--GDGDDFLSIAECQYIIKHELDTLRAKGESHVPGYAKVKLYPGKSVIRRLQSKAILLQYFPLHDKEELKRLSVSWYRKIKLSFQPLDDIRHYFGEGLALYFGFLEYFTFALVPMALIG-IPYYLFAWEDYDKYVLF-AVFNLVWSTVFLEVWKRCSAQLAYGWGTL-SRKKAFEEPRPGFHGALGFNPVTGREEPVYPNTKRQLKIYLVSVPFVILCLYLSLYVMMIYFDMEYWALSIYNEDPNLWTSVLLFIPSIIYAVVIEIMNLLYRYAAEFLTGWENHRLESSFQNHLVLKVLVFNFINCFASLFYIAFVMQDMVLLRQSLATLLITSQILNQIMEAFLPYWLQRRRNKKVHKRVRR-LLGDR 474 HSP 2 Score: 125.561 bits (314), Expect = 2.771e-29 Identity = 62/157 (39.49%), Postives = 95/157 (60.51%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
Q + E + Y F+D+LE F+ FGYVS+F+C +PL+ L +LNN+ E+ SDAFK+ ++RPF IGVWQ+A E +S +AV N L+ +S + + +I +VA+EHV+L KF++ IP+VP I+ + +E + EALK
Sbjct: 481 QVQLEGDMYTYLGTFDDYLEQFLLFGYVSLFSCVYPLSALLVVLNNVTEVYSDAFKMCKVFKRPFSEPASDIGVWQLAFETMSAIAVVTNCALIALSPQVKAYFPE-GETQLILTVVAVEHVILAFKFILAFIIPDVPKHIQIKLAKLEFESLEALK 636
BLAST of Anoctamin vs. Ensembl Cavefish
Match: ano10a (anoctamin 10 [Source:NCBI gene;Acc:103032363]) HSP 1 Score: 243.432 bits (620), Expect = 3.124e-69 Identity = 155/407 (38.08%), Postives = 236/407 (57.99%), Query Frame = 1
Query: 298 ASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDK--NIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF---------VNTRVRQTIMGIR 1473
+++K LL GAE + + K+ G+ N + FQ + D FL+ E Q I+K+ L +LRA+ E+ G + +K +S++ L SK + Q FPLH+ LK L W K + + QPL++I YFG +ALYF +L ++T AL+ +IG I + L E++D Y LF A+FN++W+T +L+ WKR ++ Y WGT+ S+ + E RP F G + P+T R+ YPN KRQ +Y VS+P + LC+ + V +I+ + W N + W S+L P I+ A VI M+ +++ A L ENHRLE S++NHL+ K+++F F+N F SLFYIAF +QD+V LR+ LATLLI Q + I E +P+ V+ RVR+ ++G R
Sbjct: 74 STWKRLLIGAEDVGLFKEFHDGTMRGFTYANRESFKEFQ--GDGDDFLSIAECQYIIKHELDTLRAKGESHVPGYAKVKLYPGKSVIRRLQSKAILLQYFPLHDKEELKRLSVSWYRKIKLSFQPLDDIRHYFGEGLALYFGFLEYFTFALVPMALIG-IPYYLFAWEDYDKYVLF-AVFNLVWSTVFLEVWKRCSAQLAYGWGTL-SRKKAFEEPRPGFHGALGFNPVTGREEPVYPNTKRQLKIYLVSVPFVILCLYLSLYVMMIYFDMEYWALSIYNEDPNLWTSVLLFIPSIIYAVVIEIMNLLYRYAAEFLTGWENHRLESSFQNHLVLKVLVFNFINCFASLFYIAFVMQDMVLLRQSLATLLITSQILNQIMEAFLPYWLQRRRNKKVHKRVRR-LLGDR 474 HSP 2 Score: 125.176 bits (313), Expect = 3.357e-29 Identity = 62/157 (39.49%), Postives = 95/157 (60.51%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
Q + E + Y F+D+LE F+ FGYVS+F+C +PL+ L +LNN+ E+ SDAFK+ ++RPF IGVWQ+A E +S +AV N L+ +S + + +I +VA+EHV+L KF++ IP+VP I+ + +E + EALK
Sbjct: 481 QVQLEGDMYTYLGTFDDYLEQFLLFGYVSLFSCVYPLSALLVVLNNVTEVYSDAFKMCKVFKRPFSEPASDIGVWQLAFETMSAIAVVTNCALIALSPQVKAYFPE-GETQLILTVVAVEHVILAFKFILAFIIPDVPKHIQIKLAKLEFESLEALK 636
BLAST of Anoctamin vs. Ensembl Cavefish
Match: ano10a (anoctamin 10 [Source:NCBI gene;Acc:103032363]) HSP 1 Score: 243.817 bits (621), Expect = 3.488e-69 Identity = 155/407 (38.08%), Postives = 236/407 (57.99%), Query Frame = 1
Query: 298 ASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDK--NIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF---------VNTRVRQTIMGIR 1473
+++K LL GAE + + K+ G+ N + FQ + D FL+ E Q I+K+ L +LRA+ E+ G + +K +S++ L SK + Q FPLH+ LK L W K + + QPL++I YFG +ALYF +L ++T AL+ +IG I + L E++D Y LF A+FN++W+T +L+ WKR ++ Y WGT+ S+ + E RP F G + P+T R+ YPN KRQ +Y VS+P + LC+ + V +I+ + W N + W S+L P I+ A VI M+ +++ A L ENHRLE S++NHL+ K+++F F+N F SLFYIAF +QD+V LR+ LATLLI Q + I E +P+ V+ RVR+ ++G R
Sbjct: 74 STWKRLLIGAEDVGLFKEFHDGTMRGFTYANRESFKEFQ--GDGDDFLSIAECQYIIKHELDTLRAKGESHVPGYAKVKLYPGKSVIRRLQSKAILLQYFPLHDKEELKRLSVSWYRKIKLSFQPLDDIRHYFGEGLALYFGFLEYFTFALVPMALIG-IPYYLFAWEDYDKYVLF-AVFNLVWSTVFLEVWKRCSAQLAYGWGTL-SRKKAFEEPRPGFHGALGFNPVTGREEPVYPNTKRQLKIYLVSVPFVILCLYLSLYVMMIYFDMEYWALSIYNEDPNLWTSVLLFIPSIIYAVVIEIMNLLYRYAAEFLTGWENHRLESSFQNHLVLKVLVFNFINCFASLFYIAFVMQDMVLLRQSLATLLITSQILNQIMEAFLPYWLQRRRNKKVHKRVRR-LLGDR 474 HSP 2 Score: 125.561 bits (314), Expect = 2.958e-29 Identity = 62/157 (39.49%), Postives = 95/157 (60.51%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
Q + E + Y F+D+LE F+ FGYVS+F+C +PL+ L +LNN+ E+ SDAFK+ ++RPF IGVWQ+A E +S +AV N L+ +S + + +I +VA+EHV+L KF++ IP+VP I+ + +E + EALK
Sbjct: 481 QVQLEGDMYTYLGTFDDYLEQFLLFGYVSLFSCVYPLSALLVVLNNVTEVYSDAFKMCKVFKRPFSEPASDIGVWQLAFETMSAIAVVTNCALIALSPQVKAYFPE-GETQLILTVVAVEHVILAFKFILAFIIPDVPKHIQIKLAKLEFESLEALK 636
BLAST of Anoctamin vs. Ensembl Cavefish
Match: ano10a (anoctamin 10 [Source:NCBI gene;Acc:103032363]) HSP 1 Score: 236.884 bits (603), Expect = 1.836e-67 Identity = 145/369 (39.30%), Postives = 219/369 (59.35%), Query Frame = 1
Query: 412 QDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDK--NIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPF---------VNTRVRQTIMGIR 1473
Q+ + D FL+ E Q I+K+ L +LRA+ E+ G + +K +S++ L SK + Q FPLH+ LK L W K + + QPL++I YFG +ALYF +L ++T AL+ +IG I + L E++D Y LF A+FN++W+T +L+ WKR ++ Y WGT+ S+ + E RP F G + P+T R+ YPN KRQ +Y VS+P + LC+ + V +I+ + W N + W S+L P I+ A VI M+ +++ A L ENHRLE S++NHL+ K+++F F+N F SLFYIAF +QD+V LR+ LATLLI Q + I E +P+ V+ RVR+ ++G R
