SMED30035133

Overview
NameSMED30035133
Smed IDSMED30035133
Length (bp)647
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30035133 (SMED30035133) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30035133 (SMED30035133) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30035133 (SMED30035133) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30035133 (SMED30035133) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30035133

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
oocyteSMED30035133 Contig46012newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30035133 Contig46012uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30035133 Contig46012newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30035133 Contig46012uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30035133 vs. Planmine SMEST
Match: SMESG000063795.1 (SMESG000063795.1)

HSP 1 Score: 337.806 bits (865), Expect = 4.041e-118
Identity = 163/195 (83.59%), Postives = 182/195 (93.33%), Query Frame = 2 
Query:   14 RVEMKEGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGAILNVNPKTVQTWMNKPEFNNSHSQFIDRDVETKETISFNKLPKYLTDNNRVEMKEGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGAILNVNPKTVQTWMNKPEFNNSHSQFIDRDVETKETISFNKLPKYLTDNSQVEMN 598
            R  +K+ KENLISN+  KKYNKVSNSAK+EA QMRKNGMTFV IGA+ +VNPKTVQTWMNKPEFN+SHSQFIDRDVETKETISFNKLPKYLTDN++VEMK+GKENLISNNR KKYNKVSNSAKQEA QMR+NGMTFV I  +LNVNPKTV+TWMNKPEFNNSHSQFIDRD+ETK+TISFNKLPKYLT+N+++EMN
Sbjct:   51 RYWIKKDKENLISNSCLKKYNKVSNSAKREAVQMRKNGMTFVAIGAMFDVNPKTVQTWMNKPEFNSSHSQFIDRDVETKETISFNKLPKYLTDNSQVEMKQGKENLISNNRPKKYNKVSNSAKQEAVQMRENGMTFVAIAIMLNVNPKTVKTWMNKPEFNNSHSQFIDRDLETKQTISFNKLPKYLTENNRIEMN 245

HSP 2 Score: 186.808 bits (473), Expect = 8.075e-59
Identity = 88/103 (85.44%), Postives = 98/103 (95.15%), Query Frame = 2 
Query:    2 TDNNRVEMKEGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGAILNVNPKTVQTWMNKPEFNNSHSQFIDRDVETKETISFNKLPKYLTDNNRVEM 310
            TDN++VEMK+GKENLISNNR KKYNKVSNSAKQEA QMR+NGMTFV I  +LNVNPKTV+TWMNKPEFNNSHSQFIDRD+ETK+TISFNKLPKYLT+NNR+EM
Sbjct:  142 TDNSQVEMKQGKENLISNNRPKKYNKVSNSAKQEAVQMRENGMTFVAIAIMLNVNPKTVKTWMNKPEFNNSHSQFIDRDLETKQTISFNKLPKYLTENNRIEM 244
[Close]
BLAST of SMED30035133 vs. Planmine SMEST
Match: SMESG000069874.1 (SMESG000069874.1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.495e-10
Identity = 28/60 (46.67%), Postives = 43/60 (71.67%), Query Frame = 2 
Query:   23 MKEGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGAILNVNPKTVQTWMNKPE 202
            +K+GKE L++N+ +KKY KVS   K++A +M K+GM F  +G I +V+P TV+ WM K +
Sbjct:   44 IKKGKEALLNNSGNKKYIKVSAVLKKQAIEMIKDGMNFEEVGKIFDVHPATVKFWMTKEQ 103

HSP 2 Score: 59.3066 bits (142), Expect = 2.495e-10
Identity = 28/60 (46.67%), Postives = 43/60 (71.67%), Query Frame = 2 
Query:  308 MKEGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGAILNVNPKTVQTWMNKPE 487
            +K+GKE L++N+ +KKY KVS   K++A +M K+GM F  +G I +V+P TV+ WM K +
Sbjct:   44 IKKGKEALLNNSGNKKYIKVSAVLKKQAIEMIKDGMNFEEVGKIFDVHPATVKFWMTKEQ 103
[Close]
BLAST of SMED30035133 vs. Planmine SMEST
Match: SMESG000069876.1 (SMESG000069876.1)

HSP 1 Score: 58.9214 bits (141), Expect = 4.030e-10
Identity = 28/60 (46.67%), Postives = 43/60 (71.67%), Query Frame = 2 
Query:   23 MKEGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGAILNVNPKTVQTWMNKPE 202
            +K+GKE L++N+ +KKY KVS   K++A +M K+GM F  +G I +V+P TV+ WM K +
Sbjct:   44 IKKGKEALLNNSGNKKYIKVSAVLKKQAIEMIKDGMNFEEVGKIFDVHPATVKFWMTKEQ 103

