DNA-directed RNA polymerase III subunit RPC3

Overview
NameDNA-directed RNA polymerase III subunit RPC3
Smed IDSMED30035115
Length (bp)1592
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA-directed RNA polymerase III subunit RPC3 (SMED30035115) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA-directed RNA polymerase III subunit RPC3 (SMED30035115) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA-directed RNA polymerase III subunit RPC3 (SMED30035115) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA-directed RNA polymerase III subunit RPC3 (SMED30035115) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035115

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30035115SMESG000050553.1 SmedASXL_014711SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Human
Match: POLR3C (RNA polymerase III subunit C [Source:HGNC Symbol;Acc:HGNC:30076])

HSP 1 Score: 60.8474 bits (146), Expect = 3.480e-9
Identity = 56/260 (21.54%), Postives = 118/260 (45.38%), Query Frame = 2 
Query:  761 EPVTSYEDPMSLTLFSKKVKKRLA---DFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEV-EKKRVVLAHVIENSEKIKLV------KTDEEDPIKD----ITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQ 1498
            E  TS   P +  L S ++ + L    +     L++ L LL+ D+ + ++ +  +S G +   +  + +  L    +  +VQ  +G    RIF  VL   +IE+    +  ++ A +   +L  M++  F++  E  KT  +       L+ V++L   R     CY+ +  L  R++ E  E KR     ++E S++++ +         EE  +++    IT  + + +E + +  +K D+ E  +   + L  ++I+
Sbjct:  275 EITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLA-DDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYKMLSENFMSLQEIPKTPDHAPSRTFYLYTVNILSAARMLLHRCYKSIANLIERRQFETKENKR-----LLEKSQRVEAIIASMQATGAEEAQLQEIEEMITAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIE 528
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Celegans
Match: let-611 (pep chromosome:WBcel235:I:6255035:6257635:1 gene:WBGene00016750.1 transcript:C48E7.2.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:let-611)

HSP 1 Score: 51.9878 bits (123), Expect = 9.142e-7
Identity = 93/423 (21.99%), Postives = 163/423 (38.53%), Query Frame = 2 
Query:  161 ILSCLENLIKYNIVIVNYDELHTDSYQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEEILENVPS-----NFQKRKTTKRNPNVTYTD--EIKRILSTKENPTDSRKRVFEKIDETESNIDKE--LRINNERFRVLSRNEIFIQDAILTLGEDAGM-----------------------------VFKIFIDSFDEEPVTSYEDPMSLTLFSKKVKKRLADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEVEKKRVVL 1315
            I   +  LI +  V  + D  +  +Y    +     I S R  LI    +    + I       G L  SE + +I+   E   + + +  + E L     L  VPS     +   +  T  +P    T+  E K   ST ++ +++RKR   K D T  + D     RIN  RF    R+E+ I D ++  G    M                             +FKI  +    +P++      SLT   + VK+   +     +E    +L  DES   I +   SNG +   D  R + Q+       +++  +    +R+   + N  Y++E     L +MS  +   +L A+V  G++      ++  +       L++VDL + +R   E   + +  L VR+  E  + + VL
Sbjct:   53 IRKSVSTLINFGFVTFHLDTNNRTTYVADSDQIQHVISSPRACLIAKTLYGPAAESICVELISQGRLTVSETIRRIQATDETAVFSD-IKKSFEELAAAHFLIRVPSVESEIHGCPQFVTNFDPFAISTNIMEKKEGTSTSDSTSNNRKR---KADSTPEDGDAGQYWRINWIRFNGYLRDELII-DYLIGGGSKGTMTNLNVKKEETIEDPQGDRTQYITQNVAHLMFKI--NETRAKPMSMESITASLTDLQRLVKENGLNLAKPDIELACQILV-DESDGIIRKLGESNGGIFIVDIRRAIRQICRHHCESLIREQFEGRAIRVMRLLENRHYLDEEQVEKLSMMSGKEAREMLYALVEEGYVFNKPVGRSNDFQPARTFYLYYVDLPRTIRGLVEYTCKLVRNLIVRQRHERTENKSVL 467
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Celegans
Match: let-611 (pep chromosome:WBcel235:I:6255035:6257791:1 gene:WBGene00016750.1 transcript:C48E7.2.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:let-611)

HSP 1 Score: 51.9878 bits (123), Expect = 9.142e-7
Identity = 93/423 (21.99%), Postives = 163/423 (38.53%), Query Frame = 2 
Query:  161 ILSCLENLIKYNIVIVNYDELHTDSYQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEEILENVPS-----NFQKRKTTKRNPNVTYTD--EIKRILSTKENPTDSRKRVFEKIDETESNIDKE--LRINNERFRVLSRNEIFIQDAILTLGEDAGM-----------------------------VFKIFIDSFDEEPVTSYEDPMSLTLFSKKVKKRLADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEVEKKRVVL 1315