Sbjct: 44 QNGGDGDDFLSIAECQYIIKHELDTLRAKGESHVPGYAKVKLYPGKSVIRRLQSKAILLQYFPLHDKEELKRLSVSWYRKIKLSFQPLDDIRHYFGEGLALYFGFLEYFTFALVPMALIG-IPYYLFAWEDYDKYVLF-AVFNLVWSTVFLEVWKRCSAQLAYGWGTL-SRKKAFEEPRPGFHGALGFNPVTGREEPVYPNTKRQLKIYLVSVPFVILCLYLSLYVMMIYFDMEYWALSIYNEDPNLWTSVLLFIPSIIYAVVIEIMNLLYRYAAEFLTGWENHRLESSFQNHLVLKVLVFNFINCFASLFYIAFVMQDMVLLRQSLATLLITSQILNQIMEAFLPYWLQRRRNKKVHKRVRR-LLGDR 408 HSP 2 Score: 125.176 bits (313), Expect = 1.859e-29 Identity = 62/157 (39.49%), Postives = 95/157 (60.51%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
Q + E + Y F+D+LE F+ FGYVS+F+C +PL+ L +LNN+ E+ SDAFK+ ++RPF IGVWQ+A E +S +AV N L+ +S + + +I +VA+EHV+L KF++ IP+VP I+ + +E + EALK
Sbjct: 415 QVQLEGDMYTYLGTFDDYLEQFLLFGYVSLFSCVYPLSALLVVLNNVTEVYSDAFKMCKVFKRPFSEPASDIGVWQLAFETMSAIAVVTNCALIALSPQVKAYFPE-GETQLILTVVAVEHVILAFKFILAFIIPDVPKHIQIKLAKLEFESLEALK 570
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006831.1 (pep scaffold:Pmarinus_7.0:GL478953:3641:14773:-1 gene:ENSPMAG00000006170.1 transcript:ENSPMAT00000006831.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 265.003 bits (676), Expect = 3.780e-80 Identity = 160/418 (38.28%), Postives = 250/418 (59.81%), Query Frame = 1
Query: 184 SDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDV--SEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPL-NEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSL-IFSSFQVWIDKNIEEKNWYS-ILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIK 1416
+ M + W + L PEL +++ L+LTAS+ +L+RGAE + +RK +F +++ + F S LTSQERQ+I+ + L ++RAR+ ++ L+ Q+I P L++K ++QIFPLH L H + W+ F QPL ++I YFGVK+A+YFAWLG YT A+ P GL+ W Q + DI + +LFN++WAT +L+ WKR +E +++WGT+DS LL E RP F G I P+T + YYP W+R+ F Y VS+P LC+L + V L +F+ F+ + + ++ + + ++ PK+LLA V+ +D+++K+IA LN +EN+RL+ +Y L+ KL+ FQF+NSF SLFYIAFYL D+ +L+EQLA ++ + +K
Sbjct: 3 TPMPTVLWVLDILKAKAPELLVEISHIPCT---VLHLTASHDDLVRGAEAMGLRKDRHCDFQKLRKNWFPKKAANFVHAYNSPNTDPLTSQERQAIICHTLDNVRARSGERVGNVNFLEGQAIFPELLAKGVVAQIFPLHESRALAHFAKTWMYGFLEKQPLVDQICEYFGVKVAVYFAWLGFYTSAMFYPAFFGLLLWLCTTSDQTSQDISCVAFSLFNVVWATLFLEGWKRHSAELSHRWGTLDSLPGLLEEPRPQFCGRRRISPITGLEEFYYPPWRRRMFRYLVSIP---LCLLSLSAVFLAMFACFE--LQEYVKAIDGVTPLMQFLPKLLLALVVSLLDEVYKRIAFWLNHLENYRLQKAYEKQLIIKLVSFQFVNSFSSLFYIAFYLGDMQRLKEQLAAFSLVTEATRKVK 412
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002040.1 (pep scaffold:Pmarinus_7.0:GL477271:1126:24271:1 gene:ENSPMAG00000001835.1 transcript:ENSPMAT00000002040.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 266.544 bits (680), Expect = 4.727e-77 Identity = 154/400 (38.50%), Postives = 238/400 (59.50%), Query Frame = 1
Query: 310 NLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPI----LVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGW------QLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSS----FQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFV-NTRVRQTIM 1464
+LL G ELL+IRK V +EFGGG + F DE D+F++V E +FL+SQERQSI+ WL +LRA + L+ Q IVP L++++ I Q+FPLH+ LK + WV + QPL+E+ YFGVK+A+YFAWLG+YT AL+ P ++GL+ W Q++G E + +F A + + R + + + + S + +GE + +T R+ YYP+WKR+ F VS+PV CV ++L+F++ FQ+ D + + + PK+LLA ++ ++D++ IA LN EN+RL+ +Y HL+ KL++FQF+NS+ SLFYIAFYL+D+ +L+E LA LLI RQ + N++E + P+V R R+ ++
Sbjct: 1 SLLCGVELLSIRKPVRAEFGGGMQPFMRDE-DIFENVDNEALFLSSQERQSIIFTWLQNLRAGPGERLHDVFFLEGQPIVPRAVLELLNRKVIDQVFPLHDRGTLKKFMKSWVRPSFHQQPLDEVCEYFGVKVAMYFAWLGYYTSALVYPAVLGLLLWVYCYTTQVLGNEGVRMRAIFLAPNGNMRGQ--VTRLGRRLATFIHHTSHLLSLSPSPPPRAAAAQGEKRVSNVTGREQLYYPSWKRELFRCLVSVPV------CVAGLALVFATMLACFQLQ-DYLLSVEGVSDCVHYLPKVLLAVIVTLLNDVYNSIAYWLNDRENYRLQTTYEAHLIIKLVMFQFINSYLSLFYIAFYLKDMKRLKEMLAALLITRQLMQNLREALQPYVIRHRPRRPVL 390
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002034.1 (pep scaffold:Pmarinus_7.0:GL477271:1126:24346:1 gene:ENSPMAG00000001835.1 transcript:ENSPMAT00000002034.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 267.7 bits (683), Expect = 5.492e-77 Identity = 160/425 (37.65%), Postives = 244/425 (57.41%), Query Frame = 1
Query: 310 NLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPI----LVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRT-CSE-------YTYKW-----GTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSS----FQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLEIMKKCQ 1521
+LL G ELL+IRK V +EFGGG + F DE D+F++V E +FL+SQERQSI+ WL +LRA + L+ Q IVP L++++ I Q+FPLH+ LK + WV + QPL+E+ YFGVK+A+YFAWLG+YT AL+ P ++GL+ W +Y T + LKHW CS ++ KW ++ S + +GE + +T R+ YYP+WKR+ F VS+PV CV ++L+F++ FQ+ D + + + PK+LLA ++ ++D++ IA LN EN+RL+ +Y HL+ KL++FQF+NS+ SLFYIAFYL+D+ +L+E LA LLI RQ + N++E + P+V ++R+ + +R D + C+
Sbjct: 1 SLLCGVELLSIRKPVRAEFGGGMQPFMRDE-DIFENVDNEALFLSSQERQSIIFTWLQNLRAGPGERLHDVFFLEGQPIVPRAVLELLNRKVIDQVFPLHDRGTLKKFMKSWVRPSFHQQPLDEVCEYFGVKVAMYFAWLGYYTSALVYPAVLGLLLW---------VYCYTTQVLG-----NELKHWLHCPCSRGADEGNFFSAKWKHEGAXSLLSLSPSPPPRAAAAQGEKRVSNVTGREQLYYPSWKRELFRCLVSVPV------CVAGLALVFATMLACFQLQ-DYLLSVEGVSDCVHYLPKVLLAVIVTLLNDVYNSIAYWLNDRENYRLQTTYEAHLIIKLVMFQFINSYLSLFYIAFYLKDMKRLKEMLAALLITRQLMQNLREALQPYVMEKLRRMDLA----RRPRDATHAPEPCE 399 HSP 2 Score: 156.762 bits (395), Expect = 1.678e-39 Identity = 75/161 (46.58%), Postives = 107/161 (66.46%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEK 2379
