slc43a-1

Overview
Nameslc43a-1 (AKN21683.1)
Smed IDSMED30035121
Length (bp)1746
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of slc43a-1 (SMED30035121) t-SNE clustered cells

Violin plots show distribution of expression levels for slc43a-1 (SMED30035121) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of slc43a-1 (SMED30035121) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for slc43a-1 (SMED30035121) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30035121

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30035121h1SMcG0020303 SmedASXL_007180SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of slc43a-1 vs. Ensembl Human
Match: SLC43A3 (solute carrier family 43 member 3 [Source:HGNC Symbol;Acc:HGNC:17466])

HSP 1 Score: 55.0694 bits (131), Expect = 2.536e-7
Identity = 32/114 (28.07%), Postives = 62/114 (54.39%), Query Frame = 1
Query: 1006 SVVISPIVGLMIDYQKNRVS-PSKDQEAFYKSILKCFGIPML-ICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
             V+ +P  GL++D  K +    ++   +   ++  C  +P L + SL  L  ++   +P     Y+ ++   ++R F++  NA+++  AFP ++FGKLFGL + +   VS LQ+
Sbjct:  328 GVLCAPWNGLLMDRLKQKYQKEARKTGSSTLAVALCSTVPSLALTSLLCLGFALCASVPILPLQYLTFILQVISRSFLYGSNAAFLTLAFPSEHFGKLFGLVMALSAVVSLLQF 441          
BLAST of slc43a-1 vs. Ensembl Human
Match: SLC43A3 (solute carrier family 43 member 3 [Source:HGNC Symbol;Acc:HGNC:17466])

HSP 1 Score: 55.0694 bits (131), Expect = 2.536e-7
Identity = 32/114 (28.07%), Postives = 62/114 (54.39%), Query Frame = 1
Query: 1006 SVVISPIVGLMIDYQKNRVS-PSKDQEAFYKSILKCFGIPML-ICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
             V+ +P  GL++D  K +    ++   +   ++  C  +P L + SL  L  ++   +P     Y+ ++   ++R F++  NA+++  AFP ++FGKLFGL + +   VS LQ+
Sbjct:  328 GVLCAPWNGLLMDRLKQKYQKEARKTGSSTLAVALCSTVPSLALTSLLCLGFALCASVPILPLQYLTFILQVISRSFLYGSNAAFLTLAFPSEHFGKLFGLVMALSAVVSLLQF 441          
BLAST of slc43a-1 vs. Ensembl Human
Match: SLC43A3 (solute carrier family 43 member 3 [Source:HGNC Symbol;Acc:HGNC:17466])

HSP 1 Score: 55.0694 bits (131), Expect = 2.536e-7
Identity = 32/114 (28.07%), Postives = 62/114 (54.39%), Query Frame = 1
Query: 1006 SVVISPIVGLMIDYQKNRVS-PSKDQEAFYKSILKCFGIPML-ICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
             V+ +P  GL++D  K +    ++   +   ++  C  +P L + SL  L  ++   +P     Y+ ++   ++R F++  NA+++  AFP ++FGKLFGL + +   VS LQ+
Sbjct:  328 GVLCAPWNGLLMDRLKQKYQKEARKTGSSTLAVALCSTVPSLALTSLLCLGFALCASVPILPLQYLTFILQVISRSFLYGSNAAFLTLAFPSEHFGKLFGLVMALSAVVSLLQF 441          
BLAST of slc43a-1 vs. Ensembl Human
Match: SLC43A3 (solute carrier family 43 member 3 [Source:HGNC Symbol;Acc:HGNC:17466])

HSP 1 Score: 55.0694 bits (131), Expect = 2.536e-7
Identity = 32/114 (28.07%), Postives = 62/114 (54.39%), Query Frame = 1
Query: 1006 SVVISPIVGLMIDYQKNRVS-PSKDQEAFYKSILKCFGIPML-ICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
             V+ +P  GL++D  K +    ++   +   ++  C  +P L + SL  L  ++   +P     Y+ ++   ++R F++  NA+++  AFP ++FGKLFGL + +   VS LQ+
Sbjct:  328 GVLCAPWNGLLMDRLKQKYQKEARKTGSSTLAVALCSTVPSLALTSLLCLGFALCASVPILPLQYLTFILQVISRSFLYGSNAAFLTLAFPSEHFGKLFGLVMALSAVVSLLQF 441          
BLAST of slc43a-1 vs. Ensembl Human
Match: SLC43A3 (solute carrier family 43 member 3 [Source:HGNC Symbol;Acc:HGNC:17466])

HSP 1 Score: 54.6842 bits (130), Expect = 3.023e-7
Identity = 32/114 (28.07%), Postives = 62/114 (54.39%), Query Frame = 1
Query: 1006 SVVISPIVGLMIDYQKNRVS-PSKDQEAFYKSILKCFGIPML-ICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
             V+ +P  GL++D  K +    ++   +   ++  C  +P L + SL  L  ++   +P     Y+ ++   ++R F++  NA+++  AFP ++FGKLFGL + +   VS LQ+
Sbjct:  341 GVLCAPWNGLLMDRLKQKYQKEARKTGSSTLAVALCSTVPSLALTSLLCLGFALCASVPILPLQYLTFILQVISRSFLYGSNAAFLTLAFPSEHFGKLFGLVMALSAVVSLLQF 454          
BLAST of slc43a-1 vs. Ensembl Zebrafish
Match: slc43a3b (solute carrier family 43 member 3b [Source:ZFIN;Acc:ZDB-GENE-060312-28])

HSP 1 Score: 85.8853 bits (211), Expect = 2.662e-17
Identity = 99/433 (22.86%), Postives = 184/433 (42.49%), Query Frame = 1
Query:  100 FVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATS--TKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRI-----KAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPR----------MKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKN--RVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
            F LI   +E + ++G +FG  SL+ +LK  G+F +    T  N  A    ++Q+E F  I        +  + P G + DR+G    +L+  S    G LL +       IL+FPA   + +      + N  +  L    ++ II++++G+F SS+ +   +   ++ G+ +    L +     I +   R F+L     I     +     +   G  D  I   +  + + TS  ++E       P+           K+   + +FL+ L ++ +   R   F+  L+  +    + D +          +  +  ++ +P  GL++D  K   R     +QEA  K+ +    +  L C L     S+    P     Y+ ++   + R F++  NA++I  AFP  +FGKL+G  + +   VS LQY
Sbjct:   15 FTLISGLVECLGFAGAVFGWASLVFVLKNEGYFSSLCVNTTINGTAVEDCSRQDEQFSLIFTIASFMNNFLTLPNGFLFDRFGTTVARLLGISVYSTGMLLIAFSTTASSILLFPALSFISVGGILFLMTNIQVGNLFGTHRSTIITLYNGAFDSSSAVFLIIKLLFEDGVSLRGCFLFMACCSVIHLF--RTFVLMPRTHIPYPLPENYTYGINCGGTQDYTIAKVEEKN-IQTSQAEEEVAFTESSPQGQNTNKMERSFKSCFLSWFFLWHLLWLSVMQLRHYLFIGTLNPMLNHLTAGDPSLVSRYTNAFAFTQLCGILCAPWNGLLMDRHKGKQREPGQSEQEADLKAAILSLSLTALQCVL----FSVCAATPILPLQYLTFILQVLNRSFLYGGNAAFISVAFPSVHFGKLYGTVMALSAVVSLLQY 440          
BLAST of slc43a-1 vs. Ensembl Zebrafish
Match: slc43a3b (solute carrier family 43 member 3b [Source:ZFIN;Acc:ZDB-GENE-060312-28])