            I   +  LI +  V  + D  +  +Y    +     I S R  LI    +    + I       G L  SE + +I+   E   + + +  + E L     L  VPS     +   +  T  +P    T+  E K   ST ++ +++RKR   K D T  + D     RIN  RF    R+E+ I D ++  G    M                             +FKI  +    +P++      SLT   + VK+   +     +E    +L  DES   I +   SNG +   D  R + Q+       +++  +    +R+   + N  Y++E     L +MS  +   +L A+V  G++      ++  +       L++VDL + +R   E   + +  L VR+  E  + + VL
Sbjct:   53 IRKSVSTLINFGFVTFHLDTNNRTTYVADSDQIQHVISSPRACLIAKTLYGPAAESICVELISQGRLTVSETIRRIQATDETAVFSD-IKKSFEELAAAHFLIRVPSVESEIHGCPQFVTNFDPFAISTNIMEKKEGTSTSDSTSNNRKR---KADSTPEDGDAGQYWRINWIRFNGYLRDELII-DYLIGGGSKGTMTNLNVKKEETIEDPQGDRTQYITQNVAHLMFKI--NETRAKPMSMESITASLTDLQRLVKENGLNLAKPDIELACQILV-DESDGIIRKLGESNGGIFIVDIRRAIRQICRHHCESLIREQFEGRAIRVMRLLENRHYLDEEQVEKLSMMSGKEAREMLYALVEEGYVFNKPVGRSNDFQPARTFYLYYVDLPRTIRGLVEYTCKLVRNLIVRQRHERTENKSVL 467
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Zebrafish
Match: polr3c (polymerase (RNA) III (DNA directed) polypeptide C [Source:NCBI gene;Acc:393201])

HSP 1 Score: 64.3142 bits (155), Expect = 1.987e-10
Identity = 99/529 (18.71%), Postives = 209/529 (39.51%), Query Frame = 2 
Query:   47 IQKNFNENSKNVCQILLSNNWSTLKEIHSELKHLKSKEILSCLENLIKYNIVIVNYDELHTDS---YQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSE----LLDKIRIYIEDDDWI--NLVINATEVLINEEILENVP----------------------SNFQKRKTTKRNPNVTYTDEIKRILSTKENPTDSRKRVFEKIDETESNIDK-ELRINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFIDSFDEEPVTSYEDPMSLTLFSKKVKKRLA---DFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEV-EKKR---------VVLAHV-IENSEKIKLVKTDEEDPIKDITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFI 1495
            +Q++F +  +NV   L+ N   TL+ +  E K L    +   L  L+++ +               Y  + E+ +  +   R++    + + +  ++I E     G +  S     + D++   + +   +  N V++A   L+    L+  P                      SN +      + P ++ +   KR  S+++   D R     K D +E   +    ++N ERF +  R++  I      L + +G + +  +     E  TS     +  L + ++ + L    +     L++ L LL  D+ + ++ +  +S G +   +  R +  L    +  +VQ  +G    RIF  +L   ++E+    +  ++ A +   +L  +++   +   E  KT  +       L+ V+ L   R   + CY+ +  L  R+  E  E KR          +LA +    +E  +L + +E      IT  + + +E +    +K DS E  +   + L  ++I
Sbjct:   13 LQEHFGDVVENVGTHLIRNGVLTLRALAHETK-LALDLVKKSLCVLVQHGMCTFGPGRRGPAGPVEYHINCEHILHMLRYPRYIYTAKSLYGDTGELIVEEILQRGQMTMSSTVKTVADRLTHNMPEGQSMDYNEVVSAFSRLVETHFLQRCPPMASAESSGTGSAETPPAVTQPSNPESHPDCYKLPYISLSGHGKRRRSSEDGEADQRAAKKAKTDSSECGDEGIHWQVNFERFHLHFRDQAIISAVSCKLDQTSGEIVRTMLRM--SEVTTSANAAFTQALSANEIFRALPSNYNIARPILDQYLTLLV-DDPMEFVGKAGDSGGGMFVVNLHRALANLARATLESVVQERFGSRSARIFRLLLRKRHLEQKQVEDFAMIPAKEAKEMLYTLLSENLVQLQEIPKTPDHAPSRTFYLYTVNQLPTARLLLQHCYKTVGNLIERRLYETKENKRLLEKSQRIEAILASLQATGAEATQLTEVEEM-----ITAPERQQLESLRHHINKLDSSENQVDETIFLLESYI 532
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. UniProt/SwissProt
Match: sp|Q2TBL4|RPC3_BOVIN (DNA-directed RNA polymerase III subunit RPC3 OS=Bos taurus OX=9913 GN=POLR3C PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.204e-9
Identity = 71/346 (20.52%), Postives = 151/346 (43.64%), Query Frame = 2 
Query:  515 PNVTYTDEIKRILSTKENPTD--SRKRVFEKIDETESNIDKEL--RINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFIDSFDEEPVTSYEDPMSLTLFSKKVKKRLA---DFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEV-EKKRVVLAHVIENSEKIKLV------KTDEEDPIKD----ITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQ 1498
            P ++   + KR  S+ E+ T     KR  +  D  E   D  +  + N +RF    R++  +      + + +  + +  +     E  T    P +  L S ++ + L    +     L++ L LL+ D+ + ++ +  +S G +   +  + +  L    +  +VQ  +G    RIF  VL   ++E+    +  ++ A +   +L  M++  FI+  E  KT  +       L+ V++L   R     CY+ +  L  R++ E  E KR     ++E S++++ +         EE  +++    IT  + + +E + +  +K D+CE  +   + L  ++I+