QAE E+ + +Y D ED+LEM++QFGYV +F+ AFPLA L L NN++EI +DAFKL + QRPFG V +IG+WQ A+E + VA+ VN L+G SG L + +S+ ++ IV +EH LL+K+ I IP+VP W+ +E +E +RRE K E+
Sbjct: 568 QAEMESCMDQYKDTIEDYLEMYVQFGYVVLFSSAFPLAALCALANNLMEIHTDAFKLCTGLQRPFGQRVRNIGLWQDAMELMVSVAIMVNCALIGQSGQLKSLFPSFSSEGIVLAIVLLEHCGLLLKYTILKLIPDVPSWVAEEMAKLEFQRRETFKRYER 728
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Match: ano10a (anoctamin 10a [Source:NCBI gene;Acc:566425]) HSP 1 Score: 229.565 bits (584), Expect = 8.630e-65 Identity = 151/415 (36.39%), Postives = 229/415 (55.18%), Query Frame = 1
Query: 265 RNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDI-FLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERP-SFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWI-------DKNIEEKNWYSILSP-TPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMG 1467
R A L + A++K LL GAE ++ G R F F+ S++++ FLT E+Q IVK+ L +LRA+ E S G S +K +S+ L SK + QI+PLH+ LK L +W + R + QPL I YFG +IALYF +LG +T AL+ ++GL + L E+ D Y +F L N+ WAT L+ WKR +E + WGT+ + + E RP GE+ P+T R+ YP+W+R VY VS+P + C+ V ++ + W+ D + +E ++L+ P ++ A VI M+ ++ +A L + ENHRLE +++NHL+ K+++F F N F SLFYIAFY+QD+ LR+ LA LLI Q + + E I+P+ + R MG
Sbjct: 62 RTARAATLLVGATWKRLLIGAEEAGFVRRCQD---GSMRAFTYLSRHTFEGYSDDNVAFLTMAEQQYIVKHALNNLRAKEEISMPGYSDIKLYPGKSVFRRLQSKRLLVQIYPLHDTEELKKLSALWYQQARPSYQPLEVIRRYFGEEIALYFGFLGFFTWALVPMGLVGL-PYYLFAWEDFDKYVVFAGL-NLAWATVVLEMWKRHSAELAFCWGTLLMRHDF--EVRPXGVHGELGTNPVTGRREPTYPSWRRSLRVYAVSVPFVCACLYLSYLVMDVYFWMEAWVLAWRHSGDGDADEGGPLAVLALYVPSVIYAVVIEVMNRAYRYLAELLTEWENHRLESTHQNHLILKVLVFNFTNCFASLFYIAFYMQDMKLLRQSLAALLITSQILNQLVESILPYWLRKRRNQQMG 469 HSP 2 Score: 134.806 bits (338), Expect = 7.348e-33 Identity = 72/200 (36.00%), Postives = 113/200 (56.50%), Query Frame = 1
Query: 1798 RRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSA--LHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMK 2391
RRN+ S+ +P+ + S Q E + Y F+D+LE+F+QFGYVS+F+CA+PLA + +LNNI E+RSDA KL +RPF +IGVWQ+A E + +AV N L+ +S + Q + L+ + +IVA EH +L +KF++ IP+ P W+ Q+ I+++ +ALK + + K
Sbjct: 463 RRNQQMGWSSSEKAPSDHKDS--------PLRQQIRIEGEMDTYLGTFDDYLELFLQFGYVSLFSCAYPLAAVLAVLNNITEVRSDALKLCCVLKRPFSLPAANIGVWQLAFEVMGLMAVMTNCALIAMSEQVRTFFDQDGSVTLNYVLVIVAAEHALLALKFMLTFVIPDKPRWVRQKLARIDYESLQALKKQKIQVFK 654
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000399.1 (pep scaffold:Pmarinus_7.0:GL477564:2673:43187:-1 gene:ENSPMAG00000000358.1 transcript:ENSPMAT00000000399.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 102.064 bits (253), Expect = 1.849e-22 Identity = 110/388 (28.35%), Postives = 172/388 (44.33%), Query Frame = 1
Query: 385 FNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNH-------NILKHLGEIWVT--KFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIG----LIGW------------QLVGQ------------------ENHDIY---YLF----TALFNI---LWATFYLKHWKRTCSEYTYKW---GTIDSQIELLTEERPSFKGEM------TICPLTRRQIRYYP-NWKRQSFVYFVSLPVIFLCVLC--VIEVSLIFS--SFQVWIDKNIE---EKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAF 1338
F+ D+ LF D+ ED F S R IV L ++ K G++ L ++ + FPLH+ N K L + W + KF QPL I YFG KI LYFAWLG YT L+ P+++G L GW ++GQ EN D +LF T LF+I LWAT +L+HWKR + Y W G +S+ +L RP ++ ++ L + Q Y +W+ Q + ++ I VL I V +IF + V + + + + N +I+ T ++ +I +D+I+ IA L +E + E+ + L+ K + +F+NS+ S+ Y+AF
Sbjct: 185 FSRDKQKLF-DIKSEDSFFDSATRSRIVFEILKRVKCTKGPYKMGITSL---------IANGVYTSAFPLHDGELHGSEVNDRKLLHDEWASYNKFYKYQPLELIKKYFGEKIGLYFAWLGVYTQMLIPPSVVGIAVFLYGWLAAEKDIPGFETCVIGQNITMCPTCDKLCKYKRLSENCDRVRSSHLFDNITTVLFSIFMALWATTFLEHWKRIETRLNYTWDLNGMEESEQQL----RPEYEAKLLEKKRKVDIKLAKTQTEEYGLSWRDQLPSFLINCSCILFMVLLSFAIVVGMIFYRITMNVLLANSTDPYLKSNRSAIIMATAFLINLVIILILDEIYGSIARWLTVIEVPKTEEEFETRLIFKSFMLKFVNSYTSIIYVAF 558 HSP 2 Score: 77.7962 bits (190), Expect = 6.826e-15 Identity = 32/81 (39.51%), Postives = 53/81 (65.43%), Query Frame = 1
Query: 1945 DFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVS 2187
+++EM IQFG+V++F +FPLA F LLNN++EIR D K ++ +RP IG W + L G++ +AV +N ++ ++
Sbjct: 660 EYMEMVIQFGFVTLFVASFPLAPFFALLNNVIEIRLDGKKFVTELRRPTAVRAKDIGTWYVILNGVAKLAVIINALVIALT 740
BLAST of Anoctamin vs. Ensembl Nematostella
Match: EDO38807 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:A7SBX8]) HSP 1 Score: 337.806 bits (865), Expect = 5.757e-104 Identity = 178/432 (41.20%), Postives = 259/432 (59.95%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQ 1455
+TF ++ + W + L VP++ + N+ Y YLTASY+ LR AE L +RKK+I GGG +F +++ +LF+ + F TS +RQS++ + LRA + ++ + IVP L+++ + ++FPLHNH+ L L + WV F QPL++I YFGVKIA+YFAWLG YT L+ P I+G++ + L G+ D L +FNI+WAT YL+ WKR +E Y+WGT+D E L + RP F+GE +T + +YP+ KR F Y VSLP I + V+ L FQ W+D N L PKI LA IG +DD +KK+A KLN EN+RL+++Y NHL+ KL+ FQF N++ SLFYIAFY+QDL +L+ L LLI +Q +GN+KE ++PFV +V++