HSP 1 Score: 85.8853 bits (211), Expect = 2.662e-17
Identity = 99/433 (22.86%), Postives = 184/433 (42.49%), Query Frame = 1
Query:  100 FVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATS--TKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRI-----KAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPR----------MKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKN--RVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
            F LI   +E + ++G +FG  SL+ +LK  G+F +    T  N  A    ++Q+E F  I        +  + P G + DR+G    +L+  S    G LL +       IL+FPA   + +      + N  +  L    ++ II++++G+F SS+ +   +   ++ G+ +    L +     I +   R F+L     I     +     +   G  D  I   +  + + TS  ++E       P+           K+   + +FL+ L ++ +   R   F+  L+  +    + D +          +  +  ++ +P  GL++D  K   R     +QEA  K+ +    +  L C L     S+    P     Y+ ++   + R F++  NA++I  AFP  +FGKL+G  + +   VS LQY
Sbjct:   15 FTLISGLVECLGFAGAVFGWASLVFVLKNEGYFSSLCVNTTINGTAVEDCSRQDEQFSLIFTIASFMNNFLTLPNGFLFDRFGTTVARLLGISVYSTGMLLIAFSTTASSILLFPALSFISVGGILFLMTNIQVGNLFGTHRSTIITLYNGAFDSSSAVFLIIKLLFEDGVSLRGCFLFMACCSVIHLF--RTFVLMPRTHIPYPLPENYTYGINCGGTQDYTIAKVEEKN-IQTSQAEEEVAFTESSPQGQNTNKMERSFKSCFLSWFFLWHLLWLSVMQLRHYLFIGTLNPMLNHLTAGDPSLVSRYTNAFAFTQLCGILCAPWNGLLMDRHKGKQREPGQSEQEADLKAAILSLSLTALQCVL----FSVCAATPILPLQYLTFILQVLNRSFLYGGNAAFISVAFPSVHFGKLYGTVMALSAVVSLLQY 440          
BLAST of slc43a-1 vs. Ensembl Zebrafish
Match: slc43a3a (solute carrier family 43 member 3a [Source:ZFIN;Acc:ZDB-GENE-110208-8])

HSP 1 Score: 85.5001 bits (210), Expect = 3.582e-17
Identity = 99/442 (22.40%), Postives = 196/442 (44.34%), Query Frame = 1
Query:  103 VLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTK---QNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDK------------------------VDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNR-VSPS-KDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
             L    +E + ++G++FG  SL+ +LK +G+F +  +   D     S+    Q+E F  +        +  + P G + D +G   T+L++      G L+ +       +L+FPA   + +      + N  +  L    ++ II++++G+F SS+++   +   Y++G+ + +S L +     I +I    FL+ K                        V+  +AK         S +R R  + +     SI   +N +   F   ++ + + +FL+ L ++ +   R  FF+  L+  + +  + +            +     V+ +P  GL++D  K + + P   ++EA  +S +    +  L C L     SI   IP     Y+ ++   + R F++  NA++I  AFP ++FGKL+GL +++   VS LQY
Sbjct:   17 TLATGLLECLCFAGVVFGWASLVFVLKTDGYFGDLCSNATDAGGEFSSDCGLQDEHFSLVFTVASFMNNFLTLPNGFLYDHFGTMATRLLAIFLYTTGTLIVAFSNKALSVLLFPALSFIAVGGILFLMTNMQVGNLFGSHRSTIITVYNGAFDSSSVIFLIIKVLYEKGVSLRSSFLFLSACNLIHLI-RTLFLMPKSHIPYPIPQDYQYGISCAKSNSYNVEEYEAKR------EASSDRSRRAEEELLETDSIKLTDNPEKDSF---RSCVLSWFFLWHLVWLSVMQLRHYFFIGTLNPMLNRLANQNPVTVSEYTNAFAFTQFCGVLCAPWNGLLMDRHKRKPLDPDVSEREADLRSSILSLLLTSLQCFL----FSICASIPFLPLQYLTFILQVLNRSFLYGGNAAFISIAFPARHFGKLYGLVMSLSAVVSLLQY 444          
BLAST of slc43a-1 vs. Ensembl Zebrafish
Match: slc43a3a (solute carrier family 43 member 3a [Source:ZFIN;Acc:ZDB-GENE-110208-8])

HSP 1 Score: 85.5001 bits (210), Expect = 3.582e-17
Identity = 99/442 (22.40%), Postives = 196/442 (44.34%), Query Frame = 1
Query:  103 VLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTK---QNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDK------------------------VDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNR-VSPS-KDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
             L    +E + ++G++FG  SL+ +LK +G+F +  +   D     S+    Q+E F  +        +  + P G + D +G   T+L++      G L+ +       +L+FPA   + +      + N  +  L    ++ II++++G+F SS+++   +   Y++G+ + +S L +     I +I    FL+ K                        V+  +AK         S +R R  + +     SI   +N +   F   ++ + + +FL+ L ++ +   R  FF+  L+  + +  + +            +     V+ +P  GL++D  K + + P   ++EA  +S +    +  L C L     SI   IP     Y+ ++   + R F++  NA++I  AFP ++FGKL+GL +++   VS LQY
Sbjct:   17 TLATGLLECLCFAGVVFGWASLVFVLKTDGYFGDLCSNATDAGGEFSSDCGLQDEHFSLVFTVASFMNNFLTLPNGFLYDHFGTMATRLLAIFLYTTGTLIVAFSNKALSVLLFPALSFIAVGGILFLMTNMQVGNLFGSHRSTIITVYNGAFDSSSVIFLIIKVLYEKGVSLRSSFLFLSACNLIHLI-RTLFLMPKSHIPYPIPQDYQYGISCAKSNSYNVEEYEAKR------EASSDRSRRAEEELLETDSIKLTDNPEKDSF---RSCVLSWFFLWHLVWLSVMQLRHYFFIGTLNPMLNRLANQNPVTVSEYTNAFAFTQFCGVLCAPWNGLLMDRHKRKPLDPDVSEREADLRSSILSLLLTSLQCFL----FSICASIPFLPLQYLTFILQVLNRSFLYGGNAAFISIAFPARHFGKLYGLVMSLSAVVSLLQY 444          
BLAST of slc43a-1 vs. Ensembl Xenopus
Match: slc43a2 (solute carrier family 43 (amino acid system L transporter), member 2 [Source:NCBI gene;Acc:100124757])

HSP 1 Score: 50.8322 bits (120), Expect = 6.125e-6
Identity = 31/108 (28.70%), Postives = 48/108 (44.44%), Query Frame = 1
Query:   94 RCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFF----HNQFNRTYDNANATST---------------KQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKL 360
            R ++   A +E +F+S ++ G  SLL +LK  GF+    H   N TY N+    T               +Q+E        G       S PLG++MD+YG +  +L
Sbjct:   12 RWWMACTAVVENLFFSAVLLGWGSLLIMLKSEGFYSYLCHYPDNTTYHNSTGNETNQELVMDMNGWLICKEQDEMLNLAFTVGSFLLSAISLPLGIIMDKYGPRKLRL 119          
BLAST of slc43a-1 vs. Ensembl Xenopus
Match: slc43a2 (solute carrier family 43 (amino acid system L transporter), member 2 [Source:NCBI gene;Acc:100124757])

HSP 1 Score: 50.447 bits (119), Expect = 6.637e-6
Identity = 31/108 (28.70%), Postives = 48/108 (44.44%), Query Frame = 1
Query:   94 RCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFF----HNQFNRTYDNANATST---------------KQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKL 360
            R ++   A +E +F+S ++ G  SLL +LK  GF+    H   N TY N+    T               +Q+E        G       S PLG++MD+YG +  +L
Sbjct:   12 RWWMACTAVVENLFFSAVLLGWGSLLIMLKSEGFYSYLCHYPDNTTYHNSTGNETNQELVMDMNGWLICKEQDEMLNLAFTVGSFLLSAISLPLGIIMDKYGPRKLRL 119          
BLAST of slc43a-1 vs. Ensembl Mouse
Match: Slc43a3 (solute carrier family 43, member 3 [Source:MGI Symbol;Acc:MGI:1931054])

HSP 1 Score: 65.0846 bits (157), Expect = 1.431e-10
Identity = 103/461 (22.34%), Postives = 193/461 (41.87%), Query Frame = 1
Query:   79 PKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRT---YDNANATS--TKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISG-------FILM----------------ICSR--------KFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDD--ETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVS-PSKDQEAFYKSILKCFGIPMLICSLANL-SMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
            P YL     L+   +E I ++G++FG  SLL + K   +F     +      N    S    Q+E F  I        +  +FP G + DR+     +LI+        ++ +       +L+F A  +L +      I N  +  L  K ++ II++++G+F SS+ +   +   Y++GI + +S + + +         F+LM                +CSR        K    +   +++KE L     E ++     +          +QE ++   F R    L   + L  ++  +I  + +  F+  L+  + K    D  E  A +       FF   V+ +P  GL++D  K +    +K   +  +++  C  +P L  +       ++   IP     Y  ++   V+R F++  NA+++  AFP ++FGKLFGL + +   VS LQ+
Sbjct:    7 PLYLA---TLLTGLLECIGFAGVLFGWTSLLFVFKAENYFSEPCEQDCLLQSNVTGPSDLKAQDEKFSLIFTLASFMNNFMTFPTGYIFDRFKTTVARLIAIFFYTCATIIIAFTSANTAMLLFLAMPMLAVGGILFLITNLQIGNLFGKHRSTIITLYNGAFDSSSAVFLVIKLLYEQGISLRSSFIFMSVCSVWHIARTFLLMPKGHIPYPLPPNYSYGLCSRFGASKKENKAAEHETKELRSKECLPP--KEENSGPEQQQQQEQQQQQQQQQEQHEQHSFRRCA--LSRRFILHVVWLSIIQLWHY-LFIGTLNSLLTKLSGGDKVEVSAYTNAFAITQFF--GVLCAPWNGLLMDRLKQKYQKAAKRTGSSSEAVALCSMVPSLALTSLLSLGFALCASIPVMQLQYATFILQVVSRSFLYGCNAAFLTLAFPSEHFGKLFGLVMALSAIVSLLQF 457          
BLAST of slc43a-1 vs. Ensembl Mouse
Match: Slc43a1 (solute carrier family 43, member 1 [Source:MGI Symbol;Acc:MGI:1931352])