Sbjct:  191 PKLSLIGKGKRRRSSDEDATGEPKAKRPKQTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRM--SEVTTPSGAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLA-DDPLEFVGKSGDSGGGMYVINLHKALGSLATATLESVVQERFGSRCARIFRLVLQKKHLEQKQVEDFAMIPAKEAKDMLYKMLSENFISLQEIPKTPDHAPSRTFYLYTVNILSAARMLLHRCYKSVANLIERRQFETKENKR-----LLEKSQRVEAIIASMQATGAEEAQLQEIEEMITAPERQQLETLKRNVNKLDACEIQVDETIFLLESYIE 528
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. UniProt/SwissProt
Match: sp|Q7ZUX1|RPC3_DANRE (DNA-directed RNA polymerase III subunit RPC3 OS=Danio rerio OX=7955 GN=polr3c PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.350e-9
Identity = 99/529 (18.71%), Postives = 209/529 (39.51%), Query Frame = 2 
Query:   47 IQKNFNENSKNVCQILLSNNWSTLKEIHSELKHLKSKEILSCLENLIKYNIVIVNYDELHTDS---YQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSE----LLDKIRIYIEDDDWI--NLVINATEVLINEEILENVP----------------------SNFQKRKTTKRNPNVTYTDEIKRILSTKENPTDSRKRVFEKIDETESNIDK-ELRINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFIDSFDEEPVTSYEDPMSLTLFSKKVKKRLA---DFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEV-EKKR---------VVLAHV-IENSEKIKLVKTDEEDPIKDITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFI 1495
            +Q++F +  +NV   L+ N   TL+ +  E K L    +   L  L+++ +               Y  + E+ +  +   R++    + + +  ++I E     G +  S     + D++   + +   +  N V++A   L+    L+  P                      SN +      + P ++ +   KR  S+++   D R     K D +E   +    ++N ERF +  R++  I      L + +G + +  +     E  TS     +  L + ++ + L    +     L++ L LL  D+ + ++ +  +S G +   +  R +  L    +  +VQ  +G    RIF  +L   ++E+    +  ++ A +   +L  +++   +   E  KT  +       L+ V+ L   R   + CY+ +  L  R+  E  E KR          +LA +    +E  +L + +E      IT  + + +E +    +K DS E  +   + L  ++I
Sbjct:   13 LQEHFGDVVENVGTHLIRNGVLTLRALAHETK-LALDLVKKSLCVLVQHGMCTFGPGRRGPAGPVEYHINCEHILHMLRYPRYIYTAKSLYGDTGELIVEEILQRGQMTMSSTVKTVADRLTHNMPEGQSMDYNEVVSAFSRLVETHFLQRCPPMASAESSGTGSAETPPAVTQPSNPESHPDCYKLPYISLSGHGKRRRSSEDGEADQRAAKKAKTDSSECGDEGIHWQVNFERFHLHFRDQAIISAVSCKLDQTSGEIVRTMLRM--SEVTTSANAAFTQALSANEIFRALPSNYNIARPILDQYLTLLV-DDPMEFVGKAGDSGGGMFVVNLHRALANLARATLESVVQERFGSRSARIFRLLLRKRHLEQKQVEDFAMIPAKEAKEMLYTLLSENLVQLQEIPKTPDHAPSRTFYLYAVNQLPTARLLLQHCYKTVGNLIERRLYETKENKRLLEKSQRIEAILASLQATGAEATQLTEVEEM-----ITAPERQQLESLRHHINKLDSGENQVDETIFLLESYI 532
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. UniProt/SwissProt
Match: sp|Q9BUI4|RPC3_HUMAN (DNA-directed RNA polymerase III subunit RPC3 OS=Homo sapiens OX=9606 GN=POLR3C PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.671e-8
Identity = 56/260 (21.54%), Postives = 118/260 (45.38%), Query Frame = 2 
Query:  761 EPVTSYEDPMSLTLFSKKVKKRLA---DFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEV-EKKRVVLAHVIENSEKIKLV------KTDEEDPIKD----ITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQ 1498
            E  TS   P +  L S ++ + L    +     L++ L LL+ D+ + ++ +  +S G +   +  + +  L    +  +VQ  +G    RIF  VL   +IE+    +  ++ A +   +L  M++  F++  E  KT  +       L+ V++L   R     CY+ +  L  R++ E  E KR     ++E S++++ +         EE  +++    IT  + + +E + +  +K D+ E  +   + L  ++I+
Sbjct:  275 EITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLA-DDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYKMLSENFMSLQEIPKTPDHAPSRTFYLYTVNILSAARMLLHRCYKSIANLIERRQFETKENKR-----LLEKSQRVEAIIASMQATGAEEAQLQEIEEMITAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIE 528
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. UniProt/SwissProt
Match: sp|Q55FJ5|RPC3_DICDI (DNA-directed RNA polymerase III subunit rpc3 OS=Dictyostelium discoideum OX=44689 GN=polr3c PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 8.903e-6
Identity = 38/147 (25.85%), Postives = 66/147 (44.90%), Query Frame = 2 
Query:  953 IVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEVEKKRVVLAHVIENSEKIKLVKTDEEDPIKDITK 1393
            I+  +  K++  I++  +GDNGLR+F  +L    +E      L ++  N+   LL  M+ +  I   E  ++  +F      LF VDL  I+    E  ++ +Y    R + E+E  +  L          KL + DE+   +D  K