Sbjct: 56 ITFPKDTEDYVVMWLLSRLRARVPDIEVHVRQMANSPVYGFYLTASYETFLRQAEELGLRKKLI---GGGMVEFKMEDKELFEKHDDPTAFFTSGDRQSMILEMVNGLRAVDGDELGKITFCEGMQIVPRLMAEGVVEKMFPLHNHDDLAKLRKSWVQAFFKKQPLDDICEYFGVKIAMYFAWLGMYTKWLIAPAILGIVTFVLSMRGEVTRDWCVLAFNIFNIVWATLYLEAWKRKSAELAYRWGTLDMPSEALKDPRPLFRGEFKPSEITGQMEPHYPSIKRNIFRYCVSLPAIIGSLGVVVISMLCCFEFQRWVDMM---DNPPKPLKFAPKIALAVCIGMLDDNYKKLAYKLNDKENYRLQETYENHLIIKLVSFQFFNAYLSLFYIAFYIQDLTRLKNTLGALLITKQVVGNVKEALVPFVKQKVKE 481 HSP 2 Score: 182.57 bits (462), Expect = 3.687e-48 Identity = 85/160 (53.12%), Postives = 116/160 (72.50%), Query Frame = 1
Query: 1891 LYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKA 2370
++QAE E+ +P+Y+D FED+LEMFIQFGYV +F+ AFPLA L L NN++EIRSDAFKL ++ +RPFG V +IG WQ A+E + VAV VN+ LLG+ G + R+ + I LIV +EH+VL IKF + ++IP++P W+E E +E KRREALK
Sbjct: 512 MHQAEIESNMPEYEDTFEDYLEMFIQFGYVVLFSSAFPLAALCALANNVIEIRSDAFKLCTNLRRPFGERVENIGTWQDAMEVMGVVAVMVNLALLGMGGSVQRMFPGMTVTERIILIVVLEHLVLGIKFAVAYAIPDIPEWVENEIAKVEFKRREALKG 671
BLAST of Anoctamin vs. Ensembl Nematostella
Match: EDO33029 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:A7STD6]) HSP 1 Score: 258.455 bits (659), Expect = 7.900e-76 Identity = 156/428 (36.45%), Postives = 248/428 (57.94%), Query Frame = 1
Query: 298 ASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEE-DIFLTSQERQSIVKYWLYSLRARNETSKFGLS---ILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKN--WYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLEIMKKCQDRSQRKTIHKTIST 1563
AS + LL GAE L +RKK S G DF L+ F D + + +LT+ E Q +V L +L++ + + G S I K ++IVP LVSK +S I+PLH LK L + W PL++I YFG +A+YFA+L Y++ L IP ++ ++ + L + +F A+ N+LWAT +L+ WKR CSE ++KWGT+ IE+ E RP++ G + I P+T Q +YY KR+ Y +S P++ LC+ V L++ Q ++++ + + ++L P + + +I +++I+ +IA+ L + ENHRLE SY NHL+ KL+LF F+N F+SLFYIAFYLQD+ LR LA L+I Q IG I E ++P++ R R T + +K + + ++ + + Q++ H T+ +
Sbjct: 3 ASTRRLLLGAEKLKMRKKHKS---GAMVDFCLNFSQEFDDFTGDGQGYLTTAECQRVVYSALNALKSYTDITVPGYSGIHIFKNRAIVPKLVSKGVVSSIYPLHEAQKLKLLEKKWFLNVSAKLPLDDIKEYFGETMAMYFAFLQFYSMTL-IPPVLLIVVFALSNAHDQTKNTVF-AVLNLLWATIFLEAWKRRCSEMSFKWGTLKGGIEV-EEPRPNYWGPLRISPITGHQEQYYSPLKRKLKTYGISYPIVLLCMKVATVVMLLYFKLQFYMEEKYGKDDSIIATVLLMIPSVSYSVMIAVLNNIYHRIALWLTEWENHRLESSYNNHLIVKLVLFYFVNCFYSLFYIAFYLQDIALLRTHLAALMITSQVIGQITESLVPYLMFRSRVTTLSKEGKKIVVKSADLTDSIEKQGQQE--HYTVGS 422 HSP 2 Score: 112.464 bits (280), Expect = 1.046e-25 Identity = 50/127 (39.37%), Postives = 80/127 (62.99%), Query Frame = 1
Query: 1984 MFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREAL 2364
+F+ A+P+A + LLNN++EIR+DAFK+ +QRPF SIG WQ A E +S +AV N L+G++ + + S + + + VAIEH +L +KFL+ IP+VP W++ E +++ + AL
Sbjct: 441 LFSSAYPMAAFWALLNNVIEIRTDAFKMCRIFQRPFSQPASSIGAWQAAFEAMSVIAVITNCALIGMAANSAHWLPDLSPANAVLMFVAIEHFLLGVKFLVAMVIPDVPQWVQDEMAKQDYQAKLAL 567
BLAST of Anoctamin vs. Ensembl Nematostella
Match: EDO31129 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:A7SYT2]) HSP 1 Score: 135.961 bits (341), Expect = 2.966e-37 Identity = 59/142 (41.55%), Postives = 92/142 (64.79%), Query Frame = 1
Query: 1939 FEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREAL 2364
F D+LE+F+QFGY +F+ A+P+A + LLNN++EIR+DAFK+ +QRPF SIG WQ A E +S +AV N L+G++ + + S + + + VAIEH +L +KFL+ IP+VP W++ E +++ + AL
Sbjct: 2 FGDYLELFLQFGYTFLFSSAYPMAAFWALLNNVIEIRTDAFKMCRIFQRPFSQPASSIGAWQAAFEAMSVIAVITNCALIGMAANSAHWLPDLSPANAVLMFVAIEHFLLGVKFLVAMVIPDVPQWVQDEMAKQDYQAKLAL 143
BLAST of Anoctamin vs. Ensembl Nematostella
Match: EDO27523 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:A7T910]) HSP 1 Score: 132.494 bits (332), Expect = 3.519e-36 Identity = 63/125 (50.40%), Postives = 86/125 (68.80%), Query Frame = 1
Query: 2002 PLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
PLA L L NN++EIRSDAFKL ++ +RPFG V +IG WQ A+E + VAV VN+ LLG+ G + R+ + I LIV +EH+VL IKF + ++IP++P W+E E +E KRREALK I+
Sbjct: 1 PLAALCALANNVIEIRSDAFKLCTNLRRPFGERVENIGTWQDAMEVMGVVAVMVNLALLGMGGSVQRMFPGMTVTERIILIVVLEHLVLGIKFAVAYAIPDIPEWVENEIAKVEFKRREALKVIQ 125
BLAST of Anoctamin vs. Ensembl Nematostella
Match: EDO27906 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:A7T7Z0]) HSP 1 Score: 108.612 bits (270), Expect = 1.019e-26 Identity = 53/133 (39.85%), Postives = 85/133 (63.91%), Query Frame = 1
Query: 1141 SILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLEIMKKCQDRSQRK 1539
++L P + + +I +++I+ +IA+ L + ENHRLE SY NHL+ KL+LF F+N F+SLFYIAFYLQD+ LR LA L+I Q IG I E ++P++ R R T + +K + + ++ + + Q++
Sbjct: 24 TVLLMIPSVSYSVMIAVLNNIYHRIALWLTEWENHRLESSYNNHLIVKLVLFYFVNCFYSLFYIAFYLQDIALLRTHLAALMITSQVIGQITESLVPYLMFRSRVTTLSKEGKKIVVKSADLTDSIEKQGQQE 156 HSP 2 Score: 92.0485 bits (227), Expect = 6.042e-21 Identity = 47/136 (34.56%), Postives = 76/136 (55.88%), Query Frame = 1
Query: 1741 VSDCNISKRHL-----SKNIKGESRRNKIPDLKL-SKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMA 2130
+ D + + HL + + G+ + +P L S+ + SK + + T E++ Y D F D+LE+F+QFGY +F+ A+P+A + LLNN++EIR+DAFK+ +QRPF V SIG WQ++