HSP 2 Score: 58.9214 bits (141), Expect = 4.030e-10
Identity = 28/60 (46.67%), Postives = 43/60 (71.67%), Query Frame = 2 
Query:  308 MKEGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGAILNVNPKTVQTWMNKPE 487
            +K+GKE L++N+ +KKY KVS   K++A +M K+GM F  +G I +V+P TV+ WM K +
Sbjct:   44 IKKGKEALLNNSGNKKYIKVSAVLKKQAIEMIKDGMNFEEVGKIFDVHPATVKFWMTKEQ 103
[Close]
BLAST of SMED30035133 vs. Planmine SMEST
Match: SMESG000067278.1 (SMESG000067278.1)

HSP 1 Score: 44.669 bits (104), Expect = 6.779e-6
Identity = 20/63 (31.75%), Postives = 38/63 (60.32%), Query Frame = 2 
Query:   29 EGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGAILNVNPKTVQTWMNKPEFNNSH 217
            +G+++ ++    K+Y   +   K++  +M K+G TF  I  + NV PK V++W+NK + NN +
Sbjct:   49 KGEDHYLTQVTPKQYKSFTKGMKEQTLEMIKSGKTFEEIIKLFNVRPKAVRSWINKNQLNNYY 111

HSP 2 Score: 44.669 bits (104), Expect = 6.779e-6
Identity = 20/63 (31.75%), Postives = 38/63 (60.32%), Query Frame = 2 
Query:  314 EGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGAILNVNPKTVQTWMNKPEFNNSH 502
            +G+++ ++    K+Y   +   K++  +M K+G TF  I  + NV PK V++W+NK + NN +
Sbjct:   49 KGEDHYLTQVTPKQYKSFTKGMKEQTLEMIKSGKTFEEIIKLFNVRPKAVRSWINKNQLNNYY 111
The following BLAST results are available for this feature:
BLAST of SMED30035133 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30035133 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000063795.14.041e-11883.59SMESG000063795.1[more]
SMESG000069874.12.495e-1046.67SMESG000069874.1[more]
SMESG000069876.14.030e-1046.67SMESG000069876.1[more]
SMESG000067278.16.779e-631.75SMESG000067278.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035133 ID=SMED30035133|Name=SMED30035133|organism=Schmidtea mediterranea sexual|type=transcript|length=647bp
GACAGATAATAATCGAGTGGAAATGAAAGAAGGAAAAGAAAATCTCATTT
CAAACAATCGCTCAAAGAAATATAATAAAGTTTCTAATTCAGCAAAACAA
GAGGCCTTTCAAATGAGAAAGAACGGGATGACATTTGTAGTAATTGGTGC
AATATTAAATGTCAATCCGAAAACCGTGCAAACATGGATGAATAAACCGG
AATTTAATAATTCACATTCACAGTTCATTGACAGAGATGTGGAAACTAAA
GAAACAATCTCATTCAATAAGCTACCGAAATATTTGACAGATAATAATCG
AGTGGAAATGAAAGAAGGAAAAGAAAATCTCATTTCAAACAATCGCTCAA
AGAAATATAATAAAGTTTCTAATTCAGCAAAACAAGAGGCCTTTCAAATG
AGAAAGAACGGGATGACATTTGTAGTAATTGGTGCAATATTAAATGTCAA
TCCGAAAACCGTGCAAACATGGATGAATAAACCGGAATTTAATAATTCAC
ATTCACAGTTCATTGACAGAGATGTGGAAACTAAAGAAACAATCTCATTC
AATAAGCTACCGAAATATTTAACAGATAATAGTCAAGTGGAAATGAATTC
GGTGAGTGAAGAAATCTCAGAGATTGAATTCACTGATGAAGAAATCG
back to top

protein sequence of SMED30035133-orf-1

>SMED30035133-orf-1 ID=SMED30035133-orf-1|Name=SMED30035133-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=215bp
TDNNRVEMKEGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGA
ILNVNPKTVQTWMNKPEFNNSHSQFIDRDVETKETISFNKLPKYLTDNNR
VEMKEGKENLISNNRSKKYNKVSNSAKQEAFQMRKNGMTFVVIGAILNVN
PKTVQTWMNKPEFNNSHSQFIDRDVETKETISFNKLPKYLTDNSQVEMNS
VSEEISEIEFTDEEI
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000231vitelline gland