Sbjct:  500 IIGIIKQKMVESIIKQKFGDNGLRVFKLLLIKNLLEPKQIAELAMIPPNECKALLFNMMQKNIIRLQEIPRSSDHFANRTFYLFFVDLPTIIATFTEDIFKAIYNTRERLKSELEPHKDALE---------KLTQLDEDQITEDQAK 637
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. TrEMBL
Match: A0A1E1XC86 (RNA polymerase III subunit C3 (Fragment) OS=Amblyomma aureolatum OX=187763 PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.319e-15
Identity = 112/511 (21.92%), Postives = 215/511 (42.07%), Query Frame = 2 
Query:   47 IQKNFNENSKNVCQILLSNNWSTLKEIHSELKHLKSKEILSCLENLIKYNIVIVNYDELHTDSYQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEEILENVPSNFQKRKTTK------RNPNVTYTDEIKRILSTKENPTDSRKRVFEKIDETESNIDKE--------LRINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFI--DSFDEEPVTSYEDPMSLTLFSKKVKKRLADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKE-MEVEKKRVVLAHVIENSEKIKLV---------KTDEEDPIKD-ITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQ 1498
            ++++F    ++V + LL  +  +L EI +   +L   +I   L  LI  N+V  +        Y  + +  +  +   R++      + +  + I E   + G LC S +L ++   ++       V      L+    L  VP      + TK      R  ++    +IK  L  K+N +D+      K  +TE N+D E         R+N +RF    R++  +Q     +G  AG V +  +        P  ++  P+S +   + +KK + +    +L+  L L++ D     +  R +  G +   +    +  L   ++T IVQ  +G    RIF  +L   YIE+     L ++ A         M    FI   E  +T  Y    ++ LFHVD+ ++ R   E CYQ L  L +R++ +  E KR+V     +  +++ L+           ++ + +K+ IT  + + + ++   +SK +  E  +   +++   FIQ
Sbjct:    9 LKEHFGAVVQSVGECLLKVSSRSLVEI-ARATNLPLSKIKKSLVVLIHQNLVEFHAHPRGFGKYSINADRVLCILRYPRYIYCAKLLYGDAGEFIIEEVLLNGRLCMSAVLQRVLERLQGSSTAEEVKQKLADLVKTNFLCRVPYPDPNVEDTKIPTLILREEDMHEIPQIKLYL-LKQNFSDTTGEPPAKKVKTE-NLDGEKQPDEKIYWRVNVDRFHQHLRDQSVVQAVSKRMGNKAGEVVRTMLRLSETRSHPKDTFSAPVSRSEIHQALKKEI-NISQQELDSYLSLIADD--ALLLVRRTDERGGMFSVNLEHAMRLLAEAVLTSIVQDRFGAKSCRIFRLLLEKKYIEQKQIEELAMIPARDAKEWTYKMFQEKFIMAQELPRTPDYAPSRMIYLFHVDIPQLSRQVLEWCYQALTNLVLRRDHITQEYKRLV-----DKKQRVDLIVLSMQQNSAAPEQIEEVKEMITPPEKKQLAYVKHVTSKIELSEVQLDETILVLQLFIQ 508
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. TrEMBL
Match: A0A5N4ARG8 (Uncharacterized protein OS=Photinus pyralis OX=7054 GN=PPYR_07825 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.241e-14
Identity = 109/471 (23.14%), Postives = 209/471 (44.37%), Query Frame = 2 
Query:  149 KSKEILSCLENLIKYNIVIVNYDELHTDS----YQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEEILENVPSNFQKRKTTKRNPNVTYTDEIKRILSTKENPTDSRKRVFEKID---ETESNIDKELRINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFIDSF--DEEPVTSYEDPMSLTLFSKKVKKR-----LADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEA-LVSLFHVDLLKIVRNERETCYQCLYRLGVRK--EMEVEKKRVVLAHVIENSEKIKLVKTDEEDPIKD----ITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQ 1498
            K KE LS L   IKYN+V     +L+ DS    Y  +++N +  +   +F+ ++  KFD  ++++ E   + G +  SEL+ K+   +++   +N+++        +E+ +N  S    +  T+ +  VT  +E+  +      P    K++   ++   ET S+      IN++RF    R+++ +        ++ G + ++ +       +P  S  +P+ +      VKKR     LA F     E+ + +L +D +   I +   ++G     +   I  QL  + + EIV   +     RIF  V    YIE    +NLV++ A ++  +L  ++   F+   E  ++      +   +LFH+ L ++VR   E CY+ LY +  R+  E  + K+ +   H ++       V+    D + D    IT  + E +E I K   K    E  I   L L   +++
Sbjct:   48 KVKEALSVL---IKYNLVTF---KLNVDSSAAIYMLNLKNVLLMLRFPKFINLIKTKFDETYEVLVEEILLKGCVTASELIIKVAKRLQEQKTVNILL--------QELKDNFNSLVTAKYVTRLSTQVTKAEELYLL------PNLDLKQILTVLNGENETLSDAGIYWTINSDRFHQDLRDQLIVNSVSNKFDDNMGELIRLLLQQMYVRTDPWVSSSNPIPILEVKDIVKKRNTHPQLATF----FEQYVNILEQD-ATGIIRKVGEASGGSFQVEIKHIFTQLTWEAVEEIVNEKFDSKASRIFRLVKLRHYIEPEQIQNLVMIPAKEVKRILYMLLEENFLVVNELKRSATSLGPSKSFTLFHIRLSQVVRTMLELCYKTLYNIMTRRCHEKYLNKRLIDKKHRVDTITHGMRVQGISGDSVLDIEEMITPPENEILERIGKMMKKLSLTELEIDETLFLLEMYLK 493
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. TrEMBL
Match: A0A1Y1M8V1 (RNA polymerase III subunit C3 (Fragment) OS=Photinus pyralis OX=7054 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.260e-14