Sbjct: 91 LQDIALLRTHLAALMITSQVIGQITESLVPYLMFRSRVTTLSKEGKKIVVKSADLTD-SIEKQGQQEHYTDTFGDYLELFLQFGYTFLFSSAYPMAAFWALLNNVIEIRTDAFKMCRIFQRPFSQPVSSIGAWQVS 225
BLAST of Anoctamin vs. Ensembl Medaka
Match: ano8a (anoctamin-8 [Source:NCBI gene;Acc:101163031]) HSP 1 Score: 494.967 bits (1273), Expect = 1.350e-159 Identity = 289/796 (36.31%), Postives = 438/796 (55.03%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQL--VGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTL------EIMKKCQDRSQRKTIHKTISTDSVDEMSEFS-----------GNKRPDFLNISE-NAEFPILE---DISKVPESHGEECSFT-------------PSELGSLESAVPSVSDCNISKRHLSKNIKGESRRNKIPDLK-LSKDSPN----SKRSSVC------------------ALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIE 2376
+TF D + W + ++ +P++S + + A ++T +++NLLRGAE + + K V ++FGGG + F+ +E ++++++ E F TSQERQSI+KYWL +LRA++ + L+ Q I+P LV++ I Q+FPLH IL HL WV QPL++I YFGVKI +YFAWLG YT ++L P +IG + W L Q + D+ + ALFN++WAT +L+ WKR +E ++WGT+D+ E L E RP F+G P+T + YYP WKR F + VSLPV LC+ V L+ Q + +E I PKILLA + D+++KKIA LN MEN+RL+ +Y N+L+ K++ F+F+NS+ SLFYI FYL+D+ +L+E LATLLI RQF+ NIKE++ P++ YE+ KL E+++ + R + K ++ M+ +S N P+ + A F + E D+S H + SFT P++ SLE + P++ D + + + +S D + LS+D+P+ + SVC P + L QAE E+ + Y+ +D+ EMFIQFGYV +F+ AFPLA + L+NNI+EIRSD KL + QRPFG V +IG WQ A+E + +A+ VN L+G G L R+ S I IV +EH +L+K++I+ +IP++P W+ E +E++RREA K E
Sbjct: 41 MTFPETIDDHTLLWLLNQVRVGIPQVSIQVRQLGHTQAQAFFITTTFENLLRGAEQMGMHKAVKAQFGGGMQRFSCEEDNIYENIESELCFFTSQERQSIIKYWLDNLRAKHGEVLHNIHFLEGQPIIPELVARGVIQQMFPLHEQRILNHLMTSWVQAVCEKQPLDDICDYFGVKIGMYFAWLGFYTNSMLYPAVIGFLLWILAEADQTSQDVCCVVFALFNVVWATLFLERWKRREAELAFRWGTLDTPAESLEEPRPQFRGVKRCSPITGCEEFYYPPWKRAVFRWLVSLPVCVLCLCFVFLAMLLCLELQEVV---MEIPELPGITRFIPKILLAVTVTICDEVYKKIAYWLNNMENYRLQSAYENNLIIKMVFFEFINSYLSLFYIGFYLKDMERLKEMLATLLIFRQFLQNIKEVLQPYL------------YEQNKLGVFTPKVLWELLQAIMLKYGRLALGKAQAS-----MTSYSFLGTRLSVNAAANGNPELRRRGDLKAGFRLTEEEWDMSDSAMKH-RKVSFTEKVHYQDVTATARPADESSLEDS-PTMVDEGMDPSSIFDSCDEDS------DCELLSRDNPSRPPPKETDSVCHRRRNSEAKDDKKSWIDPPEEPKKVGLTQAEIESCMQTYEGTLQDYQEMFIQFGYVILFSSAFPLAAMCALINNIVEIRSDGLKLCTGLQRPFGQRVENIGQWQTAMEAMGLIAIIVNCYLIGQCGQLQRLFPWLSPEMTIVSIVLLEHFAILLKYIIHVAIPDIPGWVADEMAKLEYRRREAFKKHE 808
BLAST of Anoctamin vs. Ensembl Medaka
Match: ano8b (anoctamin 8 [Source:NCBI gene;Acc:101168196]) HSP 1 Score: 384.03 bits (985), Expect = 3.992e-119 Identity = 188/438 (42.92%), Postives = 281/438 (64.16%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR 1473
LTFA +D + W + + +PEL + +H++ YA ++TA+Y+NLLRGAE + +RK V EFGGG R+F+ +E +++++ E F TSQERQSI+K+WL +LRA++ + ++ L Q I+P L ++ I Q+FPLH IL HL + WV QPL++I YFGVKIA+YFAWLG YT ++L P +IG + W L Q + DI + ALFN++WAT +L+ WKR +E YKWGT+D+ E L E RP F+G P+T + YYP W+R F + VSLP+ LC+ V +I Q ++ + K ++ PKILLA + D++++KIA LN MEN+RL+ +Y +L+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLIIRQF+ ++E++ P++ R + + +R
Sbjct: 44 LTFADTTDDHTLLWLLNHIRLGIPELIIQIRHHKHTRVYAFFITATYENLLRGAEEMGLRKAVKPEFGGGTRNFSCEEDYIYENIESELCFFTSQERQSIIKHWLDNLRAKHGEALHNITFLGGQPIIPELRARGVIQQVFPLHEQRILSHLMKSWVQAVCEKQPLDDICDYFGVKIAMYFAWLGFYTTSMLYPAVIGFVLWMLTESDQTSRDICCVVFALFNVVWATLFLERWKRRSAELAYKWGTLDAPSESLEEPRPQFRGVKRCSPITGCEEFYYPPWRRLVFRWLVSLPICILCLCFVFLAMIICFELQEFV---MGIKEMPRLVRFIPKILLAITVTACDEVYRKIACWLNDMENYRLQSAYEKNLIIKMVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLIIRQFLQTVREVLQPYLYERHKLGELSLR 478 HSP 2 Score: 169.088 bits (427), Expect = 1.020e-42 Identity = 79/161 (49.07%), Postives = 110/161 (68.32%), Query Frame = 1
Query: 1885 TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALK 2367
TTL QAE E+ + Y D FED+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL + QRPFG V IG WQ A+E + +A+ VN L+G G L R+ S I IV +EH +L+K++I+ +IP+VP W+++E ++++RREA K
Sbjct: 663 TTLTQAEMESCMQTYADTFEDYQEMFVQFGYVVLFSSAFPLAAMCALINNIIEIRSDAFKLCTGLQRPFGVRVACIGQWQTAMEAMGLIAIIVNCYLIGQCGQLQRLFPWLSPEMAIVSIVILEHFAVLLKYIIHVAIPDVPTWVKEEMAKLDYQRREAFK 823
BLAST of Anoctamin vs. Ensembl Medaka
Match: ano8b (anoctamin 8 [Source:NCBI gene;Acc:101168196]) HSP 1 Score: 385.571 bits (989), Expect = 1.416e-117 Identity = 188/438 (42.92%), Postives = 281/438 (64.16%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR 1473
LTFA +D + W + + +PEL + +H++ YA ++TA+Y+NLLRGAE + +RK V EFGGG R+F+ +E +++++ E F TSQERQSI+K+WL +LRA++ + ++ L Q I+P L ++ I Q+FPLH IL HL + WV QPL++I YFGVKIA+YFAWLG YT ++L P +IG + W L Q + DI + ALFN++WAT +L+ WKR +E YKWGT+D+ E L E RP F+G P+T + YYP W+R F + VSLP+ LC+ V +I Q ++ + K ++ PKILLA + D++++KIA LN MEN+RL+ +Y +L+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLIIRQF+ ++E++ P++ R + + +R
Sbjct: 56 LTFADTTDDHTLLWLLNHIRLGIPELIIQIRHHKHTRVYAFFITATYENLLRGAEEMGLRKAVKPEFGGGTRNFSCEEDYIYENIESELCFFTSQERQSIIKHWLDNLRAKHGEALHNITFLGGQPIIPELRARGVIQQVFPLHEQRILSHLMKSWVQAVCEKQPLDDICDYFGVKIAMYFAWLGFYTTSMLYPAVIGFVLWMLTESDQTSRDICCVVFALFNVVWATLFLERWKRRSAELAYKWGTLDAPSESLEEPRPQFRGVKRCSPITGCEEFYYPPWRRLVFRWLVSLPICILCLCFVFLAMIICFELQEFV---MGIKEMPRLVRFIPKILLAITVTACDEVYRKIACWLNDMENYRLQSAYEKNLIIKMVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLIIRQFLQTVREVLQPYLYERHKLGELSLR 490 HSP 2 Score: 169.474 bits (428), Expect = 1.104e-42 Identity = 80/165 (48.48%), Postives = 112/165 (67.88%), Query Frame = 1