HSP 1 Score: 54.6842 bits (130), Expect = 1.642e-8
Identity = 35/111 (31.53%), Postives = 56/111 (50.45%), Query Frame = 1
Query:   73 LKPKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFF------HNQFNRTYDNANA-TSTKQNE-------TFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLI 363
            LK  Y  R ++   A +E +F+S ++ G  SLL +LK+ GF+       N+ N T D  +  TS  Q E       T G  L++        + PLG++MDR+G +  +L+
Sbjct:    5 LKQAYRRRWWMACTAVVENLFFSAVLLGWASLLIMLKKEGFYSSLCPAENRTNTTQDEQHQWTSCDQQEKMLNLGFTIGSFLLSAT------TLPLGILMDRFGPRPLRLV 109          
BLAST of slc43a-1 vs. Ensembl Mouse
Match: Slc43a1 (solute carrier family 43, member 1 [Source:MGI Symbol;Acc:MGI:1931352])

HSP 1 Score: 52.7582 bits (125), Expect = 1.041e-6
Identity = 35/111 (31.53%), Postives = 56/111 (50.45%), Query Frame = 1
Query:   73 LKPKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFF------HNQFNRTYDNANA-TSTKQNE-------TFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLI 363
            LK  Y  R ++   A +E +F+S ++ G  SLL +LK+ GF+       N+ N T D  +  TS  Q E       T G  L++        + PLG++MDR+G +  +L+
Sbjct:    5 LKQAYRRRWWMACTAVVENLFFSAVLLGWASLLIMLKKEGFYSSLCPAENRTNTTQDEQHQWTSCDQQEKMLNLGFTIGSFLLSAT------TLPLGILMDRFGPRPLRLV 109          
BLAST of slc43a-1 vs. Ensembl Mouse
Match: Slc43a1 (solute carrier family 43, member 1 [Source:MGI Symbol;Acc:MGI:1931352])

HSP 1 Score: 52.7582 bits (125), Expect = 1.041e-6
Identity = 35/111 (31.53%), Postives = 56/111 (50.45%), Query Frame = 1
Query:   73 LKPKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFF------HNQFNRTYDNANA-TSTKQNE-------TFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLI 363
            LK  Y  R ++   A +E +F+S ++ G  SLL +LK+ GF+       N+ N T D  +  TS  Q E       T G  L++        + PLG++MDR+G +  +L+
Sbjct:    5 LKQAYRRRWWMACTAVVENLFFSAVLLGWASLLIMLKKEGFYSSLCPAENRTNTTQDEQHQWTSCDQQEKMLNLGFTIGSFLLSAT------TLPLGILMDRFGPRPLRLV 109          
BLAST of slc43a-1 vs. Ensembl Mouse
Match: Slc43a1 (solute carrier family 43, member 1 [Source:MGI Symbol;Acc:MGI:1931352])

HSP 1 Score: 52.7582 bits (125), Expect = 1.084e-6
Identity = 35/111 (31.53%), Postives = 56/111 (50.45%), Query Frame = 1
Query:   73 LKPKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFF------HNQFNRTYDNANA-TSTKQNE-------TFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLI 363
            LK  Y  R ++   A +E +F+S ++ G  SLL +LK+ GF+       N+ N T D  +  TS  Q E       T G  L++        + PLG++MDR+G +  +L+
Sbjct:   31 LKQAYRRRWWMACTAVVENLFFSAVLLGWASLLIMLKKEGFYSSLCPAENRTNTTQDEQHQWTSCDQQEKMLNLGFTIGSFLLSAT------TLPLGILMDRFGPRPLRLV 135          
BLAST of slc43a-1 vs. UniProt/SwissProt
Match: sp|A2AVZ9|S43A3_MOUSE (Solute carrier family 43 member 3 OS=Mus musculus OX=10090 GN=Slc43a3 PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.001e-9
Identity = 103/461 (22.34%), Postives = 193/461 (41.87%), Query Frame = 1
Query:   79 PKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRT---YDNANATS--TKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISG-------FILM----------------ICSR--------KFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDD--ETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVS-PSKDQEAFYKSILKCFGIPMLICSLANL-SMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
            P YL     L+   +E I ++G++FG  SLL + K   +F     +      N    S    Q+E F  I        +  +FP G + DR+     +LI+        ++ +       +L+F A  +L +      I N  +  L  K ++ II++++G+F SS+ +   +   Y++GI + +S + + +         F+LM                +CSR        K    +   +++KE L     E ++     +          +QE ++   F R    L   + L  ++  +I  + +  F+  L+  + K    D  E  A +       FF   V+ +P  GL++D  K +    +K   +  +++  C  +P L  +       ++   IP     Y  ++   V+R F++  NA+++  AFP ++FGKLFGL + +   VS LQ+
Sbjct:    7 PLYLA---TLLTGLLECIGFAGVLFGWTSLLFVFKAENYFSEPCEQDCLLQSNVTGPSDLKAQDEKFSLIFTLASFMNNFMTFPTGYIFDRFKTTVARLIAIFFYTCATIIIAFTSANTAMLLFLAMPMLAVGGILFLITNLQIGNLFGKHRSTIITLYNGAFDSSSAVFLVIKLLYEQGISLRSSFIFMSVCSVWHIARTFLLMPKGHIPYPLPPNYSYGLCSRFGASKKENKAAEHETKELRSKECLPP--KEENSGPEQQQQQEQQQQQQQQQEQHEQHSFRRCA--LSRRFILHVVWLSIIQLWHY-LFIGTLNSLLTKLSGGDKVEVSAYTNAFAITQFF--GVLCAPWNGLLMDRLKQKYQKAAKRTGSSSEAVALCSMVPSLALTSLLSLGFALCASIPVMQLQYATFILQVVSRSFLYGCNAAFLTLAFPSEHFGKLFGLVMALSAIVSLLQF 457          
BLAST of slc43a-1 vs. UniProt/SwissProt
Match: sp|Q8NBI5|S43A3_HUMAN (Solute carrier family 43 member 3 OS=Homo sapiens OX=9606 GN=SLC43A3 PE=1 SV=2)

HSP 1 Score: 55.0694 bits (131), Expect = 1.218e-6
Identity = 32/114 (28.07%), Postives = 62/114 (54.39%), Query Frame = 1
Query: 1006 SVVISPIVGLMIDYQKNRVS-PSKDQEAFYKSILKCFGIPML-ICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
             V+ +P  GL++D  K +    ++   +   ++  C  +P L + SL  L  ++   +P     Y+ ++   ++R F++  NA+++  AFP ++FGKLFGL + +   VS LQ+
Sbjct:  328 GVLCAPWNGLLMDRLKQKYQKEARKTGSSTLAVALCSTVPSLALTSLLCLGFALCASVPILPLQYLTFILQVISRSFLYGSNAAFLTLAFPSEHFGKLFGLVMALSAVVSLLQF 441          
BLAST of slc43a-1 vs. TrEMBL
Match: A0A0H3YKI8 (Slc43a-1 OS=Schmidtea mediterranea OX=79327 GN=slc43a-1 PE=2 SV=1)

HSP 1 Score: 823.157 bits (2125), Expect = 0.000e+0
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 1
Query:   22 MLSETAFNNNQKEIGLILKPKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYXXXXXXXXXXXXXXXXXXXXXXXXXXHPVILLLN 1443
            MLSETAFNNNQKEIGLILKPKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLISASSLLFGCLLFSSCFLIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNIIISIFSGSFSSSAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYILLLNNICNLNVVNFILIAFSILSFIHPVILLLN
Sbjct:    1 MLSETAFNNNQKEIGLILKPKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLISASSLLFGCLLFSSCFLIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNIIISIFSGSFSSSAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYILLLNNICNLNVVNFILIAFSILSFIHPVILLLN 474          
BLAST of slc43a-1 vs. TrEMBL
Match: A0A0H3YJL7 (Slc43a-2 OS=Schmidtea mediterranea OX=79327 GN=slc43a-2 PE=2 SV=1)