Identity = 109/471 (23.14%), Postives = 209/471 (44.37%), Query Frame = 2 
Query:  149 KSKEILSCLENLIKYNIVIVNYDELHTDS----YQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEEILENVPSNFQKRKTTKRNPNVTYTDEIKRILSTKENPTDSRKRVFEKID---ETESNIDKELRINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFIDSF--DEEPVTSYEDPMSLTLFSKKVKKR-----LADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEA-LVSLFHVDLLKIVRNERETCYQCLYRLGVRK--EMEVEKKRVVLAHVIENSEKIKLVKTDEEDPIKD----ITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQ 1498
            K KE LS L   IKYN+V     +L+ DS    Y  +++N +  +   +F+ ++  KFD  ++++ E   + G +  SEL+ K+   +++   +N+++        +E+ +N  S    +  T+ +  VT  +E+  +      P    K++   ++   ET S+      IN++RF    R+++ +        ++ G + ++ +       +P  S  +P+ +      VKKR     LA F     E+ + +L +D +   I +   ++G     +   I  QL  + + EIV   +     RIF  V    YIE    +NLV++ A ++  +L  ++   F+   E  ++      +   +LFH+ L ++VR   E CY+ LY +  R+  E  + K+ +   H ++       V+    D + D    IT  + E +E I K   K    E  I   L L   +++
Sbjct:   66 KVKEALSVL---IKYNLVTF---KLNVDSSAAIYMLNLKNVLLMLRFPKFINLIKTKFDETYEVLVEEILLKGCVTASELIIKVAKRLQEQKTVNILL--------QELKDNFNSLVTAKYVTRLSTQVTKAEELYLL------PNLDLKQILTVLNGENETLSDAGIYWTINSDRFHQDLRDQLIVNSVSNKFDDNMGELIRLLLQQMYVRTDPWVSSSNPIPILEVKDIVKKRNTHPQLATF----FEQYVNILEQD-ATGIIRKVGEASGGSFQVEIKHIFTQLTWEAVEEIVNEKFDSKASRIFRLVKLRHYIEPEQIQNLVMIPAKEVKRILYMLLEENFLVVNELKRSATSLGPSKSFTLFHIRLSQVVRTMLELCYKTLYNIMTRRCHEKYLNKRLIDKKHRVDTITHGMRVQGISGDSVLDIEEMITPPENEILERIGKMMKKLSLTELEIDETLFLLEMYLK 511
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. TrEMBL
Match: G3ML89 (RNA polymerase III subunit C3 OS=Amblyomma maculatum OX=34609 PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 3.217e-14
Identity = 112/520 (21.54%), Postives = 218/520 (41.92%), Query Frame = 2 
Query:   47 IQKNFNENSKNVCQILLSNNWSTLKEIHSELKHLKSKEILSCLENLIKYNIVIVNYDELHTDSYQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEEILENVPSNFQKRKTTKRNPNVTYT---------DEIKRILSTK-------------ENPTDSRKRVFEKIDETESNIDKE-LRINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFI--DSFDEEPVTSYEDPMSLTLFSKKVKKRLADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKE-MEVEKKRVVLAHVIENSEKIKLV----KTDEEDP-----IKD-ITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQ 1498
            ++++F    ++V + LL     TL EI +   +L   +I   L  LI  N V  +        Y    +  +  +   R++      + +  + I E   + G LC S ++ K+   ++         N+T   + ++ ++ V +NF  R     +PNV  T         +++ +I   K             E P  ++K   E +D  +   +KE  R+N +RF    R+++ +Q     +G  AG V +  +        P   +  P+S +   + +KK + +    +L+  L L++ D     +  R +  G +   +  R V  L   ++T IVQ  +G    RIF  +L   Y E+     L ++ A         M    FI   E  +T  Y    ++ LF+VD+ ++ R   E CYQ L  L  R++ +  E KR+V     +  +++ L+    + +  +P     +K+ IT  + + + ++   +SK +  E  +   +++   +IQ
Sbjct:   13 LKEHFGAVVQSVGECLLKVTSRTLAEI-ARATNLPLSKIKKSLLVLIHQNFVEFHAHPRGFAKYSIHADRVLCMLRYPRYIYCAKLLYGDAGEFIIEEVLLNGRLCMSAVIQKVLDRLQG--------NSTAEEVKQKFVDLVQTNFLVR-VPYPDPNVEGTKIPTLVLREEDVHKIPQVKLYLMKQNYGEASLEPP--AKKIKTENLDGEKQPDEKEYWRVNFDRFHQHLRDQLVVQAVSKRMGSKAGEVVRTMLRLSETRSHPKDVFSVPVSRSEIHQALKKEI-NISSQELDSYLSLIADD--ALLLVRRTDERGGMFSVNLERAVRLLAEAVLTSIVQDRFGAKSCRIFRLLLVKKYTEQKQIEELAMIPARDAKEWTYKMFEEKFIMAQELPRTPDYAPSRMIYLFYVDIPQLSRRVLEWCYQALTNLVQRRDHITQEHKRLV-----DKKQRVDLIVLSMQQNNAEPNQIEEVKEMITPPERKQLAYVKHVTSKIELGEVQLDETILILQLYIQ 512
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. TrEMBL
Match: A0A4D5RHN2 (RNA polymerase III subunit C3 (Fragment) OS=Ixodes scapularis OX=6945 PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 5.362e-14
Identity = 100/476 (21.01%), Postives = 193/476 (40.55%), Query Frame = 2 
Query:  182 LIKYNIVIVNYDELHTDSYQFSIENFISYIISI----------RFLLIVNNKFDNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEEILENVPSNFQKRKTTKRNPNVTYTDEIK--------RILST------KENPTDSRKRVFEKIDETESNIDKEL--RINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFIDSFDE--EPVTSYEDPMSLTLFSKKVKKRLADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEVEKKRVVLAHVIENSEKIKLVKTDEE----DP-----IKD-ITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFI 1495