Query: 1885 TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEK 2379
TTL QAE E+ + Y D FED+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL + QRPFG V IG WQ A+E + +A+ VN L+G G L R+ S I IV +EH +L+K++I+ +IP+VP W+++E ++++RREA K E+
Sbjct: 706 TTLTQAEMESCMQTYADTFEDYQEMFVQFGYVVLFSSAFPLAAMCALINNIIEIRSDAFKLCTGLQRPFGVRVACIGQWQTAMEAMGLIAIIVNCYLIGQCGQLQRLFPWLSPEMAIVSIVILEHFAVLLKYIIHVAIPDVPTWVKEEMAKLDYQRREAFKKHER 870
BLAST of Anoctamin vs. Ensembl Medaka
Match: ano8b (anoctamin 8 [Source:NCBI gene;Acc:101168196]) HSP 1 Score: 385.956 bits (990), Expect = 2.567e-117 Identity = 188/438 (42.92%), Postives = 281/438 (64.16%), Query Frame = 1
Query: 166 LTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLV--GQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIR 1473
LTFA +D + W + + +PEL + +H++ YA ++TA+Y+NLLRGAE + +RK V EFGGG R+F+ +E +++++ E F TSQERQSI+K+WL +LRA++ + ++ L Q I+P L ++ I Q+FPLH IL HL + WV QPL++I YFGVKIA+YFAWLG YT ++L P +IG + W L Q + DI + ALFN++WAT +L+ WKR +E YKWGT+D+ E L E RP F+G P+T + YYP W+R F + VSLP+ LC+ V +I Q ++ + K ++ PKILLA + D++++KIA LN MEN+RL+ +Y +L+ K++LFQF+NS+ SLFYI FYL+D+ +L+E LATLLIIRQF+ ++E++ P++ R + + +R
Sbjct: 92 LTFADTTDDHTLLWLLNHIRLGIPELIIQIRHHKHTRVYAFFITATYENLLRGAEEMGLRKAVKPEFGGGTRNFSCEEDYIYENIESELCFFTSQERQSIIKHWLDNLRAKHGEALHNITFLGGQPIIPELRARGVIQQVFPLHEQRILSHLMKSWVQAVCEKQPLDDICDYFGVKIAMYFAWLGFYTTSMLYPAVIGFVLWMLTESDQTSRDICCVVFALFNVVWATLFLERWKRRSAELAYKWGTLDAPSESLEEPRPQFRGVKRCSPITGCEEFYYPPWRRLVFRWLVSLPICILCLCFVFLAMIICFELQEFV---MGIKEMPRLVRFIPKILLAITVTACDEVYRKIACWLNDMENYRLQSAYEKNLIIKMVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLIIRQFLQTVREVLQPYLYERHKLGELSLR 526 HSP 2 Score: 169.859 bits (429), Expect = 1.126e-42 Identity = 80/165 (48.48%), Postives = 112/165 (67.88%), Query Frame = 1
Query: 1885 TTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEK 2379
TTL QAE E+ + Y D FED+ EMF+QFGYV +F+ AFPLA + L+NNI+EIRSDAFKL + QRPFG V IG WQ A+E + +A+ VN L+G G L R+ S I IV +EH +L+K++I+ +IP+VP W+++E ++++RREA K E+
Sbjct: 742 TTLTQAEMESCMQTYADTFEDYQEMFVQFGYVVLFSSAFPLAAMCALINNIIEIRSDAFKLCTGLQRPFGVRVACIGQWQTAMEAMGLIAIIVNCYLIGQCGQLQRLFPWLSPEMAIVSIVILEHFAVLLKYIIHVAIPDVPTWVKEEMAKLDYQRREAFKKHER 906
BLAST of Anoctamin vs. Ensembl Medaka
Match: ano10a (anoctamin 10 [Source:NCBI gene;Acc:101169062]) HSP 1 Score: 233.417 bits (594), Expect = 1.755e-65 Identity = 152/404 (37.62%), Postives = 226/404 (55.94%), Query Frame = 1
Query: 298 ASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEE-DIFLTSQERQSIVKYWLYSLRARNETSKFGLSILK---EQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFR-NPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKN--WYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRK 1488
AS++ LL GAE + K+ G R F F+ + D FL+ E Q I+K+ L +LRA++ET G S K +SI+ L SK + Q+FPLH+ LK L W R + QPL+ I YFG ALYF +L ++TIALL ++G + + L EN+D Y LF A+FN++W T L+ WKR + Y WGT+ S+ + E RP F G + + P+T R+ +P+ RQ +Y VSLP + LC+ + V +I+ + W +E W +L+ P I+ A VI ++ +++ A L + ENHR E SY+NHL+ K+++F F N F SLFYIAF +QD++ LR+ LATLLI Q + + E +P+ R R M + +R+
Sbjct: 72 ASWQRLLCGAEDCGLFKEYSD---GSMRGFTCANQHRFKGFTGAGDDFLSMAECQYIIKHQLEALRAKDETHVPGYSQAKLYPGKSIIRRLQSKGILVQMFPLHDKEELKRLSFSWYRTVRLSLQPLDAIRRYFGEGQALYFGFLEYFTIALLPMAVLG-VPYYLFDWENYDKYVLF-AVFNLVWCTVLLEVWKRYSATLAYCWGTL-SRKKAFEEPRPGFHGVLGLNPVTGRKEPVFPSVSRQLRIYLVSLPFVLLCLYLSLYVMMIYFQLEGWALSVYDEDPTLWTEVLTYIPSIVYAVVIEAVNLLYRYAAEFLTEWENHRRESSYQNHLVLKVLVFNFFNCFASLFYIAFVMQDMLLLRQSLATLLITSQVLNQLMEAFLPYWLQRRRNKKMIRKVLRRR 469 HSP 2 Score: 107.842 bits (268), Expect = 1.164e-23 Identity = 53/120 (44.17%), Postives = 76/120 (63.33%), Query Frame = 1
Query: 1897 QAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIE 2256
Q EA + Y F+D+LE+F+ FGYVS+F+C +PLA + +LNNI E+ SDAFK+ ++RPF SIGVWQ+A E +S +AV N L+G+S + + SA +I VA+E
Sbjct: 481 QVRLEADMSTYLGTFDDYLELFLLFGYVSLFSCIYPLAAVLVVLNNITEVYSDAFKMCKLFKRPFADPAGSIGVWQLAFETISVIAVVTNCALIGMSPQVKAYFPE-SATQLILWTVAVE 599
BLAST of Anoctamin vs. Planmine SMEST
Match: SMESG000065607.1 (SMESG000065607.1) HSP 1 Score: 1675.22 bits (4337), Expect = 0.000e+0 Identity = 826/828 (99.76%), Postives = 827/828 (99.88%), Query Frame = 1
Query: 28 MTILDEAKEHFKNFKRDIQSTAKILIKSSTKWGKXXXXXXXXXXXXLTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLEIMKKCQDRSQRKTIHKTISTDSVDEMSEFSGNKRPDFLNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSDCNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRHRVHSCDALEKKGENDNAHRDSAVLTRSRSKMSQDI 2511
MTILDEAKEHFKNFKRDIQSTAKILIKSSTKWGKISSVPSVDVDIVLTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLEIMKKCQDRSQRKTI KTISTDSVDEMSEFSGNKRPDFLNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVS+CNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRHRVHSCDALEKKGENDNAHRDSAVLTRSRSKMSQDI