HSP 1 Score: 300.056 bits (767), Expect = 1.117e-91
Identity = 186/456 (40.79%), Postives = 292/456 (64.04%), Query Frame = 1
Query:  103 VLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEI--LVTLNGESDNRIRNCKSDSYLMTSIMKQEN-------YKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYXXXXXXXXXXXXXXXXXXXXXXXXXXHPVILLLN 1443
            VL +AEIEM+FYSGI++GLN L+P LK   +F +  +    +ANA+  +QN+ FGY LITGI +  + S PLG+  D++G K TK+  +    FGCLLF+   +I+  ++FPA ++LG+ AQS+ +CN PL  LVP  + +I++++ GSF +S ++  F+  +Y  G  +  S L +  +G  LM      L+  VD+IK K +  + + N      I +   +     +++K+ +       Y++S +P +K IL + YFL QL F +I FFRFSFFLAQLS+Q++  F  +      +L V +YFF+ S+ I+PI G+++D  K R+ P  D    YK+ L  FGIP++IC + N  M+I I++    A+Y+AY++ TV R F+F+L AS+I++AFP KYFG LFG+ +T  G +SF+QY LL+N++ +LN+VN++LIA S  +F+HP++LL+ 
Sbjct:   26 VLFIAEIEMLFYSGILYGLNVLIPSLKREKYFFDLCSPMLHSANASCVQQNQLFGYNLITGITAFSISSLPLGIFFDKFGYKYTKIFCSLLCFFGCLLFALSSVIKSEMLFPAMLVLGVSAQSLAVCNLPLGGLVPDHETLIVALYVGSFDTSVIVFIFINLSYLSGFSLLASFLIMAFTGLALMELDAFLLMGAVDQIKVKSLKSVTSKNKIGMEDIASGTDEVQASGAVIKEPDTLASLNAYRDSIYPGLKQILSSRYFLIQLMFYIIVFFRFSFFLAQLSIQLDTGFVSNHNMVLYLLSVSSYFFMTSIGIAPISGIIVDALKARIKPMTDPVQLYKTTLSTFGIPLIICCVTNELMAIFIVVAENWAIYIAYMSLTVARAFLFTLTASFILTAFPVKYFGMLFGITVTTGGVISFIQYALLINDLTSLNIVNYVLIAISAFTFVHPILLLIK 481          
BLAST of slc43a-1 vs. TrEMBL
Match: A0A0H3YKJ2 (Slc43a-6 OS=Schmidtea mediterranea OX=79327 GN=slc43a-6 PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 4.499e-65
Identity = 170/453 (37.53%), Postives = 268/453 (59.16%), Query Frame = 1
Query:   91 VRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISG-FILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVS--PSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYXXXXXXXXXXXXXXXXXXXXXXXXXXHPVILLL 1440
            V CFVL +A IEM F+SG+M G NS++P L +  FF    ++     N+T + +N+ + Y++I+ I S +L +F  G+  D++G   TK +S+    FGCL  +   +  + L++P  ++LG  +Q I + N+ L I   K QNI+ISIFS     SA+++  +   ++ G+ +  S LT+GI G  IL+I S K L   V R+   +     NGESD      +     +      + Y NS++  + +I+ N Y+L  + F  I FFR  FFL+QL++Q+E +F       + +L + ++ F+  ++  P  G ++DY KN +      D E  YK+ LKCF IP +ICS  N+ MSI I+IP   A+YM+++A T+TR F++SL + YI++AFP++YFG+  G   TI G +SFLQY L   +  ++N++N+ILI  S L+FIHPV+LLL
Sbjct:    7 VECFVLFIAMIEMFFFSGLMLGFNSIIPSLMKENFF----SKYCPIRNSTCSARNQIYSYVIISAIISLNLSAFICGVFFDKFGQIYTKYLSSLLFFFGCLFIALSSITVNELLYPGVIILGFCSQFIILYNYSLTISFHKCQNILISIFSSLLDISAVVIGLIGLTHRSGVSIFNSFLTLGIFGSVILLINSTKCL--NVKRLSNPQ----ENGESDKL----EKPQDRLDGPANLQVYINSKYHNLTSIVFNRYYLLHIVFTCIIFFRNIFFLSQLNIQLEIHFRGQPKVIEYLLFISSFVFVFGIIAGPFSGAIVDYLKNNLEYQDKNDSELVYKANLKCFVIPFMICSFMNVIMSIFIMIPQIWAVYMSFIAFTITRSFVYSLYSGYILTAFPKEYFGQASGACTTIWGIISFLQYPLSFKDSMSVNILNYILIGMSCLAFIHPVVLLL 445          
BLAST of slc43a-1 vs. TrEMBL
Match: A0A0H3YFC8 (Slc43a-3 OS=Schmidtea mediterranea OX=79327 GN=slc43a-3 PE=2 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 2.930e-62
Identity = 150/442 (33.94%), Postives = 252/442 (57.01%), Query Frame = 1
Query:   79 PKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTK------QNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVD-------RI----KAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKN----RVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
            P +  +   L    +EM+ +SG+M+GLNSL   L +   F    N +    N +S +      QN  F YI+IT + S  + + P+G + D++G + +KL  +   L  C+LF+  F +    +FPA  L GI +Q++  CN  L  + PK QN+I+S+FSGSF +S++MLTF    +  G+ + TS +T+GI GF +M+ +  FL+ K +       R+    +A+ +L+    E    + +   +   +  + K E   N  +P +++I+ + Y+LFQL    I +FRF+FFL QL++Q+  NF        ++L   NYFF+  ++ISPI G+++D  K     ++  S ++   YK  +K F    LIC + NL +SI +IIPN   +++ YL  T+TR F+FSLN++Y++ AFP ++FG++ G+ + + G +SF+QY
Sbjct:   46 PNFNFKYLTLFFGWVEMVCFSGVMYGLNSLFETLAKENLFVQLCNSSSGQINESSNETLFCSTQNTYFSYIMITMMTSQCVLALPIGYLFDKFGSRISKLFVSIVFLVACILFAISFKVTSYFVFPAAFLFGISSQTLLFCNLALGKIFPKHQNLIVSVFSGSFDTSSVMLTFFTLTFLLGVGLMTSFITLGIFGFCIMLINGIFLMAKANVQNNANQRLNNGERAENLLIQ---EETKDLEDGVIEDKELDLMNKLEALTNRMYPDLRSIVFSKYYLFQLGSFTILYFRFTFFLIQLNLQLHYNFPQQRGLVNNLLSRTNYFFLCGILISPIAGIIVDLSKKSFLKKLPNSINEVLLYKRTMKSFVTSQLICCVCNLIVSITVIIPNVALVHVTYLCVTITRAFLFSLNSAYLLQAFPGRFFGQIMGIIVLVAGLMSFIQY 484          
BLAST of slc43a-1 vs. TrEMBL
Match: A0A0H3YJM1 (Slc43a-7 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=slc43a-7 PE=2 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 3.896e-40
Identity = 129/418 (30.86%), Postives = 213/418 (50.96%), Query Frame = 1
Query:  121 IEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNS---QFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMID-YQK--------NRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQ 1338
            +E+  +SG+  G +SL+PI K+ G F         +       Q   FG    T I    L SF LG+V DR+GI    +++A  L+ G L F+    I + L+F A  LL + A  + + N  L ++   F N+++SI S  +  SA+++T +      G       +     G I+++ S   L ++ + + +K+    +N ES N   N + + ++       + Y  S    FP +K  + +  +L Q+ +  I  FRF+FFL+Q+S      F  D+  A  +L V N FFI S++ISPI G+++D YQK        N+V   ++    Y  + +CF +P +ICS A +  S L+ + N+ + Y+ + + TV +  +F++NASYI SAFP+KYFG L G      G ++ LQ
Sbjct:    2 VELSLFSGVFCGFSSLMPICKDFGVFSQCCGTQPGSEGVRCAAQEACFGRAFSTWIVVRALMSFVLGMVFDRFGIGIIGVVAAVCLMGGFLCFAFTP-IFNWLLFIAGSLLTMSAHCLALSNIGLTVIFAGFSNMLVSIISSLYDVSAIVMTMLKVFTDLGAPFWLLCIIHASIGVIIILGSLTILKNRANEVISKDKGEEVNIESINTESNPEGNKHI------NDLYNQSVEETFPTVKACVLSPEYLIQMAYFAIMLFRFTFFLSQMSSHFSYLFPTDQAIANHLLSVSNTFFIGSILISPIFGVLLDKYQKYTIKSLAENKVESPRE---IYNRVNRCFFVPSIICSCAMIVSSSLMFVSNQYSFYVVFSSITVMKSLLFAINASYIFSAFPKKYFGTLIGAVFLTTGFLNLLQ 409          
BLAST of slc43a-1 vs. Ensembl Cavefish
Match: slc43a3a (solute carrier family 43 member 3 [Source:NCBI gene;Acc:103029120])