            LI +NIV           ++   + ++ YII            R+       + +  ++I E     G +C S +L K+   ++       V +    L+    L  VP+  +      + P +   +E          ++LS       +E P   + RV       ES  D+ +  ++N ERF +  R+++ +Q A   LG  AG V +  +   +    P ++   P+S     +++ K ++     +L+  L LL +D     + +R +  G +   D  R +  L        VQ  +G    RIF  +L+  Y+E+     L ++ A     +   M    F+   E  +T  Y    ++ LFHVDL ++ R   E C Q +  L +R+E   ++ R +L    +  +++ L+    E     P     +K+ IT  + + + ++   SSK +  E  +   ++L   ++
Sbjct:    3 LIHHNIV----------DFRLHPKGYVEYIIDADRVLYMLRYPRYTHCAKTLYGDTGELIVEELLQNGRMCMSAVLRKVLGRLQGSHTAEAVRSKFVDLVQTHFLCRVPAP-EPGGPDAKVPTLILLEEDMYSVPKVHLQLLSVAAEDEDQEGPPSKKSRV-------ESQPDEGIYWQVNFERFHLFFRDQLLVQAASRRLGLKAGEVVRTLLRLAETRTHPKSAQSCPVSKQEVHQQLHKEIS-LSQKELDSFLSLLVEDP--LRLVQRSDERGGMFCVDLHRGLRVLAEAFFASTVQDRFGAKSCRIFRLLLSKRYLEQKQIEELAMIPARDAKEMTYKMFQEKFLAAQELPRTPDYAPSRMIYLFHVDLPQLSRQMLEWCCQAVCNLVLRREHICQEHRRLL----DKKQRVDLILASMEHNKASPEQVHEVKEMITPPERKQLAYVKHVSSKMELSEVQLDETMLLLQLYV 453
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Cavefish
Match: polr3c (RNA polymerase III subunit C [Source:NCBI gene;Acc:103022840])

HSP 1 Score: 51.2174 bits (121), Expect = 2.134e-6
Identity = 43/222 (19.37%), Postives = 93/222 (41.89%), Query Frame = 2 
Query:  881 DESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEVEKKRVVLAHVIENSEKIKLVKTDEEDPIKD----------ITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQQKVTYH 1516
            D+ + ++ +  +S G +   +  R +  L    +  +VQ  +G    RIF  +L   ++E+    +  ++ A +   +L  ++++  +   E  KT  +       L+ V+ L   R     CY+ +  L  R+  E ++ R +L    E S++I+ +    +    D          IT  + + +E +    +K DS E  +   + L  ++I    + H
Sbjct:  324 DDPMEFVSKTGDSGGGMYVVNLHRALANLARVTLESVVQERFGSRSARIFRLLLRKRHLEQKQVEDFAMIPAKEAKDMLYTLLSQNLVQLQEIPKTPDFAPSRTFYLYTVNQLPTARLLLHNCYKAVGNLIERRLFETKENRRLL----EKSQRIEAILASLQGGGADAAQLSDVEEMITAPERQQLEALRHHINKLDSSENQVDETIFLLESYINSTQSSH 541
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Sea Lamprey
Match: polr3c (polymerase (RNA) III (DNA directed) polypeptide C [Source:NCBI gene;Acc:393201])

HSP 1 Score: 59.6918 bits (143), Expect = 8.951e-10
Identity = 59/300 (19.67%), Postives = 125/300 (41.67%), Query Frame = 2 
Query:  638 RINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFI--DSFDEEPVTSYEDPMSLTLFSKKVKKRLADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEV-EKKRVVLAHVIENSEKIKLVKTDEEDPIKD----------ITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQ 1498
            R+N ERF     N+  +      + + AG + +  +        P  ++   ++ T   + +           L++ L LL+ D+ + ++ +   S G     + L+ ++ +    +  IVQ  +G    RIF  +L   ++E+    +  ++ A +   +L  MV++ F+T  E  K   Y       L+ V++ +  R   + CY+ +  L  R+  E  E KR     ++E +++I+ +    +    D          IT  + + +E + +  +K D  E  +   + L   F+Q
Sbjct:  244 RVNFERFHQHFHNQAIVSAVASKMDKTAGEIVRTMLRMSELTTPPNAAFTQLLASTEIFRSLPPEYG-MTKPVLDQYLSLLA-DDPMEFVTKSGESGGGTFIVNLLKSLKTMARCTLESIVQERFGSRAARIFRLLLQKRHLEQKQVEDFAMIPAKEAKEMLYTMVSQNFVTLQEIPKAADYAPARTFYLYTVNVEQTARFILQRCYKTVTNLIARRHHETKENKR-----LLEKAQRIEAILASVQASGADEAQLQEIEEMITATERQQLENLKRTVNKLDKSEMQVDETVFLLETFLQ 536
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Medaka
Match: polr3c (RNA polymerase III subunit C [Source:NCBI gene;Acc:101158381])

HSP 1 Score: 55.0694 bits (131), Expect = 1.221e-7
Identity = 74/344 (21.51%), Postives = 149/344 (43.31%), Query Frame = 2 
Query:  509 RNPNVTYTDEIKRILSTKENPTDSRKRVFEKID-ETESNIDKELRINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFIDSFDEEPVTSYEDPMSLTLFSKKVKKRL-ADFDDDK--LEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEV-EKKRVVLAHVIENSEKIKLV----KTDEEDPIK------DITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFI 1495