Sbjct: 1 MTILDEAKEHFKNFKRDIQSTAKILIKSSTKWGKISSVPSVDVDIVLTFASGSDMDFIQWFMLELVENVPELSFSLNYHRNADEYALYLTASYKNLLRGAELLNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNHNILKHLGEIWVTKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIGWQLVGQENHDIYYLFTALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFYLQDLVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGIRYEKRKLDTLEIMKKCQDRSQRKTILKTISTDSVDEMSEFSGNKRPDFLNISENAEFPILEDISKVPESHGEECSFTPSELGSLESAVPSVSECNISKRHLSKNIKGESRRNKIPDLKLSKDSPNSKRSSVCALTPGETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRHRVHSCDALEKKGENDNAHRDSAVLTRSRSKMSQDI 828
BLAST of Anoctamin vs. Planmine SMEST
Match: SMESG000052528.1 (SMESG000052528.1) HSP 1 Score: 106.301 bits (264), Expect = 4.300e-23 Identity = 112/471 (23.78%), Postives = 204/471 (43.31%), Query Frame = 1
Query: 334 LNIRKKVISEFGGGYRDFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYS-LRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNH------------NILKHLGEIWV--TKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLI-----------------------GWQLVGQENHDI----YYL-------------------FTALFNILWATFYLKHWKRTCSEYTYKWGTID--SQIELLTEERPSFKGEMTICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWID----KNIEEKNWYSILSPTPKILLAFV-IGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFY----------------LQD--------LVKLREQLATLLIIRQFIGNIKEIIMPFVNTRVRQTIMGI 1470
LNI ++ ++ F ++ NLD + ++ F T+ +RQ + L + + + ++ G+ L E+ I S +PLH+ N + L E W + + QPL+ I Y+G KI LYFAWLG YT+ LL +IIG++ +++ N DI +YL F ++F WA +L++WKR + Y W I + + E + + + P+T ++P R++F + + VI + + ++ + ++V I +N ++ I + T + + I + +++K+A KL + E HR + + +HL+ K+ LFQF+N + S+FYIAF+ L++ L +L +QLA +++ +Q I N +EI +P + T Q G+
Sbjct: 186 LNIPRRPMAYFTCNFKKSNLDR---YIGCKNQETFFTATQRQQVAYEILATQVYGKRRKAEIGIDRLLEEGIY---------SAAYPLHDGPFQQCGKNADSLNERQMLYENWAKWSMWYKYQPLDHIREYYGEKIGLYFAWLGMYTVWLLPASIIGILVFIYGLLTINKNVPSNEICQSNQTYRMCPLCNEDIGCPYWYLSDVCFYSKITYMFDHPGTVFFSIFMAFWAVSFLENWKRKNASLAYHWDVIHFEEEEQRPRPEYAALCPSVKMNPVTGLMEPHFPT-MRKAFRFLTGICVISIMFVLILIFIIAVIIYRVLIAIPLFQNPLLRSKAIIFANTSAAFVNLILIMCLGRVYEKLAYKLTQWEMHRTQTEFDDHLILKVYLFQFVNFYSSIFYIAFFKGRFNGYPGNYSLFFGLRNEECSNGSCLFELAQQLAIIMVGKQIINNFQEIFVPKILTWFYQCSKGL 643 HSP 2 Score: 84.7297 bits (208), Expect = 2.153e-16 Identity = 60/217 (27.65%), Postives = 100/217 (46.08%), Query Frame = 1
Query: 1882 ETTLYQAEEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVS------------------------------GGLSR------IMKQYSALHVI---------TLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKER 2397
E + AE+ LP Y+ FE++LEM +QFG++++F AFPLA LF LLNN +EIR DA KL+ +RP IG W + L+ + +++V N L+ + G L+ + +Y + +I ++ EHVV + LI+ IP++ Y ++ + ++AL +K+T K +
Sbjct: 654 ELIKFWAEDYKLLP-YEGLFEEYLEMVLQFGFITIFVAAFPLAPLFALLNNWVEIRLDAKKLVCETRRPISERAQDIGKWSVILDAIVHLSVISNAFLIAFTSEFLPKLIYKYRYDDQLRGYVNFTLSWAPNGSLTEPCRYRALRDEYGSYTIIYWQLLALRFGFVIIFEHVVFSVAHLIDIVIPDISYNLQVRMRRENYLAKQALLEFKKKTDKRK 869
BLAST of Anoctamin vs. Planmine SMEST
Match: SMESG000006381.1 (SMESG000006381.1) HSP 1 Score: 103.99 bits (258), Expect = 1.854e-22 Identity = 111/454 (24.45%), Postives = 191/454 (42.07%), Query Frame = 1
Query: 382 DFNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYS-LRARNETSKFGLSILKEQSIV---------PILVSKEKISQIFPLHNHNILKHLGEIWV--TKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGL-------------IGWQLVGQENHDIY--------------------------YLFT-------ALFNILWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMTIC------PLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLCVI-------EVSLIFSSFQVWID-----KNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFY---------------------LQD---LVKLREQLATLLIIRQFIGNIKEIIMPFVNT 1443
+F ++ F + + + +S +R +V L + L R + G+ + + I P SK ++ Q L++ IL E W ++ QPL+ I YFG KI YFAW+G YT LL +IIG+ I + + N+ Y YLF ++F +WA +L++WKR + + W +D + E EERP + +C P+T Y+P +R LP I ++C++ + ++V I+ + N + + T I+ +I + +++ IA+KL + E HR + + N L+ K+ LFQF+N + S+FYIAF+ Q+ L++L QLA +++ +Q I NI+E+I+P + T
Sbjct: 155 EFKQSKIHRFLGSNNKLTYFSSTQRHQVVNEILETQLYGRRRKGEIGIKRMVDDEIYDSAYPLHEGPYECSKTELEQPEKLNSRQILY---EYWARYGRWYQYQPLDHIREYFGEKIGFYFAWMGLYTAWLLPASIIGIFVFLYGVFTFDRNISAKEICAGNNSFYMCPLCDEKIGCQYWELKDVCMFIKIAYLFDHPGTIFYSIFMAMWAVTFLEYWKRKNASLAHHWDVMDYEEE---EERPRAQ-YAALCSIFQKNPVTGVIEPYFPKERR--------LPRILTGIVCILLMMTVVIIFIIAVIIYRVLIEIPMFQNPLLRNNAVTFANGTAAIVNLVLIMCLAKVYEIIALKLTEWEMHRTQSDFDNQLIFKVFLFQFVNFYSSIFYIAFFKGRFVGYPGNYSYFFGLRNEDCQNGGCLIELAMQLAIIMVGKQIISNIQEVIVPKLQT 593 HSP 2 Score: 80.1073 bits (196), Expect = 4.656e-15 Identity = 39/95 (41.05%), Postives = 58/95 (61.05%), Query Frame = 1
Query: 1903 EEEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVS 2187
+E+ L Y+ +++LE+ +QFG++++F AFPLA F LLNN +EIR DA KL+ QRP IGVW + LE ++ +A N LL +
Sbjct: 620 KEDNKLIPYEGLLDEYLEIVLQFGFITIFVAAFPLAPFFALLNNWMEIRLDAQKLVCETQRPVAERAKDIGVWFVILESITTIACISNAFLLAFT 714