HSP 1 Score: 85.8853 bits (211), Expect = 2.252e-17
Identity = 103/447 (23.04%), Postives = 194/447 (43.40%), Query Frame = 1
Query:  103 VLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQ-FNRTYDNANATS--TKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLM-----------------------TSIMKQENYKNSQFP-----RMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIP---SVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
             L    +E + ++G++FG  SL+ +LK +G+F +   N T  N    +  + Q+E F  I        +  + P G + DR+G   T+L++      G LL +        L FPA   + +      + N  +  L    ++ II++++G+F SS+ +   +    ++GI + +S L        L +C    LL  +  +    I   +       +   KS+SY +                       + ++K EN   S  P       K+ + + +FL+ L ++ +   R   F+  L+  + +  + D     ++    N F I     +  +P  GL++D  K +     + E   ++ L+   + + I SL  L  SI   +P     Y+ ++   + R F++  NA++I  AFP ++FGKL+GL +++   VS LQY
Sbjct:   17 TLATGLLECLCFAGVVFGWASLVFVLKNDGYFSDLCVNVTEANGTTVTDCSAQDERFSLIFTVASFMNNFLTLPNGFIFDRFGTMATRLLAIFLYTLGTLLVALSSKALSFLFFPAISFIAVGGILFLLTNIQVGNLFGTHRSTIITLYNGAFDSSSAVFLIIKVLREKGISLRSSFL-------FLSVCDVIHLLRTLFLMPKSHIPYPVPENYKYGVTCGKSNSYNVEEFEATRELKYVADTTETAQDRESELLKCENSAPSVEPTGKDNSFKSCVLSWFFLWHLIWLSVMQLRHYLFIGTLNPMLNRLANQDP---DTVSQYTNAFAITQLTGIFCAPWNGLLMDRHKGKALKPGETEK--EADLRSSVLSLFITSLQCLLFSICATVPLLPLQYITFILQVLNRSFLYGGNAAFISIAFPAQHFGKLYGLVMSLSAVVSLLQY 451          
BLAST of slc43a-1 vs. Ensembl Cavefish
Match: slc43a3a (solute carrier family 43 member 3 [Source:NCBI gene;Acc:103029120])

HSP 1 Score: 85.8853 bits (211), Expect = 2.252e-17
Identity = 103/447 (23.04%), Postives = 194/447 (43.40%), Query Frame = 1
Query:  103 VLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQ-FNRTYDNANATS--TKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLM-----------------------TSIMKQENYKNSQFP-----RMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIP---SVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
             L    +E + ++G++FG  SL+ +LK +G+F +   N T  N    +  + Q+E F  I        +  + P G + DR+G   T+L++      G LL +        L FPA   + +      + N  +  L    ++ II++++G+F SS+ +   +    ++GI + +S L        L +C    LL  +  +    I   +       +   KS+SY +                       + ++K EN   S  P       K+ + + +FL+ L ++ +   R   F+  L+  + +  + D     ++    N F I     +  +P  GL++D  K +     + E   ++ L+   + + I SL  L  SI   +P     Y+ ++   + R F++  NA++I  AFP ++FGKL+GL +++   VS LQY
Sbjct:   17 TLATGLLECLCFAGVVFGWASLVFVLKNDGYFSDLCVNVTEANGTTVTDCSAQDERFSLIFTVASFMNNFLTLPNGFIFDRFGTMATRLLAIFLYTLGTLLVALSSKALSFLFFPAISFIAVGGILFLLTNIQVGNLFGTHRSTIITLYNGAFDSSSAVFLIIKVLREKGISLRSSFL-------FLSVCDVIHLLRTLFLMPKSHIPYPVPENYKYGVTCGKSNSYNVEEFEATRELKYVADTTETAQDRESELLKCENSAPSVEPTGKDNSFKSCVLSWFFLWHLIWLSVMQLRHYLFIGTLNPMLNRLANQDP---DTVSQYTNAFAITQLTGIFCAPWNGLLMDRHKGKALKPGETEK--EADLRSSVLSLFITSLQCLLFSICATVPLLPLQYITFILQVLNRSFLYGGNAAFISIAFPAQHFGKLYGLVMSLSAVVSLLQY 451          
BLAST of slc43a-1 vs. Ensembl Cavefish
Match: slc43a3b (solute carrier family 43 member 3 [Source:NCBI gene;Acc:103033106])

HSP 1 Score: 67.0106 bits (162), Expect = 2.744e-11
Identity = 86/390 (22.05%), Postives = 169/390 (43.33%), Query Frame = 1
Query:  244 TKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRI---------------KAKEILVTLNGESDNRIRNCKSD------SYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIP---SVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
            + Q+E F  I        + F+ P G + D  G   T+L+  S    G LL +       IL++PA   + +      + N  +  L    ++ II++++G+F SS+ +   V   +Q GI + + ++ +     + +I  R FLL     I               KA+      N  ++  I++ + +      S   T   K E     +    K+ + + +FL+ L ++ +   R   F+  L+  + +    D     +     N F I     ++ +P  GL++D  K +  P ++ ++  ++ LK   + + + +L  +  S+   IP     Y+ ++   + R F++  NA++I  AFP  +FGK++G  + +   VS LQY
Sbjct:   65 SGQDEQFSIIFTVASFMNNFFTLPNGFLFDHCGTTVTRLVGISIYTTGALLIAFSDAALSILLYPALSFIAVGGILFLVTNMQVGNLFGTHRSTIITLYNGAFDSSSAVFLVVKLLFQDGISLRSCLIFLSSCSVLHLI--RTFLLLPRSHIPYPLPENYSYGLTCGKAERSPTPKNRTANGNIQDSQVNERTPFHSENSTEASKTETAVTER--SFKSCILSWFFLWHLLWLSVMQLRHYLFIGTLNPMLNRLTDGDPALVSTYT---NAFAITQLCGILCAPWNGLIMDRHKRK--PREEGQSETEADLKAAILSLCLTALQCVLFSLCAAIPVLPLQYLTFILQVLNRSFLYGGNAAFISLAFPPAHFGKVYGTVMALSAVVSLLQY 445          
BLAST of slc43a-1 vs. Ensembl Cavefish
Match: slc43a3b (solute carrier family 43 member 3 [Source:NCBI gene;Acc:103033106])

HSP 1 Score: 66.6254 bits (161), Expect = 2.898e-11
Identity = 86/390 (22.05%), Postives = 169/390 (43.33%), Query Frame = 1
Query:  244 TKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRI---------------KAKEILVTLNGESDNRIRNCKSD------SYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIP---SVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
            + Q+E F  I        + F+ P G + D  G   T+L+  S    G LL +       IL++PA   + +      + N  +  L    ++ II++++G+F SS+ +   V   +Q GI + + ++ +     + +I  R FLL     I               KA+      N  ++  I++ + +      S   T   K E     +    K+ + + +FL+ L ++ +   R   F+  L+  + +    D     +     N F I     ++ +P  GL++D  K +  P ++ ++  ++ LK   + + + +L  +  S+   IP     Y+ ++   + R F++  NA++I  AFP  +FGK++G  + +   VS LQY
Sbjct:   65 SGQDEQFSIIFTVASFMNNFFTLPNGFLFDHCGTTVTRLVGISIYTTGALLIAFSDAALSILLYPALSFIAVGGILFLVTNMQVGNLFGTHRSTIITLYNGAFDSSSAVFLVVKLLFQDGISLRSCLIFLSSCSVLHLI--RTFLLLPRSHIPYPLPENYSYGLTCGKAERSPTPKNRTANGNIQDSQVNERTPFHSENSTEASKTETAVTER--SFKSCILSWFFLWHLLWLSVMQLRHYLFIGTLNPMLNRLTDGDPALVSTYT---NAFAITQLCGILCAPWNGLIMDRHKRK--PREEGQSETEADLKAAILSLCLTALQCVLFSLCAAIPVLPLQYLTFILQVLNRSFLYGGNAAFISLAFPPAHFGKVYGTVMALSAVVSLLQY 445          
BLAST of slc43a-1 vs. Ensembl Medaka
Match: slc43a3a (solute carrier family 43 member 3-like [Source:NCBI gene;Acc:101160876])