            R P VT     KR L+++E   D R     K+D ET  +     ++N ERF    R++  I      L + +  + +  +     E  TS   P +  L + ++ + L A ++  +  L++ L LL  D+ + ++ +   S G +   +  + +  L    +  IVQ  +G    RIF  +L   ++E+    +  ++ A +   +L  ++++  +   E  KT  +       L+ V+ L   R   + CY+ +  L  R+  E  E KR     ++E S++I+ +    +    +P +       IT  + + ++ +    +K DS E  +   + L  ++I
Sbjct:  201 RVPQVTLIGRSKRSLASEEG-EDQRNAKKAKMDPETHGDEGIYWQVNFERFHQHFRDQTIISAVANKLDQTSSEIVRTMLRM--SEVTTSSTAPCTKPLSANEIFRSLPATYNIPRHILDQYLTLLV-DDPMEFVGKAGESGGGMYIVNLNQALANLARATLESIVQERFGSRSARIFRLLLRKRHLEQKQVEDFAMIPAKEAKDMLYTLLSQNLVQLQEIPKTPDFAPSRTFYLYTVNQLPTARLLLQNCYKTVANLMERRLFEAKESKR-----LLEKSQRIEAILASLQASGAEPEQLTEVEEMITAPEKQQLDSLRLHVNKLDSAENQVDETIFLLESYI 535
[Close]
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Planmine SMEST
Match: SMESG000050553.1 (SMESG000050553.1)

HSP 1 Score: 1005.36 bits (2598), Expect = 0.000e+0
Identity = 501/504 (99.40%), Postives = 501/504 (99.40%), Query Frame = 2 
Query:   11 MSSSCVDFLLYEIQKNFNENSKNVCQILLSNNWSTLKEIHSELKHLKSKEILSCLENLIKYNIVIVNYDELHTDSYQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEEILENVPSNFQKRKTTKRNPNVTYTDEIKRILSTKENPTDSRKRVFEKIDETESNIDKELRINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFIDSFDEEPVTSYEDPMSLTLFSKKVKKRLADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEVEKKRVVLAHVIENSEKIKLVKTDEEDPIKDITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQQKVTYHNY 1522
            MSSSCVDFLLYEIQKNFNENSKNVCQILLSNNWSTLKEIHSELKHLKSKEILSCLENLIKYNIVIVNYDELHTDSYQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEEILENVPSNFQKRKTTKRNPNVTYTDEIKRILS KENPTDSRKRVFEKIDETESNIDKELRINNERFRVL RNEIFIQDAILTLGEDAGMVFKIFIDSFDEE VTSYEDPMSLTLFSKKVKKRLADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEVEKKRVVLAHVIENSEKIKLVKTDEEDPIKDITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQQKVTYHNY
Sbjct:    1 MSSSCVDFLLYEIQKNFNENSKNVCQILLSNNWSTLKEIHSELKHLKSKEILSCLENLIKYNIVIVNYDELHTDSYQFSIENFISYIISIRFLLIVNNKFDNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEEILENVPSNFQKRKTTKRNPNVTYTDEIKRILSNKENPTDSRKRVFEKIDETESNIDKELRINNERFRVLCRNEIFIQDAILTLGEDAGMVFKIFIDSFDEESVTSYEDPMSLTLFSKKVKKRLADFDDDKLEECLYLLSKDESVTYIDERLNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIEEMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHVDLLKIVRNERETCYQCLYRLGVRKEMEVEKKRVVLAHVIENSEKIKLVKTDEEDPIKDITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQQKVTYHNY 504
The following BLAST results are available for this feature:
BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
POLR3C3.480e-921.54RNA polymerase III subunit C [Source:HGNC Symbol;A... [more]
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
let-6119.142e-721.99pep chromosome:WBcel235:I:6255035:6257635:1 gene:W... [more]
let-6119.142e-721.99pep chromosome:WBcel235:I:6255035:6257791:1 gene:W... [more]
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
polr3c1.987e-1018.71polymerase (RNA) III (DNA directed) polypeptide C ... [more]
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q2TBL4|RPC3_BOVIN1.204e-920.52DNA-directed RNA polymerase III subunit RPC3 OS=Bo... [more]
sp|Q7ZUX1|RPC3_DANRE1.350e-918.71DNA-directed RNA polymerase III subunit RPC3 OS=Da... [more]
sp|Q9BUI4|RPC3_HUMAN1.671e-821.54DNA-directed RNA polymerase III subunit RPC3 OS=Ho... [more]
sp|Q55FJ5|RPC3_DICDI8.903e-625.85DNA-directed RNA polymerase III subunit rpc3 OS=Di... [more]
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1E1XC861.319e-1521.92RNA polymerase III subunit C3 (Fragment) OS=Amblyo... [more]
A0A5N4ARG81.241e-1423.14Uncharacterized protein OS=Photinus pyralis OX=705... [more]
A0A1Y1M8V11.260e-1423.14RNA polymerase III subunit C3 (Fragment) OS=Photin... [more]
G3ML893.217e-1421.54RNA polymerase III subunit C3 OS=Amblyomma maculat... [more]
A0A4D5RHN25.362e-1421.01RNA polymerase III subunit C3 (Fragment) OS=Ixodes... [more]
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