BLAST of Anoctamin vs. Planmine SMEST
Match: SMESG000053932.1 (SMESG000053932.1) HSP 1 Score: 102.449 bits (254), Expect = 5.688e-22 Identity = 110/445 (24.72%), Postives = 192/445 (43.15%), Query Frame = 1
Query: 385 FNLDELDLFQDVSEEDIFLTSQERQSIVKYWLYSLRARNETSKFGLSILKEQSIVPILVSKEKISQIFPLHNH---------------NILKHLGEIWV--TKFRNPQPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGL--------------IGWQLVGQEN-------------------------HDIYYLF----TALFNI---LWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERP-----SFKGEMTICPLTRRQIRYYP-NWKRQSFVYFVSLPVIFLCVLCVIEVSLIFSSFQVWI---DKNIEEKNWYSILSPTPKILLAFVIGFMDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAFY----------------LQD--------LVKLREQLATLLIIRQFIGNIKEIIMP 1431
F D+L+ + + ED F ++ +R + + L T+ +G E I ++ +E S +PLH N + L E W +KF QP+ I Y+G KIA+YFAWLG YT L +++G+ I ++ N + YLF T F+I +WA +L++WKR +E +Y+W T+D + E +E+P + E + P+T + Y+P N + + + V V+ ++ + + +I + I D + S + I+ +I + +++K+A KL + E HR E + +HL K+ LF+F+N + S+FYIAF+ L++ L +L +Q+A +++ Q I NI+EI +P
Sbjct: 162 FKTDQLEKYLGYTNEDTFFSTTQRHQVAREIL-------ATTVYGRQTKGEIGI-DRMIDEEIYSAAYPLHEGPAEVPTDLLGKVDLWNRRQILFEYWAKWSKFYKYQPIENIREYYGEKIAIYFAWLGLYTAWLFPASLVGIFVFVYGILTMKSYTISKEICDTNNTFKMCPLCNYEKGCRYWNLSDICFYSQMTYLFDHPGTVFFSIFMSVWAVAFLENWKRKNAELSYRWDTVDYEEE---DEKPRPEYVALATEKRVNPVTGIEEPYFPVNLRMKRMLTGVMCIVVMFMIVIIFIIGVIIYRILIAIPLFDNPLLRSKALIFASVSAAIVNLILIMSLGLVYEKLAYKLTQWEMHRTETEFEDHLTVKVYLFEFVNFYSSIFYIAFFKGRFNGYPGKYSLFFGLRNEDCSNGSCLFELAQQMAIIMVGNQIINNIQEIFLP 595 HSP 2 Score: 83.1889 bits (204), Expect = 6.250e-16 Identity = 62/234 (26.50%), Postives = 105/234 (44.87%), Query Frame = 1
Query: 1906 EEATLPKYDDPFEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLG----------------------------------------------VSGGLSRIMKQYSALHVITLIVAIEHVVLLIKFLINHSIPNVPYWIEQERILIEHKRREALKAIEKETMKERIRHRVHSCDALEKKGENDNAHRDS 2469
E+ L Y+ F+++LEM +QFG++++F AFPLA F L+NN LEIR DA KL+ S +RP +G W + L + +++ N L+ V+G L+R + A+ +T ++ EH+V + +LI+ IP+VP E + IE +R+ + K+ + ++ + S K+GE+ DS
Sbjct: 626 EDYKLLPYEGLFDEYLEMIVQFGFITIFVAAFPLAPFFALINNWLEIRLDASKLVLSTRRPVAERAQDMGTWFIILNLIVKLSIISNAFLIAFTSDFIPKQVYKYSHNFLLDGYVNFTLAWAPNNSLSEPCRYRDFRDVNGKLTRFYWRLLAIR-LTFVIVFEHIVFGVAYLIDILIPDVP-----ESLQIEMRRKNYMA---KQALLNMTKNEMGSL----KRGESLLVKADS 846
BLAST of Anoctamin vs. Planmine SMEST
Match: SMESG000033728.1 (SMESG000033728.1) HSP 1 Score: 90.8929 bits (224), Expect = 2.373e-18 Identity = 103/409 (25.18%), Postives = 177/409 (43.28%), Query Frame = 1
Query: 499 NETSKFGLSILKEQSIV--PILVSKEKISQIFPLHN--HNILKHLGEIWVTKFRNP---QPLNEIASYFGVKIALYFAWLGHYTIALLIPTIIGLIG--WQLVGQENH------------------------------DIYYLF----------TALFNI---LWATFYLKHWKRTCSEYTYKWGTIDSQIELLTEERPSFKGEMT------ICPLTRRQIRYYPNWKRQSFVYFVSLPVIFLCVLC-------VIEVSLI-FSSFQVWIDKNIEEKNWYSILSPTPKILLAFVIGF-MDDIFKKIAVKLNKMENHRLEDSYRNHLMTKLILFQFMNSFFSLFYIAF---YLQDLVKLRE--------------------QLATLLIIRQFIGNIKEIIMPFVNTRVRQ 1455
N+T FG++ L + + ++ + +++ L N +N L + WV+ RN QPL+ I YFG K+A YFAWLG+YT LL +++G+I + +V N D Y F T L + +WA+ +L+ WKR + Y W D + E RP F + ++ ++ + P W+R+ V F + + L + +I L+ FS ++ + I N SI++ LL+ VI + ++ ++AV L ME+HR + Y + + KL L +F+N++ S+FYI F + Q + R QLA ++I +Q I +IKE +P + + V++
Sbjct: 267 NKTVDFGIAKLISEGVFDSAYILHERPLNEDDDLENVENNERNLLYKHWVS-MRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYED-EPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTIS--NMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKR 671 HSP 2 Score: 76.2554 bits (186), Expect = 7.429e-14 Identity = 35/83 (42.17%), Postives = 51/83 (61.45%), Query Frame = 1
Query: 1939 FEDFLEMFIQFGYVSMFACAFPLAGLFCLLNNILEIRSDAFKLISSYQRPFGANVPSIGVWQMALEGLSYVAVAVNMGLLGVS 2187
F ++LEM IQFG++++F AFPLA L L NN+ EIRSDA K + +RP V IG+W + ++ +AV N L+ +
Sbjct: 737 FGEYLEMLIQFGFITIFIPAFPLAPLLALTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFA 819 The following BLAST results are available for this feature:
BLAST of Anoctamin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 4
BLAST of Anoctamin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Anoctamin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Anoctamin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Anoctamin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Anoctamin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Anoctamin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Anoctamin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035175 ID=SMED30035175|Name=Anoctamin|organism=Schmidtea mediterranea sexual|type=transcript|length=2742bpback to top protein sequence of SMED30035175-orf-1 >SMED30035175-orf-1 ID=SMED30035175-orf-1|Name=SMED30035175-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=829bp MTILDEAKEHFKNFKRDIQSTAKILIKSSTKWGKISSVPSVDVDIVLTFAback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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