HSP 1 Score: 100.138 bits (248), Expect = 6.194e-22
Identity = 104/422 (24.64%), Postives = 201/422 (47.63%), Query Frame = 1
Query:  106 LIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIM-KQENYKNSQ-------FPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKN--RVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
            L+   +E + ++G++FG  SL+ +LKE+GFF ++   +    N+  T Q+E F  +        +  S   G + DR+G   T+L++      G LL +    +  +++FPA   +G+    + I N  +  L P  ++ II+I++G+F SS+ +   +   +++G+ + +S L        L ICS   L+  V  +    I   L       +   ++++Y +  I  ++E    SQ           ++ + + +FL+ L ++ I   R   F+  L+  +E+  ++D               +  V+ +P  G ++D  K   R +   +QEA  +S   C  + + + SL +L  S+ + IP     Y+ ++   + R F++  NA++I  AFP  +FGKL+GL +++    S LQY
Sbjct:   16 LVSGMLECLCFAGVVFGFASLVFVLKEDGFF-SELCISNLEINSNCTSQDEQFSLVFTIASFLNNFLSLVNGFLFDRFGTFMTRLLAIFVYTTGTLLVAFSTEL-SLMLFPALSCIGVGGMLLLITNMQVGNLFPAHRSTIITIYNGAFDSSSAVFLLIKVLHEQGVSLRSSFL-------FLSICSIIHLMRTVLLMPKTHIPHPLPDSYTYGVNCGRANAYDVEQIDDRREVMMRSQKLLSICSVRSFQSCVLSWFFLWHLVWLSIMQLRLYLFIGTLNPTLERLANNDPGLVSRYTNAFAMTQLCGVLCAPWNGFLLDRHKGKPRAAGETEQEADLRS--SC--LSLFLTSLQSLVFSVCVSIPVLPLQYLTFILQVLNRSFLYGGNAAFISIAFPACHFGKLYGLMMSLSAVFSLLQY 424          
BLAST of slc43a-1 vs. Ensembl Medaka
Match: slc43a3b (solute carrier family 43 member 3 [Source:NCBI gene;Acc:101166393])

HSP 1 Score: 77.411 bits (189), Expect = 1.223e-14
Identity = 97/436 (22.25%), Postives = 184/436 (42.20%), Query Frame = 1
Query:   94 RCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQ-FNRTYDNANAT--STKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNS------------QFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIP---SVVISPIVGLMIDYQKN--RVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQY 1341
            R    I   +E + ++G +FG  SL+ +LKE G+F +   N T  N   +   + Q+E F  +        +  + P G + DR+G    +L        G L+ S        L+FPA  LL +      + N  +  L    ++ II++++G+F SS+ +   +   ++ GI +  S L        L  CS   +L  +  +    I   L       + NC       T +     +K +            Q    +  + + +F++ L ++ +   R   F+  L+  +++  + + +         N F +     V+ +P  GL++D  K+  R +   +QEA  ++ +    +  L C L     S+    P+    Y+ ++   + R F++  NA+++  AFP  +FGKL+GL + +    S LQY
Sbjct:   12 RGLTFITGLVECLCFAGAVFGWASLVFVLKEEGYFSSMCVNTTTVNGTQSLDCSAQDEQFSLVFTIASFLNNFLTLPNGFLFDRFGTTVARLFGIFLYTMGTLMVSFSTAALGYLLFPALSLLAVGGILFLMTNMQVGNLFGSRRSTIITLYNGAFDSSSALFLIIKLLHEAGISLRGSFL-------FLSACSVAHILRTLFLLPRNFIPYPLPDHYTYGM-NCGKSETERTEVATNSPHKTAEEAPLTKDAPVKQERSFRECVMSRFFVWHLLWLSVMQLRHYLFIGTLNPMLQRLTAGESSLVSQYT---NAFAVTQLCGVLCAPWNGLIMDRHKSKPRAAGETEQEADLRASVLSLFLTALQCVL----FSVCASSPSLPLQYLTFVLQVLNRSFLYGGNAAFLSVAFPSCHFGKLYGLVMALSAVFSILQY 432          
BLAST of slc43a-1 vs. Planmine SMEST
Match: SMESG000043545.1 (SMESG000043545.1)

HSP 1 Score: 813.913 bits (2101), Expect = 0.000e+0
Identity = 470/474 (99.16%), Postives = 473/474 (99.79%), Query Frame = 1
Query:   22 MLSETAFNNNQKEIGLILKPKYLVRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYXXXXXXXXXXXXXXXXXXXXXXXXXXHPVILLLN 1443
            MLSETAFNNNQKEIGLILKPKYLVRCFVLIVAEIEMIFYSGI+FGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLISASSLLFGCLLFSSCFLIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNIIISIFSGSFSSSA+MLTFV YAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYILLLNNICNLNVVNFILIAFSILSFIHP+ILLLN
Sbjct:    1 MLSETAFNNNQKEIGLILKPKYLVRCFVLIVAEIEMIFYSGILFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLISASSLLFGCLLFSSCFLIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNIIISIFSGSFSSSAIMLTFVFYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYILLLNNICNLNVVNFILIAFSILSFIHPLILLLN 474          
BLAST of slc43a-1 vs. Planmine SMEST
Match: SMESG000043543.1 (SMESG000043543.1)

HSP 1 Score: 301.982 bits (772), Expect = 5.807e-96
Identity = 188/456 (41.23%), Postives = 291/456 (63.82%), Query Frame = 1
Query:  103 VLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEI--LVTLNGESDNRIRNCKSDSYLMTSIMKQEN-------YKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYXXXXXXXXXXXXXXXXXXXXXXXXXXHPVILLLN 1443
            VL +AEIEM+FYSGI++GLN L+P LK   +F +  +    +ANA+  KQN+ FGY LITGI +  + S PLG+  D++G K TK+  +    FGCLLF+   +I+  ++FPA ++LG+ AQS+ +CN PL  LVP  + +I++++ GSF +S ++  F+   Y  G  +  S L +  +G  LM      L+  VD+IKAK +    + N      I +   +     +++K+ +       Y++S +P +K IL + YFL QL F +I FFRFSFFLAQLS+Q++  F  +      +L V +YFF+ S+ I+PI G+++D  K R+ P  D    YK+ L  FGIP++IC + N  M+I I++    A+Y+AY++ TV R F+F+L AS+I++AFP KYFG LFG+ +T  G +SF+QY LL+N++ +LN+VN++LIA S  +F+HP++LL+ 
Sbjct:   26 VLFIAEIEMLFYSGILYGLNVLIPSLKREKYFFDLCSPMLHSANASCVKQNQLFGYNLITGITAFSISSLPLGIFFDKFGYKYTKIFCSLLCFFGCLLFALSSVIKSEMLFPAMLVLGVSAQSLAVCNLPLGGLVPDHETLIVALYVGSFDTSVIVFIFINLTYLSGFSLLASFLIMAFTGLALMELDAFLLMGAVDQIKAKPLKSFTSNNKIGMEDIASVTDEVQASGAVIKEPDTLANLNAYRDSIYPGLKQILSSRYFLIQLMFYIIVFFRFSFFLAQLSIQLDTGFVSNHNMVLYLLSVSSYFFMTSIGIAPISGIIVDALKARIKPMTDPVQLYKTTLSTFGIPLIICCVTNELMAIFIVVAENWAIYIAYMSLTVARAFLFTLTASFILTAFPVKYFGMLFGITVTTGGVISFIQYALLINDLTSLNIVNYVLIAISAFTFVHPILLLIK 481          
BLAST of slc43a-1 vs. Planmine SMEST
Match: SMESG000043544.1 (SMESG000043544.1)