polr3c2.134e-619.37RNA polymerase III subunit C [Source:NCBI gene;Acc... [more]
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
polr3c8.951e-1019.67polymerase (RNA) III (DNA directed) polypeptide C ... [more]
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
polr3c1.221e-721.51RNA polymerase III subunit C [Source:NCBI gene;Acc... [more]
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BLAST of DNA-directed RNA polymerase III subunit RPC3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000050553.10.000e+099.40SMESG000050553.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035115 ID=SMED30035115|Name=DNA-directed RNA polymerase III subunit RPC3|organism=Schmidtea mediterranea sexual|type=transcript|length=1592bp
TTGAATACCAATGTCATCTTCATGTGTAGATTTTTTACTTTATGAAATTC
AGAAAAATTTTAATGAAAATAGTAAAAATGTATGTCAAATCTTGCTCTCA
AATAACTGGTCAACCTTAAAAGAAATACACAGTGAATTAAAACATCTTAA
ATCGAAAGAAATACTTAGTTGTCTGGAAAATCTAATAAAATATAATATTG
TTATCGTTAATTATGATGAGTTACATACTGATAGTTATCAATTTAGCATT
GAAAATTTCATTTCTTATATCATATCAATTCGATTTCTTCTAATCGTTAA
TAATAAATTTGATAATTTACACAAAATAATTTTTGAAATGTTTTACATGT
ATGGAAATTTATGCACATCAGAACTCCTTGATAAAATTAGAATATATATT
GAAGATGACGATTGGATTAATCTTGTTATTAATGCTACAGAAGTGTTAAT
AAATGAAGAAATATTAGAAAATGTACCATCAAACTTTCAAAAACGCAAAA
CAACAAAGAGAAATCCAAATGTCACATACACTGATGAAATAAAAAGAATT
TTATCAACTAAAGAAAATCCTACAGACAGTCGAAAAAGAGTTTTTGAAAA
GATTGATGAAACCGAAAGTAATATTGATAAGGAATTACGGATTAACAATG
AGCGATTCAGAGTTTTGAGTCGTAATGAAATTTTCATCCAAGATGCAATC
CTAACATTAGGAGAGGATGCGGGAATGGTTTTCAAAATCTTTATCGATTC
ATTCGACGAGGAGCCTGTAACCAGTTACGAAGACCCGATGTCATTGACTT
TGTTTTCGAAAAAAGTCAAAAAGCGACTCGCGGATTTCGATGATGACAAA
TTGGAAGAGTGCCTTTATTTATTGTCAAAAGACGAGTCAGTTACGTACAT
TGACGAGCGCCTAAACAGCAACGGGATTTTATTGTACTATGATCCTTTGA
GAATTGTCGAGCAGTTAGTGATGAAAATTATCACTGAGATTGTGCAAACG
ATTTACGGCGATAACGGACTGAGGATATTTCTTTTCGTTTTAAATAATCC
CTACATCGAGGAAATGAGTTTTCGGAATTTAGTAATCATGTCGGCCAATC
AAATAAATCCCCTATTAACAGCGATGGTTACTCGGGGATTCATAACGAGA
TGCGAATTTTCAAAGACAAAGCAGTATTTCGATGAGGCTTTGGTGTCGTT
ATTTCATGTGGATTTGTTGAAGATTGTTCGAAATGAAAGGGAGACTTGTT
ATCAGTGCCTATATCGATTGGGCGTCCGTAAAGAAATGGAAGTGGAGAAG
AAACGAGTTGTGTTGGCTCATGTGATTGAAAATAGTGAAAAAATTAAGCT
TGTGAAAACAGACGAGGAAGATCCCATTAAAGATATTACGAAAGATGATT
TAGAGGCTGTCGAATTCATTAACAAAGCTTCATCGAAATTTGATTCCTGT
GAATATGGCATTTATAGCAACCTGATTTTATTTAATGCTTTCATTCAGCA
GAAGGTCACGTACCACAATTACTAACATCTGTAATAAATTTGATATTGTT
CTTATTTTGAATGATTGGTTTGTTCTGTGTTCTTATGAGAAA
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protein sequence of SMED30035115-orf-1

>SMED30035115-orf-1 ID=SMED30035115-orf-1|Name=SMED30035115-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=505bp
MSSSCVDFLLYEIQKNFNENSKNVCQILLSNNWSTLKEIHSELKHLKSKE
ILSCLENLIKYNIVIVNYDELHTDSYQFSIENFISYIISIRFLLIVNNKF
DNLHKIIFEMFYMYGNLCTSELLDKIRIYIEDDDWINLVINATEVLINEE
ILENVPSNFQKRKTTKRNPNVTYTDEIKRILSTKENPTDSRKRVFEKIDE
TESNIDKELRINNERFRVLSRNEIFIQDAILTLGEDAGMVFKIFIDSFDE
EPVTSYEDPMSLTLFSKKVKKRLADFDDDKLEECLYLLSKDESVTYIDER
LNSNGILLYYDPLRIVEQLVMKIITEIVQTIYGDNGLRIFLFVLNNPYIE
EMSFRNLVIMSANQINPLLTAMVTRGFITRCEFSKTKQYFDEALVSLFHV
DLLKIVRNERETCYQCLYRLGVRKEMEVEKKRVVLAHVIENSEKIKLVKT
DEEDPIKDITKDDLEAVEFINKASSKFDSCEYGIYSNLILFNAFIQQKVT
YHNY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003697single-stranded DNA binding
GO:0003677DNA binding
GO:0003899DNA-directed 5'-3' RNA polymerase activity
Vocabulary: cellular component
TermDefinition
GO:0005666DNA-directed RNA polymerase III complex
Vocabulary: biological process
TermDefinition
GO:0032774RNA biosynthetic process
GO:0006351transcription, DNA-templated
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036388WH-like_DNA-bd_sf
IPR039748RPC3
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3DG3DSA:1.10.10.10coord: 1..78
e-value: 2.5E-5
score: 26.3
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3DG3DSA:1.10.10.10coord: 313..402
e-value: 1.3E-12
score: 49.4
IPR039748DNA-directed RNA polymerase III subunit RPC3PANTHERPTHR12949RNA POLYMERASE III DNA DIRECTED -RELATEDcoord: 1..492