HSP 1 Score: 298.516 bits (763), Expect = 1.143e-94
Identity = 187/456 (41.01%), Postives = 290/456 (63.60%), Query Frame = 1
Query:  103 VLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEI--LVTLNGESDNRIRNCKSDSYLMTSIMKQEN-------YKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYXXXXXXXXXXXXXXXXXXXXXXXXXXHPVILLLN 1443
            VL +AEIEM+FYSGI++GLN L+P LK   +F +  +    +ANA+  +QN+ FGY LITGI +  + S PLG+  D++G K TK+  +    FGCLLF+   +I+  ++FPA ++LG+ AQS+ +CN PL  LVP  + +I+++++GSF +S ++  F+   Y  G  +  S L +  +G  LM      L+  VD+IKAK +      N      I +   +     +++K+ +       Y+NS +P +K IL + YFL QL F +I FFR SFFLAQLS+Q++  F  +      +L V +YFF+ S+ I+PI G+++D  K R+ P  D    YK+ L  FGIP++IC + N  M+I I++    A+Y+AY++ TV R F+F+L AS+I++AFP KYFG LFG+ +T  G +SF+QY LL+N++ +LN+VN++LIA S  +F+HP++LL+ 
Sbjct:   26 VLFIAEIEMLFYSGILYGLNVLIPSLKREKYFFDLCSPMLHSANASCVQQNQLFGYNLITGITAFSISSLPLGIFFDKFGYKYTKIFCSLLCFFGCLLFALSSVIKSEMLFPAMLVLGVSAQSLAVCNLPLGGLVPDHETLIVALYAGSFDTSVIVFIFINLTYLSGFSLLASFLIMAFTGLALMELDAFLLMGAVDQIKAKSLKSFTPNNTIGMEDIASGTDEVQASGAVIKEPDILASLNAYRNSIYPGLKQILSSRYFLIQLMFYIIVFFRLSFFLAQLSIQLDTGFISNHNMVLYLLSVSSYFFMTSIGIAPISGIIVDALKARIKPMTDPVQLYKTTLSTFGIPLIICCVTNELMAIFIVVAENWAIYIAYMSLTVARAFLFTLTASFILTAFPVKYFGMLFGITVTTGGVISFIQYALLINDLTSLNIVNYVLIAISAFTFVHPILLLIK 481          
BLAST of slc43a-1 vs. Planmine SMEST
Match: SMESG000043557.1 (SMESG000043557.1)

HSP 1 Score: 229.18 bits (583), Expect = 1.251e-68
Identity = 170/453 (37.53%), Postives = 268/453 (59.16%), Query Frame = 1
Query:   91 VRCFVLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISG-FILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVS--PSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYXXXXXXXXXXXXXXXXXXXXXXXXXXHPVILLL 1440
            V CFVL +A IEM F+SG+M G NS++P L +  FF    ++     N+T + +N+ + Y++I+ I S +L +F  G+  D++G   TK +S+    FGCL  +   +  + L++P  ++LG  +Q I + N+ L I   K QNI+ISIFS     SA+++  +   ++ G+ +  S LT+GI G  IL+I S K L   V R+   +     NGESD      +     +      + Y NS++  + +I+ N Y+L  + F  I FFR  FFL+QL++Q+E +F       + +L + ++ F+  ++  P  G ++DY KN +      D E  YK+ LKCF IP +ICS  N+ MSI I+IP   A+YM+++A T+TR F++SL + YI++AFP++YFG+  G   TI G +SFLQY L   +  ++N++N+ILI  S L+FIHPV+LLL
Sbjct:    7 VECFVLFIAMIEMFFFSGLMLGFNSIIPSLMKENFF----SKYCPIRNSTCSARNQIYSYVIISAIISLNLSAFICGVFFDKFGQIYTKYLSSLLFFFGCLFIALSSITVNELLYPGVIILGFCSQFIILYNYSLTISFHKCQNILISIFSSLLDISAVVIGLIGLTHRSGVSIFNSFLTLGIFGSVILLINSTKCL--NVKRLSNPQ----ENGESDKL----EKPQDRLDGPANLQVYINSKYHNLTSIVFNRYYLLHIVFTCIIFFRNIFFLSQLNIQLEIHFRGQPKVIEYLLFISSFVFVFGIIAGPFSGAIVDYLKNNLEYQDKNDSELVYKANLKCFVIPFMICSFMNVIMSIFIMIPQIWAVYMSFIAFTITRSFVYSLYSGYILTAFPKEYFGQASGACTTIWGIISFLQYPLSFKDSMSVNILNYILIGMSCLAFIHPVVLLL 445          
BLAST of slc43a-1 vs. Planmine SMEST
Match: SMESG000075262.1 (SMESG000075262.1)

HSP 1 Score: 169.859 bits (429), Expect = 2.467e-46
Identity = 135/424 (31.84%), Postives = 218/424 (51.42%), Query Frame = 1
Query:  103 VLIVAEIEMIFYSGIMFGLNSLLPILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLGLVMDRYGIKTTKLIXXXXXXXXXXXXXXXXXIRDILIFPAFVLLGIFAQSICICNFPLAILVPKFQNXXXXXXXXXXXXXAMMLTFVLYAYQRGIDVSTSILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDSYLMTSIMKQENYKNS---QFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQLSVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMID-YQK--------NRVSPSKDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVTRCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQ 1338
            +L +  +EM  +SG+ +G NSL+PI K+ G F         N +     Q   FG    T I    L SF LG+V DR+GI+  K+I+A  L+ G L F+    I + L+F A  LL + A  + + N  L ++   F N+++SI S  +  SA+++T +      G       +     G I+++ S   L ++ + + +K+    +N ES N   N + + ++       + Y  S    FP +K  + +  +L Q+ +  I  FRF+FFL+Q+S      F  D+  A  +L V N FFI S+ ISPI G+++D YQK        N+V   ++    Y  +  CF +P +ICS A +  S L+ + N+ + Y+ + + TV +  +F++NASYI SAFP+KYFG L G      G ++ LQ
Sbjct:    9 ILALMVVEMSLFSGVFYGFNSLIPIYKDFGVFSQYCETQPRNESIRCAAQETYFGRAFSTWIIVQALMSFVLGMVFDRFGIRIIKVIAAICLMGGFLCFAFTP-IFNWLLFIAGSLLTMSAHCLALSNIGLTVIFAGFSNMLVSIISSLYDVSAIVMTMLKVFTDLGAPFWLLCIIHASIGVIIILGSLTILKNRANEVISKDKGEEVNIESINTESNPEGNKHI------NDLYNQSVEETFPTVKACVLSPEYLIQMAYFAIMLFRFTFFLSQMSSHFSYLFPTDQAIANHLLSVSNTFFIGSIFISPIFGVLLDKYQKYTIKSLAENKVESPRE---IYNRVNGCFFVPSIICSCAMIVSSSLMFVSNQYSFYVVFSSITVMKSLLFAINASYIFSAFPKKYFGTLIGAVFLTTGFLNLLQ 422          
The following BLAST results are available for this feature:
BLAST of slc43a-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SLC43A32.536e-728.07solute carrier family 43 member 3 [Source:HGNC Sym... [more]
SLC43A32.536e-728.07solute carrier family 43 member 3 [Source:HGNC Sym... [more]
SLC43A32.536e-728.07solute carrier family 43 member 3 [Source:HGNC Sym... [more]
SLC43A32.536e-728.07solute carrier family 43 member 3 [Source:HGNC Sym... [more]
SLC43A33.023e-728.07solute carrier family 43 member 3 [Source:HGNC Sym... [more]
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BLAST of slc43a-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of slc43a-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of slc43a-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
slc43a3b2.662e-1722.86solute carrier family 43 member 3b [Source:ZFIN;Ac... [more]
slc43a3b2.662e-1722.86solute carrier family 43 member 3b [Source:ZFIN;Ac... [more]
slc43a3a3.582e-1722.40solute carrier family 43 member 3a [Source:ZFIN;Ac... [more]
slc43a3a3.582e-1722.40solute carrier family 43 member 3a [Source:ZFIN;Ac... [more]
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BLAST of slc43a-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
slc43a26.125e-628.70solute carrier family 43 (amino acid system L tran... [more]
slc43a26.637e-628.70solute carrier family 43 (amino acid system L tran... [more]
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BLAST of slc43a-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Slc43a31.431e-1022.34solute carrier family 43, member 3 [Source:MGI Sym... [more]
Slc43a11.642e-831.53solute carrier family 43, member 1 [Source:MGI Sym... [more]
Slc43a11.041e-631.53solute carrier family 43, member 1 [Source:MGI Sym... [more]
Slc43a11.041e-631.53solute carrier family 43, member 1 [Source:MGI Sym... [more]
Slc43a11.084e-631.53solute carrier family 43, member 1 [Source:MGI Sym... [more]
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BLAST of slc43a-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|A2AVZ9|S43A3_MOUSE1.001e-922.34Solute carrier family 43 member 3 OS=Mus musculus ... [more]
sp|Q8NBI5|S43A3_HUMAN1.218e-628.07Solute carrier family 43 member 3 OS=Homo sapiens ... [more]
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BLAST of slc43a-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0H3YKI80.000e+0100.00Slc43a-1 OS=Schmidtea mediterranea OX=79327 GN=slc... [more]
A0A0H3YJL71.117e-9140.79Slc43a-2 OS=Schmidtea mediterranea OX=79327 GN=slc... [more]
A0A0H3YKJ24.499e-6537.53Slc43a-6 OS=Schmidtea mediterranea OX=79327 GN=slc... [more]
A0A0H3YFC82.930e-6233.94Slc43a-3 OS=Schmidtea mediterranea OX=79327 GN=slc... [more]
A0A0H3YJM13.896e-4030.86Slc43a-7 (Fragment) OS=Schmidtea mediterranea OX=7... [more]
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BLAST of slc43a-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
slc43a3a2.252e-1723.04solute carrier family 43 member 3 [Source:NCBI gen... [more]
slc43a3a2.252e-1723.04solute carrier family 43 member 3 [Source:NCBI gen... [more]
slc43a3b2.744e-1122.05solute carrier family 43 member 3 [Source:NCBI gen... [more]
slc43a3b2.898e-1122.05solute carrier family 43 member 3 [Source:NCBI gen... [more]
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BLAST of slc43a-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of slc43a-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of slc43a-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of slc43a-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
slc43a3a6.194e-2224.64solute carrier family 43 member 3-like [Source:NCB... [more]
slc43a3b1.223e-1422.25solute carrier family 43 member 3 [Source:NCBI gen... [more]
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BLAST of slc43a-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000043545.10.000e+099.16SMESG000043545.1[more]
SMESG000043543.15.807e-9641.23SMESG000043543.1[more]
SMESG000043544.11.143e-9441.01SMESG000043544.1[more]
SMESG000043557.11.251e-6837.53SMESG000043557.1[more]
SMESG000075262.12.467e-4631.84SMESG000075262.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30035121
GenBankAKN21683.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035121 ID=SMED30035121|Name=slc43a-1|organism=Schmidtea mediterranea sexual|type=transcript|length=1746bp
AATAACCTTAATAAAATTTAAATGTTATCAGAAACAGCTTTCAATAATAA
CCAGAAAGAAATTGGTTTAATATTAAAACCAAAATATTTAGTTAGATGTT
TTGTATTAATTGTCGCAGAAATCGAGATGATTTTCTACAGTGGAATTATG
TTCGGATTGAATTCTCTACTGCCGATTCTGAAAGAGAATGGATTTTTCCA
CAACCAATTCAATCGAACTTATGACAATGCCAATGCGACTTCTACGAAAC
AGAATGAAACTTTCGGCTACATTCTAATCACAGGAATAGCCTCATGCCAT
TTGTTTTCTTTCCCGCTGGGATTGGTAATGGACAGATATGGCATCAAAAC
GACAAAATTGATATCAGCTTCATCGCTTCTATTCGGATGTTTGCTTTTCT
CCTCGTGCTTTCTAATTAGAGACATATTAATATTCCCGGCATTTGTTTTA
CTTGGGATTTTCGCTCAATCTATTTGTATTTGCAATTTTCCATTAGCTAT
TCTTGTTCCAAAATTTCAAAATATTATCATTTCGATCTTTTCGGGTTCTT
TCAGTTCGTCTGCAATGATGCTGACCTTTGTTTTGTATGCTTATCAAAGA
GGAATTGACGTTTCTACATCAATATTGACCATTGGGATATCAGGGTTTAT
TCTAATGATATGCAGCAGGAAATTCCTGTTGGATAAGGTCGATCGGATTA
AGGCTAAGGAAATATTGGTGACTTTAAATGGTGAATCTGATAACAGAATT
AGAAATTGTAAATCTGATTCATATTTAATGACCTCAATTATGAAGCAGGA
AAATTATAAAAATTCTCAATTTCCCCGAATGAAAACTATCCTAAGGAATC
ATTATTTCTTGTTCCAGCTGTTCTTTATGGTAATTACATTTTTTCGGTTT
TCGTTTTTTCTAGCTCAACTGAGTGTTCAAATGGAAAAGAATTTCTCAGA
TGACGAAACGAAAGCAAAATCTATTTTACTTGTGGGAAACTACTTTTTTA
TTCCTAGCGTCGTTATTTCTCCGATAGTCGGACTGATGATTGACTATCAG
AAAAATCGCGTCTCACCAAGCAAGGACCAAGAGGCATTTTATAAATCAAT
TTTGAAATGCTTCGGAATTCCGATGCTGATCTGCAGCCTGGCAAATTTAT
CCATGAGCATCCTGATTATCATTCCAAATGAGGTGGCAATGTACATGGCT
TATTTGGCTTGCACAGTAACCCGGTGTTTCATGTTTTCTCTCAATGCTAG
TTACATAATTTCCGCTTTTCCTCGGAAATATTTCGGAAAATTATTCGGAC
TTAATATTACGATCTGCGGATGCGTTAGTTTCCTCCAGTACATTTTACTT
TTAAACAATATTTGCAATCTGAACGTTGTCAATTTTATTCTCATAGCGTT
CTCGATTCTTTCATTTATTCACCCAGTGATTCTGCTATTAAACTAGGAGT
CGTTCAGATTTCGATAAAAATTGTGCATTGCAATTTCTGCTTTTCATCGA
AAATTTGTTTTTCAGATAATGTAAATATTTAATAAATTAATGAAATTTAC
ATTTTTACGAATATATTCGCAATTTGATTGGTTAGAATTGATGCTCGCGG
AATTCGGGTAAGATTAGCAAATTCAACCAATAAAAATGAAGCAAAAAAGA
AAAGTGTAATTAAAAAGTTTTTTTAAAAAACGAATTAAAAATATACAAGT
GATTTTTTTAAAGTTATTTTGCTGGTTTCTACTACAATTGCAGGAC
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protein sequence of SMED30035121-orf-1

>SMED30035121-orf-1 ID=SMED30035121-orf-1|Name=SMED30035121-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=475bp
MLSETAFNNNQKEIGLILKPKYLVRCFVLIVAEIEMIFYSGIMFGLNSLL
PILKENGFFHNQFNRTYDNANATSTKQNETFGYILITGIASCHLFSFPLG
LVMDRYGIKTTKLISASSLLFGCLLFSSCFLIRDILIFPAFVLLGIFAQS
ICICNFPLAILVPKFQNIIISIFSGSFSSSAMMLTFVLYAYQRGIDVSTS
ILTIGISGFILMICSRKFLLDKVDRIKAKEILVTLNGESDNRIRNCKSDS
YLMTSIMKQENYKNSQFPRMKTILRNHYFLFQLFFMVITFFRFSFFLAQL
SVQMEKNFSDDETKAKSILLVGNYFFIPSVVISPIVGLMIDYQKNRVSPS
KDQEAFYKSILKCFGIPMLICSLANLSMSILIIIPNEVAMYMAYLACTVT
RCFMFSLNASYIISAFPRKYFGKLFGLNITICGCVSFLQYILLLNNICNL
NVVNFILIAFSILSFIHPVILLLN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR027197SLC43A3
IPR036259MFS_trans_sf
IPR020846MFS_dom
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 268..474
e-value: 8.5E-9
score: 36.9
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 23..231
e-value: 8.1E-6
score: 27.3
NoneNo IPR availableCDDcd17370MFS_MJ1317_likecoord: 94..147
e-value: 0.00401988
score: 37.1375
NoneNo IPR availableTMHMMTMhelixcoord: 451..473
NoneNo IPR availableTMHMMTMhelixcoord: 201..220
NoneNo IPR availableTMHMMTMhelixcoord: 363..385
NoneNo IPR availableTMHMMTMhelixcoord: 114..136
NoneNo IPR availableTMHMMTMhelixcoord: 80..102
NoneNo IPR availableTMHMMTMhelixcoord: 390..412
NoneNo IPR availableTMHMMTMhelixcoord: 278..300
NoneNo IPR availableTMHMMTMhelixcoord: 140..162
NoneNo IPR availableTMHMMTMhelixcoord: 424..441
NoneNo IPR availableTMHMMTMhelixcoord: 22..44
NoneNo IPR availableTMHMMTMhelixcoord: 169..191
NoneNo IPR availableTMHMMTMhelixcoord: 320..342
IPR027197Solute carrier family 43 member 3PANTHERPTHR20765FAMILY NOT NAMEDcoord: 25..472
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 77..470